Rorug01G0125400

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003921
Physical Location & Seq
Reverse (-)
21483427 .. 21484725
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0125400.1

Sequence Viewer

Length: 1299 bp
ATGAGTTCTGTTTCCAGATTATCAACTGTAGAAGTAATGAGAGTAGCAGGAGAAAGGTTCATCCAATTCTCTTCCCAAAAGTATGCCGATGACTCCATACCTAAACATGTTTTAGGCTGTGCTCCCCTAGGTCTCTCAGACCAGGAAGTCAAAGAGGTTGAGCTTGCACACCTTCTTTTAGCTTTTGCAGATAAGGTTGGAAAGCAACAATATGATAGGGCGAGAAAACTGCAGAATCTATGCCATTTCCTATCTTCCAGCACTGGAAATACTGTTCAGAGAGTTGTTTATTATTTTTCTAAAGCTCTTCGAGAAAGGACAGATAGGGAAACTGGGAGAAACACATCTAAGGGCTCAGAAAGTAAGGATTTACTTGCAATGCATTTAGAAAGGACAATGGCTTGTTGTCCTGCTTTCTTTGCTTGTTTCCTCGAACTTCCCTTCTATCAAGTAACTCAGTTTGCCGGGATCCAAGCAATTGTGGAAAGTGTAGCATTGGCCAAAAGAGTTCATTTCATTGACCTTGAGATCAGAAGTGGGGGGCACTGCATAGTGTTGATGCAAGCTCTTGCGTCAAGATATGAACAACCAATTGAGATGCTAAAAGTTACTGCAGTTGGAAGTGGAACTGCATCAAGACAAAAGCTAGAGGACACCGGCAAAAGGTTGGCTCAGTTTGCTGAGGCATTACATTTGCCCTTTTCCTTTAAAATAGCAACGGTTACAGACATCAACAACCTCAATGAAGATGCCTTCGAGTCTGAGGATGGTGAAGCAACTGCAGTCTACTGTTGTTTAATGCGAAGCAGCATGAGGACCCTACCTGATTGCTTGGAATCTGCAATAACAGAGCTCAGAAAGCTCAATCCATGTCTAATGGTGATCACAGAAATTGAAGCAAATCTCTATTCACCAGTTTTTGAAGACCGTTTCAATGAAGCGGTCTTCTACTATAGTGCTTATTTTGATTGCCTTGATGTCTGTATGGATAATTCCAGTCCATACAGGAGGGAATTGGAAGAGACTTTTTTGGCGCAACAGATTACAAATATTATAGCAAGTGAAGAAGAGAGGATATTCAGGCACATGAAGATTGATGTTTGGAGGAGTTTGTTTGCAAAGTTTGGAATGGTGGAAGCGGAGCTTAGCTTGTCATCGTTGTACCAAGCAGACCTTACTGCTAAGCAATTTGCATGTGGGAAATCATGCACATTGGATATGAATGGGAAATGCCTCACCATTGGATGGAAAGGAACAACAATTCTCTCTCTTTCTGCTTGGAAGTTCCATGCAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

432

Amino Acids

48.62

Weight (kDa)

5.82

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 56 - 428 5.7e-75 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 1245
AccI GTMKAC 1 cut(s) 786
AciI CCGC 2 cut(s) 941, 1139
AclWI GGATC 2 cut(s) 463, 476
AcoI YGGCCR 1 cut(s) 498
AfaI GTAC 1 cut(s) 1163
AfiI CCNNNNNNNGG 2 cut(s) 663, 1245
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 3 cut(s) 896, 923, 934
AjnI CCWGG 1 cut(s) 141
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 9 cut(s) 163, 182, 305, 566, 646, 853, 862, 1144, 1149
AluI AGCT 9 cut(s) 163, 182, 305, 566, 646, 853, 862, 1144, 1149
Alw21I GWGCWC 2 cut(s) 124, 855
Alw26I GTCTC 2 cut(s) 137, 1016
AlwI GGATC 2 cut(s) 463, 476
AoxI GGCC 1 cut(s) 498
ApeKI GCWGC 2 cut(s) 807, 1292
AspA2I CCTAGG 1 cut(s) 127
AspLEI GCGC 1 cut(s) 1036
AspS9I GGNCC 1 cut(s) 816
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 4 cut(s) 782, 892, 903, 1228
AvaII GGWCC 1 cut(s) 816
AvrII CCTAGG 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 546
BalI TGGCCA 1 cut(s) 500
BamHI GGATCC 1 cut(s) 468
BanII GRGCYC 2 cut(s) 356, 855
BbsI GAAGAC 2 cut(s) 930, 937
Bbv12I GWGCWC 2 cut(s) 124, 855
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 1 cut(s) 819
BccI CCATC 2 cut(s) 761, 1239
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 882
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 2 cut(s) 137, 1016
BfaI CTAG 2 cut(s) 128, 647
BfmI CTRYAG 5 cut(s) 27, 230, 612, 780, 952
BisI GCNGC 2 cut(s) 808, 1293
BlnI CCTAGG 1 cut(s) 127
BlpI GCTNAGC 2 cut(s) 1145, 1182
BlsI GCNGC 2 cut(s) 809, 1294
Bme1390I CCNGG 2 cut(s) 143, 466
Bme18I GGWCC 1 cut(s) 816
BmgT120I GGNCC 1 cut(s) 816
BmiI GGNNCC 2 cut(s) 470, 818
BmrFI CCNGG 2 cut(s) 143, 466
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 4 cut(s) 549, 588, 641, 739
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 2 cut(s) 930, 937
Bpu10I CCTNAGC 1 cut(s) 681
Bpu1102I GCTNAGC 2 cut(s) 1145, 1182
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 466
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 127
Bsc4I CCNNNNNNNGG 2 cut(s) 663, 1245
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 4 cut(s) 268, 337, 914, 996
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 3 cut(s) 60, 772, 1250
BseLI CCNNNNNNNGG 2 cut(s) 663, 1245
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 7 cut(s) 150, 369, 470, 672, 686, 753, 868
BseNI ACTGG 4 cut(s) 268, 337, 914, 996
BseRI GAGGAG 1 cut(s) 1120
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 1 cut(s) 819
BshFI GGCC 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 124, 855
BsiSI CCGG 2 cut(s) 465, 657
BslI CCNNNNNNNGG 2 cut(s) 663, 1245
BsmAI GTCTC 2 cut(s) 137, 1016
BsnI GGCC 1 cut(s) 500
Bso31I GGTCTC 1 cut(s) 137
Bsp1286I GDGCHC 4 cut(s) 124, 356, 546, 855
Bsp143I GATC 3 cut(s) 468, 528, 882
Bsp1720I GCTNAGC 2 cut(s) 1145, 1182
BspACI CCGC 2 cut(s) 941, 1139
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 7 cut(s) 149, 368, 469, 673, 685, 754, 867
BspLI GGNNCC 2 cut(s) 470, 818
BspMAI CTGCAG 3 cut(s) 234, 616, 784
BspPI GGATC 2 cut(s) 463, 476
BspQI GCTCTTC 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 656
BsrI ACTGG 4 cut(s) 268, 337, 914, 996
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 3 cut(s) 468, 528, 882
BssT1I CCWWGG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 5 cut(s) 28, 274, 721, 791, 929
Bst6I CTCTTC 4 cut(s) 76, 312, 1014, 1062
BstC8I GCNNGC 2 cut(s) 165, 564
BstF5I GGATG 3 cut(s) 60, 772, 1250
BstHHI GCGC 1 cut(s) 1036
BstKTI GATC 3 cut(s) 471, 531, 885
BstMAI GTCTC 2 cut(s) 137, 1016
BstMBI GATC 3 cut(s) 468, 528, 882
BstMWI GCNNNNNNNGC 3 cut(s) 419, 677, 859
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 2 cut(s) 110, 1197
BstSCI CCNGG 2 cut(s) 141, 464
BstSFI CTRYAG 5 cut(s) 27, 230, 612, 780, 952
BstSLI GKGCMC 1 cut(s) 546
BstV1I GCAGC 1 cut(s) 819
BstV2I GAAGAC 2 cut(s) 930, 937
BstX2I RGATCY 1 cut(s) 468
BstYI RGATCY 1 cut(s) 468
BsuRI GGCC 1 cut(s) 500
BtsCI GGATG 3 cut(s) 60, 772, 1250
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 261, 544
Cac8I GCNNGC 2 cut(s) 165, 564
CfoI GCGC 1 cut(s) 1036
Cfr10I RCCGGY 1 cut(s) 656
Cfr13I GGNCC 1 cut(s) 816
CseI GACGC 1 cut(s) 561
Csp6I GTAC 1 cut(s) 1162
CviAII CATG 7 cut(s) 107, 811, 870, 1087, 1194, 1206, 1289
CviQI GTAC 1 cut(s) 1162
DpnI GATC 3 cut(s) 470, 530, 884
DpnII GATC 3 cut(s) 468, 528, 882
DraI TTTAAA 1 cut(s) 709
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 498
Eam1104I CTCTTC 4 cut(s) 76, 312, 1014, 1062
EarI CTCTTC 4 cut(s) 76, 312, 1014, 1062
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 356, 855
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 816
Eco53kI GAGCTC 1 cut(s) 853
EcoICRI GAGCTC 1 cut(s) 853
EcoO109I RGGNCCY 1 cut(s) 816
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 127
EcoT22I ATGCAT 1 cut(s) 384
EcoT38I GRGCYC 2 cut(s) 356, 855
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 7 cut(s) 110, 814, 873, 1090, 1197, 1209, 1292
FalI AAGNNNNNCTT 4 cut(s) 1128, 1160, 1158, 1190
FatI CATG 7 cut(s) 106, 810, 869, 1086, 1193, 1205, 1288
FbaI TGATCA 1 cut(s) 882
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 2 cut(s) 808, 1293
FokI GGATG 3 cut(s) 47, 779, 1257
FriOI GRGCYC 2 cut(s) 356, 855
Fsp4HI GCNGC 2 cut(s) 808, 1293
FspBI CTAG 2 cut(s) 128, 647
GlaI GCGC 1 cut(s) 1035
GluI GCNGC 2 cut(s) 808, 1293
HaeIII GGCC 1 cut(s) 500
HapII CCGG 2 cut(s) 465, 657
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 1036
Hin1II CATG 7 cut(s) 110, 814, 873, 1090, 1197, 1209, 1292
Hin6I GCGC 1 cut(s) 1034
HinP1I GCGC 1 cut(s) 1034
HinfI GANTC 4 cut(s) 92, 235, 758, 836
HpaII CCGG 2 cut(s) 465, 657
HphI GGTGA 4 cut(s) 782, 892, 903, 1228
Hpy166II GTNNAC 1 cut(s) 787
Hpy188I TCNGA 6 cut(s) 139, 279, 358, 533, 763, 857
Hpy188III TCNNGA 4 cut(s) 15, 311, 576, 636
Hpy8I GTNNAC 1 cut(s) 787
HpyAV CCTTC 3 cut(s) 182, 451, 763
HpyCH4III ACNGT 5 cut(s) 28, 274, 721, 791, 929
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 677, 859
Hsp92II CATG 7 cut(s) 110, 814, 873, 1090, 1197, 1209, 1292
HspAI GCGC 1 cut(s) 1034
Ksp22I TGATCA 1 cut(s) 882
Kzo9I GATC 3 cut(s) 468, 528, 882
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 127, 1141
Lsp1109I GCAGC 1 cut(s) 819
LweI GCATC 4 cut(s) 549, 588, 641, 739
MaeI CTAG 2 cut(s) 128, 647
MaeIII GTNAC 3 cut(s) 451, 607, 721
MalI GATC 3 cut(s) 470, 530, 884
MboI GATC 3 cut(s) 468, 528, 882
MfeI CAATTG 2 cut(s) 477, 591
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 4 cut(s) 124, 356, 546, 855
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 8 cut(s) 65, 477, 591, 891, 991, 1013, 1187, 1260
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 2 cut(s) 86, 767
MmeI TCCRAC 2 cut(s) 178, 598
Mox20I TGGCCA 1 cut(s) 500
Mph1103I ATGCAT 1 cut(s) 384
MscI TGGCCA 1 cut(s) 500
MseI TTAA 2 cut(s) 708, 797
Msp20I TGGCCA 1 cut(s) 500
MspI CCGG 2 cut(s) 465, 657
MspR9I CCNGG 2 cut(s) 143, 466
MunI CAATTG 2 cut(s) 477, 591
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 419, 677, 859
NciI CCSGG 1 cut(s) 466
NdeII GATC 3 cut(s) 468, 528, 882
NlaIII CATG 7 cut(s) 110, 814, 873, 1090, 1197, 1209, 1292
NlaIV GGNNCC 2 cut(s) 470, 818
NsiI ATGCAT 1 cut(s) 384
NspI RCATGY 2 cut(s) 110, 1197
PciI ACATGT 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 235, 836
PflMI CCANNNNNTGG 1 cut(s) 1245
PkrI GCNGC 2 cut(s) 809, 1294
PleI GAGTC 2 cut(s) 86, 766
PpsI GAGTC 2 cut(s) 86, 766
PpuMI RGGWCCY 1 cut(s) 816
PscI ACATGT 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 470, 818
PspPI GGNCC 1 cut(s) 816
PspPPI RGGWCCY 1 cut(s) 816
PstI CTGCAG 3 cut(s) 234, 616, 784
PsuI RGATCY 1 cut(s) 468
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
SacI GAGCTC 1 cut(s) 855
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 2 cut(s) 708, 797
SatI GCNGC 2 cut(s) 808, 1293
Sau3AI GATC 3 cut(s) 468, 528, 882
Sau96I GGNCC 1 cut(s) 816
SchI GAGTC 2 cut(s) 86, 767
ScrFI CCNGG 2 cut(s) 143, 466
SduI GDGCHC 4 cut(s) 124, 356, 546, 855
SfaNI GCATC 4 cut(s) 549, 588, 641, 739
SfcI CTRYAG 5 cut(s) 27, 230, 612, 780, 952
SinI GGWCC 1 cut(s) 816
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 8 cut(s) 65, 477, 591, 891, 991, 1013, 1187, 1260
SsiI CCGC 2 cut(s) 941, 1139
SspI AATATT 1 cut(s) 1051
SspMI CTAG 2 cut(s) 128, 647
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 2 cut(s) 141, 464
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 5 cut(s) 28, 274, 721, 791, 929
TaqI TCGA 3 cut(s) 310, 432, 756
TasI AATT 8 cut(s) 65, 477, 591, 891, 991, 1013, 1187, 1260
TfiI GAWTC 2 cut(s) 235, 836
Tru1I TTAA 2 cut(s) 708, 797
Tru9I TTAA 2 cut(s) 708, 797
TscAI CASTG 2 cut(s) 268, 551
TseI GCWGC 2 cut(s) 807, 1292
TspDTI ATGAA 8 cut(s) 49, 500, 505, 597, 759, 951, 1103, 1235
TspRI CASTG 2 cut(s) 268, 551
Van91I CCANNNNNTGG 1 cut(s) 1245
VpaK11BI GGWCC 1 cut(s) 816
XceI RCATGY 2 cut(s) 110, 1197
XmaJI CCTAGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 786
XspI CTAG 2 cut(s) 128, 647
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.