MD02G1287600.v1.1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006658
Physical Location & Seq
Reverse (-)
34424784 .. 34440963
16180 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1287600.v1.1.491

Sequence Viewer

Length: 5847 bp
ATGTTTCCCTGTCTCTTCCATGGCAGTTTCTTAAAGGAAATGGAGGAGGTCTCGACCTCGAATATTTTGGATGGGGAGATCGTTGGAATCAAATTTGGTTTAGCAACTCATCAAGAAATTTGTACAGCATCTATTAGCGATTGCTCTATCAGCCATGCAAGCCAGCTTTCCAACCCATTCCTTGGCCTGCCCCTTGAGTTTGGAAAGTGTGAATCTTGTGGCACTTCCGAACCTGGGAAATGTGAAGGTCATTTTGGGTATATTGAGTTACCAGTACCAATATTTCATCCCAATCATGTTAGCGAATTGAAGCGGATGCTGAGCTTATTGTGCTTGAAATGCTTGAAAATGAAAAAGAACAAGTTTCCAACCAAGAATGCTGGCTTAGCAGAAAGAATGTTAGCTTCATGTTGTGAGGATGCTGCACAAGTGTGTATTGGAGAGACTAAACCAACAGATAGCTCTTGTTCCTTGCAATTGAAGCGACCTTCTAAATCAAGAACACCACCTGGTTTTTGGAATTTCCTCGAACGATATGGTTTTCGTTATGGCGATGATCATATACGTACTTTGCTTCCTTGTGAGGTGATGGAGATGCTAAAGCGTATCCCTCAAGACACCAGGAAGAAACTTAATGCAAGAGGGTATTTTCCTCAAGATGGATACATCTTGCGTCATATACCTGTCCCTCCAAACTGTTTATCTGTTCCTGAAATTTCGGATGGTGTTAGTGTTATGTCTGCAGATCCTTCAATTTCAATGCTCAGAAAAGTTCTAAAGCAAATTGAAGTCATAAGAAGTTCAAGGTCAGGCATCCCAAACTTTGAATCACAAATTGTTGAAGCTAATGAACTTCAAGCCATAGTGGATCAGTATCTTCAAGTTAGGGGTACTGGGAAGGCATCACGGGATATAGATGCAAGGTTTGGGGTAAACAAGGAGCTGAATGCCTCTTCCACTAAAGCATGGCTTGAAAAAATGAGAACACTGTTCATTAGAAAGGGTTCAGGATTCTCTTCTCGCTCAGTAATCACTGGGGATGCCTTTAGAAGGGTAAATGAAGTTGGCATCCCCTATGAAATTGCTCAAAGAATCACCTTCGAGGAGAGGGTTAATGATCATAACATTAGGTACTTACAACAGTTGGTAGATAGCAAGTTGTGCCTAACCTATAGGGATGGATCATCAACTTACTCACTTAGGGAAGGTTCAAAGGGGCACACTTTTTTGAGGCCTGGTCAAGTGGTGCATCGGAGGATTATGGATGGGGACCTTGTATTTGTAAACAGGCCCCCAACTACCCATAAACATTCCCTACAAGCACTACAAGTGTATGTGCATGATGACCATGTGGTGAAAATAAATCCTTTGATATGTGGGCCACTAAGTGCTGACTTTGATGGTGATTGCATCCATTTGTTCTATCCGCAATCACTTGCTGCAAAAGCAGAAGTTTTGGAGCTTTTTTCAGTGGAGAAGCAGCTGCTTAGCTCTCATAGTGGAAAACCAAATCTGCAAATGGCCACTGATTCACTATTGTCATTGAGGATGATGTTTAAGAACTTTTTTTTGGATAAATCAGCAGCTCAGCAACTGGCCATGTTTGCTTCATCTTTGTTGCCACGACCTGCATTATTGAAAGCAAATGCTGCTTGCTCATATTGGACTGCTTTGCAGATATTACAGACTGCATTGCCTCTTGACTTTGACTACTGTGGGGAAAAGTACTTAGTCAATAAAAGTGAGATACTGAAAATTGAGTTCAGTACAAGTGCAGTTTCAGCGGTGATGAATGACATTGCTACTTCAGTTTTTTTTGAGAAAGGGGGCGAGGATGTCCTTAAATTCTTTGATTCTCTACAGCCCTTGCTGATGGAGAATATATTTTCAGAAGGGTTCAGTGTTGGCTTGGAGGATTTTTACATGTCAAAGACATCCATACAAGATATTAAGGAGAATATTCAGGGTACTTCAGATTTGTTGTATCATCTGAGGTCAACCTACAATGAATTTGTAGAGTTTCAGCTAGAGAGTCGAATCAAAAGCTTGAAAGTACCAGTTTCAAATTTTATCTTGGAGTCATCTGCATTCGGTGATTTAATCGACACCAAGAGTGATTCGGCAATCAACAAAGTTGTGCAACAAATTGGGTTCCTAGGCCTACAACTCTCTGATAAGGGAAGATTCTACTCAAAGACATTGGTTGAGGATGTGGCCTCTCTCTGTCACAACAAATATTCTTTTAACTCGGAGTATCCCTCTGCAGAATATGGCCTAGTTCAAAGTTGTTTCTTCCATGGGTTAGATCCATACGAGGAAATAGTTCACTCCATTGCTACACGAGAGGTGATTGTTCGGTCCTCAAGAGGATTGACTGAACCTGGGACCTTATTTAAAAATTTAATGGCCATTCTTCGTGATGTGGTTATTTGCTATGATGGCACAGTCAGAAATGTTTGTAGCAATTCAGTTATCCAATTTGAGTATGGAGTCAATGCTGGATCTAGGCCCCCGAATCTTTTCCCTGCTGGTGAACCTGTTGGCGTGTTAGCTGCCACTGCAATGTCAAATCCTGCGTACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCTTCATGGGAGCTGATGAAGGAAATTCTACTTTGCAAAGTAAATTTTAAGAATGAGCTCATTGATCGCCGTGTAATTTTGTACTTGAATAACTGTGGTTGTGGAAGAAAGTACTGCCGAGAACGTGCAGCATGTTTGGTTAAGAATCAGTTGAAGAAAGTCAGTCTTAAAGATACTGCAGTCGAATTCATGATTGAATATAGCAATCAGCTTTCTAGTTTGGGAAGTATGGTAAATGATGCAGGCCTAGTTGGCCATATTCATCTCAATGAGGAGCTGTTACGGGAGTTAAATGTTGGTGTAGACGAGATTCAACAAAAATGCCAAGAAACTGTTAATTCATTTCGGAGGAAGAGAGTTGGGAAGAAGAAGTTCAACATTGGCTATCTTTTCAAAAATACAGTTTTGTTTGCCAGTGAGCATTGCTCTTTTCATCATTCCTGTGCAGACAAAGGATCTGACTCCCCGTGCTTGATGTTCTTCCTTCAAGCTACTGAAGAGTTGGAAACCACTTCGCAATACTATGCTGATTTGATTTGCCCTGTGCTTTTAGAAACAATAATAAAAGGTGACCCACGGATTACCTCAGCAAATATAATTTGGATTGATCCCGATACAACAACTTGGATTAGAAGTCCAAATAATTCTCAAAAGGGTGAATGGGCCTTGGATGTTGTTCTGGAGAGATCTGTTGTCAAGCAAAGCGGTGATGCATGGAGAATCGTTCTCGATTCTTGCCTCCCTGTGCTTCACCTAATTGACACCAGGCGTTCCATCCCGTATGCTATCAAACAGATTCAAGAACTATTGGGAGTGTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCGACAGCTGTTACAATGGTGGCAAAAGGTGTCCTGAAAGAGCATCTTATCCTCCTGGCGAATAGCATGACATGTGCTGGAAATTTTGTTGGCTTCAATTCTAGTGGATATAAAGCACTATCTCGAGCATTGAATATTCAAGTACCATTTACAGAAGCAACACTTTTTACACCAAGAAGGTGCTTTGAGAGAGCAGCTGAAAAGTGCCACATGGATTCTTTGTCAAGCATAGTTGCATCATGTTCATGGGGTAAACATGTTGCTGTTGGTACTGGCTCCAGGTTTGATATCCTCTGGGACACAAGAGAGGGAGGATTGAATCAAGAGGGTGGACTAGATGTTTTCAACTTTCTGCATATGGTGAGCACAGCAAATGGAGAAGAAGCAACTACAGGAGCACTGGGGGCAGAAGTTGATGATCTTATGGTGGTAGATGAATTAGCAGATTCCTGCTTGTCGCCAGAACTTGACTCTGGTTTAGATAAGCCCGTCTTTGAAGATATTATAGAATTTGAGGAATCAGAAACTCTTCCTGGAAAGTCAAGTTGGGAAAAAGATTCCTCTGTATGGGGGACAAATCGTACAAGAGAAGATGCCACATCATCGTGGGATAAAAATGATACATCATCGTGGGATAAAACTCCAGCCAAAGAACCTGCCACATCTACATGGGGGATAGGCAAGGCAGCAGAAGTTACTACTCCTGCATGGAGGGCAAATACTGCTGGAGAAGCTTCCACATCTGCCTGGGGGGAAACTGGAGAGCCTACCACTTCTGCCTGGGGGGCAAGTAAGGGAGGTGAAAATGATTGGAGTGGTAGAGTGGCAGAGAAAGTTGCACCAATAGACCTCCAGCCGACAATAAAAGCCGTAGATGAAAAGGACAATGGATGGGGTGCTGTTTCTCCTAGGGAAACAGAAACAGGGAATGACAATGCTTGGTCTAGTGGGCAACAGAAAAAGTCTTCTGAAATTTCGACCAGTTCTTGGGGGCAGAAAAATCCTCCTGAGACAGAAACTCAACCTTGGGGTGCTGTTTCTTCTGGGGAAACAGAAACAGGGAATGACAATGCTTGGTCCAGTGGGCAACAGAAAAAATCTTCTGAAATTTCTACCAGTTCTTGGGGGCAGAAAAATGCTTGGTCTAGTGGGCAACAGAAAAAGTCTTCTGAAATTTCGACCAGTTCTTGGGGGCAGAAAAATCCTCCTGAGACAGAAACTCAACCTTGGGGTGCTGTTTCTTCTGGGGAAACAGAAACAGGGAATGCCAATGCTTGGTCTAGTGGGCAGCAGAAAAAGTCTTCTGAAATTTCGACCAGTTCTTGGGGGCAGAAAAATCCTCCTGAGACAGAAACTCAACCTTGGGGTGCTGTTTCTTATGGGGAAACAGAAACAGGGAATGACAAGGCTTGGTCTAGTGGGCAACAGAAAAAGTCTTCTGAAATTTCGACCAGTTCTTGGGGGCAGAAAAATCCTCCTGTGATAGAAACTCAACCTTGGGGTGCTGTTTCATCTGGGGAAACAGAATCAGGGAATGACAATGCTTGGTCTAGTGGGCAACAGAAAAAATCTTCCGAAATTTCTACCAGTTCTTGGGGGCAGAAAAATCCTCCTGAGACAGAAACTCAACCTGCGAGCTTGTCTGGGTGGGATTCACCTAGTGGAGATGGTAATACTGGTGAAAGACATCACCAATGGGGGCAGAACAAGAAAAGTCGTTTTGAAGGTTCAAAGAGTTGGGTTTCTAGTCCCGGGGAGTGGAAGAATAAGAACCGTCCTGCCAAACCACCTGGTATGGTGAATGATAACTCTAGTGTGGTTGGACTCTATACTGCAACAAGACAAAGATTGGATATGTTCCCTAGCGAGGAGCAAGACGTTCTTTCTAAAATTGAACCTGTTATGCGCTCTATCCGAAGAATAATGCATCAATCTGGTTTGTACAATGATGGGGATCCACTGTCAGCTGACGACCAGTCATTTATACTTGATAATGTTCTCAATTACCACCCGGATAAAGCTGTTAAAATGGGTTCTGGAATTGGTCATCTCACAGTTGATAGGCACGGCAGTTTCCAGGACAGCAGATGCTTTTTTGTTGTGACAACAGATGGTCACAAGGAAGATTTTTCATATCGAAAATGCCTGGAGAATTACATTAAGGAAAAGTATGCTGATGTTGCGGAAACATTCATAGGCAAGTATTTTACTAGGAGAGGAGGGAATCGGGAGCGAAACCCCACTCCAAGTCAATATCCCATTCCAGAACAGACTCCATGTCAAACTCCCATTGCAGAGCAAACTATAAGTCAAACTCCCATTGCAGAGCAAACTCTAAGTCAAACCCCCATTTCAGAGCAAACTGGGAACGAGGAGACACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1949

Amino Acids

215.6

Weight (kDa)

5.64

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 59 - 294 1.1e-11 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 337 - 491 6.9e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 494 - 639 1.1e-13 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 767 - 1194 2.4e-09 RNA polymerase Rpb1, domain 5
DUF3223 PF11523 1794 - 1870 3.3e-27 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1636, 4126, 5101
AccB7I CCANNNNNTGG 8 cut(s) 182, 4449, 4584, 4650, 4737, 4785, 4920, 5055
AccI GTMKAC 1 cut(s) 2916
AccII CGCG 1 cut(s) 3464
AciI CCGC 5 cut(s) 313, 1427, 1784, 3318, 5645
AclWI GGATC 9 cut(s) 740, 876, 1189, 2300, 2509, 3076, 3215, 5411, 5424
AcoI YGGCCR 4 cut(s) 1521, 1596, 2406, 2866
AcuI CTGAAG 3 cut(s) 1791, 1956, 3129
AdeI CACNNNGTG 2 cut(s) 1388, 5123
AflIII ACRYGT 3 cut(s) 1923, 3533, 3718
AhdI GACNNNNNGTC 2 cut(s) 5437, 5739
AjuI GAANNNNNNNTTGG 6 cut(s) 237, 269, 1553, 1585, 3991, 4023
AleI CACNNNNGTG 1 cut(s) 430
Alw21I GWGCWC 3 cut(s) 2673, 3830, 3862
Alw26I GTCTC 8 cut(s) 17, 55, 437, 4466, 4667, 4802, 5072, 5831
AlwI GGATC 9 cut(s) 740, 876, 1189, 2300, 2509, 3076, 3215, 5411, 5424
AlwNI CAGNNNCTG 1 cut(s) 1594
Ama87I CYCGRG 2 cut(s) 3585, 5213
ArsI GACNNNNNNTTYG 6 cut(s) 1262, 1294, 5755, 5785, 5787, 5817
Asp700I GAANNNNTTC 5 cut(s) 1003, 2322, 2519, 2984, 3990
AspA2I CCTAGG 2 cut(s) 2155, 4370
AspLEI GCGC 2 cut(s) 3459, 5370
AspS9I GGNCC 8 cut(s) 1270, 1290, 1379, 2358, 2385, 2508, 3276, 4539
AsuC2I CCSGG 3 cut(s) 5214, 5215, 5474
AvaI CYCGRG 2 cut(s) 3585, 5213
AvaII GGWCC 4 cut(s) 1270, 2358, 2385, 4539
AvrII CCTAGG 2 cut(s) 2155, 4370
BaeGI GKGCMC 1 cut(s) 1221
BaeI ACNNNNGTAYC 2 cut(s) 2778, 2811
BalI TGGCCA 4 cut(s) 1523, 1598, 2408, 2868
BamHI GGATCC 1 cut(s) 5416
BanII GRGCYC 1 cut(s) 2673
BauI CACGAG 1 cut(s) 2340
BbsI GAAGAC 4 cut(s) 4419, 4620, 4755, 4890
Bbv12I GWGCWC 3 cut(s) 2673, 3830, 3862
BbvCI CCTCAGC 1 cut(s) 3199
BceAI ACGGC 3 cut(s) 2667, 4316, 5545
BciVI GTATCC 3 cut(s) 617, 656, 2265
BclI TGATCA 3 cut(s) 556, 1117, 3442
BcnI CCSGG 3 cut(s) 5214, 5215, 5474
BcoDI GTCTC 8 cut(s) 17, 55, 437, 4466, 4667, 4802, 5072, 5831
BfmI CTRYAG 6 cut(s) 741, 1171, 1859, 2262, 2790, 3852
BfoI RGCGCY 1 cut(s) 3460
BfuAI ACCTGC 3 cut(s) 1636, 4126, 5101
BfuI GTATCC 3 cut(s) 617, 656, 2265
BglI GCCNNNNNGGC 2 cut(s) 2865, 4247
BglII AGATCT 1 cut(s) 3299
BlnI CCTAGG 2 cut(s) 2155, 4370
BlpI GCTNAGC 4 cut(s) 320, 385, 1487, 1587
BmcAI AGTACT 2 cut(s) 1727, 2726
Bme18I GGWCC 4 cut(s) 1270, 2358, 2385, 4539
BmeRI GACNNNNNGTC 2 cut(s) 5437, 5739
BmeT110I CYCGRG 2 cut(s) 3585, 5213
BmgT120I GGNCC 8 cut(s) 1270, 1290, 1379, 2358, 2385, 2508, 3276, 4539
BmiI GGNNCC 7 cut(s) 1271, 1292, 2153, 2386, 2510, 3739, 5418
BmrI ACTGGG 4 cut(s) 903, 1044, 3872, 5835
BmuI ACTGGG 4 cut(s) 903, 1044, 3872, 5835
BpiI GAAGAC 4 cut(s) 4419, 4620, 4755, 4890
BplI GAGNNNNNCTC 6 cut(s) 35, 67, 2243, 2275, 3023, 3055
BpmI CTGGAG 7 cut(s) 3314, 3724, 4089, 4209, 4242, 4298, 5630
Bpu10I CCTNAGC 1 cut(s) 3199
Bpu1102I GCTNAGC 4 cut(s) 320, 385, 1487, 1587
BpuEI CTTGAG 4 cut(s) 215, 597, 639, 2347
BpuMI CCSGG 3 cut(s) 5214, 5215, 5474
BsaAI YACGTR 1 cut(s) 566
BsaBI GATNNNNATC 2 cut(s) 873, 5415
BsaI GGTCTC 1 cut(s) 55
Bse3DI GCAATG 7 cut(s) 1691, 1797, 2331, 2568, 3034, 5751, 5781
Bse8I GATNNNNATC 2 cut(s) 873, 5415
BseJI GATNNNNATC 2 cut(s) 873, 5415
BseMI GCAATG 7 cut(s) 1691, 1797, 2331, 2568, 3034, 5751, 5781
BseRI GAGGAG 5 cut(s) 59, 1118, 2900, 5345, 5694
BseSI GKGCMC 1 cut(s) 1221
BsgI GTGCAG 4 cut(s) 408, 1794, 2760, 3078
Bsh1236I CGCG 1 cut(s) 3464
BsiHKAI GWGCWC 3 cut(s) 2673, 3830, 3862
BsiHKCI CYCGRG 2 cut(s) 3585, 5213
BsiSI CCGG 2 cut(s) 5214, 5474
BslFI GGGAC 6 cut(s) 671, 1283, 2398, 3773, 4048, 5196
BsmAI GTCTC 8 cut(s) 17, 55, 437, 4466, 4667, 4802, 5072, 5831
BsmFI GGGAC 6 cut(s) 671, 1283, 2398, 3773, 4048, 5196
BsmI GAATGC 4 cut(s) 382, 952, 2087, 4732
Bso31I GGTCTC 1 cut(s) 55
BsoBI CYCGRG 2 cut(s) 3585, 5213
Bsp1286I GDGCHC 4 cut(s) 1221, 2673, 3830, 3862
Bsp1407I TGTACA 2 cut(s) 122, 5403
Bsp1720I GCTNAGC 4 cut(s) 320, 385, 1487, 1587
Bsp19I CCATGG 2 cut(s) 19, 2296
Bsp68I TCGCGA 1 cut(s) 3464
BspACI CCGC 5 cut(s) 313, 1427, 1784, 3318, 5645
BspFNI CGCG 1 cut(s) 3464
BspHI TCATGA 1 cut(s) 2802
BspLI GGNNCC 7 cut(s) 1271, 1292, 2153, 2386, 2510, 3739, 5418
BspMAI CTGCAG 3 cut(s) 745, 2266, 2794
BspMI ACCTGC 3 cut(s) 1636, 4126, 5101
BspPI GGATC 9 cut(s) 740, 876, 1189, 2300, 2509, 3076, 3215, 5411, 5424
BspTNI GGTCTC 1 cut(s) 55
BsrDI GCAATG 7 cut(s) 1691, 1797, 2331, 2568, 3034, 5751, 5781
BsrGI TGTACA 2 cut(s) 122, 5403
BssSI CACGAG 1 cut(s) 2340
Bst2BI CACGAG 1 cut(s) 2340
Bst6I CTCTTC 7 cut(s) 20, 958, 1021, 2620, 2960, 3105, 3996
BstAPI GCANNNNNTGC 3 cut(s) 1161, 1649, 4184
BstAUI TGTACA 2 cut(s) 122, 5403
BstBAI YACGTR 1 cut(s) 566
BstC8I GCNNGC 7 cut(s) 160, 164, 188, 382, 1654, 2857, 5098
BstDSI CCRYGG 3 cut(s) 19, 2296, 3188
BstEII GGTNACC 1 cut(s) 3182
BstENI CCTNNNNNAGG 1 cut(s) 1048
BstFNI CGCG 1 cut(s) 3464
BstH2I RGCGCY 1 cut(s) 3460
BstHHI GCGC 2 cut(s) 3459, 5370
BstMAI GTCTC 8 cut(s) 17, 55, 437, 4466, 4667, 4802, 5072, 5831
BstNSI RCATGY 4 cut(s) 1927, 2748, 3537, 3722
BstPI GGTNACC 1 cut(s) 3182
BstSFI CTRYAG 6 cut(s) 741, 1171, 1859, 2262, 2790, 3852
BstSLI GKGCMC 1 cut(s) 1221
BstSNI TACGTA 1 cut(s) 566
BstUI CGCG 1 cut(s) 3464
BstV2I GAAGAC 4 cut(s) 4419, 4620, 4755, 4890
BstX2I RGATCY 6 cut(s) 745, 2305, 2501, 3068, 3299, 5416
BstYI RGATCY 6 cut(s) 745, 2305, 2501, 3068, 3299, 5416
BsuI GTATCC 3 cut(s) 617, 656, 2265
BtgI CCRYGG 3 cut(s) 19, 2296, 3188
BtgZI GCGATG 1 cut(s) 567
BtsI GCAGTG 1 cut(s) 2556
BtuMI TCGCGA 1 cut(s) 3464
BveI ACCTGC 3 cut(s) 1636, 4126, 5101
Cac8I GCNNGC 7 cut(s) 160, 164, 188, 382, 1654, 2857, 5098
CaiI CAGNNNCTG 1 cut(s) 1594
CciI TCATGA 1 cut(s) 2802
CfoI GCGC 2 cut(s) 3459, 5370
Cfr13I GGNCC 8 cut(s) 1270, 1290, 1379, 2358, 2385, 2508, 3276, 4539
Cfr9I CCCGGG 1 cut(s) 5213
CseI GACGC 1 cut(s) 662
CsiI ACCWGGT 2 cut(s) 508, 5251
CspCI CAANNNNNGTGG 2 cut(s) 3547, 3582
DraI TTTAAA 1 cut(s) 2395
DraIII CACNNNGTG 2 cut(s) 1388, 5123
DriI GACNNNNNGTC 2 cut(s) 5437, 5739
EaeI YGGCCR 4 cut(s) 1521, 1596, 2406, 2866
Eam1104I CTCTTC 7 cut(s) 20, 958, 1021, 2620, 2960, 3105, 3996
Eam1105I GACNNNNNGTC 2 cut(s) 5437, 5739
EarI CTCTTC 7 cut(s) 20, 958, 1021, 2620, 2960, 3105, 3996
Ecl136II GAGCTC 1 cut(s) 2671
Eco105I TACGTA 1 cut(s) 566
Eco147I AGGCCT 3 cut(s) 1234, 2160, 2859
Eco24I GRGCYC 1 cut(s) 2673
Eco31I GGTCTC 1 cut(s) 55
Eco32I GATATC 1 cut(s) 3751
Eco47I GGWCC 4 cut(s) 1270, 2358, 2385, 4539
Eco53kI GAGCTC 1 cut(s) 2671
Eco57I CTGAAG 3 cut(s) 1791, 1956, 3129
Eco88I CYCGRG 2 cut(s) 3585, 5213
Eco91I GGTNACC 1 cut(s) 3182
EcoICRI GAGCTC 1 cut(s) 2671
EcoNI CCTNNNNNAGG 1 cut(s) 1048
EcoO109I RGGNCCY 4 cut(s) 1270, 1290, 2385, 2508
EcoO65I GGTNACC 1 cut(s) 3182
EcoRI GAATTC 1 cut(s) 2798
EcoRV GATATC 1 cut(s) 3751
EcoT22I ATGCAT 2 cut(s) 3328, 5391
EcoT38I GRGCYC 1 cut(s) 2673
FaqI GGGAC 6 cut(s) 671, 1283, 2398, 3773, 4048, 5196
FauNDI CATATG 1 cut(s) 3819
FbaI TGATCA 3 cut(s) 556, 1117, 3442
FblI GTMKAC 1 cut(s) 2916
FriOI GRGCYC 1 cut(s) 2673
GlaI GCGC 2 cut(s) 3458, 5369
GsuI CTGGAG 7 cut(s) 3314, 3724, 4089, 4209, 4242, 4298, 5630
HaeII RGCGCY 1 cut(s) 3460
HapII CCGG 2 cut(s) 5214, 5474
HgaI GACGC 1 cut(s) 662
HhaI GCGC 2 cut(s) 3459, 5370
Hin6I GCGC 2 cut(s) 3457, 5368
HinP1I GCGC 2 cut(s) 3457, 5368
HincII GTYRAC 1 cut(s) 1998
HindII GTYRAC 1 cut(s) 1998
HindIII AAGCTT 2 cut(s) 2044, 4194
HpaII CCGG 2 cut(s) 5214, 5474
Hpy166II GTNNAC 8 cut(s) 934, 1285, 1998, 2326, 2534, 2917, 3716, 3794
Hpy8I GTNNAC 8 cut(s) 934, 1285, 1998, 2326, 2534, 2917, 3716, 3794
HpyCH4IV ACGT 3 cut(s) 565, 2737, 5340
HpySE526I ACGT 3 cut(s) 565, 2737, 5340
HspAI GCGC 2 cut(s) 3457, 5368
Ksp22I TGATCA 3 cut(s) 556, 1117, 3442
LmnI GCTCC 8 cut(s) 940, 1459, 2623, 2887, 3743, 3857, 5332, 5692
MabI ACCWGGT 2 cut(s) 508, 5251
MaeII ACGT 3 cut(s) 565, 2737, 5340
MaeIII GTNAC 8 cut(s) 267, 2225, 2892, 3182, 3472, 4156, 5563, 5576
MfeI CAATTG 1 cut(s) 476
MflI RGATCY 6 cut(s) 745, 2305, 2501, 3068, 3299, 5416
MhlI GDGCHC 4 cut(s) 1221, 2673, 3830, 3862
MlsI TGGCCA 4 cut(s) 1523, 1598, 2408, 2868
MluNI TGGCCA 4 cut(s) 1523, 1598, 2408, 2868
MlyI GAGTC 7 cut(s) 2041, 2087, 2499, 3068, 3926, 5280, 5728
MmeI TCCRAC 5 cut(s) 64, 195, 392, 3096, 5263
Mox20I TGGCCA 4 cut(s) 1523, 1598, 2408, 2868
Mph1103I ATGCAT 2 cut(s) 3328, 5391
MroXI GAANNNNTTC 5 cut(s) 1003, 2322, 2519, 2984, 3990
MscI TGGCCA 4 cut(s) 1523, 1598, 2408, 2868
MslI CAYNNNNRTG 8 cut(s) 430, 2561, 2880, 3054, 3706, 4066, 4090, 4129
Msp20I TGGCCA 4 cut(s) 1523, 1598, 2408, 2868
MspA1I CMGCKG 5 cut(s) 1483, 1784, 3470, 3659, 5429
MspI CCGG 2 cut(s) 5214, 5474
MunI CAATTG 1 cut(s) 476
Mva1269I GAATGC 4 cut(s) 382, 952, 2087, 4732
MvnI CGCG 1 cut(s) 3464
NciI CCSGG 3 cut(s) 5214, 5215, 5474
NcoI CCATGG 2 cut(s) 19, 2296
NdeI CATATG 1 cut(s) 3819
NlaIV GGNNCC 7 cut(s) 1271, 1292, 2153, 2386, 2510, 3739, 5418
NmeAIII GCCGAG 1 cut(s) 2756
NmuCI GTSAC 4 cut(s) 2225, 3182, 5563, 5576
NruI TCGCGA 1 cut(s) 3464
NsiI ATGCAT 2 cut(s) 3328, 5391
NspI RCATGY 4 cut(s) 1927, 2748, 3537, 3722
OliI CACNNNNGTG 1 cut(s) 430
PaeR7I CTCGAG 1 cut(s) 3585
PagI TCATGA 1 cut(s) 2802
PceI AGGCCT 3 cut(s) 1234, 2160, 2859
PciI ACATGT 3 cut(s) 1923, 3533, 3718
PctI GAATGC 4 cut(s) 382, 952, 2087, 4732
PdmI GAANNNNTTC 5 cut(s) 1003, 2322, 2519, 2984, 3990
PflMI CCANNNNNTGG 8 cut(s) 182, 4449, 4584, 4650, 4737, 4785, 4920, 5055
PfoI TCCNGGA 2 cut(s) 3994, 5538
PleI GAGTC 7 cut(s) 2040, 2086, 2498, 3068, 3926, 5280, 5728
PpsI GAGTC 7 cut(s) 2040, 2086, 2498, 3068, 3926, 5280, 5728
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 2 cut(s) 1270, 2385
PscI ACATGT 3 cut(s) 1923, 3533, 3718
Psp124BI GAGCTC 1 cut(s) 2673
Psp5II RGGWCCY 2 cut(s) 1270, 2385
PspEI GGTNACC 1 cut(s) 3182
PspN4I GGNNCC 7 cut(s) 1271, 1292, 2153, 2386, 2510, 3739, 5418
PspPI GGNCC 8 cut(s) 1270, 1290, 1379, 2358, 2385, 2508, 3276, 4539
PspPPI RGGWCCY 2 cut(s) 1270, 2385
PstI CTGCAG 3 cut(s) 745, 2266, 2794
PstNI CAGNNNCTG 1 cut(s) 1594
PsuI RGATCY 6 cut(s) 745, 2305, 2501, 3068, 3299, 5416
PvuII CAGCTG 4 cut(s) 1483, 3470, 3659, 5429
RruI TCGCGA 1 cut(s) 3464
RseI CAYNNNNRTG 8 cut(s) 430, 2561, 2880, 3054, 3706, 4066, 4090, 4129
SacI GAGCTC 1 cut(s) 2673
Sau96I GGNCC 8 cut(s) 1270, 1290, 1379, 2358, 2385, 2508, 3276, 4539
ScaI AGTACT 2 cut(s) 1727, 2726
SchI GAGTC 7 cut(s) 2041, 2087, 2499, 3068, 3926, 5280, 5728
SduI GDGCHC 4 cut(s) 1221, 2673, 3830, 3862
SexAI ACCWGGT 2 cut(s) 508, 5251
SfcI CTRYAG 6 cut(s) 741, 1171, 1859, 2262, 2790, 3852
SfiI GGCCNNNNNGGCC 1 cut(s) 2865
Sfr274I CTCGAG 1 cut(s) 3585
SinI GGWCC 4 cut(s) 1270, 2358, 2385, 4539
SlaI CTCGAG 1 cut(s) 3585
SmaI CCCGGG 1 cut(s) 5215
SmiMI CAYNNNNRTG 8 cut(s) 430, 2561, 2880, 3054, 3706, 4066, 4090, 4129
SmlI CTYRAG 5 cut(s) 194, 612, 654, 2362, 3585
SmoI CTYRAG 5 cut(s) 194, 612, 654, 2362, 3585
SnaBI TACGTA 1 cut(s) 566
SseBI AGGCCT 3 cut(s) 1234, 2160, 2859
SsiI CCGC 5 cut(s) 313, 1427, 1784, 3318, 5645
SspI AATATT 5 cut(s) 64, 282, 1960, 2237, 3598
SstI GAGCTC 1 cut(s) 2673
StuI AGGCCT 3 cut(s) 1234, 2160, 2859
TaiI ACGT 3 cut(s) 568, 2740, 5343
TaqII GACCGA 1 cut(s) 2346
TatI WGTACW 6 cut(s) 122, 1725, 1766, 2694, 2724, 5403
TseFI GTSAC 4 cut(s) 2225, 3182, 5563, 5576
Tsp45I GTSAC 4 cut(s) 2225, 3182, 5563, 5576
TspGWI ACGGA 1 cut(s) 3205
TspMI CCCGGG 1 cut(s) 5213
Van91I CCANNNNNTGG 8 cut(s) 182, 4449, 4584, 4650, 4737, 4785, 4920, 5055
VpaK11BI GGWCC 4 cut(s) 1270, 2358, 2385, 4539
XagI CCTNNNNNAGG 1 cut(s) 1048
XceI RCATGY 4 cut(s) 1927, 2748, 3537, 3722
XcmI CCANNNNNNNNNTGG 2 cut(s) 513, 2483
XhoI CTCGAG 1 cut(s) 3585
XmaI CCCGGG 1 cut(s) 5213
XmaJI CCTAGG 2 cut(s) 2155, 4370
XmiI GTMKAC 1 cut(s) 2916
XmnI GAANNNNTTC 5 cut(s) 1003, 2322, 2519, 2984, 3990
ZrmI AGTACT 2 cut(s) 1727, 2726
Zsp2I ATGCAT 2 cut(s) 3328, 5391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.