Rh5DG101300

protein At2g34460, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008667
Physical Location & Seq
Forward (+)
9251184 .. 9254794
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG101300.1

Sequence Viewer

Length: 1005 bp
ATGGCAGCTCCGCTGCCTCTCATTCTCAGAAACCCTTGTTCTCTCTTAGCTCCGATCCCGACGAAGCCTCCTCTCCGTCTCCCCAACACTCACTCCCACACCCGCCACTACTCTCCTCTCTCCACAACCAAGCCCTTTTCCGTCATTTCACTCTCCTCCTCCTCCACCACCACCACCACCAGACCCTTATTCCCCATCCTCAACTCCTCAAAGATGGAAGGGGGTGAAACCACTGGGAATGAAATCACAGAGGAAGTTACTGAGACCACAACTGAAGCAACAAGTGTGAATGGTTTGAAGAAGATTTTTGTGGCTGGTGCTACTGGGAGCACTGGTAAAAGAATTGTTGAGCAGTTACTGGCTAAGGGTTATGGGGTAAAAGCCGGAGTTCGGGACTTGGAAAAAGCCAAAACTAGTCTTCCAAGCAACAACCCAGCTCTTGAAATTGTGAAAGCAGATGTGACGGAGGGTTCTGCGAAGTTGGCAGAAGCAATTAGTAGTGATTCGGAAGCTGTGATTTGCGCTACGGGGTTTAGCTATGGATGGGATGTGTTTGCTCCATGGAAGGTTGATAATTTTGGGACAGTTAACCTTGTCGATGCATGCCGTAAAGTTGGTGTGAAGAGGTTTATTCTGGTCAGTTCCATTTTAGTCAATGGAGCATCAATGGGGCAGATTCTCAATCCGGCTTACATTTTTCTTAATGTATTTGGACTCACTTTAATCGCAAAGCTTCAGGCAGAGAATTATATCAGGAAGTCTGGTATAAACTACACAATAATAAGGCCTGGTGGGTTGAGAAATGAACCTCCAACAGGGAATCTTGTGATGGAGCCAGAGGACACTCTATCTGGAGGCACCATCTCCAGAGATCTGGTTGCGGAAGTAGCTGTTGAGGCATTGGTCACTCCTGAAGCATCTTACAAGGTAGTTGAGATAGTTTCCAGTGCTGATGCCCCCAAGCGTGCATACAAGGAACTATTTGGTTCGGTTAAGCAACGGTGA

Protein Analysis

334

Amino Acids

35.64

Weight (kDa)

8.82

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 100 - 261 2.5e-18 NmrA-like family
Epimerase PF01370 102 - 263 1.2e-12 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 104 - 226 1.5e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 106 - 303 2.6e-45 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 857
AciI CCGC 3 cut(s) 11, 103, 881
AclWI GGATC 1 cut(s) 49
AcuI CTGAAG 3 cut(s) 294, 719, 933
AfiI CCNNNNNNNGG 2 cut(s) 390, 815
AgsI TTSAA 2 cut(s) 298, 443
AhlI ACTAGT 1 cut(s) 413
AjnI CCWGG 1 cut(s) 787
AluBI AGCT 7 cut(s) 8, 50, 437, 512, 537, 733, 890
AluI AGCT 7 cut(s) 8, 50, 437, 512, 537, 733, 890
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 2 cut(s) 83, 257
AlwI GGATC 1 cut(s) 49
AlwNI CAGNNNCTG 1 cut(s) 358
AoxI GGCC 1 cut(s) 785
ApeKI GCWGC 2 cut(s) 5, 13
AspLEI GCGC 1 cut(s) 524
AsuHPI GGTGA 1 cut(s) 236
BanI GGYRCC 1 cut(s) 857
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 332
BbvI GCAGC 1 cut(s) 17
BccI CCATC 5 cut(s) 203, 208, 537, 823, 869
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 83, 257
BcuI ACTAGT 1 cut(s) 413
BfaI CTAG 1 cut(s) 414
BglII AGATCT 1 cut(s) 871
BisI GCNGC 2 cut(s) 6, 14
BlsI GCNGC 2 cut(s) 7, 15
Bme1390I CCNGG 1 cut(s) 789
BmiI GGNNCC 2 cut(s) 834, 859
BmrFI CCNGG 1 cut(s) 789
BmrI ACTGGG 2 cut(s) 243, 333
BmsI GCATC 4 cut(s) 589, 671, 926, 943
BmuI ACTGGG 2 cut(s) 243, 333
BpiI GAAGAC 1 cut(s) 410
BpmI CTGGAG 2 cut(s) 850, 873
Bpu10I CCTNAGC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 560
BsaXI ACNNNNNCTCC 6 cut(s) 52, 77, 82, 107, 319, 349
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 815
Bse1I ACTGG 5 cut(s) 238, 328, 337, 363, 945
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 1 cut(s) 560
BseGI GGATG 3 cut(s) 195, 548, 553
BseLI CCNNNNNNNGG 2 cut(s) 390, 815
BseMII CTCAG 2 cut(s) 40, 252
BseNI ACTGG 5 cut(s) 238, 328, 337, 363, 945
BseRI GAGGAG 6 cut(s) 60, 105, 145, 148, 151, 196
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 433
BshFI GGCC 1 cut(s) 787
BshNI GGYRCC 1 cut(s) 857
BsiHKAI GWGCWC 1 cut(s) 332
BsiSI CCGG 2 cut(s) 384, 686
BslFI GGGAC 2 cut(s) 407, 595
BslI CCNNNNNNNGG 2 cut(s) 390, 815
BsmAI GTCTC 2 cut(s) 83, 257
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 407, 595
BsnI GGCC 1 cut(s) 787
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 2 cut(s) 54, 871
Bsp19I CCATGG 1 cut(s) 560
BspACI CCGC 3 cut(s) 11, 103, 881
BspANI GGCC 1 cut(s) 787
BspCNI CTCAG 2 cut(s) 39, 253
BspLI GGNNCC 2 cut(s) 834, 859
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 857
BspTNI GGTCTC 1 cut(s) 257
BsrI ACTGG 5 cut(s) 238, 328, 337, 363, 945
BssECI CCNNGG 1 cut(s) 560
BssMI GATC 2 cut(s) 54, 871
BssT1I CCWWGG 1 cut(s) 560
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 2 cut(s) 586, 1002
Bst6I CTCTTC 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 604, 966
BstDEI CTNAG 4 cut(s) 26, 46, 261, 363
BstDSI CCRYGG 1 cut(s) 560
BstENI CCTNNNNNAGG 1 cut(s) 813
BstF5I GGATG 3 cut(s) 195, 548, 553
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 2 cut(s) 57, 874
BstMAI GTCTC 2 cut(s) 83, 257
BstMBI GATC 2 cut(s) 54, 871
BstMWI GCNNNNNNNGC 3 cut(s) 482, 887, 896
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 1 cut(s) 606
BstSCI CCNGG 1 cut(s) 787
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 410
BstX2I RGATCY 1 cut(s) 871
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 1 cut(s) 871
BsuRI GGCC 1 cut(s) 787
BtgI CCRYGG 1 cut(s) 560
BtsCI GGATG 3 cut(s) 195, 548, 553
BtsIMutI CAGTG 3 cut(s) 231, 330, 952
Cac8I GCNNGC 2 cut(s) 604, 966
CaiI CAGNNNCTG 1 cut(s) 358
CfoI GCGC 1 cut(s) 524
CviAII CATG 2 cut(s) 561, 603
DdeI CTNAG 4 cut(s) 26, 46, 261, 363
DpnI GATC 2 cut(s) 56, 873
DpnII GATC 2 cut(s) 54, 871
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 560
Eco147I AGGCCT 1 cut(s) 787
Eco31I GGTCTC 1 cut(s) 257
Eco57I CTGAAG 3 cut(s) 294, 719, 933
EcoNI CCTNNNNNAGG 1 cut(s) 813
EcoRII CCWGG 1 cut(s) 787
EcoT14I CCWWGG 1 cut(s) 560
EcoT22I ATGCAT 1 cut(s) 604
ErhI CCWWGG 1 cut(s) 560
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 2 cut(s) 564, 606
FaiI YATR 7 cut(s) 372, 540, 562, 604, 750, 767, 970
FaqI GGGAC 2 cut(s) 407, 595
FatI CATG 2 cut(s) 560, 602
FauI CCCGC 1 cut(s) 110
Fnu4HI GCNGC 2 cut(s) 6, 14
FokI GGATG 3 cut(s) 182, 555, 560
Fsp4HI GCNGC 2 cut(s) 6, 14
FspBI CTAG 1 cut(s) 414
GlaI GCGC 1 cut(s) 523
GluI GCNGC 2 cut(s) 6, 14
GsaI CCCAGC 1 cut(s) 437
GsuI CTGGAG 2 cut(s) 850, 873
HaeIII GGCC 1 cut(s) 787
HapII CCGG 2 cut(s) 384, 686
HhaI GCGC 1 cut(s) 524
Hin1II CATG 2 cut(s) 564, 606
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 4 cut(s) 503, 676, 714, 820
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 2 cut(s) 384, 686
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 3 cut(s) 29, 54, 508
Hpy188III TCNNGA 7 cut(s) 58, 392, 440, 754, 852, 867, 911
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 212, 559
HpyCH4III ACNGT 2 cut(s) 586, 1002
HpyCH4V TGCA 2 cut(s) 602, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 482, 887, 896
HpyF3I CTNAG 4 cut(s) 26, 46, 261, 363
Hsp92II CATG 2 cut(s) 564, 606
HspAI GCGC 1 cut(s) 522
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 2 cut(s) 54, 871
LmnI GCTCC 6 cut(s) 13, 55, 327, 562, 659, 832
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 4 cut(s) 589, 671, 926, 943
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 4 cut(s) 256, 354, 460, 904
MalI GATC 2 cut(s) 56, 873
MboI GATC 2 cut(s) 54, 871
MboII GAAGA 4 cut(s) 310, 313, 410, 634
MflI RGATCY 1 cut(s) 871
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 5 cut(s) 342, 444, 492, 574, 745
MlyI GAGTC 1 cut(s) 708
MmeI TCCRAC 1 cut(s) 836
Mph1103I ATGCAT 1 cut(s) 604
MseI TTAA 4 cut(s) 588, 702, 722, 993
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 2 cut(s) 384, 686
MspR9I CCNGG 1 cut(s) 789
MvaI CCWGG 1 cut(s) 789
MwoI GCNNNNNNNGC 3 cut(s) 482, 887, 896
NcoI CCATGG 1 cut(s) 560
NdeII GATC 2 cut(s) 54, 871
NlaIII CATG 2 cut(s) 564, 606
NlaIV GGNNCC 2 cut(s) 834, 859
NmuCI GTSAC 2 cut(s) 460, 904
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 1 cut(s) 606
PaeI GCATGC 1 cut(s) 606
PceI AGGCCT 1 cut(s) 787
PfeI GAWTC 3 cut(s) 503, 676, 820
PkrI GCNGC 2 cut(s) 7, 15
PleI GAGTC 1 cut(s) 708
PpsI GAGTC 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 787
PspFI CCCAGC 1 cut(s) 433
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 2 cut(s) 834, 859
PstNI CAGNNNCTG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 871
SaqAI TTAA 4 cut(s) 588, 702, 722, 993
SatI GCNGC 2 cut(s) 6, 14
Sau3AI GATC 2 cut(s) 54, 871
SchI GAGTC 1 cut(s) 708
ScrFI CCNGG 1 cut(s) 789
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 4 cut(s) 589, 671, 926, 943
SpeI ACTAGT 1 cut(s) 413
SphI GCATGC 1 cut(s) 606
Sse9I AATT 5 cut(s) 342, 444, 492, 574, 745
SseBI AGGCCT 1 cut(s) 787
SsiI CCGC 3 cut(s) 11, 103, 881
SspMI CTAG 1 cut(s) 414
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 1 cut(s) 787
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 2 cut(s) 586, 1002
TaqI TCGA 1 cut(s) 597
TasI AATT 5 cut(s) 342, 444, 492, 574, 745
TfiI GAWTC 3 cut(s) 503, 676, 820
Tru1I TTAA 4 cut(s) 588, 702, 722, 993
Tru9I TTAA 4 cut(s) 588, 702, 722, 993
TscAI CASTG 3 cut(s) 238, 337, 952
TseFI GTSAC 2 cut(s) 460, 904
TseI GCWGC 2 cut(s) 5, 13
Tsp45I GTSAC 2 cut(s) 460, 904
TspDTI ATGAA 2 cut(s) 255, 819
TspGWI ACGGA 3 cut(s) 65, 130, 479
TspRI CASTG 3 cut(s) 238, 337, 952
XagI CCTNNNNNAGG 1 cut(s) 813
XceI RCATGY 1 cut(s) 606
XspI CTAG 1 cut(s) 414
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.