AT2G43340

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001712
Physical Location & Seq
Forward (+)
18007629 .. 18008665
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43340.1

Sequence Viewer

Length: 570 bp
ATGTGCGATTCAGATTTTCTCTCTCGTTGCTCTTCCTACGAAGCAGACGTTGATTCCGACAGCGAGATCTCGACCTCCAGTTTCAGTTCTTGTTCGTACAGTGAATCTGAGGAAGAAGAGATCAACAATGGTTTTGGTGTTGTTCAGTCGAAGAAGCAAACGAAGAAACTGGAGAAGAAGAAGAGTAATGTGTTGTTAGAAGGTTACGTTGTAGATTCAGCTGTGAATGATGATTTGAAGAGAACCAAGAGTTTAACGGACGATGATCTCGAGGAGCTTAAGGGATGTGTAGATCTAGGGTTTGGGTTTAATTACGAGGAGATTCCTGAACTTTGTAATACTTTACCAGCTCTTGAGCTCTGTTACTCGATGAGTCAGAAGTTTATTGATCAAGATCATCATCACCATTCTTCTTCTTCGCCAGAGAAGAAATCATCGGTTCTTGATTCTCCTGTTAGTCCTATTGCTAGCTGGAAGATCTCTAGTCCTGGTGATAATCCTGATGATGTTAAAGCAAGGTTGAAATTTTGGGCACAAGCTGTGGCATGTACTGTGAGGTTATGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

189

Amino Acids

20.96

Weight (kDa)

4.63

Isoelectric Point (pI)

61.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 85 - 140 2e-26 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 524
AfaI GTAC 3 cut(s) 98, 550, 565
AflII CTTAAG 1 cut(s) 278
AgsI TTSAA 2 cut(s) 238, 523
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 6 cut(s) 221, 277, 350, 358, 471, 539
AluI AGCT 6 cut(s) 221, 277, 350, 358, 471, 539
Alw21I GWGCWC 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 269
ApoI RAATTY 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 395, 503
AsuNHI GCTAGC 1 cut(s) 467
AvaI CYCGRG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 535
BanII GRGCYC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 360
BciT130I CCWGG 1 cut(s) 489
BclI TGATCA 1 cut(s) 388
BfaI CTAG 3 cut(s) 296, 468, 483
BfrI CTTAAG 1 cut(s) 278
BglII AGATCT 3 cut(s) 66, 292, 477
Bme1390I CCNGG 1 cut(s) 489
BmeT110I CYCGRG 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 489
BmtI GCTAGC 1 cut(s) 471
BpmI CTGGAG 2 cut(s) 61, 191
BpuEI CTTGAG 1 cut(s) 374
BsaBI GATNNNNATC 2 cut(s) 393, 399
Bse1I ACTGG 2 cut(s) 78, 174
Bse8I GATNNNNATC 2 cut(s) 393, 399
BseBI CCWGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 290
BseJI GATNNNNATC 2 cut(s) 393, 399
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 2 cut(s) 78, 174
BseRI GAGGAG 2 cut(s) 287, 332
BseSI GKGCMC 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 360, 535
Bsp143I GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
BspCNI CTCAG 1 cut(s) 100
BspOI GCTAGC 1 cut(s) 471
BspQI GCTCTTC 1 cut(s) 37
BspTI CTTAAG 1 cut(s) 278
BsrI ACTGG 2 cut(s) 78, 174
BssMI GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 2 cut(s) 101, 553
Bst6I CTCTTC 4 cut(s) 37, 111, 176, 233
BstAFI CTTAAG 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 469
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 290
BstKTI GATC 7 cut(s) 69, 123, 268, 295, 391, 397, 480
BstMBI GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 549
BstSCI CCNGG 1 cut(s) 487
BstSLI GKGCMC 1 cut(s) 535
BstX2I RGATCY 3 cut(s) 66, 292, 477
BstYI RGATCY 3 cut(s) 66, 292, 477
BtsCI GGATG 1 cut(s) 290
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 469
Csp6I GTAC 3 cut(s) 97, 549, 564
CviAII CATG 1 cut(s) 546
CviJI RGCY 6 cut(s) 221, 277, 350, 358, 471, 539
CviKI_1 RGCY 6 cut(s) 221, 277, 350, 358, 471, 539
CviQI GTAC 3 cut(s) 97, 549, 564
DdeI CTNAG 1 cut(s) 108
DpnI GATC 7 cut(s) 68, 122, 267, 294, 390, 396, 479
DpnII GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
Eam1104I CTCTTC 4 cut(s) 37, 111, 176, 233
EarI CTCTTC 4 cut(s) 37, 111, 176, 233
Ecl136II GAGCTC 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 360
Eco53kI GAGCTC 1 cut(s) 358
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 360
FaeI CATG 1 cut(s) 549
FaiI YATR 2 cut(s) 547, 562
FatI CATG 1 cut(s) 545
FbaI TGATCA 1 cut(s) 388
FokI GGATG 1 cut(s) 297
FriOI GRGCYC 1 cut(s) 360
FspBI CTAG 3 cut(s) 296, 468, 483
GsuI CTGGAG 2 cut(s) 61, 191
Hin1II CATG 1 cut(s) 549
HinfI GANTC 7 cut(s) 8, 53, 104, 215, 322, 373, 446
HphI GGTGA 2 cut(s) 395, 503
Hpy188I TCNGA 4 cut(s) 13, 58, 109, 378
Hpy188III TCNNGA 7 cut(s) 70, 269, 326, 353, 392, 443, 500
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 2 cut(s) 101, 553
HpyCH4IV ACGT 2 cut(s) 48, 207
HpyF3I CTNAG 1 cut(s) 108
HpySE526I ACGT 2 cut(s) 48, 207
Hsp92II CATG 1 cut(s) 549
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
LguI GCTCTTC 1 cut(s) 37
LmnI GCTCC 1 cut(s) 274
MaeI CTAG 3 cut(s) 296, 468, 483
MaeII ACGT 2 cut(s) 48, 207
MaeIII GTNAC 2 cut(s) 203, 362
MalI GATC 7 cut(s) 68, 122, 267, 294, 390, 396, 479
MboI GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
MflI RGATCY 3 cut(s) 66, 292, 477
MhlI GDGCHC 2 cut(s) 360, 535
MluCI AATT 2 cut(s) 310, 524
MlyI GAGTC 1 cut(s) 382
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 5 cut(s) 85, 103, 265, 310, 549
MseI TTAA 4 cut(s) 254, 279, 309, 510
MspA1I CMGCKG 1 cut(s) 221
MspCI CTTAAG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 489
NdeII GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 1 cut(s) 549
NspI RCATGY 1 cut(s) 549
PaeR7I CTCGAG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 3 cut(s) 45, 54, 267
PfeI GAWTC 6 cut(s) 8, 53, 104, 215, 322, 446
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PsuI RGATCY 3 cut(s) 66, 292, 477
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 3 cut(s) 98, 550, 565
RsaNI GTAC 3 cut(s) 97, 549, 564
SacI GAGCTC 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 37
SaqAI TTAA 4 cut(s) 254, 279, 309, 510
Sau3AI GATC 7 cut(s) 66, 120, 265, 292, 388, 394, 477
SchI GAGTC 1 cut(s) 382
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 2 cut(s) 360, 535
Sfr274I CTCGAG 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 269
SmlI CTYRAG 3 cut(s) 269, 278, 353
SmoI CTYRAG 3 cut(s) 269, 278, 353
Sse9I AATT 2 cut(s) 310, 524
SspMI CTAG 3 cut(s) 296, 468, 483
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 2 cut(s) 101, 553
TaiI ACGT 2 cut(s) 51, 210
TaqI TCGA 4 cut(s) 71, 149, 270, 368
TasI AATT 2 cut(s) 310, 524
TatI WGTACW 2 cut(s) 548, 563
TfiI GAWTC 6 cut(s) 8, 53, 104, 215, 322, 446
Tru1I TTAA 4 cut(s) 254, 279, 309, 510
Tru9I TTAA 4 cut(s) 254, 279, 309, 510
TscAI CASTG 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 1 cut(s) 106
Vha464I CTTAAG 1 cut(s) 278
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 549
XhoI CTCGAG 1 cut(s) 269
XspI CTAG 3 cut(s) 296, 468, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.