pycom07g02760

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006658
Physical Location & Seq
Forward (+)
2331354 .. 2338416
7063 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02760.11

Sequence Viewer

Length: 3072 bp
ATGTCCGCGGATCCTTCGATTTCAATGCTTAGAAAAGTTCTAAAGCAAATTGAAGTCATCAGAAGTTCAAGGTCGGGCATACCTAATTTTGAATCCCAAATTGTAGAAGCCAATGAACTTCAAGCAATTGTAGATCAGTATCTTCAAGTTAGGGGTACTGGGAAGGCATCACGGGACATTGATGCGAGATTAGGGGTTAATAGGGAGCTTAATGCCTCTTCAACGAAAGCTTGGCTTGAAAAAATGAGGACACTGTTCATCAGAAAGGGTTCAGGCTTCTCTTCCCGCTCAGTGATTACGGGGGATGCATATAAAAGGGTGAATGAAGTTGGCATCCCTTATGAAATTGCTCAAAGAATCACCTTTGAGGAGAAGGTTAATGACCATAACATTAGGTACCTACAACAGCTAGTAGATAAGAAGTTATGCCTAACCTATAGGGATGGTTCATCAACTTACTCATTAAGGGAAGGTTCCAAAGGGCACACATTTTTGAGGCCTGGTCAAGTGGTGCATCGGAGGATTCTGGATGGGGACCTTGTATTCGTAAACAGGCCCCCAACTACACACAAACATTCGCTACAAGCACTACAAGTGTATGTGCATGATGACCATGTGGTGAAAATAAATCCTCTGATATGTGGGCCACTAAGTGCTGACTTTGATGGTGATTGCATCCATTTGTTCTATCCGCAATCGCTTGCTGCAAAGGCAGAAGTTTTGGAGCTTTTTTCAGTGGAGAAGCAGCTGCTTAGCTCTCATAGTGGAAAGCCAAATTTGCAAATGGCCACTGATTCACTGTTGTCATTGAAGATGATGTTTAAAAACTATTTTTTGGATAAATCAGCAGCTCAGCAACTGGCCATGTTTGCTTCATCTTCGTTGCCACGACCTGCATTATTGAAAGCAAATGCTGCTCGCTCATTTTGGACTGCTTTGCAGATATTACAGACTGCATTGCCTGCTCACTTTGATTACTGTGGGGAAAAGTACTTAGTCAATAAAAGTGAGATACTGAAAATTGATTTCAGTACAAGTGAAGTTTCGGCAGTGATGAATGATGTTGCTACCTCAGTTTTCTTCGAGAAAGGTGGCGAAGATGTCCTCAAATTCTTCGATTCTCTACAGCCCTTGCTGATGGAGAACATATTTTCAGAAGGGTTCAGCGTTGGCTTGGAAGATTTTTACATGTCAAAGACATCAATACAAGATATTCAGAAGAATATTCAGGATACTTCAGATTTGTTGTATCATCTGAGGTCAACCTACAATGAATTTGTAGAGTTTCAGTTAGAGAGTCGAATCAAAAACTTGAAAGTACCAGTTTCATGTTTTATCTTGGAGTCATCTGCATTTGGTGATTTAATCGACTCCAAGAGTGATTCGGCAATCAACAAGGTTGTACAACAAATTGGGTTCCTAGGCCTACAACTCTCTGATAAGGGAAGATTCTACTCGAAGACGTTGGTTGAGGATGTGGCCTCTCTCTATCACAACAAGTATTCTTTTAACATGGAGTATCCCTCTGCAGAATATGGACTAGTTCAAAGCTGTTTCTTCCATGGGTTAGATCCATACGAGGAGATAGTTCACTCCATTGCTACACGAGAGGTGATTGTTCGGTCCTCAAGAGGATTGACCGAACCTGGGACCTTATTTAAAAATTTAATGGCCATTCTTCGGGATGTGGTTATTTGCTATGATGGCACGGTCAGAAATGTATGTAGCAATTCAGTAATCCAATTTGAGTATGGAGTCAACTCTGGATCTAGGCCCCCGAATCTGTTCCCTGCTGGTGAACCTGTTGGCGTATTAGCTGCCACTGCAATGTCAAATCCTGCGTACAAGGCAGTTCTTGATTCTACCCCAAGCAGCAATTCTTCATGGGAGCTGATGAAGGAAATTTTGCTTTGCAAAGTCAATTTTAAGAACGAGCTCATTGATCGCCGTGTAATTTTGTACTTGAGTAACTGTGGTTGTGGTAGAACATTCTGCCGAGAACGTGCAGCATGTTTGGTTAAGAATCAGTTGAAGAAAGTCAGTCTTAAAGATACTGCAGTTGAATTCATGATTGAATATAGCAATCAGCTTTCTAGTTTGGGAAGTATGGTAAATGAAGCAGGCCTAGTTGGTCATATTCATCTGAACGAGGAGCTGTTAAGGGAGTTAAATGTTGGTGTAGACGAGATTCAACAAAAATGCCAAGAAACTGTTAATTCATTTCGGAGGAAGAGAGTTGGGAAGAAAAAGTTCAACATTGGCTATCTTTTCAAAAATACAGTTTTGTTTGCCAGTGAGCATTGTTCTTTTCATCATTCCTGTGCAGACAAAAGATCTGACTCCCCATGCTTGATGTTCTTCCTTCAAGCTACTGAAGAGTTGGAGACCACTTCGCAATACTATGCTGACTTGATTTGCCCTGTGCTTTTGGAAACAATAATAAAAGGTGACCCTCGAATTTCGTCAGCAAATATAATATGGATTGATCCAGATACAACAACTTGGATTAGAAGCCCAAATAAATCTCAAAAGGGTGAATGGGCCTTGGATGTTGTTCTGGAGAAATCTGTTGTCAAGCAAAGTGGTGATGCATGGAGAATCGTTCTCGATTCTTGCCTCCCCGTGCTTCACCTAATTGACACCAGGCGTTCCATCCCATATGCCATCAAACAGATTCAAGAGCTGTTGGGAGTGTCTTGTGCTTTTGATCAAGCAGTTCAGACACAACACATTTTTGGATTGATTACACAGCGCCTCGCAACAGCTGTTACAATGGTGGCGAAAGGTGTCCTGAAAGAGCATCTTATCCTCCTGGCAAATAGCATGACATGTGCTGGAAATTTTGTTGGCTTTAATTCTAGTGGATATAAAGCACTATCTCGCGCATTGAATATTCAAGTACCATTTACAGAAGCAACACTTTTTACACCAAGAAAGTGCTTTGAGAGAGCAGCTGAAAAGTGCCACATGGATTCGTTGTCAAGCATAGTTGCATCATGTTCATGGGGTAAACATGTTGCTGTTGGTACTGGATCCAGGTTTGATATCCTGTGGGACACACGAGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

1024

Amino Acids

114.64

Weight (kDa)

7.28

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 396
AccB1I GGYRCC 1 cut(s) 396
AccBSI CCGCTC 1 cut(s) 288
AccI GTMKAC 1 cut(s) 2181
AccII CGCG 2 cut(s) 8, 2883
AciI CCGC 4 cut(s) 6, 8, 286, 692
AclWI GGATC 7 cut(s) 5, 18, 1565, 1774, 2480, 3027, 3040
AcoI YGGCCR 3 cut(s) 786, 861, 1671
AcsI RAATTY 8 cut(s) 775, 1109, 1274, 1663, 1902, 2063, 2457, 2839
AcuI CTGAAG 2 cut(s) 1221, 2394
AdeI CACNNNGTG 1 cut(s) 653
AfiI CCNNNNNNNGG 2 cut(s) 1647, 1680
AflIII ACRYGT 3 cut(s) 1188, 2828, 3013
AhlI ACTAGT 1 cut(s) 1540
AjnI CCWGG 5 cut(s) 499, 1645, 2642, 2811, 3035
AjuI GAANNNNNNNTTGG 2 cut(s) 1861, 1893
Alw21I GWGCWC 1 cut(s) 1938
Alw26I GTCTC 1 cut(s) 2378
AlwI GGATC 7 cut(s) 5, 18, 1565, 1774, 2480, 3027, 3040
AlwNI CAGNNNCTG 1 cut(s) 859
ApeKI GCWGC 9 cut(s) 704, 745, 748, 848, 914, 1817, 1872, 2006, 2951
ApoI RAATTY 8 cut(s) 775, 1109, 1274, 1663, 1902, 2063, 2457, 2839
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 3 cut(s) 268, 1784, 2249
Asp718I GGTACC 1 cut(s) 396
AspA2I CCTAGG 1 cut(s) 1420
AspLEI GCGC 2 cut(s) 2754, 2885
AspS9I GGNCC 7 cut(s) 535, 555, 644, 1623, 1650, 1773, 2541
AvaII GGWCC 3 cut(s) 535, 1623, 1650
AvrII CCTAGG 1 cut(s) 1420
BaeGI GKGCMC 1 cut(s) 486
BaeI ACNNNNGTAYC 2 cut(s) 2043, 2076
BalI TGGCCA 3 cut(s) 788, 863, 1673
BamHI GGATCC 2 cut(s) 10, 3032
BanI GGYRCC 1 cut(s) 396
BanII GRGCYC 1 cut(s) 1938
BauI CACGAG 2 cut(s) 1605, 3060
BbsI GAAGAC 1 cut(s) 1466
Bbv12I GWGCWC 1 cut(s) 1938
BbvI GCAGC 9 cut(s) 691, 735, 757, 860, 901, 1804, 1884, 2018, 2963
BccI CCATC 7 cut(s) 437, 524, 659, 1132, 1697, 2660, 2672
BceAI ACGGC 1 cut(s) 1932
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 5 cut(s) 501, 1647, 2644, 2813, 3037
BciVI GTATCC 2 cut(s) 1225, 1530
BclI TGATCA 1 cut(s) 2707
BcoDI GTCTC 1 cut(s) 2378
BcuI ACTAGT 1 cut(s) 1540
BfaI CTAG 7 cut(s) 410, 1421, 1541, 1770, 2094, 2126, 2859
BfmI CTRYAG 4 cut(s) 436, 1124, 1527, 2055
BfoI RGCGCY 1 cut(s) 2755
BfuAI ACCTGC 1 cut(s) 901
BfuI GTATCC 2 cut(s) 1225, 1530
BglII AGATCT 1 cut(s) 2333
BisI GCNGC 9 cut(s) 705, 746, 749, 849, 915, 1818, 1873, 2007, 2952
BlnI CCTAGG 1 cut(s) 1420
BlpI GCTNAGC 2 cut(s) 752, 852
BlsI GCNGC 9 cut(s) 706, 747, 750, 850, 916, 1819, 1874, 2008, 2953
BmcAI AGTACT 1 cut(s) 992
Bme1390I CCNGG 5 cut(s) 501, 1647, 2644, 2813, 3037
Bme18I GGWCC 3 cut(s) 535, 1623, 1650
BmgT120I GGNCC 7 cut(s) 535, 555, 644, 1623, 1650, 1773, 2541
BmiI GGNNCC 9 cut(s) 12, 398, 475, 536, 557, 1418, 1651, 1775, 3034
BmrFI CCNGG 5 cut(s) 501, 1647, 2644, 2813, 3037
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 9 cut(s) 172, 176, 295, 342, 523, 684, 2578, 2809, 3002
BmuI ACTGGG 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 1466
BplI GAGNNNNNCTC 2 cut(s) 1508, 1540
BpmI CTGGAG 1 cut(s) 2579
Bpu1102I GCTNAGC 2 cut(s) 752, 852
BpuEI CTTGAG 2 cut(s) 1612, 1984
BsaBI GATNNNNATC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 2378
BsaJI CCNNGG 5 cut(s) 6, 1420, 1561, 1646, 2544
Bsc4I CCNNNNNNNGG 2 cut(s) 1647, 1680
Bse1I ACTGG 5 cut(s) 163, 864, 1322, 2292, 3034
Bse3DI GCAATG 3 cut(s) 956, 1596, 1833
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 5 cut(s) 501, 1647, 2644, 2813, 3037
BseDI CCNNGG 5 cut(s) 6, 1420, 1561, 1646, 2544
BseGI GGATG 9 cut(s) 310, 333, 448, 535, 675, 1480, 1690, 2554, 2652
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 2 cut(s) 1647, 1680
BseMI GCAATG 3 cut(s) 956, 1596, 1833
BseMII CTCAG 4 cut(s) 303, 866, 1086, 1247
BseNI ACTGG 5 cut(s) 163, 864, 1322, 2292, 3034
BseRI GAGGAG 3 cut(s) 383, 1595, 2165
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 9 cut(s) 691, 735, 757, 860, 901, 1804, 1884, 2018, 2963
BsgI GTGCAG 2 cut(s) 2025, 2343
Bsh1236I CGCG 2 cut(s) 8, 2883
BshNI GGYRCC 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 1938
BslFI GGGAC 4 cut(s) 188, 548, 1663, 3068
BslI CCNNNNNNNGG 2 cut(s) 1647, 1680
BsmAI GTCTC 1 cut(s) 2378
BsmFI GGGAC 4 cut(s) 188, 548, 1663, 3068
Bso31I GGTCTC 1 cut(s) 2378
Bsp1286I GDGCHC 2 cut(s) 486, 1938
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
Bsp1720I GCTNAGC 2 cut(s) 752, 852
Bsp19I CCATGG 1 cut(s) 1561
BspACI CCGC 4 cut(s) 6, 8, 286, 692
BspCNI CTCAG 4 cut(s) 302, 865, 1085, 1248
BspFNI CGCG 2 cut(s) 8, 2883
BspHI TCATGA 1 cut(s) 2067
BspLI GGNNCC 9 cut(s) 12, 398, 475, 536, 557, 1418, 1651, 1775, 3034
BspMAI CTGCAG 2 cut(s) 1531, 2059
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 7 cut(s) 5, 18, 1565, 1774, 2480, 3027, 3040
BspT107I GGYRCC 1 cut(s) 396
BspTNI GGTCTC 1 cut(s) 2378
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 3 cut(s) 956, 1596, 1833
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 5 cut(s) 163, 864, 1322, 2292, 3034
BssECI CCNNGG 5 cut(s) 6, 1420, 1561, 1646, 2544
BssMI GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
BssSI CACGAG 2 cut(s) 1605, 3060
BssT1I CCWWGG 3 cut(s) 1420, 1561, 2544
Bst2BI CACGAG 2 cut(s) 1605, 3060
Bst2UI CCWGG 5 cut(s) 501, 1647, 2644, 2813, 3037
Bst4CI ACNGT 7 cut(s) 255, 801, 980, 1711, 1973, 2212, 2281
Bst6I CTCTTC 4 cut(s) 223, 286, 2225, 2370
BstAPI GCANNNNNTGC 2 cut(s) 914, 962
BstAUI TGTACA 1 cut(s) 1402
BstC8I GCNNGC 4 cut(s) 702, 919, 963, 2122
BstDEI CTNAG 8 cut(s) 29, 289, 650, 752, 852, 994, 1072, 1256
BstDSI CCRYGG 2 cut(s) 6, 1561
BstEII GGTNACC 1 cut(s) 2447
BstF5I GGATG 9 cut(s) 310, 333, 448, 535, 675, 1480, 1690, 2554, 2652
BstFNI CGCG 2 cut(s) 8, 2883
BstH2I RGCGCY 1 cut(s) 2755
BstHHI GCGC 2 cut(s) 2754, 2885
BstKTI GATC 9 cut(s) 13, 136, 1573, 1769, 1945, 2336, 2488, 2710, 3035
BstMAI GTCTC 1 cut(s) 2378
BstMBI GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
BstMWI GCNNNNNNNGC 8 cut(s) 710, 778, 869, 914, 962, 1704, 1823, 1847
BstNI CCWGG 5 cut(s) 501, 1647, 2644, 2813, 3037
BstNSI RCATGY 4 cut(s) 1192, 2013, 2832, 3017
BstPI GGTNACC 1 cut(s) 2447
BstSCI CCNGG 5 cut(s) 499, 1645, 2642, 2811, 3035
BstSFI CTRYAG 4 cut(s) 436, 1124, 1527, 2055
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 2 cut(s) 8, 2883
BstV1I GCAGC 9 cut(s) 691, 735, 757, 860, 901, 1804, 1884, 2018, 2963
BstV2I GAAGAC 1 cut(s) 1466
BstX2I RGATCY 5 cut(s) 10, 1570, 1766, 2333, 3032
BstYI RGATCY 5 cut(s) 10, 1570, 1766, 2333, 3032
BsuI GTATCC 2 cut(s) 1225, 1530
BtgI CCRYGG 2 cut(s) 6, 1561
BtsCI GGATG 9 cut(s) 310, 333, 448, 535, 675, 1480, 1690, 2554, 2652
BtsI GCAGTG 2 cut(s) 1056, 1821
BtsIMutI CAGTG 8 cut(s) 251, 297, 741, 789, 797, 1056, 1821, 2299
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 4 cut(s) 702, 919, 963, 2122
CaiI CAGNNNCTG 1 cut(s) 859
CciI TCATGA 1 cut(s) 2067
CfoI GCGC 2 cut(s) 2754, 2885
Cfr13I GGNCC 7 cut(s) 535, 555, 644, 1623, 1650, 1773, 2541
Cfr42I CCGCGG 1 cut(s) 9
CspCI CAANNNNNGTGG 2 cut(s) 2563, 2598
DdeI CTNAG 8 cut(s) 29, 289, 650, 752, 852, 994, 1072, 1256
DpnI GATC 9 cut(s) 12, 135, 1572, 1768, 1944, 2335, 2487, 2709, 3034
DpnII GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
DraI TTTAAA 2 cut(s) 823, 1660
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 3 cut(s) 786, 861, 1671
Eam1104I CTCTTC 4 cut(s) 223, 286, 2225, 2370
EarI CTCTTC 4 cut(s) 223, 286, 2225, 2370
Ecl136II GAGCTC 1 cut(s) 1936
Eco130I CCWWGG 3 cut(s) 1420, 1561, 2544
Eco147I AGGCCT 3 cut(s) 499, 1425, 2124
Eco24I GRGCYC 1 cut(s) 1938
Eco31I GGTCTC 1 cut(s) 2378
Eco32I GATATC 1 cut(s) 3046
Eco47I GGWCC 3 cut(s) 535, 1623, 1650
Eco53kI GAGCTC 1 cut(s) 1936
Eco57I CTGAAG 2 cut(s) 1221, 2394
Eco91I GGTNACC 1 cut(s) 2447
EcoICRI GAGCTC 1 cut(s) 1936
EcoO109I RGGNCCY 4 cut(s) 535, 555, 1650, 1773
EcoO65I GGTNACC 1 cut(s) 2447
EcoRI GAATTC 1 cut(s) 2063
EcoRII CCWGG 5 cut(s) 499, 1645, 2642, 2811, 3035
EcoRV GATATC 1 cut(s) 3046
EcoT14I CCWWGG 3 cut(s) 1420, 1561, 2544
EcoT22I ATGCAT 2 cut(s) 310, 2593
EcoT38I GRGCYC 1 cut(s) 1938
ErhI CCWWGG 3 cut(s) 1420, 1561, 2544
FalI AAGNNNNNCTT 8 cut(s) 219, 251, 2028, 2060, 2787, 2819, 2904, 2936
FaqI GGGAC 4 cut(s) 188, 548, 1663, 3068
FauI CCCGC 1 cut(s) 293
FauNDI CATATG 1 cut(s) 2659
FbaI TGATCA 1 cut(s) 2707
FblI GTMKAC 1 cut(s) 2181
Fnu4HI GCNGC 9 cut(s) 705, 746, 749, 849, 915, 1818, 1873, 2007, 2952
FokI GGATG 9 cut(s) 317, 320, 455, 542, 662, 1487, 1697, 2561, 2639
FriOI GRGCYC 1 cut(s) 1938
Fsp4HI GCNGC 9 cut(s) 705, 746, 749, 849, 915, 1818, 1873, 2007, 2952
FspBI CTAG 7 cut(s) 410, 1421, 1541, 1770, 2094, 2126, 2859
GlaI GCGC 2 cut(s) 2753, 2884
GluI GCNGC 9 cut(s) 705, 746, 749, 849, 915, 1818, 1873, 2007, 2952
GsuI CTGGAG 1 cut(s) 2579
HaeII RGCGCY 1 cut(s) 2755
HhaI GCGC 2 cut(s) 2754, 2885
Hin6I GCGC 2 cut(s) 2752, 2883
HinP1I GCGC 2 cut(s) 2752, 2883
HincII GTYRAC 2 cut(s) 1263, 1759
HindII GTYRAC 2 cut(s) 1263, 1759
HindIII AAGCTT 1 cut(s) 228
Hpy166II GTNNAC 7 cut(s) 550, 1263, 1591, 1759, 1799, 2182, 3011
Hpy8I GTNNAC 7 cut(s) 550, 1263, 1591, 1759, 1799, 2182, 3011
HpyAV CCTTC 7 cut(s) 24, 157, 367, 464, 1151, 1891, 2372
HpyCH4III ACNGT 7 cut(s) 255, 801, 980, 1711, 1973, 2212, 2281
HpyCH4IV ACGT 2 cut(s) 1463, 2002
HpyF10VI GCNNNNNNNGC 8 cut(s) 710, 778, 869, 914, 962, 1704, 1823, 1847
HpyF3I CTNAG 8 cut(s) 29, 289, 650, 752, 852, 994, 1072, 1256
HpySE526I ACGT 2 cut(s) 1463, 2002
HspAI GCGC 2 cut(s) 2752, 2883
KpnI GGTACC 1 cut(s) 400
Ksp22I TGATCA 1 cut(s) 2707
KspI CCGCGG 1 cut(s) 9
Kzo9I GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
LmnI GCTCC 4 cut(s) 205, 724, 1888, 2152
Lsp1109I GCAGC 9 cut(s) 691, 735, 757, 860, 901, 1804, 1884, 2018, 2963
LweI GCATC 9 cut(s) 172, 176, 295, 342, 523, 684, 2578, 2809, 3002
MaeI CTAG 7 cut(s) 410, 1421, 1541, 1770, 2094, 2126, 2859
MaeII ACGT 2 cut(s) 1463, 2002
MaeIII GTNAC 3 cut(s) 1967, 2447, 2767
MalI GATC 9 cut(s) 12, 135, 1572, 1768, 1944, 2335, 2487, 2709, 3034
MbiI CCGCTC 1 cut(s) 288
MboI GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 5 cut(s) 10, 1570, 1766, 2333, 3032
MhlI GDGCHC 2 cut(s) 486, 1938
MlsI TGGCCA 3 cut(s) 788, 863, 1673
MluNI TGGCCA 3 cut(s) 788, 863, 1673
MlyI GAGTC 5 cut(s) 1306, 1352, 1364, 1764, 2333
MmeI TCCRAC 1 cut(s) 2361
Mox20I TGGCCA 3 cut(s) 788, 863, 1673
Mph1103I ATGCAT 2 cut(s) 310, 2593
MroXI GAANNNNTTC 3 cut(s) 268, 1784, 2249
MscI TGGCCA 3 cut(s) 788, 863, 1673
MslI CAYNNNNRTG 3 cut(s) 1826, 2319, 3001
Msp20I TGGCCA 3 cut(s) 788, 863, 1673
MspA1I CMGCKG 4 cut(s) 8, 748, 2765, 2954
MspR9I CCNGG 5 cut(s) 501, 1647, 2644, 2813, 3037
MunI CAATTG 1 cut(s) 126
MvaI CCWGG 5 cut(s) 501, 1647, 2644, 2813, 3037
MvnI CGCG 2 cut(s) 8, 2883
MwoI GCNNNNNNNGC 8 cut(s) 710, 778, 869, 914, 962, 1704, 1823, 1847
NcoI CCATGG 1 cut(s) 1561
NdeI CATATG 1 cut(s) 2659
NdeII GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
NlaIV GGNNCC 9 cut(s) 12, 398, 475, 536, 557, 1418, 1651, 1775, 3034
NmeAIII GCCGAG 1 cut(s) 2021
NmuCI GTSAC 1 cut(s) 2447
NsiI ATGCAT 2 cut(s) 310, 2593
NspI RCATGY 4 cut(s) 1192, 2013, 2832, 3017
PagI TCATGA 1 cut(s) 2067
PceI AGGCCT 3 cut(s) 499, 1425, 2124
PciI ACATGT 3 cut(s) 1188, 2828, 3013
PdmI GAANNNNTTC 3 cut(s) 268, 1784, 2249
PkrI GCNGC 9 cut(s) 706, 747, 750, 850, 916, 1819, 1874, 2008, 2953
PleI GAGTC 5 cut(s) 1305, 1351, 1364, 1763, 2333
PpsI GAGTC 5 cut(s) 1305, 1351, 1364, 1763, 2333
PpuMI RGGWCCY 2 cut(s) 535, 1650
PscI ACATGT 3 cut(s) 1188, 2828, 3013
Psp124BI GAGCTC 1 cut(s) 1938
Psp5II RGGWCCY 2 cut(s) 535, 1650
Psp6I CCWGG 5 cut(s) 499, 1645, 2642, 2811, 3035
PspEI GGTNACC 1 cut(s) 2447
PspGI CCWGG 5 cut(s) 499, 1645, 2642, 2811, 3035
PspN4I GGNNCC 9 cut(s) 12, 398, 475, 536, 557, 1418, 1651, 1775, 3034
PspPI GGNCC 7 cut(s) 535, 555, 644, 1623, 1650, 1773, 2541
PspPPI RGGWCCY 2 cut(s) 535, 1650
PstI CTGCAG 2 cut(s) 1531, 2059
PstNI CAGNNNCTG 1 cut(s) 859
PsuI RGATCY 5 cut(s) 10, 1570, 1766, 2333, 3032
PvuII CAGCTG 3 cut(s) 748, 2765, 2954
RseI CAYNNNNRTG 3 cut(s) 1826, 2319, 3001
SacI GAGCTC 1 cut(s) 1938
SacII CCGCGG 1 cut(s) 9
SatI GCNGC 9 cut(s) 705, 746, 749, 849, 915, 1818, 1873, 2007, 2952
Sau3AI GATC 9 cut(s) 10, 133, 1570, 1766, 1942, 2333, 2485, 2707, 3032
Sau96I GGNCC 7 cut(s) 535, 555, 644, 1623, 1650, 1773, 2541
ScaI AGTACT 1 cut(s) 992
SchI GAGTC 5 cut(s) 1306, 1352, 1364, 1764, 2333
ScrFI CCNGG 5 cut(s) 501, 1647, 2644, 2813, 3037
SduI GDGCHC 2 cut(s) 486, 1938
SfaNI GCATC 9 cut(s) 172, 176, 295, 342, 523, 684, 2578, 2809, 3002
SfcI CTRYAG 4 cut(s) 436, 1124, 1527, 2055
Sfr303I CCGCGG 1 cut(s) 9
SgrBI CCGCGG 1 cut(s) 9
SinI GGWCC 3 cut(s) 535, 1623, 1650
SmiMI CAYNNNNRTG 3 cut(s) 1826, 2319, 3001
SmlI CTYRAG 2 cut(s) 1627, 1963
SmoI CTYRAG 2 cut(s) 1627, 1963
SpeI ACTAGT 1 cut(s) 1540
SseBI AGGCCT 3 cut(s) 499, 1425, 2124
SsiI CCGC 4 cut(s) 6, 8, 286, 692
SspI AATATT 2 cut(s) 1225, 2893
SspMI CTAG 7 cut(s) 410, 1421, 1541, 1770, 2094, 2126, 2859
SstI GAGCTC 1 cut(s) 1938
StuI AGGCCT 3 cut(s) 499, 1425, 2124
StyD4I CCNGG 5 cut(s) 499, 1645, 2642, 2811, 3035
StyI CCWWGG 3 cut(s) 1420, 1561, 2544
TaaI ACNGT 7 cut(s) 255, 801, 980, 1711, 1973, 2212, 2281
TaiI ACGT 2 cut(s) 1466, 2005
TaqI TCGA 8 cut(s) 17, 1083, 1116, 1300, 1368, 1457, 2455, 2607
TaqII GACCGA 2 cut(s) 1611, 1655
TatI WGTACW 4 cut(s) 990, 1031, 1402, 1959
TscAI CASTG 8 cut(s) 258, 297, 741, 796, 804, 1056, 1828, 2299
TseFI GTSAC 1 cut(s) 2447
TseI GCWGC 9 cut(s) 704, 745, 748, 848, 914, 1817, 1872, 2006, 2951
Tsp45I GTSAC 1 cut(s) 2447
TspRI CASTG 8 cut(s) 258, 297, 741, 796, 804, 1056, 1828, 2299
VpaK11BI GGWCC 3 cut(s) 535, 1623, 1650
XapI RAATTY 8 cut(s) 775, 1109, 1274, 1663, 1902, 2063, 2457, 2839
XceI RCATGY 4 cut(s) 1192, 2013, 2832, 3017
XcmI CCANNNNNNNNNTGG 1 cut(s) 1748
XmaJI CCTAGG 1 cut(s) 1420
XmiI GTMKAC 1 cut(s) 2181
XmnI GAANNNNTTC 3 cut(s) 268, 1784, 2249
XspI CTAG 7 cut(s) 410, 1421, 1541, 1770, 2094, 2126, 2859
ZrmI AGTACT 1 cut(s) 992
Zsp2I ATGCAT 2 cut(s) 310, 2593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.