RchiOBHm_Chr5g0013671

protein At2g34460, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008667
Physical Location & Seq
Forward (+)
9207076 .. 9210545
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29418

Sequence Viewer

Length: 996 bp
ATGGCAGCTCCGCTGCCTCTCATTCTCAGAAACCCTTCTTCTCTCTTAGCTCCGATCCCGACGAACCCTCCTCTCCGTCTCCCCAACACTCACTCCCAGAGCCACCACTACTCCCCTCTCTCCACAACCAAACCCTTTTCCGTCATTTCACTCTCCTCCTCCTCCACTACCAGGCCCTTATTCCCCATCCTTAACTCCTCTAAGATGGAAGGGAGTGAAACCACTGGGAATGAAATCACAGAGGAAGTTACTGAGACCACAACTGAAGCAACAAGTGTGAATGGTTTGAAGAAGATTTTTGTGGCTGGTGCTACTGGGAGCACTGGTAAAAGAATTGTTGAGCAGTTACTGGCTAGGGGTTATGGAGTAAAAGCTGGAGTTCGGGACTTGGAAAAAGCCAAAACTAGTCTTCCAAGCAACAACCCAGCTCTTGAAATTGTGAAAGCAGATGTGACAGAGGGTTCTGCGAAGTTGGCAGAAGCAATTAGTAATGATTCGGAAGCTGTGATTTGCGCCACGGGGTTTAGCTATGGATGGGATGTGTTTGCTCCATGGAAGGTTGATAATTTTGGGACAGTTAACCTTGTCGATGCATGCCGTAAAGTTGGTGTGAAGAGGTTTATTCTGGTCAGTTCCATTTTAGTCAATGGAGCATCAATGGGGCAGATTCTCAATCCGGCTTACATTTTTCTTAATATATTTGGACTCACTTTAATCGCAAAGCTTCAGGCAGAGAATTATATCAGGAAGTCTGGTATAAACTACACAATAATAAGGCCTGGCGGGTTGAGAAATGAACCTCCAACAGGGAATCTTGTGATGGAGCCAGAGGACACTCTATCTGGAGGCACCATCTCCAGGGATCTGGTTGCGGAAGTAGCTGTTGAGGCATTGGTCACTCCTGAAGCATCTTACAAGGTAGTTGAGATAGTTTCCAGTGCTGATGCCCCCAAGCGTACATACAAGGAACTATTTGGTTCGGTTAAGCAACGGTGA

Protein Analysis

331

Amino Acids

35.39

Weight (kDa)

8.39

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 97 - 258 2.6e-18 NmrA-like family
Epimerase PF01370 99 - 260 2.1e-12 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 101 - 223 8e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 103 - 300 1.5e-45 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 848
AciI CCGC 3 cut(s) 11, 783, 872
AclWI GGATC 2 cut(s) 49, 870
AcuI CTGAAG 3 cut(s) 285, 710, 924
AfaI GTAC 1 cut(s) 958
AfiI CCNNNNNNNGG 3 cut(s) 171, 806, 858
AgsI TTSAA 2 cut(s) 289, 434
AhlI ACTAGT 1 cut(s) 404
AjnI CCWGG 3 cut(s) 170, 778, 857
AluBI AGCT 8 cut(s) 8, 50, 374, 428, 503, 528, 724, 881
AluI AGCT 8 cut(s) 8, 50, 374, 428, 503, 528, 724, 881
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 2 cut(s) 83, 248
AlwI GGATC 2 cut(s) 49, 870
AlwNI CAGNNNCTG 1 cut(s) 349
AoxI GGCC 2 cut(s) 173, 776
ApeKI GCWGC 2 cut(s) 5, 13
Asp700I GAANNNNTTC 1 cut(s) 34
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 848
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 17
BccI CCATC 5 cut(s) 194, 199, 528, 814, 860
BceAI ACGGC 1 cut(s) 582
BciT130I CCWGG 3 cut(s) 172, 780, 859
BcoDI GTCTC 2 cut(s) 83, 248
BcuI ACTAGT 1 cut(s) 404
BfaI CTAG 2 cut(s) 354, 405
BisI GCNGC 2 cut(s) 6, 14
BlsI GCNGC 2 cut(s) 7, 15
Bme1390I CCNGG 3 cut(s) 172, 780, 859
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 825, 850
BmrFI CCNGG 3 cut(s) 172, 780, 859
BmrI ACTGGG 2 cut(s) 234, 324
BmsI GCATC 4 cut(s) 580, 662, 917, 934
BmuI ACTGGG 2 cut(s) 234, 324
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 3 cut(s) 396, 841, 864
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 3 cut(s) 516, 551, 858
BsaXI ACNNNNNCTCC 8 cut(s) 52, 77, 82, 95, 107, 125, 310, 340
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 806, 858
Bse1I ACTGG 5 cut(s) 229, 319, 328, 354, 936
BseBI CCWGG 3 cut(s) 172, 780, 859
BseDI CCNNGG 3 cut(s) 516, 551, 858
BseGI GGATG 3 cut(s) 186, 539, 544
BseLI CCNNNNNNNGG 3 cut(s) 171, 806, 858
BseMII CTCAG 2 cut(s) 40, 243
BseNI ACTGG 5 cut(s) 229, 319, 328, 354, 936
BseRI GAGGAG 5 cut(s) 60, 145, 148, 151, 187
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 424
BshFI GGCC 2 cut(s) 175, 778
BshNI GGYRCC 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 323
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 2 cut(s) 398, 586
BslI CCNNNNNNNGG 3 cut(s) 171, 806, 858
BsmAI GTCTC 2 cut(s) 83, 248
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 398, 586
BsnI GGCC 2 cut(s) 175, 778
Bso31I GGTCTC 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 2 cut(s) 54, 862
Bsp19I CCATGG 1 cut(s) 551
BspACI CCGC 3 cut(s) 11, 783, 872
BspANI GGCC 2 cut(s) 175, 778
BspCNI CTCAG 2 cut(s) 39, 244
BspLI GGNNCC 2 cut(s) 825, 850
BspPI GGATC 2 cut(s) 49, 870
BspT107I GGYRCC 1 cut(s) 848
BspTNI GGTCTC 1 cut(s) 248
BsrI ACTGG 5 cut(s) 229, 319, 328, 354, 936
BssECI CCNNGG 3 cut(s) 516, 551, 858
BssMI GATC 2 cut(s) 54, 862
BssT1I CCWWGG 1 cut(s) 551
Bst2UI CCWGG 3 cut(s) 172, 780, 859
Bst4CI ACNGT 2 cut(s) 577, 993
Bst6I CTCTTC 1 cut(s) 608
BstC8I GCNNGC 1 cut(s) 595
BstDEI CTNAG 4 cut(s) 26, 46, 201, 252
BstDSI CCRYGG 2 cut(s) 516, 551
BstENI CCTNNNNNAGG 1 cut(s) 804
BstF5I GGATG 3 cut(s) 186, 539, 544
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 2 cut(s) 57, 865
BstMAI GTCTC 2 cut(s) 83, 248
BstMBI GATC 2 cut(s) 54, 862
BstMWI GCNNNNNNNGC 3 cut(s) 473, 878, 887
BstNI CCWGG 3 cut(s) 172, 780, 859
BstNSI RCATGY 1 cut(s) 597
BstSCI CCNGG 3 cut(s) 170, 778, 857
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 862
BstXI CCANNNNNNTGG 1 cut(s) 865
BstYI RGATCY 1 cut(s) 862
BsuRI GGCC 2 cut(s) 175, 778
BtgI CCRYGG 2 cut(s) 516, 551
BtsCI GGATG 3 cut(s) 186, 539, 544
BtsIMutI CAGTG 3 cut(s) 222, 321, 943
Cac8I GCNNGC 1 cut(s) 595
CaiI CAGNNNCTG 1 cut(s) 349
CfoI GCGC 1 cut(s) 515
Cfr13I GGNCC 1 cut(s) 174
Csp6I GTAC 1 cut(s) 957
CviAII CATG 2 cut(s) 552, 594
CviQI GTAC 1 cut(s) 957
DdeI CTNAG 4 cut(s) 26, 46, 201, 252
DpnI GATC 2 cut(s) 56, 864
DpnII GATC 2 cut(s) 54, 862
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
Eco130I CCWWGG 1 cut(s) 551
Eco147I AGGCCT 1 cut(s) 778
Eco31I GGTCTC 1 cut(s) 248
Eco57I CTGAAG 3 cut(s) 285, 710, 924
EcoNI CCTNNNNNAGG 1 cut(s) 804
EcoO109I RGGNCCY 1 cut(s) 174
EcoRII CCWGG 3 cut(s) 170, 778, 857
EcoT14I CCWWGG 1 cut(s) 551
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 551
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 2 cut(s) 555, 597
FaiI YATR 8 cut(s) 363, 531, 553, 595, 698, 741, 758, 961
FaqI GGGAC 2 cut(s) 398, 586
FatI CATG 2 cut(s) 551, 593
FauI CCCGC 1 cut(s) 776
Fnu4HI GCNGC 2 cut(s) 6, 14
FokI GGATG 3 cut(s) 173, 546, 551
Fsp4HI GCNGC 2 cut(s) 6, 14
FspBI CTAG 2 cut(s) 354, 405
GlaI GCGC 1 cut(s) 514
GluI GCNGC 2 cut(s) 6, 14
GsaI CCCAGC 1 cut(s) 428
GsuI CTGGAG 3 cut(s) 396, 841, 864
HaeIII GGCC 2 cut(s) 175, 778
HapII CCGG 1 cut(s) 677
HhaI GCGC 1 cut(s) 515
Hin1II CATG 2 cut(s) 555, 597
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 1 cut(s) 722
HinfI GANTC 4 cut(s) 494, 667, 705, 811
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 1 cut(s) 677
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 3 cut(s) 29, 54, 499
Hpy188III TCNNGA 6 cut(s) 58, 383, 431, 745, 843, 902
Hpy8I GTNNAC 1 cut(s) 580
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 3 cut(s) 45, 203, 550
HpyCH4III ACNGT 2 cut(s) 577, 993
HpyCH4V TGCA 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 878, 887
HpyF3I CTNAG 4 cut(s) 26, 46, 201, 252
Hsp92II CATG 2 cut(s) 555, 597
HspAI GCGC 1 cut(s) 513
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 2 cut(s) 54, 862
LmnI GCTCC 6 cut(s) 13, 55, 318, 553, 650, 823
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 4 cut(s) 580, 662, 917, 934
MaeI CTAG 2 cut(s) 354, 405
MaeIII GTNAC 4 cut(s) 247, 345, 451, 895
MalI GATC 2 cut(s) 56, 864
MboI GATC 2 cut(s) 54, 862
MboII GAAGA 5 cut(s) 30, 301, 304, 401, 625
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 5 cut(s) 333, 435, 483, 565, 736
MlyI GAGTC 1 cut(s) 699
MmeI TCCRAC 1 cut(s) 827
Mph1103I ATGCAT 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 34
MseI TTAA 5 cut(s) 192, 579, 693, 713, 984
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 3 cut(s) 172, 780, 859
MvaI CCWGG 3 cut(s) 172, 780, 859
MwoI GCNNNNNNNGC 3 cut(s) 473, 878, 887
NcoI CCATGG 1 cut(s) 551
NdeII GATC 2 cut(s) 54, 862
NlaIII CATG 2 cut(s) 555, 597
NlaIV GGNNCC 2 cut(s) 825, 850
NmuCI GTSAC 2 cut(s) 451, 895
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 597
PaeI GCATGC 1 cut(s) 597
PceI AGGCCT 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 34
PfeI GAWTC 3 cut(s) 494, 667, 811
PkrI GCNGC 2 cut(s) 7, 15
PleI GAGTC 1 cut(s) 699
PpsI GAGTC 1 cut(s) 699
Psp6I CCWGG 3 cut(s) 170, 778, 857
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 3 cut(s) 170, 778, 857
PspN4I GGNNCC 2 cut(s) 825, 850
PspPI GGNCC 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 349
PsuI RGATCY 1 cut(s) 862
RsaI GTAC 1 cut(s) 958
RsaNI GTAC 1 cut(s) 957
SaqAI TTAA 5 cut(s) 192, 579, 693, 713, 984
SatI GCNGC 2 cut(s) 6, 14
Sau3AI GATC 2 cut(s) 54, 862
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 699
ScrFI CCNGG 3 cut(s) 172, 780, 859
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 4 cut(s) 580, 662, 917, 934
SpeI ACTAGT 1 cut(s) 404
SphI GCATGC 1 cut(s) 597
Sse9I AATT 5 cut(s) 333, 435, 483, 565, 736
SseBI AGGCCT 1 cut(s) 778
SsiI CCGC 3 cut(s) 11, 783, 872
SspMI CTAG 2 cut(s) 354, 405
StuI AGGCCT 1 cut(s) 778
StyD4I CCNGG 3 cut(s) 170, 778, 857
StyI CCWWGG 1 cut(s) 551
TaaI ACNGT 2 cut(s) 577, 993
TaqI TCGA 1 cut(s) 588
TasI AATT 5 cut(s) 333, 435, 483, 565, 736
TfiI GAWTC 3 cut(s) 494, 667, 811
Tru1I TTAA 5 cut(s) 192, 579, 693, 713, 984
Tru9I TTAA 5 cut(s) 192, 579, 693, 713, 984
TscAI CASTG 3 cut(s) 229, 328, 943
TseFI GTSAC 2 cut(s) 451, 895
TseI GCWGC 2 cut(s) 5, 13
Tsp45I GTSAC 2 cut(s) 451, 895
TspDTI ATGAA 2 cut(s) 246, 810
TspGWI ACGGA 2 cut(s) 65, 130
TspRI CASTG 3 cut(s) 229, 328, 943
XagI CCTNNNNNAGG 1 cut(s) 804
XceI RCATGY 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 2 cut(s) 354, 405
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.