MD12G1250500.v1.1
ERF Family

superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003853
Physical Location & Seq
Forward (+)
32064243 .. 32065100
858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1250500.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGCAGCTACAGCATCATCAAATGCTTCTGCAGAAGCACTTTCCAATCTAAGAATCGTAGTAGAAAGCAATCTCTCTGATTCACGATTATCTGAGTTGGGTATTAACTCATGGCCCAAATGGGGGTGTCCGCCTGGAAAGTACACATTGAAGTTTGACGCAGCGGAAACATGTTATCTGGTGAAAGGCAAAGTGAAGGTATACCCTAGAAAAGGCTCATCATCATCATCGTCTTCAGATTTTGTAGAGTTTGGTGCTGGGGACCTTGTGACCATTCCCAAGGGAGTCAGTTGCATATGGGACGTCTCAGTTGCTATCGATAAACACTACAAATTCGATTCTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

116

Amino Acids

12.31

Weight (kDa)

7.67

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 31 - 110 1.6e-24 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 306
AccI GTMKAC 1 cut(s) 201
AciI CCGC 2 cut(s) 131, 164
AcsI RAATTY 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 122, 123, 212
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 310
AoxI GGCC 1 cut(s) 113
ApeKI GCWGC 2 cut(s) 5, 161
ApoI RAATTY 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 114, 262
AsuHPI GGTGA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 17, 173
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 2 cut(s) 9, 30
BisI GCNGC 2 cut(s) 6, 162
BlsI GCNGC 2 cut(s) 7, 163
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 114, 262
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 225
Bsa29I ATCGAT 1 cut(s) 318
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 123, 212
BseBI CCWGG 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 279
BseLI CCNNNNNNNGG 3 cut(s) 122, 123, 212
BseMII CTCAG 2 cut(s) 84, 321
BseXI GCAGC 2 cut(s) 17, 173
BseYI CCCAGC 1 cut(s) 257
BshFI GGCC 1 cut(s) 115
BshVI ATCGAT 1 cut(s) 318
BslFI GGGAC 2 cut(s) 275, 314
BslI CCNNNNNNNGG 3 cut(s) 122, 123, 212
BsmAI GTCTC 1 cut(s) 310
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 275, 314
BsnI GGCC 1 cut(s) 115
BspACI CCGC 2 cut(s) 131, 164
BspANI GGCC 1 cut(s) 115
BspCNI CTCAG 2 cut(s) 85, 320
BspDI ATCGAT 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 263
BspMAI CTGCAG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 279
BssNAI GTATAC 1 cut(s) 202
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 279
Bst1107I GTATAC 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 303
BstDEI CTNAG 3 cut(s) 50, 93, 307
BstENI CCTNNNNNAGG 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 310
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 2 cut(s) 9, 30
BstV1I GCAGC 2 cut(s) 17, 173
BstV2I GAAGAC 1 cut(s) 225
BstZ17I GTATAC 1 cut(s) 202
Bsu15I ATCGAT 1 cut(s) 318
BsuRI GGCC 1 cut(s) 115
BsuTUI ATCGAT 1 cut(s) 318
Cfr13I GGNCC 2 cut(s) 114, 262
ClaI ATCGAT 1 cut(s) 318
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 142
CviAII CATG 2 cut(s) 111, 171
CviJI RGCY 3 cut(s) 8, 115, 216
CviKI_1 RGCY 3 cut(s) 8, 115, 216
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 3 cut(s) 50, 93, 307
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 279
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 2 cut(s) 114, 174
FaiI YATR 5 cut(s) 112, 172, 202, 296, 298
FaqI GGGAC 2 cut(s) 275, 314
FatI CATG 2 cut(s) 110, 170
FauNDI CATATG 1 cut(s) 296
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 6, 162
Fsp4HI GCNGC 2 cut(s) 6, 162
FspBI CTAG 1 cut(s) 207
GluI GCNGC 2 cut(s) 6, 162
GsaI CCCAGC 1 cut(s) 261
HaeIII GGCC 1 cut(s) 115
HgaI GACGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 2 cut(s) 114, 174
HinfI GANTC 4 cut(s) 54, 80, 285, 338
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 144, 202
Hpy188I TCNGA 3 cut(s) 79, 94, 238
Hpy188III TCNNGA 2 cut(s) 84, 345
Hpy8I GTNNAC 2 cut(s) 144, 202
HpyAV CCTTC 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 32, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 3 cut(s) 50, 93, 307
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 2 cut(s) 114, 174
LpnPI CCDG 4 cut(s) 120, 147, 164, 243
Lsp1109I GCAGC 2 cut(s) 17, 173
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 207
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 1 cut(s) 268
MboII GAAGA 2 cut(s) 225, 333
MluCI AATT 1 cut(s) 332
MlyI GAGTC 1 cut(s) 294
MseI TTAA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeI CATATG 1 cut(s) 296
NlaIII CATG 2 cut(s) 114, 174
NlaIV GGNNCC 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 174
PciI ACATGT 1 cut(s) 170
PfeI GAWTC 3 cut(s) 54, 80, 338
PkrI GCNGC 2 cut(s) 7, 163
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PpuMI RGGWCCY 1 cut(s) 262
PscI ACATGT 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 133
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 263
PspPI GGNCC 2 cut(s) 114, 262
PspPPI RGGWCCY 1 cut(s) 262
PstI CTGCAG 1 cut(s) 34
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 6, 162
Sau96I GGNCC 2 cut(s) 114, 262
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 1 cut(s) 135
SetI ASST 4 cut(s) 10, 201, 267, 306
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 2 cut(s) 9, 30
SinI GGWCC 1 cut(s) 262
Sse9I AATT 1 cut(s) 332
SsiI CCGC 2 cut(s) 131, 164
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 279
TaiI ACGT 1 cut(s) 306
TaqI TCGA 2 cut(s) 318, 336
TasI AATT 1 cut(s) 332
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 3 cut(s) 54, 80, 338
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 2 cut(s) 5, 161
Tsp45I GTSAC 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 262
XagI CCTNNNNNAGG 1 cut(s) 210
XapI RAATTY 1 cut(s) 332
XceI RCATGY 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 1 cut(s) 207
ZraI GACGTC 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.