Rh4DG137500

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001712
Physical Location & Seq
Forward (+)
27388354 .. 27394507
6154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG137500.1

Sequence Viewer

Length: 486 bp
ATGGCAGCCTACATCGATTCTTGGGTTTCTTCTTCAATCCAAGAAAACCCAAATGAAGAGGAAGCAAATGAGTCATCATTGGAATCAATCTTTTCTTCTTCTGCGTTCAACCGACAAGTGGAGCGGGAGGAAGGAGAATTGCCCAAGCTAAGGAGCTCATCGTCGAGCGGCGGCAGGAAGAAGAAGAAGAAGAACCAGGTTTTGCTTGAAGGGTACGTTGATTCGGCCGATGAGGACGATCTGTCGAGGACCAAGAGCTTGACGGATGAGGATCTCGATGAGCTCAAAGGATGCTTGGATCTGGGATTCGGGTTCAGCTATGAAGAAATTCCCGAGCTCTGTAACACCTTGCCTGCTCTTGAGCTTTGTTACTCTATGAGCCAGAAGTTTATGGATAAGTCGCCGGACAGCTCTCCTGCTGCACTGGATTCCTGCTCTTCTGTGTCCAGTCCGATTGCCAATTGGAAGATCTCCAGTCCTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

161

Amino Acids

17.65

Weight (kDa)

4.36

Isoelectric Point (pI)

67.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 87 - 140 6.9e-25 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 124, 168
AciI CCGC 3 cut(s) 124, 168, 171
AclWI GGATC 2 cut(s) 279, 306
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 1 cut(s) 327
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 118, 150
AgsI TTSAA 3 cut(s) 36, 109, 209
AhdI GACNNNNNGTC 1 cut(s) 241
AjnI CCWGG 2 cut(s) 195, 478
AluBI AGCT 8 cut(s) 148, 156, 258, 283, 318, 337, 364, 411
AluI AGCT 8 cut(s) 148, 156, 258, 283, 318, 337, 364, 411
Alw21I GWGCWC 3 cut(s) 158, 285, 339
AlwI GGATC 2 cut(s) 279, 306
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 1 cut(s) 225
ApeKI GCWGC 2 cut(s) 5, 419
ApoI RAATTY 1 cut(s) 327
Asp700I GAANNNNTTC 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 249
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 1 cut(s) 249
BanII GRGCYC 3 cut(s) 158, 285, 339
Bbv12I GWGCWC 3 cut(s) 158, 285, 339
BbvI GCAGC 2 cut(s) 17, 406
BciT130I CCWGG 2 cut(s) 197, 480
BglII AGATCT 1 cut(s) 468
BisI GCNGC 4 cut(s) 6, 169, 172, 420
BlsI GCNGC 4 cut(s) 7, 170, 173, 421
Bme1390I CCNGG 2 cut(s) 197, 480
Bme18I GGWCC 1 cut(s) 249
BmeRI GACNNNNNGTC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 249
BmrFI CCNGG 2 cut(s) 197, 480
BmsI GCATC 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 457
Bpu10I CCTNAGC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 380
Bsa29I ATCGAT 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 270
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 150
Bse1I ACTGG 3 cut(s) 429, 447, 474
Bse8I GATNNNNATC 1 cut(s) 270
BseBI CCWGG 2 cut(s) 197, 480
BseCI ATCGAT 1 cut(s) 15
BseGI GGATG 2 cut(s) 271, 296
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 118, 150
BseNI ACTGG 3 cut(s) 429, 447, 474
BseX3I CGGCCG 1 cut(s) 225
BseXI GCAGC 2 cut(s) 17, 406
BsgI GTGCAG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 1 cut(s) 227
BshVI ATCGAT 1 cut(s) 15
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 3 cut(s) 158, 285, 339
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 404
BslI CCNNNNNNNGG 2 cut(s) 118, 150
BsnI GGCC 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 3 cut(s) 158, 285, 339
Bsp143I GATC 4 cut(s) 238, 271, 298, 468
BspACI CCGC 3 cut(s) 124, 168, 171
BspANI GGCC 1 cut(s) 227
BspDI ATCGAT 1 cut(s) 15
BspPI GGATC 2 cut(s) 279, 306
BspQI GCTCTTC 1 cut(s) 442
BsrBI CCGCTC 2 cut(s) 124, 168
BsrI ACTGG 3 cut(s) 429, 447, 474
BssMI GATC 4 cut(s) 238, 271, 298, 468
Bst2UI CCWGG 2 cut(s) 197, 480
Bst6I CTCTTC 2 cut(s) 51, 442
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 2 cut(s) 271, 296
BstKTI GATC 4 cut(s) 241, 274, 301, 471
BstMBI GATC 4 cut(s) 238, 271, 298, 468
BstMCI CGRYCG 1 cut(s) 228
BstNI CCWGG 2 cut(s) 197, 480
BstSCI CCNGG 2 cut(s) 195, 478
BstV1I GCAGC 2 cut(s) 17, 406
BstX2I RGATCY 3 cut(s) 271, 298, 468
BstYI RGATCY 3 cut(s) 271, 298, 468
BstZI CGGCCG 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 15
BsuRI GGCC 1 cut(s) 227
BsuTUI ATCGAT 1 cut(s) 15
BtsCI GGATG 2 cut(s) 271, 296
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 354
Cfr13I GGNCC 1 cut(s) 249
ClaI ATCGAT 1 cut(s) 15
CsiI ACCWGGT 1 cut(s) 195
Csp6I GTAC 1 cut(s) 214
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 149
DpnI GATC 4 cut(s) 240, 273, 300, 470
DpnII GATC 4 cut(s) 238, 271, 298, 468
DriI GACNNNNNGTC 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 225
EagI CGGCCG 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 51, 442
Eam1105I GACNNNNNGTC 1 cut(s) 241
EarI CTCTTC 2 cut(s) 51, 442
Ecl136II GAGCTC 3 cut(s) 156, 283, 337
EclXI CGGCCG 1 cut(s) 225
Eco24I GRGCYC 3 cut(s) 158, 285, 339
Eco47I GGWCC 1 cut(s) 249
Eco52I CGGCCG 1 cut(s) 225
Eco53kI GAGCTC 3 cut(s) 156, 283, 337
Eco88I CYCGRG 1 cut(s) 332
EcoICRI GAGCTC 3 cut(s) 156, 283, 337
EcoRII CCWGG 2 cut(s) 195, 478
EcoT38I GRGCYC 3 cut(s) 158, 285, 339
FaiI YATR 3 cut(s) 321, 377, 392
FauI CCCGC 1 cut(s) 117
Fnu4HI GCNGC 4 cut(s) 6, 169, 172, 420
FokI GGATG 2 cut(s) 278, 303
FriOI GRGCYC 3 cut(s) 158, 285, 339
Fsp4HI GCNGC 4 cut(s) 6, 169, 172, 420
GluI GCNGC 4 cut(s) 6, 169, 172, 420
GsuI CTGGAG 1 cut(s) 457
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 404
HinfI GANTC 6 cut(s) 17, 71, 83, 221, 306, 428
HpaII CCGG 1 cut(s) 404
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 3 cut(s) 275, 332, 359
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 2 cut(s) 125, 203
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 1 cut(s) 422
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 1 cut(s) 216
Kzo9I GATC 4 cut(s) 238, 271, 298, 468
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 2 cut(s) 121, 153
Lsp1109I GCAGC 2 cut(s) 17, 406
LweI GCATC 1 cut(s) 281
MabI ACCWGGT 1 cut(s) 195
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 2 cut(s) 341, 368
MalI GATC 4 cut(s) 240, 273, 300, 470
MbiI CCGCTC 2 cut(s) 124, 168
MboI GATC 4 cut(s) 238, 271, 298, 468
MfeI CAATTG 1 cut(s) 460
MflI RGATCY 3 cut(s) 271, 298, 468
MhlI GDGCHC 3 cut(s) 158, 285, 339
MluCI AATT 3 cut(s) 137, 327, 460
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 5 cut(s) 52, 121, 226, 240, 262
MroXI GAANNNNTTC 1 cut(s) 327
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 2 cut(s) 197, 480
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 2 cut(s) 197, 480
NdeII GATC 4 cut(s) 238, 271, 298, 468
PciSI GCTCTTC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 5 cut(s) 17, 83, 221, 306, 428
PkrI GCNGC 4 cut(s) 7, 170, 173, 421
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp124BI GAGCTC 3 cut(s) 158, 285, 339
Psp6I CCWGG 2 cut(s) 195, 478
PspGI CCWGG 2 cut(s) 195, 478
PspPI GGNCC 1 cut(s) 249
PsuI RGATCY 3 cut(s) 271, 298, 468
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 3 cut(s) 158, 285, 339
SapI GCTCTTC 1 cut(s) 442
SatI GCNGC 4 cut(s) 6, 169, 172, 420
Sau3AI GATC 4 cut(s) 238, 271, 298, 468
Sau96I GGNCC 1 cut(s) 249
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 197, 480
SduI GDGCHC 3 cut(s) 158, 285, 339
SexAI ACCWGGT 1 cut(s) 195
SfaNI GCATC 1 cut(s) 281
SinI GGWCC 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 3 cut(s) 137, 327, 460
SsiI CCGC 3 cut(s) 124, 168, 171
SstI GAGCTC 3 cut(s) 158, 285, 339
StyD4I CCNGG 2 cut(s) 195, 478
TaiI ACGT 1 cut(s) 219
TaqI TCGA 4 cut(s) 15, 164, 245, 276
TasI AATT 3 cut(s) 137, 327, 460
TauI GCSGC 2 cut(s) 171, 174
TfiI GAWTC 5 cut(s) 17, 83, 221, 306, 428
TscAI CASTG 1 cut(s) 429
TseI GCWGC 2 cut(s) 5, 419
TspDTI ATGAA 2 cut(s) 69, 336
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 429
VpaK11BI GGWCC 1 cut(s) 249
XapI RAATTY 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.