Rroxscaffold_6G00410100

Vacuolar protein-sorting-associated protein 37 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008384
Physical Location & Seq
Reverse (-)
32537853 .. 32543427
5575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410100.1

Sequence Viewer

Length: 873 bp
ATGAGAATAAGAGCCAGTGTGTTAGAAAAAGGGGAGTTTAGAGTCCAGGCGCAAGAGTTCTTAGGGTTTACGAATAGTGATCCTCTCTCTCGCCCGACAGAGTTTCCAGCCTTTGCATTAATTGCAGCTAGCTTTAGAGCAGCCGAGCTTTGTGCTCTAAGGGCTGTGATGGTGGAAACCAACTCAGAAGAGGGAGTGAGGGGATCACAGGAGCCACAAGCTCAGCCACATCCTGAGGATGCTTCAGCACAGCAGTCCTATTATCCTCACTCTGTTAACTCCACGAGCTCTTCTCGTCCTTTAACTCCGAGTAGAACCTCTTCTGCTTCCAATTTGAGTTCTAGGGGTAACTCCCCCTCACCTGTTTCTCCCACCGAAGCTGCCGGTGTTATTGCTTCTTTGAAGGACAAAAGTGTTGATGAACTACGAAAGCTTTTGTCTGACAAGGATGCATACCATCAATTTTTCCTGTCGCTCGATCAGGTCAAGGTTCAAAATAATTTAAGGGAAGAGCTACGAAAGGAAACTCTGCAGTTAACTAGGGAAAACTTGGAAAAGGAACCGCGCATGGTGGAACTTAGAAACCAGTGCAGAATAATTAGAACGACAGAGTTGGCTGCCGCCCAAGAGAGGCTAAATGATCTTGAGCGACAGAAAGAAGAAACTTTGAAGTTATGTTCACCTTCTTCCCTTCTCCAAAGGCTTCAAGAAGCAATGAATAAGACGGAGGAGGAATCTGAAGACCTGCACAGGCAACTCCTCAATAGTGAAATCGATCTTGGGACTTTTGTTCCGAAATATAAGAAGCTCCGCAACACTTACCACCGGCGAGCGCTTGTTCATCTTGCAGCAAAAACATCTTCAATTGGCTGA

Protein Analysis

290

Amino Acids

32.65

Weight (kDa)

7.04

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mod_r PF07200 135 - 279 1.8e-38 Modifier of rudimentary (Mod(r)) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 753
AccII CGCG 1 cut(s) 565
AciI CCGC 3 cut(s) 563, 621, 811
AclWI GGATC 2 cut(s) 74, 211
AcuI CTGAAG 2 cut(s) 228, 759
AfeI AGCGCT 1 cut(s) 834
AfiI CCNNNNNNNGG 1 cut(s) 630
AgsI TTSAA 5 cut(s) 403, 494, 670, 707, 864
AjnI CCWGG 1 cut(s) 45
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AluBI AGCT 9 cut(s) 128, 132, 148, 221, 288, 380, 433, 514, 808
AluI AGCT 9 cut(s) 128, 132, 148, 221, 288, 380, 433, 514, 808
Alw21I GWGCWC 2 cut(s) 157, 290
AlwI GGATC 2 cut(s) 74, 211
Aor51HI AGCGCT 1 cut(s) 834
ApeKI GCWGC 5 cut(s) 125, 140, 380, 617, 848
AseI ATTAAT 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 319
AspLEI GCGC 3 cut(s) 52, 567, 835
AsuHPI GGTGA 2 cut(s) 351, 672
AsuNHI GCTAGC 1 cut(s) 128
AxyI CCTNAGG 1 cut(s) 234
BanII GRGCYC 1 cut(s) 290
BarI GAAGNNNNNNTAC 2 cut(s) 304, 336
BauI CACGAG 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 747
Bbv12I GWGCWC 2 cut(s) 157, 290
BbvI GCAGC 5 cut(s) 137, 152, 367, 604, 860
BccI CCATC 2 cut(s) 163, 465
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 1 cut(s) 47
BfaI CTAG 3 cut(s) 129, 342, 540
BfmI CTRYAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 836
BfuAI ACCTGC 1 cut(s) 753
BisI GCNGC 6 cut(s) 126, 141, 381, 618, 621, 849
BlpI GCTNAGC 1 cut(s) 222
BlsI GCNGC 6 cut(s) 127, 142, 382, 619, 622, 850
Bme1390I CCNGG 1 cut(s) 47
BmiI GGNNCC 2 cut(s) 213, 561
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 2 cut(s) 229, 439
BmtI GCTAGC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 747
BplI GAGNNNNNCTC 2 cut(s) 277, 309
Bpu1102I GCTNAGC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 665
Bsa29I ATCGAT 1 cut(s) 774
Bsc4I CCNNNNNNNGG 1 cut(s) 630
Bse118I RCCGGY 2 cut(s) 383, 825
Bse1I ACTGG 2 cut(s) 15, 586
Bse21I CCTNAGG 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 1 cut(s) 47
BseCI ATCGAT 1 cut(s) 774
BseGI GGATG 3 cut(s) 229, 244, 454
BseLI CCNNNNNNNGG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 3 cut(s) 198, 225, 236
BseNI ACTGG 2 cut(s) 15, 586
BseRI GAGGAG 2 cut(s) 743, 749
BseXI GCAGC 5 cut(s) 137, 152, 367, 604, 860
BsgI GTGCAG 2 cut(s) 610, 731
Bsh1236I CGCG 1 cut(s) 565
BshVI ATCGAT 1 cut(s) 774
BsiHKAI GWGCWC 2 cut(s) 157, 290
BsiSI CCGG 2 cut(s) 384, 826
BslFI GGGAC 1 cut(s) 796
BslI CCNNNNNNNGG 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 796
Bsp1286I GDGCHC 2 cut(s) 157, 290
Bsp143I GATC 5 cut(s) 79, 203, 478, 640, 775
Bsp1720I GCTNAGC 1 cut(s) 222
BspACI CCGC 3 cut(s) 563, 621, 811
BspCNI CTCAG 3 cut(s) 197, 226, 235
BspDI ATCGAT 1 cut(s) 774
BspFNI CGCG 1 cut(s) 565
BspLI GGNNCC 2 cut(s) 213, 561
BspMAI CTGCAG 1 cut(s) 534
BspMI ACCTGC 1 cut(s) 753
BspOI GCTAGC 1 cut(s) 132
BspPI GGATC 2 cut(s) 74, 211
BspQI GCTCTTC 2 cut(s) 295, 504
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 2 cut(s) 383, 825
BsrI ACTGG 2 cut(s) 15, 586
BssAI RCCGGY 2 cut(s) 383, 825
BssMI GATC 5 cut(s) 79, 203, 478, 640, 775
BssSI CACGAG 1 cut(s) 283
Bst2BI CACGAG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 47
Bst6I CTCTTC 4 cut(s) 183, 295, 325, 504
BstAPI GCANNNNNTGC 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 130, 831
BstDEI CTNAG 6 cut(s) 61, 158, 184, 222, 234, 578
BstF5I GGATG 3 cut(s) 229, 244, 454
BstFNI CGCG 1 cut(s) 565
BstH2I RGCGCY 1 cut(s) 836
BstHHI GCGC 3 cut(s) 52, 567, 835
BstKTI GATC 5 cut(s) 82, 206, 481, 643, 778
BstMBI GATC 5 cut(s) 79, 203, 478, 640, 775
BstMWI GCNNNNNNNGC 2 cut(s) 122, 161
BstNI CCWGG 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 530
BstUI CGCG 1 cut(s) 565
BstV1I GCAGC 5 cut(s) 137, 152, 367, 604, 860
BstV2I GAAGAC 1 cut(s) 747
Bsu15I ATCGAT 1 cut(s) 774
Bsu36I CCTNAGG 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 774
BtsCI GGATG 3 cut(s) 229, 244, 454
BtsIMutI CAGTG 2 cut(s) 22, 593
BveI ACCTGC 1 cut(s) 753
Cac8I GCNNGC 2 cut(s) 130, 831
CfoI GCGC 3 cut(s) 52, 567, 835
Cfr10I RCCGGY 2 cut(s) 383, 825
ClaI ATCGAT 1 cut(s) 774
CviAII CATG 1 cut(s) 568
DdeI CTNAG 6 cut(s) 61, 158, 184, 222, 234, 578
DpnI GATC 5 cut(s) 81, 205, 480, 642, 777
DpnII GATC 5 cut(s) 79, 203, 478, 640, 775
Eam1104I CTCTTC 4 cut(s) 183, 295, 325, 504
EarI CTCTTC 4 cut(s) 183, 295, 325, 504
Ecl136II GAGCTC 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 290
Eco47III AGCGCT 1 cut(s) 834
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 2 cut(s) 228, 759
Eco81I CCTNAGG 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 290
FaeI CATG 1 cut(s) 571
FaiI YATR 4 cut(s) 454, 569, 676, 801
FaqI GGGAC 1 cut(s) 796
FatI CATG 1 cut(s) 567
Fnu4HI GCNGC 6 cut(s) 126, 141, 381, 618, 621, 849
FokI GGATG 3 cut(s) 216, 251, 461
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 6 cut(s) 126, 141, 381, 618, 621, 849
FspBI CTAG 3 cut(s) 129, 342, 540
GlaI GCGC 3 cut(s) 51, 566, 834
GluI GCNGC 6 cut(s) 126, 141, 381, 618, 621, 849
HaeII RGCGCY 1 cut(s) 836
HapII CCGG 2 cut(s) 384, 826
HhaI GCGC 3 cut(s) 52, 567, 835
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 3 cut(s) 50, 565, 833
HinP1I GCGC 3 cut(s) 50, 565, 833
HincII GTYRAC 2 cut(s) 277, 537
HindII GTYRAC 2 cut(s) 277, 537
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 2 cut(s) 42, 734
HpaI GTTAAC 2 cut(s) 277, 537
HpaII CCGG 2 cut(s) 384, 826
HphI GGTGA 2 cut(s) 351, 672
Hpy166II GTNNAC 4 cut(s) 69, 277, 537, 680
Hpy188I TCNGA 5 cut(s) 187, 309, 442, 739, 795
Hpy188III TCNNGA 3 cut(s) 233, 644, 707
Hpy8I GTNNAC 4 cut(s) 69, 277, 537, 680
HpyAV CCTTC 3 cut(s) 397, 693, 701
HpyCH4V TGCA 7 cut(s) 116, 125, 452, 532, 591, 748, 848
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 161
HpyF3I CTNAG 6 cut(s) 61, 158, 184, 222, 234, 578
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 3 cut(s) 50, 565, 833
KspAI GTTAAC 2 cut(s) 277, 537
Kzo9I GATC 5 cut(s) 79, 203, 478, 640, 775
LguI GCTCTTC 2 cut(s) 295, 504
LmnI GCTCC 2 cut(s) 211, 813
Lsp1109I GCAGC 5 cut(s) 137, 152, 367, 604, 860
LweI GCATC 2 cut(s) 229, 439
MaeI CTAG 3 cut(s) 129, 342, 540
MaeIII GTNAC 1 cut(s) 347
MalI GATC 5 cut(s) 81, 205, 480, 642, 777
MboI GATC 5 cut(s) 79, 203, 478, 640, 775
MboII GAAGA 8 cut(s) 200, 282, 312, 521, 671, 678, 752, 852
MfeI CAATTG 1 cut(s) 864
MhlI GDGCHC 2 cut(s) 157, 290
MluCI AATT 6 cut(s) 120, 331, 461, 499, 597, 864
MlyI GAGTC 1 cut(s) 51
Mph1103I ATGCAT 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 319
MseI TTAA 5 cut(s) 119, 276, 302, 503, 536
MspI CCGG 2 cut(s) 384, 826
MspR9I CCNGG 1 cut(s) 47
MunI CAATTG 1 cut(s) 864
MvaI CCWGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 565
MwoI GCNNNNNNNGC 2 cut(s) 122, 161
NdeII GATC 5 cut(s) 79, 203, 478, 640, 775
NheI GCTAGC 1 cut(s) 128
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 2 cut(s) 213, 561
NmeAIII GCCGAG 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 454
PciSI GCTCTTC 2 cut(s) 295, 504
PdmI GAANNNNTTC 1 cut(s) 319
PfeI GAWTC 1 cut(s) 734
PkrI GCNGC 6 cut(s) 127, 142, 382, 619, 622, 850
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 213, 561
PstI CTGCAG 1 cut(s) 534
SacI GAGCTC 1 cut(s) 290
SapI GCTCTTC 2 cut(s) 295, 504
SaqAI TTAA 5 cut(s) 119, 276, 302, 503, 536
SatI GCNGC 6 cut(s) 126, 141, 381, 618, 621, 849
Sau3AI GATC 5 cut(s) 79, 203, 478, 640, 775
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 2 cut(s) 157, 290
SfaNI GCATC 2 cut(s) 229, 439
SfcI CTRYAG 1 cut(s) 530
SgrAI CRCCGGYG 1 cut(s) 825
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 6 cut(s) 120, 331, 461, 499, 597, 864
SsiI CCGC 3 cut(s) 563, 621, 811
SspMI CTAG 3 cut(s) 129, 342, 540
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 45
TaqI TCGA 2 cut(s) 477, 774
TasI AATT 6 cut(s) 120, 331, 461, 499, 597, 864
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 1 cut(s) 734
Tru1I TTAA 5 cut(s) 119, 276, 302, 503, 536
Tru9I TTAA 5 cut(s) 119, 276, 302, 503, 536
TscAI CASTG 2 cut(s) 22, 593
TseI GCWGC 5 cut(s) 125, 140, 380, 617, 848
TspDTI ATGAA 3 cut(s) 435, 731, 830
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 2 cut(s) 22, 593
VspI ATTAAT 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 319
XspI CTAG 3 cut(s) 129, 342, 540
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.