Rw1G005130

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006658
Physical Location & Seq
Forward (+)
9896338 .. 9911498
15161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005130.1

Sequence Viewer

Length: 5826 bp
ATGGAGGAAGCGTCTTGTTCACCGGTTTTAGATGGGGAGTTAGTTGGAATTAAATTTGGTTTGGCAACTCATCATGAAATTTGTACAGCATCGATGATGGGATGCTCTATTAGCCATGCCAGTCAGCTTTCCAACCCGTTTCTTGGCCTTCCTCTCGAGTTTGGGAAGTGTGAATCTTGTGGCACTTCCGAACCTGGAAAGTGCGAAGGGCATTTTGGATATATCGACTTACCATTACCGATATATCATCCTAACCACGTCAGTGAGCTGAAGCGCTTGCTGAGTTTTTTGTGCCTGAAGTGCTTAAAAATGAAAAAGAACAAGGTCCCAAGCAAGAGTGCTGGTTTGGCCGAAAGATTGTTATCTGTATGTTGTGAGGATGCTTCACAAGTTTCTATCGCAGAGATTAAACCTACAGATGGGGCTTGCTCCTTGCAATTGAAACTACCTTCTAATAAAAAACCACCGCCTGGTTTTTGGAATTTCCTCGAAAGATATGGTTTTCGTTATGGTGATGGCGTGAAACGCACTTTGCTTCCTAGAGAGGTGATGCAGATTCTCAAAAGAATCCCTGAAGACACCAGAAGAAAACTGGCTGCTAAAGGCTATAATCCTCAAGACGGATACATCTTGAATCATATACCTGTCCCTCCTAATTGCTTGTCTGTGCCTGAAATATCTGATGGTGTTACTGTTATGTCTGCGGATCCTTCAATATCAATGCTCAAAAAAGTTCTAAGGCAAGTTGAAATGATCACTAGTTCAAGGTCTGGTACAGCAAATTTTGAATCTGAAATTGTTGAGGCGAATGAGTTACAAGCAATTGTTGATCAGTATCTTCAAGTTAGGGGTACTGGAAAGGCATCCCGAGATATTGATGCAAGATTTGGAGTGACCAAGGAGCTCAATGATTCTTCAACAAAAGCATGGCTTGAAAAGATGAGGACGTTGTTCATCAGAAAGGGTTCAGGTTTCTCTTCCCGCACAGTAATTACTGGGGATGCATATAGAAGGGTGAATGAAGTTGGTATCCCATATGAAATTGCTCAAAGAATAACATTTGAGGAGAAGGTCAATGTACATAATATTAGGCGTTTGCAAGAGCTGGTAGATAGCAAGTTATGCCTAACCTACAGCGATGGTTCATCAACTTACTCTTTAAGGGAAGGTTCAAAGGGGCACACATTTCTGAGGCCTGGTCAAGTGGTGCATAGGAGGATAATGGATGGGGATCTTGTTTTCGTAAATAGGCCTCCAACTACCCATAAACATTCGCTTCAAGCACTTCAAGTTTACGTCCATGATGACCATGTGGTCAAGATTAATCCTCTAATATGTGGACCACTAAGTGCTGACTTTGATGGAGACTGCATTCATTTGTTCTATCCCCAGTCACTTGCGGCAAAGGCAGAAGTTATGGAGCTTTTTTCAGTGGAGAAACAGCTGCTTAGTTCTCACAGTGGCAAGCCCAATCTGCAACTGGCCACCGATTCACTTTTGTCATTAAAAATGATGTTTAAGAAATATTTTTTCGACAAAGCAGCAGCGCAACAATTGGCCATGTTTGTTTCATCTTCATTGCCACAACCAGCTTTATTGAAAGCTAATTCTGCAGTTCCATATTGGACTGCTTTGCAGATATTGCAGACTGCATTGCCCGCTCATTTTGAGTGCTCTGGAGACAGGCACTTGGTCAGTGCTAGTGAGGTCCTCAAACTTGATTGTAATACAAGTCTTATACCAGCTCTTATCAATGACATTGGTACCTCTATTTTCTTCGAGAAAAGTGCTGAAGATGTTCTCAATTTCTTTAATTCTATGCAGCCATTGCTGATGGAGAATTTATTTTCAGAAGGGTTTAGTGTAGGTTTGGAGGATTTTGCCATGTCTAGGGAGTCCATTCAGGAGATTAGAAAGGGACTTAAAATCATTTCTCCTTTGCTGTATCATATGAGGACAACATATAATGAGCTTGTTGAGTTGCAACTGGAGAATCACATTCGAACGGTGAAACAACCAGTTTCGAATTTTATAACAAATTCATCGGCATTGGGTGATTTAATTGACTCTAAGAGTGATTCAGCCATGAACAAAGTAACTCAACAAGTTGGGTTCCTTGGCCGGCAACTTTATGACCAGGGGAAACTCTACTCAAAAACATTGGTTGAGGATGTGAGCTCTCTGTATCAGAGCAAGTATCCATCTGACATTGTGGATTATCCCTCTGCAGAATTCGGATTAGTTCATAGTGGTTTTGTTCACGGGTTGGATCCTGTTGAGGGAATGATTCATTCCATTGCTACAAGGGAAGTAATTGTTCGGTCCTCAAGAGGGTTGTCTGAACCTGGGACTCTATTCAAAAATCTAATGGCTACTCTCCGAGATGTTGTCATTTGCTATGATGGCACAGTGAGGAATGTGTGCAGTAATTCTATAATTCAGTTTGAGTATGGAGTAAAGAGCGGATCTAGTCCACAAAATTTGTTCCCTGCCGGTGAACCTGTGGGTGTGTTAGCTGCAACAGCAATGTCAAATCCAGCATACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCTTCATGGGATCTGATGAAGGAAATACTGCTCTGCAAAGTTAGTTTCAAGAACGAGCTCATTGATCGTCGTGTAATCTTGTATCTGAATGACTGTGGTTGCGGAAGAAACTACTGCAGAGAACATGCGGCATATTTGGTCAAGAATCAGTTGAAAAAAGTCAGCCTTAAAGATACTGCAGTAGAGTTTATGATTGAATATCAAAATCAGCGAGCTGCGGGAAGCATGGAAATTGACTCTGGCCTTGTAGGTCATGTTCATTTGAATGAGATGCTGTTACGGGAGTTAAATCTGGGAATGTATGAGATCCTTCAAAAATGCGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTTGGGAAGAAGATGAACATTGGTGACATTTTTAAAAGGACAGTTTTATCGTACAGTGACTGTTGTTCTTTTCATCAATCCTCTGCTGACAATAGATCTGGATCTCCTTGCTTGATGTTCTTCTACCAAGATTTTAATAATTCAGAGTTGGAGGCCATTTCACAAATCTTGGCTGACTTTATTTGCCCAGTTCTTCTAAAAACAGTAATTAAAGGTGATCCTCGAATTTCGTCGGCGAACATAATTTGGATAAACTCAGAATCAACAACTTGGATAAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCATGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATGTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAAGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTTGCGACAGCTGTTGCAATGGTGGCTAAAGGTGTTCTCAAAGAGCATCTTATTCTCTTGGCAAACAGTATGACATGCGCTGGAAATTTCGTTGGTTTCAATCCTGGTGGTTATAAAGCACTATCTCGCGCATTGAATATACAAGTACCGTTTACAGAAGCAACATTGTTTACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCTTTGTCGAGCATAGTTGCATCATGTTCCTGGGGAAAACATGTAGCTGTTGGTACTGGCTCCAGATTCGAAATCCTCTGGGACACAAGAGAGGGAGGTTTGAACGAAGAGGGTGGGATAGATGTTTTCAACTTTCTACATATGGTGAATGCTGCAAATGGAGAAGAATCAACTACTGCAGCTTTGGGAGCAGAAATTGATGATCTTGTGCCGGAATATGAAAATGGAGATGTGTCCTTGTCACCAGAACATAACTGTACTTCTGATAAGCCAGTGTTTGAAGATATTGTTGAATTCCTGGATAGTTTCGAAAATGTTCCTGGAAAATCAAGCTGGGATTCATCTCTCAACACCGCATCTTCTGGTGGGAAAGACTGGGGGGCAGTTAGAGTTGGAAGACAAGATTGCATTTCAGCAGAAACCCAAGTGGATAGCACATCTGCATGGGGGGCCAAGGCTAGTACGGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGATGCATGGGGGGCCAAGGCTAGTACAGAAGATACCCCATCTGCATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGCACAGAAGATGCATGGGGGGCCAAGGCTAGTACAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCTCATCTGCGTGGGGGACCAAGACTCATACAGAAGATGCCCCATCTGCATGGGGGAGCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTGCATCTCCATGGGAGACCAACACTGGTAGAGAAGATTCCACATCGGGCTGGGGGACAAGTACTGCCAGAGAAGATGCCACGACGGCATGGGGGAAAAGCAATGAAAAGAAACATGTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTTGCTGGGGGACAGAAAATCGACAATCTACATGGAGGACAGAAGGCGAGAATGGTTGGTCTGGTGGTGGGGTGAGTAAAGCTGAATCAACAGATGTCCAGTCCAAGAAAGCACAAGAAGATTCTACATCCAATGGTTGGAATGCCTTTTCTGCTGGGCCAACAAAACCCCAAAAGGAGAATGCTGGGTCTGGATGGTGCATGAAAGGTTTTGAGAAGAAAGATGGTAATGAGTTGGAACAAGATAAGTCTACTGCCCGTCATTCTTGGGGGCAGCAAAAGCCCGCCGGTACTTCAGAAGGTGCTTGGGGTCAGGAAGAGCCTGCCGATACTTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCTGATATTTCTGAAGGCGCATGGGGGCAGCAAAAGCCTGATACAACTTCTCACGGCTCTTGGGGACAGCAAAAGACTCCTGAAATGTCTCAGGGCACTTGGGGAAGGCAAAAGTCTCCTGATATTTCTCAAGGCACATGGGGGCAACAGAAGCCTTCTGATACTTCTCAACTTGCAACTGTGAATCAATGGGATTCTCCAAGTGCAGGAGATGGAAGTGCCGGTGAAAGACAGCAACCATGGGGTCATAATGGAGATGCGAACAAGAAAAGACGTTTTGAAGGTGGCAGGAGTTGGGGTCCAAATGCTGGGGAGTGGAAGAGTAAGAACAGTCGCCCTGCTAATGCTGATTCTAGTGTGGCTGCACTATATACCGCAACGAGACAAAGATTGGATATTTTCACTTCTGAGGAGCAACTTGTTCTTTCCCATATTGAGCCGATTATGCTGTCTATTCGAAGAATAATGCATCAATCTGGCTACAACGATGGAGATCGGTTGTCAGATGAGGATCAATCATACATACTTGAAAATGTGTTCACACACCACCCAGATAAAGATGTTAAGATGGGTTCTGGAATTGACTATTTTACAGTTGATAGGCATGGAAGTTTTCAAGAAAGCCGATGCCTTTTCGTCATATCAACTGATGGTCGCAAGGAGGACTTTTCCTACAGAAAATGCCTGCAAAATATGATAAAGGGAAAGTATCCTGAGCTAGCTGAAGCATTCAATGACAAGTACTTCAGCAAACCTCGGCGGAATCCCATGTTCGGTCAGACCCCTCCAGAGAGAACTGGGAATGAGGAGACACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1941

Amino Acids

213.63

Weight (kDa)

5.96

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 48 - 281 1.6e-10 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 324 - 478 5.2e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 481 - 626 6.5e-10 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 755 - 1181 7.8e-10 RNA polymerase Rpb1, domain 5
DUF3223 PF11523 1823 - 1898 3.3e-27 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2033, 3537
AasI GACNNNNNNGTC 1 cut(s) 1313
Acc65I GGTACC 1 cut(s) 1763
AccB1I GGYRCC 1 cut(s) 1763
AccB7I CCANNNNNTGG 3 cut(s) 470, 4782, 5315
AccBSI CCGCTC 2 cut(s) 1661, 2463
AccI GTMKAC 1 cut(s) 4895
AccII CGCG 1 cut(s) 3552
AcoI YGGCCR 4 cut(s) 348, 1482, 1557, 2119
AcuI CTGAAG 9 cut(s) 290, 317, 594, 1812, 3248, 4923, 5040, 5737, 5751
AdeI CACNNNGTG 1 cut(s) 1349
AfeI AGCGCT 1 cut(s) 275
AflIII ACRYGT 2 cut(s) 3682, 4609
AgeI ACCGGT 1 cut(s) 22
AhlI ACTAGT 2 cut(s) 758, 4451
AjiI CACGTC 1 cut(s) 259
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 3221, 3253
AleI CACNNNNGTG 1 cut(s) 261
Alw21I GWGCWC 4 cut(s) 906, 1676, 2180, 2637
Alw26I GTCTC 8 cut(s) 1359, 1674, 4505, 5010, 5100, 5127, 5383, 5810
AlwNI CAGNNNCTG 1 cut(s) 2991
Ama87I CYCGRG 2 cut(s) 155, 867
Aor51HI AGCGCT 1 cut(s) 275
AseI ATTAAT 1 cut(s) 1323
AsiGI ACCGGT 1 cut(s) 22
Asp700I GAANNNNTTC 3 cut(s) 964, 1797, 3957
Asp718I GGTACC 1 cut(s) 1763
AspLEI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 5027
AsuII TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5464
AsuNHI GCTAGC 1 cut(s) 5725
AvaI CYCGRG 2 cut(s) 155, 867
AvaII GGWCC 8 cut(s) 325, 1340, 1708, 2322, 4176, 4290, 4404, 5306
BaeGI GKGCMC 2 cut(s) 1182, 5105
BaeI ACNNNNGTAYC 4 cut(s) 1747, 1780, 2742, 2775
BalI TGGCCA 2 cut(s) 1484, 1559
BamHI GGATCC 2 cut(s) 706, 2269
BanI GGYRCC 1 cut(s) 1763
BanII GRGCYC 3 cut(s) 906, 2180, 2637
BbsI GAAGAC 2 cut(s) 582, 4045
Bbv12I GWGCWC 4 cut(s) 906, 1676, 2180, 2637
BceAI ACGGC 3 cut(s) 4596, 5012, 5077
BcgI CGANNNNNNTGC 2 cut(s) 1769, 1803
BciVI GTATCC 4 cut(s) 617, 1040, 2208, 5727
BclI TGATCA 3 cut(s) 753, 829, 3406
BcoDI GTCTC 8 cut(s) 1359, 1674, 4505, 5010, 5100, 5127, 5383, 5810
BcuI ACTAGT 2 cut(s) 758, 4451
BfmI CTRYAG 8 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819, 5680
BfoI RGCGCY 2 cut(s) 277, 3424
BfuI GTATCC 4 cut(s) 617, 1040, 2208, 5727
BglI GCCNNNNNGGC 1 cut(s) 4580
BglII AGATCT 1 cut(s) 3026
BmcAI AGTACT 2 cut(s) 4558, 5750
Bme18I GGWCC 8 cut(s) 325, 1340, 1708, 2322, 4176, 4290, 4404, 5306
BmeT110I CYCGRG 2 cut(s) 155, 867
BmgBI CACGTC 1 cut(s) 259
BmrI ACTGGG 5 cut(s) 1005, 1384, 3113, 4027, 5814
BmtI GCTAGC 1 cut(s) 5729
BmuI ACTGGG 5 cut(s) 1005, 1384, 3113, 4027, 5814
BpiI GAAGAC 2 cut(s) 582, 4045
BpmI CTGGAG 5 cut(s) 1698, 2009, 3278, 3688, 5778
Bpu10I CCTNAGC 1 cut(s) 5721
Bpu14I TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5464
BpuEI CTTGAG 3 cut(s) 600, 2311, 5121
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 6 cut(s) 361, 834, 1230, 3031, 5499, 5517
BsaI GGTCTC 1 cut(s) 4505
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 4 cut(s) 26, 5290, 5320, 5807
Bse118I RCCGGY 5 cut(s) 22, 2121, 2492, 4931, 5228
Bse3DI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4603
Bse8I GATNNNNATC 6 cut(s) 361, 834, 1230, 3031, 5499, 5517
BseCI ATCGAT 1 cut(s) 92
BseJI GATNNNNATC 6 cut(s) 361, 834, 1230, 3031, 5499, 5517
BseMI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4603
BseMII CTCAG 7 cut(s) 272, 1181, 3201, 4994, 5111, 5406, 5712
BseRI GAGGAG 2 cut(s) 1079, 5432
BseSI GKGCMC 2 cut(s) 1182, 5105
BseYI CCCAGC 6 cut(s) 3975, 4545, 4650, 4799, 4829, 5315
BsgI GTGCAG 4 cut(s) 2443, 3395, 5232, 5355
Bsh1236I CGCG 1 cut(s) 3552
BshNI GGYRCC 1 cut(s) 1763
BshTI ACCGGT 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 4 cut(s) 906, 1676, 2180, 2637
BsiHKCI CYCGRG 2 cut(s) 155, 867
BsiSI CCGG 6 cut(s) 23, 2122, 2493, 3854, 4932, 5229
BsmAI GTCTC 8 cut(s) 1359, 1674, 4505, 5010, 5100, 5127, 5383, 5810
BsmI GAATGC 5 cut(s) 1371, 3796, 4792, 4831, 5735
Bso31I GGTCTC 1 cut(s) 4505
BsoBI CYCGRG 2 cut(s) 155, 867
Bsp119I TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5464
Bsp1286I GDGCHC 6 cut(s) 906, 1182, 1676, 2180, 2637, 5105
Bsp1407I TGTACA 2 cut(s) 83, 1078
Bsp19I CCATGG 2 cut(s) 4505, 5246
BspCNI CTCAG 7 cut(s) 273, 1182, 3200, 4993, 5110, 5407, 5713
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 1 cut(s) 3552
BspHI TCATGA 1 cut(s) 73
BspMAI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
BspOI GCTAGC 1 cut(s) 5729
BspQI GCTCTTC 1 cut(s) 4956
BspT104I TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5464
BspT107I GGYRCC 1 cut(s) 1763
BspTNI GGTCTC 1 cut(s) 4505
BsrBI CCGCTC 2 cut(s) 1661, 2463
BsrDI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4603
BsrFI RCCGGY 5 cut(s) 22, 2121, 2492, 4931, 5228
BsrGI TGTACA 2 cut(s) 83, 1078
BssAI RCCGGY 5 cut(s) 22, 2121, 2492, 4931, 5228
BssT1I CCWWGG 8 cut(s) 897, 2116, 4095, 4209, 4323, 4505, 4623, 5246
Bst6I CTCTTC 5 cut(s) 982, 2584, 3744, 4956, 5321
BstAPI GCANNNNNTGC 3 cut(s) 1122, 1642, 1828
BstAUI TGTACA 2 cut(s) 83, 1078
BstBI TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5464
BstDSI CCRYGG 2 cut(s) 4505, 5246
BstFNI CGCG 1 cut(s) 3552
BstH2I RGCGCY 2 cut(s) 277, 3424
BstHHI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 5027
BstMAI GTCTC 8 cut(s) 1359, 1674, 4505, 5010, 5100, 5127, 5383, 5810
BstNSI RCATGY 4 cut(s) 2705, 3501, 3686, 4613
BstSFI CTRYAG 8 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819, 5680
BstSLI GKGCMC 2 cut(s) 1182, 5105
BstUI CGCG 1 cut(s) 3552
BstV2I GAAGAC 2 cut(s) 582, 4045
BstX2I RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
BstXI CCANNNNNNTGG 7 cut(s) 4125, 4170, 4239, 4284, 4353, 4437, 4476
BstYI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
Bsu15I ATCGAT 1 cut(s) 92
BsuI GTATCC 4 cut(s) 617, 1040, 2208, 5727
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 2 cut(s) 4505, 5246
BtgZI GCGATG 1 cut(s) 1152
BtrI CACGTC 1 cut(s) 259
BtsIMutI CAGTG 9 cut(s) 268, 1437, 1465, 1702, 2415, 2992, 3327, 3921, 4518
CaiI CAGNNNCTG 1 cut(s) 2991
CciI TCATGA 1 cut(s) 73
CfoI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 5027
Cfr10I RCCGGY 5 cut(s) 22, 2121, 2492, 4931, 5228
ClaI ATCGAT 1 cut(s) 92
CspAI ACCGGT 1 cut(s) 22
CspCI CAANNNNNGTGG 4 cut(s) 2463, 2498, 3511, 3546
DraI TTTAAA 1 cut(s) 2965
DraIII CACNNNGTG 1 cut(s) 1349
DrdI GACNNNNNNGTC 1 cut(s) 1313
DseDI GACNNNNNNGTC 1 cut(s) 1313
EaeI YGGCCR 4 cut(s) 348, 1482, 1557, 2119
Eam1104I CTCTTC 5 cut(s) 982, 2584, 3744, 4956, 5321
EarI CTCTTC 5 cut(s) 982, 2584, 3744, 4956, 5321
EciI GGCGGA 1 cut(s) 5782
Ecl136II GAGCTC 3 cut(s) 904, 2178, 2635
Eco130I CCWWGG 8 cut(s) 897, 2116, 4095, 4209, 4323, 4505, 4623, 5246
Eco147I AGGCCT 2 cut(s) 1195, 1252
Eco24I GRGCYC 3 cut(s) 906, 2180, 2637
Eco31I GGTCTC 1 cut(s) 4505
Eco47I GGWCC 8 cut(s) 325, 1340, 1708, 2322, 4176, 4290, 4404, 5306
Eco47III AGCGCT 1 cut(s) 275
Eco53kI GAGCTC 3 cut(s) 904, 2178, 2635
Eco57I CTGAAG 9 cut(s) 290, 317, 594, 1812, 3248, 4923, 5040, 5737, 5751
Eco88I CYCGRG 2 cut(s) 155, 867
EcoICRI GAGCTC 3 cut(s) 904, 2178, 2635
EcoO109I RGGNCCY 2 cut(s) 325, 1708
EcoRI GAATTC 2 cut(s) 2231, 3935
EcoT14I CCWWGG 8 cut(s) 897, 2116, 4095, 4209, 4323, 4505, 4623, 5246
EcoT22I ATGCAT 5 cut(s) 1006, 3292, 4201, 4315, 5478
EcoT38I GRGCYC 3 cut(s) 906, 2180, 2637
ErhI CCWWGG 8 cut(s) 897, 2116, 4095, 4209, 4323, 4505, 4623, 5246
FalI AAGNNNNNCTT 8 cut(s) 915, 947, 2727, 2759, 3456, 3488, 5657, 5689
FauI CCCGC 4 cut(s) 989, 1666, 2788, 4936
FauNDI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
FbaI TGATCA 3 cut(s) 753, 829, 3406
FblI GTMKAC 1 cut(s) 4895
FriOI GRGCYC 3 cut(s) 906, 2180, 2637
GlaI GCGC 6 cut(s) 275, 1548, 3422, 3502, 3553, 5026
GsaI CCCAGC 6 cut(s) 3979, 4549, 4654, 4803, 4833, 5319
GsuI CTGGAG 5 cut(s) 1698, 2009, 3278, 3688, 5778
HaeII RGCGCY 2 cut(s) 277, 3424
HapII CCGG 6 cut(s) 23, 2122, 2493, 3854, 4932, 5229
HhaI GCGC 6 cut(s) 276, 1549, 3423, 3503, 3554, 5027
Hin6I GCGC 6 cut(s) 274, 1547, 3421, 3501, 3552, 5025
HinP1I GCGC 6 cut(s) 274, 1547, 3421, 3501, 3552, 5025
HpaII CCGG 6 cut(s) 23, 2122, 2493, 3854, 4932, 5229
Hpy99I CGWCG 3 cut(s) 2649, 3166, 4582
HpyCH4IV ACGT 4 cut(s) 258, 947, 1296, 5281
HpySE526I ACGT 4 cut(s) 258, 947, 1296, 5281
HspAI GCGC 6 cut(s) 274, 1547, 3421, 3501, 3552, 5025
KpnI GGTACC 1 cut(s) 1767
KroI GCCGGC 1 cut(s) 2121
KroNI GCCGGC 1 cut(s) 2123
Ksp22I TGATCA 3 cut(s) 753, 829, 3406
LguI GCTCTTC 1 cut(s) 4956
LmnI GCTCC 7 cut(s) 434, 901, 1420, 3707, 3830, 4443, 5419
MaeII ACGT 4 cut(s) 258, 947, 1296, 5281
MaeIII GTNAC 9 cut(s) 688, 813, 892, 1392, 2095, 2853, 2954, 2987, 3882
MbiI CCGCTC 2 cut(s) 1661, 2463
MfeI CAATTG 3 cut(s) 437, 822, 1553
MflI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
MhlI GDGCHC 6 cut(s) 906, 1182, 1676, 2180, 2637, 5105
MlsI TGGCCA 2 cut(s) 1484, 1559
MluNI TGGCCA 2 cut(s) 1484, 1559
MlyI GAGTC 8 cut(s) 1904, 2060, 2344, 2807, 4177, 4291, 4405, 5077
MmeI TCCRAC 8 cut(s) 25, 156, 1280, 2247, 3060, 4015, 4763, 4860
Mox20I TGGCCA 2 cut(s) 1484, 1559
Mph1103I ATGCAT 5 cut(s) 1006, 3292, 4201, 4315, 5478
MroNI GCCGGC 1 cut(s) 2121
MroXI GAANNNNTTC 3 cut(s) 964, 1797, 3957
MscI TGGCCA 2 cut(s) 1484, 1559
Msp20I TGGCCA 2 cut(s) 1484, 1559
MspA1I CMGCKG 3 cut(s) 1444, 3434, 3623
MspI CCGG 6 cut(s) 23, 2122, 2493, 3854, 4932, 5229
MunI CAATTG 3 cut(s) 437, 822, 1553
Mva1269I GAATGC 5 cut(s) 1371, 3796, 4792, 4831, 5735
MvnI CGCG 1 cut(s) 3552
NaeI GCCGGC 1 cut(s) 2123
NcoI CCATGG 2 cut(s) 4505, 5246
NdeI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
NgoMIV GCCGGC 1 cut(s) 2121
NheI GCTAGC 1 cut(s) 5725
NmeAIII GCCGAG 1 cut(s) 5743
NmuCI GTSAC 5 cut(s) 892, 1392, 2954, 2987, 3882
NsiI ATGCAT 5 cut(s) 1006, 3292, 4201, 4315, 5478
NspI RCATGY 4 cut(s) 2705, 3501, 3686, 4613
NspV TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5464
OliI CACNNNNGTG 1 cut(s) 261
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 1 cut(s) 73
PceI AGGCCT 2 cut(s) 1195, 1252
PciI ACATGT 2 cut(s) 3682, 4609
PciSI GCTCTTC 1 cut(s) 4956
PctI GAATGC 5 cut(s) 1371, 3796, 4792, 4831, 5735
PdiI GCCGGC 1 cut(s) 2123
PdmI GAANNNNTTC 3 cut(s) 964, 1797, 3957
PflMI CCANNNNNTGG 3 cut(s) 470, 4782, 5315
PfoI TCCNGGA 2 cut(s) 3939, 3961
PinAI ACCGGT 1 cut(s) 22
PleI GAGTC 8 cut(s) 1903, 2060, 2344, 2807, 4177, 4291, 4405, 5077
PpsI GAGTC 8 cut(s) 1903, 2060, 2344, 2807, 4177, 4291, 4405, 5077
PpuMI RGGWCCY 2 cut(s) 325, 1708
PscI ACATGT 2 cut(s) 3682, 4609
PshBI ATTAAT 1 cut(s) 1323
PsiI TTATAA 2 cut(s) 2033, 3537
Psp124BI GAGCTC 3 cut(s) 906, 2180, 2637
Psp5II RGGWCCY 2 cut(s) 325, 1708
PspFI CCCAGC 6 cut(s) 3975, 4545, 4650, 4799, 4829, 5315
PspPPI RGGWCCY 2 cut(s) 325, 1708
PsrI GAACNNNNNNTAC 2 cut(s) 2817, 2849
PstI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
PstNI CAGNNNCTG 1 cut(s) 2991
PsuI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
PvuII CAGCTG 3 cut(s) 1444, 3434, 3623
SacI GAGCTC 3 cut(s) 906, 2180, 2637
SapI GCTCTTC 1 cut(s) 4956
ScaI AGTACT 2 cut(s) 4558, 5750
SchI GAGTC 8 cut(s) 1904, 2060, 2344, 2807, 4177, 4291, 4405, 5077
SduI GDGCHC 6 cut(s) 906, 1182, 1676, 2180, 2637, 5105
SfcI CTRYAG 8 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819, 5680
Sfr274I CTCGAG 1 cut(s) 155
SfuI TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5464
SinI GGWCC 8 cut(s) 325, 1340, 1708, 2322, 4176, 4290, 4404, 5306
SlaI CTCGAG 1 cut(s) 155
SmlI CTYRAG 4 cut(s) 155, 615, 2326, 5136
SmoI CTYRAG 4 cut(s) 155, 615, 2326, 5136
SpeI ACTAGT 2 cut(s) 758, 4451
SseBI AGGCCT 2 cut(s) 1195, 1252
SspI AATATT 2 cut(s) 1087, 1526
SstI GAGCTC 3 cut(s) 906, 2180, 2637
StuI AGGCCT 2 cut(s) 1195, 1252
StyI CCWWGG 8 cut(s) 897, 2116, 4095, 4209, 4323, 4505, 4623, 5246
TaiI ACGT 4 cut(s) 261, 950, 1299, 5284
TaqII GACCGA 2 cut(s) 2310, 5771
TatI WGTACW 8 cut(s) 83, 1078, 3899, 4217, 4331, 4454, 4556, 5748
TauI GCSGC 2 cut(s) 1403, 2708
TscAI CASTG 9 cut(s) 268, 1437, 1465, 1702, 2415, 2992, 3327, 3921, 4525
TseFI GTSAC 5 cut(s) 892, 1392, 2954, 2987, 3882
Tsp45I GTSAC 5 cut(s) 892, 1392, 2954, 2987, 3882
TspGWI ACGGA 2 cut(s) 636, 4121
TspRI CASTG 9 cut(s) 268, 1437, 1465, 1702, 2415, 2992, 3327, 3921, 4525
Van91I CCANNNNNTGG 3 cut(s) 470, 4782, 5315
VpaK11BI GGWCC 8 cut(s) 325, 1340, 1708, 2322, 4176, 4290, 4404, 5306
VspI ATTAAT 1 cut(s) 1323
XceI RCATGY 4 cut(s) 2705, 3501, 3686, 4613
XcmI CCANNNNNNNNNTGG 2 cut(s) 589, 1477
XhoI CTCGAG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 4895
XmnI GAANNNNTTC 3 cut(s) 964, 1797, 3957
ZrmI AGTACT 2 cut(s) 4558, 5750
Zsp2I ATGCAT 5 cut(s) 1006, 3292, 4201, 4315, 5478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.