Rh1BG051200

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006658
Physical Location & Seq
Forward (+)
7028503 .. 7043634
15132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG051200.1

Sequence Viewer

Length: 5535 bp
ATGGAGGAAGCCTCTTGTTCACCGGTTTTAGATGGGGAGTTAGTTGGAATTAAATTTGGTTTGGCAACTCATCATGAAATTTGTACAGCATCGATGATGGGATGCTCCATTAGCCATGCCAGTCAGCTTTCCAACCCATTTCTTGGCCTTCCTCTCGAGTTTGGGAAGTGTGAATCTTGTGGCACTTCCGAACCTGGAAAGTGCGAAGGGCATTTTGGATATATCAACTTACCATTACCGATATATCATCCTAACCATGTCAGTGAGCTGAAGCGCTTGCTGAGTTTTTTGTGCCTGAAGTGCTTAAAAATGAAAAAGAACAAGGTCCCAAGCAAGAGCGTTGGTTTGGCCGAAAGATTGTTATCTGTATGTTGTGAGGATGCTTCACAAGTTTCTATCGCGGAGATTAAACCTACAGATGGGGCTTGCTCCTTGCAATTGAAACTACCTTCTAATAAAAAACCACCGCCTGGTTTTTGGAATTTCCTAGAAAGATATGGTTTTCGTTATGGTGATGGCGTGAAACGCACTTTGCTTCCTAGAGAGGTGATGCAGATTCTCAAAAGAATCCCTGAAGACACCAGAAGAAAACTGGCTGCTAAAGGCTATAATCCTCAAGACGGATACATCTTGAATCATATACCTGTCCCTCCTAATTGCTTGTCTGTGCCTGAAATATCTGATGGTGTTACTGTTATGTCTGCGGATCCTTCAATATCAATGCTCAAAAAAGTTCTAAGGCAAGTTGAAATGATCACTAGTTCAAGGTCTGGTACAGCAAATTTTGAATCTGAAATTGTTGAGGCGAATGAATTACAAGCAATTGTTGATCAGTATCTTCAAGTTAGGGGTACTGGAAAGGCATCCCGAGATATTGATGCAAGATTTGGAGTGACCAAGGAGCTCAACGATTCTTCAACAAAAGCATGGCTTGAAAAGATGAGGACATTGTTCATCAGAAAGGGTTCAGGCTTCTCTTCCCGCACAGTAATTACTGGGGATGCATATAGAAGGGTGAATGAAGTTGGTATCCCATATGAAATTGCTCAAAGAATAACATTCGAGGAGAAGGTCAATGTACATAATATTAGGCGTCTGCAAGAGCTGGTAGATAGCAAGTTATGCCTAACCTACAGCGATGGTTCATCAACTTACTCTTTAAGGGAAGGTTCAAAGGGGCACACATTTCTGAGGCCTGGTCAAGTGGTGCATAGGAGGATAATGGATGGGGATCTTGTTTTCGTAAATAGGCCTCCAACTACCCATAAACATTCGCTTCAAGCACTTCAAGTTTACGTCCATGATGACCATGTGGTCAAGATTAATCCTCTAATATGTGGACCACTAAGTGCTGACTTTGATGGAGACTGCATTCATTTGTTCTATCCCCAGTCACTTGCTGCAAAGGCAGAAGTTATGGAGCTTTTTTCAGTGGAGAAACAGCTGCTTAGTTCTCACAGTGGCAAGCCCAATTTGCAACTGGCCACCGATTCACTTTTGTCATTAAAAATGATGTTTAAGAAATATTTTTTCGACAAAGCAGCAGCGCAACAATTGGCCATGTTTGTTTCATCTTCATTGCCACAACCAGCTTTATTGAAAGCTAATTCTGCAGTTCCATATTGGACTGCTTTGCAGATATTGCAGACTGCATTGCCTGCTCATTTTGAGTGCTCTGGAGACAGGCACTTGGTCAGTGCTAGTGAGGTCCTCAAACTTGATTGTAATACAAGTCTTATACCAGCTCTTATCAATGACATTGGTACCTCTATTTTCTTCGAGAAAAGTGCTGAAGATGTTCTCAATTTCTTTAATTCTATGCAGCCATTGCTGATGGAGAATTTATTTTCAGAAGGGTTCAGTGTAGGTTTGGAGGATTTTGCCATGTCTAGGGAGTCCATTCAGGAGATTAGAAAGGGACTTAAAATCATTTCTCCTTTGCTGTATCATATGAGGACAACATATAATGAGCTTGTTGAGTTGCAACTGGAGAATCACATTCGAACGGTGAAACAACCAGTTTCGAATTTTATATCAAATTCATCGGCATTGGGTGATTTAATTGACTCTAAGAGTGATTCAGCCATGAACAAAGTAACTCAACAAGTTGGGTTCCTTGGCCGGCAACTTTATGACCAGGGGAAACTCTACTCAAAAACATTGGTTGAGGATGTGAGCTCTCTGTATCAGAGCAAGTATCCATCTGACAGTGTGGATTATCCCTCTGCAGAATTCGGATTAGTTCATAGTGGTTTTGTTCACGGGTTGGATCCTGTTGAGGGAATGATTCATTCCATTGCTACAAGGGAAGTAATTGTTCGGTCCTCAAGAGGGTTGTCTGAACCTGGGACTCTATTCAAAAATCTAATGGCTACTCTCCGAGATGTTGTCATTTGCTATGATGGCACAGTGAGGAATGTGTGCAGTAATTCTATAATTCAGTTTGAGTATGGAGTAAAGAGTGGATCTAGTCCACAAAATTTGTTCCCTGCCGGTGAACCTGTGGGTGTGTTAGCTGCAACAGCAATGTCAAATCCAGCATACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCTTCATGGGATCTGATGAAGGAAATACTGCTCTGCAAAGTCAGTTTCAAGAACGAGCTCATTGATCGTCGTGTAATCTTGTATCTGAATGACTGTGGTTGCGGAAGAAACTACTGCAGAGAACATGCGGCATATTTGGTCAAGAATCAGTTGAAAAAAGTCAGCCTTAAAGATACTGCAGTAGAGTTTATGATTGAATATCAAAATCAGCGAGCTGCGGGAAGCATGGAAATTGACTCTGGCCTTGTTGGTCATGTTCATTTGAATGAGATGCTGTTACGGGAGTTAAATCTGGGAATGTATGAGATCCTTCAAAAATGTGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTTGGGAAGAAGATGAACATCGGTGACATTTTTAAAAGGACAGTTTTATCGTACAGTGAATGTTGTTCTTTTCATCAATCCTCTGCTGACAATGGATCTGGATCTCCTTGCTTGATGTTCTTCTACCAAGATTTTAATAATTCAGAGTTGGAGGCCATTTCACAAATCTTGGCTGACTTTATTTGCCCAGTTCTTCTAAAAACAGTAATTAAAGGTGATCCTCGAATTTCGTCGGCGAACATAATTTGGATCAACTCAGAATCAACAACTTGGATAAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCATGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATGTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAAGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCGACAGCTGTTGCAATGGTGGCAAAAGGTGTTCTCAAAGAGCATCTTATTCTCTTGGCAAACAGTATGACATGCGCTGGAAATTTCGTTGGCTTCAATCCTGGTGGTTATAAAGCACTATCTCGCGCACTGAATATACAAGTACCCTTTACAGAAGCAACATTGTTTACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCTTTGTCGAGCATAGTTGCATCATGTTCCTGGGGTAAACATGTAGCTGTTGGTACTGGCTCCAGATTCGAAATCCTCTGGGACACAAGAGAGGGAGGTTTGAACGAAGAGGGTGGGATAGATGTTTTCAACTTTCTACATATGGTGAATGCTGCAAATGGAGAAGAATCAACTACTGCAGCTTTGGGAGCAGAAATTGATGATCTTGTGCCGGAATATGAAAATGGAGATGTGTCCTTGTCACCAGAACATAACTGTACTTCTGATAAGCCAGTGTTTGAAGATATTGTTGAATTCCCGGATAGTTTCGAAAATGTTCCTGGAAAATCAAGCTGGGATTCATCTCTCAACACTGCTTCTTCTGGTGGAAAAGACTGGGGGGCAGTTAAAGTTGGAAGACAAGATAGCATTTCAGCAGAAACCCAAGTGGATAGCACATCTGCATGGGGGGCCAAGGTTAGTACAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACATCTCCATGGGAGACCAACACTGGTAGAGAAGATTCCACATCGGGCTGGGGGACAAGTACTGCCAGAGAAGATGCCACGACGGCATGGGGAAAAAGCAATGAAAAGAAACATGTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTTGCTGGGGGACAGAAAATCCACAATCTACATGGAGGACTAGTACAGAAAGCGAGAATGGTTGGTCTGGTGGTGGGGTGAGTAAAGCTGAATCAACAGATGTCCAGTCCAAGAAAGCACAAGAAGATTCTACATCCAATGGTTGGAATGCCTTTTCTGGTGGGCCAACAAAACCCCAAAAGGAGAATGCTGGGTCTGGATGGGGCATGAAAGGTTTTGAGAAGAAAGATGGTAATGAGTTGGAACAAGATAAGTCTACTGCCCGTCATTCTTGGGGGCAGCAAAAGCCCGCTGGTACTTCTCAGGGTCCTTGGGCACAGCAAAAGTCCCCTGAGATTTCTAAAGGTGCTTGGGGGCAGCAAAATGCCGCTACTTCAGAAGGTGCTTGGGGTCAAGAAAAGCCTGCCGATACTTCTCAGGGCAGTTGGGGACGGCAAAAGTCGCCTGAGATTTCTAAAGGCGCTTGGCAGGAGCAAAAGCCTGCTGATGCTAATTCTCAGAGCACTTGGGGACGGCAAAAGTCTCCTGATATTTCTGAAGGCGCATGGGGGCAGCAAAAGCCTGATACAACTTCTCAAGGCTCTTGGGGACAGCAAAAGTCTCCTGAGATGTCTCAGGGCACTTGGGGAAGGCAAAAGTCTCCTGATATTTCACAAGGCGCATGGGGGCAACAAAAGCCTTCTGATACTTCTCAACCTGCAACTGTGAATCAATGGGATTCTCCAAGTGCAGGAGATGGAAGTGCCAGTGAAAGACAGCAACAATGGGGTCATAATGGAGATGCAAACAAGAAAAGACGTTTTGAAGGTGGCAGGAGTTGGGGTCCAAATGCTGGGGAGTGGAAGAGTAAGAATAGTCGCCCTGCTAATGCTGATTCTAGTGTGGCTGCACTATATACCGCAACGAGACAAAGATTGGATATTTTCACTTCTGAGGAGCAACATGTTCTTTCCCATATTGAGCCGATTATGCTGTCTATTCGAAGAATAATGCATCAATCTGGCTACAATGATGGAGATCGGTTGTCAGATGAGGATCAATCATACATACTTGAAAATGTGTTCACACACCACCCAGATAAAGATGTTAAGATGGGTTCTGGAATTGACTATTTTACAGTAAGATTGCTTACTCTGTTCTTGCCATACCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1844

Amino Acids

202.75

Weight (kDa)

6.07

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 48 - 281 3.1e-10 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 324 - 478 4.9e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 481 - 626 6.1e-10 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 755 - 1181 7.2e-10 RNA polymerase Rpb1, domain 5
DUF3223 PF11523 1788 - 1834 7.1e-10 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3537
AasI GACNNNNNNGTC 1 cut(s) 1313
Acc36I ACCTGC 1 cut(s) 5083
Acc65I GGTACC 1 cut(s) 1763
AccB1I GGYRCC 1 cut(s) 1763
AccB7I CCANNNNNTGG 4 cut(s) 143, 470, 4521, 5210
AccI GTMKAC 1 cut(s) 4634
AccII CGCG 3 cut(s) 401, 3428, 3552
AcoI YGGCCR 4 cut(s) 348, 1482, 1557, 2119
AcuI CTGAAG 7 cut(s) 290, 317, 594, 1812, 3248, 4737, 4935
AcyI GRCGYC 1 cut(s) 1093
AdeI CACNNNGTG 1 cut(s) 1349
AfeI AGCGCT 1 cut(s) 275
AflIII ACRYGT 3 cut(s) 3682, 4342, 5320
AgeI ACCGGT 1 cut(s) 22
AhlI ACTAGT 3 cut(s) 758, 4184, 4418
AjnI CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 3221, 3253
Alw21I GWGCWC 5 cut(s) 906, 1676, 2180, 2637, 4883
Alw26I GTCTC 8 cut(s) 1359, 1674, 4238, 4905, 4983, 4995, 5022, 5278
Ama87I CYCGRG 2 cut(s) 155, 867
Aor51HI AGCGCT 1 cut(s) 275
AseI ATTAAT 1 cut(s) 1323
AsiGI ACCGGT 1 cut(s) 22
Asp700I GAANNNNTTC 3 cut(s) 964, 1797, 3957
Asp718I GGTACC 1 cut(s) 1763
AspLEI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4841, 4922, 5039
AspS9I GGNCC 9 cut(s) 325, 1340, 1708, 2322, 4092, 4176, 4541, 4685, 5201
AsuC2I CCSGG 1 cut(s) 3941
AsuII TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5359
AvaI CYCGRG 2 cut(s) 155, 867
AvaII GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4685, 5201
BaeGI GKGCMC 3 cut(s) 1182, 4696, 5000
BaeI ACNNNNGTAYC 4 cut(s) 1747, 1780, 2742, 2775
BalI TGGCCA 2 cut(s) 1484, 1559
BamHI GGATCC 2 cut(s) 706, 2269
BanI GGYRCC 1 cut(s) 1763
BanII GRGCYC 3 cut(s) 906, 2180, 2637
BbsI GAAGAC 2 cut(s) 582, 4045
Bbv12I GWGCWC 5 cut(s) 906, 1676, 2180, 2637, 4883
BceAI ACGGC 3 cut(s) 4329, 4826, 4907
BcgI CGANNNNNNTGC 2 cut(s) 1769, 1803
BciT130I CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BciVI GTATCC 3 cut(s) 617, 1040, 2208
BclI TGATCA 3 cut(s) 753, 829, 3406
BcnI CCSGG 1 cut(s) 3941
BcoDI GTCTC 8 cut(s) 1359, 1674, 4238, 4905, 4983, 4995, 5022, 5278
BcuI ACTAGT 3 cut(s) 758, 4184, 4418
BfmI CTRYAG 7 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819
BfoI RGCGCY 3 cut(s) 277, 3424, 4842
BfuAI ACCTGC 1 cut(s) 5083
BfuI GTATCC 3 cut(s) 617, 1040, 2208
BglI GCCNNNNNGGC 1 cut(s) 4313
BmcAI AGTACT 1 cut(s) 4291
Bme18I GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4685, 5201
BmeT110I CYCGRG 2 cut(s) 155, 867
BmgT120I GGNCC 9 cut(s) 325, 1340, 1708, 2322, 4092, 4176, 4541, 4685, 5201
BmrI ACTGGG 4 cut(s) 1005, 1384, 3113, 4027
BmuI ACTGGG 4 cut(s) 1005, 1384, 3113, 4027
BpiI GAAGAC 2 cut(s) 582, 4045
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 4 cut(s) 1698, 2009, 3278, 3688
Bpu14I TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5359
BpuEI CTTGAG 3 cut(s) 600, 2311, 4938
BpuMI CCSGG 1 cut(s) 3941
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 7 cut(s) 361, 834, 1230, 2948, 3031, 5394, 5412
BsaHI GRCGYC 1 cut(s) 1093
BsaI GGTCTC 1 cut(s) 4238
BsaJI CCNNGG 9 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4238, 4356, 4688
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 4 cut(s) 4405, 4435, 5185, 5215
Bse118I RCCGGY 3 cut(s) 22, 2121, 2492
Bse3DI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4336
Bse8I GATNNNNATC 7 cut(s) 361, 834, 1230, 2948, 3031, 5394, 5412
BseBI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 9 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4238, 4356, 4688
BseJI GATNNNNATC 7 cut(s) 361, 834, 1230, 2948, 3031, 5394, 5412
BseMI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4336
BseRI GAGGAG 2 cut(s) 1079, 5327
BseSI GKGCMC 3 cut(s) 1182, 4696, 5000
BseYI CCCAGC 5 cut(s) 3975, 4278, 4383, 4568, 5210
BsgI GTGCAG 4 cut(s) 2443, 3395, 5127, 5250
Bsh1236I CGCG 3 cut(s) 401, 3428, 3552
BshNI GGYRCC 1 cut(s) 1763
BshTI ACCGGT 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 5 cut(s) 906, 1676, 2180, 2637, 4883
BsiHKCI CYCGRG 2 cut(s) 155, 867
BsiSI CCGG 5 cut(s) 23, 2122, 2493, 3854, 3941
BsmAI GTCTC 8 cut(s) 1359, 1674, 4238, 4905, 4983, 4995, 5022, 5278
BsmI GAATGC 4 cut(s) 1371, 3796, 4531, 4570
Bso31I GGTCTC 1 cut(s) 4238
BsoBI CYCGRG 2 cut(s) 155, 867
Bsp119I TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5359
Bsp1286I GDGCHC 8 cut(s) 906, 1182, 1676, 2180, 2637, 4696, 4883, 5000
Bsp1407I TGTACA 2 cut(s) 83, 1078
Bsp19I CCATGG 1 cut(s) 4238
Bsp68I TCGCGA 1 cut(s) 3428
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 3 cut(s) 401, 3428, 3552
BspHI TCATGA 1 cut(s) 73
BspMAI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
BspMI ACCTGC 1 cut(s) 5083
BspT104I TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5359
BspT107I GGYRCC 1 cut(s) 1763
BspTNI GGTCTC 1 cut(s) 4238
BsrDI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4336
BsrFI RCCGGY 3 cut(s) 22, 2121, 2492
BsrGI TGTACA 2 cut(s) 83, 1078
BssAI RCCGGY 3 cut(s) 22, 2121, 2492
BssECI CCNNGG 9 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4238, 4356, 4688
BssNI GRCGYC 1 cut(s) 1093
BssT1I CCWWGG 6 cut(s) 897, 2116, 4095, 4238, 4356, 4688
Bst2UI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
Bst6I CTCTTC 4 cut(s) 982, 2584, 3744, 5216
BstACI GRCGYC 1 cut(s) 1093
BstAPI GCANNNNNTGC 4 cut(s) 1122, 1642, 1658, 1828
BstAUI TGTACA 2 cut(s) 83, 1078
BstBI TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5359
BstC8I GCNNGC 9 cut(s) 278, 427, 1466, 1659, 2123, 2790, 4668, 4782, 4860
BstDSI CCRYGG 1 cut(s) 4238
BstFNI CGCG 3 cut(s) 401, 3428, 3552
BstH2I RGCGCY 3 cut(s) 277, 3424, 4842
BstHHI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4841, 4922, 5039
BstMAI GTCTC 8 cut(s) 1359, 1674, 4238, 4905, 4983, 4995, 5022, 5278
BstNI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BstNSI RCATGY 5 cut(s) 2705, 3501, 3686, 4346, 5324
BstSFI CTRYAG 7 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819
BstSLI GKGCMC 3 cut(s) 1182, 4696, 5000
BstUI CGCG 3 cut(s) 401, 3428, 3552
BstV2I GAAGAC 2 cut(s) 582, 4045
BstX2I RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
BstXI CCANNNNNNTGG 3 cut(s) 4125, 4170, 4209
BstYI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
Bsu15I ATCGAT 1 cut(s) 92
BsuI GTATCC 3 cut(s) 617, 1040, 2208
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 4238
BtgZI GCGATG 1 cut(s) 1152
BtsI GCAGTG 1 cut(s) 3993
BtuMI TCGCGA 1 cut(s) 3428
BveI ACCTGC 1 cut(s) 5083
Cac8I GCNNGC 9 cut(s) 278, 427, 1466, 1659, 2123, 2790, 4668, 4782, 4860
CciI TCATGA 1 cut(s) 73
CfoI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4841, 4922, 5039
Cfr10I RCCGGY 3 cut(s) 22, 2121, 2492
Cfr13I GGNCC 9 cut(s) 325, 1340, 1708, 2322, 4092, 4176, 4541, 4685, 5201
ClaI ATCGAT 1 cut(s) 92
CseI GACGC 1 cut(s) 1082
CspAI ACCGGT 1 cut(s) 22
CspCI CAANNNNNGTGG 4 cut(s) 2463, 2498, 3511, 3546
DraI TTTAAA 1 cut(s) 2965
DraIII CACNNNGTG 1 cut(s) 1349
DrdI GACNNNNNNGTC 1 cut(s) 1313
DseDI GACNNNNNNGTC 1 cut(s) 1313
EaeI YGGCCR 4 cut(s) 348, 1482, 1557, 2119
Eam1104I CTCTTC 4 cut(s) 982, 2584, 3744, 5216
EarI CTCTTC 4 cut(s) 982, 2584, 3744, 5216
Ecl136II GAGCTC 3 cut(s) 904, 2178, 2635
Eco130I CCWWGG 6 cut(s) 897, 2116, 4095, 4238, 4356, 4688
Eco147I AGGCCT 2 cut(s) 1195, 1252
Eco24I GRGCYC 3 cut(s) 906, 2180, 2637
Eco31I GGTCTC 1 cut(s) 4238
Eco47I GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4685, 5201
Eco47III AGCGCT 1 cut(s) 275
Eco53kI GAGCTC 3 cut(s) 904, 2178, 2635
Eco57I CTGAAG 7 cut(s) 290, 317, 594, 1812, 3248, 4737, 4935
Eco88I CYCGRG 2 cut(s) 155, 867
EcoICRI GAGCTC 3 cut(s) 904, 2178, 2635
EcoO109I RGGNCCY 3 cut(s) 325, 1708, 4685
EcoRI GAATTC 2 cut(s) 2231, 3935
EcoRII CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
EcoT14I CCWWGG 6 cut(s) 897, 2116, 4095, 4238, 4356, 4688
EcoT22I ATGCAT 3 cut(s) 1006, 3292, 5373
EcoT38I GRGCYC 3 cut(s) 906, 2180, 2637
ErhI CCWWGG 6 cut(s) 897, 2116, 4095, 4238, 4356, 4688
FalI AAGNNNNNCTT 6 cut(s) 915, 947, 2727, 2759, 3456, 3488
FauI CCCGC 3 cut(s) 989, 2788, 4675
FauNDI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
FbaI TGATCA 3 cut(s) 753, 829, 3406
FblI GTMKAC 1 cut(s) 4634
FriOI GRGCYC 3 cut(s) 906, 2180, 2637
GlaI GCGC 8 cut(s) 275, 1548, 3422, 3502, 3553, 4840, 4921, 5038
GsaI CCCAGC 5 cut(s) 3979, 4282, 4387, 4572, 5214
GsuI CTGGAG 4 cut(s) 1698, 2009, 3278, 3688
HaeII RGCGCY 3 cut(s) 277, 3424, 4842
HapII CCGG 5 cut(s) 23, 2122, 2493, 3854, 3941
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4841, 4922, 5039
Hin1I GRCGYC 1 cut(s) 1093
Hin6I GCGC 8 cut(s) 274, 1547, 3421, 3501, 3552, 4839, 4920, 5037
HinP1I GCGC 8 cut(s) 274, 1547, 3421, 3501, 3552, 4839, 4920, 5037
HpaII CCGG 5 cut(s) 23, 2122, 2493, 3854, 3941
Hpy99I CGWCG 3 cut(s) 2649, 3166, 4315
HpyCH4IV ACGT 2 cut(s) 1296, 5176
HpySE526I ACGT 2 cut(s) 1296, 5176
Hsp92I GRCGYC 1 cut(s) 1093
HspAI GCGC 8 cut(s) 274, 1547, 3421, 3501, 3552, 4839, 4920, 5037
KpnI GGTACC 1 cut(s) 1767
KroI GCCGGC 1 cut(s) 2121
KroNI GCCGGC 1 cut(s) 2123
Ksp22I TGATCA 3 cut(s) 753, 829, 3406
LmnI GCTCC 8 cut(s) 110, 434, 901, 1420, 3707, 3830, 4849, 5314
MaeII ACGT 2 cut(s) 1296, 5176
MaeIII GTNAC 7 cut(s) 688, 892, 1392, 2095, 2853, 2954, 3882
MfeI CAATTG 3 cut(s) 437, 822, 1553
MflI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
MhlI GDGCHC 8 cut(s) 906, 1182, 1676, 2180, 2637, 4696, 4883, 5000
MlsI TGGCCA 2 cut(s) 1484, 1559
MluNI TGGCCA 2 cut(s) 1484, 1559
MlyI GAGTC 4 cut(s) 1904, 2060, 2344, 2807
MmeI TCCRAC 8 cut(s) 25, 156, 1280, 2247, 3060, 4015, 4502, 4599
Mox20I TGGCCA 2 cut(s) 1484, 1559
Mph1103I ATGCAT 3 cut(s) 1006, 3292, 5373
MroNI GCCGGC 1 cut(s) 2121
MroXI GAANNNNTTC 3 cut(s) 964, 1797, 3957
MscI TGGCCA 2 cut(s) 1484, 1559
MslI CAYNNNNRTG 7 cut(s) 261, 2841, 4084, 4123, 4168, 4207, 4237
Msp20I TGGCCA 2 cut(s) 1484, 1559
MspA1I CMGCKG 4 cut(s) 1444, 3434, 3623, 4670
MspI CCGG 5 cut(s) 23, 2122, 2493, 3854, 3941
MunI CAATTG 3 cut(s) 437, 822, 1553
Mva1269I GAATGC 4 cut(s) 1371, 3796, 4531, 4570
MvaI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
MvnI CGCG 3 cut(s) 401, 3428, 3552
NaeI GCCGGC 1 cut(s) 2123
NciI CCSGG 1 cut(s) 3941
NcoI CCATGG 1 cut(s) 4238
NdeI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
NgoMIV GCCGGC 1 cut(s) 2121
NmuCI GTSAC 4 cut(s) 892, 1392, 2954, 3882
NruI TCGCGA 1 cut(s) 3428
NsiI ATGCAT 3 cut(s) 1006, 3292, 5373
NspI RCATGY 5 cut(s) 2705, 3501, 3686, 4346, 5324
NspV TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5359
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 1 cut(s) 73
PceI AGGCCT 2 cut(s) 1195, 1252
PciI ACATGT 3 cut(s) 3682, 4342, 5320
PctI GAATGC 4 cut(s) 1371, 3796, 4531, 4570
PdiI GCCGGC 1 cut(s) 2123
PdmI GAANNNNTTC 3 cut(s) 964, 1797, 3957
PflMI CCANNNNNTGG 4 cut(s) 143, 470, 4521, 5210
PfoI TCCNGGA 2 cut(s) 3939, 3961
PinAI ACCGGT 1 cut(s) 22
PleI GAGTC 4 cut(s) 1903, 2060, 2344, 2807
PpsI GAGTC 4 cut(s) 1903, 2060, 2344, 2807
PpuMI RGGWCCY 3 cut(s) 325, 1708, 4685
PscI ACATGT 3 cut(s) 3682, 4342, 5320
PshBI ATTAAT 1 cut(s) 1323
PsiI TTATAA 1 cut(s) 3537
Psp124BI GAGCTC 3 cut(s) 906, 2180, 2637
Psp5II RGGWCCY 3 cut(s) 325, 1708, 4685
Psp6I CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
PspFI CCCAGC 5 cut(s) 3975, 4278, 4383, 4568, 5210
PspGI CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
PspPI GGNCC 9 cut(s) 325, 1340, 1708, 2322, 4092, 4176, 4541, 4685, 5201
PspPPI RGGWCCY 3 cut(s) 325, 1708, 4685
PstI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
PsuI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
PvuII CAGCTG 3 cut(s) 1444, 3434, 3623
RruI TCGCGA 1 cut(s) 3428
RseI CAYNNNNRTG 7 cut(s) 261, 2841, 4084, 4123, 4168, 4207, 4237
SacI GAGCTC 3 cut(s) 906, 2180, 2637
Sau96I GGNCC 9 cut(s) 325, 1340, 1708, 2322, 4092, 4176, 4541, 4685, 5201
ScaI AGTACT 1 cut(s) 4291
SchI GAGTC 4 cut(s) 1904, 2060, 2344, 2807
SduI GDGCHC 8 cut(s) 906, 1182, 1676, 2180, 2637, 4696, 4883, 5000
SfcI CTRYAG 7 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819
Sfr274I CTCGAG 1 cut(s) 155
SfuI TTCGAA 5 cut(s) 2002, 2024, 3711, 3951, 5359
SinI GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4685, 5201
SlaI CTCGAG 1 cut(s) 155
SmiMI CAYNNNNRTG 7 cut(s) 261, 2841, 4084, 4123, 4168, 4207, 4237
SmlI CTYRAG 4 cut(s) 155, 615, 2326, 4953
SmoI CTYRAG 4 cut(s) 155, 615, 2326, 4953
SpeI ACTAGT 3 cut(s) 758, 4184, 4418
SseBI AGGCCT 2 cut(s) 1195, 1252
SspI AATATT 2 cut(s) 1087, 1526
SstI GAGCTC 3 cut(s) 906, 2180, 2637
StuI AGGCCT 2 cut(s) 1195, 1252
StyI CCWWGG 6 cut(s) 897, 2116, 4095, 4238, 4356, 4688
TaiI ACGT 2 cut(s) 1299, 5179
TaqII GACCGA 1 cut(s) 2310
TatI WGTACW 7 cut(s) 83, 1078, 3899, 4103, 4187, 4289, 4421
TauI GCSGC 2 cut(s) 2708, 4748
TseFI GTSAC 4 cut(s) 892, 1392, 2954, 3882
Tsp45I GTSAC 4 cut(s) 892, 1392, 2954, 3882
TspGWI ACGGA 1 cut(s) 636
Van91I CCANNNNNTGG 4 cut(s) 143, 470, 4521, 5210
VpaK11BI GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4685, 5201
VspI ATTAAT 1 cut(s) 1323
XceI RCATGY 5 cut(s) 2705, 3501, 3686, 4346, 5324
XcmI CCANNNNNNNNNTGG 3 cut(s) 589, 1477, 4407
XhoI CTCGAG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 4634
XmnI GAANNNNTTC 3 cut(s) 964, 1797, 3957
ZrmI AGTACT 1 cut(s) 4291
Zsp2I ATGCAT 3 cut(s) 1006, 3292, 5373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.