Rh4BG141300

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001712
Physical Location & Seq
Forward (+)
25087657 .. 25088377
721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG141300.1

Sequence Viewer

Length: 558 bp
ATGGCAGCCTATATCGAATCTTGGGTTTCTTCTTCAATCCAAGAAAACCCAAATGAAGAGGAAGCAAATGAGTCATTGGAATCAATCTTCTCTTCTTCTGCGTTCAACCGACAAGTGGAGCGGGAAGAAGGAGAATTGCCCACGCTAAGGAGCTCATCGTCGAGCGGCAGCAGGAAGAAGAAGAAGAAGAACCTGGTCTTGCTTGAAGGGTATGTTGATTCGGCCGATGAGGACGATCTGTCGAGGACCAAGAGCTTGACGGATGAGGATCTCGATGAGCTCAAAGGGTGCTTGGATCTGGGATTCGGGTTCAGCTACGAAGAAATTCCCGAGCTCTGTAACACCTTGCCTGCTCTTGAGCTTTGTTACTCTATGAGCCAGAAGTTCATGGATAAGTCGCCGGAGAGCTCTCCTGCTGCACTGGATTCCTGCTCTTCTGTGTCCAGTCCGATTGCCAATTGGAAGATCTCCAGTCCTGGTGACCATCCAGAGGATGTGAAGGCGAGGCTGAAATACTGGGCACAGGCTGTGGCGTGCACTGTTAGATTGTGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

185

Amino Acids

20.42

Weight (kDa)

4.51

Isoelectric Point (pI)

68.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 86 - 139 4e-25 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 121, 165
AciI CCGC 2 cut(s) 121, 165
AclWI GGATC 2 cut(s) 276, 303
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 1 cut(s) 324
AfiI CCNNNNNNNGG 3 cut(s) 115, 147, 490
AgsI TTSAA 3 cut(s) 36, 106, 206
AhdI GACNNNNNGTC 1 cut(s) 238
AjnI CCWGG 2 cut(s) 192, 475
AluBI AGCT 8 cut(s) 153, 255, 280, 315, 334, 361, 408, 555
AluI AGCT 8 cut(s) 153, 255, 280, 315, 334, 361, 408, 555
Alw21I GWGCWC 5 cut(s) 155, 282, 336, 410, 539
Alw44I GTGCAC 1 cut(s) 535
AlwI GGATC 2 cut(s) 276, 303
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 1 cut(s) 222
ApaLI GTGCAC 1 cut(s) 535
ApeKI GCWGC 4 cut(s) 5, 168, 416, 552
ApoI RAATTY 1 cut(s) 324
Asp700I GAANNNNTTC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 491
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 246
BaeGI GKGCMC 2 cut(s) 523, 539
BanII GRGCYC 4 cut(s) 155, 282, 336, 410
Bbv12I GWGCWC 5 cut(s) 155, 282, 336, 410, 539
BbvI GCAGC 3 cut(s) 17, 180, 403
BccI CCATC 1 cut(s) 492
BciT130I CCWGG 2 cut(s) 194, 477
BfaI CTAG 1 cut(s) 556
BglII AGATCT 1 cut(s) 465
BisI GCNGC 5 cut(s) 6, 166, 169, 417, 553
BlsI GCNGC 5 cut(s) 7, 167, 170, 418, 554
Bme1390I CCNGG 2 cut(s) 194, 477
Bme18I GGWCC 1 cut(s) 246
BmeRI GACNNNNNGTC 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 246
BmrFI CCNGG 2 cut(s) 194, 477
BmrI ACTGGG 1 cut(s) 526
BmuI ACTGGG 1 cut(s) 526
BpmI CTGGAG 1 cut(s) 454
Bpu10I CCTNAGC 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 267
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 147, 490
Bse1I ACTGG 4 cut(s) 426, 444, 471, 521
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 194, 477
BseGI GGATG 3 cut(s) 268, 484, 499
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 115, 147, 490
BseNI ACTGG 4 cut(s) 426, 444, 471, 521
BseSI GKGCMC 2 cut(s) 523, 539
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 3 cut(s) 17, 180, 403
BsgI GTGCAG 1 cut(s) 402
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 224
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 5 cut(s) 155, 282, 336, 410, 539
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 1 cut(s) 401
BslI CCNNNNNNNGG 3 cut(s) 115, 147, 490
BsnI GGCC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 6 cut(s) 155, 282, 336, 410, 523, 539
Bsp143I GATC 4 cut(s) 235, 268, 295, 465
BspACI CCGC 2 cut(s) 121, 165
BspANI GGCC 1 cut(s) 224
BspPI GGATC 2 cut(s) 276, 303
BspQI GCTCTTC 1 cut(s) 439
BsrBI CCGCTC 2 cut(s) 121, 165
BsrI ACTGG 4 cut(s) 426, 444, 471, 521
BssMI GATC 4 cut(s) 235, 268, 295, 465
Bst2UI CCWGG 2 cut(s) 194, 477
Bst4CI ACNGT 1 cut(s) 541
Bst6I CTCTTC 3 cut(s) 51, 97, 439
BstC8I GCNNGC 2 cut(s) 351, 535
BstDEI CTNAG 1 cut(s) 146
BstEII GGTNACC 1 cut(s) 479
BstF5I GGATG 3 cut(s) 268, 484, 499
BstKTI GATC 4 cut(s) 238, 271, 298, 468
BstMBI GATC 4 cut(s) 235, 268, 295, 465
BstMCI CGRYCG 1 cut(s) 225
BstNI CCWGG 2 cut(s) 194, 477
BstPI GGTNACC 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 192, 475
BstSLI GKGCMC 2 cut(s) 523, 539
BstV1I GCAGC 3 cut(s) 17, 180, 403
BstX2I RGATCY 3 cut(s) 268, 295, 465
BstYI RGATCY 3 cut(s) 268, 295, 465
BstZI CGGCCG 1 cut(s) 222
BsuRI GGCC 1 cut(s) 224
BtsCI GGATG 3 cut(s) 268, 484, 499
BtsIMutI CAGTG 2 cut(s) 419, 537
Cac8I GCNNGC 2 cut(s) 351, 535
Cfr13I GGNCC 1 cut(s) 246
CsiI ACCWGGT 1 cut(s) 192
CviAII CATG 1 cut(s) 388
DdeI CTNAG 1 cut(s) 146
DpnI GATC 4 cut(s) 237, 270, 297, 467
DpnII GATC 4 cut(s) 235, 268, 295, 465
DriI GACNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 222
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 3 cut(s) 51, 97, 439
Eam1105I GACNNNNNGTC 1 cut(s) 238
EarI CTCTTC 3 cut(s) 51, 97, 439
Ecl136II GAGCTC 4 cut(s) 153, 280, 334, 408
EclXI CGGCCG 1 cut(s) 222
Eco24I GRGCYC 4 cut(s) 155, 282, 336, 410
Eco47I GGWCC 1 cut(s) 246
Eco52I CGGCCG 1 cut(s) 222
Eco53kI GAGCTC 4 cut(s) 153, 280, 334, 408
Eco88I CYCGRG 1 cut(s) 329
Eco91I GGTNACC 1 cut(s) 479
EcoICRI GAGCTC 4 cut(s) 153, 280, 334, 408
EcoO65I GGTNACC 1 cut(s) 479
EcoRII CCWGG 2 cut(s) 192, 475
EcoT38I GRGCYC 4 cut(s) 155, 282, 336, 410
FaeI CATG 1 cut(s) 391
FaiI YATR 4 cut(s) 12, 213, 374, 389
FatI CATG 1 cut(s) 387
FauI CCCGC 1 cut(s) 114
Fnu4HI GCNGC 5 cut(s) 6, 166, 169, 417, 553
FokI GGATG 3 cut(s) 275, 471, 506
FriOI GRGCYC 4 cut(s) 155, 282, 336, 410
Fsp4HI GCNGC 5 cut(s) 6, 166, 169, 417, 553
FspBI CTAG 1 cut(s) 556
GluI GCNGC 5 cut(s) 6, 166, 169, 417, 553
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 1 cut(s) 224
HapII CCGG 1 cut(s) 401
Hin1II CATG 1 cut(s) 391
HinfI GANTC 6 cut(s) 17, 71, 80, 218, 303, 425
HpaII CCGG 1 cut(s) 401
HphI GGTGA 1 cut(s) 491
Hpy166II GTNNAC 1 cut(s) 537
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 4 cut(s) 272, 329, 356, 488
Hpy8I GTNNAC 1 cut(s) 537
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 3 cut(s) 122, 200, 493
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4V TGCA 3 cut(s) 419, 537, 552
HpyF3I CTNAG 1 cut(s) 146
Hsp92II CATG 1 cut(s) 391
Kzo9I GATC 4 cut(s) 235, 268, 295, 465
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 2 cut(s) 118, 150
Lsp1109I GCAGC 3 cut(s) 17, 180, 403
MabI ACCWGGT 1 cut(s) 192
MaeI CTAG 1 cut(s) 556
MaeIII GTNAC 3 cut(s) 338, 365, 479
MalI GATC 4 cut(s) 237, 270, 297, 467
MbiI CCGCTC 2 cut(s) 121, 165
MboI GATC 4 cut(s) 235, 268, 295, 465
MfeI CAATTG 1 cut(s) 457
MflI RGATCY 3 cut(s) 268, 295, 465
MhlI GDGCHC 6 cut(s) 155, 282, 336, 410, 523, 539
MluCI AATT 3 cut(s) 134, 324, 457
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 6 cut(s) 52, 223, 237, 259, 484, 498
MroXI GAANNNNTTC 1 cut(s) 324
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 2 cut(s) 194, 477
MunI CAATTG 1 cut(s) 457
MvaI CCWGG 2 cut(s) 194, 477
NdeII GATC 4 cut(s) 235, 268, 295, 465
NlaIII CATG 1 cut(s) 391
NmuCI GTSAC 1 cut(s) 479
PciSI GCTCTTC 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 5 cut(s) 17, 80, 218, 303, 425
PkrI GCNGC 5 cut(s) 7, 167, 170, 418, 554
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp124BI GAGCTC 4 cut(s) 155, 282, 336, 410
Psp6I CCWGG 2 cut(s) 192, 475
PspEI GGTNACC 1 cut(s) 479
PspGI CCWGG 2 cut(s) 192, 475
PspPI GGNCC 1 cut(s) 246
PsuI RGATCY 3 cut(s) 268, 295, 465
SacI GAGCTC 4 cut(s) 155, 282, 336, 410
SapI GCTCTTC 1 cut(s) 439
SatI GCNGC 5 cut(s) 6, 166, 169, 417, 553
Sau3AI GATC 4 cut(s) 235, 268, 295, 465
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 194, 477
SduI GDGCHC 6 cut(s) 155, 282, 336, 410, 523, 539
SexAI ACCWGGT 1 cut(s) 192
SinI GGWCC 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 3 cut(s) 134, 324, 457
SsiI CCGC 2 cut(s) 121, 165
SspMI CTAG 1 cut(s) 556
SstI GAGCTC 4 cut(s) 155, 282, 336, 410
StyD4I CCNGG 2 cut(s) 192, 475
TaaI ACNGT 1 cut(s) 541
TaqI TCGA 4 cut(s) 15, 161, 242, 273
TasI AATT 3 cut(s) 134, 324, 457
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 5 cut(s) 17, 80, 218, 303, 425
TscAI CASTG 2 cut(s) 426, 544
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 4 cut(s) 5, 168, 416, 552
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 2 cut(s) 69, 376
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 2 cut(s) 426, 544
VneI GTGCAC 1 cut(s) 535
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 1 cut(s) 324
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.