pycom04g20630
ERF Family

superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0003853
Physical Location & Seq
Forward (+)
22100112 .. 22100826
715 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20630.1

Sequence Viewer

Length: 345 bp
ATGGCAGCAACAACATCATCAAGTACTTCTGCAGAAGCACTTCCCAATCTAAGAATCGTAGTAGAAAGCAACCCTTCCAATTCACGATTATCTGAGTTGGGTATTAACTCATGGCCCAAATGGGGATGTCCACCGGGGAAGTACACATTGAAGTTTGATGCAGCGGAGACATGTTATCTGTTGAAAGGCAAGGTGAAGGTTTACCCTAGAAAAGGCTCGTTGTCATCTGATGAGGAGTTTGTAGAGTTTGGTGCTGGAGACCTTGTGACCATTCCCAAGGGACTCAGTTGCACATGGGACGTCTCAGTTGCTATTGATAAACACTACAAATTCGATTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

115

Amino Acids

12.38

Weight (kDa)

6.56

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 31 - 109 1.4e-25 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 303
AciI CCGC 1 cut(s) 164
AcsI RAATTY 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 2 cut(s) 25, 143
AfiI CCNNNNNNNGG 2 cut(s) 122, 212
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 2 cut(s) 151, 184
Alw26I GTCTC 3 cut(s) 161, 252, 307
AoxI GGCC 1 cut(s) 113
ApeKI GCWGC 2 cut(s) 5, 161
ApoI RAATTY 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 114
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 1 cut(s) 205
BbvI GCAGC 2 cut(s) 17, 173
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 161, 252, 307
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 1 cut(s) 30
BisI GCNGC 2 cut(s) 6, 162
BlsI GCNGC 2 cut(s) 7, 163
BmcAI AGTACT 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 135
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 134, 276
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 212
BseDI CCNNGG 2 cut(s) 134, 276
BseGI GGATG 1 cut(s) 131
BseLI CCNNNNNNNGG 2 cut(s) 122, 212
BseMII CTCAG 3 cut(s) 84, 298, 318
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 17, 173
BshFI GGCC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 2 cut(s) 294, 311
BslI CCNNNNNNNGG 2 cut(s) 122, 212
BsmAI GTCTC 3 cut(s) 161, 252, 307
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 2 cut(s) 294, 311
BsnI GGCC 1 cut(s) 115
Bso31I GGTCTC 1 cut(s) 252
BspACI CCGC 1 cut(s) 164
BspANI GGCC 1 cut(s) 115
BspCNI CTCAG 3 cut(s) 85, 297, 317
BspHI TCATGA 1 cut(s) 341
BspMAI CTGCAG 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 252
BssECI CCNNGG 2 cut(s) 134, 276
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 300
BstDEI CTNAG 4 cut(s) 50, 93, 284, 304
BstENI CCTNNNNNAGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 131
BstMAI GTCTC 3 cut(s) 161, 252, 307
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 17, 173
BsuRI GGCC 1 cut(s) 115
BtsCI GGATG 1 cut(s) 131
CciI TCATGA 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 24, 142
CviAII CATG 4 cut(s) 111, 171, 294, 342
CviJI RGCY 2 cut(s) 115, 216
CviKI_1 RGCY 2 cut(s) 115, 216
CviQI GTAC 2 cut(s) 24, 142
DdeI CTNAG 4 cut(s) 50, 93, 284, 304
Eco130I CCWWGG 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 252
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 4 cut(s) 114, 174, 297, 345
FaiI YATR 4 cut(s) 112, 172, 295, 343
FalI AAGNNNNNCTT 2 cut(s) 58, 90
FaqI GGGAC 2 cut(s) 294, 311
FatI CATG 4 cut(s) 110, 170, 293, 341
Fnu4HI GCNGC 2 cut(s) 6, 162
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 6, 162
FspBI CTAG 1 cut(s) 207
GluI GCNGC 2 cut(s) 6, 162
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 1 cut(s) 115
HapII CCGG 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 4 cut(s) 114, 174, 297, 345
HinfI GANTC 3 cut(s) 54, 282, 335
HpaII CCGG 1 cut(s) 134
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 3 cut(s) 131, 144, 202
Hpy188I TCNGA 2 cut(s) 94, 229
Hpy188III TCNNGA 2 cut(s) 84, 342
Hpy8I GTNNAC 3 cut(s) 131, 144, 202
HpyAV CCTTC 2 cut(s) 84, 190
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 3 cut(s) 32, 161, 291
HpyF3I CTNAG 4 cut(s) 50, 93, 284, 304
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 4 cut(s) 114, 174, 297, 345
LpnPI CCDG 2 cut(s) 147, 240
Lsp1109I GCAGC 2 cut(s) 17, 173
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 207
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 265
MboII GAAGA 1 cut(s) 330
MluCI AATT 2 cut(s) 79, 329
MlyI GAGTC 1 cut(s) 276
MnlI CCTC 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 135
NciI CCSGG 1 cut(s) 135
NlaIII CATG 4 cut(s) 114, 174, 297, 345
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 174
PagI TCATGA 1 cut(s) 341
PciI ACATGT 1 cut(s) 170
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 2 cut(s) 54, 335
PkrI GCNGC 2 cut(s) 7, 163
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
PscI ACATGT 1 cut(s) 170
PspPI GGNCC 1 cut(s) 114
PstI CTGCAG 1 cut(s) 34
RsaI GTAC 2 cut(s) 25, 143
RsaNI GTAC 2 cut(s) 24, 142
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 6, 162
Sau96I GGNCC 1 cut(s) 114
ScaI AGTACT 1 cut(s) 25
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 1 cut(s) 135
SetI ASST 4 cut(s) 195, 201, 264, 303
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 30
Sse9I AATT 2 cut(s) 79, 329
SsiI CCGC 1 cut(s) 164
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 276
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 333
TasI AATT 2 cut(s) 79, 329
TatI WGTACW 2 cut(s) 23, 141
TfiI GAWTC 2 cut(s) 54, 335
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 5, 161
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 330
XagI CCTNNNNNAGG 1 cut(s) 210
XapI RAATTY 1 cut(s) 329
XceI RCATGY 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 1 cut(s) 207
ZraI GACGTC 1 cut(s) 301
ZrmI AGTACT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.