pycom05g17040

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003853
Physical Location & Seq
Reverse (-)
20306497 .. 20307135
639 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17040.1

Sequence Viewer

Length: 342 bp
ATGGCTGCAGAACCCTGCTCATCATCACCATCAAGTTCCTCATCATCTTCAAACCTGAGAATCACAGTTGAAAGAAACCCTTCTGAATCTCGCTTATCCCAACTGGACATCAAGTGCTGGCCCAAATGGGGTTGCTCGCCTGGGAAGTACCAGCTGAAGTTTGATGCGGAGGAGACGTGTTATTTGTTGAAGGGAAGAGTGAAGGCGTATTATCCAAAAGGGTCTTCGGAATATGTCGAGTTCGGAGCTGGAGATCTTGTCACCATCCCAAAGGGACTCACCTGCACTTGGGATGTTTCTCTTGCTGTTGATAAACACTACAAATTCGAATCCTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

114

Amino Acids

12.49

Weight (kDa)

7.58

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 33 - 107 5.1e-24 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 290
Acc36I ACCTGC 1 cut(s) 290
AciI CCGC 1 cut(s) 167
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 128, 288
AflIII ACRYGT 1 cut(s) 176
AgsI TTSAA 3 cut(s) 51, 71, 190
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 208, 240
AluBI AGCT 2 cut(s) 154, 248
AluI AGCT 2 cut(s) 154, 248
Alw26I GTCTC 1 cut(s) 167
AoxI GGCC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 323
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
Asp700I GAANNNNTTC 1 cut(s) 79
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 3 cut(s) 18, 253, 271
AsuII TTCGAA 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 216
BccI CCATC 2 cut(s) 37, 272
BciT130I CCWGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 290
BglII AGATCT 1 cut(s) 253
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 141
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 270
Bpu14I TTCGAA 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 140
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 288
Bse1I ACTGG 1 cut(s) 108
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 2 cut(s) 264, 298
BseLI CCNNNNNNNGG 2 cut(s) 128, 288
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 1 cut(s) 185
BsgI GTGCAG 1 cut(s) 268
BshFI GGCC 1 cut(s) 121
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 128, 288
BsmAI GTCTC 1 cut(s) 167
BsmBI CGTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 288
BsnI GGCC 1 cut(s) 121
Bsp119I TTCGAA 1 cut(s) 327
Bsp143I GATC 1 cut(s) 253
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 121
BspCNI CTCAG 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 290
BspT104I TTCGAA 1 cut(s) 327
BsrI ACTGG 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 1 cut(s) 253
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 190
BstBI TTCGAA 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 119, 137
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 2 cut(s) 264, 298
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 1 cut(s) 253
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BstSFI CTRYAG 1 cut(s) 6
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 121
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 2 cut(s) 264, 298
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 2 cut(s) 119, 137
Cfr13I GGNCC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 148
CviJI RGCY 4 cut(s) 5, 121, 154, 248
CviKI_1 RGCY 4 cut(s) 5, 121, 154, 248
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 56
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 167
FaiI YATR 1 cut(s) 234
FalI AAGNNNNNCTT 2 cut(s) 64, 96
FaqI GGGAC 1 cut(s) 288
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 251, 305
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 1 cut(s) 121
HinfI GANTC 4 cut(s) 60, 86, 276, 329
HphI GGTGA 3 cut(s) 18, 253, 271
Hpy188I TCNGA 3 cut(s) 85, 229, 245
HpyAV CCTTC 3 cut(s) 90, 184, 196
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 8, 285
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 176
Kzo9I GATC 1 cut(s) 253
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 9 cut(s) 28, 68, 89, 103, 126, 153, 164, 234, 295
LweI GCATC 1 cut(s) 154
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 4 cut(s) 39, 207, 216, 327
MflI RGATCY 1 cut(s) 253
MluCI AATT 1 cut(s) 323
MlyI GAGTC 1 cut(s) 270
MnlI CCTC 2 cut(s) 49, 163
MroXI GAANNNNTTC 1 cut(s) 79
MseI TTAA 1 cut(s) 340
MspA1I CMGCKG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
NdeII GATC 1 cut(s) 253
NmuCI GTSAC 1 cut(s) 259
NspV TTCGAA 1 cut(s) 327
PaqCI CACCTGC 1 cut(s) 290
PdmI GAANNNNTTC 1 cut(s) 79
PfeI GAWTC 3 cut(s) 60, 86, 329
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 340
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 1 cut(s) 141
SetI ASST 5 cut(s) 57, 156, 179, 250, 284
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 327
Sse9I AATT 1 cut(s) 323
SsiI CCGC 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 237, 327
TasI AATT 1 cut(s) 323
TfiI GAWTC 3 cut(s) 60, 86, 329
Tru1I TTAA 1 cut(s) 340
Tru9I TTAA 1 cut(s) 340
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 259
XapI RAATTY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.