MD05G1185700.v1.1

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003853
Physical Location & Seq
Reverse (-)
31446518 .. 31447565
1048 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1185700.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGCTGCAGAACCCTGCTCCTCATCGTCATCAAGTTCCTCATCATCTTCAAACCTAAGAATCACAGTTGAAAGAAACCCTTCTGAATCTCGCTTATCCCAACTGGACATCAAGTGCTGGCCCAAATGGGGTTGCTCGCCGGGGAAGTACCAGCTGAAGTTTGATGCGGAGGAGACGTGTTATTTGTTGAAGGGAAGAGTGAAGGCGTATTATCCAAAAGGGTCGTCGGAATATGTCGAGTTCGGAGCTGGAGATCTTGTCACCATCCCAAAGGGACTCACCTGCACTTGGGATGTTTCTCTTGCTGTTGATAAACACTACAAATTCGAATCTTCTTCTTCTTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

117

Amino Acids

12.74

Weight (kDa)

7.58

Isoelectric Point (pI)

59.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 33 - 107 5.5e-24 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 290
Acc36I ACCTGC 1 cut(s) 290
AciI CCGC 1 cut(s) 167
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 128, 288
AflIII ACRYGT 1 cut(s) 176
AgsI TTSAA 3 cut(s) 51, 71, 190
AjiI CACGTC 1 cut(s) 177
AluBI AGCT 2 cut(s) 154, 248
AluI AGCT 2 cut(s) 154, 248
Alw26I GTCTC 1 cut(s) 167
AoxI GGCC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 323
ArsI GACNNNNNNTTYG 4 cut(s) 166, 198, 209, 241
Asp700I GAANNNNTTC 1 cut(s) 79
AspS9I GGNCC 1 cut(s) 120
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 2 cut(s) 253, 271
AsuII TTCGAA 1 cut(s) 327
BccI CCATC 1 cut(s) 272
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 290
BglII AGATCT 1 cut(s) 253
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 141
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 270
Bpu14I TTCGAA 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 140
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 288
Bse1I ACTGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 2 cut(s) 264, 298
BseLI CCNNNNNNNGG 2 cut(s) 128, 288
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 2 cut(s) 10, 185
BsgI GTGCAG 1 cut(s) 268
BshFI GGCC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 128, 288
BsmAI GTCTC 1 cut(s) 167
BsmBI CGTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 288
BsnI GGCC 1 cut(s) 121
Bsp119I TTCGAA 1 cut(s) 327
Bsp143I GATC 1 cut(s) 253
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 121
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 290
BspT104I TTCGAA 1 cut(s) 327
BsrI ACTGG 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 190
BstBI TTCGAA 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 119, 137
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 2 cut(s) 264, 298
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 139
BstSFI CTRYAG 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 121
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 2 cut(s) 264, 298
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 2 cut(s) 119, 137
Cfr13I GGNCC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 148
CviJI RGCY 4 cut(s) 5, 121, 154, 248
CviKI_1 RGCY 4 cut(s) 5, 121, 154, 248
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 56
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 176
Esp3I CGTCTC 1 cut(s) 167
FaiI YATR 1 cut(s) 234
FalI AAGNNNNNCTT 2 cut(s) 64, 96
FaqI GGGAC 1 cut(s) 288
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 251, 305
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 1 cut(s) 121
HapII CCGG 1 cut(s) 140
HinfI GANTC 4 cut(s) 60, 86, 276, 329
HpaII CCGG 1 cut(s) 140
HphI GGTGA 2 cut(s) 253, 271
Hpy188I TCNGA 3 cut(s) 85, 229, 245
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 3 cut(s) 90, 184, 196
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 8, 285
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 176
Kzo9I GATC 1 cut(s) 253
LmnI GCTCC 2 cut(s) 23, 245
LpnPI CCDG 7 cut(s) 28, 89, 103, 153, 164, 234, 295
LweI GCATC 1 cut(s) 154
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 7 cut(s) 39, 207, 324, 327, 330, 333, 336
MflI RGATCY 1 cut(s) 253
MluCI AATT 1 cut(s) 323
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 3 cut(s) 31, 49, 163
MroXI GAANNNNTTC 1 cut(s) 79
MseI TTAA 1 cut(s) 349
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 141
NciI CCSGG 1 cut(s) 141
NdeII GATC 1 cut(s) 253
NmuCI GTSAC 1 cut(s) 259
NspV TTCGAA 1 cut(s) 327
PaqCI CACCTGC 1 cut(s) 290
PdmI GAANNNNTTC 1 cut(s) 79
PfeI GAWTC 3 cut(s) 60, 86, 329
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 349
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 1 cut(s) 141
SetI ASST 5 cut(s) 57, 156, 179, 250, 284
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 327
Sse9I AATT 1 cut(s) 323
SsiI CCGC 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 237, 327
TasI AATT 1 cut(s) 323
TfiI GAWTC 3 cut(s) 60, 86, 329
Tru1I TTAA 1 cut(s) 349
Tru9I TTAA 1 cut(s) 349
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 259
XapI RAATTY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.