Rh3DG184000

Vacuolar protein-sorting-associated protein 37 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0008384
Physical Location & Seq
Reverse (-)
16287832 .. 16292528
4697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG184000.1

Sequence Viewer

Length: 687 bp
ATGTTCAAATTATGGGGATCACAGGAGCCACAAACTCAGCCACGTCCTGAGGATGCTTCAGCACAGCAGTCCTATTATCCTCACTCTGTTAACTCCCCGAGCTCTTCTCGTCCTTTAACTCCGAGTAGGACCTCTTCCGCTTCCAATTTGAGTTCTAGGGGTAACTCCCCCTCACCTGTTTCTCCCACCGAAGCTGCCGGTGTTATTGCCTCTTTGAAGGACAAAAGTGTTGATGAACTACGAAAGCTTTTGTCTGACAAGGATGCATACCATCAATTTTTCCTGTCGCTCGATCAGGCCAAGGTTCAAAATAATTTAAGGGAAGAGCTACGAAAGGAAACTCTGCAGTTAACTAGGGAAAACTTGGAAAAGGAACCGCGCATGGTGGAACTTAGAAACCAGTGCAGAATAATTAGAACGACAGAGTTGGCTACCGCCCAAGAGAGGCTAAATGAGCTTGAGCGACAGAAAGAAGAAACTTTGAAGTTATGTTCACCTTCTTCCCTTCTCCAAAGGCTTCAAGAAGCAATGAATAAGACGGAGGAGGAATCTGAAAACCTGCACAGGCAACTCCTCGATAGTGAAATCGATCTCGGGACTTTTGTTCCGAAATATAAGAAGCTCCGCAACACTTACCACCGGCGAGCGCTTGTTCATCTTGCAGCAAAAACATCTTCAATTGGCTGA

Protein Analysis

228

Amino Acids

26.0

Weight (kDa)

8.81

Isoelectric Point (pI)

67.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mod_r PF07200 73 - 217 3.6e-38 Modifier of rudimentary (Mod(r)) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 567
AccII CGCG 1 cut(s) 379
AciI CCGC 4 cut(s) 138, 377, 435, 625
AclWI GGATC 1 cut(s) 25
AcuI CTGAAG 1 cut(s) 42
AfeI AGCGCT 1 cut(s) 648
AfiI CCNNNNNNNGG 1 cut(s) 444
AgsI TTSAA 6 cut(s) 7, 217, 308, 484, 521, 678
AjiI CACGTC 1 cut(s) 44
AluBI AGCT 6 cut(s) 102, 194, 247, 328, 457, 622
AluI AGCT 6 cut(s) 102, 194, 247, 328, 457, 622
Alw21I GWGCWC 1 cut(s) 104
AlwI GGATC 1 cut(s) 25
Ama87I CYCGRG 2 cut(s) 97, 593
Aor51HI AGCGCT 1 cut(s) 648
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 194, 662
AspLEI GCGC 2 cut(s) 381, 649
AspS9I GGNCC 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 165, 486
AvaI CYCGRG 2 cut(s) 97, 593
AvaII GGWCC 1 cut(s) 129
AxyI CCTNAGG 1 cut(s) 48
BanII GRGCYC 1 cut(s) 104
BarI GAAGNNNNNNTAC 2 cut(s) 118, 150
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 2 cut(s) 181, 674
BccI CCATC 1 cut(s) 279
BfaI CTAG 2 cut(s) 156, 354
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 650
BfuAI ACCTGC 1 cut(s) 567
BisI GCNGC 2 cut(s) 195, 663
BlsI GCNGC 2 cut(s) 196, 664
Bme18I GGWCC 1 cut(s) 129
BmeT110I CYCGRG 2 cut(s) 97, 593
BmgBI CACGTC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 2 cut(s) 27, 375
BmsI GCATC 2 cut(s) 43, 253
BplI GAGNNNNNCTC 2 cut(s) 91, 123
BpuEI CTTGAG 1 cut(s) 479
Bsa29I ATCGAT 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 1 cut(s) 444
Bse118I RCCGGY 2 cut(s) 197, 639
Bse1I ACTGG 1 cut(s) 400
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 534
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 2 cut(s) 58, 268
BseLI CCNNNNNNNGG 1 cut(s) 444
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 39, 50
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 2 cut(s) 557, 563
BseXI GCAGC 2 cut(s) 181, 674
BsgI GTGCAG 2 cut(s) 424, 545
Bsh1236I CGCG 1 cut(s) 379
BshFI GGCC 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 588
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 2 cut(s) 97, 593
BsiSI CCGG 2 cut(s) 198, 640
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 610
BsnI GGCC 1 cut(s) 299
BsoBI CYCGRG 2 cut(s) 97, 593
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 3 cut(s) 17, 292, 589
BspACI CCGC 4 cut(s) 138, 377, 435, 625
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 2 cut(s) 40, 49
BspDI ATCGAT 1 cut(s) 588
BspFNI CGCG 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 27, 375
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 567
BspPI GGATC 1 cut(s) 25
BspQI GCTCTTC 2 cut(s) 109, 318
BsrDI GCAATG 1 cut(s) 534
BsrFI RCCGGY 2 cut(s) 197, 639
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 2 cut(s) 197, 639
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 3 cut(s) 17, 292, 589
BssT1I CCWWGG 1 cut(s) 300
Bst6I CTCTTC 3 cut(s) 109, 139, 318
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 3 cut(s) 36, 48, 392
BstF5I GGATG 2 cut(s) 58, 268
BstFNI CGCG 1 cut(s) 379
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 2 cut(s) 381, 649
BstKTI GATC 3 cut(s) 20, 295, 592
BstMBI GATC 3 cut(s) 17, 292, 589
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstSFI CTRYAG 1 cut(s) 344
BstUI CGCG 1 cut(s) 379
BstV1I GCAGC 2 cut(s) 181, 674
Bsu15I ATCGAT 1 cut(s) 588
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 1 cut(s) 299
BsuTUI ATCGAT 1 cut(s) 588
BtrI CACGTC 1 cut(s) 44
BtsCI GGATG 2 cut(s) 58, 268
BtsIMutI CAGTG 1 cut(s) 407
BveI ACCTGC 1 cut(s) 567
Cac8I GCNNGC 1 cut(s) 645
CfoI GCGC 2 cut(s) 381, 649
Cfr10I RCCGGY 2 cut(s) 197, 639
Cfr13I GGNCC 1 cut(s) 129
ClaI ATCGAT 1 cut(s) 588
CviAII CATG 1 cut(s) 382
DdeI CTNAG 3 cut(s) 36, 48, 392
DpnI GATC 3 cut(s) 19, 294, 591
DpnII GATC 3 cut(s) 17, 292, 589
Eam1104I CTCTTC 3 cut(s) 109, 139, 318
EarI CTCTTC 3 cut(s) 109, 139, 318
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 129
Eco47III AGCGCT 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 42
Eco81I CCTNAGG 1 cut(s) 48
Eco88I CYCGRG 2 cut(s) 97, 593
EcoICRI GAGCTC 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 300
EcoT22I ATGCAT 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 385
FaiI YATR 5 cut(s) 13, 268, 383, 490, 615
FaqI GGGAC 1 cut(s) 610
FatI CATG 1 cut(s) 381
Fnu4HI GCNGC 2 cut(s) 195, 663
FokI GGATG 2 cut(s) 65, 275
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 2 cut(s) 195, 663
FspBI CTAG 2 cut(s) 156, 354
GlaI GCGC 2 cut(s) 380, 648
GluI GCNGC 2 cut(s) 195, 663
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 1 cut(s) 299
HapII CCGG 2 cut(s) 198, 640
HhaI GCGC 2 cut(s) 381, 649
Hin1II CATG 1 cut(s) 385
Hin6I GCGC 2 cut(s) 379, 647
HinP1I GCGC 2 cut(s) 379, 647
HincII GTYRAC 2 cut(s) 91, 351
HindII GTYRAC 2 cut(s) 91, 351
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 1 cut(s) 548
HpaI GTTAAC 2 cut(s) 91, 351
HpaII CCGG 2 cut(s) 198, 640
HphI GGTGA 2 cut(s) 165, 486
Hpy166II GTNNAC 3 cut(s) 91, 351, 494
Hpy188I TCNGA 4 cut(s) 123, 256, 553, 609
Hpy188III TCNNGA 3 cut(s) 47, 521, 595
Hpy8I GTNNAC 3 cut(s) 91, 351, 494
HpyAV CCTTC 3 cut(s) 211, 507, 515
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 5 cut(s) 266, 346, 405, 562, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 3 cut(s) 36, 48, 392
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 1 cut(s) 385
HspAI GCGC 2 cut(s) 379, 647
KspAI GTTAAC 2 cut(s) 91, 351
Kzo9I GATC 3 cut(s) 17, 292, 589
LguI GCTCTTC 2 cut(s) 109, 318
LmnI GCTCC 2 cut(s) 25, 627
Lsp1109I GCAGC 2 cut(s) 181, 674
LweI GCATC 2 cut(s) 43, 253
MaeI CTAG 2 cut(s) 156, 354
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 1 cut(s) 161
MalI GATC 3 cut(s) 19, 294, 591
MboI GATC 3 cut(s) 17, 292, 589
MboII GAAGA 6 cut(s) 96, 126, 335, 485, 492, 666
MfeI CAATTG 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 6 cut(s) 8, 145, 275, 313, 411, 678
MnlI CCTC 9 cut(s) 43, 90, 142, 181, 220, 438, 535, 538, 584
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 4 cut(s) 90, 116, 317, 350
MspI CCGG 2 cut(s) 198, 640
MunI CAATTG 1 cut(s) 678
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 454
NdeII GATC 3 cut(s) 17, 292, 589
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 27, 375
NsiI ATGCAT 1 cut(s) 268
PciSI GCTCTTC 2 cut(s) 109, 318
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 2 cut(s) 196, 664
PpuMI RGGWCCY 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 104
Psp5II RGGWCCY 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 27, 375
PspPI GGNCC 1 cut(s) 129
PspPPI RGGWCCY 1 cut(s) 129
PstI CTGCAG 1 cut(s) 348
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 2 cut(s) 109, 318
SaqAI TTAA 4 cut(s) 90, 116, 317, 350
SatI GCNGC 2 cut(s) 195, 663
Sau3AI GATC 3 cut(s) 17, 292, 589
Sau96I GGNCC 1 cut(s) 129
SduI GDGCHC 1 cut(s) 104
SfaNI GCATC 2 cut(s) 43, 253
SfcI CTRYAG 1 cut(s) 344
SgrAI CRCCGGYG 1 cut(s) 639
SinI GGWCC 1 cut(s) 129
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 6 cut(s) 8, 145, 275, 313, 411, 678
SsiI CCGC 4 cut(s) 138, 377, 435, 625
SspMI CTAG 2 cut(s) 156, 354
SstI GAGCTC 1 cut(s) 104
StyI CCWWGG 1 cut(s) 300
TaiI ACGT 1 cut(s) 46
TaqI TCGA 3 cut(s) 291, 576, 588
TasI AATT 6 cut(s) 8, 145, 275, 313, 411, 678
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 4 cut(s) 90, 116, 317, 350
Tru9I TTAA 4 cut(s) 90, 116, 317, 350
TscAI CASTG 1 cut(s) 407
TseI GCWGC 2 cut(s) 194, 662
TspDTI ATGAA 3 cut(s) 249, 545, 644
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 129
XspI CTAG 2 cut(s) 156, 354
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.