MD07G1039700.v1.1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006658
Physical Location & Seq
Forward (+)
3360512 .. 3376150
15639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1039700.v1.1.491

Sequence Viewer

Length: 5286 bp
ATGGAGGAGGCCTCGACCTCAAATATTTTAGATGGGGAAATCGTTGGAATCAAATTTGGTTTAGCAACTCATCAAGAAATTTGCACAGCATCTATTAGTGATTGCTCTATCAGCCATGCAAGTCAACTTTCCAACCCCTTCCTTGGCCTGCCCCTTGAGTTCGGAAAGTGTGAATCTTGTGGCACTTCTGAACCTGGGAAGTGTGAAGGTCATTTTGGGTATATTGAGTTACCAGTACCAATATTTCATCCTAATCATGTTAGCGAATTGAAGCGGATGCTGAGCTTATTGTGCTTGAAATGCTTGAAAATGAAAAAGAACAAGTTTCCAACCAAGAATGCTGGCTTAGCAGAAAGAATGTTAGCTTCATGTTGTGAGGATGCTGCACAAGTGTCTATTGGAGAGACTAAACCAACAGATAGCTCTTGTTCCTTGCAATTGAAGCGACCTTCTAAGTCAAGAACACCACCTGGTTTTTGGAATTTCCTAGAACGATATGGTTTTCGTTATGGTGATGATCATATACGGACTTTGCTTCCTTCTGAGGTGCTGGAGATGCTAAAGCGAATCCCTCAAGACACCAGGAGGAAACTTGCTGCAAAAGGCTATTTTCCTCAAGATGGATACATCTTGCGTCATATACCTGTCCCTCCGAACTGTTTATCCGTGCCTGAGATTTCAGATGGTGTTAGCGTTATGTCCGCGGATCCTTCGATTTCAATGCTTAGAAAAGTTCTAAAGCAAATTGAAGTCATCAGAAGTTCAAGGTCGGGCATACCCAATTTTGAATCCCAAATTGTAGAAGCCAATGAACTTCAAGCAATTGTAGATCAGTATCTTCAAGTTAGGGGTACTGGGAAGGCATCACGAGACATTGATGCGAGATTAGGGGTTAATAGGGAGCTTAATGCCTCTTCAACGAAAGCTTGGCTTGAAAAAATGAGGACACTGTTCATCAGAAAGGGTTCAGGCTTCTCTTCCCGCTCAGTGATTACGGGGGATGCATATAAAAGGGTGAATGAAGTTGGCATCCCTTATGAAATTGCTCAAAGAATCACCTTTGAGGAGAAGGTTAATGACCATAACATTAGGTACCTACAAGAGCTAGTAGATAAGAAGTTGTGCCTAACCTATAGGGATGGTTCATCAACTTACTCATTAAGGGAAGGTTCCAAAGGGCACACATTTTTGAGGCCTGGTCAAGTGGTGCATCGGAGGATTCTGGACGGGGACCTTGTATTCGTAAACAGGCCCCCAACCACACACAAACATTCACTACAAGCACTACAAGTGTATGTGCATGATGACCATGTGGTGAAAATAAATCCTCTGATATGTGGGCCACTAAGTGCTGACTTTGATGGTGATTGCATCCATTTGTTCTATCCGCAATCTCTTGCTGCAAAGGCAGAAGTTTTGGAGCTTTTTTCAGTGGAGAAGCAGCTGCTTAGCTCTCATAGTGGAAAGCCAAATTTGCAAATGGCCACTGATTCACTGTTGTCATTGAAGATGATGTTTAAAAACTATTTTTTGGATAAATCAGCAGCTCAGCAACTGGCCATGTTTGCTTCATCTTCCTTGCCACGACCTGCATTATTGAAAGCAAATGCTGCTCGCTCATATTGGACTGCTTTGCAGATATTACAGACTGCATTGCCTGCTCACTTTGATTACTGTGGGGAAAAGTACTTAGTCAGTAAAAGTGAGATACTGAAAATTGATTTCAATACAAGTGAAGTTTCAGCAGTGATGAATGACGTTGCTACCTCAGTTTTCTTCGTTAAAGGTGGTGAAGATGTCCTCAAATTCTTTGATTCTCTACAGCCCTTGCTGATGGAGAACATATTTTCAGAAGGGTTCAGCGTTGGCTTGGAAGATTTTTACATGTCAAAGACATCAATACAAGATATTCAGAAGAATATTCAGGATACTTCAGATTTGTTGTATCATCTGAGGTCAACCTACAATGAATTTGTAGAGTTTCAGTTAGAGAGTCGAATCAAAAACTTGAAAGTACCAGTTTCAAGTTTTATCTTGGAGTCATCTGCATTTGGTGATTTAATCGACTCCAAGAGTGATTCGGCAATCAACAAAGTTGTACAACAAATTGGGTTCCTAGGCCTACAACTCTCTGATAAGGGAAGATTCTACTCGAAGACGTTAGTTGAGGATGTGGCCTCTCTCTATCACAACAAGTATTCTTTTAACATGGAGTATCCCTCTGCAGAATATGGACTAGTTCAAAGCTGTTTCTTCCATGGGTTAGATCCATACGAGGAGATAGTTCACTCCATTGCTACACGAGAGGTGATTGTTCGGTCCTCAAGAGGATTGACCGAACCTGGGACCTTATTTAAAAATTTAATGGCCATTCTTCGGGATGTGGTTATTTGCTATGATGGCACGGTCAGAAATGTATGTAGCAATTCAGTAATCCAATTTGAGTATGGAGTCAACGTTGGATCTAGGCCCCCGAATCTGTTCCCTGCTGGTGAACCTGTTGGTGTGTTAGCTGCCACTGCAATGTCAAATCCTGCGTACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAATTCTTCATGGGAGCTGATGAAGGAAATTTTGCTTTGCAAAGTCAATTTTAAGAATGAGCTCATTGATCGCCGTGTAATTTTGTACTTGAGTAACTGTGGTTGTGGAAGAACATTCTGCCGAGAACGTGCAGCATGTTTGGTTAAGAATCAGTTGAAGAAAGTCAGTCTTAAAGATACTGCAGTTGAATTCATGATTGAATATAGCAATCAGCTTTCTAGTTTGGGAAGTATGGTAAATGATGCAGGCCTGGTTGGTCATATTCATCTTAAAGAGGAGCTGTTAAGGGAATTAAATGTTGGTGTAGACGAGATTCGACAAAAATGCCAAGAAACTGTTAATTCATTTCGGAGGAAGAGAGTTGGGAAGAAGAAGTTCAACATTGGCTATCTTTTCAAAAATACAGTTTTGTTTGCCAGTGAGCATTGCTCGTTTCATCATTCCTGTGCAGACAAAAGATCTGACTCCCCATGCTTGATGTTCTTCCTTCAAGCTACTGAAGAGTTGGAGACCACTTCGCAATACTATGCTGACTTGATTTGCCCTGTGCTTTTAGAAACAATAATAAAAGGTGACCCTCGAATTTCGTCGGCAAATATAATATGGATTGATCCAGATACAACAACTTGGATTAGAAGCCCAAATAAATCTCAAAAGGGTGAATGGGCTTTGGATGTTGTTCTGGAGAAATCTGTTGTCAAGCAAAGCGGTGATGCATGGAGAATCGTTCTCGATTCTTGCCTCCCCGTGCTTCACCTAATTGACACCAGACGTTCCATCCCATATGCCATCAAACAGATTCAAGAGCTGTTGGGAGTGTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTCGCAACAGCTGTTACAATGGTGGCGAAAGGTGTCCTGAAAGAGCATCTTATCCTCCTGGCAAATAGCATGACATGTGCTGGAAATTTTGTTGGCTTTAATTCTAGTGGATATAAGGCACTATCTCGCGCATTGAATATTCAAGTACCATTTACAGAAGCAACACTTTTTACACCAAGAAAGTGCTTTGAGAGAGCAGCTGAAAAGTGCCACATGGATTCTTTGTCAAGCATAGTTGCATCATGTTCATGGGGTAAACATGTTGCTGTTGGTACTGGATCCAGGTTTGATATCCTGTGGGACACACGAGAGGGAGGATTGAATCAAGAGGGTGGATTAGATGTTTTCAACTTTCTGCATATGGTGAGCACAGCAAATGGAGAAGAAGCAACTACGGGAGCACTGGGGGCAGAAGTTGATGATCTTATGGTGGTAGATGAATTAGCAGATTCGTGCTTATCGCCAGAACTTGACTCTGGTCTAGATAGACCCGTCTTTGAAGATATTATTGAATTTGAGGACAAATCAGAAACTCCTCCTAGAAAATCAAGTTGGGAAAATGATTCCTCTGTATGGGGGACAAATACTACAAGAGAAGATGCCACATCATCTTGGGGGAAAACTCCAGCTAGAGAAGCTACCACATCTACTTGGGGGATAGATAAGGCAGCAGAAGATACCACTCCCGCATGGGGGACAAATACTGCCAGGGAAGGTGCGACATCTGCCTGGGGGAAAACTAGAGAGCATACCACTTCTGCAGGGGGCGCAGATAAGGAAAGTGAAAGTGATACATCATCGTGGGGTAAAACTCTACCTAAAGAACCTCCCGCATCTACTTGGGGGATAGCCAAGGCAGCAGAAGATACCACTCCAGCATGGGGGACAAATACTGCCAGAGAAGATGCCACATCTGCCTGGGGGAAAACTGGAGAACCTACCACTTCTGCCTGGGGGGCAAGTGAAAAAGATACACCATCTTGGGGGAAAACTCCAGCTAAAGAAACTTTGACATCCACTTGGGGGATAGATAAGGCAGCAGAAGATACCGCTCCTGCATGGGGGACAAGTACTGCCAGAGAAGATGCCACACCTGCCTGGGGGAAATCTAGAGAAAAGACTACTTCCGCCTGGGGAACAGATAAGGAAAGTGATAATGATACATCATCTTGGGGGAAAAATCCAGCTAGAAAACCTACCACCTCTACTTGGGGCATAGATAAGGTAGCAGAAGATACCACTCCTGCATGGGGAACAAGTACTGCCACAGAAGATGCCACACCTGCCTGGGGGAAATCTAGAGAAAAAACTGCTTCTGCCTGGGGAACAAATAAGGAAAGTGAAAATGACACATCATCTTGGGGGAAAACTCCAGCCAGAAAACCTACCAACTCTACTTGGGGGACAGAGAAGGCAGCAGAAGATACCACTCCTACGTCAGGGACAAATACTGCCAGAGAAGATGCCACATCTACCTGGGGGAAAACTAGAGAACATACCACTTCTACCTGGGGGACAGATAAGGCAAGTGAAAATGTTTGGAGTGGTAGAGGGGCAGAGAAAGTCGCACCAATAGACCTCCCGCCAACAACAAAAGACGTAGATGAAAAGGACAATGGATGGGGTGCTGTTTCTTCTGGGGAAACAGAAACAGGGAATGAAAATGCTTGGGAACAGAATAAGTCTTCCGAAATTTCGACTGGTTCTTGGGGGCAGAAAAATCCTCCCGAGAAAGAAACTCAACCTGCAAGCTTGTCTGGGTGGGATTCACCTAGTGGAGACGTCGTTTTGAAGGTTCAAAGAGTTGGGTTTCTAGTCCCGGGGAGTGGAAGAATAAGAACCGTCCTGCTAAACCACCTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1762

Amino Acids

194.03

Weight (kDa)

5.81

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 46 - 281 4.7e-12 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 324 - 478 6.7e-33 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 481 - 626 1.6e-11 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 754 - 1181 1.8e-09 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 4522, 4711
AatII GACGTC 1 cut(s) 5205
Acc36I ACCTGC 4 cut(s) 1597, 4522, 4711, 5173
Acc65I GGTACC 1 cut(s) 1092
AccB1I GGYRCC 1 cut(s) 1092
AccBSI CCGCTC 2 cut(s) 984, 4472
AccI GTMKAC 1 cut(s) 2877
AccII CGCG 2 cut(s) 704, 3549
AclI AACGTT 1 cut(s) 2457
AclWI GGATC 7 cut(s) 701, 714, 2261, 2470, 3176, 3693, 3706
AcoI YGGCCR 3 cut(s) 1482, 1557, 2367
AcuI CTGAAG 2 cut(s) 1917, 3090
AcyI GRCGYC 1 cut(s) 5202
AdeI CACNNNGTG 2 cut(s) 1349, 5195
AflIII ACRYGT 3 cut(s) 1884, 3494, 3679
AhlI ACTAGT 1 cut(s) 2236
AjuI GAANNNNNNNTTGG 8 cut(s) 198, 230, 2557, 2589, 2853, 2885, 3955, 3987
AloI GAACNNNNNNTCC 2 cut(s) 647, 679
Alw21I GWGCWC 3 cut(s) 2634, 3791, 3823
Alw26I GTCTC 4 cut(s) 398, 864, 3074, 5193
AlwI GGATC 7 cut(s) 701, 714, 2261, 2470, 3176, 3693, 3706
AlwNI CAGNNNCTG 1 cut(s) 1555
Ama87I CYCGRG 2 cut(s) 5147, 5239
ArsI GACNNNNNNTTYG 2 cut(s) 1223, 1255
Asp700I GAANNNNTTC 3 cut(s) 964, 2480, 2945
Asp718I GGTACC 1 cut(s) 1092
AspA2I CCTAGG 1 cut(s) 2116
AspLEI GCGC 3 cut(s) 3420, 3551, 4190
AspS9I GGNCC 6 cut(s) 1231, 1251, 1340, 2319, 2346, 2469
AsuC2I CCSGG 2 cut(s) 5240, 5241
AvaI CYCGRG 2 cut(s) 5147, 5239
AvaII GGWCC 3 cut(s) 1231, 2319, 2346
AvrII CCTAGG 1 cut(s) 2116
BaeGI GKGCMC 1 cut(s) 1182
BaeI ACNNNNGTAYC 2 cut(s) 2739, 2772
BalI TGGCCA 3 cut(s) 1484, 1559, 2369
BamHI GGATCC 2 cut(s) 706, 3698
BanI GGYRCC 1 cut(s) 1092
BanII GRGCYC 1 cut(s) 2634
BauI CACGAG 3 cut(s) 867, 2301, 3726
BbsI GAAGAC 2 cut(s) 2162, 5097
Bbv12I GWGCWC 3 cut(s) 2634, 3791, 3823
BceAI ACGGC 1 cut(s) 2628
BcgI CGANNNNNNTGC 4 cut(s) 703, 737, 2877, 2911
BciVI GTATCC 3 cut(s) 617, 1921, 2226
BclI TGATCA 2 cut(s) 517, 3403
BcnI CCSGG 2 cut(s) 5240, 5241
BcoDI GTCTC 4 cut(s) 398, 864, 3074, 5193
BcuI ACTAGT 1 cut(s) 2236
BfmI CTRYAG 5 cut(s) 1132, 1820, 2223, 2751, 4179
BfoI RGCGCY 1 cut(s) 3421
BfuAI ACCTGC 4 cut(s) 1597, 4522, 4711, 5173
BfuI GTATCC 3 cut(s) 617, 1921, 2226
BglI GCCNNNNNGGC 1 cut(s) 4376
BglII AGATCT 1 cut(s) 3029
BlnI CCTAGG 1 cut(s) 2116
BlpI GCTNAGC 4 cut(s) 281, 346, 1448, 1548
BmcAI AGTACT 3 cut(s) 1688, 4492, 4681
Bme18I GGWCC 3 cut(s) 1231, 2319, 2346
BmeT110I CYCGRG 2 cut(s) 5147, 5239
BmgT120I GGNCC 6 cut(s) 1231, 1251, 1340, 2319, 2346, 2469
BmiI GGNNCC 9 cut(s) 708, 1094, 1171, 1232, 1253, 2114, 2347, 2471, 3700
BmrI ACTGGG 2 cut(s) 864, 3833
BmuI ACTGGG 2 cut(s) 864, 3833
BoxI GACNNNNGTC 1 cut(s) 3897
BpiI GAAGAC 2 cut(s) 2162, 5097
BplI GAGNNNNNCTC 5 cut(s) 28, 2204, 2236, 2984, 3016
BpmI CTGGAG 7 cut(s) 572, 3275, 4029, 4278, 4371, 4398, 4776
Bpu1102I GCTNAGC 4 cut(s) 281, 346, 1448, 1548
BpuEI CTTGAG 5 cut(s) 176, 558, 600, 2308, 2680
BpuMI CCSGG 2 cut(s) 5240, 5241
BsaBI GATNNNNATC 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 5202
BsaI GGTCTC 1 cut(s) 3074
BsaXI ACNNNNNCTCC 4 cut(s) 2442, 2472, 5190, 5220
Bse1I ACTGG 9 cut(s) 233, 859, 1560, 2018, 2988, 3700, 3828, 4354, 5125
Bse3DI GCAATG 4 cut(s) 1652, 2292, 2529, 2995
Bse8I GATNNNNATC 1 cut(s) 834
BseJI GATNNNNATC 1 cut(s) 834
BseMI GCAATG 4 cut(s) 1652, 2292, 2529, 2995
BseMII CTCAG 7 cut(s) 272, 534, 663, 999, 1562, 1782, 1943
BseNI ACTGG 9 cut(s) 233, 859, 1560, 2018, 2988, 3700, 3828, 4354, 5125
BseRI GAGGAG 5 cut(s) 20, 1079, 2291, 2861, 3945
BseSI GKGCMC 1 cut(s) 1182
BsgI GTGCAG 3 cut(s) 369, 2721, 3039
Bsh1236I CGCG 2 cut(s) 704, 3549
BshNI GGYRCC 1 cut(s) 1092
BsiHKAI GWGCWC 3 cut(s) 2634, 3791, 3823
BsiHKCI CYCGRG 2 cut(s) 5147, 5239
BsiSI CCGG 1 cut(s) 5240
BsmAI GTCTC 4 cut(s) 398, 864, 3074, 5193
BsmBI CGTCTC 1 cut(s) 5193
BsmI GAATGC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 3074
BsoBI CYCGRG 2 cut(s) 5147, 5239
Bsp1286I GDGCHC 4 cut(s) 1182, 2634, 3791, 3823
Bsp1407I TGTACA 1 cut(s) 2098
Bsp1720I GCTNAGC 4 cut(s) 281, 346, 1448, 1548
Bsp19I CCATGG 1 cut(s) 2257
BspCNI CTCAG 7 cut(s) 273, 535, 664, 998, 1561, 1781, 1944
BspFNI CGCG 2 cut(s) 704, 3549
BspHI TCATGA 1 cut(s) 2763
BspLI GGNNCC 9 cut(s) 708, 1094, 1171, 1232, 1253, 2114, 2347, 2471, 3700
BspMAI CTGCAG 3 cut(s) 2227, 2755, 4183
BspMI ACCTGC 4 cut(s) 1597, 4522, 4711, 5173
BspPI GGATC 7 cut(s) 701, 714, 2261, 2470, 3176, 3693, 3706
BspT107I GGYRCC 1 cut(s) 1092
BspTNI GGTCTC 1 cut(s) 3074
BsrBI CCGCTC 2 cut(s) 984, 4472
BsrDI GCAATG 4 cut(s) 1652, 2292, 2529, 2995
BsrGI TGTACA 1 cut(s) 2098
BsrI ACTGG 9 cut(s) 233, 859, 1560, 2018, 2988, 3700, 3828, 4354, 5125
BssNI GRCGYC 1 cut(s) 5202
BssSI CACGAG 3 cut(s) 867, 2301, 3726
BssT1I CCWWGG 4 cut(s) 142, 2116, 2257, 4272
Bst2BI CACGAG 3 cut(s) 867, 2301, 3726
Bst4CI ACNGT 9 cut(s) 659, 951, 1497, 1676, 2407, 2669, 2908, 2977, 5263
Bst6I CTCTTC 4 cut(s) 919, 982, 2921, 3066
BstACI GRCGYC 1 cut(s) 5202
BstAPI GCANNNNNTGC 2 cut(s) 1610, 1658
BstAUI TGTACA 1 cut(s) 2098
BstC8I GCNNGC 6 cut(s) 149, 343, 1615, 1659, 2818, 5170
BstDSI CCRYGG 2 cut(s) 702, 2257
BstEII GGTNACC 1 cut(s) 3143
BstENI CCTNNNNNAGG 1 cut(s) 4857
BstFNI CGCG 2 cut(s) 704, 3549
BstH2I RGCGCY 1 cut(s) 3421
BstHHI GCGC 3 cut(s) 3420, 3551, 4190
BstMAI GTCTC 4 cut(s) 398, 864, 3074, 5193
BstNSI RCATGY 4 cut(s) 1888, 2709, 3498, 3683
BstPAI GACNNNNGTC 1 cut(s) 3897
BstPI GGTNACC 1 cut(s) 3143
BstSFI CTRYAG 5 cut(s) 1132, 1820, 2223, 2751, 4179
BstSLI GKGCMC 1 cut(s) 1182
BstUI CGCG 2 cut(s) 704, 3549
BstV2I GAAGAC 2 cut(s) 2162, 5097
BstX2I RGATCY 5 cut(s) 706, 2266, 2462, 3029, 3698
BstYI RGATCY 5 cut(s) 706, 2266, 2462, 3029, 3698
BsuI GTATCC 3 cut(s) 617, 1921, 2226
BtgI CCRYGG 2 cut(s) 702, 2257
BtsI GCAGTG 2 cut(s) 1752, 2517
BtsIMutI CAGTG 9 cut(s) 947, 993, 1437, 1485, 1493, 1752, 2517, 2995, 3821
BveI ACCTGC 4 cut(s) 1597, 4522, 4711, 5173
Cac8I GCNNGC 6 cut(s) 149, 343, 1615, 1659, 2818, 5170
CaiI CAGNNNCTG 1 cut(s) 1555
CciI TCATGA 1 cut(s) 2763
CfoI GCGC 3 cut(s) 3420, 3551, 4190
Cfr13I GGNCC 6 cut(s) 1231, 1251, 1340, 2319, 2346, 2469
Cfr42I CCGCGG 1 cut(s) 705
Cfr9I CCCGGG 1 cut(s) 5239
CseI GACGC 1 cut(s) 623
CsiI ACCWGGT 2 cut(s) 469, 5277
DraI TTTAAA 2 cut(s) 1519, 2356
DraIII CACNNNGTG 2 cut(s) 1349, 5195
EaeI YGGCCR 3 cut(s) 1482, 1557, 2367
Eam1104I CTCTTC 4 cut(s) 919, 982, 2921, 3066
EarI CTCTTC 4 cut(s) 919, 982, 2921, 3066
EciI GGCGGA 1 cut(s) 4537
Ecl136II GAGCTC 1 cut(s) 2632
Eco130I CCWWGG 4 cut(s) 142, 2116, 2257, 4272
Eco147I AGGCCT 4 cut(s) 11, 1195, 2121, 2820
Eco24I GRGCYC 1 cut(s) 2634
Eco31I GGTCTC 1 cut(s) 3074
Eco32I GATATC 1 cut(s) 3712
Eco47I GGWCC 3 cut(s) 1231, 2319, 2346
Eco53kI GAGCTC 1 cut(s) 2632
Eco57I CTGAAG 2 cut(s) 1917, 3090
Eco88I CYCGRG 2 cut(s) 5147, 5239
Eco91I GGTNACC 1 cut(s) 3143
EcoICRI GAGCTC 1 cut(s) 2632
EcoNI CCTNNNNNAGG 1 cut(s) 4857
EcoO109I RGGNCCY 4 cut(s) 1231, 1251, 2346, 2469
EcoO65I GGTNACC 1 cut(s) 3143
EcoRI GAATTC 1 cut(s) 2759
EcoRV GATATC 1 cut(s) 3712
EcoT14I CCWWGG 4 cut(s) 142, 2116, 2257, 4272
EcoT22I ATGCAT 2 cut(s) 1006, 3289
EcoT38I GRGCYC 1 cut(s) 2634
ErhI CCWWGG 4 cut(s) 142, 2116, 2257, 4272
Esp3I CGTCTC 1 cut(s) 5193
FalI AAGNNNNNCTT 8 cut(s) 915, 947, 2724, 2756, 3453, 3485, 3570, 3602
FauI CCCGC 4 cut(s) 989, 4114, 4258, 5010
FauNDI CATATG 2 cut(s) 3355, 3780
FbaI TGATCA 2 cut(s) 517, 3403
FblI GTMKAC 1 cut(s) 2877
FriOI GRGCYC 1 cut(s) 2634
GlaI GCGC 3 cut(s) 3419, 3550, 4189
GsuI CTGGAG 7 cut(s) 572, 3275, 4029, 4278, 4371, 4398, 4776
HaeII RGCGCY 1 cut(s) 3421
HapII CCGG 1 cut(s) 5240
HgaI GACGC 1 cut(s) 623
HhaI GCGC 3 cut(s) 3420, 3551, 4190
Hin1I GRCGYC 1 cut(s) 5202
Hin6I GCGC 3 cut(s) 3418, 3549, 4188
HinP1I GCGC 3 cut(s) 3418, 3549, 4188
HincII GTYRAC 3 cut(s) 125, 1959, 2455
HindII GTYRAC 3 cut(s) 125, 1959, 2455
HindIII AAGCTT 2 cut(s) 924, 5170
HpaII CCGG 1 cut(s) 5240
Hpy166II GTNNAC 8 cut(s) 125, 1246, 1959, 2287, 2455, 2495, 2878, 3677
Hpy8I GTNNAC 8 cut(s) 125, 1246, 1959, 2287, 2455, 2495, 2878, 3677
Hpy99I CGWCG 2 cut(s) 3163, 5207
HpyCH4III ACNGT 9 cut(s) 659, 951, 1497, 1676, 2407, 2669, 2908, 2977, 5263
HpyCH4IV ACGT 8 cut(s) 1758, 2159, 2457, 2698, 3343, 4856, 5019, 5202
HpySE526I ACGT 8 cut(s) 1758, 2159, 2457, 2698, 3343, 4856, 5019, 5202
Hsp92I GRCGYC 1 cut(s) 5202
HspAI GCGC 3 cut(s) 3418, 3549, 4188
KpnI GGTACC 1 cut(s) 1096
Ksp22I TGATCA 2 cut(s) 517, 3403
KspI CCGCGG 1 cut(s) 705
LmnI GCTCC 6 cut(s) 901, 1420, 2584, 2848, 3818, 4477
MabI ACCWGGT 2 cut(s) 469, 5277
MaeII ACGT 8 cut(s) 1758, 2159, 2457, 2698, 3343, 4856, 5019, 5202
MaeIII GTNAC 4 cut(s) 228, 2663, 3143, 3433
MbiI CCGCTC 2 cut(s) 984, 4472
MfeI CAATTG 2 cut(s) 437, 822
MflI RGATCY 5 cut(s) 706, 2266, 2462, 3029, 3698
MhlI GDGCHC 4 cut(s) 1182, 2634, 3791, 3823
MlsI TGGCCA 3 cut(s) 1484, 1559, 2369
MluNI TGGCCA 3 cut(s) 1484, 1559, 2369
MlyI GAGTC 6 cut(s) 2002, 2048, 2060, 2460, 3029, 3887
MmeI TCCRAC 5 cut(s) 25, 156, 353, 2440, 3057
Mox20I TGGCCA 3 cut(s) 1484, 1559, 2369
Mph1103I ATGCAT 2 cut(s) 1006, 3289
MroXI GAANNNNTTC 3 cut(s) 964, 2480, 2945
MscI TGGCCA 3 cut(s) 1484, 1559, 2369
MslI CAYNNNNRTG 4 cut(s) 2522, 3015, 3667, 4219
Msp20I TGGCCA 3 cut(s) 1484, 1559, 2369
MspA1I CMGCKG 4 cut(s) 704, 1444, 3431, 3620
MspI CCGG 1 cut(s) 5240
MunI CAATTG 2 cut(s) 437, 822
Mva1269I GAATGC 1 cut(s) 343
MvnI CGCG 2 cut(s) 704, 3549
NciI CCSGG 2 cut(s) 5240, 5241
NcoI CCATGG 1 cut(s) 2257
NdeI CATATG 2 cut(s) 3355, 3780
NlaIV GGNNCC 9 cut(s) 708, 1094, 1171, 1232, 1253, 2114, 2347, 2471, 3700
NmeAIII GCCGAG 1 cut(s) 2717
NmuCI GTSAC 1 cut(s) 3143
NsiI ATGCAT 2 cut(s) 1006, 3289
NspI RCATGY 4 cut(s) 1888, 2709, 3498, 3683
PagI TCATGA 1 cut(s) 2763
PaqCI CACCTGC 2 cut(s) 4522, 4711
PceI AGGCCT 4 cut(s) 11, 1195, 2121, 2820
PciI ACATGT 3 cut(s) 1884, 3494, 3679
PctI GAATGC 1 cut(s) 343
PdmI GAANNNNTTC 3 cut(s) 964, 2480, 2945
PleI GAGTC 6 cut(s) 2001, 2047, 2060, 2459, 3029, 3887
PpsI GAGTC 6 cut(s) 2001, 2047, 2060, 2459, 3029, 3887
PpuMI RGGWCCY 2 cut(s) 1231, 2346
PscI ACATGT 3 cut(s) 1884, 3494, 3679
PshAI GACNNNNGTC 1 cut(s) 3897
Psp124BI GAGCTC 1 cut(s) 2634
Psp1406I AACGTT 1 cut(s) 2457
Psp5II RGGWCCY 2 cut(s) 1231, 2346
PspEI GGTNACC 1 cut(s) 3143
PspN4I GGNNCC 9 cut(s) 708, 1094, 1171, 1232, 1253, 2114, 2347, 2471, 3700
PspPI GGNCC 6 cut(s) 1231, 1251, 1340, 2319, 2346, 2469
PspPPI RGGWCCY 2 cut(s) 1231, 2346
PstI CTGCAG 3 cut(s) 2227, 2755, 4183
PstNI CAGNNNCTG 1 cut(s) 1555
PsuI RGATCY 5 cut(s) 706, 2266, 2462, 3029, 3698
PvuII CAGCTG 3 cut(s) 1444, 3431, 3620
RseI CAYNNNNRTG 4 cut(s) 2522, 3015, 3667, 4219
SacI GAGCTC 1 cut(s) 2634
SacII CCGCGG 1 cut(s) 705
Sau96I GGNCC 6 cut(s) 1231, 1251, 1340, 2319, 2346, 2469
ScaI AGTACT 3 cut(s) 1688, 4492, 4681
SchI GAGTC 6 cut(s) 2002, 2048, 2060, 2460, 3029, 3887
SduI GDGCHC 4 cut(s) 1182, 2634, 3791, 3823
SexAI ACCWGGT 2 cut(s) 469, 5277
SfcI CTRYAG 5 cut(s) 1132, 1820, 2223, 2751, 4179
Sfr303I CCGCGG 1 cut(s) 705
SgrBI CCGCGG 1 cut(s) 705
SinI GGWCC 3 cut(s) 1231, 2319, 2346
SmaI CCCGGG 1 cut(s) 5241
SmiMI CAYNNNNRTG 4 cut(s) 2522, 3015, 3667, 4219
SmlI CTYRAG 5 cut(s) 155, 573, 615, 2323, 2659
SmoI CTYRAG 5 cut(s) 155, 573, 615, 2323, 2659
SpeI ACTAGT 1 cut(s) 2236
SseBI AGGCCT 4 cut(s) 11, 1195, 2121, 2820
SspI AATATT 4 cut(s) 25, 243, 1921, 3559
SstI GAGCTC 1 cut(s) 2634
StuI AGGCCT 4 cut(s) 11, 1195, 2121, 2820
StyI CCWWGG 4 cut(s) 142, 2116, 2257, 4272
TaaI ACNGT 9 cut(s) 659, 951, 1497, 1676, 2407, 2669, 2908, 2977, 5263
TaiI ACGT 8 cut(s) 1761, 2162, 2460, 2701, 3346, 4859, 5022, 5205
TaqI TCGA 9 cut(s) 14, 713, 1996, 2064, 2153, 2887, 3151, 3303, 5117
TaqII GACCGA 2 cut(s) 2307, 2351
TatI WGTACW 5 cut(s) 1686, 2098, 2655, 4490, 4679
TscAI CASTG 9 cut(s) 954, 993, 1437, 1492, 1500, 1752, 2524, 2995, 3828
TseFI GTSAC 1 cut(s) 3143
Tsp45I GTSAC 1 cut(s) 3143
TspGWI ACGGA 2 cut(s) 541, 655
TspMI CCCGGG 1 cut(s) 5239
TspRI CASTG 9 cut(s) 954, 993, 1437, 1492, 1500, 1752, 2524, 2995, 3828
VpaK11BI GGWCC 3 cut(s) 1231, 2319, 2346
XagI CCTNNNNNAGG 1 cut(s) 4857
XbaI TCTAGA 3 cut(s) 3901, 4529, 4718
XceI RCATGY 4 cut(s) 1888, 2709, 3498, 3683
XcmI CCANNNNNNNNNTGG 2 cut(s) 474, 2444
XmaI CCCGGG 1 cut(s) 5239
XmaJI CCTAGG 1 cut(s) 2116
XmiI GTMKAC 1 cut(s) 2877
XmnI GAANNNNTTC 3 cut(s) 964, 2480, 2945
ZraI GACGTC 1 cut(s) 5203
ZrmI AGTACT 3 cut(s) 1688, 4492, 4681
Zsp2I ATGCAT 2 cut(s) 1006, 3289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.