AT2G36680

endosome transport via multivesicular body sorting pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008384
Physical Location & Seq
Reverse (-)
15368792 .. 15371033
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G36680.4

Sequence Viewer

Length: 657 bp
ATGTTCAATTTCTGGGGATCAAAAGAACAGCAACAAGGACAATCTCGTCCTTCTCCAGAAGCTTCTGCAACACCGTGGTATTCGCCGTCTCTTGTCACCTCTCCTAGCTCATCCCGGCCTCAAACCTCAGGGCAGATTCCATCACATGTTTCACCAGGTGAAGCCGCCGGCATTATTGCCATTTTAAAAGACAAAAGTGTGGATGAGCTTAGGAAGCTTCTCTCTGACAAGGATGCGTATCAACAGTTTCTGCACTCGCTTGACCAGGTCACTATTCAAAACAATATCAGAGAAGAGCTTCGCAAAGAAACGTTACATCTAGCTAGAGAAAACTTGGAGAAGGAACCACAGATAGTGGAGCTCAGAAACCAATGCAGAATAATCCGTACATCCGAGCTTGCGACTGCTCAAGAGAAGCTCAATGAGCTTGAGAATCAAAGAGAAGAGATCCTCAAATTCTACTCTCCAGGTTCTCTTCTTCATAGACTTCAAGATGCTATGAACCAGGTGGATGAAGAATCAGAGGAGCTGCAACAGAAGTTTATGGAGAAGGATATTGACACAGCTGCATTTGTGCAGAAATACAAAAAGCTAAGAAGCAAGTATCATCGACGGGCTTTGATTCATCTCGCTGCTAAAACTTCATCTATTGGCTGA

Protein Analysis

218

Amino Acids

24.99

Weight (kDa)

6.61

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mod_r PF07200 63 - 138 2.9e-17 Modifier of rudimentary (Mod(r)) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 45
AciI CCGC 1 cut(s) 165
AclI AACGTT 1 cut(s) 311
AclWI GGATC 2 cut(s) 25, 442
AcsI RAATTY 1 cut(s) 455
AdeI CACNNNGTG 1 cut(s) 158
AfaI GTAC 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 3 cut(s) 7, 278, 491
AjnI CCWGG 4 cut(s) 154, 264, 466, 504
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 2 cut(s) 25, 442
AlwNI CAGNNNCTG 1 cut(s) 250
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 3 cut(s) 529, 566, 632
ApoI RAATTY 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 297
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 3 cut(s) 88, 144, 170
AxyI CCTNAGG 1 cut(s) 127
BanII GRGCYC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 516, 553, 619
BccI CCATC 1 cut(s) 148
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 4 cut(s) 156, 266, 468, 506
BcnI CCSGG 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 3 cut(s) 105, 320, 324
BisI GCNGC 4 cut(s) 165, 530, 567, 633
BlsI GCNGC 4 cut(s) 166, 531, 568, 634
Bme1390I CCNGG 5 cut(s) 115, 156, 266, 468, 506
BmiI GGNNCC 1 cut(s) 345
BmrFI CCNGG 5 cut(s) 115, 156, 266, 468, 506
BmsI GCATC 2 cut(s) 223, 484
BpmI CTGGAG 2 cut(s) 39, 450
Bpu10I CCTNAGC 1 cut(s) 209
BpuEI CTTGAG 2 cut(s) 393, 449
BpuMI CCSGG 1 cut(s) 115
BsaBI GATNNNNATC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 74
Bse118I RCCGGY 1 cut(s) 167
Bse21I CCTNAGG 1 cut(s) 127
Bse8I GATNNNNATC 1 cut(s) 237
BseBI CCWGG 4 cut(s) 156, 266, 468, 506
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 5 cut(s) 110, 208, 238, 389, 517
BseJI GATNNNNATC 1 cut(s) 237
BseMII CTCAG 2 cut(s) 141, 376
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 3 cut(s) 516, 553, 619
BsgI GTGCAG 2 cut(s) 236, 596
BshFI GGCC 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 2 cut(s) 115, 168
BsmAI GTCTC 1 cut(s) 93
BsmBI CGTCTC 1 cut(s) 93
BsnI GGCC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 2 cut(s) 17, 447
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 2 cut(s) 140, 375
BspLI GGNNCC 1 cut(s) 345
BspPI GGATC 2 cut(s) 25, 442
BspQI GCTCTTC 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 2 cut(s) 17, 447
Bst2UI CCWGG 4 cut(s) 156, 266, 468, 506
Bst4CI ACNGT 2 cut(s) 75, 246
Bst6I CTCTTC 3 cut(s) 288, 438, 480
BstC8I GCNNGC 2 cut(s) 169, 399
BstDEI CTNAG 4 cut(s) 127, 209, 362, 593
BstDSI CCRYGG 1 cut(s) 74
BstF5I GGATG 5 cut(s) 110, 208, 238, 389, 517
BstKTI GATC 2 cut(s) 20, 450
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 2 cut(s) 17, 447
BstMWI GCNNNNNNNGC 2 cut(s) 214, 424
BstNI CCWGG 4 cut(s) 156, 266, 468, 506
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 5 cut(s) 113, 154, 264, 466, 504
BstV1I GCAGC 3 cut(s) 516, 553, 619
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
Bsu36I CCTNAGG 1 cut(s) 127
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 74
BtsCI GGATG 5 cut(s) 110, 208, 238, 389, 517
Cac8I GCNNGC 2 cut(s) 169, 399
CaiI CAGNNNCTG 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 167
CsiI ACCWGGT 3 cut(s) 154, 264, 504
Csp6I GTAC 1 cut(s) 387
CviAII CATG 1 cut(s) 146
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 4 cut(s) 127, 209, 362, 593
DpnI GATC 2 cut(s) 19, 449
DpnII GATC 2 cut(s) 17, 447
DraI TTTAAA 1 cut(s) 186
DraIII CACNNNGTG 1 cut(s) 158
DrdI GACNNNNNNGTC 1 cut(s) 45
DseDI GACNNNNNNGTC 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 288, 438, 480
EarI CTCTTC 3 cut(s) 288, 438, 480
Ecl136II GAGCTC 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 361
Eco81I CCTNAGG 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 361
EcoRII CCWGG 4 cut(s) 154, 264, 466, 504
EcoT38I GRGCYC 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 1 cut(s) 149
FaiI YATR 4 cut(s) 147, 483, 500, 545
FatI CATG 1 cut(s) 145
Fnu4HI GCNGC 4 cut(s) 165, 530, 567, 633
FokI GGATG 5 cut(s) 97, 215, 245, 376, 524
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 4 cut(s) 165, 530, 567, 633
FspBI CTAG 3 cut(s) 105, 320, 324
GluI GCNGC 4 cut(s) 165, 530, 567, 633
GsuI CTGGAG 2 cut(s) 39, 450
HaeIII GGCC 1 cut(s) 118
HapII CCGG 2 cut(s) 115, 168
Hin1II CATG 1 cut(s) 149
HindIII AAGCTT 2 cut(s) 60, 215
HinfI GANTC 4 cut(s) 136, 433, 518, 622
HpaII CCGG 2 cut(s) 115, 168
HphI GGTGA 3 cut(s) 88, 144, 170
Hpy188I TCNGA 5 cut(s) 226, 290, 365, 394, 523
Hpy188III TCNNGA 3 cut(s) 56, 410, 491
Hpy99I CGWCG 1 cut(s) 615
HpyAV CCTTC 3 cut(s) 60, 334, 544
HpyCH4III ACNGT 2 cut(s) 75, 246
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 6 cut(s) 68, 253, 375, 532, 569, 577
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 424
HpyF3I CTNAG 4 cut(s) 127, 209, 362, 593
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 1 cut(s) 149
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 17, 447
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 358, 526
Lsp1109I GCAGC 3 cut(s) 516, 553, 619
LweI GCATC 2 cut(s) 223, 484
MabI ACCWGGT 3 cut(s) 154, 264, 504
MaeI CTAG 3 cut(s) 105, 320, 324
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 3 cut(s) 94, 268, 312
MalI GATC 2 cut(s) 19, 449
MboI GATC 2 cut(s) 17, 447
MboII GAAGA 5 cut(s) 305, 455, 467, 470, 527
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 2 cut(s) 7, 455
MnlI CCTC 5 cut(s) 109, 129, 136, 461, 517
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 2 cut(s) 115, 168
MspR9I CCNGG 5 cut(s) 115, 156, 266, 468, 506
MvaI CCWGG 4 cut(s) 156, 266, 468, 506
MwoI GCNNNNNNNGC 2 cut(s) 214, 424
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 1 cut(s) 115
NdeII GATC 2 cut(s) 17, 447
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 149
NlaIV GGNNCC 1 cut(s) 345
NmuCI GTSAC 2 cut(s) 94, 268
NspI RCATGY 1 cut(s) 149
PciI ACATGT 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 288
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 4 cut(s) 136, 433, 518, 622
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 4 cut(s) 166, 531, 568, 634
PscI ACATGT 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 363
Psp1406I AACGTT 1 cut(s) 311
Psp6I CCWGG 4 cut(s) 154, 264, 466, 504
PspGI CCWGG 4 cut(s) 154, 264, 466, 504
PspN4I GGNNCC 1 cut(s) 345
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 1 cut(s) 447
PsyI GACNNNGTC 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SacI GAGCTC 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 185
SatI GCNGC 4 cut(s) 165, 530, 567, 633
Sau3AI GATC 2 cut(s) 17, 447
ScrFI CCNGG 5 cut(s) 115, 156, 266, 468, 506
SduI GDGCHC 1 cut(s) 363
SexAI ACCWGGT 3 cut(s) 154, 264, 504
SfaNI GCATC 2 cut(s) 223, 484
SmlI CTYRAG 2 cut(s) 408, 428
SmoI CTYRAG 2 cut(s) 408, 428
Sse9I AATT 2 cut(s) 7, 455
SsiI CCGC 1 cut(s) 165
SspMI CTAG 3 cut(s) 105, 320, 324
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 5 cut(s) 113, 154, 264, 466, 504
TaaI ACNGT 2 cut(s) 75, 246
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 610
TasI AATT 2 cut(s) 7, 455
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 4 cut(s) 136, 433, 518, 622
Tru1I TTAA 1 cut(s) 185
Tru9I TTAA 1 cut(s) 185
TseFI GTSAC 2 cut(s) 94, 268
TseI GCWGC 3 cut(s) 529, 566, 632
Tsp45I GTSAC 2 cut(s) 94, 268
TspDTI ATGAA 5 cut(s) 470, 515, 528, 614, 633
TspGWI ACGGA 1 cut(s) 374
Tth111I GACNNNGTC 1 cut(s) 266
XapI RAATTY 1 cut(s) 455
XceI RCATGY 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 3 cut(s) 105, 320, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.