Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005257

Physical Location & Seq

Genomic Coordinates Reverse (-)

1235209 .. 1238630

Gene Structure 3422 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G04580.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGTTAAGATCTTTAGGTCTTGGATTGCTTCTGTTTGCTCTTCTTGCTCTGGTTTCTGGTGATAACGATTACGTGAGCTGTAATTGTGACGACGAAGGTTTCTTAAGTGTCCACACCATCTTAGAATGTCAAAGAGTGAGTGATCTCTTAATAGCCATTGCCTATTTCTCAATACCTCTCGAGCTTCTCTACTTCATTAGCTTCTCCAATGTTCCTTTCAAATGGGTTCTTGTTCAATTCATTGCCTTTATTGTCCTATGTGGAATGACTCATTTGCTTAACGCTTGGACGTATTACGGACCACACTCTTTCCAGCTTATGCTTTGGCTTACCATCTTTAAATTCCTCACGGCGCTTGTCTCGTGTGCCACGGCTATCACGCTCTTGACTTTGATTCCGTTGCTGTTGAAATGGAAAGTTAGAGAGCTTTACTTGAAGCAGAATGTGTTGGAGCTTAATGAAGAGGTTGGATTGATGAAGAGGCAGAAGGAAATGAGTGTGCAGGTGAGAATGCTTACTCGGGAGATCAGGAAATCGCTCGATAAGCATATGATTCTGCGGACTACTCTCGTTGAGCTATCCAAGATTCTTGATTTACAGAACTCTGCTGTTTGGATGCCTAACGAGAACAGAACCGAGATGCATTTGACTCATGAGCTTAGAGCGAATCCGATGAGGAGTTTCAGGGTAATCCCGATTAATGATCCCGATGTTGTTCAGGTTAGGGAGACCAAAGTAGTGACAATTTTGAGGAAAAACTCTGTTCTTGCGGTTGAGAGCAGTGGTTGTGGCGGTTCGGAAGAGTTTGGACCGGTTGCTGCAATTCGGATGCCGATGCTTCACGGATTGAATTTCAAAGGAGGCACTCCCGAGTTTGTGGATACTCCTTATGCTATAATGGTTTTGGTACTTCCGAGTGCGAATTCACGGGTTTGGACCGATAAAGAGATCGAGATAGCGGAAGTTGTTGCTGATCAAGTAGCGGTTGCTATCTCTCATGCCTCAGTTCTTGAAGAATCACAGTTAATGAGAGAAAAACTCGGTATTCAAAACCGAGCTTTACTTCGAGCAAAACAGAACGCGATGATGGCAAGTCAAGCGAGGAACACTTGCCAGAAAGTGATGAGTCATGGAATGAGGAGACCAATGCACACAATTCTTGGTCTACTCTCCATGTTTCAGTCCGAAAGTATGAGTCTTGACCAGAAAATCATCGTTGATGCGCTCATGAAAACAAGCACAGTTCTCTCAGCTTTAATCAACGACGTGATTGACATTTCGCCTAAAGATAACGGGAAATCTGCATTAGAGGTTAAACGGTTCCAGCTACATTCCTTGATAAGAGAAGCCGCTTGTGTTGCTAAATGCTTAAGCGTTTACAAAGGTTACGGTTTTGAGATGGATGTTCAAACGCGGTTGCCTAATTTAGTAGTAGGAGATGAAAAGAGAACGTTTCAGCTTGTGATGTATATGCTTGGGTATATCTTGGATATGACTGATGGAGGCAAAACCGTTACGTTCCGAGTTATCTGTGAAGGTACTGGAACAAGTCAGGACAAGAGCAAAAGAGAAACTGGAATGTGGAAGTCCCATATGTCCGATGATTCCCTCGGTGTGAAATTTGAAGTTGAGATTAATGAGATTCAGAATCCTCCATTGGATGGTTCGGCTATGGCTATGAGACATATTCCGAACAGAAGATATCATAGCAATGGGATTAAGGAAGGCTTGAGCTTAGGCATGTGTAGAAAACTTGCACAGATGATGCAAGGAAACATATGGATATCACCTAAATCGCATGGACAAACACAGAGTATGCAATTAGTTCTGAGGTTTCAGACAAGACCATCGATTAGGAGATCAATCTTAGCAGGAAATGCTCCAGAGCTTCAACATCCGAATTCAAATTCCATTCTTCGTGGTCTAAGAATCACTCTAGCAGACGATGATGATGTCAACAGAACCGTGACTAAGAGACTTCTTGAGAAACTCGGCTGCGAAGTAACTGCTGTCTCGTCTGGATTTGAGTGTTTGAATGCGTTGTCAAATGTGGAAATGTCGTATAGAGTTGTGATTTTGGATCTGCAGATGCCGGAGATGGATGGTTTCGAAGTAGCAATGAAGATAAGGAAGTTTTGTGGACATCATTGGCCATTGATTATAGCTTTGACTGCTAGTACTGAAGATCATGTTAGGGAAAGATGTTTGCAAATGGGGATGAATGGTATGATCCAGAAACCGGTTCTTTTGCATGTTATGGCTTCTGAGCTTAGACGGGCTTTGCAGACCGCGAGCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

766

Amino Acids

86.25

Weight (kDa)

8.49

Isoelectric Point (pI)

41.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	40 - 137	5.2e-41	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	183 - 331	1.4e-11	GAF domain
HisKA	PF00512	369 - 432	1.5e-07	His Kinase A (phospho-acceptor) domain
Response_reg	PF00072	644 - 750	3.4e-21	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04580 AT3G04580
fragaria_vesca	FvH4_3g14281 FvH4_5g22480 FvH4_6g02340
malus_domestica	MD00G1114300.v1.1 MD12G1245100.v1.1
prunus_persica	Prupe.6G348000_v2.0.a1
pyrus_communis	pycom04g20230 pycom04g20240 pycom12g22480
rosa_chinensis	RchiOBHm_Chr3g0450101 RchiOBHm_Chr6g0274471
rosa_laevigata	RLG00000008654 RLG00000025756
rosa_multiflora	Rmu_sc0018882.1_g000003 Rmu_sc0035048.1_g000001 Rmu_sc0035049.1_g000001
rosa_roxburghii	Rroxscaffold_2G00117790 Rroxscaffold_3G00276360 Rroxscaffold_7G00200060
rosa_rugosa	Rorug02G0625400 Rorug02G0625500 Rorug04G0237900
rosa_samantha	Rh3AG024700 Rh3BG025300 Rh3CG024200 Rh3DG025300
rosa_wichuraiana	Rw3G001970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	493
Acc36I	ACCTGC	1 cut(s)	493
AccB7I	CCANNNNNTGG	1 cut(s)	1661
AccI	GTMKAC	1 cut(s)	1165
AccII	CGCG	3 cut(s)	1082, 1414, 2291
AciI	CCGC	8 cut(s)	559, 770, 792, 959, 983, 1350, 1414, 2289
AclI	AACGTT	1 cut(s)	1451
AclWI	GGATC	3 cut(s)	698, 2088, 2224
AcoI	YGGCCR	1 cut(s)	2150
AcsI	RAATTY	6 cut(s)	341, 850, 922, 1619, 1900, 1906
AcuI	CTGAAG	1 cut(s)	2202
AfaI	GTAC	3 cut(s)	909, 1540, 2179
AfiI	CCNNNNNNNGG	2 cut(s)	1661, 2239
AflII	CTTAAG	2 cut(s)	103, 1369
AgeI	ACCGGT	2 cut(s)	811, 2239
AjiI	CACGTC	1 cut(s)	1267
AjuI	GAANNNNNNNTTGG	4 cut(s)	200, 232, 1640, 1672
Alw26I	GTCTC	6 cut(s)	364, 722, 1135, 1675, 1969, 2017
AlwI	GGATC	3 cut(s)	698, 2088, 2224
Ama87I	CYCGRG	3 cut(s)	179, 519, 869
AoxI	GGCC	1 cut(s)	2150
ApeKI	GCWGC	2 cut(s)	818, 1995
ApoI	RAATTY	6 cut(s)	341, 850, 922, 1619, 1900, 1906
AseI	ATTAAT	2 cut(s)	699, 1635
AsiGI	ACCGGT	2 cut(s)	811, 2239
AspLEI	GCGC	2 cut(s)	355, 1225
AspS9I	GGNCC	3 cut(s)	299, 809, 936
AsuHPI	GGTGA	3 cut(s)	71, 517, 1779
AsuII	TTCGAA	1 cut(s)	2109
AvaI	CYCGRG	3 cut(s)	179, 519, 869
AvaII	GGWCC	3 cut(s)	299, 809, 936
BalI	TGGCCA	1 cut(s)	2152
BauI	CACGAG	1 cut(s)	361
BbvI	GCAGC	2 cut(s)	805, 1982
BccI	CCATC	9 cut(s)	125, 341, 1081, 1393, 1493, 1655, 1855, 2092, 2096
BceAI	ACGGC	2 cut(s)	366, 387
BcgI	CGANNNNNNTGC	2 cut(s)	1988, 2022
BciVI	GTATCC	1 cut(s)	874
BclI	TGATCA	1 cut(s)	973
BcoDI	GTCTC	6 cut(s)	364, 722, 1135, 1675, 1969, 2017
BfaI	CTAG	2 cut(s)	1937, 2175
BfmI	CTRYAG	1 cut(s)	2084
BfoI	RGCGCY	1 cut(s)	356
BfrI	CTTAAG	2 cut(s)	103, 1369
BfuAI	ACCTGC	1 cut(s)	493
BfuI	GTATCC	1 cut(s)	874
BglII	AGATCT	1 cut(s)	8
BisI	GCNGC	3 cut(s)	819, 1350, 1996
BlsI	GCNGC	3 cut(s)	820, 1351, 1997
BmcAI	AGTACT	1 cut(s)	2179
Bme18I	GGWCC	3 cut(s)	299, 809, 936
BmeT110I	CYCGRG	3 cut(s)	179, 519, 869
BmgBI	CACGTC	1 cut(s)	1267
BmgT120I	GGNCC	3 cut(s)	299, 809, 936
BmiI	GGNNCC	1 cut(s)	1322
BmsI	GCATC	7 cut(s)	606, 630, 819, 825, 1210, 1755, 2079
BplI	GAGNNNNNCTC	2 cut(s)	1023, 1055
BpmI	CTGGAG	1 cut(s)	1866
Bpu10I	CCTNAGC	1 cut(s)	1735
Bpu14I	TTCGAA	1 cut(s)	2109
BpuEI	CTTGAG	2 cut(s)	1750, 2003
Bsa29I	ATCGAT	1 cut(s)	1850
BsaAI	YACGTR	1 cut(s)	73
BsaI	GGTCTC	2 cut(s)	722, 1135
BsaJI	CCNNGG	2 cut(s)	369, 1609
BsaWI	WCCGGW	2 cut(s)	811, 2239
Bsc4I	CCNNNNNNNGG	2 cut(s)	1661, 2239
Bse118I	RCCGGY	2 cut(s)	811, 2239
Bse1I	ACTGG	2 cut(s)	1546, 1579
Bse3DI	GCAATG	4 cut(s)	156, 240, 1717, 2124
BseCI	ATCGAT	1 cut(s)	1850
BseDI	CCNNGG	2 cut(s)	369, 1609
BseGI	GGATG	7 cut(s)	621, 834, 1408, 1666, 1894, 2107, 2223
BseLI	CCNNNNNNNGG	2 cut(s)	1661, 2239
BseMI	GCAATG	4 cut(s)	156, 240, 1717, 2124
BseMII	CTCAG	4 cut(s)	1017, 1263, 1820, 2256
BseNI	ACTGG	2 cut(s)	1546, 1579
BseRI	GAGGAG	2 cut(s)	691, 1153
BseXI	GCAGC	2 cut(s)	805, 1982
BsgI	GTGCAG	1 cut(s)	521
Bsh1236I	CGCG	3 cut(s)	1082, 1414, 2291
BshFI	GGCC	1 cut(s)	2152
BshTI	ACCGGT	2 cut(s)	811, 2239
BshVI	ATCGAT	1 cut(s)	1850
BsiHKCI	CYCGRG	3 cut(s)	179, 519, 869
BsiSI	CCGG	3 cut(s)	812, 2093, 2240
BslFI	GGGAC	1 cut(s)	1573
BslI	CCNNNNNNNGG	2 cut(s)	1661, 2239
BsmAI	GTCTC	6 cut(s)	364, 722, 1135, 1675, 1969, 2017
BsmFI	GGGAC	1 cut(s)	1573
BsmI	GAATGC	2 cut(s)	516, 2041
BsnI	GGCC	1 cut(s)	2152
Bso31I	GGTCTC	2 cut(s)	722, 1135
BsoBI	CYCGRG	3 cut(s)	179, 519, 869
Bsp119I	TTCGAA	1 cut(s)	2109
BspACI	CCGC	8 cut(s)	559, 770, 792, 959, 983, 1350, 1414, 2289
BspANI	GGCC	1 cut(s)	2152
BspCNI	CTCAG	4 cut(s)	1016, 1262, 1821, 2257
BspDI	ATCGAT	1 cut(s)	1850
BspFNI	CGCG	3 cut(s)	1082, 1414, 2291
BspHI	TCATGA	2 cut(s)	652, 1227
BspLI	GGNNCC	1 cut(s)	1322
BspMAI	CTGCAG	1 cut(s)	2088
BspMI	ACCTGC	1 cut(s)	493
BspPI	GGATC	3 cut(s)	698, 2088, 2224
BspQI	GCTCTTC	1 cut(s)	45
BspT104I	TTCGAA	1 cut(s)	2109
BspTI	CTTAAG	2 cut(s)	103, 1369
BspTNI	GGTCTC	2 cut(s)	722, 1135
BsrDI	GCAATG	4 cut(s)	156, 240, 1717, 2124
BsrFI	RCCGGY	2 cut(s)	811, 2239
BsrI	ACTGG	2 cut(s)	1546, 1579
BssAI	RCCGGY	2 cut(s)	811, 2239
BssECI	CCNNGG	2 cut(s)	369, 1609
BssSI	CACGAG	1 cut(s)	361
Bst2BI	CACGAG	1 cut(s)	361
Bst4CI	ACNGT	6 cut(s)	1023, 1243, 1320, 1391, 1513, 1966
Bst6I	CTCTTC	4 cut(s)	45, 456, 473, 795
BstAFI	CTTAAG	2 cut(s)	103, 1369
BstAPI	GCANNNNNTGC	1 cut(s)	1877
BstBAI	YACGTR	1 cut(s)	73
BstBI	TTCGAA	1 cut(s)	2109
BstC8I	GCNNGC	1 cut(s)	2293
BstDSI	CCRYGG	1 cut(s)	369
BstF5I	GGATG	7 cut(s)	621, 834, 1408, 1666, 1894, 2107, 2223
BstFNI	CGCG	3 cut(s)	1082, 1414, 2291
BstH2I	RGCGCY	1 cut(s)	356
BstHHI	GCGC	2 cut(s)	355, 1225
BstMAI	GTCTC	6 cut(s)	364, 722, 1135, 1675, 1969, 2017
BstMWI	GCNNNNNNNGC	7 cut(s)	44, 544, 1088, 1097, 1358, 1877, 2171
BstNSI	RCATGY	2 cut(s)	1744, 2255
BstSFI	CTRYAG	1 cut(s)	2084
BstUI	CGCG	3 cut(s)	1082, 1414, 2291
BstV1I	GCAGC	2 cut(s)	805, 1982
BstX2I	RGATCY	2 cut(s)	8, 2080
BstYI	RGATCY	2 cut(s)	8, 2080
Bsu15I	ATCGAT	1 cut(s)	1850
BsuI	GTATCC	1 cut(s)	874
BsuRI	GGCC	1 cut(s)	2152
BsuTUI	ATCGAT	1 cut(s)	1850
BtgI	CCRYGG	1 cut(s)	369
BtgZI	GCGATG	1 cut(s)	1097
BtrI	CACGTC	1 cut(s)	1267
BtsCI	GGATG	7 cut(s)	621, 834, 1408, 1666, 1894, 2107, 2223
BtsI	GCAGTG	1 cut(s)	787
BtsIMutI	CAGTG	1 cut(s)	787
BveI	ACCTGC	1 cut(s)	493
Cac8I	GCNNGC	1 cut(s)	2293
CciI	TCATGA	2 cut(s)	652, 1227
CfoI	GCGC	2 cut(s)	355, 1225
Cfr10I	RCCGGY	2 cut(s)	811, 2239
Cfr13I	GGNCC	3 cut(s)	299, 809, 936
ClaI	ATCGAT	1 cut(s)	1850
Csp6I	GTAC	3 cut(s)	908, 1539, 2178
CspAI	ACCGGT	2 cut(s)	811, 2239
CviAII	CATG	9 cut(s)	653, 998, 1130, 1174, 1228, 1741, 1799, 2189, 2252
CviQI	GTAC	3 cut(s)	908, 1539, 2178
DraI	TTTAAA	1 cut(s)	340
EaeI	YGGCCR	1 cut(s)	2150
Eam1104I	CTCTTC	4 cut(s)	45, 456, 473, 795
EarI	CTCTTC	4 cut(s)	45, 456, 473, 795
Eco31I	GGTCTC	2 cut(s)	722, 1135
Eco32I	GATATC	2 cut(s)	1703, 1785
Eco47I	GGWCC	3 cut(s)	299, 809, 936
Eco57I	CTGAAG	1 cut(s)	2202
Eco88I	CYCGRG	3 cut(s)	179, 519, 869
EcoRI	GAATTC	2 cut(s)	922, 1900
EcoRV	GATATC	2 cut(s)	1703, 1785
EcoT22I	ATGCAT	1 cut(s)	645
FaeI	CATG	9 cut(s)	656, 1001, 1133, 1177, 1231, 1744, 1802, 2192, 2255
FalI	AAGNNNNNCTT	2 cut(s)	1712, 1744
FaqI	GGGAC	1 cut(s)	1573
FatI	CATG	9 cut(s)	652, 997, 1129, 1173, 1227, 1740, 1798, 2188, 2251
FauNDI	CATATG	3 cut(s)	549, 1593, 1778
FbaI	TGATCA	1 cut(s)	973
FblI	GTMKAC	1 cut(s)	1165
Fnu4HI	GCNGC	3 cut(s)	819, 1350, 1996
FokI	GGATG	7 cut(s)	628, 841, 1415, 1673, 1881, 2114, 2230
Fsp4HI	GCNGC	3 cut(s)	819, 1350, 1996
FspBI	CTAG	2 cut(s)	1937, 2175
GlaI	GCGC	2 cut(s)	354, 1224
GluI	GCNGC	3 cut(s)	819, 1350, 1996
GsuI	CTGGAG	1 cut(s)	1866
HaeII	RGCGCY	1 cut(s)	356
HaeIII	GGCC	1 cut(s)	2152
HapII	CCGG	3 cut(s)	812, 2093, 2240
HhaI	GCGC	2 cut(s)	355, 1225
Hin1II	CATG	9 cut(s)	656, 1001, 1133, 1177, 1231, 1744, 1802, 2192, 2255
Hin6I	GCGC	2 cut(s)	353, 1223
HinP1I	GCGC	2 cut(s)	353, 1223
HincII	GTYRAC	1 cut(s)	1957
HindII	GTYRAC	1 cut(s)	1957
HpaII	CCGG	3 cut(s)	812, 2093, 2240
HphI	GGTGA	3 cut(s)	71, 517, 1779
Hpy166II	GTNNAC	5 cut(s)	112, 1166, 1378, 1957, 2141
Hpy8I	GTNNAC	5 cut(s)	112, 1166, 1378, 1957, 2141
Hpy99I	CGWCG	2 cut(s)	95, 1268
HpyAV	CCTTC	4 cut(s)	89, 481, 1529, 1718
HpyCH4III	ACNGT	6 cut(s)	1023, 1243, 1320, 1391, 1513, 1966
HpyCH4IV	ACGT	5 cut(s)	72, 290, 1266, 1451, 1517
HpyF10VI	GCNNNNNNNGC	7 cut(s)	44, 544, 1088, 1097, 1358, 1877, 2171
HpySE526I	ACGT	5 cut(s)	72, 290, 1266, 1451, 1517
Hsp92II	CATG	9 cut(s)	656, 1001, 1133, 1177, 1231, 1744, 1802, 2192, 2255
HspAI	GCGC	2 cut(s)	353, 1223
Ksp22I	TGATCA	1 cut(s)	973
LguI	GCTCTTC	1 cut(s)	45
LmnI	GCTCC	2 cut(s)	451, 1885
Lsp1109I	GCAGC	2 cut(s)	805, 1982
LweI	GCATC	7 cut(s)	606, 630, 819, 825, 1210, 1755, 2079
MaeI	CTAG	2 cut(s)	1937, 2175
MaeII	ACGT	5 cut(s)	72, 290, 1266, 1451, 1517
MaeIII	GTNAC	6 cut(s)	86, 739, 1385, 1513, 1966, 2002
MboII	GAAGA	9 cut(s)	32, 473, 490, 812, 1025, 1710, 1907, 2134, 2195
MflI	RGATCY	2 cut(s)	8, 2080
MlsI	TGGCCA	1 cut(s)	2152
MluNI	TGGCCA	1 cut(s)	2152
MlyI	GAGTC	4 cut(s)	262, 643, 1135, 1204
MmeI	TCCRAC	2 cut(s)	429, 448
Mox20I	TGGCCA	1 cut(s)	2152
Mph1103I	ATGCAT	1 cut(s)	645
MscI	TGGCCA	1 cut(s)	2152
MslI	CAYNNNNRTG	1 cut(s)	1710
Msp20I	TGGCCA	1 cut(s)	2152
MspCI	CTTAAG	2 cut(s)	103, 1369
MspI	CCGG	3 cut(s)	812, 2093, 2240
Mva1269I	GAATGC	2 cut(s)	516, 2041
MvnI	CGCG	3 cut(s)	1082, 1414, 2291
MwoI	GCNNNNNNNGC	7 cut(s)	44, 544, 1088, 1097, 1358, 1877, 2171
NdeI	CATATG	3 cut(s)	549, 1593, 1778
NlaIII	CATG	9 cut(s)	656, 1001, 1133, 1177, 1231, 1744, 1802, 2192, 2255
NlaIV	GGNNCC	1 cut(s)	1322
NmeAIII	GCCGAG	1 cut(s)	1971
NmuCI	GTSAC	3 cut(s)	86, 739, 1966
NsiI	ATGCAT	1 cut(s)	645
NspI	RCATGY	2 cut(s)	1744, 2255
NspV	TTCGAA	1 cut(s)	2109
PaeR7I	CTCGAG	1 cut(s)	179
PagI	TCATGA	2 cut(s)	652, 1227
PaqCI	CACCTGC	1 cut(s)	493
PciSI	GCTCTTC	1 cut(s)	45
PctI	GAATGC	2 cut(s)	516, 2041
PfeI	GAWTC	9 cut(s)	394, 553, 586, 667, 1016, 1604, 1642, 1648, 1929
PflMI	CCANNNNNTGG	1 cut(s)	1661
PinAI	ACCGGT	2 cut(s)	811, 2239
PkrI	GCNGC	3 cut(s)	820, 1351, 1997
PleI	GAGTC	4 cut(s)	262, 643, 1134, 1203
PpsI	GAGTC	4 cut(s)	262, 643, 1134, 1203
Ppu21I	YACGTR	1 cut(s)	73
PshBI	ATTAAT	2 cut(s)	699, 1635
Psp1406I	AACGTT	1 cut(s)	1451
PspN4I	GGNNCC	1 cut(s)	1322
PspPI	GGNCC	3 cut(s)	299, 809, 936
PstI	CTGCAG	1 cut(s)	2088
PsuI	RGATCY	2 cut(s)	8, 2080
RsaI	GTAC	3 cut(s)	909, 1540, 2179
RsaNI	GTAC	3 cut(s)	908, 1539, 2178
RseI	CAYNNNNRTG	1 cut(s)	1710
SapI	GCTCTTC	1 cut(s)	45
SatI	GCNGC	3 cut(s)	819, 1350, 1996
Sau96I	GGNCC	3 cut(s)	299, 809, 936
ScaI	AGTACT	1 cut(s)	2179
SchI	GAGTC	4 cut(s)	262, 643, 1135, 1204
SfaNI	GCATC	7 cut(s)	606, 630, 819, 825, 1210, 1755, 2079
SfcI	CTRYAG	1 cut(s)	2084
Sfr274I	CTCGAG	1 cut(s)	179
SfuI	TTCGAA	1 cut(s)	2109
SinI	GGWCC	3 cut(s)	299, 809, 936
SlaI	CTCGAG	1 cut(s)	179
SmiMI	CAYNNNNRTG	1 cut(s)	1710
SmlI	CTYRAG	5 cut(s)	103, 179, 1369, 1729, 1982
SmoI	CTYRAG	5 cut(s)	103, 179, 1369, 1729, 1982
SsiI	CCGC	8 cut(s)	559, 770, 792, 959, 983, 1350, 1414, 2289
SspMI	CTAG	2 cut(s)	1937, 2175
TaaI	ACNGT	6 cut(s)	1023, 1243, 1320, 1391, 1513, 1966
TaiI	ACGT	5 cut(s)	75, 293, 1269, 1454, 1520
TaqI	TCGA	6 cut(s)	180, 540, 951, 1066, 1850, 2109
TaqII	GACCGA	1 cut(s)	953
TatI	WGTACW	1 cut(s)	2177
TauI	GCSGC	1 cut(s)	1352
TfiI	GAWTC	9 cut(s)	394, 553, 586, 667, 1016, 1604, 1642, 1648, 1929
TscAI	CASTG	1 cut(s)	787
TseFI	GTSAC	3 cut(s)	86, 739, 1966
TseI	GCWGC	2 cut(s)	818, 1995
Tsp45I	GTSAC	3 cut(s)	86, 739, 1966
TspDTI	ATGAA	8 cut(s)	184, 229, 474, 491, 1244, 1455, 2135, 2234
TspGWI	ACGGA	3 cut(s)	312, 387, 858
TspRI	CASTG	1 cut(s)	787
Van91I	CCANNNNNTGG	1 cut(s)	1661
Vha464I	CTTAAG	2 cut(s)	103, 1369
VpaK11BI	GGWCC	3 cut(s)	299, 809, 936
VspI	ATTAAT	2 cut(s)	699, 1635
XapI	RAATTY	6 cut(s)	341, 850, 922, 1619, 1900, 1906
XceI	RCATGY	2 cut(s)	1744, 2255
XhoI	CTCGAG	1 cut(s)	179
XmiI	GTMKAC	1 cut(s)	1165
XspI	CTAG	2 cut(s)	1937, 2175
ZrmI	AGTACT	1 cut(s)	2179
Zsp2I	ATGCAT	1 cut(s)	645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.