MD04G1232900.v1.1
ERF Family

superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003853
Physical Location & Seq
Forward (+)
31213248 .. 31214309
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1232900.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGCAGCAACAACATCATCAAGTACTTCTGCCGAAGCACTTCCCAATCTAAGAATCGTAGTAGAAAGTAACCCTTCCAATTCACAATTATCTGAGTTGGGTATTAACTCATGGCCCAAATGGGGGTGTCCACCGGGAAAGTACACATTGAAGTTTGATGCAGAGGAGACATGTTATCTGGTGAAAGGCAAGGTGAAGGTTTACCCTAGAAAAGGCTCATCATCGTCATCTGATAAGGAGTTTGTAGAGTTTGGTGCTGGAGACCTTGTGACCATTCCCAAGGGACTCAGTTGCACATGGGACGTCTCAGTTGCTATTGATAAACACTACAAATTCGATTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

116

Amino Acids

12.46

Weight (kDa)

6.56

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 31 - 110 1e-25 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 306
AcsI RAATTY 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 2 cut(s) 25, 143
AfiI CCNNNNNNNGG 3 cut(s) 122, 123, 212
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 1 cut(s) 151
Alw26I GTCTC 3 cut(s) 161, 255, 310
AoxI GGCC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 332
Asp700I GAANNNNTTC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 114
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 193, 205
BbvI GCAGC 1 cut(s) 17
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 161, 255, 310
BfaI CTAG 1 cut(s) 207
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
BmcAI AGTACT 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 135
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 279
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 123, 212
BseDI CCNNGG 1 cut(s) 279
BseLI CCNNNNNNNGG 3 cut(s) 122, 123, 212
BseMII CTCAG 3 cut(s) 84, 301, 321
BseRI GAGGAG 1 cut(s) 179
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 2 cut(s) 297, 314
BslI CCNNNNNNNGG 3 cut(s) 122, 123, 212
BsmAI GTCTC 3 cut(s) 161, 255, 310
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 297, 314
BsnI GGCC 1 cut(s) 115
Bso31I GGTCTC 1 cut(s) 255
BspANI GGCC 1 cut(s) 115
BspCNI CTCAG 3 cut(s) 85, 300, 320
BspHI TCATGA 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 279
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 279
BstACI GRCGYC 1 cut(s) 303
BstDEI CTNAG 4 cut(s) 50, 93, 287, 307
BstENI CCTNNNNNAGG 1 cut(s) 210
BstMAI GTCTC 3 cut(s) 161, 255, 310
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 115
CciI TCATGA 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 24, 142
CviAII CATG 4 cut(s) 111, 171, 297, 345
CviJI RGCY 2 cut(s) 115, 216
CviKI_1 RGCY 2 cut(s) 115, 216
CviQI GTAC 2 cut(s) 24, 142
DdeI CTNAG 4 cut(s) 50, 93, 287, 307
Eco130I CCWWGG 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 255
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 279
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 4 cut(s) 114, 174, 300, 348
FaiI YATR 4 cut(s) 112, 172, 298, 346
FalI AAGNNNNNCTT 2 cut(s) 58, 90
FaqI GGGAC 2 cut(s) 297, 314
FatI CATG 4 cut(s) 110, 170, 296, 344
Fnu4HI GCNGC 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 207
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 1 cut(s) 115
HapII CCGG 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 4 cut(s) 114, 174, 300, 348
HinfI GANTC 3 cut(s) 54, 285, 338
HpaII CCGG 1 cut(s) 134
HphI GGTGA 2 cut(s) 193, 205
Hpy166II GTNNAC 3 cut(s) 131, 144, 202
Hpy188I TCNGA 2 cut(s) 94, 232
Hpy188III TCNNGA 1 cut(s) 345
Hpy8I GTNNAC 3 cut(s) 131, 144, 202
HpyAV CCTTC 2 cut(s) 84, 190
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 161, 294
HpyF3I CTNAG 4 cut(s) 50, 93, 287, 307
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 4 cut(s) 114, 174, 300, 348
LpnPI CCDG 3 cut(s) 147, 164, 243
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 207
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 68, 268
MboII GAAGA 1 cut(s) 333
MluCI AATT 3 cut(s) 79, 86, 332
MlyI GAGTC 1 cut(s) 279
MnlI CCTC 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 1 cut(s) 105
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 135
NciI CCSGG 1 cut(s) 135
NlaIII CATG 4 cut(s) 114, 174, 300, 348
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 174
PagI TCATGA 1 cut(s) 344
PciI ACATGT 1 cut(s) 170
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 2 cut(s) 54, 338
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 279
PscI ACATGT 1 cut(s) 170
PspPI GGNCC 1 cut(s) 114
RsaI GTAC 2 cut(s) 25, 143
RsaNI GTAC 2 cut(s) 24, 142
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 114
ScaI AGTACT 1 cut(s) 25
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 135
SetI ASST 4 cut(s) 195, 201, 267, 306
SfaNI GCATC 1 cut(s) 148
Sse9I AATT 3 cut(s) 79, 86, 332
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 279
TaiI ACGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 336
TasI AATT 3 cut(s) 79, 86, 332
TatI WGTACW 2 cut(s) 23, 141
TfiI GAWTC 2 cut(s) 54, 338
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 333
XagI CCTNNNNNAGG 1 cut(s) 210
XapI RAATTY 1 cut(s) 332
XceI RCATGY 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 1 cut(s) 207
ZraI GACGTC 1 cut(s) 304
ZrmI AGTACT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.