Rh1DG066300

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006658
Physical Location & Seq
Forward (+)
11726969 .. 11742860
15892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG066300.1

Sequence Viewer

Length: 5502 bp
ATGGAGGAAGCCTCTTGTTCACCGGTTTTAGATGGGGAGTTAGTTGGAATTAAATTTGGTTTGGCAACTCATCATGAAATTTGTACAGCATCGATGATGGGATGCTCTATTAGCCATGCCAGTCAGCTTTCCAACCCGTTTCTTGGCCTTCCTCTCGAGTTTGGGAAGTGTGAATCTTGTGGCACTTCCGAACCTGGAAAGTGCGAAGGGCATTTTGGATATATCGACTTACCATTACCGATATATCATCCTAACCACGTCAGTGAGCTGAAGCGCTTGCTGGGTTTTTTGTGCCTGAAGTGCTTAAAAATGAAAAAGAACAAGGTCCCAAGCAAGAGTGCTGGTTTGGCCGAAAGATTGTTATCTGTATGTTGTGAGGATGCTTCACAAGTTTCTATCGCAGAGATTAAACCTACAGATGGGGCTTGCTCCTTGCAATTGAAACTACCTTCTAATAAAAAACCACCGCCTGGTTTTTGGAATTTCCTCGAAAGATATGGTTTTCGTTATGGTGATGGCGTGAAACGCACTTTGCTTCCTAGAGAGGTGATGCAGATTCTCAAAAGAATCCCTGAAGACACCAGAAGAAAACTGGCTGCTAAAGGCTATAATCCTCAAGACGGATACATCTTGAATCATATACCTGTCCCTCCTAATTGCTTGTCTGTGCCTGAAATATCTGATGGTGTTACTGTTATGTCTGCGGATCCTTCAATATCAATGCTCAAAAAAGTTCTAAGGCAAGTTGAAATGATCACTAGTTCAAGGTCTGGTACAGCAAATTTTGAATCTGAAATTGTTGAGGCGAATGAGTTACAAGCAATTGTTGATCAGTATCTTCAAGTTAGGGGTACTGGAAAGGCATCCCGAGATATTGATGCAAGATTTGGAGTGACCAAGGAGCTCAATGATTCTTCAACAAAAGCATGGCTTGAAAAGATGAGGACATTGTTCATCAGAAAGGGTTCAGGTTTCTCTTCCCGCACAGTAATTACTGGGGATGCATATAGAAGGGTGAATGAAGTTGGTATCCCATATGAAATTGCTCAAAGAATAACATTCGAGGAGAAGGTCAATGTACATAATATTAGGCGTCTGCAAGAGCTGGTAGATAGCAAGTTATGCCTAACCTACAGCGATGGTTCATCAACTTACTCTTTAAGGGAAGGTTCAAAGGGGCACACATTTCTGAGGCCTGGTCAAGTGGTGCATAGGAGGATAATGGATGGGGATCTTGTTTTCGTAAATAGGCCTCCAACTACCCATAAACATTCGCTTCAAGCACTTCAAGTTTACGTCCATGATGACCATGTGGTCAAGATTAATCCTCTAATATGTGGACCACTAAGTGCTGACTTTGATGGAGACTGCATTCATTTGTTCTATCCCCAGTCACTTGCGGCAAAGGCAGAAGTTATGGAGCTTTTTTCAGTGGAGAAACAGCTGCTTAGTTCTCACAGTGGCAAGCCCAATTTGCAACTGGCCACCGATTCACTTTTGTCATTAAAAATGATGTTTAAGAAATATTTTTTTGACAAAGCAGCAGCGCAGCAATTGGCCATGTTTGTTTCATCTTCATTGCCACAACCAGCTTTATTGAAAGCTAATTCTGCAGTTCCATATTGGACTGCTTTGCAGATATTGCAGACTGCATTGCCCGCTCATTTTGAGTGCTCTGGAGACAGGCACTTGGTCAGTGCTAGTGAGGTCCTCAAACTTGATTGTAATACAAGTCTTATACCAGCTCTTATCAATGACATTGGTACCTCTATTTTCTTCGAGAAAAGTGCTGAAGATGTTCTCAATTTCTTTAATTCTATGCAGCCATTGCTGATGGAGAATTTATTTTCAGAAGGGTTTAGTGTAGGTTTGGAGGATTTTGCCATGTCTAGGGAGTCCATTCAGGAGATTAGAAAGGGACTTAAAATCATTTCTCCTTTGCTGTATCATATGAGGACAACATATAATGAGCTTGTTGAGTTGCAACTGGAGAATCACATTCGAACGGTGAAACAACCAGTTTCGAATTTTATAACAAATTCATCGGCATTGGGTGATTTAATTGACTCTAAGAGTGATTCAGCCATGAACAAAGTAACTCAACAAGTTGGGTTCCTTGGCCGGCAACTTTATGACCAGGGGAAACTCTACTCAAAAACATTGGTTGAGGATGTGAGCTCTCTGTATCAGAGCAAGTATCCATCTGACATCGTGGATTATCCCTCTGCAGAATTCGGATTAGTTCATAGTGGTTTTGTTCACGGGTTGGATCCTGTTGAGGGAATGATTCATTCCATTGCTACAAGGGAAGTAATTGTTCGGTCCTCAAGAGGGTTGTCTGAACCTGGGACTCTATTCAAAAATCTAATGGCTACTCTCCGAGATGTTGTCATTTGCTATGATGGCACAGTGAGGAATGTGTGCAGTAATTCTATAATTCAGTTCGAGTATGGAGTAAAGAGCGGATCTAGTCCACAAAATTTGTTCCCTGCCGGTGAACCTGTGGGTGTGTTAGCTGCAACAGCAATGTCAAATCCAGCATACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCTTCATGGGATCTGATGAAGGAAATACTGCTCTGCAAAGTCAGTTTCAAGAACGAGCTCATTGATCGTCGTGTAATCTTGTATCTGAATGACTGTGGTTGCGGAAGAAACTACTGCAGAGAACATGCGGCATATTTGGTCAAGAATCAGTTGAAAAAAGTCAGCCTTAAAGATACTGCAGTAGAGTTTATGATTGAATATCAAAATCAGCGAGCTGCGGGAAGCATGGAAATTGACTCTGGCCTTGTAGGTCATGTTCATTTGAATGAGATGCTGTTACGGGAGTTAAATCTGGGAATGTATGAGATCCTTCAAAAATGCGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTTGGGAAGAAGATGAACATCGGTGACATTTTTAAAAGGACAGTTTTATCGTACAGTGACTGTTGTTCTTTTCATCAATCCTCTGCTGAAAATAGATCTGGATCTCCTTGCTTGATGTTCTTCTACCAAGATTTTAATAATTCAGAGTTGGAGGCCATTTCACAAATCTTGGCTGACTTTATTTGCCCAGTTCTTCTAAAAACAGTAATTAAAGGTGATCCTCGAATTTCATCGGCGAACATAATTTGGATCAACTCAGAATCAACAACTTGGATAAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCATGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATGTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAAGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCGACAGCTGTTGCAATGGTGGCGAAAGGTGTTCTCAAAGAGCATCTTATTCTCTTGGCAAACAGTATGACATGCGCTGGAAATTTCGTTGGTTTCAATCCTGGTGGTTATAAAGCACTATCTCGCGCATTGAATATACAAGTACCCTTTACAGAAGCAACATTGTTTACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCTTTGTCGAGCATAGTTGCATCATGTTCCTGGGGTAAACATGTAGCTGTTGGTACTGGCTCCAGATTCGAAATCCTCTGGGACACAAGAGAGGGAGGTTTGAACGAAGAGGGTGGGATAGATGTTTTCAACTTTCTACATATGGTGAATGCTGCAAATGGAGAAGAATCAACTACTGCAGCTTTGGGAGCAGAAATTGATGATCTTGTGCCGGAATATGAAAATGGAGATGTGTCCTTGTCACCAGAACATAACTGTACTTCTGATAAGCCAGTGTTTGAAGATATTGTTGAATTCCCGGATAGTTTTGAAAATGTTCCTGGAAAATCAAGCTGGGATTCATCTCTCAACACCGCTTCTTCTGGTGGGAAAGACTGGGGGGCAGTTAAAGTTGGAAGACAAGATAGCATTTCAGCAGAAACCCAAGTGGATAGCACATCTGCATGGGAGGCCAAGGCTAGTACAGAAGATGCCCCATCTGCATGGGGAACAACCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGAAGCCCCATCTGCATGGGGGACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAACCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGAAGCCCCATCTGCATGGGGGACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAACCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACATCTCCATGGGAGACCAACACTGGTAGAGAAGATTCCACATCGGGCTGGGGGACAAGTACTGCCAGAGAAGATGCCACGACGGCATGGGGGAAAAGCAATGAAAAGAAACATGTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTTGCTGGGGGACAGAAAATCCACAATCTACATGGAGGACAGAAAGCGAGAATGGTTGGTCTGGTGGTGGGGTGAGTAAAGCTGAATCAACAGATGTCCAGTCCAAGAAAGCACAAGAAGATTCTACATCCAATGGTTGGAATGCCTTTTCTGCTGGGCCAACAAAACCCCAAAAGGAGAATGCTGGGTCTGGATGGTGCATGAAAGGTTTTGAGAAGAAAGATGGTAATGAGTTGGAACAAGATAAGTCTACTGCCCGTCATTCTTGGGGGCAGCAAAAGCCCGCCGGTACTTCAGAAGGTGCTTGGGGTCAGGAAAAGCCTGCCGATACTTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCTGATATTTCTGAAGGCGCATGGGGGCAGCAAAAGCCTGATACAACTTCTCAAGGCTCTTGGGGACAGCAAAAGACTCCTGAAATGTCTCAGGGCACTTGGGGAAGGCAAAAGTCTCCTGATATTTCTCAAGGCACATGGGGGCAACAGAAGCCTTCTGATACTTCTCAACCTGCAACTGTGAATCAATGGGATTCTCCAAGTGCAGGAGATGGAAGTGCCAGTGAAAGACAGCAACAATGGGGTCATAATGGAGATGCGAACAAGAAAAGACGTTTTGAAGGTGGCAGGAGTTGGGGTCCAAATGCTGGGGAGTGGAAGAGTAAGAACAGTCGCCCTGCTAATGCTGATTCTAGTGTGGCTGCACTATATACCGCAACGAGACAAAGATTGGATATTTTCACTTCTGAGGAGCAACATGTTCTTTCCCATATTGAGCCGATTATGCTGTCTATTCGAAGAATAATGCATCAATCTGGGTATGTTTTCTATTCATATGCATACTTTTTGTGCTTTCATACCTCTTATCACTTTTCTTGCAAACATAATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1833

Amino Acids

201.62

Weight (kDa)

6.15

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 48 - 281 3.4e-10 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 324 - 478 4.9e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 481 - 626 6e-10 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 755 - 1181 7.1e-10 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2033, 3537
AasI GACNNNNNNGTC 1 cut(s) 1313
Acc36I ACCTGC 1 cut(s) 5128
Acc65I GGTACC 1 cut(s) 1763
AccB1I GGYRCC 1 cut(s) 1763
AccB7I CCANNNNNTGG 3 cut(s) 470, 4722, 5255
AccBSI CCGCTC 2 cut(s) 1661, 2463
AccI GTMKAC 1 cut(s) 4835
AccII CGCG 2 cut(s) 3428, 3552
AcoI YGGCCR 4 cut(s) 348, 1482, 1557, 2119
AcuI CTGAAG 7 cut(s) 290, 317, 594, 1812, 3248, 4863, 4980
AcyI GRCGYC 1 cut(s) 1093
AdeI CACNNNGTG 1 cut(s) 1349
AfeI AGCGCT 1 cut(s) 275
AflIII ACRYGT 3 cut(s) 3682, 4549, 5365
AgeI ACCGGT 1 cut(s) 22
AhlI ACTAGT 3 cut(s) 758, 4223, 4346
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 3221, 3253
AleI CACNNNNGTG 1 cut(s) 261
Alw21I GWGCWC 4 cut(s) 906, 1676, 2180, 2637
Alw26I GTCTC 7 cut(s) 1359, 1674, 4445, 4950, 5040, 5067, 5323
AlwNI CAGNNNCTG 1 cut(s) 2991
Ama87I CYCGRG 2 cut(s) 155, 867
Aor51HI AGCGCT 1 cut(s) 275
AseI ATTAAT 1 cut(s) 1323
AsiGI ACCGGT 1 cut(s) 22
Asp700I GAANNNNTTC 3 cut(s) 964, 1797, 3957
Asp718I GGTACC 1 cut(s) 1763
AspLEI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 4967
AsuC2I CCSGG 1 cut(s) 3941
AsuII TTCGAA 4 cut(s) 2002, 2024, 3711, 5404
AvaI CYCGRG 2 cut(s) 155, 867
AvaII GGWCC 9 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4299, 4338, 5246
BaeGI GKGCMC 2 cut(s) 1182, 5045
BaeI ACNNNNGTAYC 4 cut(s) 1747, 1780, 2742, 2775
BalI TGGCCA 2 cut(s) 1484, 1559
BamHI GGATCC 2 cut(s) 706, 2269
BanI GGYRCC 1 cut(s) 1763
BanII GRGCYC 3 cut(s) 906, 2180, 2637
BbsI GAAGAC 2 cut(s) 582, 4045
Bbv12I GWGCWC 4 cut(s) 906, 1676, 2180, 2637
BceAI ACGGC 2 cut(s) 4536, 4952
BcgI CGANNNNNNTGC 2 cut(s) 1769, 1803
BciT130I CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BciVI GTATCC 3 cut(s) 617, 1040, 2208
BclI TGATCA 3 cut(s) 753, 829, 3406
BcnI CCSGG 1 cut(s) 3941
BcoDI GTCTC 7 cut(s) 1359, 1674, 4445, 4950, 5040, 5067, 5323
BcuI ACTAGT 3 cut(s) 758, 4223, 4346
BfmI CTRYAG 7 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819
BfoI RGCGCY 2 cut(s) 277, 3424
BfuAI ACCTGC 1 cut(s) 5128
BfuI GTATCC 3 cut(s) 617, 1040, 2208
BglI GCCNNNNNGGC 1 cut(s) 4520
BglII AGATCT 1 cut(s) 3026
BmcAI AGTACT 1 cut(s) 4498
Bme18I GGWCC 9 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4299, 4338, 5246
BmeT110I CYCGRG 2 cut(s) 155, 867
BmgBI CACGTC 1 cut(s) 259
BmrI ACTGGG 4 cut(s) 1005, 1384, 3113, 4027
BmuI ACTGGG 4 cut(s) 1005, 1384, 3113, 4027
BpiI GAAGAC 2 cut(s) 582, 4045
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 4 cut(s) 1698, 2009, 3278, 3688
Bpu14I TTCGAA 4 cut(s) 2002, 2024, 3711, 5404
BpuEI CTTGAG 4 cut(s) 600, 2311, 4983, 5061
BpuMI CCSGG 1 cut(s) 3941
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 5 cut(s) 361, 834, 1230, 2948, 3031
BsaHI GRCGYC 1 cut(s) 1093
BsaI GGTCTC 1 cut(s) 4445
BsaJI CCNNGG 8 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4445, 4563
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 2 cut(s) 5230, 5260
Bse118I RCCGGY 4 cut(s) 22, 2121, 2492, 4871
Bse3DI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4543
Bse8I GATNNNNATC 5 cut(s) 361, 834, 1230, 2948, 3031
BseBI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 8 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4445, 4563
BseJI GATNNNNATC 5 cut(s) 361, 834, 1230, 2948, 3031
BseMI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4543
BseMII CTCAG 5 cut(s) 1181, 3201, 4934, 5051, 5346
BseRI GAGGAG 2 cut(s) 1079, 5372
BseSI GKGCMC 2 cut(s) 1182, 5045
BseYI CCCAGC 7 cut(s) 280, 3975, 4485, 4590, 4739, 4769, 5255
BsgI GTGCAG 4 cut(s) 2443, 3395, 5172, 5295
Bsh1236I CGCG 2 cut(s) 3428, 3552
BshNI GGYRCC 1 cut(s) 1763
BshTI ACCGGT 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 4 cut(s) 906, 1676, 2180, 2637
BsiHKCI CYCGRG 2 cut(s) 155, 867
BsiSI CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4872
BsmAI GTCTC 7 cut(s) 1359, 1674, 4445, 4950, 5040, 5067, 5323
BsmI GAATGC 4 cut(s) 1371, 3796, 4732, 4771
Bso31I GGTCTC 1 cut(s) 4445
BsoBI CYCGRG 2 cut(s) 155, 867
Bsp119I TTCGAA 4 cut(s) 2002, 2024, 3711, 5404
Bsp1286I GDGCHC 6 cut(s) 906, 1182, 1676, 2180, 2637, 5045
Bsp1407I TGTACA 2 cut(s) 83, 1078
Bsp19I CCATGG 1 cut(s) 4445
Bsp68I TCGCGA 1 cut(s) 3428
BspCNI CTCAG 5 cut(s) 1182, 3200, 4933, 5050, 5347
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 2 cut(s) 3428, 3552
BspHI TCATGA 1 cut(s) 73
BspMAI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
BspMI ACCTGC 1 cut(s) 5128
BspT104I TTCGAA 4 cut(s) 2002, 2024, 3711, 5404
BspT107I GGYRCC 1 cut(s) 1763
BspTNI GGTCTC 1 cut(s) 4445
BsrBI CCGCTC 2 cut(s) 1661, 2463
BsrDI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4543
BsrFI RCCGGY 4 cut(s) 22, 2121, 2492, 4871
BsrGI TGTACA 2 cut(s) 83, 1078
BssAI RCCGGY 4 cut(s) 22, 2121, 2492, 4871
BssECI CCNNGG 8 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4445, 4563
BssNI GRCGYC 1 cut(s) 1093
BssT1I CCWWGG 5 cut(s) 897, 2116, 4095, 4445, 4563
Bst2UI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
Bst6I CTCTTC 4 cut(s) 982, 2584, 3744, 5261
BstACI GRCGYC 1 cut(s) 1093
BstAPI GCANNNNNTGC 3 cut(s) 1122, 1642, 1828
BstAUI TGTACA 2 cut(s) 83, 1078
BstBI TTCGAA 4 cut(s) 2002, 2024, 3711, 5404
BstC8I GCNNGC 8 cut(s) 278, 427, 1466, 1659, 2123, 2790, 4869, 4908
BstDEI CTNAG 9 cut(s) 737, 1190, 1346, 1448, 2070, 3187, 4920, 5037, 5355
BstDSI CCRYGG 1 cut(s) 4445
BstFNI CGCG 2 cut(s) 3428, 3552
BstH2I RGCGCY 2 cut(s) 277, 3424
BstHHI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 4967
BstMAI GTCTC 7 cut(s) 1359, 1674, 4445, 4950, 5040, 5067, 5323
BstNI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BstNSI RCATGY 5 cut(s) 2705, 3501, 3686, 4553, 5369
BstSFI CTRYAG 7 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819
BstSLI GKGCMC 2 cut(s) 1182, 5045
BstUI CGCG 2 cut(s) 3428, 3552
BstV2I GAAGAC 2 cut(s) 582, 4045
BstX2I RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
BstXI CCANNNNNNTGG 8 cut(s) 4125, 4170, 4209, 4248, 4293, 4332, 4371, 4416
BstYI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
Bsu15I ATCGAT 1 cut(s) 92
BsuI GTATCC 3 cut(s) 617, 1040, 2208
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 4445
BtgZI GCGATG 1 cut(s) 1152
BtrI CACGTC 1 cut(s) 259
BtuMI TCGCGA 1 cut(s) 3428
BveI ACCTGC 1 cut(s) 5128
Cac8I GCNNGC 8 cut(s) 278, 427, 1466, 1659, 2123, 2790, 4869, 4908
CaiI CAGNNNCTG 1 cut(s) 2991
CciI TCATGA 1 cut(s) 73
CfoI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 4967
Cfr10I RCCGGY 4 cut(s) 22, 2121, 2492, 4871
ClaI ATCGAT 1 cut(s) 92
CseI GACGC 1 cut(s) 1082
CspAI ACCGGT 1 cut(s) 22
CspCI CAANNNNNGTGG 4 cut(s) 2463, 2498, 3511, 3546
DdeI CTNAG 9 cut(s) 737, 1190, 1346, 1448, 2070, 3187, 4920, 5037, 5355
DraI TTTAAA 1 cut(s) 2965
DraIII CACNNNGTG 1 cut(s) 1349
DrdI GACNNNNNNGTC 1 cut(s) 1313
DseDI GACNNNNNNGTC 1 cut(s) 1313
EaeI YGGCCR 4 cut(s) 348, 1482, 1557, 2119
Eam1104I CTCTTC 4 cut(s) 982, 2584, 3744, 5261
EarI CTCTTC 4 cut(s) 982, 2584, 3744, 5261
Ecl136II GAGCTC 3 cut(s) 904, 2178, 2635
Eco130I CCWWGG 5 cut(s) 897, 2116, 4095, 4445, 4563
Eco147I AGGCCT 2 cut(s) 1195, 1252
Eco24I GRGCYC 3 cut(s) 906, 2180, 2637
Eco31I GGTCTC 1 cut(s) 4445
Eco47I GGWCC 9 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4299, 4338, 5246
Eco47III AGCGCT 1 cut(s) 275
Eco53kI GAGCTC 3 cut(s) 904, 2178, 2635
Eco57I CTGAAG 7 cut(s) 290, 317, 594, 1812, 3248, 4863, 4980
Eco88I CYCGRG 2 cut(s) 155, 867
EcoICRI GAGCTC 3 cut(s) 904, 2178, 2635
EcoO109I RGGNCCY 2 cut(s) 325, 1708
EcoRI GAATTC 2 cut(s) 2231, 3935
EcoRII CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
EcoT14I CCWWGG 5 cut(s) 897, 2116, 4095, 4445, 4563
EcoT22I ATGCAT 4 cut(s) 1006, 3292, 5418, 5449
EcoT38I GRGCYC 3 cut(s) 906, 2180, 2637
ErhI CCWWGG 5 cut(s) 897, 2116, 4095, 4445, 4563
FalI AAGNNNNNCTT 6 cut(s) 915, 947, 2727, 2759, 3456, 3488
FauI CCCGC 4 cut(s) 989, 1666, 2788, 4876
FauNDI CATATG 6 cut(s) 1036, 1950, 3358, 3636, 3783, 5443
FbaI TGATCA 3 cut(s) 753, 829, 3406
FblI GTMKAC 1 cut(s) 4835
FriOI GRGCYC 3 cut(s) 906, 2180, 2637
GlaI GCGC 6 cut(s) 275, 1548, 3422, 3502, 3553, 4966
GsaI CCCAGC 7 cut(s) 284, 3979, 4489, 4594, 4743, 4773, 5259
GsuI CTGGAG 4 cut(s) 1698, 2009, 3278, 3688
HaeII RGCGCY 2 cut(s) 277, 3424
HapII CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4872
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 4967
Hin1I GRCGYC 1 cut(s) 1093
Hin6I GCGC 6 cut(s) 274, 1547, 3421, 3501, 3552, 4965
HinP1I GCGC 6 cut(s) 274, 1547, 3421, 3501, 3552, 4965
HpaII CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4872
Hpy166II GTNNAC 9 cut(s) 20, 1294, 1340, 2260, 2474, 2498, 3594, 3680, 4836
Hpy8I GTNNAC 9 cut(s) 20, 1294, 1340, 2260, 2474, 2498, 3594, 3680, 4836
Hpy99I CGWCG 2 cut(s) 2649, 4522
HpyCH4IV ACGT 3 cut(s) 258, 1296, 5221
HpyF3I CTNAG 9 cut(s) 737, 1190, 1346, 1448, 2070, 3187, 4920, 5037, 5355
HpySE526I ACGT 3 cut(s) 258, 1296, 5221
Hsp92I GRCGYC 1 cut(s) 1093
HspAI GCGC 6 cut(s) 274, 1547, 3421, 3501, 3552, 4965
KpnI GGTACC 1 cut(s) 1767
KroI GCCGGC 1 cut(s) 2121
KroNI GCCGGC 1 cut(s) 2123
Ksp22I TGATCA 3 cut(s) 753, 829, 3406
LmnI GCTCC 6 cut(s) 434, 901, 1420, 3707, 3830, 5359
MaeII ACGT 3 cut(s) 258, 1296, 5221
MaeIII GTNAC 9 cut(s) 688, 813, 892, 1392, 2095, 2853, 2954, 2987, 3882
MbiI CCGCTC 2 cut(s) 1661, 2463
MfeI CAATTG 3 cut(s) 437, 822, 1553
MflI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
MhlI GDGCHC 6 cut(s) 906, 1182, 1676, 2180, 2637, 5045
MlsI TGGCCA 2 cut(s) 1484, 1559
MluNI TGGCCA 2 cut(s) 1484, 1559
MlyI GAGTC 7 cut(s) 1904, 2060, 2344, 2807, 4177, 4300, 5017
MmeI TCCRAC 8 cut(s) 25, 156, 1280, 2247, 3060, 4015, 4703, 4800
Mox20I TGGCCA 2 cut(s) 1484, 1559
Mph1103I ATGCAT 4 cut(s) 1006, 3292, 5418, 5449
MroNI GCCGGC 1 cut(s) 2121
MroXI GAANNNNTTC 3 cut(s) 964, 1797, 3957
MscI TGGCCA 2 cut(s) 1484, 1559
Msp20I TGGCCA 2 cut(s) 1484, 1559
MspA1I CMGCKG 3 cut(s) 1444, 3434, 3623
MspI CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4872
MunI CAATTG 3 cut(s) 437, 822, 1553
Mva1269I GAATGC 4 cut(s) 1371, 3796, 4732, 4771
MvaI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
MvnI CGCG 2 cut(s) 3428, 3552
NaeI GCCGGC 1 cut(s) 2123
NciI CCSGG 1 cut(s) 3941
NcoI CCATGG 1 cut(s) 4445
NdeI CATATG 6 cut(s) 1036, 1950, 3358, 3636, 3783, 5443
NgoMIV GCCGGC 1 cut(s) 2121
NmuCI GTSAC 5 cut(s) 892, 1392, 2954, 2987, 3882
NruI TCGCGA 1 cut(s) 3428
NsiI ATGCAT 4 cut(s) 1006, 3292, 5418, 5449
NspI RCATGY 5 cut(s) 2705, 3501, 3686, 4553, 5369
NspV TTCGAA 4 cut(s) 2002, 2024, 3711, 5404
OliI CACNNNNGTG 1 cut(s) 261
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 1 cut(s) 73
PceI AGGCCT 2 cut(s) 1195, 1252
PciI ACATGT 3 cut(s) 3682, 4549, 5365
PctI GAATGC 4 cut(s) 1371, 3796, 4732, 4771
PdiI GCCGGC 1 cut(s) 2123
PdmI GAANNNNTTC 3 cut(s) 964, 1797, 3957
PflMI CCANNNNNTGG 3 cut(s) 470, 4722, 5255
PfoI TCCNGGA 2 cut(s) 3939, 3961
PinAI ACCGGT 1 cut(s) 22
PleI GAGTC 7 cut(s) 1903, 2060, 2344, 2807, 4177, 4300, 5017
PpsI GAGTC 7 cut(s) 1903, 2060, 2344, 2807, 4177, 4300, 5017
PpuMI RGGWCCY 2 cut(s) 325, 1708
PscI ACATGT 3 cut(s) 3682, 4549, 5365
PshBI ATTAAT 1 cut(s) 1323
PsiI TTATAA 2 cut(s) 2033, 3537
Psp124BI GAGCTC 3 cut(s) 906, 2180, 2637
Psp5II RGGWCCY 2 cut(s) 325, 1708
Psp6I CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
PspFI CCCAGC 7 cut(s) 280, 3975, 4485, 4590, 4739, 4769, 5255
PspGI CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
PspPPI RGGWCCY 2 cut(s) 325, 1708
PsrI GAACNNNNNNTAC 2 cut(s) 2817, 2849
PstI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
PstNI CAGNNNCTG 1 cut(s) 2991
PsuI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
PvuII CAGCTG 3 cut(s) 1444, 3434, 3623
RruI TCGCGA 1 cut(s) 3428
SacI GAGCTC 3 cut(s) 906, 2180, 2637
ScaI AGTACT 1 cut(s) 4498
SchI GAGTC 7 cut(s) 1904, 2060, 2344, 2807, 4177, 4300, 5017
SduI GDGCHC 6 cut(s) 906, 1182, 1676, 2180, 2637, 5045
SfcI CTRYAG 7 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819
Sfr274I CTCGAG 1 cut(s) 155
SfuI TTCGAA 4 cut(s) 2002, 2024, 3711, 5404
SinI GGWCC 9 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4299, 4338, 5246
SlaI CTCGAG 1 cut(s) 155
SmlI CTYRAG 5 cut(s) 155, 615, 2326, 4998, 5076
SmoI CTYRAG 5 cut(s) 155, 615, 2326, 4998, 5076
SpeI ACTAGT 3 cut(s) 758, 4223, 4346
SseBI AGGCCT 2 cut(s) 1195, 1252
SspI AATATT 2 cut(s) 1087, 1526
SstI GAGCTC 3 cut(s) 906, 2180, 2637
StuI AGGCCT 2 cut(s) 1195, 1252
StyI CCWWGG 5 cut(s) 897, 2116, 4095, 4445, 4563
TaiI ACGT 3 cut(s) 261, 1299, 5224
TaqII GACCGA 1 cut(s) 2310
TatI WGTACW 7 cut(s) 83, 1078, 3899, 4103, 4226, 4349, 4496
TauI GCSGC 2 cut(s) 1403, 2708
TseFI GTSAC 5 cut(s) 892, 1392, 2954, 2987, 3882
Tsp45I GTSAC 5 cut(s) 892, 1392, 2954, 2987, 3882
TspGWI ACGGA 1 cut(s) 636
Van91I CCANNNNNTGG 3 cut(s) 470, 4722, 5255
VpaK11BI GGWCC 9 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4299, 4338, 5246
VspI ATTAAT 1 cut(s) 1323
XceI RCATGY 5 cut(s) 2705, 3501, 3686, 4553, 5369
XcmI CCANNNNNNNNNTGG 3 cut(s) 589, 1477, 4614
XhoI CTCGAG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 4835
XmnI GAANNNNTTC 3 cut(s) 964, 1797, 3957
ZrmI AGTACT 1 cut(s) 4498
Zsp2I ATGCAT 4 cut(s) 1006, 3292, 5418, 5449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.