MD05G1286500.v1.1

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008667
Physical Location & Seq
Reverse (-)
41935141 .. 41936551
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1286500.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGCCACATTAGCGCAACGAGCATTGAATAACACTACAAATCAAACACCTTACATTAGCGCAACGAAATCAAGTGGAGTGCCATCGGGAAAAAGAAGATTTTTGTGGCAGGTGCTACTGGAAGTACCGGCAGAAGAATCGTTGAGCACCTACTGGCTAAAGGTGAAAGCAGACGTGACAGAGGGTTCTGCGAAGCTAGCAGATGCGATAAGCAATGATTCAGAAGCTGTGATATGTGCCACGGGATGTCGCCCTGGATGGAATTTGTATGCTCCTTGGAAGGTTGATAATTTGGGCACAATCAACCTTGTAGAGGCATGCCGGAAACTTGGTGTGACTAGGTTTATCCTTGTGAGTTCCATTTTAGTCAATGGAGCAGCAATGGGCCAGATTCTCAATCCGGCTTACATCTATCTTAATGTTTTTGGACTCGCCTTGATAGCAAAGCTTCGCGCAGAACAATATGTTAGGGAGTCGGGCATAAACTATACGATAATCAGGCCTGGCGGGTTGAGAGACGAGCCTCCAACTGGAAATCTTGTGATGGAGTCAGCGGACACTCTTTATCAAGGCACAATCTCAAGAGATCAGGTTGCAGAAGTAGCAGTTGCGGCACTAATCCGCCCTGAAGCATCTTACAAGGTTGTAGAAATAGTTTCGCCGCCTGACGCCCCCAGCGTTCATGTGGCGAACTTTTTAGTTCAATCGCTCGCCGGTGACCTCTGA

Protein Analysis

242

Amino Acids

25.98

Weight (kDa)

5.92

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 55 - 207 9.3e-31 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 100
AccII CGCG 1 cut(s) 453
AciI CCGC 5 cut(s) 507, 554, 611, 622, 662
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 669
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 313, 530
AgsI TTSAA 2 cut(s) 28, 704
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 2 cut(s) 253, 502
AluBI AGCT 3 cut(s) 196, 227, 448
AluI AGCT 3 cut(s) 196, 227, 448
Alw21I GWGCWC 1 cut(s) 149
Alw26I GTCTC 1 cut(s) 510
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 3 cut(s) 3, 385, 500
ApeKI GCWGC 1 cut(s) 377
ApoI RAATTY 1 cut(s) 262
AspLEI GCGC 3 cut(s) 16, 62, 455
AspS9I GGNCC 1 cut(s) 385
AsuHPI GGTGA 1 cut(s) 175
AsuNHI GCTAGC 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 299
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 389
BccI CCATC 3 cut(s) 91, 252, 538
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciT130I CCWGG 2 cut(s) 255, 504
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 2 cut(s) 197, 339
BfuAI ACCTGC 1 cut(s) 100
BisI GCNGC 3 cut(s) 378, 612, 662
BlsI GCNGC 3 cut(s) 379, 613, 663
Bme1390I CCNGG 2 cut(s) 255, 504
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 385
BmrFI CCNGG 2 cut(s) 255, 504
BmsI GCATC 2 cut(s) 193, 641
BmtI GCTAGC 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 669
BsaJI CCNNGG 3 cut(s) 240, 253, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 530
Bse118I RCCGGY 2 cut(s) 127, 713
Bse1I ACTGG 3 cut(s) 123, 158, 535
Bse3DI GCAATG 2 cut(s) 220, 387
BseBI CCWGG 2 cut(s) 255, 504
BseDI CCNNGG 3 cut(s) 240, 253, 275
BseGI GGATG 2 cut(s) 251, 263
BseLI CCNNNNNNNGG 2 cut(s) 313, 530
BseMI GCAATG 2 cut(s) 220, 387
BseNI ACTGG 3 cut(s) 123, 158, 535
BseSI GKGCMC 1 cut(s) 299
BseXI GCAGC 1 cut(s) 389
BseYI CCCAGC 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 453
BshFI GGCC 3 cut(s) 5, 387, 502
BsiHKAI GWGCWC 1 cut(s) 149
BsiSI CCGG 4 cut(s) 128, 322, 401, 714
BslI CCNNNNNNNGG 2 cut(s) 313, 530
BsmAI GTCTC 1 cut(s) 510
BsmBI CGTCTC 1 cut(s) 510
BsnI GGCC 3 cut(s) 5, 387, 502
Bsp1286I GDGCHC 2 cut(s) 149, 299
Bsp143I GATC 1 cut(s) 586
BspACI CCGC 5 cut(s) 507, 554, 611, 622, 662
BspANI GGCC 3 cut(s) 5, 387, 502
BspFNI CGCG 1 cut(s) 453
BspMI ACCTGC 1 cut(s) 100
BspOI GCTAGC 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 220, 387
BsrFI RCCGGY 2 cut(s) 127, 713
BsrI ACTGG 3 cut(s) 123, 158, 535
BssAI RCCGGY 2 cut(s) 127, 713
BssECI CCNNGG 3 cut(s) 240, 253, 275
BssMI GATC 1 cut(s) 586
BssNI GRCGYC 1 cut(s) 669
BssT1I CCWWGG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 255, 504
BstACI GRCGYC 1 cut(s) 669
BstC8I GCNNGC 3 cut(s) 198, 319, 711
BstDSI CCRYGG 1 cut(s) 240
BstEII GGTNACC 1 cut(s) 716
BstENI CCTNNNNNAGG 1 cut(s) 311
BstF5I GGATG 2 cut(s) 251, 263
BstFNI CGCG 1 cut(s) 453
BstHHI GCGC 3 cut(s) 16, 62, 455
BstKTI GATC 1 cut(s) 589
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 1 cut(s) 586
BstMWI GCNNNNNNNGC 6 cut(s) 11, 20, 197, 440, 602, 611
BstNI CCWGG 2 cut(s) 255, 504
BstNSI RCATGY 1 cut(s) 321
BstPI GGTNACC 1 cut(s) 716
BstSCI CCNGG 2 cut(s) 253, 502
BstSLI GKGCMC 1 cut(s) 299
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 389
BsuRI GGCC 3 cut(s) 5, 387, 502
BtgI CCRYGG 1 cut(s) 240
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 2 cut(s) 251, 263
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 3 cut(s) 198, 319, 711
CaiI CAGNNNCTG 1 cut(s) 227
CfoI GCGC 3 cut(s) 16, 62, 455
Cfr10I RCCGGY 2 cut(s) 127, 713
Cfr13I GGNCC 1 cut(s) 385
CseI GACGC 1 cut(s) 677
Csp6I GTAC 1 cut(s) 125
CviAII CATG 2 cut(s) 318, 683
CviJI RGCY 9 cut(s) 5, 157, 196, 227, 387, 404, 448, 502, 523
CviKI_1 RGCY 9 cut(s) 5, 157, 196, 227, 387, 404, 448, 502, 523
CviQI GTAC 1 cut(s) 125
DpnI GATC 1 cut(s) 588
DpnII GATC 1 cut(s) 586
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 611
Eco130I CCWWGG 1 cut(s) 275
Eco147I AGGCCT 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 648
Eco91I GGTNACC 1 cut(s) 716
EcoNI CCTNNNNNAGG 1 cut(s) 311
EcoO65I GGTNACC 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 253, 502
EcoT14I CCWWGG 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 275
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 2 cut(s) 321, 686
FaiI YATR 7 cut(s) 235, 270, 319, 465, 482, 489, 684
FatI CATG 2 cut(s) 317, 682
FauI CCCGC 1 cut(s) 500
Fnu4HI GCNGC 3 cut(s) 378, 612, 662
FokI GGATG 2 cut(s) 258, 270
Fsp4HI GCNGC 3 cut(s) 378, 612, 662
FspBI CTAG 2 cut(s) 197, 339
GlaI GCGC 3 cut(s) 15, 61, 454
GluI GCNGC 3 cut(s) 378, 612, 662
GsaI CCCAGC 1 cut(s) 678
HaeIII GGCC 3 cut(s) 5, 387, 502
HapII CCGG 4 cut(s) 128, 322, 401, 714
HgaI GACGC 1 cut(s) 677
HhaI GCGC 3 cut(s) 16, 62, 455
Hin1I GRCGYC 1 cut(s) 669
Hin1II CATG 2 cut(s) 321, 686
Hin6I GCGC 3 cut(s) 14, 60, 453
HinP1I GCGC 3 cut(s) 14, 60, 453
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 6 cut(s) 137, 218, 391, 429, 473, 548
HpaII CCGG 4 cut(s) 128, 322, 401, 714
HphI GGTGA 1 cut(s) 175
Hpy188I TCNGA 2 cut(s) 223, 725
Hpy188III TCNNGA 2 cut(s) 87, 582
HpyAV CCTTC 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 1 cut(s) 596
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 20, 197, 440, 602, 611
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 669
Hsp92II CATG 2 cut(s) 321, 686
HspAI GCGC 3 cut(s) 14, 60, 453
Kzo9I GATC 1 cut(s) 586
LmnI GCTCC 2 cut(s) 277, 374
Lsp1109I GCAGC 1 cut(s) 389
LweI GCATC 2 cut(s) 193, 641
MaeI CTAG 2 cut(s) 197, 339
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 175, 334, 716
MalI GATC 1 cut(s) 588
MboI GATC 1 cut(s) 586
MboII GAAGA 2 cut(s) 108, 146
MhlI GDGCHC 2 cut(s) 149, 299
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 262, 289
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 423, 482, 557
MmeI TCCRAC 1 cut(s) 551
MnlI CCTC 3 cut(s) 175, 307, 534
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 4 cut(s) 128, 322, 401, 714
MspR9I CCNGG 2 cut(s) 255, 504
MvaI CCWGG 2 cut(s) 255, 504
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 6 cut(s) 11, 20, 197, 440, 602, 611
NdeII GATC 1 cut(s) 586
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 2 cut(s) 321, 686
NmuCI GTSAC 3 cut(s) 175, 334, 716
NspI RCATGY 1 cut(s) 321
PaeI GCATGC 1 cut(s) 321
PaqCI CACCTGC 1 cut(s) 100
PceI AGGCCT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PfeI GAWTC 3 cut(s) 137, 218, 391
PkrI GCNGC 3 cut(s) 379, 613, 663
PleI GAGTC 3 cut(s) 423, 481, 556
PpsI GAGTC 3 cut(s) 423, 481, 556
Psp6I CCWGG 2 cut(s) 253, 502
PspEI GGTNACC 1 cut(s) 716
PspFI CCCAGC 1 cut(s) 674
PspGI CCWGG 2 cut(s) 253, 502
PspPI GGNCC 1 cut(s) 385
PstNI CAGNNNCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 3 cut(s) 378, 612, 662
Sau3AI GATC 1 cut(s) 586
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 3 cut(s) 423, 482, 557
ScrFI CCNGG 2 cut(s) 255, 504
SduI GDGCHC 2 cut(s) 149, 299
SfaNI GCATC 2 cut(s) 193, 641
SgrAI CRCCGGYG 1 cut(s) 713
SmlI CTYRAG 1 cut(s) 580
SmoI CTYRAG 1 cut(s) 580
SphI GCATGC 1 cut(s) 321
Sse9I AATT 2 cut(s) 262, 289
SseBI AGGCCT 1 cut(s) 502
SsiI CCGC 5 cut(s) 507, 554, 611, 622, 662
SspMI CTAG 2 cut(s) 197, 339
StuI AGGCCT 1 cut(s) 502
StyD4I CCNGG 2 cut(s) 253, 502
StyI CCWWGG 1 cut(s) 275
TaiI ACGT 1 cut(s) 177
TasI AATT 2 cut(s) 262, 289
TauI GCSGC 2 cut(s) 614, 664
TfiI GAWTC 3 cut(s) 137, 218, 391
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseFI GTSAC 3 cut(s) 175, 334, 716
TseI GCWGC 1 cut(s) 377
Tsp45I GTSAC 3 cut(s) 175, 334, 716
TspDTI ATGAA 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 311
XapI RAATTY 1 cut(s) 262
XceI RCATGY 1 cut(s) 321
XcmI CCANNNNNNNNNTGG 1 cut(s) 682
XspI CTAG 2 cut(s) 197, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.