FvH4_2g20230

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003853
Physical Location & Seq
Reverse (-)
17015712 .. 17016487
776 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20230.t2

Sequence Viewer

Length: 345 bp
ATGGCTGCAGAATATTCCTGCTCTTCATCTTCATCTTCTTCTTCTTCTTCTTCTTCCTCAAACCTAAGAATCACAGTTGAGAAAAACCCTTCTGAGTCTCGCTTATCTGAACTCGACATCAAGTGCTGGCCCAAATGGGGTTGTTCTCCTGGGAAGTACCAGCTGAAGTTTGATGCAGAGGAGACGTGTTACTTGCTCAAGGGAAGAGTGAAGGCATACCCGAAAGGATCATCGGAGTTCGTCGAGTTCGGAGCCGGCGACCTGGTTACGATCCCGAAGGGACTGAGTTGCACTTGGGATGTATCAATTGCTGTTGATAAACATTATAAATTCGAATCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

115

Amino Acids

12.5

Weight (kDa)

6.56

Isoelectric Point (pI)

59.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 51 - 114 1.2e-23 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AclWI GGATC 2 cut(s) 235, 265
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 185
AjiI CACGTC 1 cut(s) 186
AjnI CCWGG 2 cut(s) 148, 261
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
Alw26I GTCTC 2 cut(s) 102, 176
AlwI GGATC 2 cut(s) 235, 265
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 129
AsuII TTCGAA 1 cut(s) 333
BciT130I CCWGG 2 cut(s) 150, 263
BcoDI GTCTC 2 cut(s) 102, 176
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 150, 263
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 150, 263
BmsI GCATC 1 cut(s) 163
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse118I RCCGGY 1 cut(s) 254
BseBI CCWGG 2 cut(s) 150, 263
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMII CTCAG 2 cut(s) 84, 275
BseRI GAGGAG 1 cut(s) 194
BshFI GGCC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 2 cut(s) 102, 176
BsmBI CGTCTC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 1 cut(s) 130
Bsp119I TTCGAA 1 cut(s) 333
Bsp143I GATC 2 cut(s) 227, 270
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 2 cut(s) 85, 276
BspLI GGNNCC 1 cut(s) 253
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 2 cut(s) 235, 265
BspQI GCTCTTC 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 2 cut(s) 227, 270
Bst2UI CCWGG 2 cut(s) 150, 263
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 2 cut(s) 28, 199
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 128, 256
BstDEI CTNAG 3 cut(s) 65, 93, 284
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 230, 273
BstMAI GTCTC 2 cut(s) 102, 176
BstMBI GATC 2 cut(s) 227, 270
BstNI CCWGG 2 cut(s) 150, 263
BstSCI CCNGG 2 cut(s) 148, 261
BstSFI CTRYAG 1 cut(s) 6
BsuRI GGCC 1 cut(s) 130
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 1 cut(s) 304
Cac8I GCNNGC 2 cut(s) 128, 256
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 129
CsiI ACCWGGT 1 cut(s) 261
Csp6I GTAC 1 cut(s) 157
CviJI RGCY 4 cut(s) 5, 130, 163, 254
CviKI_1 RGCY 4 cut(s) 5, 130, 163, 254
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 3 cut(s) 65, 93, 284
DpnI GATC 2 cut(s) 229, 272
DpnII GATC 2 cut(s) 227, 270
Eam1104I CTCTTC 2 cut(s) 28, 199
EarI CTCTTC 2 cut(s) 28, 199
Eco57I CTGAAG 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 148, 261
Esp3I CGTCTC 1 cut(s) 176
FaiI YATR 2 cut(s) 217, 327
FaqI GGGAC 1 cut(s) 294
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 255
HinfI GANTC 3 cut(s) 69, 95, 335
HpaII CCGG 1 cut(s) 255
Hpy188I TCNGA 4 cut(s) 94, 109, 235, 251
Hpy188III TCNNGA 2 cut(s) 274, 342
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 3 cut(s) 99, 205, 271
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 3 cut(s) 8, 176, 291
HpyF3I CTNAG 3 cut(s) 65, 93, 284
HpySE526I ACGT 1 cut(s) 185
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 227, 270
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 1 cut(s) 251
LpnPI CCDG 8 cut(s) 31, 112, 135, 162, 173, 248, 268, 275
LweI GCATC 1 cut(s) 163
MabI ACCWGGT 1 cut(s) 261
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 2 cut(s) 188, 265
MalI GATC 2 cut(s) 229, 272
MboI GATC 2 cut(s) 227, 270
MfeI CAATTG 1 cut(s) 306
MluCI AATT 2 cut(s) 306, 329
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 2 cut(s) 67, 172
MroNI GCCGGC 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 163
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 2 cut(s) 150, 263
MunI CAATTG 1 cut(s) 306
MvaI CCWGG 2 cut(s) 150, 263
NaeI GCCGGC 1 cut(s) 256
NdeII GATC 2 cut(s) 227, 270
NgoMIV GCCGGC 1 cut(s) 254
NlaIV GGNNCC 1 cut(s) 253
NspV TTCGAA 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 69, 335
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 148, 261
PspGI CCWGG 2 cut(s) 148, 261
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 10
PvuII CAGCTG 1 cut(s) 163
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 28
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 227, 270
Sau96I GGNCC 1 cut(s) 129
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 150, 263
SetI ASST 4 cut(s) 66, 165, 188, 264
SexAI ACCWGGT 1 cut(s) 261
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 2 cut(s) 306, 329
SspI AATATT 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 148, 261
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 188
TaqI TCGA 3 cut(s) 114, 243, 333
TasI AATT 2 cut(s) 306, 329
TfiI GAWTC 2 cut(s) 69, 335
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 2 cut(s) 15, 21
XapI RAATTY 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.