FvH4_3g05850

protein At2g34460, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008667
Physical Location & Seq
Reverse (-)
3402252 .. 3404450
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05850.t1

Sequence Viewer

Length: 993 bp
ATGGCAGCTCCGCTGCCTCTCATTCTCAGAAACCCTTCTCCTCTCTTTGCTCCGATCCCGACCAAACCTCCTCTCCGTCTCCCCAACACTCCCTCCCTCCGCCACCACTACACCGCTCACGCCACAACCAAGCCCTTTTCCGTCCTTTCACTCTTCTCCACCAGCACCACCAGATCATTGTCCCCCAGCCTCAACTCCTCCAAGATGGAAGGGAGTGTAACCACTGGGAATGAGATCACTGAGGAAGTTTCTGAGAGCACAACTGAAGCAAGTTTGAGGGGTTTGAAGAAGATATTTGTGGCTGGTGCTACTGGGAGCACTGGTAAAAGGATTGTTGAGCAGTTACTGGCAAGGGGTTATGCTGTGAAAGCCGGAGTTCGGGACTTGGAGAAAGCCAAAACTAGTCTTCCCAGCAGTAACCCTGCTCTCCAAATTGTTAAAGCAGATGTGACAGAGGGTTCTGCAAAGCTTGCAGAGGCAATTAGTAGTGATTCGGAAGCTGTGATTTGTGCGACGGGGTTTAGCTATGGATGGGATGTGTTTGCTCCATGGAAGGTTGATAATTTTGGGACAGTTAACCTTGTTGATGCATGCCGTAAAGTAGGTGTGATGAGGTTTATTCTGGTCAGTTCCATTTTGGTAAATGGAGCAGCAATGGGGCAGATTCTCAATCCAGCTTACATCTTTCTTAATATCTTTGGACTCACCTTAATTGCGAAGCTTCAGGCAGAGAATTATATCAGGAAGTCTGGTATAAATTACACAATAATAAGGCCTGGTGGGTTGAGAAATGAACCTCCATCAGGAAATCTTGTGATGGAGCCAGAGGACACTCTATCAGGAGGCACCATCTCCAGAGATCTGGTTGCTGAAGTAGCTATTGAGGCATTAGTCACTCCTGAAGCATCTTACAAGGTAGTCGAGATAGTTTCCAGTGCTGATGCCCCCAAGCGTACATACGAGGATCTATTTGGTTCGATTAAGCAACGGTGA

Protein Analysis

331

Amino Acids

35.28

Weight (kDa)

8.94

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 96 - 257 5.3e-17 NmrA-like family
Epimerase PF01370 98 - 259 1.7e-11 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 100 - 221 1.2e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 102 - 299 6.8e-44 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 116
AciI CCGC 3 cut(s) 11, 100, 114
AclWI GGATC 2 cut(s) 49, 972
AcuI CTGAAG 4 cut(s) 285, 707, 891, 921
AfaI GTAC 1 cut(s) 955
AfiI CCNNNNNNNGG 2 cut(s) 378, 803
AgsI TTSAA 1 cut(s) 286
AhlI ACTAGT 1 cut(s) 401
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 7 cut(s) 8, 469, 500, 525, 677, 721, 878
AluI AGCT 7 cut(s) 8, 469, 500, 525, 677, 721, 878
Alw21I GWGCWC 2 cut(s) 260, 320
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 2 cut(s) 49, 972
AlwNI CAGNNNCTG 1 cut(s) 346
AoxI GGCC 1 cut(s) 773
ApeKI GCWGC 3 cut(s) 5, 13, 650
Asp700I GAANNNNTTC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 697
BanI GGYRCC 1 cut(s) 845
BbsI GAAGAC 1 cut(s) 398
Bbv12I GWGCWC 2 cut(s) 260, 320
BbvI GCAGC 2 cut(s) 17, 662
BccI CCATC 5 cut(s) 199, 525, 808, 811, 857
BceAI ACGGC 1 cut(s) 579
BciT130I CCWGG 1 cut(s) 777
BcoDI GTCTC 1 cut(s) 83
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 1 cut(s) 402
BglII AGATCT 1 cut(s) 859
BisI GCNGC 3 cut(s) 6, 14, 651
BlsI GCNGC 3 cut(s) 7, 15, 652
Bme1390I CCNGG 1 cut(s) 777
BmiI GGNNCC 2 cut(s) 822, 847
BmrFI CCNGG 1 cut(s) 777
BmrI ACTGGG 2 cut(s) 234, 321
BmsI GCATC 3 cut(s) 577, 914, 931
BmuI ACTGGG 2 cut(s) 234, 321
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 838
BsaJI CCNNGG 1 cut(s) 548
BsaXI ACNNNNNCTCC 8 cut(s) 52, 57, 77, 82, 87, 107, 307, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 803
Bse1I ACTGG 5 cut(s) 229, 316, 325, 351, 933
Bse3DI GCAATG 1 cut(s) 660
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 2 cut(s) 536, 541
BseLI CCNNNNNNNGG 2 cut(s) 378, 803
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 3 cut(s) 40, 231, 243
BseNI ACTGG 5 cut(s) 229, 316, 325, 351, 933
BseRI GAGGAG 3 cut(s) 30, 60, 187
BseXI GCAGC 2 cut(s) 17, 662
BseYI CCCAGC 2 cut(s) 185, 410
BshFI GGCC 1 cut(s) 775
BshNI GGYRCC 1 cut(s) 845
BsiHKAI GWGCWC 2 cut(s) 260, 320
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 3 cut(s) 166, 395, 583
BslI CCNNNNNNNGG 2 cut(s) 378, 803
BsmAI GTCTC 1 cut(s) 83
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 3 cut(s) 166, 395, 583
BsnI GGCC 1 cut(s) 775
Bsp1286I GDGCHC 2 cut(s) 260, 320
Bsp143I GATC 5 cut(s) 54, 173, 234, 859, 964
Bsp19I CCATGG 1 cut(s) 548
BspACI CCGC 3 cut(s) 11, 100, 114
BspANI GGCC 1 cut(s) 775
BspCNI CTCAG 3 cut(s) 39, 232, 244
BspLI GGNNCC 2 cut(s) 822, 847
BspPI GGATC 2 cut(s) 49, 972
BspT107I GGYRCC 1 cut(s) 845
BsrBI CCGCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 660
BsrI ACTGG 5 cut(s) 229, 316, 325, 351, 933
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 5 cut(s) 54, 173, 234, 859, 964
BssT1I CCWWGG 1 cut(s) 548
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 574, 990
Bst6I CTCTTC 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 471, 592
BstDEI CTNAG 3 cut(s) 26, 240, 252
BstDSI CCRYGG 1 cut(s) 548
BstENI CCTNNNNNAGG 1 cut(s) 801
BstF5I GGATG 2 cut(s) 536, 541
BstKTI GATC 5 cut(s) 57, 176, 237, 862, 967
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 5 cut(s) 54, 173, 234, 859, 964
BstMWI GCNNNNNNNGC 4 cut(s) 368, 470, 875, 884
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 1 cut(s) 594
BstSCI CCNGG 1 cut(s) 775
BstV1I GCAGC 2 cut(s) 17, 662
BstV2I GAAGAC 1 cut(s) 398
BstX2I RGATCY 2 cut(s) 859, 964
BstXI CCANNNNNNTGG 1 cut(s) 862
BstYI RGATCY 2 cut(s) 859, 964
BsuRI GGCC 1 cut(s) 775
BtgI CCRYGG 1 cut(s) 548
BtsCI GGATG 2 cut(s) 536, 541
BtsIMutI CAGTG 4 cut(s) 222, 237, 318, 940
Cac8I GCNNGC 2 cut(s) 471, 592
CaiI CAGNNNCTG 1 cut(s) 346
Csp6I GTAC 1 cut(s) 954
CviAII CATG 2 cut(s) 549, 591
CviQI GTAC 1 cut(s) 954
DdeI CTNAG 3 cut(s) 26, 240, 252
DpnI GATC 5 cut(s) 56, 175, 236, 861, 966
DpnII GATC 5 cut(s) 54, 173, 234, 859, 964
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 548
Eco147I AGGCCT 1 cut(s) 775
Eco57I CTGAAG 4 cut(s) 285, 707, 891, 921
EcoNI CCTNNNNNAGG 1 cut(s) 801
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 548
EcoT22I ATGCAT 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 548
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 2 cut(s) 552, 594
FaiI YATR 7 cut(s) 360, 528, 550, 592, 738, 755, 958
FaqI GGGAC 3 cut(s) 166, 395, 583
FatI CATG 2 cut(s) 548, 590
Fnu4HI GCNGC 3 cut(s) 6, 14, 651
FokI GGATG 2 cut(s) 543, 548
Fsp4HI GCNGC 3 cut(s) 6, 14, 651
FspBI CTAG 1 cut(s) 402
GluI GCNGC 3 cut(s) 6, 14, 651
GsaI CCCAGC 2 cut(s) 189, 414
GsuI CTGGAG 1 cut(s) 838
HaeIII GGCC 1 cut(s) 775
HapII CCGG 1 cut(s) 372
Hin1II CATG 2 cut(s) 552, 594
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 2 cut(s) 467, 719
HinfI GANTC 3 cut(s) 491, 664, 702
HpaI GTTAAC 1 cut(s) 577
HpaII CCGG 1 cut(s) 372
HphI GGTGA 1 cut(s) 697
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 4 cut(s) 29, 54, 253, 496
Hpy188III TCNNGA 8 cut(s) 58, 380, 742, 804, 840, 855, 899, 922
Hpy8I GTNNAC 1 cut(s) 577
Hpy99I CGWCG 1 cut(s) 517
HpyAV CCTTC 3 cut(s) 45, 203, 547
HpyCH4III ACNGT 2 cut(s) 574, 990
HpyCH4V TGCA 3 cut(s) 464, 473, 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 368, 470, 875, 884
HpyF3I CTNAG 3 cut(s) 26, 240, 252
Hsp92II CATG 2 cut(s) 552, 594
KspAI GTTAAC 1 cut(s) 577
Kzo9I GATC 5 cut(s) 54, 173, 234, 859, 964
LmnI GCTCC 6 cut(s) 13, 55, 315, 550, 647, 820
Lsp1109I GCAGC 2 cut(s) 17, 662
LweI GCATC 3 cut(s) 577, 914, 931
MaeI CTAG 1 cut(s) 402
MaeIII GTNAC 5 cut(s) 217, 342, 416, 448, 892
MalI GATC 5 cut(s) 56, 175, 236, 861, 966
MbiI CCGCTC 1 cut(s) 116
MboI GATC 5 cut(s) 54, 173, 234, 859, 964
MboII GAAGA 4 cut(s) 145, 298, 301, 398
MflI RGATCY 2 cut(s) 859, 964
MhlI GDGCHC 2 cut(s) 260, 320
MluCI AATT 6 cut(s) 432, 480, 562, 711, 733, 757
MlyI GAGTC 1 cut(s) 696
Mph1103I ATGCAT 1 cut(s) 592
MroXI GAANNNNTTC 1 cut(s) 34
MseI TTAA 5 cut(s) 438, 576, 690, 710, 981
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 1 cut(s) 777
MvaI CCWGG 1 cut(s) 777
MwoI GCNNNNNNNGC 4 cut(s) 368, 470, 875, 884
NcoI CCATGG 1 cut(s) 548
NdeII GATC 5 cut(s) 54, 173, 234, 859, 964
NlaIII CATG 2 cut(s) 552, 594
NlaIV GGNNCC 2 cut(s) 822, 847
NmuCI GTSAC 2 cut(s) 448, 892
NsiI ATGCAT 1 cut(s) 592
NspI RCATGY 1 cut(s) 594
PaeI GCATGC 1 cut(s) 594
PceI AGGCCT 1 cut(s) 775
PdmI GAANNNNTTC 1 cut(s) 34
PfeI GAWTC 2 cut(s) 491, 664
PkrI GCNGC 3 cut(s) 7, 15, 652
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 775
PspFI CCCAGC 2 cut(s) 185, 410
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 2 cut(s) 822, 847
PstNI CAGNNNCTG 1 cut(s) 346
PsuI RGATCY 2 cut(s) 859, 964
RsaI GTAC 1 cut(s) 955
RsaNI GTAC 1 cut(s) 954
SaqAI TTAA 5 cut(s) 438, 576, 690, 710, 981
SatI GCNGC 3 cut(s) 6, 14, 651
Sau3AI GATC 5 cut(s) 54, 173, 234, 859, 964
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 1 cut(s) 777
SduI GDGCHC 2 cut(s) 260, 320
SfaNI GCATC 3 cut(s) 577, 914, 931
SpeI ACTAGT 1 cut(s) 401
SphI GCATGC 1 cut(s) 594
Sse9I AATT 6 cut(s) 432, 480, 562, 711, 733, 757
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 3 cut(s) 11, 100, 114
SspMI CTAG 1 cut(s) 402
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 1 cut(s) 775
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 2 cut(s) 574, 990
TaqI TCGA 2 cut(s) 921, 977
TasI AATT 6 cut(s) 432, 480, 562, 711, 733, 757
TfiI GAWTC 2 cut(s) 491, 664
Tru1I TTAA 5 cut(s) 438, 576, 690, 710, 981
Tru9I TTAA 5 cut(s) 438, 576, 690, 710, 981
TscAI CASTG 4 cut(s) 229, 244, 325, 940
TseFI GTSAC 2 cut(s) 448, 892
TseI GCWGC 3 cut(s) 5, 13, 650
Tsp45I GTSAC 2 cut(s) 448, 892
TspDTI ATGAA 1 cut(s) 807
TspGWI ACGGA 2 cut(s) 65, 130
TspRI CASTG 4 cut(s) 229, 244, 325, 940
XagI CCTNNNNNAGG 1 cut(s) 801
XceI RCATGY 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 1 cut(s) 402
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.