pycom10g15000

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003853
Physical Location & Seq
Reverse (-)
18347870 .. 18348514
645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g15000.1

Sequence Viewer

Length: 348 bp
ATGGCTGCAGAACCCTCCTCTTCAGCAAGTTCCTCATCATCTTCAGACCTAAGAATCATAGTTGAAAGAAACCCTTCTGAATCTCGCTTATCCCAACTGGACATCAAGAGCTGGCCCAAATGGGGTTGCTCGCCGGGGAAGTACCAGCTGAAGTTTGATGCAGAGGAGACGTGTTATTTGTTGAAGGGAAGAGTGAAGGCATATTATCCAAAAGGGTCGTCGGAATATGTCGAGTTTGGAGCTGGAGATCTTGTCACCATCCCAAGGGGACTCACCTGCACTTGGGATGTTTCTCTTGCTGTTGATAAACACTACAAATTCGAATCTTCTTCATCTTCTTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

116

Amino Acids

12.64

Weight (kDa)

6.57

Isoelectric Point (pI)

55.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 31 - 105 2.9e-24 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 284
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 3 cut(s) 6, 27, 170
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 122, 264, 282
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 2 cut(s) 65, 184
AjiI CACGTC 1 cut(s) 171
AluBI AGCT 3 cut(s) 111, 148, 242
AluI AGCT 3 cut(s) 111, 148, 242
Alw26I GTCTC 1 cut(s) 161
AoxI GGCC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 317
ArsI GACNNNNNNTTYG 4 cut(s) 160, 192, 203, 235
Asp700I GAANNNNTTC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 114
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 247, 265
AsuII TTCGAA 1 cut(s) 321
BccI CCATC 1 cut(s) 266
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 284
BglII AGATCT 1 cut(s) 247
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 264
Bpu14I TTCGAA 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 134, 263
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 264, 282
Bse1I ACTGG 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 134, 263
BseGI GGATG 2 cut(s) 258, 292
BseLI CCNNNNNNNGG 3 cut(s) 122, 264, 282
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 2 cut(s) 7, 179
BsgI GTGCAG 1 cut(s) 262
BshFI GGCC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 3 cut(s) 122, 264, 282
BsmAI GTCTC 1 cut(s) 161
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 1 cut(s) 115
Bsp119I TTCGAA 1 cut(s) 321
Bsp143I GATC 1 cut(s) 247
BspANI GGCC 1 cut(s) 115
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 284
BspT104I TTCGAA 1 cut(s) 321
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 134, 263
BssMI GATC 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 263
Bst6I CTCTTC 2 cut(s) 25, 184
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 113, 131
BstDEI CTNAG 1 cut(s) 50
BstF5I GGATG 2 cut(s) 258, 292
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 1 cut(s) 115
BtrI CACGTC 1 cut(s) 171
BtsCI GGATG 2 cut(s) 258, 292
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 113, 131
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 142
CviJI RGCY 5 cut(s) 5, 111, 115, 148, 242
CviKI_1 RGCY 5 cut(s) 5, 111, 115, 148, 242
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 1 cut(s) 50
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 25, 184
EarI CTCTTC 2 cut(s) 25, 184
Eco130I CCWWGG 1 cut(s) 263
Eco57I CTGAAG 3 cut(s) 6, 27, 170
EcoT14I CCWWGG 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 263
Esp3I CGTCTC 1 cut(s) 161
FaiI YATR 3 cut(s) 59, 202, 228
FalI AAGNNNNNCTT 2 cut(s) 58, 90
FaqI GGGAC 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 245, 299
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 1 cut(s) 115
HapII CCGG 1 cut(s) 134
HinfI GANTC 4 cut(s) 54, 80, 270, 323
HpaII CCGG 1 cut(s) 134
HphI GGTGA 2 cut(s) 247, 265
Hpy188I TCNGA 3 cut(s) 46, 79, 223
Hpy188III TCNNGA 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 3 cut(s) 84, 178, 190
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 3 cut(s) 8, 161, 279
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 1 cut(s) 170
Kzo9I GATC 1 cut(s) 247
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 6 cut(s) 83, 97, 147, 158, 228, 289
LweI GCATC 1 cut(s) 148
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 253
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 8 cut(s) 12, 33, 201, 318, 321, 327, 330, 333
MflI RGATCY 1 cut(s) 247
MluCI AATT 1 cut(s) 317
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 4 cut(s) 25, 28, 43, 157
MroXI GAANNNNTTC 1 cut(s) 73
MseI TTAA 1 cut(s) 346
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 135
NciI CCSGG 1 cut(s) 135
NdeII GATC 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 253
NspV TTCGAA 1 cut(s) 321
PaqCI CACCTGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 3 cut(s) 54, 80, 323
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 114
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 346
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 1 cut(s) 135
SetI ASST 6 cut(s) 51, 113, 150, 173, 244, 278
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 321
Sse9I AATT 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 263
TaiI ACGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 231, 321
TasI AATT 1 cut(s) 317
TfiI GAWTC 3 cut(s) 54, 80, 323
Tru1I TTAA 1 cut(s) 346
Tru9I TTAA 1 cut(s) 346
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 321
XapI RAATTY 1 cut(s) 317
XmnI GAANNNNTTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.