Rroxscaffold_159G00432580

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000159
OG0006658
Physical Location & Seq
Forward (+)
358 .. 8680
8323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_159G00432580.1

Sequence Viewer

Length: 2772 bp
ATGCTGTTACGGGAGTTAAATCTGGGAATGTATGAGATCCTTCAAAAATGCGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTTGGGAAGAAGATGAACATCGGTGACATTTTTAAAAGGACAGTTTTATCGTACAGGCATGGAATTTCTTTCAAATGGACAGGTGATCCTCGAATTTCGTCGGCGAACATAATTTGGATCAACTCAGAATCAACAACTTGGATAAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCATGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATGTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAGGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCGACAGCTGTTGCAATGGTGGCAAAAGGTGTTCTCAAAGAGCATCTTATTCTCTTGGCAAACAGTATGACATGCGCTGGAAATTTTGTTGGTTTCAATCCTGGTGGTTATAAAGCACTATCTCGCGCATTGAATATACAAACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCTTTGTCAAGCATAGTTGCATCATGTTCCTGGGGTAAACATGTAGCTGTTGGTACTGGCTCCAGATTCGAAATCCTCTGGGACACAAGAGAGGGAGGTTTGAATGAAGAGGGTGGGATAGATGTTTTCAACTTTCTACATATGGTGAATGCTGCAAATGGAGAAGAATCAACTACTGCAGCTTTGGGAGCAGAAATTGACGATCTTGTGCCGGAATATGAAAATGGAGATGTGTCGATGTCACCAGAACATAACTGTACTTCTGATAAGCCAGTGTTTGAAGATATTCTGGGATTCATCTCTCAACACCGCTTCTTCTGGTGGGAAAGACTGGGGGCAGTTAAAGTTGGAAGACAAGATAGCATTTCAGCAGAAACCCAAGTGGATAGCACATCTGCATGGGGGGCCAAGGCTATTACAGAAGATGCCCCATCTGTATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCATACAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGATGCCCCATCTGCATGGGGGACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACGGCCAAAACTGCTACATCTCCTTGGGAGACCAACACTGGTAGAGAAGATTCCACATCGGGCTGGGGGACAAGTACTGCCAGAGAAGATGCCACGACGGCATGGGGGAAAAGCAATGAAAAGAAACATGTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTTGCTGGGGGACAGAAAATCCACAATCTACATGGAGGACAGAAAGCGAGAATGGTTGGTCTGGTGGTGGGGTGAGTAAAGCTGAATCAACAGACGTCCAGTCCAAGAAAGCACAAGAAGATTCTACAGCCAATGGTTGGAATGCCTTTTCTGCTGGGCCAACAAAACCCCAAAAGGAGAATGCTGGGTCTGGATGGGGCATGAAAGGTTTTGATAAGAAAGATGGTAATGAGTTGGAACAAGATAAGTGTGCTGCCCGTCATTCTTGGGGGCAGCAAAAGCCCGCCGGTACTTCTCAGGGCCCTTGGGCACAACAAAAGTCCCCTGAGACTTCTAAGGGTGCTTGGGGGCAGCAAAATGCCGCTACTTCAGAAGGTGCTTGGGGTCAGGAAAAGCCTGCCGATACTTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCTGAGATATCTGAAGGCGCTTGGGGGAGCAAAAGCGTGCTGATGCTAATTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCAGATATTTCTGAAGGCGCATGGGGGCAGCAAAAGCCTGATACAACTTCTCAAGGCTCTTGGGGACAGCAAAAGTCTCCTGAAATGTCTCAGGCAAAAGTCTCCTGATATTTCTCAAGGCGCATGGGGGCAACAAAAGCCTTCTGATACTTCTCAACCTGCAACTGTGAATCAATGGGATCCTCCAAGTGCAGGAGATGGAAGTGCCGGTGAAAGACAGCAACAATGGGGTCATAATGGAGATGCGAACAAGAAAAGACGTTTTGAAGGTGGCAGGAGTTGGGGTCCAAATGGTGGGGAGTGGAAGAGTAAGAATAGTCGCCCTGCTAATGCTGATTCTAGTGTGGCTGCACTATATACCGCAACGAGACAAAGATTGGATATTTTCACTTCTGAGGAGCAACATGTTCTTTCCCATATTGAGCCGATTATGCTGTCTATTCGAAGAATAATGCATCAATCTGGCTACAACGATGGAGATCGGTTGTCAGATGAGGATCAATCATACATACTTGAAAATGTGTTCACACACCACCCAGATAAAGATGTTAAGATGGGTTCTGGAATTGACTATTTTACGGTTGATAGGCATGGAAGTTTTCAAGAAAGCCGATGCCTTTTCGTCATATCAACTGATGGTCGCAAGGAGGACTTTTCCTACAGAAAATGCCTGCAAAATATGATAAAGGGAAAGTATCCTGAGCTAGCTGAAGCATTCAATGACAAGTACTTCAGCAAACCTCGGCGGAATCCCATGTTCGGTCAGACCCCTCCAGAGAGAACTGGGAATGAGGAGACACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

923

Amino Acids

101.19

Weight (kDa)

6.59

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 805 - 880 1.4e-27 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
AatII GACGTC 1 cut(s) 1548
Acc36I ACCTGC 1 cut(s) 2134
AccB7I CCANNNNNTGG 2 cut(s) 1587, 2261
AccII CGCG 2 cut(s) 449, 573
AciI CCGC 5 cut(s) 946, 1734, 1812, 2328, 2713
AclWI GGATC 6 cut(s) 31, 164, 209, 2141, 2154, 2472
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 3 cut(s) 147, 177, 529
AcuI CTGAAG 6 cut(s) 269, 1803, 1920, 2000, 2683, 2697
AcyI GRCGYC 1 cut(s) 1545
AfaI GTAC 8 cut(s) 137, 691, 895, 1222, 1261, 1363, 1741, 2696
AfiI CCNNNNNNNGG 4 cut(s) 1587, 2159, 2261, 2726
AflIII ACRYGT 3 cut(s) 676, 1414, 2371
AhdI GACNNNNNGTC 1 cut(s) 1549
AhlI ACTAGT 1 cut(s) 1256
AjnI CCWGG 3 cut(s) 362, 547, 665
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 7 cut(s) 455, 617, 683, 818, 1532, 2671, 2675
AluI AGCT 7 cut(s) 455, 617, 683, 818, 1532, 2671, 2675
Alw26I GTCTC 9 cut(s) 1310, 1772, 1890, 1970, 2048, 2060, 2073, 2329, 2756
AlwI GGATC 6 cut(s) 31, 164, 209, 2141, 2154, 2472
AoxI GGCC 4 cut(s) 1041, 1290, 1607, 1750
ApaI GGGCCC 1 cut(s) 1754
ApeKI GCWGC 8 cut(s) 614, 788, 815, 1703, 1723, 1801, 1995, 2315
ApoI RAATTY 3 cut(s) 147, 177, 529
Asp700I GAANNNNTTC 1 cut(s) 921
AspLEI GCGC 6 cut(s) 444, 524, 575, 1907, 1987, 2090
AspS9I GGNCC 8 cut(s) 1041, 1125, 1209, 1248, 1607, 1750, 1751, 2252
AsuHPI GGTGA 8 cut(s) 119, 179, 266, 317, 793, 870, 1534, 2189
AsuII TTCGAA 2 cut(s) 705, 2410
AsuNHI GCTAGC 1 cut(s) 2671
AvaII GGWCC 4 cut(s) 1125, 1209, 1248, 2252
BaeGI GKGCMC 2 cut(s) 1754, 1762
BamHI GGATCC 1 cut(s) 2146
BanII GRGCYC 1 cut(s) 1754
BbsI GAAGAC 1 cut(s) 994
BbvI GCAGC 8 cut(s) 626, 775, 827, 1690, 1735, 1813, 2007, 2302
BceAI ACGGC 4 cut(s) 1305, 1401, 1892, 1972
BciT130I CCWGG 3 cut(s) 364, 549, 667
BciVI GTATCC 1 cut(s) 2673
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 9 cut(s) 1310, 1772, 1890, 1970, 2048, 2060, 2073, 2329, 2756
BcuI ACTAGT 1 cut(s) 1256
BfaI CTAG 4 cut(s) 1257, 1434, 2307, 2672
BfmI CTRYAG 3 cut(s) 813, 1575, 2626
BfoI RGCGCY 2 cut(s) 445, 1908
BfuAI ACCTGC 1 cut(s) 2134
BfuI GTATCC 1 cut(s) 2673
BglI GCCNNNNNGGC 1 cut(s) 1385
BisI GCNGC 9 cut(s) 615, 789, 816, 1704, 1724, 1802, 1812, 1996, 2316
BlsI GCNGC 9 cut(s) 616, 790, 817, 1705, 1725, 1803, 1813, 1997, 2317
BmcAI AGTACT 2 cut(s) 1363, 2696
Bme1390I CCNGG 3 cut(s) 364, 549, 667
Bme18I GGWCC 4 cut(s) 1125, 1209, 1248, 2252
BmeRI GACNNNNNGTC 1 cut(s) 1549
BmgT120I GGNCC 8 cut(s) 1041, 1125, 1209, 1248, 1607, 1750, 1751, 2252
BmiI GGNNCC 8 cut(s) 697, 1042, 1126, 1210, 1249, 1752, 2148, 2253
BmrFI CCNGG 3 cut(s) 364, 549, 667
BmrI ACTGGG 2 cut(s) 977, 2760
BmtI GCTAGC 1 cut(s) 2675
BmuI ACTGGG 2 cut(s) 977, 2760
BpiI GAAGAC 1 cut(s) 994
BpmI CTGGAG 4 cut(s) 299, 682, 1952, 2724
Bpu10I CCTNAGC 1 cut(s) 2667
Bpu14I TTCGAA 2 cut(s) 705, 2410
BpuEI CTTGAG 2 cut(s) 2003, 2067
BsaBI GATNNNNATC 3 cut(s) 101, 2445, 2463
BsaHI GRCGYC 1 cut(s) 1545
BsaI GGTCTC 1 cut(s) 1310
BsaJI CCNNGG 6 cut(s) 666, 1044, 1310, 1428, 1754, 2708
BsaXI ACNNNNNCTCC 3 cut(s) 2236, 2266, 2753
Bsc4I CCNNNNNNNGG 4 cut(s) 1587, 2159, 2261, 2726
Bse118I RCCGGY 2 cut(s) 1736, 2174
Bse1I ACTGG 7 cut(s) 341, 697, 908, 972, 1330, 1549, 2755
Bse3DI GCAATG 2 cut(s) 468, 1408
Bse8I GATNNNNATC 3 cut(s) 101, 2445, 2463
BseBI CCWGG 3 cut(s) 364, 549, 667
BseDI CCNNGG 6 cut(s) 666, 1044, 1310, 1428, 1754, 2708
BseGI GGATG 2 cut(s) 274, 1649
BseJI GATNNNNATC 3 cut(s) 101, 2445, 2463
BseLI CCNNNNNNNGG 4 cut(s) 1587, 2159, 2261, 2726
BseMI GCAATG 2 cut(s) 468, 1408
BseMII CTCAG 9 cut(s) 222, 1760, 1767, 1874, 1881, 1954, 2071, 2352, 2658
BseNI ACTGG 7 cut(s) 341, 697, 908, 972, 1330, 1549, 2755
BseRI GAGGAG 1 cut(s) 2378
BseSI GKGCMC 2 cut(s) 1754, 1762
BseXI GCAGC 8 cut(s) 626, 775, 827, 1690, 1735, 1813, 2007, 2302
BseYI CCCAGC 4 cut(s) 1350, 1455, 1604, 1634
BsgI GTGCAG 3 cut(s) 416, 2178, 2301
Bsh1236I CGCG 2 cut(s) 449, 573
BshFI GGCC 4 cut(s) 1043, 1292, 1609, 1752
BsiSI CCGG 3 cut(s) 848, 1737, 2175
BslI CCNNNNNNNGG 4 cut(s) 1587, 2159, 2261, 2726
BsmAI GTCTC 9 cut(s) 1310, 1772, 1890, 1970, 2048, 2060, 2073, 2329, 2756
BsmI GAATGC 4 cut(s) 790, 1597, 1636, 2681
BsnI GGCC 4 cut(s) 1043, 1292, 1609, 1752
Bso31I GGTCTC 1 cut(s) 1310
Bsp119I TTCGAA 2 cut(s) 705, 2410
Bsp120I GGGCCC 1 cut(s) 1750
Bsp1286I GDGCHC 2 cut(s) 1754, 1762
Bsp143I GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 5 cut(s) 946, 1734, 1812, 2328, 2713
BspANI GGCC 4 cut(s) 1043, 1292, 1609, 1752
BspCNI CTCAG 9 cut(s) 221, 1759, 1768, 1873, 1882, 1953, 2070, 2353, 2659
BspFNI CGCG 2 cut(s) 449, 573
BspLI GGNNCC 8 cut(s) 697, 1042, 1126, 1210, 1249, 1752, 2148, 2253
BspMAI CTGCAG 1 cut(s) 817
BspMI ACCTGC 1 cut(s) 2134
BspOI GCTAGC 1 cut(s) 2675
BspPI GGATC 6 cut(s) 31, 164, 209, 2141, 2154, 2472
BspT104I TTCGAA 2 cut(s) 705, 2410
BspTNI GGTCTC 1 cut(s) 1310
BsrDI GCAATG 2 cut(s) 468, 1408
BsrFI RCCGGY 2 cut(s) 1736, 2174
BsrI ACTGG 7 cut(s) 341, 697, 908, 972, 1330, 1549, 2755
BssAI RCCGGY 2 cut(s) 1736, 2174
BssECI CCNNGG 6 cut(s) 666, 1044, 1310, 1428, 1754, 2708
BssMI GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
BssNI GRCGYC 1 cut(s) 1545
BssT1I CCWWGG 4 cut(s) 1044, 1310, 1428, 1754
Bst2UI CCWGG 3 cut(s) 364, 549, 667
Bst4CI ACNGT 6 cut(s) 127, 512, 893, 2134, 2548, 2769
Bst6I CTCTTC 2 cut(s) 738, 2267
BstACI GRCGYC 1 cut(s) 1545
BstBI TTCGAA 2 cut(s) 705, 2410
BstC8I GCNNGC 5 cut(s) 1734, 1848, 1925, 2639, 2673
BstF5I GGATG 2 cut(s) 274, 1649
BstFNI CGCG 2 cut(s) 449, 573
BstH2I RGCGCY 2 cut(s) 445, 1908
BstHHI GCGC 6 cut(s) 444, 524, 575, 1907, 1987, 2090
BstKTI GATC 8 cut(s) 39, 172, 204, 430, 841, 2149, 2449, 2467
BstMAI GTCTC 9 cut(s) 1310, 1772, 1890, 1970, 2048, 2060, 2073, 2329, 2756
BstMBI GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
BstNI CCWGG 3 cut(s) 364, 549, 667
BstNSI RCATGY 4 cut(s) 522, 680, 1418, 2375
BstSCI CCNGG 3 cut(s) 362, 547, 665
BstSFI CTRYAG 3 cut(s) 813, 1575, 2626
BstSLI GKGCMC 2 cut(s) 1754, 1762
BstUI CGCG 2 cut(s) 449, 573
BstV1I GCAGC 8 cut(s) 626, 775, 827, 1690, 1735, 1813, 2007, 2302
BstV2I GAAGAC 1 cut(s) 994
BstX2I RGATCY 2 cut(s) 36, 2146
BstXI CCANNNNNNTGG 6 cut(s) 1074, 1119, 1158, 1203, 1242, 1281
BstYI RGATCY 2 cut(s) 36, 2146
BsuI GTATCC 1 cut(s) 2673
BsuRI GGCC 4 cut(s) 1043, 1292, 1609, 1752
BtsCI GGATG 2 cut(s) 274, 1649
BtsIMutI CAGTG 3 cut(s) 348, 915, 1323
BtuMI TCGCGA 1 cut(s) 449
BveI ACCTGC 1 cut(s) 2134
Cac8I GCNNGC 5 cut(s) 1734, 1848, 1925, 2639, 2673
CfoI GCGC 6 cut(s) 444, 524, 575, 1907, 1987, 2090
Cfr10I RCCGGY 2 cut(s) 1736, 2174
Cfr13I GGNCC 8 cut(s) 1041, 1125, 1209, 1248, 1607, 1750, 1751, 2252
Csp6I GTAC 8 cut(s) 136, 690, 894, 1221, 1260, 1362, 1740, 2695
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviQI GTAC 8 cut(s) 136, 690, 894, 1221, 1260, 1362, 1740, 2695
DpnI GATC 8 cut(s) 38, 171, 203, 429, 840, 2148, 2448, 2466
DpnII GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
DraI TTTAAA 1 cut(s) 118
DriI GACNNNNNGTC 1 cut(s) 1549
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 2 cut(s) 738, 2267
Eam1105I GACNNNNNGTC 1 cut(s) 1549
EarI CTCTTC 2 cut(s) 738, 2267
EciI GGCGGA 1 cut(s) 2728
Eco130I CCWWGG 4 cut(s) 1044, 1310, 1428, 1754
Eco24I GRGCYC 1 cut(s) 1754
Eco31I GGTCTC 1 cut(s) 1310
Eco32I GATATC 1 cut(s) 1896
Eco47I GGWCC 4 cut(s) 1125, 1209, 1248, 2252
Eco57I CTGAAG 6 cut(s) 269, 1803, 1920, 2000, 2683, 2697
EcoO109I RGGNCCY 2 cut(s) 1750, 1751
EcoRII CCWGG 3 cut(s) 362, 547, 665
EcoRV GATATC 1 cut(s) 1896
EcoT14I CCWWGG 4 cut(s) 1044, 1310, 1428, 1754
EcoT22I ATGCAT 2 cut(s) 313, 2424
EcoT38I GRGCYC 1 cut(s) 1754
ErhI CCWWGG 4 cut(s) 1044, 1310, 1428, 1754
FalI AAGNNNNNCTT 4 cut(s) 477, 509, 2603, 2635
FauI CCCGC 1 cut(s) 1741
FauNDI CATATG 3 cut(s) 379, 630, 777
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 9 cut(s) 615, 789, 816, 1704, 1724, 1802, 1812, 1996, 2316
FokI GGATG 2 cut(s) 281, 1656
FriOI GRGCYC 1 cut(s) 1754
Fsp4HI GCNGC 9 cut(s) 615, 789, 816, 1704, 1724, 1802, 1812, 1996, 2316
FspBI CTAG 4 cut(s) 1257, 1434, 2307, 2672
GlaI GCGC 6 cut(s) 443, 523, 574, 1906, 1986, 2089
GluI GCNGC 9 cut(s) 615, 789, 816, 1704, 1724, 1802, 1812, 1996, 2316
GsaI CCCAGC 4 cut(s) 1354, 1459, 1608, 1638
GsuI CTGGAG 4 cut(s) 299, 682, 1952, 2724
HaeII RGCGCY 2 cut(s) 445, 1908
HaeIII GGCC 4 cut(s) 1043, 1292, 1609, 1752
HapII CCGG 3 cut(s) 848, 1737, 2175
HhaI GCGC 6 cut(s) 444, 524, 575, 1907, 1987, 2090
Hin1I GRCGYC 1 cut(s) 1545
Hin6I GCGC 6 cut(s) 442, 522, 573, 1905, 1985, 2088
HinP1I GCGC 6 cut(s) 442, 522, 573, 1905, 1985, 2088
HpaII CCGG 3 cut(s) 848, 1737, 2175
HphI GGTGA 8 cut(s) 119, 179, 266, 317, 793, 870, 1534, 2189
Hpy166II GTNNAC 2 cut(s) 674, 2493
Hpy8I GTNNAC 2 cut(s) 674, 2493
Hpy99I CGWCG 2 cut(s) 187, 1387
HpyAV CCTTC 9 cut(s) 50, 76, 244, 347, 1817, 1895, 1975, 2118, 2228
HpyCH4III ACNGT 6 cut(s) 127, 512, 893, 2134, 2548, 2769
HpyCH4IV ACGT 2 cut(s) 1545, 2227
HpySE526I ACGT 2 cut(s) 1545, 2227
Hsp92I GRCGYC 1 cut(s) 1545
HspAI GCGC 6 cut(s) 442, 522, 573, 1905, 1985, 2088
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
LmnI GCTCC 4 cut(s) 701, 824, 1914, 2365
Lsp1109I GCAGC 8 cut(s) 626, 775, 827, 1690, 1735, 1813, 2007, 2302
MaeI CTAG 4 cut(s) 1257, 1434, 2307, 2672
MaeII ACGT 2 cut(s) 1545, 2227
MaeIII GTNAC 3 cut(s) 6, 107, 876
MalI GATC 8 cut(s) 38, 171, 203, 429, 840, 2148, 2448, 2466
MboI GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
MflI RGATCY 2 cut(s) 36, 2146
MhlI GDGCHC 2 cut(s) 1754, 1762
MlyI GAGTC 2 cut(s) 1126, 1210
MmeI TCCRAC 3 cut(s) 964, 1568, 1665
Mph1103I ATGCAT 2 cut(s) 313, 2424
MroXI GAANNNNTTC 1 cut(s) 921
MseI TTAA 5 cut(s) 17, 117, 294, 978, 2517
MslI CAYNNNNRTG 7 cut(s) 1033, 1072, 1117, 1156, 1201, 1240, 1279
MspA1I CMGCKG 2 cut(s) 455, 617
MspI CCGG 3 cut(s) 848, 1737, 2175
MspR9I CCNGG 3 cut(s) 364, 549, 667
Mva1269I GAATGC 4 cut(s) 790, 1597, 1636, 2681
MvaI CCWGG 3 cut(s) 364, 549, 667
MvnI CGCG 2 cut(s) 449, 573
NdeI CATATG 3 cut(s) 379, 630, 777
NdeII GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
NheI GCTAGC 1 cut(s) 2671
NlaIV GGNNCC 8 cut(s) 697, 1042, 1126, 1210, 1249, 1752, 2148, 2253
NmeAIII GCCGAG 1 cut(s) 2689
NmuCI GTSAC 2 cut(s) 107, 876
NruI TCGCGA 1 cut(s) 449
NsiI ATGCAT 2 cut(s) 313, 2424
NspI RCATGY 4 cut(s) 522, 680, 1418, 2375
NspV TTCGAA 2 cut(s) 705, 2410
PciI ACATGT 3 cut(s) 676, 1414, 2371
PctI GAATGC 4 cut(s) 790, 1597, 1636, 2681
PdmI GAANNNNTTC 1 cut(s) 921
PflMI CCANNNNNTGG 2 cut(s) 1587, 2261
PkrI GCNGC 9 cut(s) 616, 790, 817, 1705, 1725, 1803, 1813, 1997, 2317
PleI GAGTC 2 cut(s) 1126, 1210
PpsI GAGTC 2 cut(s) 1126, 1210
PscI ACATGT 3 cut(s) 676, 1414, 2371
PsiI TTATAA 1 cut(s) 558
Psp6I CCWGG 3 cut(s) 362, 547, 665
PspFI CCCAGC 4 cut(s) 1350, 1455, 1604, 1634
PspGI CCWGG 3 cut(s) 362, 547, 665
PspN4I GGNNCC 8 cut(s) 697, 1042, 1126, 1210, 1249, 1752, 2148, 2253
PspOMI GGGCCC 1 cut(s) 1750
PspPI GGNCC 8 cut(s) 1041, 1125, 1209, 1248, 1607, 1750, 1751, 2252
PstI CTGCAG 1 cut(s) 817
PsuI RGATCY 2 cut(s) 36, 2146
PvuII CAGCTG 2 cut(s) 455, 617
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 8 cut(s) 137, 691, 895, 1222, 1261, 1363, 1741, 2696
RsaNI GTAC 8 cut(s) 136, 690, 894, 1221, 1260, 1362, 1740, 2695
RseI CAYNNNNRTG 7 cut(s) 1033, 1072, 1117, 1156, 1201, 1240, 1279
SaqAI TTAA 5 cut(s) 17, 117, 294, 978, 2517
SatI GCNGC 9 cut(s) 615, 789, 816, 1704, 1724, 1802, 1812, 1996, 2316
Sau3AI GATC 8 cut(s) 36, 169, 201, 427, 838, 2146, 2446, 2464
Sau96I GGNCC 8 cut(s) 1041, 1125, 1209, 1248, 1607, 1750, 1751, 2252
ScaI AGTACT 2 cut(s) 1363, 2696
SchI GAGTC 2 cut(s) 1126, 1210
ScrFI CCNGG 3 cut(s) 364, 549, 667
SduI GDGCHC 2 cut(s) 1754, 1762
SfcI CTRYAG 3 cut(s) 813, 1575, 2626
SfuI TTCGAA 2 cut(s) 705, 2410
SinI GGWCC 4 cut(s) 1125, 1209, 1248, 2252
SmiMI CAYNNNNRTG 7 cut(s) 1033, 1072, 1117, 1156, 1201, 1240, 1279
SmlI CTYRAG 2 cut(s) 2018, 2082
SmoI CTYRAG 2 cut(s) 2018, 2082
SpeI ACTAGT 1 cut(s) 1256
SsiI CCGC 5 cut(s) 946, 1734, 1812, 2328, 2713
SspMI CTAG 4 cut(s) 1257, 1434, 2307, 2672
StyD4I CCNGG 3 cut(s) 362, 547, 665
StyI CCWWGG 4 cut(s) 1044, 1310, 1428, 1754
TaaI ACNGT 6 cut(s) 127, 512, 893, 2134, 2548, 2769
TaiI ACGT 2 cut(s) 1548, 2230
TaqI TCGA 4 cut(s) 175, 705, 872, 2410
TaqII GACCGA 1 cut(s) 2717
TatI WGTACW 4 cut(s) 893, 1259, 1361, 2694
TauI GCSGC 1 cut(s) 1814
Tru1I TTAA 5 cut(s) 17, 117, 294, 978, 2517
Tru9I TTAA 5 cut(s) 17, 117, 294, 978, 2517
TscAI CASTG 3 cut(s) 348, 915, 1330
TseFI GTSAC 2 cut(s) 107, 876
TseI GCWGC 8 cut(s) 614, 788, 815, 1703, 1723, 1801, 1995, 2315
Tsp45I GTSAC 2 cut(s) 107, 876
TspDTI ATGAA 8 cut(s) 57, 113, 338, 756, 870, 922, 1419, 1667
TspRI CASTG 3 cut(s) 348, 915, 1330
Van91I CCANNNNNTGG 2 cut(s) 1587, 2261
VpaK11BI GGWCC 4 cut(s) 1125, 1209, 1248, 2252
XapI RAATTY 3 cut(s) 147, 177, 529
XceI RCATGY 4 cut(s) 522, 680, 1418, 2375
XcmI CCANNNNNNNNNTGG 1 cut(s) 1479
XmnI GAANNNNTTC 1 cut(s) 921
XspI CTAG 4 cut(s) 1257, 1434, 2307, 2672
ZraI GACGTC 1 cut(s) 1546
ZrmI AGTACT 2 cut(s) 1363, 2696
Zsp2I ATGCAT 2 cut(s) 313, 2424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.