MD14G1097000.v1.1

Vacuolar protein-sorting-associated protein 37 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008384
Physical Location & Seq
Reverse (-)
14725467 .. 14728185
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1097000.v1.1.491

Sequence Viewer

Length: 708 bp
ATGTCCATCTTCAAGTTCTGGGGATCTCAGGAGGAACCTACTCAGCCGCGTCCGGAGGTTGTTACTCCTTCACAGTCATGGTACCCTCCATCTGTAGTTAGTTCTCCGACTTCTTCTCGCCCTGGGACACCGAGTAGCACGTCGTCTAGCAGTATAAGTTACCAAAGGCCAACAGAAAGGGCACAGTCTGCGTTACATGTTTCTCCCGCCGAAGCTGCCGGTGTTATTTCCGTTCTGAAGGACAAGAGTGTTGAGGACCTACGGAAGCTTTTGTCTGACAAGAATGCATACCAGCAATTTTTTATTTCACTCGATCAGGTCAAAATTCAAAATAATCTAAGAGAGGAGCTACGGAGGGAAACTCTGCAGTTATCCAGGGAAAACTTGGAAAAAGAACCGCAGATGGTGGAACTTAGAAACCAGTGCAGAATAATTAGAACAACAGAGTTGGCTGCTGCTCAGGAGAGGCTAAATGAACTCGAGAGAAAGAAAGAAGAAACGTTGAAGCTATGTTCCCCGTCTTCTCTTCTTCAAAGGCTTCAAGAATCACTGAATACGACAGAGGAGGAATCTGAAGCTCTGCACCAGCAGTTCCTTGACAAGGAGGGTGATCTTGGGACTTTTGTGGAAAAGTACAAGAAGCTTCGTACCACTTACCACAAGCGAGCGCTTGTTCATCTTGCCGCCAAAACATCTTCAATAGGGTGA

Protein Analysis

236

Amino Acids

26.75

Weight (kDa)

7.76

Isoelectric Point (pI)

66.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mod_r PF07200 80 - 224 5.1e-38 Modifier of rudimentary (Mod(r)) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 81
AccII CGCG 1 cut(s) 49
AccIII TCCGGA 1 cut(s) 52
AciI CCGC 4 cut(s) 47, 207, 398, 684
AclI AACGTT 1 cut(s) 500
AclWI GGATC 1 cut(s) 31
AcsI RAATTY 1 cut(s) 324
AcuI CTGAAG 2 cut(s) 257, 594
AfaI GTAC 3 cut(s) 83, 635, 649
AfeI AGCGCT 1 cut(s) 669
AfiI CCNNNNNNNGG 1 cut(s) 601
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 6 cut(s) 13, 329, 505, 533, 542, 699
AjiI CACGTC 1 cut(s) 141
AjnI CCWGG 2 cut(s) 121, 374
AluBI AGCT 6 cut(s) 215, 268, 349, 508, 578, 643
AluI AGCT 6 cut(s) 215, 268, 349, 508, 578, 643
AlwI GGATC 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 479
Aor13HI TCCGGA 1 cut(s) 52
Aor51HI AGCGCT 1 cut(s) 669
AoxI GGCC 1 cut(s) 167
ApeKI GCWGC 3 cut(s) 215, 452, 455
ApoI RAATTY 1 cut(s) 324
Asp718I GGTACC 1 cut(s) 81
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 620
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 202, 439, 442
BccI CCATC 3 cut(s) 14, 97, 397
BciT130I CCWGG 2 cut(s) 123, 376
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 2 cut(s) 93, 365
BfoI RGCGCY 1 cut(s) 671
BisI GCNGC 5 cut(s) 47, 216, 453, 456, 684
BlsI GCNGC 5 cut(s) 48, 217, 454, 457, 685
Bme1390I CCNGG 2 cut(s) 123, 376
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 479
BmgBI CACGTC 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 2 cut(s) 36, 83
BmrFI CCNGG 2 cut(s) 123, 376
BpiI GAAGAC 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 346, 378
Bpu10I CCTNAGC 1 cut(s) 459
BsaJI CCNNGG 3 cut(s) 121, 122, 375
BsaWI WCCGGW 1 cut(s) 52
Bsc4I CCNNNNNNNGG 1 cut(s) 601
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 421
BseAI TCCGGA 1 cut(s) 52
BseBI CCWGG 2 cut(s) 123, 376
BseDI CCNNGG 3 cut(s) 121, 122, 375
BseLI CCNNNNNNNGG 1 cut(s) 601
BseMII CTCAG 3 cut(s) 41, 56, 473
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 2 cut(s) 359, 578
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 3 cut(s) 202, 439, 442
BsgI GTGCAG 2 cut(s) 445, 566
Bsh1236I CGCG 1 cut(s) 49
BshFI GGCC 1 cut(s) 169
BshNI GGYRCC 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 2 cut(s) 53, 219
BslFI GGGAC 2 cut(s) 139, 631
BslI CCNNNNNNNGG 1 cut(s) 601
BsmFI GGGAC 2 cut(s) 139, 631
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 169
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 184
Bsp13I TCCGGA 1 cut(s) 52
Bsp143I GATC 3 cut(s) 23, 313, 610
BspACI CCGC 4 cut(s) 47, 207, 398, 684
BspANI GGCC 1 cut(s) 169
BspCNI CTCAG 3 cut(s) 40, 55, 472
BspEI TCCGGA 1 cut(s) 52
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 36, 83
BspMAI CTGCAG 1 cut(s) 369
BspPI GGATC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 1 cut(s) 421
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 3 cut(s) 121, 122, 375
BssMI GATC 3 cut(s) 23, 313, 610
Bst2UI CCWGG 2 cut(s) 123, 376
Bst4CI ACNGT 2 cut(s) 75, 186
Bst6I CTCTTC 1 cut(s) 531
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 666
BstDEI CTNAG 5 cut(s) 27, 42, 338, 413, 459
BstENI CCTNNNNNAGG 1 cut(s) 599
BstFNI CGCG 1 cut(s) 49
BstH2I RGCGCY 1 cut(s) 671
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 3 cut(s) 26, 316, 613
BstMBI GATC 3 cut(s) 23, 313, 610
BstMWI GCNNNNNNNGC 2 cut(s) 188, 215
BstNI CCWGG 2 cut(s) 123, 376
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 121, 374
BstSFI CTRYAG 2 cut(s) 93, 365
BstSLI GKGCMC 1 cut(s) 184
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 202, 439, 442
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
BsuRI GGCC 1 cut(s) 169
BtrI CACGTC 1 cut(s) 141
BtsIMutI CAGTG 2 cut(s) 428, 548
Cac8I GCNNGC 1 cut(s) 666
CfoI GCGC 1 cut(s) 670
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 256
CseI GACGC 1 cut(s) 38
Csp6I GTAC 3 cut(s) 82, 634, 648
CviAII CATG 2 cut(s) 78, 197
CviQI GTAC 3 cut(s) 82, 634, 648
DdeI CTNAG 5 cut(s) 27, 42, 338, 413, 459
DpnI GATC 3 cut(s) 25, 315, 612
DpnII GATC 3 cut(s) 23, 313, 610
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco47I GGWCC 1 cut(s) 256
Eco47III AGCGCT 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 257, 594
Eco88I CYCGRG 1 cut(s) 479
EcoNI CCTNNNNNAGG 1 cut(s) 599
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 121, 374
EcoT22I ATGCAT 1 cut(s) 289
FaeI CATG 2 cut(s) 81, 200
FaiI YATR 5 cut(s) 79, 155, 198, 289, 511
FaqI GGGAC 2 cut(s) 139, 631
FatI CATG 2 cut(s) 77, 196
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 5 cut(s) 47, 216, 453, 456, 684
Fsp4HI GCNGC 5 cut(s) 47, 216, 453, 456, 684
FspBI CTAG 1 cut(s) 147
GlaI GCGC 1 cut(s) 669
GluI GCNGC 5 cut(s) 47, 216, 453, 456, 684
HaeII RGCGCY 1 cut(s) 671
HaeIII GGCC 1 cut(s) 169
HapII CCGG 2 cut(s) 53, 219
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 670
Hin1II CATG 2 cut(s) 81, 200
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HindIII AAGCTT 2 cut(s) 266, 641
HinfI GANTC 2 cut(s) 545, 569
HpaII CCGG 2 cut(s) 53, 219
HphI GGTGA 1 cut(s) 620
Hpy188I TCNGA 4 cut(s) 108, 237, 277, 574
Hpy188III TCNNGA 5 cut(s) 29, 53, 461, 481, 542
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 2 cut(s) 78, 232
HpyCH4III ACNGT 2 cut(s) 75, 186
HpyCH4IV ACGT 2 cut(s) 140, 500
HpyCH4V TGCA 4 cut(s) 287, 367, 426, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 215
HpyF3I CTNAG 5 cut(s) 27, 42, 338, 413, 459
HpySE526I ACGT 2 cut(s) 140, 500
Hsp92II CATG 2 cut(s) 81, 200
HspAI GCGC 1 cut(s) 668
Kpn2I TCCGGA 1 cut(s) 52
KpnI GGTACC 1 cut(s) 85
Kzo9I GATC 3 cut(s) 23, 313, 610
LmnI GCTCC 1 cut(s) 346
Lsp1109I GCAGC 3 cut(s) 202, 439, 442
MaeI CTAG 1 cut(s) 147
MaeII ACGT 2 cut(s) 140, 500
MaeIII GTNAC 3 cut(s) 61, 158, 192
MalI GATC 3 cut(s) 25, 315, 612
MboI GATC 3 cut(s) 23, 313, 610
MboII GAAGA 6 cut(s) 105, 506, 513, 518, 521, 687
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 3 cut(s) 296, 324, 432
MmeI TCCRAC 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 289
MroI TCCGGA 1 cut(s) 52
MslI CAYNNNNRTG 1 cut(s) 76
MspI CCGG 2 cut(s) 53, 219
MspR9I CCNGG 2 cut(s) 123, 376
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 2 cut(s) 123, 376
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 2 cut(s) 188, 215
NdeII GATC 3 cut(s) 23, 313, 610
NlaIII CATG 2 cut(s) 81, 200
NlaIV GGNNCC 2 cut(s) 36, 83
NsiI ATGCAT 1 cut(s) 289
NspI RCATGY 1 cut(s) 200
PaeR7I CTCGAG 1 cut(s) 479
PasI CCCWGGG 1 cut(s) 122
PciI ACATGT 1 cut(s) 196
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 545, 569
PkrI GCNGC 5 cut(s) 48, 217, 454, 457, 685
PpuMI RGGWCCY 1 cut(s) 256
PscI ACATGT 1 cut(s) 196
Psp1406I AACGTT 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 121, 374
PspGI CCWGG 2 cut(s) 121, 374
PspN4I GGNNCC 2 cut(s) 36, 83
PspPI GGNCC 1 cut(s) 256
PspPPI RGGWCCY 1 cut(s) 256
PstI CTGCAG 1 cut(s) 369
PsuI RGATCY 1 cut(s) 23
RsaI GTAC 3 cut(s) 83, 635, 649
RsaNI GTAC 3 cut(s) 82, 634, 648
RseI CAYNNNNRTG 1 cut(s) 76
SatI GCNGC 5 cut(s) 47, 216, 453, 456, 684
Sau3AI GATC 3 cut(s) 23, 313, 610
Sau96I GGNCC 1 cut(s) 256
ScrFI CCNGG 2 cut(s) 123, 376
SduI GDGCHC 1 cut(s) 184
SfcI CTRYAG 2 cut(s) 93, 365
Sfr274I CTCGAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 256
SlaI CTCGAG 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 76
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 3 cut(s) 296, 324, 432
SsiI CCGC 4 cut(s) 47, 207, 398, 684
SspMI CTAG 1 cut(s) 147
StyD4I CCNGG 2 cut(s) 121, 374
TaaI ACNGT 2 cut(s) 75, 186
TaiI ACGT 2 cut(s) 143, 503
TaqI TCGA 2 cut(s) 312, 480
TasI AATT 3 cut(s) 296, 324, 432
TatI WGTACW 1 cut(s) 633
TauI GCSGC 2 cut(s) 49, 686
TfiI GAWTC 2 cut(s) 545, 569
TscAI CASTG 2 cut(s) 428, 555
TseI GCWGC 3 cut(s) 215, 452, 455
TspDTI ATGAA 2 cut(s) 489, 665
TspGWI ACGGA 3 cut(s) 220, 277, 367
TspRI CASTG 2 cut(s) 428, 555
VpaK11BI GGWCC 1 cut(s) 256
XagI CCTNNNNNAGG 1 cut(s) 599
XapI RAATTY 1 cut(s) 324
XceI RCATGY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XhoI CTCGAG 1 cut(s) 479
XspI CTAG 1 cut(s) 147
Zsp2I ATGCAT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.