Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008667

Physical Location & Seq

Genomic Coordinates Reverse (-)

41931756 .. 41934365

Gene Structure 2610 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1286400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 936 bp

ATGGCAACTCCTCTGCCTCTCCTTCTCAGAAACCCTCCTCACCGTCTCTGCACTCACCGCCTCCTCCCTCCTCTTTCCACCACCACCATCAAACCCTTCTCTCTGGTTTCCTTCTCCTCCTCCAACACCAGGTCCTTTCCCATCCTAAAGTCCGCTCAGATGGAAGGGGGTGAAATTACAGAGGAAGTTACAGAGATAAGTTCAACTGAGACCAATGTGATTGGGAAGAAGAAAATATTTGTGGCTGGTGCCACTGGAAATACTGGTAAAAGAATTGTTGAGCAGCTGCTGGCCAAGGGTTTCTCAGTTAAAGCCGGAGTTCGGAACTTGGAGAAAGCGAAAGCTAGCCTTCCCTCAGACAACCCAGCTCTTCAAATTGTGAAGGCAGATGTGACAGATGGTTCTGCTAAGTTAGCAGAAGTGATAAGTGATGATTCAGAAGCTGTGATTTGTGCCACTGGATTTAGCTACGGATGGGATGTGTTTGCTCCATGGAAGGTTGATAATTTTGGCACAGTAAACCTTGTTGAAGCATGCCGTAAACTTGGTGTGAACCGGTTTATTCTTGTGAGTTCCATCTTGGTCAATGGAGCAGCAATGGGGCAGATTCTCAACCCAGCTTACATCTTCCTCAATGTTTTTGGGCTCACCTTAATAGCAAAGCTTCAGGCAGAGAATTATATCAGGAAGTCTGGCATAAACTACACGATTATAAGGCCCGGTGGGTTGAGAAACGACCCTCCAACCGGAAATCTTGTAATGGAGCCAGAGGACACTCTTTCGGAAGGCAGCATCTCTAGAGACCTGGTTGCAGAAGTGGCTGTAGAGGCATTACGGAATCCTGAAGCGTCTTACAAGGTTGTGGAGATAGTTTCGCGGCCTGATGCCCCTAAGCGTTCTTATGAGGATCTTTTCGGTTCCATCAAGCAACGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0010287 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAD_binding_10

Protein Analysis

312

Amino Acids

33.65

Weight (kDa)

8.66

Isoelectric Point (pI)

38.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	76 - 238	3.5e-17	NmrA-like family
Epimerase	PF01370	79 - 240	1.1e-12	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	80 - 193	5.1e-06	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	81 - 202	3.7e-08	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10	PF13460	83 - 281	3.4e-46	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008667)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34460
fragaria_vesca	FvH4_3g05850 FvH4_3g05850 FvH4_3g05850
malus_domestica	MD05G1286400.v1.1 MD05G1286500.v1.1 MD10G1264000.v1.1
prunus_persica	Prupe.4G078400_v2.0.a1 Prupe.4G078500_v2.0.a1
pyrus_communis	pycom05g26490 pycom10g22010
rosa_chinensis	RchiOBHm_Chr5g0013671 RchiOBHm_Chr6g0254821
rosa_laevigata	RLG00000009678 RLG00000032039
rosa_roxburghii	Rroxscaffold_1G00062930
rosa_rugosa	Rorug05G0011900 Rorug05G0012000
rosa_samantha	Rh5AG106400 Rh5BG102600 Rh5CG114500 Rh5DG101300
rosa_wichuraiana	Rw5G009280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	713
AccB1I	GGYRCC	1 cut(s)	248
AccBSI	CCGCTC	1 cut(s)	155
AccII	CGCG	1 cut(s)	877
AciI	CCGC	3 cut(s)	58, 153, 877
AclWI	GGATC	1 cut(s)	915
AcoI	YGGCCR	1 cut(s)	291
AcuI	CTGAAG	2 cut(s)	650, 864
AfiI	CCNNNNNNNGG	3 cut(s)	129, 321, 746
AgeI	ACCGGT	1 cut(s)	555
AgsI	TTSAA	3 cut(s)	204, 374, 530
AjnI	CCWGG	2 cut(s)	128, 804
AluBI	AGCT	7 cut(s)	286, 344, 368, 443, 468, 620, 664
AluI	AGCT	7 cut(s)	286, 344, 368, 443, 468, 620, 664
Alw26I	GTCTC	3 cut(s)	50, 203, 795
AlwI	GGATC	1 cut(s)	915
AlwNI	CAGNNNCTG	2 cut(s)	289, 443
AoxI	GGCC	3 cut(s)	291, 716, 878
ApeKI	GCWGC	4 cut(s)	283, 286, 593, 789
AsiGI	ACCGGT	1 cut(s)	555
AspS9I	GGNCC	2 cut(s)	132, 717
AsuC2I	CCSGG	1 cut(s)	720
AsuHPI	GGTGA	4 cut(s)	32, 47, 182, 640
AsuNHI	GCTAGC	1 cut(s)	344
AvaII	GGWCC	1 cut(s)	132
BalI	TGGCCA	1 cut(s)	293
BanI	GGYRCC	1 cut(s)	248
BanII	GRGCYC	1 cut(s)	648
BbvI	GCAGC	4 cut(s)	273, 295, 605, 801
BccI	CCATC	7 cut(s)	95, 149, 154, 392, 468, 584, 929
BceAI	ACGGC	1 cut(s)	522
BciT130I	CCWGG	2 cut(s)	130, 806
BcnI	CCSGG	1 cut(s)	720
BcoDI	GTCTC	3 cut(s)	50, 203, 795
BfaI	CTAG	2 cut(s)	345, 798
BfmI	CTRYAG	1 cut(s)	822
BisI	GCNGC	5 cut(s)	284, 287, 594, 790, 878
BlsI	GCNGC	5 cut(s)	285, 288, 595, 791, 879
Bme1390I	CCNGG	3 cut(s)	130, 720, 806
Bme18I	GGWCC	1 cut(s)	132
BmgT120I	GGNCC	2 cut(s)	132, 717
BmiI	GGNNCC	3 cut(s)	250, 765, 919
BmrFI	CCNGG	3 cut(s)	130, 720, 806
BmsI	GCATC	2 cut(s)	801, 874
BmtI	GCTAGC	1 cut(s)	348
Bpu10I	CCTNAGC	1 cut(s)	891
BpuMI	CCSGG	1 cut(s)	720
BsaI	GGTCTC	2 cut(s)	203, 795
BsaJI	CCNNGG	2 cut(s)	294, 491
BsaWI	WCCGGW	2 cut(s)	555, 746
Bsc4I	CCNNNNNNNGG	3 cut(s)	129, 321, 746
Bse118I	RCCGGY	1 cut(s)	555
Bse1I	ACTGG	3 cut(s)	259, 268, 463
Bse3DI	GCAATG	1 cut(s)	603
BseBI	CCWGG	2 cut(s)	130, 806
BseDI	CCNNGG	2 cut(s)	294, 491
BseGI	GGATG	3 cut(s)	141, 479, 484
BseLI	CCNNNNNNNGG	3 cut(s)	129, 321, 746
BseMI	GCAATG	1 cut(s)	603
BseMII	CTCAG	5 cut(s)	40, 170, 198, 318, 369
BseNI	ACTGG	3 cut(s)	259, 268, 463
BseRI	GAGGAG	5 cut(s)	27, 53, 60, 106, 109
BseXI	GCAGC	4 cut(s)	273, 295, 605, 801
BseYI	CCCAGC	2 cut(s)	364, 616
BsgI	GTGCAG	1 cut(s)	34
Bsh1236I	CGCG	1 cut(s)	877
BshFI	GGCC	3 cut(s)	293, 718, 880
BshNI	GGYRCC	1 cut(s)	248
BshTI	ACCGGT	1 cut(s)	555
BsiSI	CCGG	4 cut(s)	315, 556, 720, 747
BslI	CCNNNNNNNGG	3 cut(s)	129, 321, 746
BsmAI	GTCTC	3 cut(s)	50, 203, 795
BsmBI	CGTCTC	1 cut(s)	50
BsnI	GGCC	3 cut(s)	293, 718, 880
Bso31I	GGTCTC	2 cut(s)	203, 795
Bsp1286I	GDGCHC	1 cut(s)	648
Bsp143I	GATC	1 cut(s)	907
Bsp19I	CCATGG	1 cut(s)	491
BspACI	CCGC	3 cut(s)	58, 153, 877
BspANI	GGCC	3 cut(s)	293, 718, 880
BspCNI	CTCAG	5 cut(s)	39, 169, 199, 317, 368
BspFNI	CGCG	1 cut(s)	877
BspLI	GGNNCC	3 cut(s)	250, 765, 919
BspOI	GCTAGC	1 cut(s)	348
BspPI	GGATC	1 cut(s)	915
BspQI	GCTCTTC	1 cut(s)	375
BspT107I	GGYRCC	1 cut(s)	248
BspTNI	GGTCTC	2 cut(s)	203, 795
BsrBI	CCGCTC	1 cut(s)	155
BsrDI	GCAATG	1 cut(s)	603
BsrFI	RCCGGY	1 cut(s)	555
BsrI	ACTGG	3 cut(s)	259, 268, 463
BssAI	RCCGGY	1 cut(s)	555
BssECI	CCNNGG	2 cut(s)	294, 491
BssMI	GATC	1 cut(s)	907
BssT1I	CCWWGG	2 cut(s)	294, 491
Bst2UI	CCWGG	2 cut(s)	130, 806
Bst4CI	ACNGT	3 cut(s)	44, 517, 933
Bst6I	CTCTTC	1 cut(s)	375
BstC8I	GCNNGC	3 cut(s)	291, 346, 535
BstDEI	CTNAG	7 cut(s)	26, 156, 207, 304, 355, 408, 891
BstDSI	CCRYGG	1 cut(s)	491
BstF5I	GGATG	3 cut(s)	141, 479, 484
BstFNI	CGCG	1 cut(s)	877
BstKTI	GATC	1 cut(s)	910
BstMAI	GTCTC	3 cut(s)	50, 203, 795
BstMBI	GATC	1 cut(s)	907
BstMWI	GCNNNNNNNGC	4 cut(s)	57, 413, 818, 827
BstNI	CCWGG	2 cut(s)	130, 806
BstNSI	RCATGY	1 cut(s)	537
BstSCI	CCNGG	3 cut(s)	128, 718, 804
BstSFI	CTRYAG	1 cut(s)	822
BstUI	CGCG	1 cut(s)	877
BstV1I	GCAGC	4 cut(s)	273, 295, 605, 801
BstX2I	RGATCY	1 cut(s)	907
BstYI	RGATCY	1 cut(s)	907
BsuRI	GGCC	3 cut(s)	293, 718, 880
BtgI	CCRYGG	1 cut(s)	491
BtsCI	GGATG	3 cut(s)	141, 479, 484
BtsIMutI	CAGTG	2 cut(s)	252, 456
Cac8I	GCNNGC	3 cut(s)	291, 346, 535
CaiI	CAGNNNCTG	2 cut(s)	289, 443
Cfr10I	RCCGGY	1 cut(s)	555
Cfr13I	GGNCC	2 cut(s)	132, 717
CseI	GACGC	1 cut(s)	837
CsiI	ACCWGGT	2 cut(s)	128, 804
CspAI	ACCGGT	1 cut(s)	555
CviAII	CATG	2 cut(s)	492, 534
DdeI	CTNAG	7 cut(s)	26, 156, 207, 304, 355, 408, 891
DpnI	GATC	1 cut(s)	909
DpnII	GATC	1 cut(s)	907
EaeI	YGGCCR	1 cut(s)	291
Eam1104I	CTCTTC	1 cut(s)	375
EarI	CTCTTC	1 cut(s)	375
Eco130I	CCWWGG	2 cut(s)	294, 491
Eco24I	GRGCYC	1 cut(s)	648
Eco31I	GGTCTC	2 cut(s)	203, 795
Eco47I	GGWCC	1 cut(s)	132
Eco57I	CTGAAG	2 cut(s)	650, 864
EcoO109I	RGGNCCY	1 cut(s)	132
EcoRII	CCWGG	2 cut(s)	128, 804
EcoT14I	CCWWGG	2 cut(s)	294, 491
EcoT38I	GRGCYC	1 cut(s)	648
ErhI	CCWWGG	2 cut(s)	294, 491
Esp3I	CGTCTC	1 cut(s)	50
FaeI	CATG	2 cut(s)	495, 537
FaiI	YATR	6 cut(s)	493, 535, 681, 698, 713, 903
FalI	AAGNNNNNCTT	2 cut(s)	333, 365
FatI	CATG	2 cut(s)	491, 533
Fnu4HI	GCNGC	5 cut(s)	284, 287, 594, 790, 878
FokI	GGATG	3 cut(s)	128, 486, 491
FriOI	GRGCYC	1 cut(s)	648
Fsp4HI	GCNGC	5 cut(s)	284, 287, 594, 790, 878
FspBI	CTAG	2 cut(s)	345, 798
GluI	GCNGC	5 cut(s)	284, 287, 594, 790, 878
GsaI	CCCAGC	2 cut(s)	368, 620
HaeIII	GGCC	3 cut(s)	293, 718, 880
HapII	CCGG	4 cut(s)	315, 556, 720, 747
HgaI	GACGC	1 cut(s)	837
Hin1II	CATG	2 cut(s)	495, 537
HindIII	AAGCTT	1 cut(s)	662
HinfI	GANTC	3 cut(s)	434, 607, 838
HpaII	CCGG	4 cut(s)	315, 556, 720, 747
HphI	GGTGA	4 cut(s)	32, 47, 182, 640
Hpy166II	GTNNAC	3 cut(s)	520, 542, 553
Hpy188I	TCNGA	6 cut(s)	29, 159, 324, 358, 439, 784
Hpy188III	TCNNGA	3 cut(s)	685, 798, 842
Hpy8I	GTNNAC	3 cut(s)	520, 542, 553
HpyAV	CCTTC	8 cut(s)	32, 106, 121, 158, 359, 376, 490, 779
HpyCH4III	ACNGT	3 cut(s)	44, 517, 933
HpyCH4V	TGCA	2 cut(s)	51, 812
HpyF10VI	GCNNNNNNNGC	4 cut(s)	57, 413, 818, 827
HpyF3I	CTNAG	7 cut(s)	26, 156, 207, 304, 355, 408, 891
Hsp92II	CATG	2 cut(s)	495, 537
Kzo9I	GATC	1 cut(s)	907
LguI	GCTCTTC	1 cut(s)	375
LmnI	GCTCC	3 cut(s)	493, 590, 763
Lsp1109I	GCAGC	4 cut(s)	273, 295, 605, 801
LweI	GCATC	2 cut(s)	801, 874
MabI	ACCWGGT	2 cut(s)	128, 804
MaeI	CTAG	2 cut(s)	345, 798
MaeIII	GTNAC	2 cut(s)	187, 391
MalI	GATC	1 cut(s)	909
MbiI	CCGCTC	1 cut(s)	155
MboI	GATC	1 cut(s)	907
MboII	GAAGA	4 cut(s)	238, 241, 362, 619
MflI	RGATCY	1 cut(s)	907
MhlI	GDGCHC	1 cut(s)	648
MlsI	TGGCCA	1 cut(s)	293
MluCI	AATT	5 cut(s)	174, 273, 375, 505, 676
MluNI	TGGCCA	1 cut(s)	293
MmeI	TCCRAC	2 cut(s)	147, 767
Mox20I	TGGCCA	1 cut(s)	293
MscI	TGGCCA	1 cut(s)	293
MseI	TTAA	2 cut(s)	309, 653
Msp20I	TGGCCA	1 cut(s)	293
MspA1I	CMGCKG	1 cut(s)	286
MspI	CCGG	4 cut(s)	315, 556, 720, 747
MspR9I	CCNGG	3 cut(s)	130, 720, 806
MvaI	CCWGG	2 cut(s)	130, 806
MvnI	CGCG	1 cut(s)	877
MwoI	GCNNNNNNNGC	4 cut(s)	57, 413, 818, 827
NciI	CCSGG	1 cut(s)	720
NcoI	CCATGG	1 cut(s)	491
NdeII	GATC	1 cut(s)	907
NheI	GCTAGC	1 cut(s)	344
NlaIII	CATG	2 cut(s)	495, 537
NlaIV	GGNNCC	3 cut(s)	250, 765, 919
NmuCI	GTSAC	1 cut(s)	391
NspI	RCATGY	1 cut(s)	537
PaeI	GCATGC	1 cut(s)	537
PciSI	GCTCTTC	1 cut(s)	375
PfeI	GAWTC	3 cut(s)	434, 607, 838
PinAI	ACCGGT	1 cut(s)	555
PkrI	GCNGC	5 cut(s)	285, 288, 595, 791, 879
PpuMI	RGGWCCY	1 cut(s)	132
PsiI	TTATAA	1 cut(s)	713
Psp5II	RGGWCCY	1 cut(s)	132
Psp6I	CCWGG	2 cut(s)	128, 804
PspFI	CCCAGC	2 cut(s)	364, 616
PspGI	CCWGG	2 cut(s)	128, 804
PspN4I	GGNNCC	3 cut(s)	250, 765, 919
PspPI	GGNCC	2 cut(s)	132, 717
PspPPI	RGGWCCY	1 cut(s)	132
PstNI	CAGNNNCTG	2 cut(s)	289, 443
PsuI	RGATCY	1 cut(s)	907
PvuII	CAGCTG	1 cut(s)	286
SapI	GCTCTTC	1 cut(s)	375
SaqAI	TTAA	2 cut(s)	309, 653
SatI	GCNGC	5 cut(s)	284, 287, 594, 790, 878
Sau3AI	GATC	1 cut(s)	907
Sau96I	GGNCC	2 cut(s)	132, 717
ScrFI	CCNGG	3 cut(s)	130, 720, 806
SduI	GDGCHC	1 cut(s)	648
SexAI	ACCWGGT	2 cut(s)	128, 804
SfaNI	GCATC	2 cut(s)	801, 874
SfcI	CTRYAG	1 cut(s)	822
SinI	GGWCC	1 cut(s)	132
SphI	GCATGC	1 cut(s)	537
Sse9I	AATT	5 cut(s)	174, 273, 375, 505, 676
SsiI	CCGC	3 cut(s)	58, 153, 877
SspI	AATATT	1 cut(s)	237
SspMI	CTAG	2 cut(s)	345, 798
StyD4I	CCNGG	3 cut(s)	128, 718, 804
StyI	CCWWGG	2 cut(s)	294, 491
TaaI	ACNGT	3 cut(s)	44, 517, 933
TasI	AATT	5 cut(s)	174, 273, 375, 505, 676
TauI	GCSGC	1 cut(s)	880
TfiI	GAWTC	3 cut(s)	434, 607, 838
Tru1I	TTAA	2 cut(s)	309, 653
Tru9I	TTAA	2 cut(s)	309, 653
TscAI	CASTG	2 cut(s)	259, 463
TseFI	GTSAC	1 cut(s)	391
TseI	GCWGC	4 cut(s)	283, 286, 593, 789
Tsp45I	GTSAC	1 cut(s)	391
TspGWI	ACGGA	2 cut(s)	486, 850
TspRI	CASTG	2 cut(s)	259, 463
VpaK11BI	GGWCC	1 cut(s)	132
XbaI	TCTAGA	1 cut(s)	797
XceI	RCATGY	1 cut(s)	537
XspI	CTAG	2 cut(s)	345, 798

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.