Rh1AG060100

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006658
Physical Location & Seq
Forward (+)
9831277 .. 9854645
23369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG060100.1

Sequence Viewer

Length: 5418 bp
ATGCCGCGCCAACATGCAGAAGGAGATGATTGGGGAGGACCCAAAGCAATAGTCACTCACCATTTTCAAGTTTCTTCTCTACTCAATTCCCCTTGTTCATGCAAAACCCATTTCTCCCCCTCTGATTTCAGAGAGTATCTAAAGAAAGATGGGGAGTTAGTTGGAATTAAATTTGGTTTGGCAACTCATCATGAAATTTGTACAGCATCGATGATGGGATGCTCTATTAGCCATGCCAGTCAGCTTTCCAACCCGTTTCTTGGCCTTCCTCTCGAGTTTGGGAAGTGTGAATCTTGTGGCACTTCCGAACCTGGAAAGTGCGAAGGGCATTTTGGATATATCGACTTACCATTACCGATATATCATCCTAACCACGTCAGTGAGCTGAAGCGCTTGCTGGGTTTTTTGTGCCTGAAGTGCTTAAAAATGAAAAAGAACAAGGTCCCAAGCAAGAGTGCTGGTTTGGCCGAAAGATTGTTATCTGTATGTTGTGAGGATGCTTCACAAGTTTCTATCGCAGAGATTAAACCTACAGATGGGGCTTGCTCCTTGCAATTGAAACTACCTTCTAATAAAAAACCACCGCCTGGTTTTTGGAATTTCCTCGAAAGATATGGTTTTCGTTATGGTGATGGCGTGAAACGCACTTTGCTTCCTAGAGAGGTGATGCAGATTCTCAAAAGAATCCCTGAAGACACCAGAAGAAAACTGGCTGCTAAAGGCTATAATCCTCAAGACGGATACATCTTGAATCATATACCTGTCCCTCCTAATTGCTTGTCTGTGCCTGAAATATCTGATGGTGTTACTGTTATGTCTGCGGATCCTTCAATATCAATGCTCAAAAAAGTTCTAAGGCAAGTTGAAATGATCACTAGTTCAAGGTCTGGTACAGCAAATTTTGAATCTGAAATTGTTGAGGCGAATGAGTTACAAGCAATTGTTGATCAGTATCTTCAAGTTAGGGGTACTGGAAAGGCATCCCGAGATATTGATGCAAGATTTGGAGTGACCAAGGAGCTCAATGATTCTTCAACAAAAGCATGGCTTGAAAAGATGAGGACATTGTTCATCAGAAAGGGTTCAGGTTTCTCTTCCCGCACAGTAATTACTGGGGATGCATATAGAAGGGTGAATGAAGTTGGTATCCCATATGAAATTGCTCAAAGAATAACATTCGAGGAGAAGGTCAATGTACATAATATTAGGCGTCTACAAGAGCTGGTAGATAGCAAGTTATGCCTAACCTACAGCGATGGTTCATCAACTTACTCTTTAAGGGAAGGTTCAAAGGGGCACACATTTCTGAGGCCTGGTCAAGTGGTGCATAGGAGGATAATGGATGGGGATCTTGTTTTCGTAAATAGGCCTCCAACTACCCATAAACATTCGCTTCAAGCACTTCAAGTTTACGTCCATGATGACCATGTGGTCAAGATTAATCCTCTAATATGTGGACCACTAAGTGCTGACTTTGATGGAGACTGCATTCATTTGTTCTATCCCCAGTCACTTGCGGCAAAGGCAGAAGTTATGGAGCTTTTTTCAGTGGAGAAACAGCTGCTTAGTTCTCACAGTGGCAAGCCCAATTTGCAACTGGCCACCGATTCACTTTTGTCATTAAAAATGATGTTTAAGAAATATTTTTTTGACAAAGCAGCAGCGCAGCAATTGGCCATGTTTGTTTCATCTTCATTGCCACAACCAGCTTTATTGAAAGCTAATTCTGCAGTTCCATATTGGACTGCTTTGCAGATATTGCAGACTGCATTGCCCGCTCATTTTGAGTGCTCTGGAGACAGGCACTTGGTCAGTGCTAGTGAGGTCCTCAAACTTGATTGTAATACAAGTCTTATACCAGCTCTTATCAATGACATTGGTACCTCTATTTTCTTCGAGAAAAGTGCTGAAGATGTTCTCAATTTCTTTAATTCTATGCAGCCATTGCTGATGGAGAATTTATTTTCAGAAGGGTTTAGTGTAGGTTTGGAGGATTTTGCCATGTCTAGGGAGTCCATTCAGGAGATTAGAAAGGGACTTAAAATCATTTCTCCTTTGCTGTATCATATGAGGACCACATATAATGAGCTTGTTGAGTTGCAACTGGAGAATCACATTCGAACGGTGAAACAACCAGTTTCGAATTTTATAACAAATTCATCGGCATTGGGTGATTTAATTGACTCTAAGAGTGATTCAGCCATGAACAAAGTAACTCAACAAGTTGGGTTCCTTGGCCGGCAACTTTATGACCAGGGGAAACTCTACTCAAAAACATTGGTTGAGGATGTGAGCTCTCTGTATCAGAGCAAGTATCCATCTGACATCGTGGATTATCCCTCTGCAGAATTCGGATTAGTTCATAGTGGTTTTGTTCACGGGTTGGATCCTTTTGAGGGAATGATTCATTCCATTGCTACAAGGGAAGTAATTGTTCGGTCCTCAAGAGGGTTGTCTGAACCTGGGACTCTATTCAAAAATCTAATGGCTACTCTCCGAGATGTTGTCATTTGCTATGATGGCACAGTGAGGAATGTGTGCAGTAATTCTATAATTCAGTTCGAGTATGGAGTAAAGAGCGGATCTAGTCCACAAAATTTGTTCCCTGCCGGTGAACCTGTGGGTGTGTTAGCTGCAACAGCAATGTCAAATCCAGCATACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAACTCTTCATGGGATCTGATGAAGGAAATACTGCTCTGCAAAGTCAGTTTCAAGAACGAGCTCATTGATCGTCGTGTAATCTTGTATCTGAATGACTGTGGTTGCGGAAGAAACTACTGCAGAGAACATGCGGCATATTTGGTCAAGAATCAGTTGAAAAAAGTCAGCCTTAAAGATACTGCAGTAGAGTTTATGATTGAATATCAAAATCAGCGAGCTGCGGGAAGCATGGAAATTGACTCTGGCCTTGTAGGTCATGTTCATTTGAATGAGATGCTGTTACGGGAGTTAAATCTGGGAATGTATGAGATCCTTCAAAAATGCGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTTGGGAAGAAGATGAACATCGGTGACATTTTTAAAAGGACAGTTTTATCGTACAGTGACTGTTGTTCTTTTCATCAATCCTCTGCTGAAAATAGATCTGGATCTCCTTGCTTGATGTTCTTCTACCAAGATTTTAATAATTCAGAGTTGGAGGCCATTTCACAAATCTTGGCTGACTTTATTTGCCCAGTTCTTCTAAAAACAGTAATTAAAGGTGATCCTCGAATTTCGTCGGCGAACATAATTTGGATCAACTCAGAATCAACAACTTGGATAAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCATGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATGTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAAGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCGACAGCTGTTGCAATGGTGGCGAAAGGTGTTCTTAAAGAGCATCTTATTCTCTTGGCAAACAGTATGACATGCGCTGGAAATTTCGTTGGTTTCAATCCTGGTGGTTATAAAGCACTATCTCGCGCATTGAATATACAAGTACCCTTTACAGAAGCAACATTGTTTACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCTTTGTCGAGCATAGTTGCATCATGTTCCTGGGGTAAACATGTAGCTGTTGGTACTGGCTCCAGATTCGAAATCCTCTGGGACACAAGAGAGGGAGGTTTGAACGAAGAGGGTGGGATAGATGTTTTCAACTTTCTACATATGGTGAATGCTGCAAATGGAGAAGAATCAACTACTGCAGCTTTGGGAGCAGAAATTGATGATCTTGTGCCGGAATATGAAATTGGAGATGTGTCCTTGTCACCAGAACATAACTGTACTTCTGATAAGCCAGTGTTTGAAGATATTGTTGAATTCCCGGATAGTTTCGAAAATGTTCCTGGAAAATCAAGCTGGGATTCATCTCTCAACACCGCTTCTTCTGGTGGGAAAGACTGGGGGGCAGTTAAAGTTGGAAGACAAGATAGCATTTCAGCAGAAACCCAAGTGGATAGCACATCTGCATGGGGGGCCAAGGCTAGTACAGAAGATGCCCCATCTGCATGGGGAACAACCAAGACTGCTACTAAAGAAGATGCCCCATCTGTATGGGGGACCAAGACTCGTACAGAAGAAGCCCCATCTGCATGGGGGACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAACCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGAAGCCCCATCTGCATGGGGTACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAACCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGAAGCCCCATCTGCATGGGGTACCAAGACTAGTACAGAAGAAGCCTCATCTGCATGGGGTACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAACCAAGACTGCTACTAAAGAAGATGCCCCATCTGCATGGGGGACCAAGACTCGTACAGAAGAAGCCCCATCTGCATGGGGTACCAAGACTAGTACAGAAGATGCCCCATCTGCATGGGGAACAGCCAAGACTGCTACATCTCCATGGGAGACCAACACTGGTAGAGAAGATTCCACATCGGGCTGGGGGACAAGTACTGCCAGAGAAGATGCCACGACGGCATGGGGGAAAAGCAATGAAAAGAAACATGTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTTGCTGGGGGACAGAAAATCCACAATCTACATGGAGGACAGAAAGCGAGAATGGTTGGTCTGGTGGTGGGGTGAGTAAAGCTGAATCAACAGATGTCCAGTCCAAGAAAGCACAAGAAGATTCTACATCCAATGGTTGGAATGCCTTTTCTGCTGGGCCAACAAAACCCCAAAAGGAGAATGCTGGGTCTGGATGGTGCATGAAAGGTTTTGAGAAGAAAGATGGTAATGAGTTGGAACAAGATAAGTCTACTGCCCGTCATTCTTGGGGGCAGCAAAAGCCCGCCGGTACTTCAGAAGGTGCTTGGGGTCAGGAAAAGCCTGCCGATACTTCTCAGGGCAGTTGGGGACGGCAAAAGTCTCCTGATATTTCTGAAGGCGCATGGGGGCAGCAAAAGCCTGATACAACTTCTCAAGGCTCTTGGGGACAGCAAAAGACTCCTGAAATGTCTCAGGTGATGATAAATAACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1805

Amino Acids

197.95

Weight (kDa)

6.03

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 87 - 320 3.4e-10 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 363 - 517 4.8e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 520 - 665 5.9e-10 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 794 - 1220 1.4e-09 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2150, 3654
AasI GACNNNNNNGTC 1 cut(s) 1430
Acc65I GGTACC 5 cut(s) 1880, 4454, 4577, 4616, 4739
AccB1I GGYRCC 5 cut(s) 1880, 4454, 4577, 4616, 4739
AccB7I CCANNNNNTGG 2 cut(s) 587, 5079
AccBSI CCGCTC 2 cut(s) 1778, 2580
AccI GTMKAC 2 cut(s) 1213, 5192
AccII CGCG 3 cut(s) 7, 3545, 3669
AcoI YGGCCR 4 cut(s) 465, 1599, 1674, 2236
AcuI CTGAAG 7 cut(s) 407, 434, 711, 1929, 3365, 5220, 5337
AcyI GRCGYC 1 cut(s) 1210
AdeI CACNNNGTG 1 cut(s) 1466
AfeI AGCGCT 1 cut(s) 392
AfiI CCNNNNNNNGG 8 cut(s) 260, 536, 587, 737, 2007, 2396, 2448, 5079
AflIII ACRYGT 2 cut(s) 3799, 4906
AhlI ACTAGT 6 cut(s) 875, 4340, 4463, 4586, 4625, 4748
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 9 cut(s) 310, 586, 1312, 2253, 2461, 3458, 3643, 3788, 4078
AjuI GAANNNNNNNTTGG 6 cut(s) 315, 347, 3338, 3370, 3966, 3998
AleI CACNNNNGTG 1 cut(s) 378
Alw21I GWGCWC 4 cut(s) 1023, 1793, 2297, 2754
Alw26I GTCTC 5 cut(s) 1476, 1791, 4802, 5307, 5397
AlwNI CAGNNNCTG 1 cut(s) 3108
Ama87I CYCGRG 2 cut(s) 272, 984
Aor51HI AGCGCT 1 cut(s) 392
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AseI ATTAAT 1 cut(s) 1440
Asp700I GAANNNNTTC 3 cut(s) 1081, 1914, 4074
Asp718I GGTACC 5 cut(s) 1880, 4454, 4577, 4616, 4739
AspLEI GCGC 7 cut(s) 9, 393, 1666, 3540, 3620, 3671, 5324
AsuC2I CCSGG 1 cut(s) 4058
AsuII TTCGAA 4 cut(s) 2119, 2141, 3828, 4068
AvaI CYCGRG 2 cut(s) 272, 984
BaeGI GKGCMC 1 cut(s) 1299
BaeI ACNNNNGTAYC 4 cut(s) 1864, 1897, 2859, 2892
BalI TGGCCA 2 cut(s) 1601, 1676
BamHI GGATCC 2 cut(s) 823, 2386
BanI GGYRCC 5 cut(s) 1880, 4454, 4577, 4616, 4739
BanII GRGCYC 3 cut(s) 1023, 2297, 2754
BbsI GAAGAC 2 cut(s) 699, 4162
Bbv12I GWGCWC 4 cut(s) 1023, 1793, 2297, 2754
BceAI ACGGC 2 cut(s) 4893, 5309
BcgI CGANNNNNNTGC 2 cut(s) 1886, 1920
BciT130I CCWGG 9 cut(s) 312, 588, 1314, 2255, 2463, 3460, 3645, 3790, 4080
BciVI GTATCC 3 cut(s) 734, 1157, 2325
BclI TGATCA 3 cut(s) 870, 946, 3523
BcnI CCSGG 1 cut(s) 4058
BcoDI GTCTC 5 cut(s) 1476, 1791, 4802, 5307, 5397
BcuI ACTAGT 6 cut(s) 875, 4340, 4463, 4586, 4625, 4748
BfmI CTRYAG 7 cut(s) 531, 1249, 1728, 2343, 2809, 2871, 3936
BfoI RGCGCY 2 cut(s) 394, 3541
BfuI GTATCC 3 cut(s) 734, 1157, 2325
BglI GCCNNNNNGGC 1 cut(s) 4877
BglII AGATCT 1 cut(s) 3143
BmcAI AGTACT 1 cut(s) 4855
BmeT110I CYCGRG 2 cut(s) 272, 984
BmgBI CACGTC 1 cut(s) 376
BmrI ACTGGG 4 cut(s) 1122, 1501, 3230, 4144
BmuI ACTGGG 4 cut(s) 1122, 1501, 3230, 4144
BpiI GAAGAC 2 cut(s) 699, 4162
BpmI CTGGAG 4 cut(s) 1815, 2126, 3395, 3805
Bpu14I TTCGAA 4 cut(s) 2119, 2141, 3828, 4068
BpuEI CTTGAG 3 cut(s) 717, 2428, 5340
BpuMI CCSGG 1 cut(s) 4058
Bsa29I ATCGAT 1 cut(s) 209
BsaBI GATNNNNATC 5 cut(s) 478, 951, 1347, 3065, 3148
BsaHI GRCGYC 1 cut(s) 1210
BsaI GGTCTC 1 cut(s) 4802
BsaJI CCNNGG 8 cut(s) 1014, 2233, 2254, 2462, 3789, 4212, 4802, 4920
Bsc4I CCNNNNNNNGG 8 cut(s) 260, 536, 587, 737, 2007, 2396, 2448, 5079
Bse118I RCCGGY 3 cut(s) 2238, 2609, 5228
Bse3DI GCAATG 7 cut(s) 1694, 1769, 1943, 2412, 2649, 3564, 4900
Bse8I GATNNNNATC 5 cut(s) 478, 951, 1347, 3065, 3148
BseBI CCWGG 9 cut(s) 312, 588, 1314, 2255, 2463, 3460, 3645, 3790, 4080
BseCI ATCGAT 1 cut(s) 209
BseDI CCNNGG 8 cut(s) 1014, 2233, 2254, 2462, 3789, 4212, 4802, 4920
BseJI GATNNNNATC 5 cut(s) 478, 951, 1347, 3065, 3148
BseLI CCNNNNNNNGG 8 cut(s) 260, 536, 587, 737, 2007, 2396, 2448, 5079
BseMI GCAATG 7 cut(s) 1694, 1769, 1943, 2412, 2649, 3564, 4900
BseMII CTCAG 4 cut(s) 1298, 3318, 5291, 5408
BseRI GAGGAG 1 cut(s) 1196
BseSI GKGCMC 1 cut(s) 1299
BseYI CCCAGC 6 cut(s) 397, 4092, 4842, 4947, 5096, 5126
BsgI GTGCAG 2 cut(s) 2560, 3512
Bsh1236I CGCG 3 cut(s) 7, 3545, 3669
BshNI GGYRCC 5 cut(s) 1880, 4454, 4577, 4616, 4739
BshVI ATCGAT 1 cut(s) 209
BsiHKAI GWGCWC 4 cut(s) 1023, 1793, 2297, 2754
BsiHKCI CYCGRG 2 cut(s) 272, 984
BsiSI CCGG 5 cut(s) 2239, 2610, 3971, 4058, 5229
BslI CCNNNNNNNGG 8 cut(s) 260, 536, 587, 737, 2007, 2396, 2448, 5079
BsmAI GTCTC 5 cut(s) 1476, 1791, 4802, 5307, 5397
BsmI GAATGC 4 cut(s) 1488, 3913, 5089, 5128
Bso31I GGTCTC 1 cut(s) 4802
BsoBI CYCGRG 2 cut(s) 272, 984
Bsp119I TTCGAA 4 cut(s) 2119, 2141, 3828, 4068
Bsp1286I GDGCHC 5 cut(s) 1023, 1299, 1793, 2297, 2754
Bsp1407I TGTACA 2 cut(s) 200, 1195
Bsp19I CCATGG 1 cut(s) 4802
Bsp68I TCGCGA 1 cut(s) 3545
BspCNI CTCAG 4 cut(s) 1299, 3317, 5290, 5407
BspDI ATCGAT 1 cut(s) 209
BspFNI CGCG 3 cut(s) 7, 3545, 3669
BspHI TCATGA 1 cut(s) 190
BspMAI CTGCAG 5 cut(s) 1732, 2347, 2813, 2875, 3940
BspT104I TTCGAA 4 cut(s) 2119, 2141, 3828, 4068
BspT107I GGYRCC 5 cut(s) 1880, 4454, 4577, 4616, 4739
BspTNI GGTCTC 1 cut(s) 4802
BsrBI CCGCTC 2 cut(s) 1778, 2580
BsrDI GCAATG 7 cut(s) 1694, 1769, 1943, 2412, 2649, 3564, 4900
BsrFI RCCGGY 3 cut(s) 2238, 2609, 5228
BsrGI TGTACA 2 cut(s) 200, 1195
BssAI RCCGGY 3 cut(s) 2238, 2609, 5228
BssECI CCNNGG 8 cut(s) 1014, 2233, 2254, 2462, 3789, 4212, 4802, 4920
BssNI GRCGYC 1 cut(s) 1210
BssT1I CCWWGG 5 cut(s) 1014, 2233, 4212, 4802, 4920
Bst2UI CCWGG 9 cut(s) 312, 588, 1314, 2255, 2463, 3460, 3645, 3790, 4080
Bst6I CTCTTC 3 cut(s) 1099, 2701, 3861
BstACI GRCGYC 1 cut(s) 1210
BstAPI GCANNNNNTGC 3 cut(s) 1239, 1759, 1945
BstAUI TGTACA 2 cut(s) 200, 1195
BstBI TTCGAA 4 cut(s) 2119, 2141, 3828, 4068
BstC8I GCNNGC 8 cut(s) 395, 544, 1583, 1776, 2240, 2907, 5226, 5265
BstDEI CTNAG 8 cut(s) 854, 1307, 1463, 1565, 2187, 3304, 5277, 5394
BstDSI CCRYGG 1 cut(s) 4802
BstFNI CGCG 3 cut(s) 7, 3545, 3669
BstH2I RGCGCY 2 cut(s) 394, 3541
BstHHI GCGC 7 cut(s) 9, 393, 1666, 3540, 3620, 3671, 5324
BstMAI GTCTC 5 cut(s) 1476, 1791, 4802, 5307, 5397
BstNI CCWGG 9 cut(s) 312, 588, 1314, 2255, 2463, 3460, 3645, 3790, 4080
BstNSI RCATGY 5 cut(s) 17, 2822, 3618, 3803, 4910
BstSFI CTRYAG 7 cut(s) 531, 1249, 1728, 2343, 2809, 2871, 3936
BstSLI GKGCMC 1 cut(s) 1299
BstUI CGCG 3 cut(s) 7, 3545, 3669
BstV2I GAAGAC 2 cut(s) 699, 4162
BstX2I RGATCY 8 cut(s) 823, 1348, 2386, 2582, 2704, 3000, 3143, 3149
BstYI RGATCY 8 cut(s) 823, 1348, 2386, 2582, 2704, 3000, 3143, 3149
Bsu15I ATCGAT 1 cut(s) 209
BsuI GTATCC 3 cut(s) 734, 1157, 2325
BsuTUI ATCGAT 1 cut(s) 209
BtgI CCRYGG 1 cut(s) 4802
BtgZI GCGATG 1 cut(s) 1269
BtrI CACGTC 1 cut(s) 376
BtsIMutI CAGTG 9 cut(s) 385, 1554, 1582, 1819, 2532, 3109, 3444, 4038, 4815
BtuMI TCGCGA 1 cut(s) 3545
Cac8I GCNNGC 8 cut(s) 395, 544, 1583, 1776, 2240, 2907, 5226, 5265
CaiI CAGNNNCTG 1 cut(s) 3108
CciI TCATGA 1 cut(s) 190
CfoI GCGC 7 cut(s) 9, 393, 1666, 3540, 3620, 3671, 5324
Cfr10I RCCGGY 3 cut(s) 2238, 2609, 5228
ClaI ATCGAT 1 cut(s) 209
CseI GACGC 1 cut(s) 1199
CspCI CAANNNNNGTGG 4 cut(s) 2580, 2615, 3628, 3663
DdeI CTNAG 8 cut(s) 854, 1307, 1463, 1565, 2187, 3304, 5277, 5394
DraI TTTAAA 1 cut(s) 3082
DraIII CACNNNGTG 1 cut(s) 1466
DrdI GACNNNNNNGTC 1 cut(s) 1430
DseDI GACNNNNNNGTC 1 cut(s) 1430
EaeI YGGCCR 4 cut(s) 465, 1599, 1674, 2236
Eam1104I CTCTTC 3 cut(s) 1099, 2701, 3861
EarI CTCTTC 3 cut(s) 1099, 2701, 3861
Ecl136II GAGCTC 3 cut(s) 1021, 2295, 2752
Eco130I CCWWGG 5 cut(s) 1014, 2233, 4212, 4802, 4920
Eco147I AGGCCT 2 cut(s) 1312, 1369
Eco24I GRGCYC 3 cut(s) 1023, 2297, 2754
Eco31I GGTCTC 1 cut(s) 4802
Eco47III AGCGCT 1 cut(s) 392
Eco53kI GAGCTC 3 cut(s) 1021, 2295, 2752
Eco57I CTGAAG 7 cut(s) 407, 434, 711, 1929, 3365, 5220, 5337
Eco88I CYCGRG 2 cut(s) 272, 984
EcoICRI GAGCTC 3 cut(s) 1021, 2295, 2752
EcoO109I RGGNCCY 3 cut(s) 38, 442, 1825
EcoRI GAATTC 2 cut(s) 2348, 4052
EcoRII CCWGG 9 cut(s) 310, 586, 1312, 2253, 2461, 3458, 3643, 3788, 4078
EcoT14I CCWWGG 5 cut(s) 1014, 2233, 4212, 4802, 4920
EcoT22I ATGCAT 2 cut(s) 1123, 3409
EcoT38I GRGCYC 3 cut(s) 1023, 2297, 2754
ErhI CCWWGG 5 cut(s) 1014, 2233, 4212, 4802, 4920
FalI AAGNNNNNCTT 8 cut(s) 1032, 1064, 2844, 2876, 3561, 3593, 3573, 3605
FauI CCCGC 4 cut(s) 1106, 1783, 2905, 5233
FauNDI CATATG 5 cut(s) 1153, 2067, 3475, 3753, 3900
FbaI TGATCA 3 cut(s) 870, 946, 3523
FblI GTMKAC 2 cut(s) 1213, 5192
FriOI GRGCYC 3 cut(s) 1023, 2297, 2754
GlaI GCGC 7 cut(s) 8, 392, 1665, 3539, 3619, 3670, 5323
GsaI CCCAGC 6 cut(s) 401, 4096, 4846, 4951, 5100, 5130
GsuI CTGGAG 4 cut(s) 1815, 2126, 3395, 3805
HaeII RGCGCY 2 cut(s) 394, 3541
HapII CCGG 5 cut(s) 2239, 2610, 3971, 4058, 5229
HgaI GACGC 1 cut(s) 1199
HhaI GCGC 7 cut(s) 9, 393, 1666, 3540, 3620, 3671, 5324
Hin1I GRCGYC 1 cut(s) 1210
Hin6I GCGC 7 cut(s) 7, 391, 1664, 3538, 3618, 3669, 5322
HinP1I GCGC 7 cut(s) 7, 391, 1664, 3538, 3618, 3669, 5322
HpaII CCGG 5 cut(s) 2239, 2610, 3971, 4058, 5229
Hpy166II GTNNAC 9 cut(s) 1214, 1411, 1457, 2377, 2591, 2615, 3711, 3797, 5193
Hpy8I GTNNAC 9 cut(s) 1214, 1411, 1457, 2377, 2591, 2615, 3711, 3797, 5193
Hpy99I CGWCG 3 cut(s) 2766, 3283, 4879
HpyCH4IV ACGT 2 cut(s) 375, 1413
HpyF3I CTNAG 8 cut(s) 854, 1307, 1463, 1565, 2187, 3304, 5277, 5394
HpySE526I ACGT 2 cut(s) 375, 1413
Hsp92I GRCGYC 1 cut(s) 1210
HspAI GCGC 7 cut(s) 7, 391, 1664, 3538, 3618, 3669, 5322
KpnI GGTACC 5 cut(s) 1884, 4458, 4581, 4620, 4743
KroI GCCGGC 1 cut(s) 2238
KroNI GCCGGC 1 cut(s) 2240
Ksp22I TGATCA 3 cut(s) 870, 946, 3523
LmnI GCTCC 5 cut(s) 551, 1018, 1537, 3824, 3947
MaeII ACGT 2 cut(s) 375, 1413
MbiI CCGCTC 2 cut(s) 1778, 2580
MfeI CAATTG 3 cut(s) 554, 939, 1670
MflI RGATCY 8 cut(s) 823, 1348, 2386, 2582, 2704, 3000, 3143, 3149
MhlI GDGCHC 5 cut(s) 1023, 1299, 1793, 2297, 2754
MlsI TGGCCA 2 cut(s) 1601, 1676
MluNI TGGCCA 2 cut(s) 1601, 1676
MlyI GAGTC 9 cut(s) 2021, 2177, 2461, 2924, 4294, 4417, 4540, 4702, 5374
MmeI TCCRAC 8 cut(s) 142, 273, 1397, 2364, 3177, 4132, 5060, 5157
Mox20I TGGCCA 2 cut(s) 1601, 1676
Mph1103I ATGCAT 2 cut(s) 1123, 3409
MroNI GCCGGC 1 cut(s) 2238
MroXI GAANNNNTTC 3 cut(s) 1081, 1914, 4074
MscI TGGCCA 2 cut(s) 1601, 1676
Msp20I TGGCCA 2 cut(s) 1601, 1676
MspA1I CMGCKG 3 cut(s) 1561, 3551, 3740
MspI CCGG 5 cut(s) 2239, 2610, 3971, 4058, 5229
MunI CAATTG 3 cut(s) 554, 939, 1670
Mva1269I GAATGC 4 cut(s) 1488, 3913, 5089, 5128
MvaI CCWGG 9 cut(s) 312, 588, 1314, 2255, 2463, 3460, 3645, 3790, 4080
MvnI CGCG 3 cut(s) 7, 3545, 3669
NaeI GCCGGC 1 cut(s) 2240
NciI CCSGG 1 cut(s) 4058
NcoI CCATGG 1 cut(s) 4802
NdeI CATATG 5 cut(s) 1153, 2067, 3475, 3753, 3900
NgoMIV GCCGGC 1 cut(s) 2238
NmuCI GTSAC 6 cut(s) 52, 1009, 1509, 3071, 3104, 3999
NruI TCGCGA 1 cut(s) 3545
NsiI ATGCAT 2 cut(s) 1123, 3409
NspI RCATGY 5 cut(s) 17, 2822, 3618, 3803, 4910
NspV TTCGAA 4 cut(s) 2119, 2141, 3828, 4068
OliI CACNNNNGTG 1 cut(s) 378
PaeR7I CTCGAG 1 cut(s) 272
PagI TCATGA 1 cut(s) 190
PceI AGGCCT 2 cut(s) 1312, 1369
PciI ACATGT 2 cut(s) 3799, 4906
PctI GAATGC 4 cut(s) 1488, 3913, 5089, 5128
PdiI GCCGGC 1 cut(s) 2240
PdmI GAANNNNTTC 3 cut(s) 1081, 1914, 4074
PflMI CCANNNNNTGG 2 cut(s) 587, 5079
PfoI TCCNGGA 2 cut(s) 4056, 4078
PleI GAGTC 9 cut(s) 2020, 2177, 2461, 2924, 4294, 4417, 4540, 4702, 5374
PpsI GAGTC 9 cut(s) 2020, 2177, 2461, 2924, 4294, 4417, 4540, 4702, 5374
PpuMI RGGWCCY 3 cut(s) 38, 442, 1825
PscI ACATGT 2 cut(s) 3799, 4906
PshBI ATTAAT 1 cut(s) 1440
PsiI TTATAA 2 cut(s) 2150, 3654
Psp124BI GAGCTC 3 cut(s) 1023, 2297, 2754
Psp5II RGGWCCY 3 cut(s) 38, 442, 1825
Psp6I CCWGG 9 cut(s) 310, 586, 1312, 2253, 2461, 3458, 3643, 3788, 4078
PspFI CCCAGC 6 cut(s) 397, 4092, 4842, 4947, 5096, 5126
PspGI CCWGG 9 cut(s) 310, 586, 1312, 2253, 2461, 3458, 3643, 3788, 4078
PspPPI RGGWCCY 3 cut(s) 38, 442, 1825
PsrI GAACNNNNNNTAC 2 cut(s) 2934, 2966
PstI CTGCAG 5 cut(s) 1732, 2347, 2813, 2875, 3940
PstNI CAGNNNCTG 1 cut(s) 3108
PsuI RGATCY 8 cut(s) 823, 1348, 2386, 2582, 2704, 3000, 3143, 3149
PvuII CAGCTG 3 cut(s) 1561, 3551, 3740
RruI TCGCGA 1 cut(s) 3545
SacI GAGCTC 3 cut(s) 1023, 2297, 2754
ScaI AGTACT 1 cut(s) 4855
SchI GAGTC 9 cut(s) 2021, 2177, 2461, 2924, 4294, 4417, 4540, 4702, 5374
SduI GDGCHC 5 cut(s) 1023, 1299, 1793, 2297, 2754
SfcI CTRYAG 7 cut(s) 531, 1249, 1728, 2343, 2809, 2871, 3936
Sfr274I CTCGAG 1 cut(s) 272
SfuI TTCGAA 4 cut(s) 2119, 2141, 3828, 4068
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 4 cut(s) 272, 732, 2443, 5355
SmoI CTYRAG 4 cut(s) 272, 732, 2443, 5355
SpeI ACTAGT 6 cut(s) 875, 4340, 4463, 4586, 4625, 4748
SseBI AGGCCT 2 cut(s) 1312, 1369
SspI AATATT 2 cut(s) 1204, 1643
SstI GAGCTC 3 cut(s) 1023, 2297, 2754
StuI AGGCCT 2 cut(s) 1312, 1369
StyI CCWWGG 5 cut(s) 1014, 2233, 4212, 4802, 4920
TaiI ACGT 2 cut(s) 378, 1416
TaqII GACCGA 1 cut(s) 2427
TauI GCSGC 3 cut(s) 7, 1520, 2825
TscAI CASTG 9 cut(s) 385, 1554, 1582, 1819, 2532, 3109, 3444, 4038, 4822
TseFI GTSAC 6 cut(s) 52, 1009, 1509, 3071, 3104, 3999
Tsp45I GTSAC 6 cut(s) 52, 1009, 1509, 3071, 3104, 3999
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 9 cut(s) 385, 1554, 1582, 1819, 2532, 3109, 3444, 4038, 4822
Van91I CCANNNNNTGG 2 cut(s) 587, 5079
VspI ATTAAT 1 cut(s) 1440
XceI RCATGY 5 cut(s) 17, 2822, 3618, 3803, 4910
XcmI CCANNNNNNNNNTGG 3 cut(s) 706, 1594, 4971
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 2 cut(s) 1213, 5192
XmnI GAANNNNTTC 3 cut(s) 1081, 1914, 4074
ZrmI AGTACT 1 cut(s) 4855
Zsp2I ATGCAT 2 cut(s) 1123, 3409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.