AT2G40030

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006658
Physical Location & Seq
Forward (+)
16714460 .. 16723718
9259 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40030.1

Sequence Viewer

Length: 5931 bp
ATGGAGGAAGAATCTACATCAGAGATTCTTGACGGAGAGATTGTTGGAATCACATTTGCTCTTGCTAGTCACCATGAAATATGTATTCAATCGATTAGTGAATCCGCAATTAATCATCCCAGTCAACTCACTAACGCATTCTTGGGGTTACCTCTTGAATTTGGTAAATGTGAATCTTGCGGTGCGACAGAACCAGATAAATGTGAAGGTCATTTTGGTTATATTCAGCTTCCAGTGCCAATATACCATCCTGCTCATGTTAATGAGTTGAAACAAATGCTGAGCCTTCTGTGCTTGAAATGTCTGAAGATAAAGAAGGCAAAGGGCACAAGTGGTGGTCTGGCGGATCGCTTGTTGGGTGTCTGTTGTGAGGAAGCTTCGCAAATCTCAATCAAAGATAGAGCATCAGATGGTGCCTCATATCTAGAACTGAAGCTCCCATCTAGGTCAAGACTGCAGCCTGGTTGCTGGAATTTCCTGGAAAGATATGGTTATCGCTATGGAAGCGATTACACGCGTCCCCTGCTGGCAAGAGAGGTTAAGGAAATTCTAAGACGGATCCCCGAAGAATCAAGAAAGAAACTCACGGCAAAGGGACATATTCCTCAAGAAGGATACATACTAGAGTATCTTCCTGTCCCGCCTAACTGTTTGTCAGTGCCAGAGGCTTCTGATGGTTTCAGCACCATGTCAGTGGATCCTTCCAGAATTGAGCTAAAGGACGTTCTGAAGAAAGTCATAGCCATAAAAAGCTCTAGGTCTGGCGAGACAAACTTTGAGTCGCATAAGGCTGAAGCCAGTGAGATGTTTAGAGTGGTGGATACCTATCTCCAGGTGAGGGGTACTGCAAAAGCGGCAAGAAACATTGATATGAGATACGGGGTGTCTAAGATCTCGGATAGTTCATCTTCAAAAGCTTGGACAGAAAAAATGAGAACACTTTTCATCCGTAAGGGTTCAGGTTTCTCTTCCCGTAGCGTCATCACTGGAGATGCTTACCGACATGTGAATGAGGTAGGGATCCCAATAGAAATTGCGCAGAGAATCACATTTGAGGAAAGGGTTAGTGTCCACAATAGAGGTTATCTACAAAAGTTGGTAGACGACAAGTTGTGTTTGAGTTATACTCAAGGTTCTACGACCTATTCTTTAAGGGATGGATCCAAGGGGCACACTGAGCTTAAACCCGGGCAGGTTGTTCATCGCAGGGTCATGGATGGGGATGTTGTGTTTATCAACAGACCCCCGACAACACATAAACATTCTCTTCAAGCGCTTCGGGTGTATGTTCATGAAGACAACACCGTGAAGATTAACCCTCTGATGTGTAGCCCCCTCAGTGCTGATTTTGATGGTGATTGTGTCCATTTGTTCTACCCTCAGTCTCTTAGTGCTAAGGCAGAGGTTATGGAGCTCTTCTCTGTGGAGAAGCAACTGCTTAGCTCACATACTGGACAGCTGATTTTGCAGATGGGTAGTGATTCTTTACTGTCTTTGAGGGTAATGCTGGAGCGGGTATTTCTGGACAAGGCAACTGCTCAACAGTTAGCTATGTATGGCTCTCTGTCCCTGCCACCACCAGCACTAAGGAAGTCCAGTAAATCTGGCCCAGCTTGGACAGTTTTCCAGATTCTACAGCTTGCCTTTCCAGAACGTCTGAGTTGCAAAGGAGACAGATTCCTGGTTGATGGAAGTGATTTGCTGAAGTTTGATTTCGGGGTTGACGCGATGGGGTCCATCATCAATGAAATTGTAACTTCAATCTTCCTGGAGAAGGGTCCAAAGGAGACACTTGGATTCTTTGATTCGCTGCAGCCTCTATTGATGGAAAGTCTGTTTGCTGAGGGATTTAGTCTAAGTTTGGAAGACTTATCCATGTCTAGGGCAGACATGGATGTAATTCACAACCTAATAATCCGAGAGATTTCACCTATGGTGTCTCGCTTGAGATTATCATATAGAGATGAATTGCAGCTAGAGAACAGCATCCACAAAGTGAAGGAAGTGGCTGCTAACTTTATGCTGAAGTCATATTCCATTCGGAACTTGATTGATATCAAGAGTAACTCAGCAATCACCAAGCTGGTGCAGCAGACAGGCTTCTTGGGTCTTCAGCTTTCTGACAAGAAGAAGTTTTACACGAAGACTCTGGTAGAGGACATGGCTATCTTTTGTAAGAGAAAGTATGGCAGGATTTCTTCATCTGGTGATTTTGGAATAGTGAAGGGCTGCTTCTTTCATGGGCTGGATCCGTATGAAGAGATGGCTCATTCAATTGCAGCTAGGGAGGTAATTGTTCGCTCTTCAAGGGGATTAGCTGAGCCTGGAACCCTCTTTAAGAACCTGATGGCTGTTCTTCGGGATATCGTGATTACAAACGATGGAACTGTAAGAAACACCTGTAGTAACTCAGTCATCCAGTTCAAATATGGAGTTGATTCTGAACGTGGACATCAAGGTCTGTTTGAAGCTGGAGAACCTGTTGGAGTATTGGCAGCTACTGCCATGTCAAATCCTGCATATAAGGCAGTGCTTGATTCCTCTCCAAATAGTAATTCATCATGGGAGCTAATGAAGGAAGTTCTGCTCTGCAAAGTCAATTTCCAAAACACTACTAATGATCGGCGCGTAATCCTTTATTTAAACGAATGCCATTGTGGAAAAAGGTTTTGCCAGGAAAATGCTGCTTGCACAGTCAGGAACAAGCTGAATAAAGTTAGTCTTAAAGATACTGCAGTGGAATTCCTAGTCGAATATAGAAAGCAACCAACTATATCAGAAATTTTTGGAATTGATTCATGCCTACATGGCCATATTCATCTTAATAAGACATTGCTGCAAGATTGGAACATCAGTATGCAGGATATACATCAAAAGTGTGAGGATGTAATTAACTCACTAGGACAAAAGAAAAAGAAGAAAGCTACTGATGATTTCAAGAGAACAAGTTTGTCTGTCAGTGAGTGCTGCTCTTTCCGAGATCCTTGTGGGAGCAAAGGTTCGGATATGCCTTGTTTGACGTTCTCCTACAATGCCACCGATCCCGATCTAGAACGAACTCTTGATGTTCTTTGCAATACAGTTTACCCAGTTCTGCTGGAAATAGTGATCAAAGGTGATTCACGGATTTGCTCAGCAAATATAATCTGGAATAGTTCAGACATGACAACGTGGATCCGGAATCGTCATGCATCCCGCCGAGGGGAATGGGTTTTGGATGTCACTGTTGAAAAATCTGCTGTCAAGCAAAGTGGTGATGCATGGAGGGTTGTGATAGACTCATGTCTTTCAGTGCTTCATCTAATAGACACAAAACGGTCAATCCCTTATTCTGTAAAACAAGTGCAAGAATTGCTTGGGTTATCCTGTGCTTTTGAGCAAGCAGTACAGCGTCTATCAGCTTCAGTGAGAATGGTCTCCAAGGGAGTTTTGAAAGAACACATCATTCTCCTAGCAAACAACATGACTTGTTCAGGGACTATGTTGGGTTTCAATTCTGGAGGTTACAAAGCTTTGACCCGATCCTTAAACATCAAGGCTCCATTCACAGAAGCAACTCTGATTGCACCAAGAAAATGTTTTGAGAAAGCGGCTGAGAAATGTCATACAGATTCACTATCAACAGTCGTTGGGTCTTGTTCTTGGGGAAAACGCGTGGATGTTGGTACAGGCTCACAGTTTGAGCTCCTATGGAACCAAAAAGAGACTGGTCTAGATGATAAAGAAGAAACAGATGTGTACAGCTTCCTTCAAATGGTCATATCCACGACAAACGCAGATGCATTTGTTTCTTCCCCTGGTTTTGATGTTACAGAGGAAGAAATGGCTGAATGGGCTGAATCGCCCGAGCGAGACTCTGCTCTTGGAGAGCCCAAGTTTGAGGACAGTGCCGACTTCCAAAATTTACATGACGAAGGCAAGCCATCAGGAGCCAACTGGGAGAAAAGCTCTTCGTGGGACAATGGTTGCAGTGGTGGTTCAGAGTGGGGAGTCTCAAAAAGTACAGGTGGTGAGGCAAACCCGGAGTCTAATTGGGAAAAGACTACAAATGTTGAGAAGGAAGATGCTTGGTCTAGTTGGAACACTAGAAAAGATGCTCAAGAATCTTCCAAGTCAGATTCAGGGGGTGCTTGGGGTATAAAGACAAAGGATGCTGATGCAGATACAACACCAAACTGGGAAACAAGTCCAGCTCCGAAGGATTCCATTGTCCCAGAAAATAATGAGCCGACTTCTGACGTTTGGGGACACAAATCTGTTTCAGACAAATCGTGGGACAAAAAGAACTGGGGAACTGAATCAGCTCCTGCTGCATGGGGTTCTACAGATGCTGCTGTTTGGGGTTCTAGTGATAAAAAGAACTCGGAAACAGAATCAGATGCTGCTGCTTGGGGTTCCAGGGACAAAAATAATTCTGACGTTGGGTCAGGTGCTGGTGTTTTAGGTCCCTGGAACAAGAAGAGCTCGGAAACTGAGTCAAATGGTGCTACTTGGGGTTCTAGTGACAAAACCAAATCAGGTGCTGCTGCATGGAATTCTTGGGACAAAAAGAACATAGAAACTGACTCAGAACCTGCTGCTTGGGGTTCTCAGGGAAAAAAGAATTCTGAAACTGAGTCAGGTCCTGCTGCTTGGGGTGCTTGGGACAAAAAGAAATCGGAAACTGAACCAGGTCCTGCTGGTTGGGGTATGGGGGACAAAAAGAATTCTGAAACTGAATTAGGCCCTGCTGCTATGGGTAATTGGGACAAAAAGAAATCGGATACGAAATCAGGTCCAGCTGCTTGGGGATCTACAGATGCTGCTGCTTGGGGTTCTAGTGACAAAAACAACTCGGAAACTGAATCAGATGCTGCTGCTTGGGGTTCTAGGAACAAAAAGACTTCTGAAATTGAATCAGGTGCTGGCGCTTGGGGTTCGTGGGGACAGCCAAGTCCCACTGCAGAAGACAAGGATACCAATGAGGATGATAGAAATCCGTGGGTTTCCTTGAAGGAGACCAAAAGTAGAGAGAAGGATGATAAAGAGAGAAGCCAGTGGGGAAATCCAGCCAAGAAATTTCCATCTAGTGGTGGCTGGTCCAATGGTGGTGGTGCAGACTGGAAAGGAAATAGGAACCATACTCCTAGGCCACCTAGATCTGAGGATAATTTGGCTCCCATGTTTACAGCAACGAGACAGCGCTTGGACTCATTCACCTCTGAGGAGCAAGAGCTGCTCTCAGATGTTGAGCCAGTCATGAGGACTCTCAGGAAAATTATGCATCCGTCTGCGTACCCTGATGGAGACCCAATTTCTGATGATGACAAGACGTTTGTCCTGGAGAAGATTTTGAACTTCCATCCCCAAAAAGAGACAAAGCTTGGTTCTGGCGTTGACTTCATCACGGTTGATAAGCACACCATCTTCTCAGACAGCAGGTGCTTCTTTGTTGTCTCAACTGATGGGGCCAAACAGGACTTTTCATACCGAAAATCCCTCAACAACTATTTGATGAAGAAATACCCTGACCGTGCTGAAGAATTCATTGACAAGTACTTTACGAAACCTCGGCCTAGCGGAAACAGAGACAGGAACAACCAAGATGCCACACCACCTGGGGAAGAACAGTCTCAGCCTCCGAATCAGTCTATAGGTAATGGAGGTGACGACTTTCAGACACAGACTCAGTCTCAGTCTCCATCTCAGACTCGGGCTCAGTCTCCATCTCAGGCTCAGGCTCAGTCTCCATCTCAGACGCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGTCTCCATCCCAGACTCAGACTCAGTCTCCGTCTCAGACTCAGGCTCAGGCTCAGTCTCCATCATCTCAGTCTCCGTCCCAGACGCAGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1976

Amino Acids

218.23

Weight (kDa)

5.88

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 46 - 218 7.4e-09 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 321 - 475 3.7e-35 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 478 - 624 4.5e-14 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 748 - 1171 1.6e-12 RNA polymerase Rpb1, domain 5
DUF3223 PF11523 1751 - 1826 3.1e-27 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5409
AasI GACNNNNNNGTC 2 cut(s) 2457, 4932
Acc16I TGCGCA 1 cut(s) 1038
Acc36I ACCTGC 3 cut(s) 1183, 4582, 5409
AccB1I GGYRCC 1 cut(s) 413
AccB7I CCANNNNNTGG 1 cut(s) 5069
AccBSI CCGCTC 1 cut(s) 1513
AccI GTMKAC 1 cut(s) 1101
AccII CGCG 4 cut(s) 517, 1727, 2628, 3654
AccIII TCCGGA 1 cut(s) 3177
AciI CCGC 9 cut(s) 105, 180, 344, 641, 854, 1513, 3196, 3590, 5559
AcoI YGGCCR 1 cut(s) 2809
AcuI CTGAAG 9 cut(s) 326, 452, 749, 813, 1724, 2045, 2096, 3387, 5538
AdeI CACNNNGTG 1 cut(s) 1996
AfaI GTAC 7 cut(s) 844, 3387, 3667, 3740, 4003, 5276, 5537
AfeI AGCGCT 2 cut(s) 1275, 5183
AfiI CCNNNNNNNGG 8 cut(s) 611, 838, 1774, 1881, 3201, 3202, 3754, 5069
AflIII ACRYGT 3 cut(s) 515, 1003, 3652
AhdI GACNNNNNGTC 1 cut(s) 4423
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 2539, 2571
AloI GAACNNNNNNTCC 4 cut(s) 420, 452, 2983, 3015
Alw21I GWGCWC 3 cut(s) 1416, 3687, 4466
Ama87I CYCGRG 3 cut(s) 1187, 3845, 5691
Aor13HI TCCGGA 1 cut(s) 3177
Aor51HI AGCGCT 2 cut(s) 1275, 5183
AoxI GGCC 6 cut(s) 1606, 2809, 4723, 5129, 5448, 5552
ArsI GACNNNNNNTTYG 6 cut(s) 764, 796, 4010, 4042, 5297, 5329
AseI ATTAAT 1 cut(s) 111
Asp700I GAANNNNTTC 1 cut(s) 2794
AspA2I CCTAGG 1 cut(s) 5126
AspLEI GCGC 5 cut(s) 1039, 1276, 2628, 4910, 5184
AsuC2I CCSGG 3 cut(s) 1188, 1189, 4022
AvaI CYCGRG 3 cut(s) 1187, 3845, 5691
AvaII GGWCC 7 cut(s) 1734, 1778, 4445, 4622, 4673, 4775, 5079
AvrII CCTAGG 1 cut(s) 5126
BaeGI GKGCMC 2 cut(s) 329, 1173
BaeI ACNNNNGTAYC 4 cut(s) 611, 644, 2721, 2754
BalI TGGCCA 1 cut(s) 2811
BamHI GGATCC 6 cut(s) 558, 697, 1020, 1160, 2248, 3174
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 5 cut(s) 1416, 3687, 3873, 4466, 5698
BarI GAAGNNNNNNTAC 4 cut(s) 603, 635, 2120, 2152
BbsI GAAGAC 5 cut(s) 1302, 1872, 2102, 2150, 4953
Bbv12I GWGCWC 3 cut(s) 1416, 3687, 4466
BbvCI CCTCAGC 1 cut(s) 1842
BceAI ACGGC 1 cut(s) 603
BciVI GTATCC 4 cut(s) 608, 814, 4756, 4948
BclI TGATCA 1 cut(s) 3108
BcnI CCSGG 3 cut(s) 1188, 1189, 4022
BfmI CTRYAG 9 cut(s) 455, 1634, 1811, 2401, 2733, 4323, 4794, 4941, 5631
BfoI RGCGCY 3 cut(s) 1277, 4911, 5185
BfuAI ACCTGC 3 cut(s) 1183, 4582, 5409
BfuI GTATCC 4 cut(s) 608, 814, 4756, 4948
BglI GCCNNNNNGGC 1 cut(s) 2808
BglII AGATCT 2 cut(s) 891, 5138
BlnI CCTAGG 1 cut(s) 5126
BlpI GCTNAGC 4 cut(s) 281, 1439, 2319, 3133
BmcAI AGTACT 1 cut(s) 5537
Bme18I GGWCC 7 cut(s) 1734, 1778, 4445, 4622, 4673, 4775, 5079
BmeRI GACNNNNNGTC 1 cut(s) 4423
BmeT110I CYCGRG 3 cut(s) 1187, 3845, 5691
BmrI ACTGGG 5 cut(s) 114, 3083, 3946, 4186, 4297
BmuI ACTGGG 5 cut(s) 114, 3083, 3946, 4186, 4297
BoxI GACNNNNGTC 2 cut(s) 3282, 5315
BpiI GAAGAC 5 cut(s) 1302, 1872, 2102, 2150, 4953
BpmI CTGGAG 7 cut(s) 815, 1008, 1529, 1790, 2493, 3519, 5342
Bpu10I CCTNAGC 4 cut(s) 1395, 1842, 5715, 5883
Bpu1102I GCTNAGC 4 cut(s) 281, 1439, 2319, 3133
BpuEI CTTGAG 4 cut(s) 591, 1113, 1966, 4083
BpuMI CCSGG 3 cut(s) 1188, 1189, 4022
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 5 cut(s) 825, 2054, 2868, 3045, 4974
BsaI GGTCTC 3 cut(s) 3421, 4991, 5280
BsaWI WCCGGW 1 cut(s) 3177
Bsc4I CCNNNNNNNGG 8 cut(s) 611, 838, 1774, 1881, 3201, 3202, 3754, 5069
Bse3DI GCAATG 1 cut(s) 2831
Bse8I GATNNNNATC 5 cut(s) 825, 2054, 2868, 3045, 4974
BseAI TCCGGA 1 cut(s) 3177
BseCI ATCGAT 1 cut(s) 92
BseJI GATNNNNATC 5 cut(s) 825, 2054, 2868, 3045, 4974
BseLI CCNNNNNNNGG 8 cut(s) 611, 838, 1774, 1881, 3201, 3202, 3754, 5069
BseMI GCAATG 1 cut(s) 2831
BseRI GAGGAG 1 cut(s) 5219
BseSI GKGCMC 2 cut(s) 329, 1173
BseYI CCCAGC 1 cut(s) 1609
BsgI GTGCAG 2 cut(s) 2108, 5115
Bsh1236I CGCG 4 cut(s) 517, 1727, 2628, 3654
BshFI GGCC 6 cut(s) 1608, 2811, 4725, 5131, 5450, 5554
BshNI GGYRCC 1 cut(s) 413
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 3 cut(s) 1416, 3687, 4466
BsiHKCI CYCGRG 3 cut(s) 1187, 3845, 5691
BsiSI CCGG 3 cut(s) 1188, 3178, 4022
BslI CCNNNNNNNGG 8 cut(s) 611, 838, 1774, 1881, 3201, 3202, 3754, 5069
BsmBI CGTCTC 1 cut(s) 5874
BsmI GAATGC 2 cut(s) 137, 2654
BsnI GGCC 6 cut(s) 1608, 2811, 4725, 5131, 5450, 5554
Bso31I GGTCTC 3 cut(s) 3421, 4991, 5280
BsoBI CYCGRG 3 cut(s) 1187, 3845, 5691
Bsp1286I GDGCHC 7 cut(s) 329, 1173, 1416, 3687, 3873, 4466, 5698
Bsp13I TCCGGA 1 cut(s) 3177
Bsp1407I TGTACA 1 cut(s) 3738
Bsp1720I GCTNAGC 4 cut(s) 281, 1439, 2319, 3133
BspACI CCGC 9 cut(s) 105, 180, 344, 641, 854, 1513, 3196, 3590, 5559
BspANI GGCC 6 cut(s) 1608, 2811, 4725, 5131, 5450, 5554
BspDI ATCGAT 1 cut(s) 92
BspEI TCCGGA 1 cut(s) 3177
BspFNI CGCG 4 cut(s) 517, 1727, 2628, 3654
BspHI TCATGA 2 cut(s) 1291, 5238
BspMAI CTGCAG 4 cut(s) 459, 1815, 2737, 4945
BspMI ACCTGC 3 cut(s) 1183, 4582, 5409
BspQI GCTCTTC 4 cut(s) 1421, 2308, 3955, 4454
BspT107I GGYRCC 1 cut(s) 413
BspTNI GGTCTC 3 cut(s) 3421, 4991, 5280
BsrBI CCGCTC 1 cut(s) 1513
BsrDI GCAATG 1 cut(s) 2831
BsrGI TGTACA 1 cut(s) 3738
BssT1I CCWWGG 3 cut(s) 1164, 3420, 5126
Bst6I CTCTTC 7 cut(s) 973, 1272, 1421, 2253, 2308, 3955, 4454
BstAPI GCANNNNNTGC 3 cut(s) 2501, 3352, 5215
BstAUI TGTACA 1 cut(s) 3738
BstC8I GCNNGC 6 cut(s) 528, 1641, 2689, 3381, 3920, 4906
BstDSI CCRYGG 1 cut(s) 4979
BstEII GGTNACC 1 cut(s) 147
BstENI CCTNNNNNAGG 2 cut(s) 609, 1772
BstFNI CGCG 4 cut(s) 517, 1727, 2628, 3654
BstH2I RGCGCY 3 cut(s) 1277, 4911, 5185
BstHHI GCGC 5 cut(s) 1039, 1276, 2628, 4910, 5184
BstNSI RCATGY 1 cut(s) 1007
BstPAI GACNNNNGTC 2 cut(s) 3282, 5315
BstPI GGTNACC 1 cut(s) 147
BstSFI CTRYAG 9 cut(s) 455, 1634, 1811, 2401, 2733, 4323, 4794, 4941, 5631
BstSLI GKGCMC 2 cut(s) 329, 1173
BstUI CGCG 4 cut(s) 517, 1727, 2628, 3654
BstV2I GAAGAC 5 cut(s) 1302, 1872, 2102, 2150, 4953
BstXI CCANNNNNNTGG 2 cut(s) 694, 4785
Bsu15I ATCGAT 1 cut(s) 92
BsuI GTATCC 4 cut(s) 608, 814, 4756, 4948
BsuRI GGCC 6 cut(s) 1608, 2811, 4725, 5131, 5450, 5554
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 4979
BtgZI GCGATG 2 cut(s) 1187, 1742
BtsI GCAGTG 4 cut(s) 2535, 2742, 3976, 4938
BveI ACCTGC 3 cut(s) 1183, 4582, 5409
Cac8I GCNNGC 6 cut(s) 528, 1641, 2689, 3381, 3920, 4906
CciI TCATGA 2 cut(s) 1291, 5238
CfoI GCGC 5 cut(s) 1039, 1276, 2628, 4910, 5184
Cfr9I CCCGGG 1 cut(s) 1187
ClaI ATCGAT 1 cut(s) 92
CseI GACGC 5 cut(s) 506, 967, 1733, 3380, 5746
CsiI ACCWGGT 1 cut(s) 4669
Csp6I GTAC 7 cut(s) 843, 3386, 3666, 3739, 4002, 5275, 5536
CspCI CAANNNNNGTGG 4 cut(s) 3232, 3267, 5071, 5106
CviQI GTAC 7 cut(s) 843, 3386, 3666, 3739, 4002, 5275, 5536
DraI TTTAAA 1 cut(s) 2643
DraIII CACNNNGTG 1 cut(s) 1996
DrdI GACNNNNNNGTC 2 cut(s) 2457, 4932
DriI GACNNNNNGTC 1 cut(s) 4423
DseDI GACNNNNNNGTC 2 cut(s) 2457, 4932
EaeI YGGCCR 1 cut(s) 2809
Eam1104I CTCTTC 7 cut(s) 973, 1272, 1421, 2253, 2308, 3955, 4454
Eam1105I GACNNNNNGTC 1 cut(s) 4423
EarI CTCTTC 7 cut(s) 973, 1272, 1421, 2253, 2308, 3955, 4454
EciI GGCGGA 1 cut(s) 359
Ecl136II GAGCTC 3 cut(s) 1414, 3685, 4464
Eco130I CCWWGG 3 cut(s) 1164, 3420, 5126
Eco24I GRGCYC 5 cut(s) 1416, 3687, 3873, 4466, 5698
Eco31I GGTCTC 3 cut(s) 3421, 4991, 5280
Eco32I GATATC 2 cut(s) 2056, 2365
Eco47I GGWCC 7 cut(s) 1734, 1778, 4445, 4622, 4673, 4775, 5079
Eco47III AGCGCT 2 cut(s) 1275, 5183
Eco53kI GAGCTC 3 cut(s) 1414, 3685, 4464
Eco57I CTGAAG 9 cut(s) 326, 452, 749, 813, 1724, 2045, 2096, 3387, 5538
Eco88I CYCGRG 3 cut(s) 1187, 3845, 5691
Eco91I GGTNACC 1 cut(s) 147
EcoICRI GAGCTC 3 cut(s) 1414, 3685, 4464
EcoNI CCTNNNNNAGG 2 cut(s) 609, 1772
EcoO109I RGGNCCY 4 cut(s) 4445, 4622, 4673, 4724
EcoO65I GGTNACC 1 cut(s) 147
EcoRI GAATTC 5 cut(s) 2741, 4534, 4603, 4705, 5522
EcoRV GATATC 2 cut(s) 2056, 2365
EcoT14I CCWWGG 3 cut(s) 1164, 3420, 5126
EcoT22I ATGCAT 4 cut(s) 3193, 3262, 3784, 5265
EcoT38I GRGCYC 5 cut(s) 1416, 3687, 3873, 4466, 5698
ErhI CCWWGG 3 cut(s) 1164, 3420, 5126
Esp3I CGTCTC 1 cut(s) 5874
FalI AAGNNNNNCTT 6 cut(s) 2216, 2248, 2706, 2738, 3339, 3371
FauI CCCGC 3 cut(s) 648, 1506, 3203
FbaI TGATCA 1 cut(s) 3108
FblI GTMKAC 1 cut(s) 1101
FriOI GRGCYC 5 cut(s) 1416, 3687, 3873, 4466, 5698
FspI TGCGCA 1 cut(s) 1038
GlaI GCGC 5 cut(s) 1038, 1275, 2627, 4909, 5183
GsaI CCCAGC 1 cut(s) 1613
GsuI CTGGAG 7 cut(s) 815, 1008, 1529, 1790, 2493, 3519, 5342
HaeII RGCGCY 3 cut(s) 1277, 4911, 5185
HaeIII GGCC 6 cut(s) 1608, 2811, 4725, 5131, 5450, 5554
HapII CCGG 3 cut(s) 1188, 3178, 4022
HgaI GACGC 5 cut(s) 506, 967, 1733, 3380, 5746
HhaI GCGC 5 cut(s) 1039, 1276, 2628, 4910, 5184
Hin6I GCGC 5 cut(s) 1037, 1274, 2626, 4908, 5182
HinP1I GCGC 5 cut(s) 1037, 1274, 2626, 4908, 5182
HincII GTYRAC 3 cut(s) 125, 1723, 5377
HindII GTYRAC 3 cut(s) 125, 1723, 5377
HindIII AAGCTT 4 cut(s) 375, 915, 3510, 5360
HpaII CCGG 3 cut(s) 1188, 3178, 4022
Hpy166II GTNNAC 9 cut(s) 125, 1072, 1102, 1723, 2450, 3085, 3739, 5166, 5377
Hpy8I GTNNAC 9 cut(s) 125, 1072, 1102, 1723, 2450, 3085, 3739, 5166, 5377
HpyCH4IV ACGT 8 cut(s) 723, 1654, 2446, 3020, 3170, 4239, 4419, 5312
HpySE526I ACGT 8 cut(s) 723, 1654, 2446, 3020, 3170, 4239, 4419, 5312
HspAI GCGC 5 cut(s) 1037, 1274, 2626, 4908, 5182
Kpn2I TCCGGA 1 cut(s) 3177
Ksp22I TGATCA 1 cut(s) 3108
LguI GCTCTTC 4 cut(s) 1421, 2308, 3955, 4454
MabI ACCWGGT 1 cut(s) 4669
MaeII ACGT 8 cut(s) 723, 1654, 2446, 3020, 3170, 4239, 4419, 5312
MbiI CCGCTC 1 cut(s) 1513
MfeI CAATTG 1 cut(s) 2274
MhlI GDGCHC 7 cut(s) 329, 1173, 1416, 3687, 3873, 4466, 5698
MlsI TGGCCA 1 cut(s) 2811
MluI ACGCGT 2 cut(s) 515, 3652
MluNI TGGCCA 1 cut(s) 2811
MmeI TCCRAC 3 cut(s) 25, 2464, 4058
Mox20I TGGCCA 1 cut(s) 2811
Mph1103I ATGCAT 4 cut(s) 3193, 3262, 3784, 5265
MroI TCCGGA 1 cut(s) 3177
MroXI GAANNNNTTC 1 cut(s) 2794
MscI TGGCCA 1 cut(s) 2811
MslI CAYNNNNRTG 5 cut(s) 261, 692, 869, 1008, 2855
Msp20I TGGCCA 1 cut(s) 2811
MspA1I CMGCKG 2 cut(s) 1459, 4781
MspI CCGG 3 cut(s) 1188, 3178, 4022
MunI CAATTG 1 cut(s) 2274
Mva1269I GAATGC 2 cut(s) 137, 2654
MvnI CGCG 4 cut(s) 517, 1727, 2628, 3654
NciI CCSGG 3 cut(s) 1188, 1189, 4022
NmeAIII GCCGAG 2 cut(s) 3224, 5530
NmuCI GTSAC 5 cut(s) 68, 3220, 4502, 4820, 5645
NsbI TGCGCA 1 cut(s) 1038
NsiI ATGCAT 4 cut(s) 3193, 3262, 3784, 5265
NspI RCATGY 1 cut(s) 1007
PagI TCATGA 2 cut(s) 1291, 5238
PaqCI CACCTGC 1 cut(s) 5409
PciI ACATGT 1 cut(s) 1003
PciSI GCTCTTC 4 cut(s) 1421, 2308, 3955, 4454
PctI GAATGC 2 cut(s) 137, 2654
PdmI GAANNNNTTC 1 cut(s) 2794
PflFI GACNNNGTC 3 cut(s) 5668, 5740, 5860
PflMI CCANNNNNTGG 1 cut(s) 5069
PfoI TCCNGGA 3 cut(s) 477, 1767, 5319
PpuMI RGGWCCY 3 cut(s) 4445, 4622, 4673
PscI ACATGT 1 cut(s) 1003
PshAI GACNNNNGTC 2 cut(s) 3282, 5315
PshBI ATTAAT 1 cut(s) 111
Psp124BI GAGCTC 3 cut(s) 1416, 3687, 4466
Psp5II RGGWCCY 3 cut(s) 4445, 4622, 4673
PspEI GGTNACC 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 1609
PspPPI RGGWCCY 3 cut(s) 4445, 4622, 4673
PstI CTGCAG 4 cut(s) 459, 1815, 2737, 4945
PsyI GACNNNGTC 3 cut(s) 5668, 5740, 5860
PvuII CAGCTG 2 cut(s) 1459, 4781
RsaI GTAC 7 cut(s) 844, 3387, 3667, 3740, 4003, 5276, 5537
RsaNI GTAC 7 cut(s) 843, 3386, 3666, 3739, 4002, 5275, 5536
RseI CAYNNNNRTG 5 cut(s) 261, 692, 869, 1008, 2855
SacI GAGCTC 3 cut(s) 1416, 3687, 4466
SapI GCTCTTC 4 cut(s) 1421, 2308, 3955, 4454
ScaI AGTACT 1 cut(s) 5537
SduI GDGCHC 7 cut(s) 329, 1173, 1416, 3687, 3873, 4466, 5698
SexAI ACCWGGT 1 cut(s) 4669
SfcI CTRYAG 9 cut(s) 455, 1634, 1811, 2401, 2733, 4323, 4794, 4941, 5631
SinI GGWCC 7 cut(s) 1734, 1778, 4445, 4622, 4673, 4775, 5079
SmaI CCCGGG 1 cut(s) 1189
SmiMI CAYNNNNRTG 5 cut(s) 261, 692, 869, 1008, 2855
SmlI CTYRAG 4 cut(s) 606, 1128, 1945, 4098
SmoI CTYRAG 4 cut(s) 606, 1128, 1945, 4098
SsiI CCGC 9 cut(s) 105, 180, 344, 641, 854, 1513, 3196, 3590, 5559
SstI GAGCTC 3 cut(s) 1416, 3687, 4466
StyI CCWWGG 3 cut(s) 1164, 3420, 5126
TaiI ACGT 8 cut(s) 726, 1657, 2449, 3023, 3173, 4242, 4422, 5315
TaqI TCGA 2 cut(s) 92, 2751
TatI WGTACW 4 cut(s) 3385, 3738, 4001, 5535
TauI GCSGC 2 cut(s) 857, 3593
TseFI GTSAC 5 cut(s) 68, 3220, 4502, 4820, 5645
Tsp45I GTSAC 5 cut(s) 68, 3220, 4502, 4820, 5645
TspGWI ACGGA 9 cut(s) 48, 571, 938, 2241, 3139, 4968, 5256, 5856, 5901
TspMI CCCGGG 1 cut(s) 1187
Tth111I GACNNNGTC 3 cut(s) 5668, 5740, 5860
Van91I CCANNNNNTGG 1 cut(s) 5069
VpaK11BI GGWCC 7 cut(s) 1734, 1778, 4445, 4622, 4673, 4775, 5079
VspI ATTAAT 1 cut(s) 111
XagI CCTNNNNNAGG 2 cut(s) 609, 1772
XbaI TCTAGA 3 cut(s) 424, 3049, 3712
XceI RCATGY 1 cut(s) 1007
XcmI CCANNNNNNNNNTGG 2 cut(s) 2426, 3704
XmaI CCCGGG 1 cut(s) 1187
XmaJI CCTAGG 1 cut(s) 5126
XmiI GTMKAC 1 cut(s) 1101
XmnI GAANNNNTTC 1 cut(s) 2794
ZrmI AGTACT 1 cut(s) 5537
Zsp2I ATGCAT 4 cut(s) 3193, 3262, 3784, 5265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.