Rorug06G0154700

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001712
Physical Location & Seq
Reverse (-)
22209737 .. 22210545
809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0154700.1

Sequence Viewer

Length: 597 bp
ATGGAGCTACAAACCCAGCAACAAATCGAGGATGGAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACAGATTTCTGGCGAAGCCGTGCCAGGTGGGTTCCTACTCCAGATCAAATAAGGATCCTCAGGGATCTTTACTACGACAAGGGAGTTAAGACCCCAACTACAGAGCAGATTCACGAGATCTGTCTCCAGCTGCAACAGTATGGACAGGTTGAGGGCAAGAACATTTATTTTTGGTTCCAGAATGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGGTTGAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGGACGTCTTTGGTAACCCGAAGACTACTTCTGAGGAAGGTAGCGCATTTGGTTACTATTCTGGTGGCTCAGGCGGTTACAACAGTGGCTCTAACGTTTCTCTTGAGCTCAGCCTCAACCCATCCGGAGCTACCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

198

Amino Acids

21.46

Weight (kDa)

5.19

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 30 - 91 4.1e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 465
AccIII TCCGGA 1 cut(s) 581
AciI CCGC 1 cut(s) 531
AclI AACGTT 1 cut(s) 552
AclWI GGATC 5 cut(s) 118, 131, 141, 330, 343
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 1 cut(s) 328
AfiI CCNNNNNNNGG 2 cut(s) 329, 452
AgsI TTSAA 1 cut(s) 428
AhlI ACTAGT 1 cut(s) 314
AjnI CCWGG 3 cut(s) 92, 322, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 304, 336
AluBI AGCT 4 cut(s) 7, 199, 565, 587
AluI AGCT 4 cut(s) 7, 199, 565, 587
Alw21I GWGCWC 2 cut(s) 453, 567
Alw26I GTCTC 1 cut(s) 197
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 5 cut(s) 118, 131, 141, 330, 343
Ama87I CYCGRG 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 581
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 3 cut(s) 50, 199, 293
Asp700I GAANNNNTTC 2 cut(s) 316, 432
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 357
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 2 cut(s) 306, 453
BamHI GGATCC 2 cut(s) 123, 335
BanII GRGCYC 2 cut(s) 262, 567
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 2 cut(s) 453, 567
BbvI GCAGC 3 cut(s) 62, 186, 280
BccI CCATC 2 cut(s) 26, 586
BceAI ACGGC 2 cut(s) 72, 469
BciT130I CCWGG 3 cut(s) 94, 324, 419
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 168
BglII AGATCT 1 cut(s) 186
BisI GCNGC 3 cut(s) 51, 200, 294
BlpI GCTNAGC 1 cut(s) 566
BlsI GCNGC 3 cut(s) 52, 201, 295
Bme1390I CCNGG 4 cut(s) 55, 94, 324, 419
Bme18I GGWCC 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 5 cut(s) 102, 125, 245, 337, 358
BmrFI CCNGG 4 cut(s) 55, 94, 324, 419
BpiI GAAGAC 1 cut(s) 485
BpmI CTGGAG 3 cut(s) 93, 179, 399
Bpu10I CCTNAGC 1 cut(s) 526
Bpu1102I GCTNAGC 1 cut(s) 566
BpuEI CTTGAG 2 cut(s) 282, 581
BpuMI CCSGG 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 306
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 452
Bse1I ACTGG 3 cut(s) 334, 367, 376
Bse21I CCTNAGG 1 cut(s) 128
BseAI TCCGGA 1 cut(s) 581
BseBI CCWGG 3 cut(s) 94, 324, 419
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 3 cut(s) 37, 389, 578
BseLI CCNNNNNNNGG 2 cut(s) 329, 452
BseMII CTCAG 4 cut(s) 142, 480, 540, 580
BseNI ACTGG 3 cut(s) 334, 367, 376
BseRI GAGGAG 2 cut(s) 420, 423
BseSI GKGCMC 2 cut(s) 306, 453
BseXI GCAGC 3 cut(s) 62, 186, 280
BseYI CCCAGC 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 453, 567
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 54, 582
BslI CCNNNNNNNGG 2 cut(s) 329, 452
BsmAI GTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 4 cut(s) 262, 306, 453, 567
Bsp13I TCCGGA 1 cut(s) 581
Bsp143I GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
Bsp1720I GCTNAGC 1 cut(s) 566
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 1 cut(s) 531
BspCNI CTCAG 4 cut(s) 141, 481, 539, 579
BspEI TCCGGA 1 cut(s) 581
BspLI GGNNCC 5 cut(s) 102, 125, 245, 337, 358
BspPI GGATC 5 cut(s) 118, 131, 141, 330, 343
BsrI ACTGG 3 cut(s) 334, 367, 376
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
BssNI GRCGYC 1 cut(s) 462
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 3 cut(s) 94, 324, 419
Bst4CI ACNGT 2 cut(s) 207, 542
Bst6I CTCTTC 2 cut(s) 272, 438
BstACI GRCGYC 1 cut(s) 462
BstDEI CTNAG 4 cut(s) 128, 489, 526, 566
BstDSI CCRYGG 1 cut(s) 306
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 3 cut(s) 37, 389, 578
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 7 cut(s) 115, 126, 136, 189, 338, 346, 415
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
BstNI CCWGG 3 cut(s) 94, 324, 419
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 4 cut(s) 53, 92, 322, 417
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 2 cut(s) 306, 453
BstV1I GCAGC 3 cut(s) 62, 186, 280
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 4 cut(s) 123, 133, 186, 335
BstYI RGATCY 4 cut(s) 123, 133, 186, 335
Bsu36I CCTNAGG 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 3 cut(s) 37, 389, 578
BtsIMutI CAGTG 2 cut(s) 360, 547
CfoI GCGC 1 cut(s) 503
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 1 cut(s) 327
CviAII CATG 1 cut(s) 307
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 4 cut(s) 128, 489, 526, 566
DpnI GATC 7 cut(s) 114, 125, 135, 188, 337, 345, 414
DpnII GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
Eam1104I CTCTTC 2 cut(s) 272, 438
EarI CTCTTC 2 cut(s) 272, 438
Ecl136II GAGCTC 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 2 cut(s) 262, 567
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 565
Eco81I CCTNAGG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 565
EcoO65I GGTNACC 1 cut(s) 470
EcoRII CCWGG 3 cut(s) 92, 322, 417
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 2 cut(s) 262, 567
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 310
FaiI YATR 2 cut(s) 210, 308
FatI CATG 1 cut(s) 306
Fnu4HI GCNGC 3 cut(s) 51, 200, 294
FokI GGATG 3 cut(s) 44, 376, 565
FriOI GRGCYC 2 cut(s) 262, 567
Fsp4HI GCNGC 3 cut(s) 51, 200, 294
FspBI CTAG 1 cut(s) 315
GlaI GCGC 1 cut(s) 502
GluI GCNGC 3 cut(s) 51, 200, 294
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 3 cut(s) 93, 179, 399
HapII CCGG 2 cut(s) 54, 582
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 1 cut(s) 310
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HinfI GANTC 1 cut(s) 178
HpaII CCGG 2 cut(s) 54, 582
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 2 cut(s) 360, 451
Hpy188I TCNGA 1 cut(s) 490
Hpy188III TCNNGA 6 cut(s) 110, 182, 247, 263, 560, 582
Hpy8I GTNNAC 2 cut(s) 360, 451
HpyAV CCTTC 1 cut(s) 488
HpyCH4III ACNGT 2 cut(s) 207, 542
HpyCH4IV ACGT 2 cut(s) 462, 552
HpyCH4V TGCA 4 cut(s) 202, 285, 397, 451
HpyF3I CTNAG 4 cut(s) 128, 489, 526, 566
HpySE526I ACGT 2 cut(s) 462, 552
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 1 cut(s) 310
HspAI GCGC 1 cut(s) 501
Kpn2I TCCGGA 1 cut(s) 581
Kzo9I GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
LmnI GCTCC 2 cut(s) 4, 584
Lsp1109I GCAGC 3 cut(s) 62, 186, 280
MaeI CTAG 1 cut(s) 315
MaeII ACGT 2 cut(s) 462, 552
MaeIII GTNAC 3 cut(s) 470, 509, 533
MalI GATC 7 cut(s) 114, 125, 135, 188, 337, 345, 414
MboI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
MboII GAAGA 3 cut(s) 289, 425, 490
MflI RGATCY 4 cut(s) 123, 133, 186, 335
MhlI GDGCHC 4 cut(s) 262, 306, 453, 567
MluCI AATT 2 cut(s) 349, 592
MroI TCCGGA 1 cut(s) 581
MroXI GAANNNNTTC 2 cut(s) 316, 432
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 2 cut(s) 54, 582
MspR9I CCNGG 4 cut(s) 55, 94, 324, 419
MvaI CCWGG 3 cut(s) 94, 324, 419
NciI CCSGG 1 cut(s) 55
NcoI CCATGG 1 cut(s) 306
NdeII GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 5 cut(s) 102, 125, 245, 337, 358
PaeR7I CTCGAG 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PdmI GAANNNNTTC 2 cut(s) 316, 432
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 3 cut(s) 52, 201, 295
Psp124BI GAGCTC 1 cut(s) 567
Psp1406I AACGTT 1 cut(s) 552
Psp6I CCWGG 3 cut(s) 92, 322, 417
PspEI GGTNACC 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 3 cut(s) 92, 322, 417
PspN4I GGNNCC 5 cut(s) 102, 125, 245, 337, 358
PspPI GGNCC 1 cut(s) 357
PsuI RGATCY 4 cut(s) 123, 133, 186, 335
PvuII CAGCTG 1 cut(s) 199
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
SacI GAGCTC 1 cut(s) 567
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 3 cut(s) 51, 200, 294
Sau3AI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 412
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 4 cut(s) 55, 94, 324, 419
SduI GDGCHC 4 cut(s) 262, 306, 453, 567
SfcI CTRYAG 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 3 cut(s) 261, 297, 560
SmoI CTYRAG 3 cut(s) 261, 297, 560
SpeI ACTAGT 1 cut(s) 314
Sse9I AATT 2 cut(s) 349, 592
SsiI CCGC 1 cut(s) 531
SspMI CTAG 1 cut(s) 315
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 4 cut(s) 53, 92, 322, 417
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 207, 542
TaiI ACGT 2 cut(s) 465, 555
TaqI TCGA 2 cut(s) 27, 262
TasI AATT 2 cut(s) 349, 592
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 367, 547
TseI GCWGC 3 cut(s) 50, 199, 293
TspDTI ATGAA 1 cut(s) 290
TspRI CASTG 2 cut(s) 367, 547
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 357
XhoI CTCGAG 1 cut(s) 261
XmnI GAANNNNTTC 2 cut(s) 316, 432
XspI CTAG 1 cut(s) 315
ZraI GACGTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.