FvH4_7g03040

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006658
Physical Location & Seq
Forward (+)
3401866 .. 3418492
16627 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03040.t2

Sequence Viewer

Length: 6039 bp
ATGGAGGAAGCTTCTTGCTCACCGGTTTTAGACGGGGAGCTGATTGGGATCAGATTCGGATTAGCAACCCATCAAGAAATTTGTACAGCATCGATGATGGGATGCTCTATAAGCCACGCTAGTCAACTATCTAACCCATTCCTTGGCCTTCCTCTTGAGTTTGGAAAGTGTGAATCTTGTGGTACATCCGAACCTGGAAAGTGCGAAGGACACTTTGGATACATTGACTTACCAGTACCCATATATCATCCTAACCATGTCAGTGAGCTGAAGAAGTTGTTGAGTCTCTTGTGCTTGAAATGCTTAAAAATGAAAAAGAACAAGGTCCCAACCAAGAGTGCCGGTTTGGCCGAAAGATTGTTATCAGTATGTTGTGAGGATGCTGCACAAGTTTCTATCACAGAGTTCAAGCCAAGAGATGGGGTTTGTTCCTTGCAATTGAAACTACCTTCTAACAAAAAACCACCGCCTGGTTTTTGGAATTTCTTGGAGAGATATGGCTTTCGCTATGGTGATGGTGTAAAACGCATTTTGCTTCCTAGAGAGGTGATGCAGATTCTCAGAAGAATCCCTGAAGAGACAAAGAAAAAACTGGCGACTAGAGGCTATAATCCTCAAGATGGATACATCTTGAATCATATACCTGTCCCTCCTAATTGCTTGTCAGTGCCTGAAATATCTGATGGTGTTACCATTATCTCTGCGGATCCTTCAATATCAATGCTCAAAAAGGTTCTAAGAAGAGTTGAAGAGATCAGTAGTTCAAGGTCTGGTGCAGCAAATTTTGAATCTGAAATTGACGAGGTTGATTTGTTGCAAGAATCAGTTGATCAGTATCTTCAAGTTAGGGGTACTGGAAAGGCATCACGTGAAAGTGATGCAAGATTCGGTGGGACCAAGGAGCTCAGTGAATCTTCGACAAAAGCATGGCTCGAAAAAATGAGGACATTGTTTATCAGAAAGGGTTCAGGATTCTCTTCCCGCACAGTGATTACGGGGGATGCATATAGAAGGGTGAATGAAGTTGGTATCCCATATGATATTGCCCAAAGAATTACATTTGAGGAGAAGGTCAATGCACATAATATTAGGCGTCTTCAAGAGCTGGTAGATAGCAAGTTATGCCTAACCTACAGCGATGGTTCATCAACTTACTCTTTAAGAGAAGGTTCAAAGGGGCACACATTTTTGAAGCCTGGTCAAGTGGTGCATAGGAGGATAATGGATGGGGATCTTGTTTTTGTAAATAGGCCTCCAACTACGCATAAACATTCTCTGCAAGCACTGCAAGTTTATGTGCATGAGGACAAGGTGGTAAAGATAAATCCTCTGATTTGTGGGCCACTAAGTGCTGACTTTGATGGAGATTGCATTCATCTGTTCTATCCCCAATCACTTGCGGCAAAGGCAGAAGTTATGGAGCTTTTTTCAGTGGAGAAGCAGCTGCTTAGCTCTCACAGTGGCAAGCCTAATTTGCAACTGGCCACTGATTCGCTTTTGTCGTTAAAACTGATGTTTAAGAAATACTTTTTTAACAAAGCAGCAATGCAGCAGTTGGCCATGTTTGTTTCGTCTTCATTGCCACAACCAGCTTTATTGAAAGCTAATTCTACAGTTCCATGTTGGACTGCTTTGCAGATATTGCAGACTGCATTGCCTGCTCAGTTTCAGTCCTCTGGTGACAGACACTTGGTCAAGGATAGTGAGGTGCTCTTACTTGATTGTAGTACTAGTCTTGTACCATCTCTGATCAATGACATTGGTACCTCTATTTTCTTTGAGAAAAGTGCTGAAGATGTTCTCAGTTTCTTTAATTCTATGCAGCCATTGCTGATGGAGAATTTATTTTCAGAAGGGTTCAGTGTGGGTTTGGAGGATTTTGCTTTGCCTAGGGCATCTATACAAGACATCCAAAAGGGCCTTAAGGAAATTTCTCCTTTGTTGTTCCATATGAGGACAGTATATAATGAGCTTGTTGAGATGCAATTGGAGAATCACATCCGCAAAGTAAAAGAACCGGTCTCGAATTTTATATTAAATTCATCGGCATTGGGTGATTTAATTGACTCTAAAAGTGATTCTGCAATGAACAAAGTAGTTCAACAAGTTGGATTTCTAGGCCGGCAACTGTATGACCAGGGGAAACTCTATTCAAAAACATTGGTTGAGGATGTGAGCTCACTGTATCAGAACAAGTATCCATCTGACATTGTGGATTATCCCTCAGCAGAATTCGGATTGGTTCATAGGGGTTTTGTTCATGGGTTGGATCCAGTTGAGGGAATGATTCATTCCATTGCTACAAGAGAAGTAATTGTTCGATCCTCGAGAGGTTTGTCTGAACCGGGGACTCTATTCAAGAATCTTATGGCTACACTGCGAGACGTTGTCATTTGCTATGATGGCACAGTGAGGAATGTCTGTAGTAATTCCATAATTCAGTTCGAATATGGCGTGAAGAGTGGATCTGGTCCGGAGAATTTGTTCCCTGCTGGTGAACCTGTAGGTGTGTTAGCTGCAACAGCAATGTCAAATCCAGCATACAAGGCAGTTCTTGATTCTACGCCAAGCAGCAACTCTTCATGGGATTTGATGAAGGAAATACTACTCTGCAAAGTCAATTTCAAGAATGAGCTCATTGATCGTCGTGTAGTCCTGTATCTGAATGATTGTGGTTGTGGAAGAAAATACTGCAGAGAACATGCGGCATATTTGGTCAAGAATCGTTTGAAAAAAGTCAGCCTTAAAGATACTGCTGTCGAGTTTATGATTGAATATCAAAAAGAGCGAGCTGCAGGAAGCATGGAAATCGACTCTGGCCTTGTTGGTCATGTTCATTTGAATGAGATGCTGTTACGGGAGCTGAATCTAGGAATGTCTGAGATCCTTCAAAAATGCGAAGAAACTATCAATTCATTTCGGAGAAAGAAGGTCGGGAAGAAGATGAACATTGGCGAAATTTTTAAAAGGACAATTTTGTCGTACAGTGAATGTTGCTCTTTTCATCAGTCCTCTGCTGACAATAGATCTGGATCCCCTTGCTTGATGTTCTTCTACCAAGATTTTAATAATTCAGAGTTAGAGGCCATTTCACAAATGTTGGCCGACTTTATTTGCCCAGTTCTTCTAAAAACTGTAATTAAAGGTGATCCTCGAATTTCGTCGGCCAATATAATTTGGATCAATTCAGAATCAACAACTTGGATTAGAAGCCCAAACAAATCTCTGAAGGGTGAATTGGCTTTGGATGTTGTTCTGGAGAAGTCGGTTGTTAAACAAAGTGGTGATGCGTGGAGAATAGCCCTTGATTGTTGCCTTCCAGTGCTTCATTTGATTGACACCAGGCGTTCCATTCCATATGCCATCAAACAAGTGCAGGAATTGTTGGGGGTTTCTTGTGCTTTTGATCAAGCAGTTCAGCGCCTTGCGAAAGCTGTTGCAATGGTAGCAAAAGGTGTTCTCAAAGAGCATCTTATTCTGTTGGCAAATAGTATGACATGCGCTGGAAATTTCGTTGGATTCAATCCTGGTGGTTATAAAGCACTATCTCGCGCATTGAATATACAAGTACCCTTCACAGAAGCAACATTGTTTACACCAAAAAAGTGTTTTGAGAGAGCAGCTGAAAAGTGTCATATGGATTCATTGTCGAGCATAGTTGCATCATGTTCGTGGGGTAAACATGTAGCAGTTGGTACTGGCTCCAGGTTCGAAATCCTCTGGGACACAAAAGAGGGAGGCTTGAACGAAGTGGGTGGCGTAGATGTTTTCAACTTTCTACATATGGTGAACGCTGCAAATGGAGAAGAATTAACTACTGCAGCTTTGGGAACAGAAATTGATGATCTTGTGCCGGATTATGAAAATGGAGATGTGTCCTTGTCACCAGAGCATAATTCTAGTTCTGATAAGCCAGTGTTTGAAGATATTGTCGAGTTCCCTGATAATTTTGAAAATGTTCCTGGAAAATCGAGCTGGGATTCAATCTACACCGCTTCTACTGGTGGGAAAGACTGGGGGGCAGTTAAAATTGGAACACAAGATGGCATTTCAGCTGAAACCCAAGCGGATAGCACATCTGCGTGGGGGATTAAGACTCCTAGAGAAGATGATACATCTCCCTGGGGAACAGCCAAGACTGCTACAGAAGATGCCCCATCTGCGTGGGGTACCAAGACTGTTAAAGAAGATGCCCCATCTGCATGGGGGATTAAGACAGCGAGAGAAGACGCTCGATCTCCCTGGGGAACAGCTAAGACTGCTACAGAAGATGCCTCATCTGCATGGGGGACCAAGACTGTTAAAGAAGATGCCCCATCTGCATGGGGGATTAAGACGGCGAGAGAAGACGCTCCATCTCCATGGGGAACAGCCAAAACTGCTACAGGAGATGCTCTGTCTGCTTGGGGGACCAAGACTCTTAAAGAAGATGCCCCATCTGCATGGGGGACTAAGACTGTGAGAGAAGATGCTCCATCTCCATGGGGAACAGCCAAGAGAGCTACAGAAGATGCTCCGGCTGCATGGGGCGCCAAGTCAGCTACAGAAGATGCTCCGCCTCCATGGGGAACAGCCAAGACAGCTGCAGAAGATGCTCCGTCTGCATGGGGCGTCAAGGCTGTTAAAAAAGATGCCTCATCTCCATGGGAGACAAAGACTGCAACAGAAGATGCCACTACTGCATGGGGGAGAAACAATGAAAAGAAACATGTCCTCACTGCAGAAGTCCTTGAGGATTCCAAGGCTAGAGAAGATGCCACATCTGGCTGGGGGACAGAAAATGCAGAAAATGCACAATCTACATGGAGAACCAGTACAGAAAGTGAGAATGGTTGGTCTGGTAGGGGGGGGAGTAAAGTTGAATCAACAGATGTTCAGTCCCAGAAAGCAGTAGAAAACCCCAAGGGTTGGAATGACTTCTCTGCTGGGGTAAGAAAACCCCAGACGGAGAATGCTGGGTCTGGATGGGGCATGAAAGGTTCTGAGAAGAAAGGTGATATTGAGTTGGAACAAGACGAGTCTACCCGTCATTCTTGGAAGCAGAAGTCTGCTGATGCTTCTTCTCAGGGTGCTTGGGAACGGCAGAAGTCTCCTGATACTTCCAAAGGCACATGGGAGCAGCCAAAGTCTGCTGAGATGTCTCACGGCGCTTGGGGACAGCAAAAGTCTCCCGATGTCTCTCAAGGTGTATGGGGGCTGGAAAAGCCTGCTAGTACCAATTCTCAAGGCTCATGGGGACAGCAAAAGTCTCCTGAGATACCTCAGGGTAACTGGGGACAGCAAACGTCTCCTGATATTTCTCAAGGCACATGGGGGCAGCAAAAGTCTCCTGAGATGTCTCAAGGCTCTTGGGGACAGCAGAAACCTTCTGACACTTCTCAACCTGCAACTGTGAATCAGTGGGATTCTCAAAGTGAAGCAGCTGTTGAAAGACATCAACAATGGGGTCATAATGGAGATTCAAACAAGAGAAAACGTTTTGAAGGTGGCAGGAGTTGGGGTCCAAATGCTGGGGAGTGGAAGGGTAAGAATAGTCGCCCTGCTAAATCACCTGGAATGGTGAATGATGATTCTAGTGTCGCTGCAATATATACCGCAACACGGCAAAGATTGGACATTTTCACATCTGAGGAGCAAGATGTTCTTTCCCATATTGAGCCGACTATGCTCTCTATTCGAAGAATAATGCATCAATCTGGTTACAATGATGGAGATCAATTATCAGCTGAGGATCACTCATTCATCCTTGATAAAGTGTTCAATTTCCATCCGGAGAAAGAGGCCAAGATGGGTTCTGGAATCGACTATTTTACAGTTGATAGACATGGCAGCTTTCAAGAAAGCAGATGCCTCTTTGTCATATCAACTGATGGTCGCAAGGAGGACTTTTCCTACAGGAAATGCCTGCAGAACATGATCAAGGAAAAGTATCCTGAGCTAGCTGAAGCCTTCAATGACAAATATTTCAGCAGACCTCGCCCCCGGAATCCCATGTTCGGTCAGACCCCTCCAGAGAGAACTGGGAACGAGACACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

2013

Amino Acids

221.76

Weight (kDa)

6.05

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 48 - 286 1.6e-13 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 324 - 478 9.6e-34 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 481 - 626 2e-09 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5 PF04998 755 - 1181 1e-09 RNA polymerase Rpb1, domain 5
DUF3223 PF11523 1854 - 1929 3.9e-28 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3537
AasI GACNNNNNNGTC 1 cut(s) 2977
Acc36I ACCTGC 1 cut(s) 5392
Acc65I GGTACC 2 cut(s) 1763, 4169
AccB1I GGYRCC 3 cut(s) 1763, 4169, 4529
AccB7I CCANNNNNTGG 5 cut(s) 143, 419, 470, 4908, 5510
AccI GTMKAC 1 cut(s) 5021
AccII CGCG 1 cut(s) 3552
AccIII TCCGGA 2 cut(s) 2473, 5770
AclI AACGTT 1 cut(s) 5476
AcoI YGGCCR 5 cut(s) 348, 1482, 1557, 3102, 3165
AcuI CTGAAG 5 cut(s) 290, 594, 1812, 3248, 5964
AcvI CACGTG 1 cut(s) 869
AcyI GRCGYC 3 cut(s) 1093, 4530, 4611
AdeI CACNNNGTG 1 cut(s) 1349
AflII CTTAAG 1 cut(s) 1922
AflIII ACRYGT 2 cut(s) 3682, 4708
AgeI ACCGGT 2 cut(s) 22, 2017
AhdI GACNNNNNGTC 1 cut(s) 1691
AhlI ACTAGT 1 cut(s) 1730
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 3221, 3253
AloI GAACNNNNNNTCC 2 cut(s) 2468, 2500
Alw21I GWGCWC 4 cut(s) 906, 1713, 2180, 2637
AlwNI CAGNNNCTG 1 cut(s) 671
Ama87I CYCGRG 1 cut(s) 2326
Aor13HI TCCGGA 2 cut(s) 2473, 5770
AsiGI ACCGGT 2 cut(s) 22, 2017
Asp700I GAANNNNTTC 5 cut(s) 964, 1797, 2483, 3957, 4916
Asp718I GGTACC 2 cut(s) 1763, 4169
AspA2I CCTAGG 1 cut(s) 1889
AspLEI GCGC 5 cut(s) 3423, 3503, 3554, 4532, 5150
AspS9I GGNCC 8 cut(s) 325, 894, 1340, 1918, 2471, 4290, 4410, 5501
AsuC2I CCSGG 2 cut(s) 2346, 5983
AsuII TTCGAA 3 cut(s) 2445, 3711, 5677
AsuNHI GCTAGC 1 cut(s) 5938
AvaI CYCGRG 1 cut(s) 2326
AvaII GGWCC 6 cut(s) 325, 894, 2471, 4290, 4410, 5501
AvrII CCTAGG 1 cut(s) 1889
AxyI CCTNAGG 1 cut(s) 5262
BaeGI GKGCMC 1 cut(s) 1182
BaeI ACNNNNGTAYC 4 cut(s) 1747, 1780, 2742, 2775
BalI TGGCCA 2 cut(s) 1484, 1559
BamHI GGATCC 3 cut(s) 706, 2269, 3032
BanI GGYRCC 3 cut(s) 1763, 4169, 4529
BanII GRGCYC 3 cut(s) 906, 2180, 2637
BbrPI CACGTG 1 cut(s) 869
BbsI GAAGAC 4 cut(s) 1088, 1566, 4233, 4353
Bbv12I GWGCWC 4 cut(s) 906, 1713, 2180, 2637
BbvCI CCTCAGC 2 cut(s) 2224, 5727
BceAI ACGGC 4 cut(s) 4353, 5096, 5161, 5618
BcgI CGANNNNNNTGC 4 cut(s) 2791, 2825, 3651, 3685
BciVI GTATCC 5 cut(s) 212, 617, 1040, 2208, 5940
BclI TGATCA 4 cut(s) 829, 1749, 3406, 5916
BcnI CCSGG 2 cut(s) 2346, 5983
BcuI ACTAGT 1 cut(s) 1730
BfoI RGCGCY 3 cut(s) 3424, 4533, 5151
BfrI CTTAAG 1 cut(s) 1922
BfuAI ACCTGC 1 cut(s) 5392
BfuI GTATCC 5 cut(s) 212, 617, 1040, 2208, 5940
BglI GCCNNNNNGGC 1 cut(s) 347
BglII AGATCT 1 cut(s) 3026
BlnI CCTAGG 1 cut(s) 1889
BlpI GCTNAGC 1 cut(s) 1448
BmcAI AGTACT 1 cut(s) 1729
Bme18I GGWCC 6 cut(s) 325, 894, 2471, 4290, 4410, 5501
BmeRI GACNNNNNGTC 1 cut(s) 1691
BmeT110I CYCGRG 1 cut(s) 2326
BmgT120I GGNCC 8 cut(s) 325, 894, 1340, 1918, 2471, 4290, 4410, 5501
BmrI ACTGGG 4 cut(s) 3113, 4024, 5281, 6030
BmtI GCTAGC 1 cut(s) 5942
BmuI ACTGGG 4 cut(s) 3113, 4024, 5281, 6030
BpiI GAAGAC 4 cut(s) 1088, 1566, 4233, 4353
BplI GAGNNNNNCTC 2 cut(s) 5720, 5752
BpmI CTGGAG 3 cut(s) 3278, 3688, 5994
Bpu10I CCTNAGC 3 cut(s) 2224, 5727, 5934
Bpu1102I GCTNAGC 1 cut(s) 1448
Bpu14I TTCGAA 3 cut(s) 2445, 3711, 5677
BpuEI CTTGAG 7 cut(s) 176, 600, 4751, 5166, 5208, 5286, 5325
BpuMI CCSGG 2 cut(s) 2346, 5983
Bsa29I ATCGAT 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 869
BsaBI GATNNNNATC 6 cut(s) 47, 361, 834, 1230, 3031, 5712
BsaHI GRCGYC 3 cut(s) 1093, 4530, 4611
BsaI GGTCTC 1 cut(s) 2026
BsaWI WCCGGW 4 cut(s) 22, 2017, 2473, 5770
BsaXI ACNNNNNCTCC 6 cut(s) 2468, 2498, 4798, 4828, 5485, 5515
Bse118I RCCGGY 4 cut(s) 22, 341, 2017, 2121
Bse21I CCTNAGG 1 cut(s) 5262
Bse3DI GCAATG 8 cut(s) 1551, 1577, 1652, 1826, 2091, 2295, 2532, 3447
Bse8I GATNNNNATC 6 cut(s) 47, 361, 834, 1230, 3031, 5712
BseAI TCCGGA 2 cut(s) 2473, 5770
BseCI ATCGAT 1 cut(s) 92
BseJI GATNNNNATC 6 cut(s) 47, 361, 834, 1230, 3031, 5712
BseMI GCAATG 8 cut(s) 1551, 1577, 1652, 1826, 2091, 2295, 2532, 3447
BseRI GAGGAG 2 cut(s) 1079, 5645
BseSI GKGCMC 1 cut(s) 1182
BseYI CCCAGC 5 cut(s) 3975, 4767, 4925, 4955, 5510
BsgI GTGCAG 3 cut(s) 369, 795, 3395
Bsh1236I CGCG 1 cut(s) 3552
BshNI GGYRCC 3 cut(s) 1763, 4169, 4529
BshTI ACCGGT 2 cut(s) 22, 2017
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 4 cut(s) 906, 1713, 2180, 2637
BsiHKCI CYCGRG 1 cut(s) 2326
BsmBI CGTCTC 2 cut(s) 2376, 5292
BsmI GAATGC 2 cut(s) 1371, 4957
Bso31I GGTCTC 1 cut(s) 2026
BsoBI CYCGRG 1 cut(s) 2326
Bsp119I TTCGAA 3 cut(s) 2445, 3711, 5677
Bsp1286I GDGCHC 5 cut(s) 906, 1182, 1713, 2180, 2637
Bsp13I TCCGGA 2 cut(s) 2473, 5770
Bsp1407I TGTACA 1 cut(s) 83
Bsp1720I GCTNAGC 1 cut(s) 1448
Bsp19I CCATGG 4 cut(s) 4361, 4481, 4562, 4643
BspDI ATCGAT 1 cut(s) 92
BspEI TCCGGA 2 cut(s) 2473, 5770
BspFNI CGCG 1 cut(s) 3552
BspMAI CTGCAG 6 cut(s) 2696, 2797, 3823, 4588, 4723, 5910
BspMI ACCTGC 1 cut(s) 5392
BspOI GCTAGC 1 cut(s) 5942
BspT104I TTCGAA 3 cut(s) 2445, 3711, 5677
BspT107I GGYRCC 3 cut(s) 1763, 4169, 4529
BspTI CTTAAG 1 cut(s) 1922
BspTNI GGTCTC 1 cut(s) 2026
BsrDI GCAATG 8 cut(s) 1551, 1577, 1652, 1826, 2091, 2295, 2532, 3447
BsrFI RCCGGY 4 cut(s) 22, 341, 2017, 2121
BsrGI TGTACA 1 cut(s) 83
BssAI RCCGGY 4 cut(s) 22, 341, 2017, 2121
BssNI GRCGYC 3 cut(s) 1093, 4530, 4611
BssT1I CCWWGG 9 cut(s) 142, 897, 1889, 4361, 4481, 4562, 4643, 4740, 4902
Bst6I CTCTTC 6 cut(s) 570, 736, 744, 982, 2453, 2584
BstACI GRCGYC 3 cut(s) 1093, 4530, 4611
BstAFI CTTAAG 1 cut(s) 1922
BstAPI GCANNNNNTGC 7 cut(s) 1122, 1285, 1642, 1658, 1828, 4592, 4790
BstAUI TGTACA 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 869
BstBI TTCGAA 3 cut(s) 2445, 3711, 5677
BstC8I GCNNGC 8 cut(s) 1281, 1466, 1659, 2123, 2790, 5208, 5906, 5940
BstDSI CCRYGG 4 cut(s) 4361, 4481, 4562, 4643
BstFNI CGCG 1 cut(s) 3552
BstH2I RGCGCY 3 cut(s) 3424, 4533, 5151
BstHHI GCGC 5 cut(s) 3423, 3503, 3554, 4532, 5150
BstNSI RCATGY 4 cut(s) 2705, 3501, 3686, 4712
BstSLI GKGCMC 1 cut(s) 1182
BstUI CGCG 1 cut(s) 3552
BstV2I GAAGAC 4 cut(s) 1088, 1566, 4233, 4353
BstX2I RGATCY 7 cut(s) 706, 1231, 2269, 2465, 2883, 3026, 3032
BstXI CCANNNNNNTGG 6 cut(s) 4164, 4203, 4323, 4362, 4443, 4482
BstYI RGATCY 7 cut(s) 706, 1231, 2269, 2465, 2883, 3026, 3032
Bsu15I ATCGAT 1 cut(s) 92
Bsu36I CCTNAGG 1 cut(s) 5262
BsuI GTATCC 5 cut(s) 212, 617, 1040, 2208, 5940
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 4 cut(s) 4361, 4481, 4562, 4643
BtgZI GCGATG 1 cut(s) 1152
BtsI GCAGTG 3 cut(s) 1283, 2375, 4716
BveI ACCTGC 1 cut(s) 5392
Cac8I GCNNGC 8 cut(s) 1281, 1466, 1659, 2123, 2790, 5208, 5906, 5940
CaiI CAGNNNCTG 1 cut(s) 671
CfoI GCGC 5 cut(s) 3423, 3503, 3554, 4532, 5150
Cfr10I RCCGGY 4 cut(s) 22, 341, 2017, 2121
Cfr13I GGNCC 8 cut(s) 325, 894, 1340, 1918, 2471, 4290, 4410, 5501
ClaI ATCGAT 1 cut(s) 92
CseI GACGC 4 cut(s) 1082, 4238, 4358, 4600
CspAI ACCGGT 2 cut(s) 22, 2017
CspCI CAANNNNNGTGG 2 cut(s) 3511, 3546
DinI GGCGCC 1 cut(s) 4531
DraI TTTAAA 1 cut(s) 2965
DraIII CACNNNGTG 1 cut(s) 1349
DrdI GACNNNNNNGTC 1 cut(s) 2977
DriI GACNNNNNGTC 1 cut(s) 1691
DseDI GACNNNNNNGTC 1 cut(s) 2977
EaeI YGGCCR 5 cut(s) 348, 1482, 1557, 3102, 3165
Eam1104I CTCTTC 6 cut(s) 570, 736, 744, 982, 2453, 2584
Eam1105I GACNNNNNGTC 1 cut(s) 1691
EarI CTCTTC 6 cut(s) 570, 736, 744, 982, 2453, 2584
EciI GGCGGA 1 cut(s) 4545
Ecl136II GAGCTC 3 cut(s) 904, 2178, 2635
Eco130I CCWWGG 9 cut(s) 142, 897, 1889, 4361, 4481, 4562, 4643, 4740, 4902
Eco147I AGGCCT 1 cut(s) 1252
Eco24I GRGCYC 3 cut(s) 906, 2180, 2637
Eco31I GGTCTC 1 cut(s) 2026
Eco47I GGWCC 6 cut(s) 325, 894, 2471, 4290, 4410, 5501
Eco53kI GAGCTC 3 cut(s) 904, 2178, 2635
Eco57I CTGAAG 5 cut(s) 290, 594, 1812, 3248, 5964
Eco72I CACGTG 1 cut(s) 869
Eco81I CCTNAGG 1 cut(s) 5262
Eco88I CYCGRG 1 cut(s) 2326
EcoICRI GAGCTC 3 cut(s) 904, 2178, 2635
EcoO109I RGGNCCY 2 cut(s) 325, 1918
EcoRI GAATTC 1 cut(s) 2231
EcoT14I CCWWGG 9 cut(s) 142, 897, 1889, 4361, 4481, 4562, 4643, 4740, 4902
EcoT22I ATGCAT 2 cut(s) 1006, 5691
EcoT38I GRGCYC 3 cut(s) 906, 2180, 2637
EgeI GGCGCC 1 cut(s) 4531
EheI GGCGCC 1 cut(s) 4531
ErhI CCWWGG 9 cut(s) 142, 897, 1889, 4361, 4481, 4562, 4643, 4740, 4902
Esp3I CGTCTC 2 cut(s) 2376, 5292
FauI CCCGC 1 cut(s) 989
FauNDI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
FbaI TGATCA 4 cut(s) 829, 1749, 3406, 5916
FblI GTMKAC 1 cut(s) 5021
FriOI GRGCYC 3 cut(s) 906, 2180, 2637
GlaI GCGC 5 cut(s) 3422, 3502, 3553, 4531, 5149
GsaI CCCAGC 5 cut(s) 3979, 4771, 4929, 4959, 5514
GsuI CTGGAG 3 cut(s) 3278, 3688, 5994
HaeII RGCGCY 3 cut(s) 3424, 4533, 5151
HgaI GACGC 4 cut(s) 1082, 4238, 4358, 4600
HhaI GCGC 5 cut(s) 3423, 3503, 3554, 4532, 5150
Hin1I GRCGYC 3 cut(s) 1093, 4530, 4611
Hin6I GCGC 5 cut(s) 3421, 3501, 3552, 4530, 5148
HinP1I GCGC 5 cut(s) 3421, 3501, 3552, 4530, 5148
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 9
Hpy166II GTNNAC 6 cut(s) 125, 2498, 3594, 3680, 3790, 5022
Hpy8I GTNNAC 6 cut(s) 125, 2498, 3594, 3680, 3790, 5022
Hpy99I CGWCG 2 cut(s) 2649, 3166
HpyCH4IV ACGT 4 cut(s) 868, 2385, 5285, 5476
HpySE526I ACGT 4 cut(s) 868, 2385, 5285, 5476
Hsp92I GRCGYC 3 cut(s) 1093, 4530, 4611
HspAI GCGC 5 cut(s) 3421, 3501, 3552, 4530, 5148
KasI GGCGCC 1 cut(s) 4529
Kpn2I TCCGGA 2 cut(s) 2473, 5770
KpnI GGTACC 2 cut(s) 1767, 4173
KroI GCCGGC 1 cut(s) 2121
KroNI GCCGGC 1 cut(s) 2123
Ksp22I TGATCA 4 cut(s) 829, 1749, 3406, 5916
MaeII ACGT 4 cut(s) 868, 2385, 5285, 5476
MaeIII GTNAC 6 cut(s) 688, 1679, 2853, 3882, 5267, 5699
MfeI CAATTG 2 cut(s) 437, 1985
MflI RGATCY 7 cut(s) 706, 1231, 2269, 2465, 2883, 3026, 3032
MhlI GDGCHC 5 cut(s) 906, 1182, 1713, 2180, 2637
MlsI TGGCCA 2 cut(s) 1484, 1559
MluNI TGGCCA 2 cut(s) 1484, 1559
Mly113I GGCGCC 1 cut(s) 4530
MlyI GAGTC 7 cut(s) 292, 2060, 2344, 2807, 4090, 4411, 5027
MmeI TCCRAC 7 cut(s) 1280, 1604, 2089, 2247, 3496, 4889, 4986
Mox20I TGGCCA 2 cut(s) 1484, 1559
Mph1103I ATGCAT 2 cut(s) 1006, 5691
MroI TCCGGA 2 cut(s) 2473, 5770
MroNI GCCGGC 1 cut(s) 2121
MroXI GAANNNNTTC 5 cut(s) 964, 1797, 2483, 3957, 4916
MscI TGGCCA 2 cut(s) 1484, 1559
Msp20I TGGCCA 2 cut(s) 1484, 1559
MspA1I CMGCKG 6 cut(s) 1444, 3623, 4055, 4583, 5423, 5726
MspCI CTTAAG 1 cut(s) 1922
MunI CAATTG 2 cut(s) 437, 1985
Mva1269I GAATGC 2 cut(s) 1371, 4957
MvnI CGCG 1 cut(s) 3552
NaeI GCCGGC 1 cut(s) 2123
NarI GGCGCC 1 cut(s) 4530
NciI CCSGG 2 cut(s) 2346, 5983
NcoI CCATGG 4 cut(s) 4361, 4481, 4562, 4643
NdeI CATATG 5 cut(s) 1036, 1950, 3358, 3636, 3783
NgoMIV GCCGGC 1 cut(s) 2121
NheI GCTAGC 1 cut(s) 5938
NmuCI GTSAC 2 cut(s) 1679, 3882
NsiI ATGCAT 2 cut(s) 1006, 5691
NspI RCATGY 4 cut(s) 2705, 3501, 3686, 4712
NspV TTCGAA 3 cut(s) 2445, 3711, 5677
PaeR7I CTCGAG 1 cut(s) 2326
PasI CCCWGGG 2 cut(s) 4122, 4242
PceI AGGCCT 1 cut(s) 1252
PciI ACATGT 2 cut(s) 3682, 4708
PcsI WCGNNNNNNNCGW 2 cut(s) 1499, 2451
PctI GAATGC 2 cut(s) 1371, 4957
PdiI GCCGGC 1 cut(s) 2123
PdmI GAANNNNTTC 5 cut(s) 964, 1797, 2483, 3957, 4916
PflFI GACNNNGTC 1 cut(s) 2387
PflMI CCANNNNNTGG 5 cut(s) 143, 419, 470, 4908, 5510
PfoI TCCNGGA 1 cut(s) 3961
PinAI ACCGGT 2 cut(s) 22, 2017
PleI GAGTC 7 cut(s) 291, 2060, 2344, 2807, 4090, 4411, 5026
PluTI GGCGCC 1 cut(s) 4533
PmaCI CACGTG 1 cut(s) 869
PmlI CACGTG 1 cut(s) 869
PpsI GAGTC 7 cut(s) 291, 2060, 2344, 2807, 4090, 4411, 5026
Ppu21I YACGTR 1 cut(s) 869
PpuMI RGGWCCY 1 cut(s) 325
PscI ACATGT 2 cut(s) 3682, 4708
PsiI TTATAA 1 cut(s) 3537
Psp124BI GAGCTC 3 cut(s) 906, 2180, 2637
Psp1406I AACGTT 1 cut(s) 5476
Psp5II RGGWCCY 1 cut(s) 325
PspCI CACGTG 1 cut(s) 869
PspFI CCCAGC 5 cut(s) 3975, 4767, 4925, 4955, 5510
PspPI GGNCC 8 cut(s) 325, 894, 1340, 1918, 2471, 4290, 4410, 5501
PspPPI RGGWCCY 1 cut(s) 325
PstI CTGCAG 6 cut(s) 2696, 2797, 3823, 4588, 4723, 5910
PstNI CAGNNNCTG 1 cut(s) 671
PsuI RGATCY 7 cut(s) 706, 1231, 2269, 2465, 2883, 3026, 3032
PsyI GACNNNGTC 1 cut(s) 2387
PvuII CAGCTG 6 cut(s) 1444, 3623, 4055, 4583, 5423, 5726
SacI GAGCTC 3 cut(s) 906, 2180, 2637
Sau96I GGNCC 8 cut(s) 325, 894, 1340, 1918, 2471, 4290, 4410, 5501
ScaI AGTACT 1 cut(s) 1729
SchI GAGTC 7 cut(s) 292, 2060, 2344, 2807, 4090, 4411, 5027
SduI GDGCHC 5 cut(s) 906, 1182, 1713, 2180, 2637
SfoI GGCGCC 1 cut(s) 4531
Sfr274I CTCGAG 1 cut(s) 2326
SfuI TTCGAA 3 cut(s) 2445, 3711, 5677
SinI GGWCC 6 cut(s) 325, 894, 2471, 4290, 4410, 5501
SlaI CTCGAG 1 cut(s) 2326
SmlI CTYRAG 9 cut(s) 155, 615, 1922, 2326, 4730, 5181, 5223, 5301, 5340
SmoI CTYRAG 9 cut(s) 155, 615, 1922, 2326, 4730, 5181, 5223, 5301, 5340
SpeI ACTAGT 1 cut(s) 1730
SseBI AGGCCT 1 cut(s) 1252
SspDI GGCGCC 1 cut(s) 4529
SspI AATATT 2 cut(s) 1087, 5963
SstI GAGCTC 3 cut(s) 906, 2180, 2637
StuI AGGCCT 1 cut(s) 1252
StyI CCWWGG 9 cut(s) 142, 897, 1889, 4361, 4481, 4562, 4643, 4740, 4902
TaiI ACGT 4 cut(s) 871, 2388, 5288, 5479
TaqII GACCGA 1 cut(s) 5987
TatI WGTACW 3 cut(s) 83, 1727, 4814
TauI GCSGC 2 cut(s) 1403, 2708
TseFI GTSAC 2 cut(s) 1679, 3882
Tsp45I GTSAC 2 cut(s) 1679, 3882
TspGWI ACGGA 2 cut(s) 4587, 4961
Tth111I GACNNNGTC 1 cut(s) 2387
Van91I CCANNNNNTGG 5 cut(s) 143, 419, 470, 4908, 5510
Vha464I CTTAAG 1 cut(s) 1922
VpaK11BI GGWCC 6 cut(s) 325, 894, 2471, 4290, 4410, 5501
XceI RCATGY 4 cut(s) 2705, 3501, 3686, 4712
XhoI CTCGAG 1 cut(s) 2326
XmaJI CCTAGG 1 cut(s) 1889
XmiI GTMKAC 1 cut(s) 5021
XmnI GAANNNNTTC 5 cut(s) 964, 1797, 2483, 3957, 4916
ZrmI AGTACT 1 cut(s) 1729
Zsp2I ATGCAT 2 cut(s) 1006, 5691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.