FvH4_4g11250

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001712
Physical Location & Seq
Forward (+)
15041581 .. 15043410
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11250.t1

Sequence Viewer

Length: 447 bp
ATGGACGCCTTCACCGAATCTCTAGAATCAATCTACTCTTCTTCTGCCTTCAACAGACAAGTGGAGCGAGAAGAAGCAGAATTACCCAAGCTGAGAAGCTTGTCTTCGAGGGAGAAGAAGAAGAATCAGGTGTTGCTTGAAGGCTATGTGGATTCTGACGATCATCTGTCGAGGACCAAGAGCTTGACGGATGAGGATCTCGATGAGCTCAAAGGCTGTTTGGATCTCGGATTTGGCTTCAGCTATCAAGAGATTCCCGAGCTCTGTAACACCTTGCCGGCTCTTGAGCTCTGCTACTCTATGAGCCACAAGTTCATGCTCCATTCCTGCTCTTCTCCGATCGCCAATTGGAAGATCTCCAGTCCTGGTGACCATCCAGAGGATGTGAAGGCGAGGCTCAAATACTGGGCACAGGCTGTTGCCTGCACACTTAAATTGTGCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

149

Amino Acids

16.81

Weight (kDa)

5.23

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 62 - 107 2.9e-23 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 204, 231
AcuI CTGAAG 1 cut(s) 223
AcyI GRCGYC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 2 cut(s) 52, 140
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 7 cut(s) 91, 99, 183, 208, 243, 262, 289
AluI AGCT 7 cut(s) 91, 99, 183, 208, 243, 262, 289
Alw21I GWGCWC 3 cut(s) 210, 264, 291
AlwI GGATC 2 cut(s) 204, 231
Ama87I CYCGRG 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 174
AsuHPI GGTGA 2 cut(s) 4, 380
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 412
BanII GRGCYC 3 cut(s) 210, 264, 291
BarI GAAGNNNNNNTAC 2 cut(s) 66, 98
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 3 cut(s) 210, 264, 291
BccI CCATC 1 cut(s) 381
BciT130I CCWGG 1 cut(s) 366
BfaI CTAG 1 cut(s) 23
BglII AGATCT 1 cut(s) 354
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 1 cut(s) 174
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 366
BmrI ACTGGG 1 cut(s) 415
BmuI ACTGGG 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 343
BpuEI CTTGAG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 6
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 360, 410
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 1 cut(s) 366
BseGI GGATG 3 cut(s) 196, 373, 388
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 2 cut(s) 360, 410
BseSI GKGCMC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 409
Bsh1285I CGRYCG 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 3 cut(s) 210, 264, 291
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 4 cut(s) 210, 264, 291, 412
Bsp143I GATC 5 cut(s) 160, 196, 223, 339, 354
BspCNI CTCAG 1 cut(s) 84
BspPI GGATC 2 cut(s) 204, 231
BspQI GCTCTTC 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 2 cut(s) 360, 410
BssAI RCCGGY 1 cut(s) 277
BssMI GATC 5 cut(s) 160, 196, 223, 339, 354
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 366
Bst6I CTCTTC 2 cut(s) 43, 337
BstACI GRCGYC 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 279, 424
BstDEI CTNAG 1 cut(s) 92
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 3 cut(s) 196, 373, 388
BstKTI GATC 5 cut(s) 163, 199, 226, 342, 357
BstMBI GATC 5 cut(s) 160, 196, 223, 339, 354
BstMCI CGRYCG 1 cut(s) 342
BstNI CCWGG 1 cut(s) 366
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 364
BstSLI GKGCMC 1 cut(s) 412
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 3 cut(s) 196, 223, 354
BstYI RGATCY 3 cut(s) 196, 223, 354
BtsCI GGATG 3 cut(s) 196, 373, 388
Cac8I GCNNGC 2 cut(s) 279, 424
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 174
CseI GACGC 1 cut(s) 14
CviAII CATG 1 cut(s) 316
DdeI CTNAG 1 cut(s) 92
DpnI GATC 5 cut(s) 162, 198, 225, 341, 356
DpnII GATC 5 cut(s) 160, 196, 223, 339, 354
Eam1104I CTCTTC 2 cut(s) 43, 337
EarI CTCTTC 2 cut(s) 43, 337
Ecl136II GAGCTC 3 cut(s) 208, 262, 289
Eco24I GRGCYC 3 cut(s) 210, 264, 291
Eco47I GGWCC 1 cut(s) 174
Eco53kI GAGCTC 3 cut(s) 208, 262, 289
Eco57I CTGAAG 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 368
EcoICRI GAGCTC 3 cut(s) 208, 262, 289
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 364
EcoT38I GRGCYC 3 cut(s) 210, 264, 291
FaeI CATG 1 cut(s) 319
FaiI YATR 3 cut(s) 147, 302, 317
FalI AAGNNNNNCTT 2 cut(s) 88, 120
FatI CATG 1 cut(s) 315
FokI GGATG 3 cut(s) 203, 360, 395
FriOI GRGCYC 3 cut(s) 210, 264, 291
FspBI CTAG 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 343
HapII CCGG 1 cut(s) 278
HgaI GACGC 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 97
HinfI GANTC 5 cut(s) 17, 26, 124, 152, 253
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 4, 380
Hpy188I TCNGA 3 cut(s) 157, 230, 339
Hpy188III TCNNGA 6 cut(s) 23, 200, 248, 257, 284, 377
HpyAV CCTTC 4 cut(s) 19, 58, 134, 382
HpyCH4V TGCA 2 cut(s) 426, 441
HpyF3I CTNAG 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 1 cut(s) 319
KroI GCCGGC 1 cut(s) 277
KroNI GCCGGC 1 cut(s) 279
Kzo9I GATC 5 cut(s) 160, 196, 223, 339, 354
LguI GCTCTTC 1 cut(s) 337
LmnI GCTCC 2 cut(s) 64, 324
MaeI CTAG 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 266, 368
MalI GATC 5 cut(s) 162, 198, 225, 341, 356
MboI GATC 5 cut(s) 160, 196, 223, 339, 354
MboII GAAGA 9 cut(s) 30, 33, 83, 96, 127, 130, 133, 324, 364
MfeI CAATTG 1 cut(s) 346
MflI RGATCY 3 cut(s) 196, 223, 354
MhlI GDGCHC 4 cut(s) 210, 264, 291, 412
MluCI AATT 3 cut(s) 80, 346, 434
MnlI CCTC 5 cut(s) 102, 165, 187, 373, 387
MroNI GCCGGC 1 cut(s) 277
MseI TTAA 1 cut(s) 432
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 366
MunI CAATTG 1 cut(s) 346
MvaI CCWGG 1 cut(s) 366
NaeI GCCGGC 1 cut(s) 279
NdeII GATC 5 cut(s) 160, 196, 223, 339, 354
NgoMIV GCCGGC 1 cut(s) 277
NlaIII CATG 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 368
PciSI GCTCTTC 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 279
PfeI GAWTC 5 cut(s) 17, 26, 124, 152, 253
Ple19I CGATCG 1 cut(s) 342
Psp124BI GAGCTC 3 cut(s) 210, 264, 291
Psp6I CCWGG 1 cut(s) 364
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 1 cut(s) 364
PspPI GGNCC 1 cut(s) 174
PsuI RGATCY 3 cut(s) 196, 223, 354
PvuI CGATCG 1 cut(s) 342
SacI GAGCTC 3 cut(s) 210, 264, 291
SapI GCTCTTC 1 cut(s) 337
SaqAI TTAA 1 cut(s) 432
Sau3AI GATC 5 cut(s) 160, 196, 223, 339, 354
Sau96I GGNCC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 4 cut(s) 210, 264, 291, 412
SetI ASST 9 cut(s) 93, 101, 132, 185, 210, 245, 264, 275, 291
SinI GGWCC 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 3 cut(s) 80, 346, 434
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 3 cut(s) 210, 264, 291
StyD4I CCNGG 1 cut(s) 364
TaqI TCGA 3 cut(s) 107, 170, 201
TasI AATT 3 cut(s) 80, 346, 434
TfiI GAWTC 5 cut(s) 17, 26, 124, 152, 253
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseFI GTSAC 1 cut(s) 368
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 1 cut(s) 304
TspGWI ACGGA 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 174
XbaI TCTAGA 1 cut(s) 22
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.