Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008667

Physical Location & Seq

Genomic Coordinates Reverse (-)

35777461 .. 35780035

Gene Structure 2575 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1264000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGGCAGCTCCTCTGCCTCTCATTCTCAGAAACCCTCCTCTACGTCTCTGCACTCACCACCTACTCCCTCCTCTTTCCCACACCACCACCACCGCAACAACCACCGCCGAACCCTTCTCTCTGATTTCCTTCTCTTCCTACAACGCCAGGTCCTTCCCCATCCTGAAGTCCACCCAGATGGAAGGGGGTGAAATTACCGAGGAAGTTACAGAGACAACTGAAACCAATGTGATTGGGAAGAAGAAAGTATTTGTGGCTGGTGCCACTGGAAATACAGGTAAACGGATTGTTGAGCAGCTGCTGGCCAAGGGTTTCTCAGTTAAAGCCGGAGTTCGGAACTTGGAGAAAGCGAAAGCTAGCCTTCCCTCAGACAACGCAGCTCTTCAAATTGTGAAGGCAGATGTGACAGATGGTTCTGCTAAGTTAGCAGAAGCGATAAGCGATGGTTCAGATGCTGTGATTTGTGCCACTGGATTTAGCTATGGATGGGATGTGTTTGCTCCATGGAAGGTTGATAATTTTGGCACAGTGAATCTCGTTGAAGCATGCCGTAAACTTGGTGTGAACCGGTTTATTCTTGTGAGTTCCATCTTGGTCAATGGAGCAGCAATGGGGCAGATTCTCAACCCGGCTTACATATTTCTTAATGTTTTTGGACTCACCTTAATAGCAAAGCTTCAGGCAGAGAACTATATCAGGAGGTCTGGTATAAACTACACGATTATAAGGCCCGGTGGGTTGAAAAACGACCCTCCAACCGGAAACCTTGTAATGGAGCCAGAGGACACACTTTCGGAAGGCAGCATCTCTAGAGATCTGGTAGCAGAAGTAGCTGTAGAGGCATTAGCGAATCCTGAAGCGTCCTACAAGGTTGTGGAGATAGTTTCGCGGCCTGATGCACCCAAGCGTACGTGTGAGGATCTTTTCGGTTCCATCAAGCAACGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0010287 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAD_binding_10

Protein Analysis

316

Amino Acids

33.78

Weight (kDa)

6.85

Isoelectric Point (pI)

36.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	80 - 242	7.2e-18	NmrA-like family
Epimerase	PF01370	83 - 244	5.1e-13	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	84 - 197	7.8e-06	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	85 - 205	3.3e-09	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10	PF13460	87 - 285	5.3e-48	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008667)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34460
fragaria_vesca	FvH4_3g05850 FvH4_3g05850 FvH4_3g05850
malus_domestica	MD05G1286400.v1.1 MD05G1286500.v1.1 MD10G1264000.v1.1
prunus_persica	Prupe.4G078400_v2.0.a1 Prupe.4G078500_v2.0.a1
pyrus_communis	pycom05g26490 pycom10g22010
rosa_chinensis	RchiOBHm_Chr5g0013671 RchiOBHm_Chr6g0254821
rosa_laevigata	RLG00000009678 RLG00000032039
rosa_roxburghii	Rroxscaffold_1G00062930
rosa_rugosa	Rorug05G0011900 Rorug05G0012000
rosa_samantha	Rh5AG106400 Rh5BG102600 Rh5CG114500 Rh5DG101300
rosa_wichuraiana	Rw5G009280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	725
AccB1I	GGYRCC	1 cut(s)	260
AccII	CGCG	1 cut(s)	889
AciI	CCGC	3 cut(s)	93, 105, 889
AclWI	GGATC	1 cut(s)	927
AcoI	YGGCCR	1 cut(s)	303
AcuI	CTGAAG	3 cut(s)	185, 662, 876
AfaI	GTAC	1 cut(s)	910
AfiI	CCNNNNNNNGG	3 cut(s)	333, 758, 772
AflIII	ACRYGT	1 cut(s)	911
AgeI	ACCGGT	1 cut(s)	567
AgsI	TTSAA	3 cut(s)	386, 542, 742
AjnI	CCWGG	1 cut(s)	146
AluBI	AGCT	7 cut(s)	8, 298, 356, 380, 480, 676, 833
AluI	AGCT	7 cut(s)	8, 298, 356, 380, 480, 676, 833
Alw26I	GTCTC	2 cut(s)	50, 206
AlwI	GGATC	1 cut(s)	927
AlwNI	CAGNNNCTG	2 cut(s)	301, 455
AoxI	GGCC	3 cut(s)	303, 728, 890
ApeKI	GCWGC	6 cut(s)	5, 295, 298, 377, 605, 801
AsiGI	ACCGGT	1 cut(s)	567
AspS9I	GGNCC	2 cut(s)	150, 729
AsuC2I	CCSGG	2 cut(s)	629, 732
AsuHPI	GGTGA	3 cut(s)	47, 200, 652
AsuNHI	GCTAGC	1 cut(s)	356
AvaII	GGWCC	1 cut(s)	150
BalI	TGGCCA	1 cut(s)	305
BanI	GGYRCC	1 cut(s)	260
BbvI	GCAGC	6 cut(s)	17, 285, 307, 389, 617, 813
BccI	CCATC	7 cut(s)	167, 172, 404, 437, 480, 596, 941
BceAI	ACGGC	1 cut(s)	534
BciT130I	CCWGG	1 cut(s)	148
BcnI	CCSGG	2 cut(s)	629, 732
BcoDI	GTCTC	2 cut(s)	50, 206
BfaI	CTAG	2 cut(s)	357, 810
BfmI	CTRYAG	1 cut(s)	834
BglII	AGATCT	1 cut(s)	814
BisI	GCNGC	7 cut(s)	6, 296, 299, 378, 606, 802, 890
BlsI	GCNGC	7 cut(s)	7, 297, 300, 379, 607, 803, 891
Bme1390I	CCNGG	3 cut(s)	148, 629, 732
Bme18I	GGWCC	1 cut(s)	150
BmgT120I	GGNCC	2 cut(s)	150, 729
BmiI	GGNNCC	3 cut(s)	262, 777, 931
BmrFI	CCNGG	3 cut(s)	148, 629, 732
BmsI	GCATC	3 cut(s)	442, 813, 886
BmtI	GCTAGC	1 cut(s)	360
BpuMI	CCSGG	2 cut(s)	629, 732
BsaAI	YACGTR	1 cut(s)	912
BsaJI	CCNNGG	3 cut(s)	198, 306, 503
BsaWI	WCCGGW	2 cut(s)	567, 758
BsaXI	ACNNNNNCTCC	2 cut(s)	691, 721
Bsc4I	CCNNNNNNNGG	3 cut(s)	333, 758, 772
Bse118I	RCCGGY	1 cut(s)	567
Bse1I	ACTGG	2 cut(s)	271, 475
Bse3DI	GCAATG	1 cut(s)	615
BseBI	CCWGG	1 cut(s)	148
BseDI	CCNNGG	3 cut(s)	198, 306, 503
BseGI	GGATG	3 cut(s)	159, 491, 496
BseLI	CCNNNNNNNGG	3 cut(s)	333, 758, 772
BseMI	GCAATG	1 cut(s)	615
BseMII	CTCAG	3 cut(s)	40, 330, 381
BseNI	ACTGG	2 cut(s)	271, 475
BseRI	GAGGAG	2 cut(s)	27, 60
BseXI	GCAGC	6 cut(s)	17, 285, 307, 389, 617, 813
BsgI	GTGCAG	1 cut(s)	34
Bsh1236I	CGCG	1 cut(s)	889
BshFI	GGCC	3 cut(s)	305, 730, 892
BshNI	GGYRCC	1 cut(s)	260
BshTI	ACCGGT	1 cut(s)	567
BsiSI	CCGG	5 cut(s)	327, 568, 629, 732, 759
BsiWI	CGTACG	1 cut(s)	908
BslI	CCNNNNNNNGG	3 cut(s)	333, 758, 772
BsmAI	GTCTC	2 cut(s)	50, 206
BsmBI	CGTCTC	1 cut(s)	50
BsnI	GGCC	3 cut(s)	305, 730, 892
Bsp143I	GATC	2 cut(s)	814, 919
Bsp19I	CCATGG	1 cut(s)	503
BspACI	CCGC	3 cut(s)	93, 105, 889
BspANI	GGCC	3 cut(s)	305, 730, 892
BspCNI	CTCAG	3 cut(s)	39, 329, 380
BspFNI	CGCG	1 cut(s)	889
BspLI	GGNNCC	3 cut(s)	262, 777, 931
BspOI	GCTAGC	1 cut(s)	360
BspPI	GGATC	1 cut(s)	927
BspQI	GCTCTTC	1 cut(s)	387
BspT107I	GGYRCC	1 cut(s)	260
BsrDI	GCAATG	1 cut(s)	615
BsrFI	RCCGGY	1 cut(s)	567
BsrI	ACTGG	2 cut(s)	271, 475
BssAI	RCCGGY	1 cut(s)	567
BssECI	CCNNGG	3 cut(s)	198, 306, 503
BssMI	GATC	2 cut(s)	814, 919
BssT1I	CCWWGG	2 cut(s)	306, 503
Bst2UI	CCWGG	1 cut(s)	148
Bst4CI	ACNGT	2 cut(s)	529, 945
Bst6I	CTCTTC	2 cut(s)	139, 387
BstBAI	YACGTR	1 cut(s)	912
BstC8I	GCNNGC	3 cut(s)	303, 358, 547
BstDEI	CTNAG	4 cut(s)	26, 316, 367, 420
BstDSI	CCRYGG	1 cut(s)	503
BstF5I	GGATG	3 cut(s)	159, 491, 496
BstFNI	CGCG	1 cut(s)	889
BstKTI	GATC	2 cut(s)	817, 922
BstMAI	GTCTC	2 cut(s)	50, 206
BstMBI	GATC	2 cut(s)	814, 919
BstMWI	GCNNNNNNNGC	3 cut(s)	425, 830, 839
BstNI	CCWGG	1 cut(s)	148
BstNSI	RCATGY	1 cut(s)	549
BstSCI	CCNGG	3 cut(s)	146, 627, 730
BstSFI	CTRYAG	1 cut(s)	834
BstUI	CGCG	1 cut(s)	889
BstV1I	GCAGC	6 cut(s)	17, 285, 307, 389, 617, 813
BstX2I	RGATCY	2 cut(s)	814, 919
BstXI	CCANNNNNNTGG	1 cut(s)	178
BstYI	RGATCY	2 cut(s)	814, 919
BsuRI	GGCC	3 cut(s)	305, 730, 892
BtgI	CCRYGG	1 cut(s)	503
BtgZI	GCGATG	1 cut(s)	456
BtsCI	GGATG	3 cut(s)	159, 491, 496
BtsIMutI	CAGTG	3 cut(s)	264, 468, 534
Cac8I	GCNNGC	3 cut(s)	303, 358, 547
CaiI	CAGNNNCTG	2 cut(s)	301, 455
Cfr10I	RCCGGY	1 cut(s)	567
Cfr13I	GGNCC	2 cut(s)	150, 729
CseI	GACGC	1 cut(s)	849
Csp6I	GTAC	1 cut(s)	909
CspAI	ACCGGT	1 cut(s)	567
CviAII	CATG	2 cut(s)	504, 546
CviQI	GTAC	1 cut(s)	909
DdeI	CTNAG	4 cut(s)	26, 316, 367, 420
DpnI	GATC	2 cut(s)	816, 921
DpnII	GATC	2 cut(s)	814, 919
EaeI	YGGCCR	1 cut(s)	303
Eam1104I	CTCTTC	2 cut(s)	139, 387
EarI	CTCTTC	2 cut(s)	139, 387
Eco130I	CCWWGG	2 cut(s)	306, 503
Eco47I	GGWCC	1 cut(s)	150
Eco57I	CTGAAG	3 cut(s)	185, 662, 876
EcoO109I	RGGNCCY	1 cut(s)	150
EcoRII	CCWGG	1 cut(s)	146
EcoT14I	CCWWGG	2 cut(s)	306, 503
ErhI	CCWWGG	2 cut(s)	306, 503
Esp3I	CGTCTC	1 cut(s)	50
FaeI	CATG	2 cut(s)	507, 549
FaiI	YATR	7 cut(s)	483, 505, 547, 638, 693, 710, 725
FalI	AAGNNNNNCTT	2 cut(s)	345, 377
FatI	CATG	2 cut(s)	503, 545
Fnu4HI	GCNGC	7 cut(s)	6, 296, 299, 378, 606, 802, 890
FokI	GGATG	3 cut(s)	146, 498, 503
Fsp4HI	GCNGC	7 cut(s)	6, 296, 299, 378, 606, 802, 890
FspBI	CTAG	2 cut(s)	357, 810
GluI	GCNGC	7 cut(s)	6, 296, 299, 378, 606, 802, 890
HaeIII	GGCC	3 cut(s)	305, 730, 892
HapII	CCGG	5 cut(s)	327, 568, 629, 732, 759
HgaI	GACGC	1 cut(s)	849
Hin1II	CATG	2 cut(s)	507, 549
HindIII	AAGCTT	1 cut(s)	674
HinfI	GANTC	4 cut(s)	532, 619, 657, 850
HpaII	CCGG	5 cut(s)	327, 568, 629, 732, 759
HphI	GGTGA	3 cut(s)	47, 200, 652
Hpy166II	GTNNAC	4 cut(s)	171, 281, 554, 565
Hpy188I	TCNGA	6 cut(s)	29, 123, 336, 370, 451, 796
Hpy188III	TCNNGA	4 cut(s)	163, 697, 810, 854
Hpy8I	GTNNAC	4 cut(s)	171, 281, 554, 565
HpyAV	CCTTC	8 cut(s)	124, 139, 163, 176, 371, 388, 502, 791
HpyCH4III	ACNGT	2 cut(s)	529, 945
HpyCH4IV	ACGT	2 cut(s)	43, 911
HpyCH4V	TGCA	2 cut(s)	51, 899
HpyF10VI	GCNNNNNNNGC	3 cut(s)	425, 830, 839
HpyF3I	CTNAG	4 cut(s)	26, 316, 367, 420
HpySE526I	ACGT	2 cut(s)	43, 911
Hsp92II	CATG	2 cut(s)	507, 549
Kzo9I	GATC	2 cut(s)	814, 919
LguI	GCTCTTC	1 cut(s)	387
LmnI	GCTCC	4 cut(s)	13, 505, 602, 775
Lsp1109I	GCAGC	6 cut(s)	17, 285, 307, 389, 617, 813
LweI	GCATC	3 cut(s)	442, 813, 886
MaeI	CTAG	2 cut(s)	357, 810
MaeII	ACGT	2 cut(s)	43, 911
MaeIII	GTNAC	2 cut(s)	205, 403
MalI	GATC	2 cut(s)	816, 921
MboI	GATC	2 cut(s)	814, 919
MboII	GAAGA	4 cut(s)	126, 250, 253, 374
MflI	RGATCY	2 cut(s)	814, 919
MlsI	TGGCCA	1 cut(s)	305
MluCI	AATT	3 cut(s)	192, 387, 517
MluNI	TGGCCA	1 cut(s)	305
MlyI	GAGTC	1 cut(s)	651
MmeI	TCCRAC	1 cut(s)	779
Mox20I	TGGCCA	1 cut(s)	305
MscI	TGGCCA	1 cut(s)	305
MseI	TTAA	3 cut(s)	321, 645, 665
MslI	CAYNNNNRTG	1 cut(s)	176
Msp20I	TGGCCA	1 cut(s)	305
MspA1I	CMGCKG	1 cut(s)	298
MspI	CCGG	5 cut(s)	327, 568, 629, 732, 759
MspR9I	CCNGG	3 cut(s)	148, 629, 732
MvaI	CCWGG	1 cut(s)	148
MvnI	CGCG	1 cut(s)	889
MwoI	GCNNNNNNNGC	3 cut(s)	425, 830, 839
NciI	CCSGG	2 cut(s)	629, 732
NcoI	CCATGG	1 cut(s)	503
NdeII	GATC	2 cut(s)	814, 919
NheI	GCTAGC	1 cut(s)	356
NlaIII	CATG	2 cut(s)	507, 549
NlaIV	GGNNCC	3 cut(s)	262, 777, 931
NmuCI	GTSAC	1 cut(s)	403
NspI	RCATGY	1 cut(s)	549
PaeI	GCATGC	1 cut(s)	549
PciSI	GCTCTTC	1 cut(s)	387
PfeI	GAWTC	3 cut(s)	532, 619, 850
Pfl23II	CGTACG	1 cut(s)	908
PinAI	ACCGGT	1 cut(s)	567
PkrI	GCNGC	7 cut(s)	7, 297, 300, 379, 607, 803, 891
PleI	GAGTC	1 cut(s)	651
PpsI	GAGTC	1 cut(s)	651
Ppu21I	YACGTR	1 cut(s)	912
PpuMI	RGGWCCY	1 cut(s)	150
PsiI	TTATAA	1 cut(s)	725
Psp5II	RGGWCCY	1 cut(s)	150
Psp6I	CCWGG	1 cut(s)	146
PspGI	CCWGG	1 cut(s)	146
PspLI	CGTACG	1 cut(s)	908
PspN4I	GGNNCC	3 cut(s)	262, 777, 931
PspPI	GGNCC	2 cut(s)	150, 729
PspPPI	RGGWCCY	1 cut(s)	150
PstNI	CAGNNNCTG	2 cut(s)	301, 455
PsuI	RGATCY	2 cut(s)	814, 919
PvuII	CAGCTG	1 cut(s)	298
RsaI	GTAC	1 cut(s)	910
RsaNI	GTAC	1 cut(s)	909
RseI	CAYNNNNRTG	1 cut(s)	176
SapI	GCTCTTC	1 cut(s)	387
SaqAI	TTAA	3 cut(s)	321, 645, 665
SatI	GCNGC	7 cut(s)	6, 296, 299, 378, 606, 802, 890
Sau3AI	GATC	2 cut(s)	814, 919
Sau96I	GGNCC	2 cut(s)	150, 729
SchI	GAGTC	1 cut(s)	651
ScrFI	CCNGG	3 cut(s)	148, 629, 732
SfaNI	GCATC	3 cut(s)	442, 813, 886
SfcI	CTRYAG	1 cut(s)	834
SinI	GGWCC	1 cut(s)	150
SmiMI	CAYNNNNRTG	1 cut(s)	176
SphI	GCATGC	1 cut(s)	549
Sse9I	AATT	3 cut(s)	192, 387, 517
SsiI	CCGC	3 cut(s)	93, 105, 889
SspMI	CTAG	2 cut(s)	357, 810
StyD4I	CCNGG	3 cut(s)	146, 627, 730
StyI	CCWWGG	2 cut(s)	306, 503
TaaI	ACNGT	2 cut(s)	529, 945
TaiI	ACGT	2 cut(s)	46, 914
TasI	AATT	3 cut(s)	192, 387, 517
TauI	GCSGC	1 cut(s)	892
TfiI	GAWTC	3 cut(s)	532, 619, 850
Tru1I	TTAA	3 cut(s)	321, 645, 665
Tru9I	TTAA	3 cut(s)	321, 645, 665
TscAI	CASTG	3 cut(s)	271, 475, 534
TseFI	GTSAC	1 cut(s)	403
TseI	GCWGC	6 cut(s)	5, 295, 298, 377, 605, 801
Tsp45I	GTSAC	1 cut(s)	403
TspGWI	ACGGA	1 cut(s)	298
TspRI	CASTG	3 cut(s)	271, 475, 534
VpaK11BI	GGWCC	1 cut(s)	150
XbaI	TCTAGA	1 cut(s)	809
XceI	RCATGY	1 cut(s)	549
XspI	CTAG	2 cut(s)	357, 810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.