Rh5AG106400

protein At2g34460, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008667
Physical Location & Seq
Forward (+)
9747986 .. 9750422
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG106400.1

Sequence Viewer

Length: 1002 bp
ATGGCAGCTCCGCTGCCTCTCATTCTCAGAAACCCTTGTTCTCTCTTAGCTCCGATCCCGACGAAGCCTCCTCTCCGTCTCCCCAACACTCACTCCCACACCCGCCACTACTCTCCTCTCTCCACAACCAAGCCCTTTTCCGTCATTTCACTCTCCTCCTCCTCCACCACCACCACCAGACCCTTATTCCCCATCCTCAACTCCTCAAAGATGGAAGGGGGTGAAACCACTGGGAATGAAATCACAGAGGAAGTTACTGAGACCACAACTGAAGCAACAAGTGTGAATGGTTTGAAGAAGATTTTTGTGGCTGGTGCTACTGGGAGCACTGGTAAAAGAATTGTTGAGCAGTTACTGGCTAAGGGTTATGGGGTAAAAGCCGGAGTTCGGGACTTGGAAAAAGCCAAAACTAGTCTTCCAAGCAACAACCCAGCTCTTGAAATTGTGAAAGCAGATGTGACGGAGGGTTCTGCGAAGTTGGCAGAAGCAATTAGTAGTGATTCGGAAGCTGTGATTTGCGCTACGGGGTTTAGCTATGGATGGGATGTGTTTGCTCCATGGAAGGTTGATAATTTTGGGACAGTTAACCTTGTCGATGCATGCCGTAAAGTTGGTGTGAAGAGGTTTATTCTGGTCAGTTCCATTTTAGTCAATGGAGCATCAATGGGGCAGATTCTCAATCCGGCTTACATTTTTCTTAATATATTTGGACTCACTTTAATCGCAAAGCTTCAGGCAGAGAATTATATCAGGAAGTCTGGTATAAACTACACAATAATAAGGCCTGGTGGGTTGAGAAATGAACCTCCAACAGGGAATCTTGTGATGGAGCCAGAGGACACTCTATCTGGAGGCACCATCTCCAGAGATCTGGTTGCGGAAGTAGCTGTTGAGGCATTGGTCACTCCTGAAGCATCTTACAAGGTAGTTGAGATAGTTTCCAGTGCTGATGCCCCCAAGCGTACATACAAGGAACTATTTGGTTCGGTTAAGCAACGGTGA

Protein Analysis

333

Amino Acids

35.58

Weight (kDa)

8.82

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 99 - 260 2.6e-18 NmrA-like family
Epimerase PF01370 101 - 262 1.8e-12 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 103 - 225 1.6e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 105 - 302 3.6e-45 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 854
AciI CCGC 3 cut(s) 11, 103, 878
AclWI GGATC 1 cut(s) 49
AcuI CTGAAG 3 cut(s) 291, 716, 930
AfaI GTAC 1 cut(s) 964
AfiI CCNNNNNNNGG 2 cut(s) 387, 812
AgsI TTSAA 2 cut(s) 295, 440
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 1 cut(s) 784
AluBI AGCT 7 cut(s) 8, 50, 434, 509, 534, 730, 887
AluI AGCT 7 cut(s) 8, 50, 434, 509, 534, 730, 887
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 2 cut(s) 83, 254
AlwI GGATC 1 cut(s) 49
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 1 cut(s) 782
ApeKI GCWGC 2 cut(s) 5, 13
AspLEI GCGC 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 233
BanI GGYRCC 1 cut(s) 854
BbsI GAAGAC 1 cut(s) 407
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 1 cut(s) 17
BccI CCATC 5 cut(s) 200, 205, 534, 820, 866
BceAI ACGGC 1 cut(s) 588
BciT130I CCWGG 1 cut(s) 786
BcoDI GTCTC 2 cut(s) 83, 254
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 1 cut(s) 411
BglII AGATCT 1 cut(s) 868
BisI GCNGC 2 cut(s) 6, 14
BlsI GCNGC 2 cut(s) 7, 15
Bme1390I CCNGG 1 cut(s) 786
BmiI GGNNCC 2 cut(s) 831, 856
BmrFI CCNGG 1 cut(s) 786
BmrI ACTGGG 2 cut(s) 240, 330
BmsI GCATC 4 cut(s) 586, 668, 923, 940
BmuI ACTGGG 2 cut(s) 240, 330
BpiI GAAGAC 1 cut(s) 407
BpmI CTGGAG 2 cut(s) 847, 870
Bpu10I CCTNAGC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 557
BsaXI ACNNNNNCTCC 6 cut(s) 52, 77, 82, 107, 316, 346
Bsc4I CCNNNNNNNGG 2 cut(s) 387, 812
Bse1I ACTGG 5 cut(s) 235, 325, 334, 360, 942
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 3 cut(s) 192, 545, 550
BseLI CCNNNNNNNGG 2 cut(s) 387, 812
BseMII CTCAG 2 cut(s) 40, 249
BseNI ACTGG 5 cut(s) 235, 325, 334, 360, 942
BseRI GAGGAG 6 cut(s) 60, 105, 145, 148, 151, 193
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 430
BshFI GGCC 1 cut(s) 784
BshNI GGYRCC 1 cut(s) 854
BsiHKAI GWGCWC 1 cut(s) 329
BsiSI CCGG 2 cut(s) 381, 683
BslFI GGGAC 2 cut(s) 404, 592
BslI CCNNNNNNNGG 2 cut(s) 387, 812
BsmAI GTCTC 2 cut(s) 83, 254
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 404, 592
BsnI GGCC 1 cut(s) 784
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 2 cut(s) 54, 868
Bsp19I CCATGG 1 cut(s) 557
BspACI CCGC 3 cut(s) 11, 103, 878
BspANI GGCC 1 cut(s) 784
BspCNI CTCAG 2 cut(s) 39, 250
BspLI GGNNCC 2 cut(s) 831, 856
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 854
BspTNI GGTCTC 1 cut(s) 254
BsrI ACTGG 5 cut(s) 235, 325, 334, 360, 942
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 2 cut(s) 54, 868
BssT1I CCWWGG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 2 cut(s) 583, 999
Bst6I CTCTTC 1 cut(s) 614
BstC8I GCNNGC 1 cut(s) 601
BstDEI CTNAG 4 cut(s) 26, 46, 258, 360
BstDSI CCRYGG 1 cut(s) 557
BstENI CCTNNNNNAGG 1 cut(s) 810
BstF5I GGATG 3 cut(s) 192, 545, 550
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 2 cut(s) 57, 871
BstMAI GTCTC 2 cut(s) 83, 254
BstMBI GATC 2 cut(s) 54, 868
BstMWI GCNNNNNNNGC 3 cut(s) 479, 884, 893
BstNI CCWGG 1 cut(s) 786
BstNSI RCATGY 1 cut(s) 603
BstSCI CCNGG 1 cut(s) 784
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 407
BstX2I RGATCY 1 cut(s) 868
BstXI CCANNNNNNTGG 1 cut(s) 871
BstYI RGATCY 1 cut(s) 868
BsuRI GGCC 1 cut(s) 784
BtgI CCRYGG 1 cut(s) 557
BtsCI GGATG 3 cut(s) 192, 545, 550
BtsIMutI CAGTG 3 cut(s) 228, 327, 949
Cac8I GCNNGC 1 cut(s) 601
CaiI CAGNNNCTG 1 cut(s) 355
CfoI GCGC 1 cut(s) 521
Csp6I GTAC 1 cut(s) 963
CviAII CATG 2 cut(s) 558, 600
CviQI GTAC 1 cut(s) 963
DdeI CTNAG 4 cut(s) 26, 46, 258, 360
DpnI GATC 2 cut(s) 56, 870
DpnII GATC 2 cut(s) 54, 868
Eam1104I CTCTTC 1 cut(s) 614
EarI CTCTTC 1 cut(s) 614
Eco130I CCWWGG 1 cut(s) 557
Eco147I AGGCCT 1 cut(s) 784
Eco31I GGTCTC 1 cut(s) 254
Eco57I CTGAAG 3 cut(s) 291, 716, 930
EcoNI CCTNNNNNAGG 1 cut(s) 810
EcoRII CCWGG 1 cut(s) 784
EcoT14I CCWWGG 1 cut(s) 557
EcoT22I ATGCAT 1 cut(s) 601
ErhI CCWWGG 1 cut(s) 557
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 2 cut(s) 561, 603
FaiI YATR 8 cut(s) 369, 537, 559, 601, 704, 747, 764, 967
FaqI GGGAC 2 cut(s) 404, 592
FatI CATG 2 cut(s) 557, 599
FauI CCCGC 1 cut(s) 110
Fnu4HI GCNGC 2 cut(s) 6, 14
FokI GGATG 3 cut(s) 179, 552, 557
Fsp4HI GCNGC 2 cut(s) 6, 14
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 520
GluI GCNGC 2 cut(s) 6, 14
GsaI CCCAGC 1 cut(s) 434
GsuI CTGGAG 2 cut(s) 847, 870
HaeIII GGCC 1 cut(s) 784
HapII CCGG 2 cut(s) 381, 683
HhaI GCGC 1 cut(s) 521
Hin1II CATG 2 cut(s) 561, 603
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 4 cut(s) 500, 673, 711, 817
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 2 cut(s) 381, 683
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 3 cut(s) 29, 54, 505
Hpy188III TCNNGA 7 cut(s) 58, 389, 437, 751, 849, 864, 908
Hpy8I GTNNAC 1 cut(s) 586
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 209, 556
HpyCH4III ACNGT 2 cut(s) 583, 999
HpyCH4V TGCA 1 cut(s) 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 479, 884, 893
HpyF3I CTNAG 4 cut(s) 26, 46, 258, 360
Hsp92II CATG 2 cut(s) 561, 603
HspAI GCGC 1 cut(s) 519
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 2 cut(s) 54, 868
LmnI GCTCC 6 cut(s) 13, 55, 324, 559, 656, 829
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 4 cut(s) 586, 668, 923, 940
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 4 cut(s) 253, 351, 457, 901
MalI GATC 2 cut(s) 56, 870
MboI GATC 2 cut(s) 54, 868
MboII GAAGA 4 cut(s) 307, 310, 407, 631
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 1 cut(s) 329
MluCI AATT 5 cut(s) 339, 441, 489, 571, 742
MlyI GAGTC 1 cut(s) 705
MmeI TCCRAC 1 cut(s) 833
Mph1103I ATGCAT 1 cut(s) 601
MseI TTAA 4 cut(s) 585, 699, 719, 990
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 2 cut(s) 381, 683
MspR9I CCNGG 1 cut(s) 786
MvaI CCWGG 1 cut(s) 786
MwoI GCNNNNNNNGC 3 cut(s) 479, 884, 893
NcoI CCATGG 1 cut(s) 557
NdeII GATC 2 cut(s) 54, 868
NlaIII CATG 2 cut(s) 561, 603
NlaIV GGNNCC 2 cut(s) 831, 856
NmuCI GTSAC 2 cut(s) 457, 901
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 1 cut(s) 603
PaeI GCATGC 1 cut(s) 603
PceI AGGCCT 1 cut(s) 784
PfeI GAWTC 3 cut(s) 500, 673, 817
PkrI GCNGC 2 cut(s) 7, 15
PleI GAGTC 1 cut(s) 705
PpsI GAGTC 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 784
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 2 cut(s) 831, 856
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 868
RsaI GTAC 1 cut(s) 964
RsaNI GTAC 1 cut(s) 963
SaqAI TTAA 4 cut(s) 585, 699, 719, 990
SatI GCNGC 2 cut(s) 6, 14
Sau3AI GATC 2 cut(s) 54, 868
SchI GAGTC 1 cut(s) 705
ScrFI CCNGG 1 cut(s) 786
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 4 cut(s) 586, 668, 923, 940
SpeI ACTAGT 1 cut(s) 410
SphI GCATGC 1 cut(s) 603
Sse9I AATT 5 cut(s) 339, 441, 489, 571, 742
SseBI AGGCCT 1 cut(s) 784
SsiI CCGC 3 cut(s) 11, 103, 878
SspMI CTAG 1 cut(s) 411
StuI AGGCCT 1 cut(s) 784
StyD4I CCNGG 1 cut(s) 784
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 2 cut(s) 583, 999
TaqI TCGA 1 cut(s) 594
TasI AATT 5 cut(s) 339, 441, 489, 571, 742
TfiI GAWTC 3 cut(s) 500, 673, 817
Tru1I TTAA 4 cut(s) 585, 699, 719, 990
Tru9I TTAA 4 cut(s) 585, 699, 719, 990
TscAI CASTG 3 cut(s) 235, 334, 949
TseFI GTSAC 2 cut(s) 457, 901
TseI GCWGC 2 cut(s) 5, 13
Tsp45I GTSAC 2 cut(s) 457, 901
TspDTI ATGAA 2 cut(s) 252, 816
TspGWI ACGGA 3 cut(s) 65, 130, 476
TspRI CASTG 3 cut(s) 235, 334, 949
XagI CCTNNNNNAGG 1 cut(s) 810
XceI RCATGY 1 cut(s) 603
XspI CTAG 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.