Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003921

Physical Location & Seq

Genomic Coordinates Forward (+)

16259237 .. 16262196

Gene Structure 2960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1103300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1281 bp

ATGTTTAGTTCTTCATTTTTTTCCACTATATATCCCTCGGAAACCTTCTCATTATTTCCATCTTCATTTCTTGTTGTTTATTCCCACTATATTTCCTTGTTCATTATCATTCTCCTTTTTGGAAAAGGACAGAGGTTAGGTTTTGGAATATTTCTCATGTCTCCAACGATGGCAGTGTTGACAACTACCGAGAATGGGGTAAGTGATCAGTACCAAAATGGCGTGAAGTGCTTGTATGAGAATGGTATAAACAAACTTCCAAACAAGTACATACTGCCCACTTTAGAACGACCTAACCGGAGTGATGACCAGGAACCAAAAGTATCCAACCAAAATCTGAAGCTGCCCATCATCGATTTCAAGGAACTGCAAGGTCCCAATAGACCCCAAGTCATTAAATCTCTGGCTAATGCTTGTGAACAATATGGCTTCTTTCAGTTGGTAAACCATGGCATTCCAAGTGATATTATAAGCAGTATGACCGACGTGAGCACTAGGTTTTTTGAGCTCCCTTTTGAGGAGAGAGCCAAGTACATGTCCTCAGATATGCGTGCGCCTGTCCGATATGGGACTAGCTTTAACCAGAACAAAGATAAGGTGTTTTATTGGAGGGATTTCTTGAAGCTAATGTGCCATCCCTTACCAGATGTCCTTTCTCACTGGCCTTCGTCACCGGTGGACATGAGGAAACTGGCGGCTACCTACGCAGAGGAAAACAAATACTTATTCCTAATGCTAATGGATGCCATTTTTGAGAGCTTACGAATTGTGGGAACCACAGAGAACACGACAGAAGAAGATGAAATGTTAAAGGACTTCAAAGATGGGAGCCAGCTAATGGTTGTCAATTGCTACCCGCCATGCCCCCAACCTGATCTAACCCTAGGGATGCCTCCCCACTCGGATTATGGGTTTCTCACAATTCTTCTCCAAGATGAAGTTGAAGGCCTTCAAATTAAAAAACAAGATAAATGGATCACCGTCGAACCAATCCCCAATTCTTTTGTGGTCAACGTTGGTGATCACCTTGAGATATTTAGCAATGGAAAATACAAGAGTGTTCTACATAGAGTGTTGGTGAATTCTTTAAAAGGTCGGATTTCTGTGGCCTCTTTGCATACTCTACCTTTCACGCGCTTGGTTGGACCGTCACCAAAACTTATCAACGAATCAAATCCAAGGCGGTACAAGGACACTAATTTTGCCAGCTTTCTGGAGTATATTTCATCTCGTGAGCCTAAATGGAAGAATTTCCTCGAGTCTAGGAAGTTATTGACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

427

Amino Acids

48.86

Weight (kDa)

6.72

Isoelectric Point (pI)

42.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	115 - 222	2.2e-29	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	278 - 372	4.8e-36	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36690 AT2G36690
fragaria_vesca	FvH4_6g17160 FvH4_7g06780 FvH4_7g06780
malus_domestica	MD07G1074700.v1.1 MD12G1103300.v1.1
prunus_persica	Prupe.2G095000_v2.0.a1 Prupe.7G123400_v2.0.a1 Prupe.7G123400_v2.0.a1
pyrus_communis	pycom07g05870 pycom12g10180
rosa_chinensis	RchiOBHm_Chr1g0336381 RchiOBHm_Chr3g0471251
rosa_laevigata	RLG00000024194 RLG00000029370
rosa_multiflora	Rmu_sc0000393.1_g000004 Rmu_sc0000393.1_g000005 Rmu_sc0004870.1_g000002 Rmu_sc0005261.1_g000001
rosa_roxburghii	Rroxscaffold_4G00315710 Rroxscaffold_6G00410170
rosa_rugosa	Rorug01G0125400 Rorug03G0116300
rosa_samantha	Rh1AG144600 Rh1BG114200 Rh1CG136100 Rh1DG151300 Rh3AG165700 Rh3BG191100 Rh3CG181400 Rh3DG184600
rosa_wichuraiana	Rw1G011860 Rw3G015600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	470
AccB7I	CCANNNNNTGG	1 cut(s)	838
AccII	CGCG	1 cut(s)	1135
AciI	CCGC	3 cut(s)	695, 857, 1183
AclI	AACGTT	1 cut(s)	1014
AclWI	GGATC	1 cut(s)	983
AcsI	RAATTY	2 cut(s)	1081, 1249
AcuI	CTGAAG	1 cut(s)	359
AfaI	GTAC	4 cut(s)	212, 269, 533, 1187
AfiI	CCNNNNNNNGG	3 cut(s)	195, 517, 838
AflIII	ACRYGT	1 cut(s)	534
AgeI	ACCGGT	1 cut(s)	673
AgsI	TTSAA	5 cut(s)	361, 622, 820, 944, 953
AhdI	GACNNNNNGTC	1 cut(s)	389
AjiI	CACGTC	1 cut(s)	487
AjnI	CCWGG	1 cut(s)	309
AjuI	GAANNNNNNNTTGG	2 cut(s)	982, 1014
AluBI	AGCT	7 cut(s)	343, 508, 576, 625, 759, 835, 1209
AluI	AGCT	7 cut(s)	343, 508, 576, 625, 759, 835, 1209
Alw21I	GWGCWC	2 cut(s)	494, 510
Alw26I	GTCTC	1 cut(s)	165
AlwI	GGATC	1 cut(s)	983
Ama87I	CYCGRG	1 cut(s)	1256
AoxI	GGCC	3 cut(s)	662, 946, 1107
ApeKI	GCWGC	1 cut(s)	343
ApoI	RAATTY	2 cut(s)	1081, 1249
ArsI	GACNNNNNNTTYG	2 cut(s)	1184, 1216
AsiGI	ACCGGT	1 cut(s)	673
Asp700I	GAANNNNTTC	2 cut(s)	948, 1250
AspA2I	CCTAGG	1 cut(s)	883
AspLEI	GCGC	2 cut(s)	556, 1137
AspS9I	GGNCC	2 cut(s)	374, 1145
AsuHPI	GGTGA	6 cut(s)	663, 970, 1016, 1031, 1090, 1143
AvaI	CYCGRG	1 cut(s)	1256
AvaII	GGWCC	2 cut(s)	374, 1145
AvrII	CCTAGG	1 cut(s)	883
BanII	GRGCYC	1 cut(s)	510
BauI	CACGAG	1 cut(s)	1230
Bbv12I	GWGCWC	2 cut(s)	494, 510
BbvI	GCAGC	1 cut(s)	330
BccI	CCATC	5 cut(s)	67, 163, 356, 642, 818
BciT130I	CCWGG	1 cut(s)	311
BciVI	GTATCC	1 cut(s)	334
BclI	TGATCA	2 cut(s)	205, 1021
BcoDI	GTCTC	1 cut(s)	165
BfaI	CTAG	5 cut(s)	495, 573, 884, 1263, 1279
BfuI	GTATCC	1 cut(s)	334
BisI	GCNGC	2 cut(s)	344, 696
BlnI	CCTAGG	1 cut(s)	883
BlsI	GCNGC	2 cut(s)	345, 697
Bme1390I	CCNGG	1 cut(s)	311
Bme18I	GGWCC	2 cut(s)	374, 1145
BmeRI	GACNNNNNGTC	1 cut(s)	389
BmeT110I	CYCGRG	1 cut(s)	1256
BmgBI	CACGTC	1 cut(s)	487
BmgT120I	GGNCC	2 cut(s)	374, 1145
BmiI	GGNNCC	4 cut(s)	315, 376, 775, 830
BmrFI	CCNGG	1 cut(s)	311
BmsI	GCATC	2 cut(s)	733, 879
BpmI	CTGGAG	1 cut(s)	1235
BpuEI	CTTGAG	1 cut(s)	1049
Bsa29I	ATCGAT	1 cut(s)	354
BsaJI	CCNNGG	4 cut(s)	36, 448, 883, 1178
BsaWI	WCCGGW	2 cut(s)	297, 673
Bsc4I	CCNNNNNNNGG	3 cut(s)	195, 517, 838
Bse118I	RCCGGY	1 cut(s)	673
Bse1I	ACTGG	2 cut(s)	665, 696
Bse3DI	GCAATG	1 cut(s)	1048
BseBI	CCWGG	1 cut(s)	311
BseCI	ATCGAT	1 cut(s)	354
BseDI	CCNNGG	4 cut(s)	36, 448, 883, 1178
BseGI	GGATG	3 cut(s)	634, 748, 894
BseLI	CCNNNNNNNGG	3 cut(s)	195, 517, 838
BseMI	GCAATG	1 cut(s)	1048
BseMII	CTCAG	1 cut(s)	555
BseNI	ACTGG	2 cut(s)	665, 696
BseRI	GAGGAG	1 cut(s)	533
BseXI	GCAGC	1 cut(s)	330
Bsh1236I	CGCG	1 cut(s)	1135
BshFI	GGCC	3 cut(s)	664, 948, 1109
BshTI	ACCGGT	1 cut(s)	673
BshVI	ATCGAT	1 cut(s)	354
BsiHKAI	GWGCWC	2 cut(s)	494, 510
BsiHKCI	CYCGRG	1 cut(s)	1256
BsiSI	CCGG	2 cut(s)	298, 674
BslFI	GGGAC	2 cut(s)	360, 583
BslI	CCNNNNNNNGG	3 cut(s)	195, 517, 838
BsmAI	GTCTC	1 cut(s)	165
BsmFI	GGGAC	2 cut(s)	360, 583
BsmI	GAATGC	1 cut(s)	453
BsnI	GGCC	3 cut(s)	664, 948, 1109
BsoBI	CYCGRG	1 cut(s)	1256
Bsp1286I	GDGCHC	2 cut(s)	494, 510
Bsp143I	GATC	4 cut(s)	205, 874, 975, 1021
Bsp19I	CCATGG	1 cut(s)	448
BspACI	CCGC	3 cut(s)	695, 857, 1183
BspANI	GGCC	3 cut(s)	664, 948, 1109
BspCNI	CTCAG	1 cut(s)	554
BspDI	ATCGAT	1 cut(s)	354
BspFNI	CGCG	1 cut(s)	1135
BspLI	GGNNCC	4 cut(s)	315, 376, 775, 830
BspPI	GGATC	1 cut(s)	983
BsrDI	GCAATG	1 cut(s)	1048
BsrFI	RCCGGY	1 cut(s)	673
BsrI	ACTGG	2 cut(s)	665, 696
BssAI	RCCGGY	1 cut(s)	673
BssECI	CCNNGG	4 cut(s)	36, 448, 883, 1178
BssMI	GATC	4 cut(s)	205, 874, 975, 1021
BssSI	CACGAG	1 cut(s)	1230
BssT1I	CCWWGG	3 cut(s)	448, 883, 1178
Bst2BI	CACGAG	1 cut(s)	1230
Bst2UI	CCWGG	1 cut(s)	311
Bst4CI	ACNGT	2 cut(s)	982, 1149
BstC8I	GCNNGC	3 cut(s)	552, 833, 1207
BstDEI	CTNAG	1 cut(s)	541
BstDSI	CCRYGG	1 cut(s)	448
BstF5I	GGATG	3 cut(s)	634, 748, 894
BstFNI	CGCG	1 cut(s)	1135
BstHHI	GCGC	2 cut(s)	556, 1137
BstKTI	GATC	4 cut(s)	208, 877, 978, 1024
BstMAI	GTCTC	1 cut(s)	165
BstMBI	GATC	4 cut(s)	205, 874, 975, 1021
BstMWI	GCNNNNNNNGC	2 cut(s)	228, 704
BstNI	CCWGG	1 cut(s)	311
BstNSI	RCATGY	1 cut(s)	538
BstSCI	CCNGG	1 cut(s)	309
BstUI	CGCG	1 cut(s)	1135
BstV1I	GCAGC	1 cut(s)	330
BstXI	CCANNNNNNTGG	1 cut(s)	1213
Bsu15I	ATCGAT	1 cut(s)	354
BsuI	GTATCC	1 cut(s)	334
BsuRI	GGCC	3 cut(s)	664, 948, 1109
BsuTUI	ATCGAT	1 cut(s)	354
BtgI	CCRYGG	1 cut(s)	448
BtrI	CACGTC	1 cut(s)	487
BtsCI	GGATG	3 cut(s)	634, 748, 894
BtsI	GCAGTG	1 cut(s)	180
BtsIMutI	CAGTG	2 cut(s)	180, 658
Cac8I	GCNNGC	3 cut(s)	552, 833, 1207
CfoI	GCGC	2 cut(s)	556, 1137
Cfr10I	RCCGGY	1 cut(s)	673
Cfr13I	GGNCC	2 cut(s)	374, 1145
ClaI	ATCGAT	1 cut(s)	354
Csp6I	GTAC	4 cut(s)	211, 268, 532, 1186
CspAI	ACCGGT	1 cut(s)	673
CviAII	CATG	5 cut(s)	157, 449, 535, 682, 861
CviQI	GTAC	4 cut(s)	211, 268, 532, 1186
DdeI	CTNAG	1 cut(s)	541
DpnI	GATC	4 cut(s)	207, 876, 977, 1023
DpnII	GATC	4 cut(s)	205, 874, 975, 1021
DraI	TTTAAA	1 cut(s)	1089
DriI	GACNNNNNGTC	1 cut(s)	389
Eam1105I	GACNNNNNGTC	1 cut(s)	389
Ecl136II	GAGCTC	1 cut(s)	508
Eco130I	CCWWGG	3 cut(s)	448, 883, 1178
Eco147I	AGGCCT	1 cut(s)	948
Eco24I	GRGCYC	1 cut(s)	510
Eco47I	GGWCC	2 cut(s)	374, 1145
Eco53kI	GAGCTC	1 cut(s)	508
Eco57I	CTGAAG	1 cut(s)	359
Eco88I	CYCGRG	1 cut(s)	1256
EcoICRI	GAGCTC	1 cut(s)	508
EcoO109I	RGGNCCY	1 cut(s)	374
EcoRI	GAATTC	1 cut(s)	1081
EcoRII	CCWGG	1 cut(s)	309
EcoT14I	CCWWGG	3 cut(s)	448, 883, 1178
EcoT38I	GRGCYC	1 cut(s)	510
ErhI	CCWWGG	3 cut(s)	448, 883, 1178
FaeI	CATG	5 cut(s)	160, 452, 538, 685, 864
FaqI	GGGAC	2 cut(s)	360, 583
FatI	CATG	5 cut(s)	156, 448, 534, 681, 860
FauI	CCCGC	1 cut(s)	864
FbaI	TGATCA	2 cut(s)	205, 1021
Fnu4HI	GCNGC	2 cut(s)	344, 696
FokI	GGATG	3 cut(s)	621, 755, 901
FriOI	GRGCYC	1 cut(s)	510
Fsp4HI	GCNGC	2 cut(s)	344, 696
FspBI	CTAG	5 cut(s)	495, 573, 884, 1263, 1279
GlaI	GCGC	2 cut(s)	555, 1136
GluI	GCNGC	2 cut(s)	344, 696
GsuI	CTGGAG	1 cut(s)	1235
HaeIII	GGCC	3 cut(s)	664, 948, 1109
HapII	CCGG	2 cut(s)	298, 674
HhaI	GCGC	2 cut(s)	556, 1137
Hin1II	CATG	5 cut(s)	160, 452, 538, 685, 864
Hin6I	GCGC	2 cut(s)	554, 1135
HinP1I	GCGC	2 cut(s)	554, 1135
HincII	GTYRAC	2 cut(s)	180, 1012
HindII	GTYRAC	2 cut(s)	180, 1012
HinfI	GANTC	2 cut(s)	1169, 1259
HpaII	CCGG	2 cut(s)	298, 674
HphI	GGTGA	6 cut(s)	663, 970, 1016, 1031, 1090, 1143
Hpy166II	GTNNAC	5 cut(s)	180, 419, 445, 679, 1012
Hpy188I	TCNGA	6 cut(s)	40, 339, 544, 563, 904, 1098
Hpy188III	TCNNGA	3 cut(s)	619, 1214, 1232
Hpy8I	GTNNAC	5 cut(s)	180, 419, 445, 679, 1012
Hpy99I	CGWCG	2 cut(s)	488, 986
HpyAV	CCTTC	4 cut(s)	55, 675, 938, 959
HpyCH4III	ACNGT	2 cut(s)	982, 1149
HpyCH4IV	ACGT	2 cut(s)	486, 1014
HpyCH4V	TGCA	2 cut(s)	370, 1117
HpyF10VI	GCNNNNNNNGC	2 cut(s)	228, 704
HpyF3I	CTNAG	1 cut(s)	541
HpySE526I	ACGT	2 cut(s)	486, 1014
Hsp92II	CATG	5 cut(s)	160, 452, 538, 685, 864
HspAI	GCGC	2 cut(s)	554, 1135
Ksp22I	TGATCA	2 cut(s)	205, 1021
Kzo9I	GATC	4 cut(s)	205, 874, 975, 1021
LmnI	GCTCC	2 cut(s)	513, 828
Lsp1109I	GCAGC	1 cut(s)	330
LweI	GCATC	2 cut(s)	733, 879
MaeI	CTAG	5 cut(s)	495, 573, 884, 1263, 1279
MaeII	ACGT	2 cut(s)	486, 1014
MaeIII	GTNAC	2 cut(s)	669, 1149
MalI	GATC	4 cut(s)	207, 876, 977, 1023
MboI	GATC	4 cut(s)	205, 874, 975, 1021
MboII	GAAGA	6 cut(s)	3, 54, 806, 809, 917, 1258
MfeI	CAATTG	1 cut(s)	847
MhlI	GDGCHC	2 cut(s)	494, 510
MluCI	AATT	8 cut(s)	765, 847, 921, 954, 997, 1081, 1198, 1249
MlyI	GAGTC	1 cut(s)	1268
MmeI	TCCRAC	4 cut(s)	188, 351, 1076, 1123
MroXI	GAANNNNTTC	2 cut(s)	948, 1250
MseI	TTAA	5 cut(s)	396, 579, 809, 957, 1088
MspI	CCGG	2 cut(s)	298, 674
MspR9I	CCNGG	1 cut(s)	311
MunI	CAATTG	1 cut(s)	847
Mva1269I	GAATGC	1 cut(s)	453
MvaI	CCWGG	1 cut(s)	311
MvnI	CGCG	1 cut(s)	1135
MwoI	GCNNNNNNNGC	2 cut(s)	228, 704
NcoI	CCATGG	1 cut(s)	448
NdeII	GATC	4 cut(s)	205, 874, 975, 1021
NlaIII	CATG	5 cut(s)	160, 452, 538, 685, 864
NlaIV	GGNNCC	4 cut(s)	315, 376, 775, 830
NmuCI	GTSAC	2 cut(s)	669, 1149
NspI	RCATGY	1 cut(s)	538
PaeR7I	CTCGAG	1 cut(s)	1256
PceI	AGGCCT	1 cut(s)	948
PciI	ACATGT	1 cut(s)	534
PctI	GAATGC	1 cut(s)	453
PdmI	GAANNNNTTC	2 cut(s)	948, 1250
PfeI	GAWTC	1 cut(s)	1169
PflMI	CCANNNNNTGG	1 cut(s)	838
PinAI	ACCGGT	1 cut(s)	673
PkrI	GCNGC	2 cut(s)	345, 697
PleI	GAGTC	1 cut(s)	1267
PpsI	GAGTC	1 cut(s)	1267
PpuMI	RGGWCCY	1 cut(s)	374
PscI	ACATGT	1 cut(s)	534
PsiI	TTATAA	1 cut(s)	470
Psp124BI	GAGCTC	1 cut(s)	510
Psp1406I	AACGTT	1 cut(s)	1014
Psp5II	RGGWCCY	1 cut(s)	374
Psp6I	CCWGG	1 cut(s)	309
PspGI	CCWGG	1 cut(s)	309
PspN4I	GGNNCC	4 cut(s)	315, 376, 775, 830
PspPI	GGNCC	2 cut(s)	374, 1145
PspPPI	RGGWCCY	1 cut(s)	374
PspXI	VCTCGAGB	1 cut(s)	1256
RsaI	GTAC	4 cut(s)	212, 269, 533, 1187
RsaNI	GTAC	4 cut(s)	211, 268, 532, 1186
SacI	GAGCTC	1 cut(s)	510
SaqAI	TTAA	5 cut(s)	396, 579, 809, 957, 1088
SatI	GCNGC	2 cut(s)	344, 696
Sau3AI	GATC	4 cut(s)	205, 874, 975, 1021
Sau96I	GGNCC	2 cut(s)	374, 1145
SchI	GAGTC	1 cut(s)	1268
ScrFI	CCNGG	1 cut(s)	311
SduI	GDGCHC	2 cut(s)	494, 510
SfaNI	GCATC	2 cut(s)	733, 879
Sfr274I	CTCGAG	1 cut(s)	1256
SgrAI	CRCCGGYG	1 cut(s)	673
SinI	GGWCC	2 cut(s)	374, 1145
SlaI	CTCGAG	1 cut(s)	1256
SmlI	CTYRAG	2 cut(s)	1028, 1256
SmoI	CTYRAG	2 cut(s)	1028, 1256
Sse9I	AATT	8 cut(s)	765, 847, 921, 954, 997, 1081, 1198, 1249
SseBI	AGGCCT	1 cut(s)	948
SsiI	CCGC	3 cut(s)	695, 857, 1183
SspI	AATATT	1 cut(s)	150
SspMI	CTAG	5 cut(s)	495, 573, 884, 1263, 1279
SstI	GAGCTC	1 cut(s)	510
StuI	AGGCCT	1 cut(s)	948
StyD4I	CCNGG	1 cut(s)	309
StyI	CCWWGG	3 cut(s)	448, 883, 1178
TaaI	ACNGT	2 cut(s)	982, 1149
TaiI	ACGT	2 cut(s)	489, 1017
TaqI	TCGA	3 cut(s)	354, 984, 1257
TaqII	GACCGA	1 cut(s)	497
TasI	AATT	8 cut(s)	765, 847, 921, 954, 997, 1081, 1198, 1249
TatI	WGTACW	2 cut(s)	267, 531
TauI	GCSGC	1 cut(s)	698
TfiI	GAWTC	1 cut(s)	1169
Tru1I	TTAA	5 cut(s)	396, 579, 809, 957, 1088
Tru9I	TTAA	5 cut(s)	396, 579, 809, 957, 1088
TscAI	CASTG	2 cut(s)	180, 665
TseFI	GTSAC	2 cut(s)	669, 1149
TseI	GCWGC	1 cut(s)	343
Tsp45I	GTSAC	2 cut(s)	669, 1149
TspDTI	ATGAA	5 cut(s)	54, 91, 816, 951, 1215
TspRI	CASTG	2 cut(s)	180, 665
Van91I	CCANNNNNTGG	1 cut(s)	838
VpaK11BI	GGWCC	2 cut(s)	374, 1145
XapI	RAATTY	2 cut(s)	1081, 1249
XceI	RCATGY	1 cut(s)	538
XcmI	CCANNNNNNNNNTGG	2 cut(s)	905, 1003
XhoI	CTCGAG	1 cut(s)	1256
XmaJI	CCTAGG	1 cut(s)	883
XmnI	GAANNNNTTC	2 cut(s)	948, 1250
XspI	CTAG	5 cut(s)	495, 573, 884, 1263, 1279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.