Rroxscaffold_4G00325860

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006658
Physical Location & Seq
Reverse (-)
57793316 .. 57809063
15748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325860.1

Sequence Viewer

Length: 5157 bp
ATGGAGGAAGCGTCTTGTTCACCGGTTTTAGATGGGGAGTTAGTTGGAATTAAATTTGGTTTGGCAACTCATCATGAAATTTGTACAGCATCGATGATGGGATGCTCTATTAGCCATGCCAGTCAGCTTTCCAACCCATTTCTTGGCCTTCCTCTCGAGTTTGGGAAGTGTGAATCTTGTGGCACTTCCGAACCTGGAAAGTGCGAAGGGCATTTTGGATATATCGACTTACCATTACCGATATATCATCCTAACCACGTCAGTGAGCTGAAGCGCTTGCTGAGTTTTTTGTGCCTGAAGTGCTTAAAAATGAAAAAGAACAAGGTCCCAAGCAAGAGTGCTGGTTTGGCCGAAAGATTGTTATCTGTATGTTGTGAGGACGCTTCACAAGTTTCTATCGCAGAGATTAAACCTACAGATGGGGCTTGCTCCTTGCAATTGAAACTACCTTCTAATAAAAAACCACCACCTGGTTTTTGGAATTTCCTAGAAAGATATGGTTTTCGTTATGGTGATGGCGTGAAACGCACTTTGCTTCCTAGAGAGGTGATGCAGATTCTCAAAAGAATCCCTGAAGACACCAGAAGAAAACTGGCTGCTAAAGGCTATAATCCTCAAGACGGATACATCTTGAATCATATACCTGTCCCTCCTAATTGCTTGTCTGTGCCTGAAATATCTGATGGTGTTACTGTTATGTCTGCGGATCCTTCAATATCAATGCTCAAAAAAGTTCTAAGGCAAGTTGAAATGATCACTAGTTCAAGGTCTGGTACAGCAAATTTTGAATCTGAAATTGTTGAGGCGAATGAGTTACAAGCAATTGTTGATCAGTATCTTCAAGTTAGGGGTACTGGAAAGGCATCCCGAGATATTGATGCAAGATTTGGAGTGACCAAGGAGCTCAACAATTCTTCAACAAAAGCATGGCTTGAAAAGATGAGGACATTGTTCATCAGAAAGGGTTCAGGTTTCTCTTCCCGCACAGTAATTACTGGGGATGCATATAGAAGGGTGAATGAAGTTGGTATCCCATATGAAATTGCTCAAAGAATAACATTCGAGGAGAAGGTCAATGTACATAATATTAGGCGTCTGCAAGAGCTGGTAGATAGCAAGTTATGCCTAACCTACAGCGATGGTTCATCAACTTACTCTTTAAGGGAAGGTTCAAAGGGGCACACATTTCTGAGGCCTGGTCAAGTGGTGCATAGGAGGATAATGGATGGGGATCTTGTTTTCGTAAATAGGCCTCCAACTACCCATAAACATTCACTTCAAGCACTTCAAGTTTACGTCCATGATGACCATGTGGTCAAGATTAATCCTCTAATATGTGGACCACTAAGTGCTGACTTTGATGGAGACTGCATTCATTTGTTCTATCCCCAGTCACTTGCGGCAAAGGCAGAAGTTATGGAGCTTTTTTCAGTGGAGAAACAGCTGCTTAGTTCTCACAGTGGCAAGCCCAATTTGCAACTGGCCACCGATTCACTTTTGTCATTAAAAATGATGTTTAAGAAATATTTTTTCGACAAAGCAGCAGCGCAGCAATTGGCCATGTTTGTTTCATCTTCATTGCCACAACCAGCTTTATTGAAAGCTAATTCTGCAGTTCCATATTGGACTGCTTTGCAGATATTGCAGACTGCATTGCCTGCACATTTTGAGTGCTCTGGAGACAGGCACTTGGTCAGTGCTAGTGAGGTCCTCAAACTTGATTGTAATACAAGTCTCATACCAGCTCTTATCAATGACATTGGTACCTCTATTTTCTTCGAGAAAAGTGCTGAAGATGTTCTCAATTTCTTTAATTCTATGCAGCCATTGCTGATGGAGAATTTATTTTCAGAAGGGTTCAGTGTAGGTTTGGAGGATTTTGCCATGTCTAGGGAGTCCATTCAGGAGATTAGAAAGGGACTTAAAATCATTTCTCCTTTGCTGTATCATATGAGGGAAACATATAATGAGCTTGTTGAGTTGCAACTGGAGAATCACATTCGAACGGTGAAACAACCAGTTTCGAATTTTATATTAAATTCATCGGCATTGGGTGATTTAATTGACTCTAAGAGTGATTCAGCCATGAACAAAGTAACTCAACAAGTTGGGTTCCTTGGCCGGCAACTTTATGACCAGGGGAAACTCTACTCAAAAACATTGGTTGAGGATGTGAGCTCTCTGTATCAGAGCAAGTATCCATCTGACATCGTGGATTATCCCTCTGCAGAATTCGGATTAGTTCATAGTGGTTTTGTTCATGGGTTGGATCCTGTTGAGGGAATGATTCATTCCATTGCTACAAGGGAAGTAATTGTTCGGTCCTCAAGAGGGTTGTCTGAACCTGGGACTCTTTTCAAAAATCTAATGGCTACTCTCCGAGATGTTGTCATTTGCTATGATGGCACAGTGAGGAATGTGTGCAGTAATTCTATAATTCAGTTCGAGTATGGAGTAAAGAGCGGATCTAGTCCACAAAATTTGTTCCCTGCCGGTGAACCTGTGGGTGTGTTAGCTGCAACAGCAATGTCAAATCCAGCATACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCTTCATGGGATCTGATGAAGGAAATACTGCTCTGCAAAGTCAGTTTCAAGAACGAGCTCATTGATCGTCGTGTAATCTTGTATCTGAATGACTGTGGTTGCGGAAGAAACTACTGCAGAGAACATGCAGCATATTTGGTCAAGAATCAGTTGAAAAAAGTCAGCCTTAAAGATACTGCAGTAGAGTTTATGATTGAATATCAAAATCAGCGAGCTGCGGGAAGCATGGAAATTGACTCTGGCCTTGTTGGTCATGTTCATTTGAATGAGATGCTGTTACGGGAGTTAAATCTGGGAATGTATGAGATCCTTCAAAAATGCGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTTGGGAAGAAGATGAACATCGGTGACATTTTTAAAAGGACAGTTTTATCGTACAGTGAATGTTGTTATTTTCATCAATCCTCTGCTGACAATAGATCTGGATCTCCTTGCTTGATGTTCTTCTACCAAGATTTTAATAATTCAGAGTTGGAGGCCATTTCACAAATCTTGGCTGACTTTATTTGCCCAGTTCTTCTAAAAACAGTAATTAAAGGTGATCCTCGAATTTCGTCGGCGAACATAATTTGGATCAACTCAGAATCAACAACTTGGATAAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCATGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATGTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAGGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCGACAGCTGTTGCAATGGTGGCAAAAGGTGTTCTCAAAGAGCATCTTATTCTCTTGGCAAACAGTATGACATGCGCTGGAAATTTTGTTGGTTTCAATCCTGGTGGTTATAAAGCACTATCTCGCGCATTGAATATACAAGTACCCTTTACAGAAGCAACATTGTTCACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCTTTGTCAAGCATAGTTGCATCATGTTCCTGGGGTAAACATGTAGCTGTTGGTACTGGCTCCAGATTCGAAATCCTCTGGGACACAAGAGAGGGAGGTTTGAATGAAGAGGGTGGGATAGATGTTTTCAACTTTCTACATATGGTGAATGCTGCAAATGGAGAAGAATCAACTACTGCAGCTTTGGGAGCAGAAATTGACGATCTTGTGCCGGAATATGAAAATGGAGATGTGTCGATGTCACCAGAACATAACTGTACTTCTGATAAGCCAGTGTTTGAAGATATTGTTGAATTCCCGGATAGTTTCGAAAATGTTCCTGGAAAATCAAGCTGGGATTCATCTCTCAACACCGCTTCTTCTGGTGGGAAAGACTGGGGGGCAGTTAAAGTTGGAAGACAAGATAGCATTTCAGCAGAAACCCAAGTGGATAGCACATCTGCATGGGGGGCCAAGGCTATTACAGAAGATGCCCCATCTGTATGGGGAACAGCCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCATACAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGATGCCCCATCTGCATGGGGGACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACGGCCAAAACTGCTACATCTCCTTGGGAGACCAACACTGGTAGAGAAGATTCCACATCGGGCTGGGGGACAAGTACTGCCAGAGAAGATGCCACGACGGCATGGGGGAAAAGCAATGAAAAGAAACATGTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTTGCTGGGGGACAGAAAATCCACAATCTACATGGAGGACAGAAAGCGAGAATGGTTGGTCTGGTGGTGGGGTGAGTAAAGCTGAATCAACAGACGTCCAGTCCAAGAAAGCACAAGAAGATTCTACAGCCAATGGTTGGAATGCCTTTTCTGCTGGGCCAACAAAACCCCAAAAGGAGAATGCTGGGTCTGGATGGGGCATGAAAGGTTTTGATAAGAAAGATGGTAATGAGTTGGAACAAGATAAGTGTACTGCCCGTCATTCTTGGGGGCAGCAAAAGCCCGCCGGTACTTCTCAGGGCCCTTGGGCACAACAAAAGTCCCCTGAGACTTCTAAGGGTGCTTGGGGGCAGCAAAATGCCGCTACTTCAGAAGGTGCTTGGGGTCAGGAAAAGCCTGCCGATACTTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCTGAGATATCTGAAGGCGCTTGGGGGAGCAAAAGCGTGCTGATGCTAATTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCAGATATTTCTGAAGGCGCATGGGGGCAGCAAAAGCCTGATACAACTTCTCAAGGCTCTTGGGGACAGCAAAAGTCTCCTGAAATGTCTCAGGGCACTTGGGGAAGGCAAAAGTCTCCTGATATTTCTCAAGGCGCATGGGGGCAACAAAAGCCTTCTGATACTTCTCAACCTGCAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1718

Amino Acids

188.39

Weight (kDa)

6.35

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 48 - 281 1.6e-10 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 324 - 478 4.5e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 481 - 626 5.5e-10 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 755 - 1181 6.3e-10 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3537
AasI GACNNNNNNGTC 1 cut(s) 1313
AatII GACGTC 1 cut(s) 4554
Acc65I GGTACC 1 cut(s) 1763
AccB1I GGYRCC 1 cut(s) 1763
AccB7I CCANNNNNTGG 3 cut(s) 143, 470, 4593
AccBSI CCGCTC 1 cut(s) 2463
AccII CGCG 2 cut(s) 3428, 3552
AciI CCGC 9 cut(s) 704, 982, 1400, 2463, 2679, 2795, 3996, 4740, 4818
AcoI YGGCCR 5 cut(s) 348, 1482, 1557, 2119, 4296
AcsI RAATTY 3 cut(s) 127, 184, 288
AcuI CTGAAG 8 cut(s) 290, 317, 594, 1812, 3248, 4809, 4926, 5006
AcyI GRCGYC 2 cut(s) 1093, 4551
AdeI CACNNNGTG 1 cut(s) 1349
AfeI AGCGCT 1 cut(s) 275
AfiI CCNNNNNNNGG 8 cut(s) 143, 419, 470, 620, 1890, 2279, 2331, 4593
AflIII ACRYGT 2 cut(s) 3682, 4420
AgeI ACCGGT 1 cut(s) 22
AhdI GACNNNNNGTC 1 cut(s) 4555
AhlI ACTAGT 2 cut(s) 758, 4262
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 3221, 3253
AleI CACNNNNGTG 1 cut(s) 261
Alw21I GWGCWC 4 cut(s) 906, 1676, 2180, 2637
Ama87I CYCGRG 2 cut(s) 155, 867
Aor51HI AGCGCT 1 cut(s) 275
ApaI GGGCCC 1 cut(s) 4760
AseI ATTAAT 1 cut(s) 1323
AsiGI ACCGGT 1 cut(s) 22
Asp700I GAANNNNTTC 3 cut(s) 964, 1797, 3957
Asp718I GGTACC 1 cut(s) 1763
AspLEI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4913, 4993, 5110
AsuC2I CCSGG 1 cut(s) 3941
AsuII TTCGAA 4 cut(s) 2002, 2024, 3711, 3951
AvaI CYCGRG 2 cut(s) 155, 867
AvaII GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4254
BaeGI GKGCMC 4 cut(s) 1182, 4760, 4768, 5071
BaeI ACNNNNGTAYC 4 cut(s) 1747, 1780, 2742, 2775
BalI TGGCCA 2 cut(s) 1484, 1559
BamHI GGATCC 2 cut(s) 706, 2269
BanI GGYRCC 1 cut(s) 1763
BanII GRGCYC 4 cut(s) 906, 2180, 2637, 4760
BbsI GAAGAC 2 cut(s) 582, 4045
Bbv12I GWGCWC 4 cut(s) 906, 1676, 2180, 2637
BceAI ACGGC 4 cut(s) 4311, 4407, 4898, 4978
BcgI CGANNNNNNTGC 2 cut(s) 1769, 1803
BciT130I CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BciVI GTATCC 3 cut(s) 617, 1040, 2208
BclI TGATCA 3 cut(s) 753, 829, 3406
BcnI CCSGG 1 cut(s) 3941
BcuI ACTAGT 2 cut(s) 758, 4262
BfaI CTAG 8 cut(s) 488, 540, 759, 1701, 1890, 2469, 4263, 4440
BfmI CTRYAG 8 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819, 4581
BfoI RGCGCY 3 cut(s) 277, 3424, 4914
BfuI GTATCC 3 cut(s) 617, 1040, 2208
BglI GCCNNNNNGGC 1 cut(s) 4391
BglII AGATCT 1 cut(s) 3026
BmcAI AGTACT 1 cut(s) 4369
Bme18I GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4254
BmeRI GACNNNNNGTC 1 cut(s) 4555
BmeT110I CYCGRG 2 cut(s) 155, 867
BmgBI CACGTC 1 cut(s) 259
BmrI ACTGGG 4 cut(s) 1005, 1384, 3113, 4027
BmuI ACTGGG 4 cut(s) 1005, 1384, 3113, 4027
BpiI GAAGAC 2 cut(s) 582, 4045
BpmI CTGGAG 5 cut(s) 1698, 2009, 3278, 3688, 4958
Bpu14I TTCGAA 4 cut(s) 2002, 2024, 3711, 3951
BpuEI CTTGAG 4 cut(s) 600, 2311, 5009, 5087
BpuMI CCSGG 1 cut(s) 3941
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 5 cut(s) 361, 834, 1230, 2948, 3031
BsaHI GRCGYC 2 cut(s) 1093, 4551
BsaI GGTCTC 1 cut(s) 4316
BsaJI CCNNGG 9 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4316, 4434, 4760
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 8 cut(s) 143, 419, 470, 620, 1890, 2279, 2331, 4593
Bse118I RCCGGY 4 cut(s) 22, 2121, 2492, 4742
Bse3DI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4414
Bse8I GATNNNNATC 5 cut(s) 361, 834, 1230, 2948, 3031
BseBI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 9 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4316, 4434, 4760
BseGI GGATG 8 cut(s) 107, 247, 863, 1006, 1231, 2176, 3253, 4655
BseJI GATNNNNATC 5 cut(s) 361, 834, 1230, 2948, 3031
BseLI CCNNNNNNNGG 8 cut(s) 143, 419, 470, 620, 1890, 2279, 2331, 4593
BseMI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4414
BseMII CTCAG 9 cut(s) 272, 1181, 3201, 4766, 4773, 4880, 4887, 4960, 5077
BseRI GAGGAG 1 cut(s) 1079
BseSI GKGCMC 4 cut(s) 1182, 4760, 4768, 5071
BseYI CCCAGC 5 cut(s) 3975, 4356, 4461, 4610, 4640
BsgI GTGCAG 3 cut(s) 1644, 2443, 3395
Bsh1236I CGCG 2 cut(s) 3428, 3552
BshNI GGYRCC 1 cut(s) 1763
BshTI ACCGGT 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 4 cut(s) 906, 1676, 2180, 2637
BsiHKCI CYCGRG 2 cut(s) 155, 867
BsiSI CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4743
BslI CCNNNNNNNGG 8 cut(s) 143, 419, 470, 620, 1890, 2279, 2331, 4593
BsmI GAATGC 4 cut(s) 1371, 3796, 4603, 4642
Bso31I GGTCTC 1 cut(s) 4316
BsoBI CYCGRG 2 cut(s) 155, 867
Bsp119I TTCGAA 4 cut(s) 2002, 2024, 3711, 3951
Bsp120I GGGCCC 1 cut(s) 4756
Bsp1286I GDGCHC 8 cut(s) 906, 1182, 1676, 2180, 2637, 4760, 4768, 5071
Bsp1407I TGTACA 2 cut(s) 83, 1078
Bsp68I TCGCGA 1 cut(s) 3428
BspACI CCGC 9 cut(s) 704, 982, 1400, 2463, 2679, 2795, 3996, 4740, 4818
BspCNI CTCAG 9 cut(s) 273, 1182, 3200, 4765, 4774, 4879, 4888, 4959, 5076
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 2 cut(s) 3428, 3552
BspHI TCATGA 1 cut(s) 73
BspMAI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
BspT104I TTCGAA 4 cut(s) 2002, 2024, 3711, 3951
BspT107I GGYRCC 1 cut(s) 1763
BspTNI GGTCTC 1 cut(s) 4316
BsrBI CCGCTC 1 cut(s) 2463
BsrDI GCAATG 7 cut(s) 1577, 1652, 1826, 2295, 2532, 3447, 4414
BsrFI RCCGGY 4 cut(s) 22, 2121, 2492, 4742
BsrGI TGTACA 2 cut(s) 83, 1078
BssAI RCCGGY 4 cut(s) 22, 2121, 2492, 4742
BssECI CCNNGG 9 cut(s) 897, 2116, 2137, 2345, 3672, 4095, 4316, 4434, 4760
BssNI GRCGYC 2 cut(s) 1093, 4551
BssT1I CCWWGG 6 cut(s) 897, 2116, 4095, 4316, 4434, 4760
Bst2UI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
Bst6I CTCTTC 3 cut(s) 982, 2584, 3744
BstACI GRCGYC 2 cut(s) 1093, 4551
BstAPI GCANNNNNTGC 4 cut(s) 1122, 1642, 1658, 1828
BstAUI TGTACA 2 cut(s) 83, 1078
BstBI TTCGAA 4 cut(s) 2002, 2024, 3711, 3951
BstC8I GCNNGC 9 cut(s) 278, 427, 1466, 1659, 2123, 2790, 4740, 4854, 4931
BstF5I GGATG 8 cut(s) 107, 247, 863, 1006, 1231, 2176, 3253, 4655
BstFNI CGCG 2 cut(s) 3428, 3552
BstH2I RGCGCY 3 cut(s) 277, 3424, 4914
BstHHI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4913, 4993, 5110
BstNI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
BstNSI RCATGY 4 cut(s) 2705, 3501, 3686, 4424
BstSFI CTRYAG 8 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819, 4581
BstSLI GKGCMC 4 cut(s) 1182, 4760, 4768, 5071
BstUI CGCG 2 cut(s) 3428, 3552
BstV2I GAAGAC 2 cut(s) 582, 4045
BstX2I RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
BstXI CCANNNNNNTGG 5 cut(s) 4125, 4170, 4209, 4248, 4287
BstYI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
Bsu15I ATCGAT 1 cut(s) 92
BsuI GTATCC 3 cut(s) 617, 1040, 2208
BsuTUI ATCGAT 1 cut(s) 92
BtgZI GCGATG 1 cut(s) 1152
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 8 cut(s) 107, 247, 863, 1006, 1231, 2176, 3253, 4655
BtuMI TCGCGA 1 cut(s) 3428
Cac8I GCNNGC 9 cut(s) 278, 427, 1466, 1659, 2123, 2790, 4740, 4854, 4931
CciI TCATGA 1 cut(s) 73
CfoI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4913, 4993, 5110
Cfr10I RCCGGY 4 cut(s) 22, 2121, 2492, 4742
ClaI ATCGAT 1 cut(s) 92
CseI GACGC 2 cut(s) 389, 1082
CsiI ACCWGGT 1 cut(s) 469
CspAI ACCGGT 1 cut(s) 22
CspCI CAANNNNNGTGG 4 cut(s) 2463, 2498, 3511, 3546
DraI TTTAAA 1 cut(s) 2965
DraIII CACNNNGTG 1 cut(s) 1349
DrdI GACNNNNNNGTC 1 cut(s) 1313
DriI GACNNNNNGTC 1 cut(s) 4555
DseDI GACNNNNNNGTC 1 cut(s) 1313
EaeI YGGCCR 5 cut(s) 348, 1482, 1557, 2119, 4296
Eam1104I CTCTTC 3 cut(s) 982, 2584, 3744
Eam1105I GACNNNNNGTC 1 cut(s) 4555
EarI CTCTTC 3 cut(s) 982, 2584, 3744
Ecl136II GAGCTC 3 cut(s) 904, 2178, 2635
Eco130I CCWWGG 6 cut(s) 897, 2116, 4095, 4316, 4434, 4760
Eco147I AGGCCT 2 cut(s) 1195, 1252
Eco24I GRGCYC 4 cut(s) 906, 2180, 2637, 4760
Eco31I GGTCTC 1 cut(s) 4316
Eco32I GATATC 1 cut(s) 4902
Eco47I GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4254
Eco47III AGCGCT 1 cut(s) 275
Eco53kI GAGCTC 3 cut(s) 904, 2178, 2635
Eco57I CTGAAG 8 cut(s) 290, 317, 594, 1812, 3248, 4809, 4926, 5006
Eco88I CYCGRG 2 cut(s) 155, 867
EcoICRI GAGCTC 3 cut(s) 904, 2178, 2635
EcoO109I RGGNCCY 4 cut(s) 325, 1708, 4756, 4757
EcoRI GAATTC 2 cut(s) 2231, 3935
EcoRII CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
EcoRV GATATC 1 cut(s) 4902
EcoT14I CCWWGG 6 cut(s) 897, 2116, 4095, 4316, 4434, 4760
EcoT22I ATGCAT 2 cut(s) 1006, 3292
EcoT38I GRGCYC 4 cut(s) 906, 2180, 2637, 4760
ErhI CCWWGG 6 cut(s) 897, 2116, 4095, 4316, 4434, 4760
FalI AAGNNNNNCTT 6 cut(s) 915, 947, 2727, 2759, 3456, 3488
FauI CCCGC 3 cut(s) 989, 2788, 4747
FauNDI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
FbaI TGATCA 3 cut(s) 753, 829, 3406
FokI GGATG 8 cut(s) 114, 234, 850, 1013, 1238, 2183, 3260, 4662
FriOI GRGCYC 4 cut(s) 906, 2180, 2637, 4760
FspBI CTAG 8 cut(s) 488, 540, 759, 1701, 1890, 2469, 4263, 4440
GlaI GCGC 8 cut(s) 275, 1548, 3422, 3502, 3553, 4912, 4992, 5109
GsaI CCCAGC 5 cut(s) 3979, 4360, 4465, 4614, 4644
GsuI CTGGAG 5 cut(s) 1698, 2009, 3278, 3688, 4958
HaeII RGCGCY 3 cut(s) 277, 3424, 4914
HapII CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4743
HgaI GACGC 2 cut(s) 389, 1082
HhaI GCGC 8 cut(s) 276, 1549, 3423, 3503, 3554, 4913, 4993, 5110
Hin1I GRCGYC 2 cut(s) 1093, 4551
Hin6I GCGC 8 cut(s) 274, 1547, 3421, 3501, 3552, 4911, 4991, 5108
HinP1I GCGC 8 cut(s) 274, 1547, 3421, 3501, 3552, 4911, 4991, 5108
HpaII CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4743
Hpy166II GTNNAC 8 cut(s) 20, 1294, 1340, 2474, 2498, 3594, 3680, 4707
Hpy8I GTNNAC 8 cut(s) 20, 1294, 1340, 2474, 2498, 3594, 3680, 4707
Hpy99I CGWCG 3 cut(s) 2649, 3166, 4393
HpyCH4IV ACGT 3 cut(s) 258, 1296, 4551
HpySE526I ACGT 3 cut(s) 258, 1296, 4551
Hsp92I GRCGYC 2 cut(s) 1093, 4551
HspAI GCGC 8 cut(s) 274, 1547, 3421, 3501, 3552, 4911, 4991, 5108
KpnI GGTACC 1 cut(s) 1767
KroI GCCGGC 1 cut(s) 2121
KroNI GCCGGC 1 cut(s) 2123
Ksp22I TGATCA 3 cut(s) 753, 829, 3406
LmnI GCTCC 6 cut(s) 434, 901, 1420, 3707, 3830, 4920
MabI ACCWGGT 1 cut(s) 469
MaeI CTAG 8 cut(s) 488, 540, 759, 1701, 1890, 2469, 4263, 4440
MaeII ACGT 3 cut(s) 258, 1296, 4551
MaeIII GTNAC 8 cut(s) 688, 813, 892, 1392, 2095, 2853, 2954, 3882
MbiI CCGCTC 1 cut(s) 2463
MfeI CAATTG 3 cut(s) 437, 822, 1553
MflI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
MhlI GDGCHC 8 cut(s) 906, 1182, 1676, 2180, 2637, 4760, 4768, 5071
MlsI TGGCCA 2 cut(s) 1484, 1559
MluNI TGGCCA 2 cut(s) 1484, 1559
MlyI GAGTC 6 cut(s) 1904, 2060, 2344, 2807, 4177, 4216
MmeI TCCRAC 8 cut(s) 25, 156, 1280, 2247, 3060, 4015, 4574, 4671
Mox20I TGGCCA 2 cut(s) 1484, 1559
Mph1103I ATGCAT 2 cut(s) 1006, 3292
MroNI GCCGGC 1 cut(s) 2121
MroXI GAANNNNTTC 3 cut(s) 964, 1797, 3957
MscI TGGCCA 2 cut(s) 1484, 1559
MslI CAYNNNNRTG 8 cut(s) 261, 2841, 4084, 4123, 4168, 4207, 4246, 4285
Msp20I TGGCCA 2 cut(s) 1484, 1559
MspA1I CMGCKG 3 cut(s) 1444, 3434, 3623
MspI CCGG 6 cut(s) 23, 2122, 2493, 3854, 3941, 4743
MunI CAATTG 3 cut(s) 437, 822, 1553
Mva1269I GAATGC 4 cut(s) 1371, 3796, 4603, 4642
MvaI CCWGG 9 cut(s) 195, 471, 1197, 2138, 2346, 3343, 3528, 3673, 3963
MvnI CGCG 2 cut(s) 3428, 3552
NaeI GCCGGC 1 cut(s) 2123
NciI CCSGG 1 cut(s) 3941
NdeI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
NgoMIV GCCGGC 1 cut(s) 2121
NmuCI GTSAC 4 cut(s) 892, 1392, 2954, 3882
NruI TCGCGA 1 cut(s) 3428
NsiI ATGCAT 2 cut(s) 1006, 3292
NspI RCATGY 4 cut(s) 2705, 3501, 3686, 4424
NspV TTCGAA 4 cut(s) 2002, 2024, 3711, 3951
OliI CACNNNNGTG 1 cut(s) 261
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 1 cut(s) 73
PceI AGGCCT 2 cut(s) 1195, 1252
PciI ACATGT 2 cut(s) 3682, 4420
PctI GAATGC 4 cut(s) 1371, 3796, 4603, 4642
PdiI GCCGGC 1 cut(s) 2123
PdmI GAANNNNTTC 3 cut(s) 964, 1797, 3957
PflMI CCANNNNNTGG 3 cut(s) 143, 470, 4593
PfoI TCCNGGA 2 cut(s) 3939, 3961
PinAI ACCGGT 1 cut(s) 22
PleI GAGTC 6 cut(s) 1903, 2060, 2344, 2807, 4177, 4216
PpsI GAGTC 6 cut(s) 1903, 2060, 2344, 2807, 4177, 4216
PpuMI RGGWCCY 2 cut(s) 325, 1708
PscI ACATGT 2 cut(s) 3682, 4420
PshBI ATTAAT 1 cut(s) 1323
PsiI TTATAA 1 cut(s) 3537
Psp124BI GAGCTC 3 cut(s) 906, 2180, 2637
Psp5II RGGWCCY 2 cut(s) 325, 1708
Psp6I CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
PspFI CCCAGC 5 cut(s) 3975, 4356, 4461, 4610, 4640
PspGI CCWGG 9 cut(s) 193, 469, 1195, 2136, 2344, 3341, 3526, 3671, 3961
PspOMI GGGCCC 1 cut(s) 4756
PspPPI RGGWCCY 2 cut(s) 325, 1708
PstI CTGCAG 5 cut(s) 1615, 2230, 2696, 2758, 3823
PsuI RGATCY 8 cut(s) 706, 1231, 2269, 2465, 2587, 2883, 3026, 3032
PvuII CAGCTG 3 cut(s) 1444, 3434, 3623
RruI TCGCGA 1 cut(s) 3428
RseI CAYNNNNRTG 8 cut(s) 261, 2841, 4084, 4123, 4168, 4207, 4246, 4285
SacI GAGCTC 3 cut(s) 906, 2180, 2637
ScaI AGTACT 1 cut(s) 4369
SchI GAGTC 6 cut(s) 1904, 2060, 2344, 2807, 4177, 4216
SduI GDGCHC 8 cut(s) 906, 1182, 1676, 2180, 2637, 4760, 4768, 5071
SexAI ACCWGGT 1 cut(s) 469
SfcI CTRYAG 8 cut(s) 414, 1132, 1611, 2226, 2692, 2754, 3819, 4581
Sfr274I CTCGAG 1 cut(s) 155
SfuI TTCGAA 4 cut(s) 2002, 2024, 3711, 3951
SinI GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4254
SlaI CTCGAG 1 cut(s) 155
SmiMI CAYNNNNRTG 8 cut(s) 261, 2841, 4084, 4123, 4168, 4207, 4246, 4285
SmlI CTYRAG 5 cut(s) 155, 615, 2326, 5024, 5102
SmoI CTYRAG 5 cut(s) 155, 615, 2326, 5024, 5102
SpeI ACTAGT 2 cut(s) 758, 4262
SseBI AGGCCT 2 cut(s) 1195, 1252
SsiI CCGC 9 cut(s) 704, 982, 1400, 2463, 2679, 2795, 3996, 4740, 4818
SspI AATATT 2 cut(s) 1087, 1526
SspMI CTAG 8 cut(s) 488, 540, 759, 1701, 1890, 2469, 4263, 4440
SstI GAGCTC 3 cut(s) 906, 2180, 2637
StuI AGGCCT 2 cut(s) 1195, 1252
StyI CCWWGG 6 cut(s) 897, 2116, 4095, 4316, 4434, 4760
TaiI ACGT 3 cut(s) 261, 1299, 4554
TaqII GACCGA 1 cut(s) 2310
TatI WGTACW 6 cut(s) 83, 1078, 3899, 4265, 4367, 4706
TauI GCSGC 2 cut(s) 1403, 4820
TseFI GTSAC 4 cut(s) 892, 1392, 2954, 3882
Tsp45I GTSAC 4 cut(s) 892, 1392, 2954, 3882
TspGWI ACGGA 1 cut(s) 636
Van91I CCANNNNNTGG 3 cut(s) 143, 470, 4593
VpaK11BI GGWCC 7 cut(s) 325, 1340, 1708, 2322, 4176, 4215, 4254
VspI ATTAAT 1 cut(s) 1323
XceI RCATGY 4 cut(s) 2705, 3501, 3686, 4424
XcmI CCANNNNNNNNNTGG 4 cut(s) 474, 589, 1477, 4485
XhoI CTCGAG 1 cut(s) 155
XmnI GAANNNNTTC 3 cut(s) 964, 1797, 3957
XspI CTAG 8 cut(s) 488, 540, 759, 1701, 1890, 2469, 4263, 4440
ZraI GACGTC 1 cut(s) 4552
ZrmI AGTACT 1 cut(s) 4369
Zsp2I ATGCAT 2 cut(s) 1006, 3292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.