AT3G04300

function DUF861, cupin-3 (InterPro IPR008579), RmlC-like jelly roll fold (InterPro IPR014710)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003853
Physical Location & Seq
Forward (+)
1140129 .. 1141060
932 bp
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UTR
Exon/CDS
Intron
AT3G04300.1

Sequence Viewer

Length: 291 bp
ATGAATATTGTAATCGAAAACAACCCTTCGAGCAGAAGGTTATCTGACCTTGGAGTCATGTCATGGCCTAAATGGTCTTGTCAGCCGGGGAAATATGCATTGGTATTTGAAGAAAGAGAGACATGCTATTTGGTGAAGGGAAAGGTGAAGGTGTATCCAAAAGGGTCATCTGAGTTTGTAGAGTTTGGTGCAGGAGACCTTGTGACCATCCCCAAGGGACTTAGCTGCACTTGGGATGTATCACTTTTCATAGACAAACACTACAAGTTCGATCCTCCTACTTCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

96

Amino Acids

10.85

Weight (kDa)

7.68

Isoelectric Point (pI)

57.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 15 - 88 1.8e-29 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 53
AclWI GGATC 1 cut(s) 266
AgsI TTSAA 1 cut(s) 110
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AluBI AGCT 1 cut(s) 225
AluI AGCT 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 113, 189
AlwI GGATC 1 cut(s) 266
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 1 cut(s) 225
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 2 cut(s) 145, 157
BbvI GCAGC 1 cut(s) 212
BccI CCATC 1 cut(s) 215
BciVI GTATCC 1 cut(s) 165
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 113, 189
BfuI GTATCC 1 cut(s) 165
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 49, 86, 213
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
BseDI CCNNGG 3 cut(s) 49, 86, 213
BseGI GGATG 2 cut(s) 207, 241
BseMII CTCAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 212
BsgI GTGCAG 2 cut(s) 210, 211
BshFI GGCC 1 cut(s) 67
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 1 cut(s) 231
BsmAI GTCTC 2 cut(s) 113, 189
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 271
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 189
BssECI CCNNGG 3 cut(s) 49, 86, 213
BssMI GATC 1 cut(s) 271
BssT1I CCWWGG 2 cut(s) 49, 213
BstDEI CTNAG 2 cut(s) 171, 221
BstF5I GGATG 2 cut(s) 207, 241
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 2 cut(s) 113, 189
BstMBI GATC 1 cut(s) 271
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 212
BsuI GTATCC 1 cut(s) 165
BsuRI GGCC 1 cut(s) 67
BtsCI GGATG 2 cut(s) 207, 241
CviAII CATG 3 cut(s) 58, 63, 123
CviJI RGCY 3 cut(s) 67, 85, 225
CviKI_1 RGCY 3 cut(s) 67, 85, 225
DdeI CTNAG 2 cut(s) 171, 221
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 49, 213
Eco31I GGTCTC 1 cut(s) 189
EcoT14I CCWWGG 2 cut(s) 49, 213
EcoT22I ATGCAT 1 cut(s) 100
ErhI CCWWGG 2 cut(s) 49, 213
FaeI CATG 3 cut(s) 61, 66, 126
FaiI YATR 6 cut(s) 59, 64, 96, 124, 251, 289
FaqI GGGAC 1 cut(s) 231
FatI CATG 3 cut(s) 57, 62, 122
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 2 cut(s) 194, 248
Fsp4HI GCNGC 1 cut(s) 226
GluI GCNGC 1 cut(s) 226
HaeIII GGCC 1 cut(s) 67
HapII CCGG 1 cut(s) 86
Hin1II CATG 3 cut(s) 61, 66, 126
HinfI GANTC 1 cut(s) 54
HpaII CCGG 1 cut(s) 86
HphI GGTGA 2 cut(s) 145, 157
Hpy188I TCNGA 2 cut(s) 46, 172
HpyAV CCTTC 4 cut(s) 30, 36, 130, 142
HpyCH4V TGCA 3 cut(s) 98, 191, 228
HpyF3I CTNAG 2 cut(s) 171, 221
Hsp92II CATG 3 cut(s) 61, 66, 126
Kzo9I GATC 1 cut(s) 271
LpnPI CCDG 2 cut(s) 99, 177
Lsp1109I GCAGC 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 202
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 1 cut(s) 122
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 1 cut(s) 285
Mph1103I ATGCAT 1 cut(s) 100
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 87
NciI CCSGG 1 cut(s) 87
NdeII GATC 1 cut(s) 271
NlaIII CATG 3 cut(s) 61, 66, 126
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 100
NspI RCATGY 1 cut(s) 126
PkrI GCNGC 1 cut(s) 227
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 1 cut(s) 271
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 87
SetI ASST 6 cut(s) 41, 51, 147, 153, 201, 227
SspI AATATT 1 cut(s) 7
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 2 cut(s) 49, 213
TaqI TCGA 3 cut(s) 15, 29, 270
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 225
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 17, 238
XceI RCATGY 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.