RLG00000030220

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006658
Physical Location & Seq
Reverse (-)
57825940 .. 57840228
14289 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030220

Sequence Viewer

Length: 5820 bp
ATGTGGTTGTGTATTTATGGTCTCAGAGTATCTAAGGAGAGGTTGAGAGAAATGGAGGAAGTGTCTTGTTCACCGGTTTTAGATGGGGAGTTAGTTGGAATTAAATTTGGTTTGGCAACTCATCATGAAATTTGTACAGCATCGATGATGGGATGCTCTATTAGCCATGCCAGTCAGCTTTCCAACCCATTTCTTGGCCTTCCTCTCGAGTTTGGGAAGTGTGAATCTTGTGGCACTTCCGAACCTGGAAAGTGCGAAGGGCATTTTGGATATATCGACTTACCATTACCGATATATCATCCTAACCACGTCAGTGAGCTGAAGCGCTTGCTGAGTTTTTTGTGCCTGAAGTGCTTAAAAATGAAAAAGAACAAGGTCCCAAGCAAGAGTGCTGGTTTGGCCGAAAGATTGTTATCTGTATGTTGTGAGGATGCTTCACAAGTTTCTATCACAGAGATTAAACCTACAGATGGGGCTTGCTCCTTGCAATTGAAACTACCTTCTAATAAAAAACCACCGCCTGGTTTTTGGAATTTCCTAGAAAGATATGGTTTTCGTTATGGTGAGGGTGTGAAACGCACTTTGCTTCCTAGAGAGGTGATGCAGATTCTCAAAAGAATCCCTGAAGACACCAGAAGAAAACTGGCTGCTAAAGGCTATAATCCTCAAGATGGATACATCTTGAATCATATACCTGTCCCTCCTAATTGCTTGTCTGTGCCTGAAATATCTGATGGTGTTACTGTTATGTCTGCGGATCCGTCAATATCAATGCTCAAAAAAGTTCTAAGGCAAGTTGAAATGATCACTAGTTCAAGGTCTGGTACAGCAAATTTTGAATCTGAAATTGTTGAGGCGAATGAGTTACAAGCAATTGTTGATCAGTATCTTCAAGTTAGGGGTACTGGAAAGGCATCCCGAGATATTGATGCAAGATTTGGAGTGACCAAGGAGCTCAACGATTCTTCAACAAAAGCATGGCTTGAAAAGATGAGGACATTGTTCATCAGAAAGGGTTCAGGCTTCTCTTCCCGCACAGTAATTACTGGGGATGCATATAGAAGGGTGAATGAAGTTGGTATCCCATATGAAATTGCTCAAAGAATAACGTTTGAGGAGAAGGTCAATGTACATAATATTAGGCGTCTGCAAGAGCTGGTAGATAGCAAGTTATGCCTAACCTACAGTGATGGTTCATCAACTTACTCTTTAAGGGAAGGTTCAAAGGGGCACACATTTCTGAGGCCTGGTCAAGTGGTGCATAGGAGGATAATGGATGGGGATCTTGTTTTCGTAAATAGGCCTCCAACTACCCATAAACATTCGCTTCAAGCACTTCAAGTTTACGTCCATGATGACCATGTGGTCAAGATTAATCCTCTAATATGTGGACCACTAAGTGCTGACTTTGATGGAGACTGCATTCATTTGTTCTATCCCCAGTCACTTGCGGCAAAGGCAGAAGTTATGGAGCTTTTTTCAGTGGAGAAACAGCTGCTTAGTTCTCACAGTGGCAAGCCCAACTTGCAACTGGCCACCGATTCACTTTTGTCATTAAAAATGATGTTTAAGAAATATTTTTTTGACAAAGCAGCAGCGCAGCAATTGGCCATGTTTGTTTCATCTTCATTGCCACAACCAGCTTTATTGAAAGCTAATTCTGCAGTTCCATATTGGACTGCTTTGCAGATATTGCAGACTGCATTGCCTGCTCATTTTGAGTGCTCTGGAGACAGGCACTTGGTCAGTGCTAGTGAGGTCCTCAAACTTGATTGTAATACAAGTCTTATACCAGCTCTTATCAATGACATTGGTACCTCTATTTTCTTCGAGAAAAGTGCTGAAGATGTTCTCAATTTCTTTAATTCTATGCAGCCATTGCTGATGGAGAATTTATTTTCAGAAGGGTTCAGTGTAGGTTTGGAGGATTTTGCCATGTCTAGGGAGTCCATTCAGGAGATTAGAAAGGGACTTAAAATCATTTCTCCTTTGCTGTATCATATGAGGGCAACATATAATGAGCTTGTTGAGTTGCAACTGGAGAATCACATTCGAACGGTGAAACAACCAGTTTCGAATTTTATATTAAATTCATCGGCATTGGGTGATTTAATTGACTCTAAGAGTGATTCAGCCATGAACAAAGTAACTCAACAAGTTGGGTTCCTTGGCCGGCAACTTTATGACCAGGGGAAACTCTACTCAAAAACATTGGTTGAGGATGTGAGCTTTCTGTATCAGAGCAAGTATCCATCTGGCATCGTGGATTATCCCTCTGCAGAATTCGGATTAGTTCATAGTGGTTTTGTTCATGGGTTGGATCCTGTTGAGGGAATGATTCATTCCATTGCTACAAGGGAAGTAATTGTTCGGTCCTCAAGAGGGTTGTCTGAACCTGGGACTCTATTCAAAAATCTCATGGCTACTCTCCGAGATGTTGTCATTTGCTATGATGGCACAGTGAGGAATGTGTGCAGTAATTCTATAATTCAGTTCGAGTATGGAGTAAAGAGCGGATCTAGTCCACAAAATTTGTTCCCTGCCGGTGAACCTGTGGGTGTGTTAGCTGCAACAGCAATGTCAAATCCAGCATACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCCTCATGGGATCTGATGAAGGAAATACTGCTCTGCAAAGTCAGTTTCAAGAACGAGCTCATTGATCGTCGTGTAATCTTGTATCTGAATGACTGTGGTTGCGGAAGAAACTACTGCAGAGAACATGCGGCATATTTGGTCAAGAATCAGTTGAAAAAAGTCAGCCTTAAAGATACTGCATTAGAGTTTATGATTGAATATCAAAATCAGCGAGCTGCGGGAAGCATGGAAATTGACTCTGGCCTTGTTGGTCATGTTCATTTGAATGAGATGCTGTTACGGGAGTTAAATCTGGGAATGTATGAGATCCTTCAAAAATGCGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTTGGGAAGAAGATGAACATCGGTGACATTTTTAAAAGGACAGTTTTATCGTACAGTGAATGTTGTTCTTTTCATCAATCCTCTGCTGACAATAGTTCTGGATCTCCTTGCTTGATGTTCTTCTACCAAGATTTTAATAATTCAGAGTTGGAGGCCATTTCACAAATCTTGGCTGACTTTATTTGCCCGGTTCTTCTAAAAACAGTAATTAAAGGTGATCCTCGAATTTCGTCGGCGAACATAATTTGGATCAACTCTGAATCAACAACTTGGATAAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCATGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATGTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAAGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCGACAGCTGTTGCAATGGTGGCAAAAGGTGTTCTCAAAGAGCATCTTATTCTCTTGGCAAACAGTATGACATGCGCTGGAAATTTCGTTGGCTTCAATCCTGGTGGTTATAAAGCACTATCACGCGCGCTGAATATACAAGTACCCTTTACAGAAGCAACATTGTTTACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCTTTGTCGAGCATAGTTGCATCATGTTCCTGGGGTAAACATGTAGCTGTTGGTACTGGCTCCAGATTCGAAATCCTCTGGGACACAAGAGAGGGAGGTTTGAACGAAGAGGGTGGGATAGATGTTTTCAACTTTCTACATATGGTGAATGCTGCAAATGGAGAAGAATCAACTACTGCAGCTTTGGGAGCAGAAATTGATGATCTTGTGCCGGAATATGAAAATGGAGATGTGTCCTTGTCACCAGAACATAACTGTACTTCTGATAAGCCAGTGTTTGAAGATATTGTTGAATTCCCCGATAGTTTCGAAAATGTTCCTGGAAAATCTAGCTGGGATTCATCTCTCAACACCGCTTCTTCTGGTGGGAAAGACTGGGGGGCAGTTAAAGTTGGAAGACAAGATAGCATTTCAGCAGAACCCCAAGTGGATAGCACATCTGCATGGGGGGCCAAGGCTCGTACAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCTCATCTGCATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGCACAGCCAAGACTGCTACATCTCCATGGGAGACCAACACTGGTAGAGAAGATTCAACATCGGGCTGGGGGACAAGTACTGCCAGAGAAGATGCCACGATGGCATGGGGAAAAAGCAATGAAAAGAAACATGTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTTGCTGGGGGACAGAAAATCCACAATCTACATGGAGGACTAGTACAGAAAGCGAGAATGGTTGGTCTGGTGGTGGGGTGAGTAAAGCTGAATCAACAGATGTCCAGTCCAAGAAAGCACAAGAAGATTCTACATCCAATGGTTGGAATGCCTTTTCTGGTGGGCCAACAAAACCCCAAAAGGAGAATGCTGGGTCTGGATGGGGCATGAAAGGTTTTGAGAAGAAAGATGGTAATGAGTTGGAACAAGATAAATCTACTGCCCGTCATTCTTGGGGGCAGCAAAATGCCACTACTTCAGAAGGTTCTTGGGGTCAGGAAACGCCTGCCGATACTTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCTGAGATATCTAAAGGCGCTTGGGGGGAGCAAAAGCCTGCTGATGCTAATTCTCAGGGCACTTGGGGACGGCAAAAGTCTCCTGAGATATCTAAAGGCGCTTGGGGGGAGCAAAAGCCTGCTGATGCTAATTCTCAGGGCACTTGGGGACGGCAAAAGTCTCCTGATATTTCTGAAGGCGCATGGGGGCAGCAAAATCCTGATACAACTTCTCAAGGCTCTTGGGGACAGCAAAAGTCTCCTGAGATGTCTCAGGGCACTTGGGGAAGGCAAAAGTCTCCTGATATTTCTCAAGGCGCATGGGGGCAACAAAAGCCTTCTGATACTTCTCAACCTGCAACTGTGAATCAATGGGATACTCCAAGTGCAGGAGATGGAAGTGCCGGTGAAAGACAGCAACAATGGGGTCATAATGGAGATGCGAACAAGAAAAGACGTTTTGAAGGTGGCAGGAGTTGGGGTCCAAATGCTGGGGAGTGGAAGAGTAAGAATAGTCGCCCTGCTAATGCTGATTCTAGTGTGGCTGCACTATATACCGCAACGAGACAAAGATTGGATATTTTCACTTCTGAGGAGCAACATGTTCTTTCCCATATTGAGCCGATTATGCTGTCTATTCGAAGAATAATGCATCAATCTGGCTACAATGATGGAGATCGGTTGTCAGATGAGGATCGATCATACATACTTGAAAATGTGTTCACACACCACCCAGATAAAGATGTTAAGATGGGTTCTGGAATTGACTATTTTACAGTTGATAGGCATGGAAGTTTTCAAGAAAGCCGATGCCTTTTTGTCATATCAACTGATGGTCGCAAGGAGGACTTTTCCTACAGAAAATGCCTGCAAAATATGATAAAGGGAAAGTATCCTGAGCTAGCTGAAGCATTCAATGACAAGTACTTCAGCAAACCTCGGCGGAATCCCATGTTCGGTCAGACCCCTCCAGAGAGAACTGGGAATGAGGAGACACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1940

Amino Acids

213.92

Weight (kDa)

6.15

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 65 - 298 1.1e-10 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 341 - 495 5.2e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 498 - 643 6.5e-10 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 772 - 1198 9.6e-10 RNA polymerase Rpb1, domain 5
DUF3223 PF11523 1821 - 1896 5e-27 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3588
AasI GACNNNNNNGTC 1 cut(s) 1364
Acc36I ACCTGC 1 cut(s) 5182
Acc65I GGTACC 1 cut(s) 1814
AccB1I GGYRCC 1 cut(s) 1814
AccB7I CCANNNNNTGG 4 cut(s) 194, 521, 4617, 5309
AccBSI CCGCTC 1 cut(s) 2514
AccII CGCG 3 cut(s) 3479, 3603, 3605
AclI AACGTT 1 cut(s) 1109
AcoI YGGCCR 4 cut(s) 399, 1533, 1608, 2170
AcuI CTGAAG 9 cut(s) 341, 368, 645, 1863, 3299, 4755, 5034, 5731, 5745
AcyI GRCGYC 1 cut(s) 1144
AdeI CACNNNGTG 1 cut(s) 1400
AfeI AGCGCT 1 cut(s) 326
AflIII ACRYGT 3 cut(s) 3733, 4438, 5419
AgeI ACCGGT 1 cut(s) 73
AhlI ACTAGT 3 cut(s) 809, 4280, 4514
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 9 cut(s) 244, 520, 1246, 2187, 2395, 3392, 3577, 3722, 4012
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 3272, 3304
AleI CACNNNNGTG 1 cut(s) 312
Alw21I GWGCWC 3 cut(s) 957, 1727, 2688
Ama87I CYCGRG 2 cut(s) 206, 918
Aor51HI AGCGCT 1 cut(s) 326
AseI ATTAAT 1 cut(s) 1374
AsiGI ACCGGT 1 cut(s) 73
Asp700I GAANNNNTTC 3 cut(s) 1015, 1848, 4008
Asp718I GGTACC 1 cut(s) 1814
AspS9I GGNCC 8 cut(s) 376, 1391, 1759, 2373, 4143, 4272, 4637, 5300
AsuC2I CCSGG 1 cut(s) 3170
AsuII TTCGAA 5 cut(s) 2053, 2075, 3762, 4002, 5458
AsuNHI GCTAGC 1 cut(s) 5719
AvaI CYCGRG 2 cut(s) 206, 918
AvaII GGWCC 6 cut(s) 376, 1391, 1759, 2373, 4272, 5300
BaeGI GKGCMC 5 cut(s) 1233, 4313, 4901, 4982, 5099
BaeI ACNNNNGTAYC 2 cut(s) 1798, 1831
BalI TGGCCA 2 cut(s) 1535, 1610
BamHI GGATCC 2 cut(s) 757, 2320
BanI GGYRCC 1 cut(s) 1814
BanII GRGCYC 2 cut(s) 957, 2688
BbsI GAAGAC 2 cut(s) 633, 4096
Bbv12I GWGCWC 3 cut(s) 957, 1727, 2688
BceAI ACGGC 3 cut(s) 4844, 4925, 5006
BcgI CGANNNNNNTGC 2 cut(s) 1820, 1854
BciT130I CCWGG 9 cut(s) 246, 522, 1248, 2189, 2397, 3394, 3579, 3724, 4014
BciVI GTATCC 5 cut(s) 668, 1091, 2259, 5188, 5721
BclI TGATCA 3 cut(s) 804, 880, 3457
BcnI CCSGG 1 cut(s) 3170
BcuI ACTAGT 3 cut(s) 809, 4280, 4514
BfmI CTRYAG 7 cut(s) 465, 1183, 1662, 2277, 2743, 3870, 5674
BfoI RGCGCY 4 cut(s) 328, 3475, 4860, 4941
BfuAI ACCTGC 1 cut(s) 5182
BfuI GTATCC 5 cut(s) 668, 1091, 2259, 5188, 5721
BglI GCCNNNNNGGC 1 cut(s) 4409
BmcAI AGTACT 2 cut(s) 4387, 5744
Bme18I GGWCC 6 cut(s) 376, 1391, 1759, 2373, 4272, 5300
BmeT110I CYCGRG 2 cut(s) 206, 918
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 8 cut(s) 376, 1391, 1759, 2373, 4143, 4272, 4637, 5300
BmiI GGNNCC 9 cut(s) 378, 759, 1816, 2165, 2322, 3754, 4144, 4273, 5301
BmrI ACTGGG 4 cut(s) 1056, 1435, 4078, 5808
BmtI GCTAGC 1 cut(s) 5723
BmuI ACTGGG 4 cut(s) 1056, 1435, 4078, 5808
BpiI GAAGAC 2 cut(s) 633, 4096
BpmI CTGGAG 5 cut(s) 1749, 2060, 3329, 3739, 5772
Bpu10I CCTNAGC 1 cut(s) 5715
Bpu14I TTCGAA 5 cut(s) 2053, 2075, 3762, 4002, 5458
BpuEI CTTGAG 4 cut(s) 651, 2362, 5037, 5115
BpuMI CCSGG 1 cut(s) 3170
Bsa29I ATCGAT 2 cut(s) 143, 5515
BsaBI GATNNNNATC 6 cut(s) 412, 885, 1281, 2999, 5493, 5511
BsaHI GRCGYC 1 cut(s) 1144
BsaI GGTCTC 2 cut(s) 26, 4334
BsaJI CCNNGG 9 cut(s) 948, 2167, 2188, 2396, 3723, 4146, 4334, 4452, 5756
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 7 cut(s) 47, 77, 4501, 4531, 5284, 5314, 5801
Bse118I RCCGGY 4 cut(s) 73, 2172, 2543, 5222
Bse3DI GCAATG 7 cut(s) 1628, 1703, 1877, 2346, 2583, 3498, 4432
Bse8I GATNNNNATC 6 cut(s) 412, 885, 1281, 2999, 5493, 5511
BseBI CCWGG 9 cut(s) 246, 522, 1248, 2189, 2397, 3394, 3579, 3724, 4014
BseCI ATCGAT 2 cut(s) 143, 5515
BseDI CCNNGG 9 cut(s) 948, 2167, 2188, 2396, 3723, 4146, 4334, 4452, 5756
BseJI GATNNNNATC 6 cut(s) 412, 885, 1281, 2999, 5493, 5511
BseMI GCAATG 7 cut(s) 1628, 1703, 1877, 2346, 2583, 3498, 4432
BsePI GCGCGC 1 cut(s) 3603
BseRI GAGGAG 3 cut(s) 1130, 2620, 5426
BseSI GKGCMC 5 cut(s) 1233, 4313, 4901, 4982, 5099
BseYI CCCAGC 5 cut(s) 4026, 4374, 4479, 4664, 5309
BsgI GTGCAG 4 cut(s) 2494, 3446, 5226, 5349
Bsh1236I CGCG 3 cut(s) 3479, 3603, 3605
BshNI GGYRCC 1 cut(s) 1814
BshTI ACCGGT 1 cut(s) 73
BshVI ATCGAT 2 cut(s) 143, 5515
BsiHKAI GWGCWC 3 cut(s) 957, 1727, 2688
BsiHKCI CYCGRG 2 cut(s) 206, 918
BsiSI CCGG 6 cut(s) 74, 2173, 2544, 3170, 3905, 5223
BsmI GAATGC 5 cut(s) 1422, 3847, 4627, 4666, 5729
Bso31I GGTCTC 2 cut(s) 26, 4334
BsoBI CYCGRG 2 cut(s) 206, 918
Bsp119I TTCGAA 5 cut(s) 2053, 2075, 3762, 4002, 5458
Bsp1286I GDGCHC 8 cut(s) 957, 1233, 1727, 2688, 4313, 4901, 4982, 5099
Bsp1407I TGTACA 2 cut(s) 134, 1129
Bsp19I CCATGG 1 cut(s) 4334
Bsp68I TCGCGA 1 cut(s) 3479
BspDI ATCGAT 2 cut(s) 143, 5515
BspFNI CGCG 3 cut(s) 3479, 3603, 3605
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 9 cut(s) 378, 759, 1816, 2165, 2322, 3754, 4144, 4273, 5301
BspMAI CTGCAG 4 cut(s) 1666, 2281, 2747, 3874
BspMI ACCTGC 1 cut(s) 5182
BspOI GCTAGC 1 cut(s) 5723
BspT104I TTCGAA 5 cut(s) 2053, 2075, 3762, 4002, 5458
BspT107I GGYRCC 1 cut(s) 1814
BspTNI GGTCTC 2 cut(s) 26, 4334
BsrBI CCGCTC 1 cut(s) 2514
BsrDI GCAATG 7 cut(s) 1628, 1703, 1877, 2346, 2583, 3498, 4432
BsrFI RCCGGY 4 cut(s) 73, 2172, 2543, 5222
BsrGI TGTACA 2 cut(s) 134, 1129
BssAI RCCGGY 4 cut(s) 73, 2172, 2543, 5222
BssECI CCNNGG 9 cut(s) 948, 2167, 2188, 2396, 3723, 4146, 4334, 4452, 5756
BssHII GCGCGC 1 cut(s) 3603
BssNI GRCGYC 1 cut(s) 1144
BssT1I CCWWGG 5 cut(s) 948, 2167, 4146, 4334, 4452
Bst2UI CCWGG 9 cut(s) 246, 522, 1248, 2189, 2397, 3394, 3579, 3724, 4014
Bst6I CTCTTC 3 cut(s) 1033, 3795, 5315
BstACI GRCGYC 1 cut(s) 1144
BstAPI GCANNNNNTGC 4 cut(s) 1173, 1693, 1709, 1879
BstAUI TGTACA 2 cut(s) 134, 1129
BstBI TTCGAA 5 cut(s) 2053, 2075, 3762, 4002, 5458
BstDSI CCRYGG 1 cut(s) 4334
BstFNI CGCG 3 cut(s) 3479, 3603, 3605
BstH2I RGCGCY 4 cut(s) 328, 3475, 4860, 4941
BstNI CCWGG 9 cut(s) 246, 522, 1248, 2189, 2397, 3394, 3579, 3724, 4014
BstNSI RCATGY 5 cut(s) 2756, 3552, 3737, 4442, 5423
BstSFI CTRYAG 7 cut(s) 465, 1183, 1662, 2277, 2743, 3870, 5674
BstSLI GKGCMC 5 cut(s) 1233, 4313, 4901, 4982, 5099
BstUI CGCG 3 cut(s) 3479, 3603, 3605
BstV2I GAAGAC 2 cut(s) 633, 4096
BstX2I RGATCY 7 cut(s) 757, 1282, 2320, 2516, 2638, 2934, 3083
BstXI CCANNNNNNTGG 3 cut(s) 4176, 4266, 4305
BstYI RGATCY 7 cut(s) 757, 1282, 2320, 2516, 2638, 2934, 3083
Bsu15I ATCGAT 2 cut(s) 143, 5515
BsuI GTATCC 5 cut(s) 668, 1091, 2259, 5188, 5721
BsuTUI ATCGAT 2 cut(s) 143, 5515
BtgI CCRYGG 1 cut(s) 4334
BtrI CACGTC 1 cut(s) 310
BtuMI TCGCGA 1 cut(s) 3479
BveI ACCTGC 1 cut(s) 5182
CciI TCATGA 1 cut(s) 124
Cfr10I RCCGGY 4 cut(s) 73, 2172, 2543, 5222
Cfr13I GGNCC 8 cut(s) 376, 1391, 1759, 2373, 4143, 4272, 4637, 5300
ClaI ATCGAT 2 cut(s) 143, 5515
CseI GACGC 1 cut(s) 1133
CspAI ACCGGT 1 cut(s) 73
CspCI CAANNNNNGTGG 4 cut(s) 2514, 2549, 3562, 3597
DraI TTTAAA 1 cut(s) 3016
DraIII CACNNNGTG 1 cut(s) 1400
DrdI GACNNNNNNGTC 1 cut(s) 1364
DseDI GACNNNNNNGTC 1 cut(s) 1364
EaeI YGGCCR 4 cut(s) 399, 1533, 1608, 2170
Eam1104I CTCTTC 3 cut(s) 1033, 3795, 5315
EarI CTCTTC 3 cut(s) 1033, 3795, 5315
EciI GGCGGA 1 cut(s) 5776
Ecl136II GAGCTC 2 cut(s) 955, 2686
Eco130I CCWWGG 5 cut(s) 948, 2167, 4146, 4334, 4452
Eco147I AGGCCT 2 cut(s) 1246, 1303
Eco24I GRGCYC 2 cut(s) 957, 2688
Eco31I GGTCTC 2 cut(s) 26, 4334
Eco32I GATATC 2 cut(s) 4848, 4929
Eco47I GGWCC 6 cut(s) 376, 1391, 1759, 2373, 4272, 5300
Eco47III AGCGCT 1 cut(s) 326
Eco53kI GAGCTC 2 cut(s) 955, 2686
Eco57I CTGAAG 9 cut(s) 341, 368, 645, 1863, 3299, 4755, 5034, 5731, 5745
Eco88I CYCGRG 2 cut(s) 206, 918
EcoICRI GAGCTC 2 cut(s) 955, 2686
EcoO109I RGGNCCY 2 cut(s) 376, 1759
EcoRI GAATTC 2 cut(s) 2282, 3986
EcoRII CCWGG 9 cut(s) 244, 520, 1246, 2187, 2395, 3392, 3577, 3722, 4012
EcoRV GATATC 2 cut(s) 4848, 4929
EcoT14I CCWWGG 5 cut(s) 948, 2167, 4146, 4334, 4452
EcoT22I ATGCAT 3 cut(s) 1057, 3343, 5472
EcoT38I GRGCYC 2 cut(s) 957, 2688
ErhI CCWWGG 5 cut(s) 948, 2167, 4146, 4334, 4452
FauI CCCGC 2 cut(s) 1040, 2839
FauNDI CATATG 5 cut(s) 1087, 2001, 3409, 3687, 3834
FbaI TGATCA 3 cut(s) 804, 880, 3457
FriOI GRGCYC 2 cut(s) 957, 2688
GsaI CCCAGC 5 cut(s) 4030, 4378, 4483, 4668, 5313
GsuI CTGGAG 5 cut(s) 1749, 2060, 3329, 3739, 5772
HaeII RGCGCY 4 cut(s) 328, 3475, 4860, 4941
HapII CCGG 6 cut(s) 74, 2173, 2544, 3170, 3905, 5223
HgaI GACGC 1 cut(s) 1133
Hin1I GRCGYC 1 cut(s) 1144
HpaII CCGG 6 cut(s) 74, 2173, 2544, 3170, 3905, 5223
Hpy166II GTNNAC 8 cut(s) 71, 1345, 1391, 2525, 2549, 3645, 3731, 5541
Hpy8I GTNNAC 8 cut(s) 71, 1345, 1391, 2525, 2549, 3645, 3731, 5541
Hpy99I CGWCG 2 cut(s) 2700, 3217
HpyCH4IV ACGT 4 cut(s) 309, 1109, 1347, 5275
HpySE526I ACGT 4 cut(s) 309, 1109, 1347, 5275
Hsp92I GRCGYC 1 cut(s) 1144
KpnI GGTACC 1 cut(s) 1818
KroI GCCGGC 1 cut(s) 2172
KroNI GCCGGC 1 cut(s) 2174
Ksp22I TGATCA 3 cut(s) 804, 880, 3457
LmnI GCTCC 8 cut(s) 485, 952, 1471, 3758, 3881, 4867, 4948, 5413
MaeII ACGT 4 cut(s) 309, 1109, 1347, 5275
MaeIII GTNAC 8 cut(s) 739, 864, 943, 1443, 2146, 2904, 3005, 3933
MbiI CCGCTC 1 cut(s) 2514
MfeI CAATTG 3 cut(s) 488, 873, 1604
MflI RGATCY 7 cut(s) 757, 1282, 2320, 2516, 2638, 2934, 3083
MhlI GDGCHC 8 cut(s) 957, 1233, 1727, 2688, 4313, 4901, 4982, 5099
MlsI TGGCCA 2 cut(s) 1535, 1610
MluNI TGGCCA 2 cut(s) 1535, 1610
MlyI GAGTC 4 cut(s) 1955, 2111, 2395, 2858
MmeI TCCRAC 8 cut(s) 76, 207, 1331, 2298, 3111, 4066, 4598, 4695
Mox20I TGGCCA 2 cut(s) 1535, 1610
Mph1103I ATGCAT 3 cut(s) 1057, 3343, 5472
MroNI GCCGGC 1 cut(s) 2172
MroXI GAANNNNTTC 3 cut(s) 1015, 1848, 4008
MscI TGGCCA 2 cut(s) 1535, 1610
MslI CAYNNNNRTG 8 cut(s) 312, 2892, 4135, 4174, 4219, 4264, 4303, 4333
Msp20I TGGCCA 2 cut(s) 1535, 1610
MspA1I CMGCKG 3 cut(s) 1495, 3485, 3674
MspI CCGG 6 cut(s) 74, 2173, 2544, 3170, 3905, 5223
MunI CAATTG 3 cut(s) 488, 873, 1604
Mva1269I GAATGC 5 cut(s) 1422, 3847, 4627, 4666, 5729
MvaI CCWGG 9 cut(s) 246, 522, 1248, 2189, 2397, 3394, 3579, 3724, 4014
MvnI CGCG 3 cut(s) 3479, 3603, 3605
NaeI GCCGGC 1 cut(s) 2174
NciI CCSGG 1 cut(s) 3170
NcoI CCATGG 1 cut(s) 4334
NdeI CATATG 5 cut(s) 1087, 2001, 3409, 3687, 3834
NgoMIV GCCGGC 1 cut(s) 2172
NheI GCTAGC 1 cut(s) 5719
NlaIV GGNNCC 9 cut(s) 378, 759, 1816, 2165, 2322, 3754, 4144, 4273, 5301
NmeAIII GCCGAG 1 cut(s) 5737
NmuCI GTSAC 4 cut(s) 943, 1443, 3005, 3933
NruI TCGCGA 1 cut(s) 3479
NsiI ATGCAT 3 cut(s) 1057, 3343, 5472
NspI RCATGY 5 cut(s) 2756, 3552, 3737, 4442, 5423
NspV TTCGAA 5 cut(s) 2053, 2075, 3762, 4002, 5458
OliI CACNNNNGTG 1 cut(s) 312
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 124
PauI GCGCGC 1 cut(s) 3603
PceI AGGCCT 2 cut(s) 1246, 1303
PciI ACATGT 3 cut(s) 3733, 4438, 5419
PctI GAATGC 5 cut(s) 1422, 3847, 4627, 4666, 5729
PdiI GCCGGC 1 cut(s) 2174
PdmI GAANNNNTTC 3 cut(s) 1015, 1848, 4008
PflMI CCANNNNNTGG 4 cut(s) 194, 521, 4617, 5309
PfoI TCCNGGA 1 cut(s) 4012
PinAI ACCGGT 1 cut(s) 73
PleI GAGTC 4 cut(s) 1954, 2111, 2395, 2858
PpsI GAGTC 4 cut(s) 1954, 2111, 2395, 2858
PpuMI RGGWCCY 2 cut(s) 376, 1759
PscI ACATGT 3 cut(s) 3733, 4438, 5419
PshBI ATTAAT 1 cut(s) 1374
PsiI TTATAA 1 cut(s) 3588
Psp124BI GAGCTC 2 cut(s) 957, 2688
Psp1406I AACGTT 1 cut(s) 1109
Psp5II RGGWCCY 2 cut(s) 376, 1759
Psp6I CCWGG 9 cut(s) 244, 520, 1246, 2187, 2395, 3392, 3577, 3722, 4012
PspFI CCCAGC 5 cut(s) 4026, 4374, 4479, 4664, 5309
PspGI CCWGG 9 cut(s) 244, 520, 1246, 2187, 2395, 3392, 3577, 3722, 4012
PspN4I GGNNCC 9 cut(s) 378, 759, 1816, 2165, 2322, 3754, 4144, 4273, 5301
PspPI GGNCC 8 cut(s) 376, 1391, 1759, 2373, 4143, 4272, 4637, 5300
PspPPI RGGWCCY 2 cut(s) 376, 1759
PstI CTGCAG 4 cut(s) 1666, 2281, 2747, 3874
PsuI RGATCY 7 cut(s) 757, 1282, 2320, 2516, 2638, 2934, 3083
PteI GCGCGC 1 cut(s) 3603
PvuII CAGCTG 3 cut(s) 1495, 3485, 3674
RruI TCGCGA 1 cut(s) 3479
RseI CAYNNNNRTG 8 cut(s) 312, 2892, 4135, 4174, 4219, 4264, 4303, 4333
SacI GAGCTC 2 cut(s) 957, 2688
Sau96I GGNCC 8 cut(s) 376, 1391, 1759, 2373, 4143, 4272, 4637, 5300
ScaI AGTACT 2 cut(s) 4387, 5744
SchI GAGTC 4 cut(s) 1955, 2111, 2395, 2858
SduI GDGCHC 8 cut(s) 957, 1233, 1727, 2688, 4313, 4901, 4982, 5099
SfcI CTRYAG 7 cut(s) 465, 1183, 1662, 2277, 2743, 3870, 5674
Sfr274I CTCGAG 1 cut(s) 206
SfuI TTCGAA 5 cut(s) 2053, 2075, 3762, 4002, 5458
SinI GGWCC 6 cut(s) 376, 1391, 1759, 2373, 4272, 5300
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 8 cut(s) 312, 2892, 4135, 4174, 4219, 4264, 4303, 4333
SmlI CTYRAG 5 cut(s) 206, 666, 2377, 5052, 5130
SmoI CTYRAG 5 cut(s) 206, 666, 2377, 5052, 5130
SpeI ACTAGT 3 cut(s) 809, 4280, 4514
SseBI AGGCCT 2 cut(s) 1246, 1303
SspI AATATT 2 cut(s) 1138, 1577
SstI GAGCTC 2 cut(s) 957, 2688
StuI AGGCCT 2 cut(s) 1246, 1303
StyI CCWWGG 5 cut(s) 948, 2167, 4146, 4334, 4452
TaiI ACGT 4 cut(s) 312, 1112, 1350, 5278
TaqII GACCGA 2 cut(s) 2361, 5765
TatI WGTACW 7 cut(s) 134, 1129, 3950, 4283, 4385, 4517, 5742
TauI GCSGC 2 cut(s) 1454, 2759
TseFI GTSAC 4 cut(s) 943, 1443, 3005, 3933
Tsp45I GTSAC 4 cut(s) 943, 1443, 3005, 3933
TspGWI ACGGA 1 cut(s) 750
Van91I CCANNNNNTGG 4 cut(s) 194, 521, 4617, 5309
VpaK11BI GGWCC 6 cut(s) 376, 1391, 1759, 2373, 4272, 5300
VspI ATTAAT 1 cut(s) 1374
XceI RCATGY 5 cut(s) 2756, 3552, 3737, 4442, 5423
XcmI CCANNNNNNNNNTGG 3 cut(s) 640, 1528, 4503
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 3 cut(s) 1015, 1848, 4008
ZrmI AGTACT 2 cut(s) 4387, 5744
Zsp2I ATGCAT 3 cut(s) 1057, 3343, 5472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.