pycom02g24380

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006658
Physical Location & Seq
Reverse (-)
22509123 .. 22513716
4594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24380.1

Sequence Viewer

Length: 2082 bp
ATGTCTGCGGATCCTTCAATTTCAATGCTCAGAAAAGTTCTAAAGCAAATTGAAGTCATAAGAAGTTCAAGGTCAGGCATCCCAAACTTTGAATCACAAATTGTTGAAGCTAATGAACTTCAAGCCATAGTGGATCAGTATCTTCAAGTTAGGGGTACTGGGAAGGCATCACGGGATATAGATGCAAGATTTGGGGTAAACAAGGAGCTGAATGCCTCTTCCACTAAAGCATGGCTTGAAAAAATGAGAACACTGTTCATTAGAAAGGGTTCAGGATTCTCTTCTCGCTCAGTAATCACTGGGGATGCCTTTAGAAGGGTAAATGAAGTTGGCATCCCCTATGAAATTGCTCAAAGAATCACCTTCGAGGAGAGGGTTAATGATCATAACATTAGGTACTTACAACAGCTGGTAGATAGCAAGTTGTGCCTAACCTATAGGGATCGATCATCAACTTACTCACTTAGGGAAGGTTCAAAGGGGCACACTTTTTTGAGGCCTGGTCAAGTGGTGCATCGGAGGATTATGGATGGGGACCTTGTATTTGTAAACAGGCCCCCAACTACCCATAAACATTCCCTACAAGCACTACAAGTGTATGTACATGATGACCATGTGGTGAAAATAAATCCTTTGATATGTGGGCCACTAAGTGCTGACTTTGATGGTGATTGCATCCATTTGTTCTATCCGCAATCGCTTGCTGCAAAAGCAGAAGTTTTGGAGCTTTTTTCAGTGGAGAAGCAGCTGCTTAGCTCTCATAGTGGAAAACCAAATCTGCAAATGGCCACTGATTCACTATTGTCATTGAGGATGATGTTTAAGAACTATTTTTTGGATAAATCAGCAGCTCAGCAACTGGCCATGTTTGCTTCATCTTTGTTGCCACGACCTGCATTATTGAAAGCAAACTCTGCTTGCTCATATTGGACTGCTTTGCAGATATTACAGACCGCATTGCCTCCTGACTTTGACTACTGTGGGGAAAAGTACTTGGTCAATAAAAGTGAGATACTGAAAATTGAGTTCAGTACTAGTGCAGTTTCAGCGGTGATGAATGACATTGCTACTTCAGTTTTCTTTGAGAAAGGGGGCGAAGATGTCCTTAAATTCTTTGATTCTCTACAGCCCTTGCTGATGGAGAATATATTTTCAGAAGGGTTCAGTGTTGGCTTGGAGGATTTTTACATGTCAAAGACATCCATACAAGATATTAAGGAGAATATTCAGGGTACTTCAGATTTGTTGTATCATCTGAGGTCAACATACAATGAATTTGTAGAGTTTCAGCTGGAGAGTCGAATCAAAAGCTTGAAAGTACCAGTTTCAAATTTTATCTTGGAGTCATCTGCATTCGGTGATTTAATCGACACCAAGAGTGATTCGGCAATCAACAAAGTTGTACAACAAATTGGGTTCCTAGGCCTACAACTCTCTGATAAGGGAAGATTCTACTCAAAGACATTGGTTGAGGATGTGGCCTCTCTCTGTCACAACAAATATTCTTTTAACACGGAGTATCCCTCTGCAGAATATGGCCTAGTTCAAAGTTGTTTCTTCCATGGGTTAGATCCATACGAGGAAATAGTTCACTCCATTGCTACACGAGAGGTGATTGTTCGGTCCTCAAGAGGATTGACTGAACCTGGGACCTTATTTAAAAATTTAATGGCCATTCTTCGTGATGTGGTTATTTGCTATGATGGCACGGTTAGAAATGTTTGTAGCAATTCAGTTATCCAATTTGAGTATGGAGTCAATGCTGGATCTAGGCCCCCGAATCTTTTCCCTGCTGGTGAACCTGTTGGCGTGTTAGCTGCCACTGCAATGTCAAATCCTGCGTACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCTTCATGGGAGCTGATGAAGGAAATTCTACTATGCAAAGTAAATTTTAAGAATGAGCTCATTGATCGCCGTGTAATTTTGTACTTGAATAACTGTGGCTGTGGAAGAAAGTACTGCCGAGAACGTGCAGCATGTTTGGTTAAGAATCAGTTGAAGAAAGTCAGTCTTAAAGATACTGCAGTCGAATTCATCGATCGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

694

Amino Acids

77.82

Weight (kDa)

7.51

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_2 PF00623 92 - 246 8e-34 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 249 - 394 2.3e-15 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 522 - 615 2.6e-06 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
AciI CCGC 4 cut(s) 8, 692, 954, 1049
AclWI GGATC 6 cut(s) 5, 18, 141, 450, 1565, 1774
AcoI YGGCCR 3 cut(s) 786, 861, 1671
AcsI RAATTY 7 cut(s) 1109, 1274, 1330, 1663, 1902, 1921, 2063
AcuI CTGAAG 2 cut(s) 1056, 1221
AdeI CACNNNGTG 1 cut(s) 653
AfiI CCNNNNNNNGG 1 cut(s) 315
AflIII ACRYGT 1 cut(s) 1188
AhlI ACTAGT 1 cut(s) 1034
AjnI CCWGG 2 cut(s) 499, 1645
AjuI GAANNNNNNNTTGG 2 cut(s) 818, 850
Alw21I GWGCWC 1 cut(s) 1938
AlwI GGATC 6 cut(s) 5, 18, 141, 450, 1565, 1774
AlwNI CAGNNNCTG 1 cut(s) 859
ApeKI GCWGC 7 cut(s) 704, 745, 748, 848, 1817, 1872, 2006
ApoI RAATTY 7 cut(s) 1109, 1274, 1330, 1663, 1902, 1921, 2063
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 3 cut(s) 268, 1587, 1784
AspA2I CCTAGG 1 cut(s) 1420
AspS9I GGNCC 6 cut(s) 535, 555, 644, 1623, 1650, 1773
AsuHPI GGTGA 7 cut(s) 352, 631, 680, 1063, 1370, 1624, 1808
AvaII GGWCC 3 cut(s) 535, 1623, 1650
AvrII CCTAGG 1 cut(s) 1420
BaeGI GKGCMC 1 cut(s) 486
BaeI ACNNNNGTAYC 2 cut(s) 2043, 2076
BalI TGGCCA 3 cut(s) 788, 863, 1673
BamHI GGATCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 1938
BauI CACGAG 1 cut(s) 1605
Bbv12I GWGCWC 1 cut(s) 1938
BbvI GCAGC 7 cut(s) 691, 735, 757, 860, 1804, 1884, 2018
BccI CCATC 4 cut(s) 524, 659, 1132, 1697
BceAI ACGGC 1 cut(s) 1932
BciT130I CCWGG 2 cut(s) 501, 1647
BciVI GTATCC 1 cut(s) 1530
BclI TGATCA 1 cut(s) 382
BcuI ACTAGT 1 cut(s) 1034
BfaI CTAG 4 cut(s) 1035, 1421, 1541, 1770
BfmI CTRYAG 4 cut(s) 436, 1124, 1527, 2055
BfuAI ACCTGC 1 cut(s) 901
BfuI GTATCC 1 cut(s) 1530
BisI GCNGC 7 cut(s) 705, 746, 749, 849, 1818, 1873, 2007
BlnI CCTAGG 1 cut(s) 1420
BlpI GCTNAGC 2 cut(s) 752, 852
BlsI GCNGC 7 cut(s) 706, 747, 750, 850, 1819, 1874, 2008
BmcAI AGTACT 3 cut(s) 992, 1033, 1991
Bme1390I CCNGG 2 cut(s) 501, 1647
Bme18I GGWCC 3 cut(s) 535, 1623, 1650
BmgT120I GGNCC 6 cut(s) 535, 555, 644, 1623, 1650, 1773
BmiI GGNNCC 6 cut(s) 12, 536, 557, 1418, 1651, 1775
BmrFI CCNGG 2 cut(s) 501, 1647
BmrI ACTGGG 2 cut(s) 168, 309
BmsI GCATC 7 cut(s) 87, 172, 176, 295, 342, 523, 684
BmuI ACTGGG 2 cut(s) 168, 309
BplI GAGNNNNNCTC 2 cut(s) 1508, 1540
BpmI CTGGAG 1 cut(s) 1313
Bpu1102I GCTNAGC 2 cut(s) 752, 852
BpuEI CTTGAG 1 cut(s) 1612
Bsa29I ATCGAT 2 cut(s) 445, 2070
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 1420, 1561, 1646
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse1I ACTGG 4 cut(s) 163, 304, 864, 1322
Bse3DI GCAATG 4 cut(s) 956, 1062, 1596, 1833
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 2 cut(s) 501, 1647
BseCI ATCGAT 2 cut(s) 445, 2070
BseDI CCNNGG 3 cut(s) 1420, 1561, 1646
BseGI GGATG 8 cut(s) 78, 310, 333, 535, 675, 819, 1199, 1480
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMI GCAATG 4 cut(s) 956, 1062, 1596, 1833
BseMII CTCAG 4 cut(s) 43, 303, 866, 1247
BseNI ACTGG 4 cut(s) 163, 304, 864, 1322
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 7 cut(s) 691, 735, 757, 860, 1804, 1884, 2018
BsgI GTGCAG 2 cut(s) 1059, 2025
Bsh1285I CGRYCG 1 cut(s) 2074
BshVI ATCGAT 2 cut(s) 445, 2070
BsiEI CGRYCG 1 cut(s) 2074
BsiHKAI GWGCWC 1 cut(s) 1938
BslFI GGGAC 2 cut(s) 548, 1663
BslI CCNNNNNNNGG 1 cut(s) 315
BsmFI GGGAC 2 cut(s) 548, 1663
BsmI GAATGC 2 cut(s) 217, 1352
Bsp1286I GDGCHC 2 cut(s) 486, 1938
Bsp1407I TGTACA 2 cut(s) 601, 1402
Bsp143I GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
Bsp1720I GCTNAGC 2 cut(s) 752, 852
Bsp19I CCATGG 1 cut(s) 1561
BspACI CCGC 4 cut(s) 8, 692, 954, 1049
BspCNI CTCAG 4 cut(s) 42, 302, 865, 1248
BspDI ATCGAT 2 cut(s) 445, 2070
BspLI GGNNCC 6 cut(s) 12, 536, 557, 1418, 1651, 1775
BspMAI CTGCAG 2 cut(s) 1531, 2059
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 6 cut(s) 5, 18, 141, 450, 1565, 1774
BsrDI GCAATG 4 cut(s) 956, 1062, 1596, 1833
BsrGI TGTACA 2 cut(s) 601, 1402
BsrI ACTGG 4 cut(s) 163, 304, 864, 1322
BssECI CCNNGG 3 cut(s) 1420, 1561, 1646
BssMI GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
BssSI CACGAG 1 cut(s) 1605
BssT1I CCWWGG 2 cut(s) 1420, 1561
Bst2BI CACGAG 1 cut(s) 1605
Bst2UI CCWGG 2 cut(s) 501, 1647
Bst4CI ACNGT 4 cut(s) 255, 980, 1711, 1973
Bst6I CTCTTC 3 cut(s) 223, 286, 1885
BstAPI GCANNNNNTGC 2 cut(s) 426, 914
BstAUI TGTACA 2 cut(s) 601, 1402
BstC8I GCNNGC 2 cut(s) 702, 919
BstDEI CTNAG 7 cut(s) 29, 289, 464, 650, 752, 852, 1256
BstDSI CCRYGG 1 cut(s) 1561
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 8 cut(s) 78, 310, 333, 535, 675, 819, 1199, 1480
BstKTI GATC 9 cut(s) 13, 136, 385, 445, 449, 1573, 1769, 1945, 2074
BstMBI GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
BstMCI CGRYCG 1 cut(s) 2074
BstMWI GCNNNNNNNGC 8 cut(s) 426, 710, 869, 914, 1046, 1704, 1823, 1847
BstNI CCWGG 2 cut(s) 501, 1647
BstNSI RCATGY 2 cut(s) 1192, 2013
BstSCI CCNGG 2 cut(s) 499, 1645
BstSFI CTRYAG 4 cut(s) 436, 1124, 1527, 2055
BstSLI GKGCMC 1 cut(s) 486
BstV1I GCAGC 7 cut(s) 691, 735, 757, 860, 1804, 1884, 2018
BstX2I RGATCY 3 cut(s) 10, 1570, 1766
BstYI RGATCY 3 cut(s) 10, 1570, 1766
Bsu15I ATCGAT 2 cut(s) 445, 2070
BsuI GTATCC 1 cut(s) 1530
BsuTUI ATCGAT 2 cut(s) 445, 2070
BtgI CCRYGG 1 cut(s) 1561
BtsCI GGATG 8 cut(s) 78, 310, 333, 535, 675, 819, 1199, 1480
BtsI GCAGTG 1 cut(s) 1821
BtsIMutI CAGTG 6 cut(s) 251, 297, 741, 789, 1171, 1821
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 2 cut(s) 702, 919
CaiI CAGNNNCTG 1 cut(s) 859
Cfr13I GGNCC 6 cut(s) 535, 555, 644, 1623, 1650, 1773
ClaI ATCGAT 2 cut(s) 445, 2070
CviAII CATG 8 cut(s) 231, 605, 614, 865, 1189, 1562, 1884, 2010
DdeI CTNAG 7 cut(s) 29, 289, 464, 650, 752, 852, 1256
DpnI GATC 9 cut(s) 12, 135, 384, 444, 448, 1572, 1768, 1944, 2073
DpnII GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
DraI TTTAAA 1 cut(s) 1660
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 3 cut(s) 786, 861, 1671
Eam1104I CTCTTC 3 cut(s) 223, 286, 1885
EarI CTCTTC 3 cut(s) 223, 286, 1885
Ecl136II GAGCTC 1 cut(s) 1936
Eco130I CCWWGG 2 cut(s) 1420, 1561
Eco147I AGGCCT 2 cut(s) 499, 1425
Eco24I GRGCYC 1 cut(s) 1938
Eco47I GGWCC 3 cut(s) 535, 1623, 1650
Eco53kI GAGCTC 1 cut(s) 1936
Eco57I CTGAAG 2 cut(s) 1056, 1221
EcoICRI GAGCTC 1 cut(s) 1936
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoO109I RGGNCCY 4 cut(s) 535, 555, 1650, 1773
EcoRI GAATTC 1 cut(s) 2063
EcoRII CCWGG 2 cut(s) 499, 1645
EcoT14I CCWWGG 2 cut(s) 1420, 1561
EcoT38I GRGCYC 1 cut(s) 1938
ErhI CCWWGG 2 cut(s) 1420, 1561
FaeI CATG 8 cut(s) 234, 608, 617, 868, 1192, 1565, 1887, 2013
FalI AAGNNNNNCTT 6 cut(s) 219, 251, 1089, 1121, 2028, 2060
FaqI GGGAC 2 cut(s) 548, 1663
FatI CATG 8 cut(s) 230, 604, 613, 864, 1188, 1561, 1883, 2009
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 7 cut(s) 705, 746, 749, 849, 1818, 1873, 2007
FokI GGATG 8 cut(s) 65, 317, 320, 542, 662, 826, 1186, 1487
FriOI GRGCYC 1 cut(s) 1938
Fsp4HI GCNGC 7 cut(s) 705, 746, 749, 849, 1818, 1873, 2007
FspBI CTAG 4 cut(s) 1035, 1421, 1541, 1770
GluI GCNGC 7 cut(s) 705, 746, 749, 849, 1818, 1873, 2007
GsuI CTGGAG 1 cut(s) 1313
Hin1II CATG 8 cut(s) 234, 608, 617, 868, 1192, 1565, 1887, 2013
HincII GTYRAC 1 cut(s) 1263
HindII GTYRAC 1 cut(s) 1263
HindIII AAGCTT 1 cut(s) 1309
HphI GGTGA 7 cut(s) 352, 631, 680, 1063, 1370, 1624, 1808
Hpy166II GTNNAC 5 cut(s) 199, 550, 1263, 1591, 1799
Hpy188I TCNGA 6 cut(s) 32, 519, 1156, 1240, 1257, 1438
Hpy188III TCNNGA 5 cut(s) 273, 965, 1629, 1682, 1856
Hpy8I GTNNAC 5 cut(s) 199, 550, 1263, 1591, 1799
HpyAV CCTTC 7 cut(s) 24, 157, 309, 373, 464, 1151, 1891
HpyCH4III ACNGT 4 cut(s) 255, 980, 1711, 1973
HpyCH4IV ACGT 1 cut(s) 2002
HpyF10VI GCNNNNNNNGC 8 cut(s) 426, 710, 869, 914, 1046, 1704, 1823, 1847
HpyF3I CTNAG 7 cut(s) 29, 289, 464, 650, 752, 852, 1256
HpySE526I ACGT 1 cut(s) 2002
Hsp92II CATG 8 cut(s) 234, 608, 617, 868, 1192, 1565, 1887, 2013
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
LmnI GCTCC 3 cut(s) 205, 724, 1888
Lsp1109I GCAGC 7 cut(s) 691, 735, 757, 860, 1804, 1884, 2018
LweI GCATC 7 cut(s) 87, 172, 176, 295, 342, 523, 684
MaeI CTAG 4 cut(s) 1035, 1421, 1541, 1770
MaeII ACGT 1 cut(s) 2002
MaeIII GTNAC 1 cut(s) 1490
MalI GATC 9 cut(s) 12, 135, 384, 444, 448, 1572, 1768, 1944, 2073
MboI GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
MflI RGATCY 3 cut(s) 10, 1570, 1766
MhlI GDGCHC 2 cut(s) 486, 1938
MlsI TGGCCA 3 cut(s) 788, 863, 1673
MluNI TGGCCA 3 cut(s) 788, 863, 1673
MlyI GAGTC 3 cut(s) 1306, 1352, 1764
Mox20I TGGCCA 3 cut(s) 788, 863, 1673
MroXI GAANNNNTTC 3 cut(s) 268, 1587, 1784
MscI TGGCCA 3 cut(s) 788, 863, 1673
MslI CAYNNNNRTG 1 cut(s) 1826
Msp20I TGGCCA 3 cut(s) 788, 863, 1673
MspA1I CMGCKG 4 cut(s) 409, 748, 1049, 1291
MspR9I CCNGG 2 cut(s) 501, 1647
Mva1269I GAATGC 2 cut(s) 217, 1352
MvaI CCWGG 2 cut(s) 501, 1647
MwoI GCNNNNNNNGC 8 cut(s) 426, 710, 869, 914, 1046, 1704, 1823, 1847
NcoI CCATGG 1 cut(s) 1561
NdeII GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
NlaIII CATG 8 cut(s) 234, 608, 617, 868, 1192, 1565, 1887, 2013
NlaIV GGNNCC 6 cut(s) 12, 536, 557, 1418, 1651, 1775
NmeAIII GCCGAG 1 cut(s) 2021
NmuCI GTSAC 1 cut(s) 1490
NspI RCATGY 2 cut(s) 1192, 2013
PceI AGGCCT 2 cut(s) 499, 1425
PciI ACATGT 1 cut(s) 1188
PcsI WCGNNNNNNNCGW 1 cut(s) 2067
PctI GAATGC 2 cut(s) 217, 1352
PdmI GAANNNNTTC 3 cut(s) 268, 1587, 1784
PkrI GCNGC 7 cut(s) 706, 747, 750, 850, 1819, 1874, 2008
Ple19I CGATCG 1 cut(s) 2074
PleI GAGTC 3 cut(s) 1305, 1351, 1763
PpsI GAGTC 3 cut(s) 1305, 1351, 1763
PpuMI RGGWCCY 2 cut(s) 535, 1650
PscI ACATGT 1 cut(s) 1188
Psp124BI GAGCTC 1 cut(s) 1938
Psp5II RGGWCCY 2 cut(s) 535, 1650
Psp6I CCWGG 2 cut(s) 499, 1645
PspGI CCWGG 2 cut(s) 499, 1645
PspN4I GGNNCC 6 cut(s) 12, 536, 557, 1418, 1651, 1775
PspPI GGNCC 6 cut(s) 535, 555, 644, 1623, 1650, 1773
PspPPI RGGWCCY 2 cut(s) 535, 1650
PstI CTGCAG 2 cut(s) 1531, 2059
PstNI CAGNNNCTG 1 cut(s) 859
PsuI RGATCY 3 cut(s) 10, 1570, 1766
PvuI CGATCG 1 cut(s) 2074
PvuII CAGCTG 3 cut(s) 409, 748, 1291
RseI CAYNNNNRTG 1 cut(s) 1826
SacI GAGCTC 1 cut(s) 1938
SatI GCNGC 7 cut(s) 705, 746, 749, 849, 1818, 1873, 2007
Sau3AI GATC 9 cut(s) 10, 133, 382, 442, 446, 1570, 1766, 1942, 2071
Sau96I GGNCC 6 cut(s) 535, 555, 644, 1623, 1650, 1773
ScaI AGTACT 3 cut(s) 992, 1033, 1991
SchI GAGTC 3 cut(s) 1306, 1352, 1764
ScrFI CCNGG 2 cut(s) 501, 1647
SduI GDGCHC 2 cut(s) 486, 1938
SfaNI GCATC 7 cut(s) 87, 172, 176, 295, 342, 523, 684
SfcI CTRYAG 4 cut(s) 436, 1124, 1527, 2055
SinI GGWCC 3 cut(s) 535, 1623, 1650
SmiMI CAYNNNNRTG 1 cut(s) 1826
SmlI CTYRAG 1 cut(s) 1627
SmoI CTYRAG 1 cut(s) 1627
SpeI ACTAGT 1 cut(s) 1034
SseBI AGGCCT 2 cut(s) 499, 1425
SsiI CCGC 4 cut(s) 8, 692, 954, 1049
SspI AATATT 2 cut(s) 1225, 1502
SspMI CTAG 4 cut(s) 1035, 1421, 1541, 1770
SstI GAGCTC 1 cut(s) 1938
StuI AGGCCT 2 cut(s) 499, 1425
StyD4I CCNGG 2 cut(s) 499, 1645
StyI CCWWGG 2 cut(s) 1420, 1561
TaaI ACNGT 4 cut(s) 255, 980, 1711, 1973
TaiI ACGT 1 cut(s) 2005
TaqI TCGA 7 cut(s) 366, 445, 1300, 1368, 2061, 2070, 2074
TaqII GACCGA 1 cut(s) 1611
TatI WGTACW 6 cut(s) 601, 990, 1031, 1402, 1959, 1989
TscAI CASTG 6 cut(s) 258, 304, 741, 796, 1171, 1828
TseFI GTSAC 1 cut(s) 1490
TseI GCWGC 7 cut(s) 704, 745, 748, 848, 1817, 1872, 2006
Tsp45I GTSAC 1 cut(s) 1490
TspGWI ACGGA 1 cut(s) 1529
TspRI CASTG 6 cut(s) 258, 304, 741, 796, 1171, 1828
VpaK11BI GGWCC 3 cut(s) 535, 1623, 1650
XagI CCTNNNNNAGG 1 cut(s) 313
XapI RAATTY 7 cut(s) 1109, 1274, 1330, 1663, 1902, 1921, 2063
XceI RCATGY 2 cut(s) 1192, 2013
XcmI CCANNNNNNNNNTGG 1 cut(s) 1748
XmaJI CCTAGG 1 cut(s) 1420
XmnI GAANNNNTTC 3 cut(s) 268, 1587, 1784
XspI CTAG 4 cut(s) 1035, 1421, 1541, 1770
ZrmI AGTACT 3 cut(s) 992, 1033, 1991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.