pycom12g23050
ERF Family

superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003853
Physical Location & Seq
Forward (+)
23755810 .. 23756556
747 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23050.1

Sequence Viewer

Length: 354 bp
ATGGCAGCTACAGCATCATCAAATGCTTCTGCAGAAGCACTTTCCAATCTAAGAATCGTAGTAGAAAGCAATCCCTCTGATTCACGATTATCTGAGTTGGGTATTAACTCATGGCCCAAATGGGGGTGTCCGCCTGGAAAGTACACATTGAAGTTTGACGCAGCGGAAACATGTTATCTGGTGAAAGGCAAGGTGAAGGTTTACCCTAAAAAAGGCTCATCATCATCATCGTCTTCTGATGAGGAGTTTGTAAATTTTGGTGCTGGGGACCTTGTGACCATTCCCAAGGGACTCAGTTGCATATGGGACGTCTCAGTTGCTATCGATAAACACTACAAATTCGATTCTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

118

Amino Acids

12.53

Weight (kDa)

6.55

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 31 - 112 3.1e-24 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
AciI CCGC 2 cut(s) 131, 164
AcsI RAATTY 2 cut(s) 253, 338
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 122, 123, 212
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 316
AoxI GGCC 1 cut(s) 113
ApeKI GCWGC 2 cut(s) 5, 161
ApoI RAATTY 2 cut(s) 253, 338
AspS9I GGNCC 2 cut(s) 114, 268
AsuHPI GGTGA 2 cut(s) 193, 205
AvaII GGWCC 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 17, 173
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 316
BfmI CTRYAG 2 cut(s) 9, 30
BisI GCNGC 2 cut(s) 6, 162
BlsI GCNGC 2 cut(s) 7, 163
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 114, 268
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 225
Bsa29I ATCGAT 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 1 cut(s) 285
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 123, 212
BseBI CCWGG 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 285
BseLI CCNNNNNNNGG 3 cut(s) 122, 123, 212
BseMII CTCAG 3 cut(s) 84, 307, 327
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 17, 173
BseYI CCCAGC 1 cut(s) 263
BshFI GGCC 1 cut(s) 115
BshVI ATCGAT 1 cut(s) 324
BslFI GGGAC 3 cut(s) 281, 303, 320
BslI CCNNNNNNNGG 3 cut(s) 122, 123, 212
BsmAI GTCTC 1 cut(s) 316
BsmBI CGTCTC 1 cut(s) 316
BsmFI GGGAC 3 cut(s) 281, 303, 320
BsnI GGCC 1 cut(s) 115
BspACI CCGC 2 cut(s) 131, 164
BspANI GGCC 1 cut(s) 115
BspCNI CTCAG 3 cut(s) 85, 306, 326
BspDI ATCGAT 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 269
BspMAI CTGCAG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 285
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 309
BstDEI CTNAG 4 cut(s) 50, 93, 293, 313
BstENI CCTNNNNNAGG 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 2 cut(s) 9, 30
BstV1I GCAGC 2 cut(s) 17, 173
BstV2I GAAGAC 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 1 cut(s) 115
BsuTUI ATCGAT 1 cut(s) 324
Cfr13I GGNCC 2 cut(s) 114, 268
ClaI ATCGAT 1 cut(s) 324
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 142
CviAII CATG 2 cut(s) 111, 171
CviJI RGCY 3 cut(s) 8, 115, 216
CviKI_1 RGCY 3 cut(s) 8, 115, 216
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 4 cut(s) 50, 93, 293, 313
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoO109I RGGNCCY 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 285
Esp3I CGTCTC 1 cut(s) 316
FaeI CATG 2 cut(s) 114, 174
FaiI YATR 4 cut(s) 112, 172, 302, 304
FaqI GGGAC 3 cut(s) 281, 303, 320
FatI CATG 2 cut(s) 110, 170
FauNDI CATATG 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 6, 162
Fsp4HI GCNGC 2 cut(s) 6, 162
GluI GCNGC 2 cut(s) 6, 162
GsaI CCCAGC 1 cut(s) 267
HaeIII GGCC 1 cut(s) 115
HgaI GACGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 2 cut(s) 114, 174
HinfI GANTC 4 cut(s) 54, 80, 291, 344
HphI GGTGA 2 cut(s) 193, 205
Hpy166II GTNNAC 2 cut(s) 144, 202
Hpy188I TCNGA 3 cut(s) 79, 94, 238
Hpy188III TCNNGA 2 cut(s) 84, 351
Hpy8I GTNNAC 2 cut(s) 144, 202
HpyAV CCTTC 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 2 cut(s) 32, 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 4 cut(s) 50, 93, 293, 313
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 2 cut(s) 114, 174
LpnPI CCDG 4 cut(s) 120, 147, 164, 249
Lsp1109I GCAGC 2 cut(s) 17, 173
LweI GCATC 1 cut(s) 23
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 274
MboII GAAGA 2 cut(s) 225, 339
MluCI AATT 2 cut(s) 253, 338
MlyI GAGTC 1 cut(s) 285
MnlI CCTC 2 cut(s) 85, 235
MseI TTAA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeI CATATG 1 cut(s) 302
NlaIII CATG 2 cut(s) 114, 174
NlaIV GGNNCC 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 274
NspI RCATGY 1 cut(s) 174
PciI ACATGT 1 cut(s) 170
PfeI GAWTC 3 cut(s) 54, 80, 344
PkrI GCNGC 2 cut(s) 7, 163
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
PpuMI RGGWCCY 1 cut(s) 268
PscI ACATGT 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 133
PspFI CCCAGC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 269
PspPI GGNCC 2 cut(s) 114, 268
PspPPI RGGWCCY 1 cut(s) 268
PstI CTGCAG 1 cut(s) 34
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 6, 162
Sau96I GGNCC 2 cut(s) 114, 268
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 1 cut(s) 135
SetI ASST 5 cut(s) 10, 195, 201, 273, 312
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 2 cut(s) 9, 30
SinI GGWCC 1 cut(s) 268
Sse9I AATT 2 cut(s) 253, 338
SsiI CCGC 2 cut(s) 131, 164
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 285
TaiI ACGT 1 cut(s) 312
TaqI TCGA 2 cut(s) 324, 342
TasI AATT 2 cut(s) 253, 338
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 3 cut(s) 54, 80, 344
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 5, 161
Tsp45I GTSAC 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 268
XagI CCTNNNNNAGG 1 cut(s) 210
XapI RAATTY 2 cut(s) 253, 338
XceI RCATGY 1 cut(s) 174
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.