AT2G36690

Feruloyl CoA ortho-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003921
Physical Location & Seq
Forward (+)
15379660 .. 15382131
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G36690.2

Sequence Viewer

Length: 1101 bp
ATGTCGCCGTCAATGATAGCTCCGGTGATGGAAGAAGAGAACAAGAAATACCAAAAAGGCGTGAAACATCTTTGTGAAAATGGTCTGACCAAAGTACCAACCAAGTATATATGGCCTGAACCTGACCGACCCATCTTGACAAAATCGGACAAGCTCATCAAACCAAACAAAAACCTAAAGCTCCCCCTCATAGATTTCGCTGAGCTCCTTGGACCTAACCGGCCTCATGTTCTACGAACCATAGCCGAGGCCTGCAAAACCTACGGCTTCTTTCAGGTTGTGAATCATGGGATGGAAGGAGACGTGAGCAAGAATATGATAGACGTGTGTAAGAGATTCTTTGAGCTTCCGTACGAGGAGAGATCCAAGTACATGTCGTCGGACATGTCGGCTCCAGTACGATACGGCACGAGTTTCAACCAGATCAAAGACAATGTCTTTTGTTGGAGAGACTTCTTGAAACTCTATGCACATCCACTCCCTGACTATCTTCCTCATTGGCCTTCTTCTCCTTCTGACTTCAGGAGTTCGGCGGCTACATACGCTAAAGAGACGAAAGAGATGTTCGAAATGATGGTCAAAGCCATCTTAGAAAGTCTTGAAATTGATGGCAGTGATGAGGCGGCTAAGGAGTTGGAAGAGGGAAGTCAGGTGGTGGTGGTGAACTGTTATCCACCGTGTCCGGAGCCAGAGCTGACGCTAGGGATGCCACCGCACTCAGACTACGGCTTCTTGACGCTTCTACTTCAAGACGAAGTCGAAGGTCTTCAAATTCTGTATCGTGACGAGTGGGTCACCGTTGATCCCATCCCTGGCTCTTTTGTTGTCAACGTAGGCGATCATCTCGAGATATTTAGCAATGGGAGGTATAAAAGCGTGCTACATAGGGTATTGGTGAACTCTACAAAACCTAGGATATCGGTGGCGTCGCTACACAGCTTCCCTTTGACTTCTGTCGTAAAGCCATCGCCGAAACTCGTCGATAAACATAATCCATCACAGTATATGGACACTGATTTTACCACTTTTCTTCAGTATATAACTTCTCGTGAACCCAAATGGAAGAATTTTCTTGAATCACGCAAGATTCACCATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

366

Amino Acids

41.95

Weight (kDa)

6.2

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 61 - 168 3.2e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 217 - 311 1.1e-35 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 791
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 3 cut(s) 533, 623, 713
AclWI GGATC 2 cut(s) 357, 797
AcsI RAATTY 2 cut(s) 771, 1066
AcuI CTGAAG 2 cut(s) 505, 1016
AcyI GRCGYC 1 cut(s) 926
AfaI GTAC 4 cut(s) 96, 353, 371, 399
AfiI CCNNNNNNNGG 1 cut(s) 812
AflIII ACRYGT 3 cut(s) 324, 372, 384
AgsI TTSAA 6 cut(s) 418, 460, 602, 749, 770, 1076
AjiI CACGTC 2 cut(s) 304, 325
AjnI CCWGG 1 cut(s) 811
AluBI AGCT 7 cut(s) 20, 154, 181, 205, 346, 694, 939
AluI AGCT 7 cut(s) 20, 154, 181, 205, 346, 694, 939
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 3 cut(s) 294, 444, 545
AlwI GGATC 2 cut(s) 357, 797
Ama87I CYCGRG 1 cut(s) 845
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 4 cut(s) 113, 221, 249, 500
ApoI RAATTY 2 cut(s) 771, 1066
ArsI GACNNNNNNTTYG 4 cut(s) 420, 452, 561, 593
Asp700I GAANNNNTTC 1 cut(s) 765
AspA2I CCTAGG 1 cut(s) 911
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 5 cut(s) 37, 673, 787, 907, 1082
AsuII TTCGAA 1 cut(s) 567
AvaI CYCGRG 1 cut(s) 845
AvaII GGWCC 1 cut(s) 212
AvrII CCTAGG 1 cut(s) 911
BaeI ACNNNNGTAYC 2 cut(s) 78, 111
BanII GRGCYC 1 cut(s) 207
BauI CACGAG 2 cut(s) 409, 1047
BbsI GAAGAC 1 cut(s) 758
Bbv12I GWGCWC 1 cut(s) 207
BccI CCATC 9 cut(s) 22, 140, 286, 568, 593, 602, 815, 973, 1003
BceAI ACGGC 3 cut(s) 280, 421, 742
BciT130I CCWGG 1 cut(s) 813
BcoDI GTCTC 3 cut(s) 294, 444, 545
BfaI CTAG 2 cut(s) 701, 912
BisI GCNGC 2 cut(s) 534, 624
BlnI CCTAGG 1 cut(s) 911
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 2 cut(s) 535, 625
Bme1390I CCNGG 1 cut(s) 813
Bme18I GGWCC 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 845
BmgBI CACGTC 2 cut(s) 304, 325
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 2 cut(s) 393, 687
BmrFI CCNGG 1 cut(s) 813
BmsI GCATC 1 cut(s) 696
BoxI GACNNNNGTC 1 cut(s) 953
BpiI GAAGAC 1 cut(s) 758
BpmI CTGGAG 1 cut(s) 378
Bpu10I CCTNAGC 1 cut(s) 627
Bpu1102I GCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 567
BsaHI GRCGYC 1 cut(s) 926
BsaJI CCNNGG 4 cut(s) 208, 246, 811, 911
BsaWI WCCGGW 2 cut(s) 22, 682
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 812
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 1 cut(s) 395
Bse3DI GCAATG 1 cut(s) 865
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 1 cut(s) 813
BseDI CCNNGG 4 cut(s) 208, 246, 811, 911
BseGI GGATG 4 cut(s) 297, 472, 711, 807
BseLI CCNNNNNNNGG 1 cut(s) 812
BseMI GCAATG 1 cut(s) 865
BseMII CTCAG 2 cut(s) 192, 732
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 371
BshFI GGCC 4 cut(s) 115, 223, 251, 502
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 3 cut(s) 23, 220, 683
BsiWI CGTACG 1 cut(s) 351
BslI CCNNNNNNNGG 1 cut(s) 812
BsmAI GTCTC 3 cut(s) 294, 444, 545
BsmBI CGTCTC 2 cut(s) 294, 545
BsnI GGCC 4 cut(s) 115, 223, 251, 502
BsoBI CYCGRG 1 cut(s) 845
Bsp119I TTCGAA 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 207
Bsp13I TCCGGA 1 cut(s) 682
Bsp143I GATC 4 cut(s) 362, 423, 802, 838
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 3 cut(s) 533, 623, 713
BspANI GGCC 4 cut(s) 115, 223, 251, 502
BspCNI CTCAG 2 cut(s) 193, 731
BspEI TCCGGA 1 cut(s) 682
BspLI GGNNCC 2 cut(s) 393, 687
BspPI GGATC 2 cut(s) 357, 797
BspT104I TTCGAA 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 865
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 4 cut(s) 208, 246, 811, 911
BssMI GATC 4 cut(s) 362, 423, 802, 838
BssNI GRCGYC 1 cut(s) 926
BssSI CACGAG 2 cut(s) 409, 1047
BssT1I CCWWGG 2 cut(s) 208, 911
Bst2BI CACGAG 2 cut(s) 409, 1047
Bst2UI CCWGG 1 cut(s) 813
Bst4CI ACNGT 4 cut(s) 668, 678, 799, 1002
Bst6I CTCTTC 2 cut(s) 30, 633
BstACI GRCGYC 1 cut(s) 926
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 253, 878
BstDEI CTNAG 4 cut(s) 201, 589, 627, 718
BstEII GGTNACC 1 cut(s) 793
BstF5I GGATG 4 cut(s) 297, 472, 711, 807
BstKTI GATC 4 cut(s) 365, 426, 805, 841
BstMAI GTCTC 3 cut(s) 294, 444, 545
BstMBI GATC 4 cut(s) 362, 423, 802, 838
BstMWI GCNNNNNNNGC 2 cut(s) 542, 706
BstNI CCWGG 1 cut(s) 813
BstNSI RCATGY 2 cut(s) 376, 388
BstPAI GACNNNNGTC 1 cut(s) 953
BstPI GGTNACC 1 cut(s) 793
BstSCI CCNGG 1 cut(s) 811
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 1 cut(s) 362
BstYI RGATCY 1 cut(s) 362
BsuRI GGCC 4 cut(s) 115, 223, 251, 502
BtgZI GCGATG 1 cut(s) 951
BtrI CACGTC 2 cut(s) 304, 325
BtsCI GGATG 4 cut(s) 297, 472, 711, 807
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 2 cut(s) 619, 1011
Cac8I GCNNGC 2 cut(s) 253, 878
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 3 cut(s) 706, 745, 915
Csp6I GTAC 4 cut(s) 95, 352, 370, 398
CviAII CATG 5 cut(s) 227, 287, 373, 385, 1094
CviQI GTAC 4 cut(s) 95, 352, 370, 398
DdeI CTNAG 4 cut(s) 201, 589, 627, 718
DpnI GATC 4 cut(s) 364, 425, 804, 840
DpnII GATC 4 cut(s) 362, 423, 802, 838
DrdI GACNNNNNNGTC 1 cut(s) 791
DseDI GACNNNNNNGTC 1 cut(s) 791
Eam1104I CTCTTC 2 cut(s) 30, 633
EarI CTCTTC 2 cut(s) 30, 633
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 2 cut(s) 208, 911
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 207
Eco32I GATATC 1 cut(s) 918
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 505, 1016
Eco88I CYCGRG 1 cut(s) 845
Eco91I GGTNACC 1 cut(s) 793
EcoICRI GAGCTC 1 cut(s) 205
EcoO65I GGTNACC 1 cut(s) 793
EcoRII CCWGG 1 cut(s) 811
EcoRV GATATC 1 cut(s) 918
EcoT14I CCWWGG 2 cut(s) 208, 911
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 2 cut(s) 208, 911
Esp3I CGTCTC 2 cut(s) 294, 545
FaeI CATG 5 cut(s) 230, 290, 376, 388, 1097
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 5 cut(s) 226, 286, 372, 384, 1093
Fnu4HI GCNGC 2 cut(s) 534, 624
FokI GGATG 4 cut(s) 304, 459, 718, 794
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 2 cut(s) 534, 624
FspBI CTAG 2 cut(s) 701, 912
GluI GCNGC 2 cut(s) 534, 624
GsuI CTGGAG 1 cut(s) 378
HaeIII GGCC 4 cut(s) 115, 223, 251, 502
HapII CCGG 3 cut(s) 23, 220, 683
HgaI GACGC 3 cut(s) 706, 745, 915
Hin1I GRCGYC 1 cut(s) 926
Hin1II CATG 5 cut(s) 230, 290, 376, 388, 1097
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HinfI GANTC 4 cut(s) 283, 336, 1076, 1087
HpaII CCGG 3 cut(s) 23, 220, 683
HphI GGTGA 5 cut(s) 37, 673, 787, 907, 1082
Hpy166II GTNNAC 4 cut(s) 664, 829, 898, 1052
Hpy188I TCNGA 5 cut(s) 87, 148, 382, 517, 721
Hpy8I GTNNAC 4 cut(s) 664, 829, 898, 1052
Hpy99I CGWCG 3 cut(s) 382, 931, 983
HpyAV CCTTC 4 cut(s) 290, 513, 522, 755
HpyCH4III ACNGT 4 cut(s) 668, 678, 799, 1002
HpyCH4IV ACGT 3 cut(s) 303, 324, 831
HpyCH4V TGCA 2 cut(s) 255, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 706
HpyF3I CTNAG 4 cut(s) 201, 589, 627, 718
HpySE526I ACGT 3 cut(s) 303, 324, 831
Hsp92I GRCGYC 1 cut(s) 926
Hsp92II CATG 5 cut(s) 230, 290, 376, 388, 1097
Kpn2I TCCGGA 1 cut(s) 682
Kzo9I GATC 4 cut(s) 362, 423, 802, 838
LmnI GCTCC 5 cut(s) 25, 186, 210, 397, 685
LweI GCATC 1 cut(s) 696
MaeI CTAG 2 cut(s) 701, 912
MaeII ACGT 3 cut(s) 303, 324, 831
MaeIII GTNAC 2 cut(s) 782, 793
MalI GATC 4 cut(s) 364, 425, 804, 840
MboI GATC 4 cut(s) 362, 423, 802, 838
MboII GAAGA 8 cut(s) 44, 47, 482, 498, 650, 758, 1022, 1075
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 3 cut(s) 603, 771, 1066
MmeI TCCRAC 3 cut(s) 360, 425, 615
MnlI CCTC 8 cut(s) 197, 234, 241, 349, 504, 613, 634, 858
MroI TCCGGA 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 72
MspI CCGG 3 cut(s) 23, 220, 683
MspR9I CCNGG 1 cut(s) 813
MvaI CCWGG 1 cut(s) 813
MwoI GCNNNNNNNGC 2 cut(s) 542, 706
NdeII GATC 4 cut(s) 362, 423, 802, 838
NlaIII CATG 5 cut(s) 230, 290, 376, 388, 1097
NlaIV GGNNCC 2 cut(s) 393, 687
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 2 cut(s) 782, 793
NspI RCATGY 2 cut(s) 376, 388
NspV TTCGAA 1 cut(s) 567
PaeR7I CTCGAG 1 cut(s) 845
PceI AGGCCT 1 cut(s) 251
PciI ACATGT 2 cut(s) 372, 384
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 4 cut(s) 283, 336, 1076, 1087
Pfl23II CGTACG 1 cut(s) 351
PflFI GACNNNGTC 2 cut(s) 434, 755
PkrI GCNGC 2 cut(s) 535, 625
PscI ACATGT 2 cut(s) 372, 384
PshAI GACNNNNGTC 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 811
PspEI GGTNACC 1 cut(s) 793
PspGI CCWGG 1 cut(s) 811
PspLI CGTACG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 393, 687
PspPI GGNCC 1 cut(s) 212
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PsuI RGATCY 1 cut(s) 362
PsyI GACNNNGTC 2 cut(s) 434, 755
RsaI GTAC 4 cut(s) 96, 353, 371, 399
RsaNI GTAC 4 cut(s) 95, 352, 370, 398
RseI CAYNNNNRTG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 207
SatI GCNGC 2 cut(s) 534, 624
Sau3AI GATC 4 cut(s) 362, 423, 802, 838
Sau96I GGNCC 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 813
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 1 cut(s) 696
Sfr274I CTCGAG 1 cut(s) 845
SfuI TTCGAA 1 cut(s) 567
SinI GGWCC 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
Sse9I AATT 3 cut(s) 603, 771, 1066
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 3 cut(s) 533, 623, 713
SspMI CTAG 2 cut(s) 701, 912
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 811
StyI CCWWGG 2 cut(s) 208, 911
TaaI ACNGT 4 cut(s) 668, 678, 799, 1002
TaiI ACGT 3 cut(s) 306, 327, 834
TaqI TCGA 4 cut(s) 567, 759, 846, 981
TaqII GACCGA 1 cut(s) 141
TasI AATT 3 cut(s) 603, 771, 1066
TatI WGTACW 1 cut(s) 369
TauI GCSGC 2 cut(s) 536, 626
TfiI GAWTC 4 cut(s) 283, 336, 1076, 1087
TscAI CASTG 2 cut(s) 619, 1018
TseFI GTSAC 2 cut(s) 782, 793
Tsp45I GTSAC 2 cut(s) 782, 793
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 2 cut(s) 619, 1018
Tth111I GACNNNGTC 2 cut(s) 434, 755
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 2 cut(s) 771, 1066
XceI RCATGY 2 cut(s) 376, 388
XhoI CTCGAG 1 cut(s) 845
XmaJI CCTAGG 1 cut(s) 911
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 2 cut(s) 701, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.