Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005257

Physical Location & Seq

Genomic Coordinates Reverse (-)

24269911 .. 24281456

Gene Structure 11546 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1114300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGTTAAGAGCATTAGCAATTGGATTGTTGGTTTCGTGTTTCGTTACATTAGTATCGGGCAGTGATCATGACTTTGCACATTGTAATTGTGATGAGGAGGGTTTTTGGAGCATTCCAAACATTATAGAGGGACAAAGAGTGAGCGATTTCTTGATTGCAGTTGCATATTTTTCAATCCCTATAGAACTCCTTTACTTTGTCAGCTGCTCGAATTTTCCGTTCAAATGGGTTCTCCTGCAGTTTATTGCATTCATTGTACTCTGTGGCTTGACCCATTTGCTAAATGCTTGGACGTATTATGGCCGTTACACGTTCCAACTGATGCTGTCCCTCACCATTGCCAAGTTCCTCACGGCCTTGGTCTCCTGCGCAACCGCAATAACCCTTTTAACTCTGTTCCCTATGATTCTCAAAGTAAAAGTGAGAGAATTGTTCTTGAGGCAAAATGTGATGGAATTAGACCAAGAGGTTGGGATGATGAAGATACAGAAGGAAGCGAGTTGGCATGTTCGAATGCTGACCCAGGAAATTAGGAAGTCGCTTGACAAGCATACCATATTGTATACAACTCTGGTTGAGCTTTCAAAGACGTTAGACTTGCATAACTGTGCAGTTTGGATGCTGAATGAGAAAAGAGGAGAGATGAACCTGACCCATGAGTTGAAAGTGAGTACTTCGCGAAAGTATCGCCATTCTATCCCAATTAATGACCCAGATGTGTTGGAGATAAGGGAGAGCGAAAGGGTAATGATCCTGAGACCTGATTCAGCGCTGGGATCTGCAAGTAGCGGTGAGTCTGGTGAATCTGGTGCTGTAGCAGCAATCCGGATGCCAATGCTTCAGGCTTCAAATTTCAAAGGGGGTACACCGCAGTTGGTTGATACTCATTATGCTATATTGGTTTTGGTTCTTCCGATTGCAAATTCTGGAGGTTGGAGCCATCATGAGCTGGAGATAGTGGAAGTTGTGGCTGACCAGGTCGCCGTGGCTCTATCCCATGCTGCAGTACTTGAAGAGTCTCAACTAATGAGGGAGAAACTGGGTGAACAAAATCGAGCGTTGCAACAAGCTAAGAAGAATGCAATGATGGCTAGCCAAGCAAGGCACTCGGTTCAGAAGGTAATGAGTCATGGAATGAGAGGGCCAATGCATACGATTTTGGGTTTGCTTTCGATGTTTCAAGAGAATTTAAGTTTTGAACAGAGCATTATTGTGGACACAACGGCAAAAACTAGTTATGTTCTCTGCACTTTGATAAATGATGTGATGGAGATGTCAGCAAAAGATAACGGAAGGTTCCTATTGGAGATGAAACCGTTTGAACTACATTCTATGATCAAGGAAGCTGCTTGCCTTGCCAAATGCTTGTGTATGTATAAAGGCTTTAGCTTTGAAATTGATGTTCAAAGCTCATTGCCTAATCAGGTGATAGGGGACGAAAAAAGGACTTTCCAAGTGCTCTTGCATATGATTGGGTACCTATTGAGCACCTATAATGGTGGGGGGACTGTCATCTTTCGGGCTATTTCTGAAAGTGGTAGTGATGGCCCTGATGATAGATTGCAAGGAATGTGGAGAACAGGTATTCCAGATGAGTACATTTGTATAAAGTTTGAATTTGAGATTAGTGAGGCAAGTTCTTATCCCGGCGGATTAGTAGCATTGACACACGATGATGGTAGGCGGCATGACAATGATGAAATCAAAAGGGGCTTGAGCTTCAGCATTTGTAAGAAGATTGTTCAGATGATGCAAGGCAATATCTGTATATCCATGAATCCGGTGGATTTTGCAGAAAGCATGACACTTGTTCTCAGGTTTCAAATCGTCTCGTCTACAGGGAGATCTATATATCCCCTCGGAAATACATTGGAGCAACCAAATTCCAACTCTCAGTTCAGTGGCCTTTGGGTTATAGTAGCGGATGATGATAGCGTAAACAGGACTGTGACCAGTAAGCTGCTCGAGAAACTTGGCTGTCAAGTTACTGCTGTGTCTTCTGGGTTTGAATGCCTGAGTGCCCTTACTGCCGCCAAAAACTCTTTTCAAATTGTTGTTTTGGATCTCCACATGCCGGGAATGGATGGGTTCGAAGTGGCAATGAGAATCAGGAAGTTCTGCAGCCGTAGTTGGCCATCGATCATTGCCCTCACGGCAAGTGCGGAGGAGCATATATGGGAGAGATGTCTGCAGCTGGGAATGAACGGATTGATTCGAAAACCGGTTCTTTTGCAAGGAATGGCGGATGAACTTTGTCGAGTTCTGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

757

Amino Acids

84.47

Weight (kDa)

5.99

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	41 - 139	4.1e-41	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	183 - 331	1.1e-10	GAF domain
Response_reg	PF00072	638 - 744	2.5e-21	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04580 AT3G04580
fragaria_vesca	FvH4_3g14281 FvH4_5g22480 FvH4_6g02340
malus_domestica	MD00G1114300.v1.1 MD12G1245100.v1.1
prunus_persica	Prupe.6G348000_v2.0.a1
pyrus_communis	pycom04g20230 pycom04g20240 pycom12g22480
rosa_chinensis	RchiOBHm_Chr3g0450101 RchiOBHm_Chr6g0274471
rosa_laevigata	RLG00000008654 RLG00000025756
rosa_multiflora	Rmu_sc0018882.1_g000003 Rmu_sc0035048.1_g000001 Rmu_sc0035049.1_g000001
rosa_roxburghii	Rroxscaffold_2G00117790 Rroxscaffold_3G00276360 Rroxscaffold_7G00200060
rosa_rugosa	Rorug02G0625400 Rorug02G0625500 Rorug04G0237900
rosa_samantha	Rh3AG024700 Rh3BG025300 Rh3CG024200 Rh3DG025300
rosa_wichuraiana	Rw3G001970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	370
Acc65I	GGTACC	1 cut(s)	1476
AccB1I	GGYRCC	1 cut(s)	1476
AccI	GTMKAC	2 cut(s)	563, 1835
AccII	CGCG	1 cut(s)	679
AccIII	TCCGGA	1 cut(s)	825
AciI	CCGC	9 cut(s)	375, 789, 869, 1650, 1684, 1922, 2031, 2162, 2243
AclWI	GGATC	3 cut(s)	745, 784, 2070
AcoI	YGGCCR	2 cut(s)	301, 2132
AcsI	RAATTY	6 cut(s)	211, 850, 922, 1186, 1616, 1882
AcuI	CTGAAG	2 cut(s)	824, 1705
AfaI	GTAC	6 cut(s)	258, 673, 865, 1008, 1478, 1598
AfeI	AGCGCT	1 cut(s)	771
AfiI	CCNNNNNNNGG	1 cut(s)	2074
AflIII	ACRYGT	1 cut(s)	309
AgeI	ACCGGT	1 cut(s)	2221
AhlI	ACTAGT	1 cut(s)	1232
AjnI	CCWGG	2 cut(s)	522, 975
AjuI	GAANNNNNNNTTGG	2 cut(s)	1880, 1912
AloI	GAACNNNNNNTCC	2 cut(s)	1570, 1602
Alw21I	GWGCWC	2 cut(s)	1461, 1490
Alw26I	GTCTC	4 cut(s)	367, 751, 1023, 1834
AlwI	GGATC	3 cut(s)	745, 784, 2070
Ama87I	CYCGRG	1 cut(s)	1964
Aor13HI	TCCGGA	1 cut(s)	825
Aor51HI	AGCGCT	1 cut(s)	771
AoxI	GGCC	6 cut(s)	301, 354, 1142, 1546, 1903, 2132
ApeKI	GCWGC	7 cut(s)	204, 818, 1001, 1346, 1960, 2121, 2191
ApoI	RAATTY	6 cut(s)	211, 850, 922, 1186, 1616, 1882
AseI	ATTAAT	1 cut(s)	705
AsiGI	ACCGGT	1 cut(s)	2221
Asp718I	GGTACC	1 cut(s)	1476
AspLEI	GCGC	2 cut(s)	371, 772
AspS9I	GGNCC	2 cut(s)	1142, 1547
AsuC2I	CCSGG	2 cut(s)	1647, 2076
AsuHPI	GGTGA	5 cut(s)	325, 803, 812, 1055, 1438
AsuII	TTCGAA	3 cut(s)	511, 2091, 2215
AsuNHI	GCTAGC	1 cut(s)	1091
AvaI	CYCGRG	1 cut(s)	1964
BaeGI	GKGCMC	1 cut(s)	2023
BaeI	ACNNNNGTAYC	2 cut(s)	36, 69
BalI	TGGCCA	1 cut(s)	2134
BanI	GGYRCC	1 cut(s)	1476
BbsI	GAAGAC	1 cut(s)	1989
Bbv12I	GWGCWC	2 cut(s)	1461, 1490
BbvI	GCAGC	7 cut(s)	191, 830, 988, 1333, 1947, 2133, 2203
BccI	CCATC	8 cut(s)	445, 948, 1081, 1261, 1538, 1670, 2078, 2143
BceAI	ACGGC	6 cut(s)	288, 369, 968, 1239, 2109, 2169
BcgI	CGANNNNNNTGC	2 cut(s)	1089, 1123
BciT130I	CCWGG	2 cut(s)	524, 977
BclI	TGATCA	2 cut(s)	64, 1335
BcnI	CCSGG	2 cut(s)	1647, 2076
BcoDI	GTCTC	4 cut(s)	367, 751, 1023, 1834
BcuI	ACTAGT	1 cut(s)	1232
BfaI	CTAG	2 cut(s)	1092, 1233
BfmI	CTRYAG	7 cut(s)	180, 236, 813, 1002, 1836, 2119, 2189
BfoI	RGCGCY	1 cut(s)	773
BglI	GCCNNNNNGGC	1 cut(s)	2153
BglII	AGATCT	1 cut(s)	1844
BisI	GCNGC	9 cut(s)	205, 819, 1002, 1347, 1685, 1961, 2031, 2122, 2192
BlsI	GCNGC	9 cut(s)	206, 820, 1003, 1348, 1686, 1962, 2032, 2123, 2193
BmcAI	AGTACT	2 cut(s)	673, 1008
Bme1390I	CCNGG	4 cut(s)	524, 977, 1647, 2076
BmeT110I	CYCGRG	1 cut(s)	1964
BmgT120I	GGNCC	2 cut(s)	1142, 1547
BmiI	GGNNCC	3 cut(s)	938, 1298, 1478
BmrFI	CCNGG	4 cut(s)	524, 977, 1647, 2076
BmrI	ACTGGG	1 cut(s)	1049
BmsI	GCATC	4 cut(s)	312, 609, 819, 1740
BmtI	GCTAGC	1 cut(s)	1095
BmuI	ACTGGG	1 cut(s)	1049
BpiI	GAAGAC	1 cut(s)	1989
BpmI	CTGGAG	2 cut(s)	948, 971
Bpu14I	TTCGAA	3 cut(s)	511, 2091, 2215
BpuEI	CTTGAG	2 cut(s)	457, 1735
BpuMI	CCSGG	2 cut(s)	1647, 2076
Bsa29I	ATCGAT	1 cut(s)	2138
BsaI	GGTCTC	2 cut(s)	367, 751
BsaJI	CCNNGG	4 cut(s)	357, 522, 984, 1858
BsaWI	WCCGGW	3 cut(s)	825, 1780, 2221
BsaXI	ACNNNNNCTCC	2 cut(s)	1567, 1597
Bsc4I	CCNNNNNNNGG	1 cut(s)	2074
Bse118I	RCCGGY	1 cut(s)	2221
Bse1I	ACTGG	2 cut(s)	1044, 1953
Bse3DI	GCAATG	5 cut(s)	336, 1089, 1412, 2106, 2142
BseAI	TCCGGA	1 cut(s)	825
BseBI	CCWGG	2 cut(s)	524, 977
BseCI	ATCGAT	1 cut(s)	2138
BseDI	CCNNGG	4 cut(s)	357, 522, 984, 1858
BseGI	GGATG	6 cut(s)	480, 624, 834, 1930, 2089, 2251
BseLI	CCNNNNNNNGG	1 cut(s)	2074
BseMI	GCAATG	5 cut(s)	336, 1089, 1412, 2106, 2142
BseMII	CTCAG	4 cut(s)	746, 1828, 1907, 2006
BseNI	ACTGG	2 cut(s)	1044, 1953
BseRI	GAGGAG	3 cut(s)	110, 651, 2180
BseSI	GKGCMC	1 cut(s)	2023
BseXI	GCAGC	7 cut(s)	191, 830, 988, 1333, 1947, 2133, 2203
BseYI	CCCAGC	2 cut(s)	772, 2194
BsgI	GTGCAG	2 cut(s)	630, 1231
Bsh1236I	CGCG	1 cut(s)	679
BshFI	GGCC	6 cut(s)	303, 356, 1144, 1548, 1905, 2134
BshNI	GGYRCC	1 cut(s)	1476
BshTI	ACCGGT	1 cut(s)	2221
BshVI	ATCGAT	1 cut(s)	2138
BsiHKAI	GWGCWC	2 cut(s)	1461, 1490
BsiHKCI	CYCGRG	1 cut(s)	1964
BsiSI	CCGG	5 cut(s)	826, 1647, 1781, 2075, 2222
BslFI	GGGAC	4 cut(s)	144, 313, 1448, 1519
BslI	CCNNNNNNNGG	1 cut(s)	2074
BsmAI	GTCTC	4 cut(s)	367, 751, 1023, 1834
BsmBI	CGTCTC	1 cut(s)	1834
BsmFI	GGGAC	4 cut(s)	144, 313, 1448, 1519
BsmI	GAATGC	5 cut(s)	111, 248, 519, 1084, 2015
BsnI	GGCC	6 cut(s)	303, 356, 1144, 1548, 1905, 2134
Bso31I	GGTCTC	2 cut(s)	367, 751
BsoBI	CYCGRG	1 cut(s)	1964
Bsp119I	TTCGAA	3 cut(s)	511, 2091, 2215
Bsp1286I	GDGCHC	3 cut(s)	1461, 1490, 2023
Bsp13I	TCCGGA	1 cut(s)	825
Bsp143I	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
Bsp68I	TCGCGA	1 cut(s)	679
BspACI	CCGC	9 cut(s)	375, 789, 869, 1650, 1684, 1922, 2031, 2162, 2243
BspANI	GGCC	6 cut(s)	303, 356, 1144, 1548, 1905, 2134
BspCNI	CTCAG	4 cut(s)	747, 1827, 1906, 2007
BspDI	ATCGAT	1 cut(s)	2138
BspEI	TCCGGA	1 cut(s)	825
BspFNI	CGCG	1 cut(s)	679
BspHI	TCATGA	2 cut(s)	67, 943
BspLI	GGNNCC	3 cut(s)	938, 1298, 1478
BspMAI	CTGCAG	4 cut(s)	240, 1006, 2123, 2193
BspOI	GCTAGC	1 cut(s)	1095
BspPI	GGATC	3 cut(s)	745, 784, 2070
BspT104I	TTCGAA	3 cut(s)	511, 2091, 2215
BspT107I	GGYRCC	1 cut(s)	1476
BspTNI	GGTCTC	2 cut(s)	367, 751
BsrDI	GCAATG	5 cut(s)	336, 1089, 1412, 2106, 2142
BsrFI	RCCGGY	1 cut(s)	2221
BsrI	ACTGG	2 cut(s)	1044, 1953
BssAI	RCCGGY	1 cut(s)	2221
BssECI	CCNNGG	4 cut(s)	357, 522, 984, 1858
BssMI	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
BssNAI	GTATAC	1 cut(s)	564
BssT1I	CCWWGG	1 cut(s)	357
Bst1107I	GTATAC	1 cut(s)	564
Bst2UI	CCWGG	2 cut(s)	524, 977
Bst4CI	ACNGT	4 cut(s)	608, 1317, 1510, 1948
Bst6I	CTCTTC	1 cut(s)	1008
BstBI	TTCGAA	3 cut(s)	511, 2091, 2215
BstC8I	GCNNGC	2 cut(s)	1093, 1351
BstDEI	CTNAG	5 cut(s)	755, 1071, 1814, 1893, 2015
BstDSI	CCRYGG	1 cut(s)	984
BstF5I	GGATG	6 cut(s)	480, 624, 834, 1930, 2089, 2251
BstFNI	CGCG	1 cut(s)	679
BstH2I	RGCGCY	1 cut(s)	773
BstHHI	GCGC	2 cut(s)	371, 772
BstKTI	GATC	7 cut(s)	67, 753, 779, 1338, 1847, 2065, 2142
BstMAI	GTCTC	4 cut(s)	367, 751, 1023, 1834
BstMBI	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
BstMWI	GCNNNNNNNGC	7 cut(s)	547, 818, 1088, 1097, 1355, 2027, 2153
BstNI	CCWGG	2 cut(s)	524, 977
BstNSI	RCATGY	2 cut(s)	509, 2074
BstSCI	CCNGG	4 cut(s)	522, 975, 1645, 2074
BstSFI	CTRYAG	7 cut(s)	180, 236, 813, 1002, 1836, 2119, 2189
BstSLI	GKGCMC	1 cut(s)	2023
BstUI	CGCG	1 cut(s)	679
BstV1I	GCAGC	7 cut(s)	191, 830, 988, 1333, 1947, 2133, 2203
BstV2I	GAAGAC	1 cut(s)	1989
BstX2I	RGATCY	3 cut(s)	776, 1844, 2062
BstXI	CCANNNNNNTGG	1 cut(s)	470
BstYI	RGATCY	3 cut(s)	776, 1844, 2062
BstZ17I	GTATAC	1 cut(s)	564
Bsu15I	ATCGAT	1 cut(s)	2138
BsuRI	GGCC	6 cut(s)	303, 356, 1144, 1548, 1905, 2134
BsuTUI	ATCGAT	1 cut(s)	2138
BtgI	CCRYGG	1 cut(s)	984
BtsCI	GGATG	6 cut(s)	480, 624, 834, 1930, 2089, 2251
BtsI	GCAGTG	1 cut(s)	67
BtsIMutI	CAGTG	2 cut(s)	67, 1906
BtuMI	TCGCGA	1 cut(s)	679
Cac8I	GCNNGC	2 cut(s)	1093, 1351
CciI	TCATGA	2 cut(s)	67, 943
CfoI	GCGC	2 cut(s)	371, 772
Cfr10I	RCCGGY	1 cut(s)	2221
Cfr13I	GGNCC	2 cut(s)	1142, 1547
ClaI	ATCGAT	1 cut(s)	2138
CsiI	ACCWGGT	1 cut(s)	975
Csp6I	GTAC	6 cut(s)	257, 672, 864, 1007, 1477, 1597
CspAI	ACCGGT	1 cut(s)	2221
CspCI	CAANNNNNGTGG	2 cut(s)	1553, 1588
CviQI	GTAC	6 cut(s)	257, 672, 864, 1007, 1477, 1597
DdeI	CTNAG	5 cut(s)	755, 1071, 1814, 1893, 2015
DpnI	GATC	7 cut(s)	66, 752, 778, 1337, 1846, 2064, 2141
DpnII	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
EaeI	YGGCCR	2 cut(s)	301, 2132
Eam1104I	CTCTTC	1 cut(s)	1008
EarI	CTCTTC	1 cut(s)	1008
EciI	GGCGGA	2 cut(s)	1665, 2258
Eco130I	CCWWGG	1 cut(s)	357
Eco31I	GGTCTC	2 cut(s)	367, 751
Eco47III	AGCGCT	1 cut(s)	771
Eco57I	CTGAAG	2 cut(s)	824, 1705
Eco88I	CYCGRG	1 cut(s)	1964
EcoRII	CCWGG	2 cut(s)	522, 975
EcoT14I	CCWWGG	1 cut(s)	357
EcoT22I	ATGCAT	1 cut(s)	1152
ErhI	CCWWGG	1 cut(s)	357
Esp3I	CGTCTC	1 cut(s)	1834
FaqI	GGGAC	4 cut(s)	144, 313, 1448, 1519
FauNDI	CATATG	1 cut(s)	1467
FbaI	TGATCA	2 cut(s)	64, 1335
FblI	GTMKAC	2 cut(s)	563, 1835
Fnu4HI	GCNGC	9 cut(s)	205, 819, 1002, 1347, 1685, 1961, 2031, 2122, 2192
FokI	GGATG	6 cut(s)	487, 631, 841, 1937, 2096, 2258
Fsp4HI	GCNGC	9 cut(s)	205, 819, 1002, 1347, 1685, 1961, 2031, 2122, 2192
FspBI	CTAG	2 cut(s)	1092, 1233
FspI	TGCGCA	1 cut(s)	370
GlaI	GCGC	2 cut(s)	370, 771
GluI	GCNGC	9 cut(s)	205, 819, 1002, 1347, 1685, 1961, 2031, 2122, 2192
GsaI	CCCAGC	2 cut(s)	776, 2198
GsuI	CTGGAG	2 cut(s)	948, 971
HaeII	RGCGCY	1 cut(s)	773
HaeIII	GGCC	6 cut(s)	303, 356, 1144, 1548, 1905, 2134
HapII	CCGG	5 cut(s)	826, 1647, 1781, 2075, 2222
HhaI	GCGC	2 cut(s)	371, 772
Hin6I	GCGC	2 cut(s)	369, 770
HinP1I	GCGC	2 cut(s)	369, 770
HinfI	GANTC	9 cut(s)	406, 764, 794, 803, 1016, 1126, 1777, 2106, 2212
HpaII	CCGG	5 cut(s)	826, 1647, 1781, 2075, 2222
HphI	GGTGA	5 cut(s)	325, 803, 812, 1055, 1438
Hpy166II	GTNNAC	6 cut(s)	564, 866, 1046, 1216, 1836, 1939
Hpy188I	TCNGA	5 cut(s)	915, 1116, 1531, 1746, 1862
Hpy8I	GTNNAC	6 cut(s)	564, 866, 1046, 1216, 1836, 1939
HpyAV	CCTTC	3 cut(s)	484, 1111, 1287
HpyCH4III	ACNGT	4 cut(s)	608, 1317, 1510, 1948
HpyCH4IV	ACGT	3 cut(s)	293, 311, 590
HpyF10VI	GCNNNNNNNGC	7 cut(s)	547, 818, 1088, 1097, 1355, 2027, 2153
HpyF3I	CTNAG	5 cut(s)	755, 1071, 1814, 1893, 2015
HpySE526I	ACGT	3 cut(s)	293, 311, 590
HspAI	GCGC	2 cut(s)	369, 770
Kpn2I	TCCGGA	1 cut(s)	825
KpnI	GGTACC	1 cut(s)	1480
Ksp22I	TGATCA	2 cut(s)	64, 1335
Kzo9I	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
LmnI	GCTCC	4 cut(s)	108, 936, 1873, 2167
Lsp1109I	GCAGC	7 cut(s)	191, 830, 988, 1333, 1947, 2133, 2203
LweI	GCATC	4 cut(s)	312, 609, 819, 1740
MabI	ACCWGGT	1 cut(s)	975
MaeI	CTAG	2 cut(s)	1092, 1233
MaeII	ACGT	3 cut(s)	293, 311, 590
MaeIII	GTNAC	4 cut(s)	43, 305, 1948, 1984
MalI	GATC	7 cut(s)	66, 752, 778, 1337, 1846, 2064, 2141
MboI	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
MboII	GAAGA	6 cut(s)	493, 902, 1025, 1087, 1747, 1989
MfeI	CAATTG	1 cut(s)	18
MflI	RGATCY	3 cut(s)	776, 1844, 2062
MhlI	GDGCHC	3 cut(s)	1461, 1490, 2023
MlsI	TGGCCA	1 cut(s)	2134
MluNI	TGGCCA	1 cut(s)	2134
MlyI	GAGTC	3 cut(s)	803, 1025, 1135
MmeI	TCCRAC	4 cut(s)	340, 702, 914, 1911
Mox20I	TGGCCA	1 cut(s)	2134
Mph1103I	ATGCAT	1 cut(s)	1152
MroI	TCCGGA	1 cut(s)	825
MscI	TGGCCA	1 cut(s)	2134
MseI	TTAA	4 cut(s)	5, 389, 705, 1190
MslI	CAYNNNNRTG	4 cut(s)	606, 1211, 1674, 1692
Msp20I	TGGCCA	1 cut(s)	2134
MspA1I	CMGCKG	2 cut(s)	204, 2194
MspI	CCGG	5 cut(s)	826, 1647, 1781, 2075, 2222
MspR9I	CCNGG	4 cut(s)	524, 977, 1647, 2076
MunI	CAATTG	1 cut(s)	18
Mva1269I	GAATGC	5 cut(s)	111, 248, 519, 1084, 2015
MvaI	CCWGG	2 cut(s)	524, 977
MvnI	CGCG	1 cut(s)	679
MwoI	GCNNNNNNNGC	7 cut(s)	547, 818, 1088, 1097, 1355, 2027, 2153
NciI	CCSGG	2 cut(s)	1647, 2076
NdeI	CATATG	1 cut(s)	1467
NdeII	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
NheI	GCTAGC	1 cut(s)	1091
NlaIV	GGNNCC	3 cut(s)	938, 1298, 1478
NmuCI	GTSAC	1 cut(s)	1948
NruI	TCGCGA	1 cut(s)	679
NsbI	TGCGCA	1 cut(s)	370
NsiI	ATGCAT	1 cut(s)	1152
NspI	RCATGY	2 cut(s)	509, 2074
NspV	TTCGAA	3 cut(s)	511, 2091, 2215
PaeR7I	CTCGAG	1 cut(s)	1964
PagI	TCATGA	2 cut(s)	67, 943
PcsI	WCGNNNNNNNCGW	1 cut(s)	215
PctI	GAATGC	5 cut(s)	111, 248, 519, 1084, 2015
PfeI	GAWTC	6 cut(s)	406, 764, 803, 1777, 2106, 2212
PflFI	GACNNNGTC	1 cut(s)	977
PinAI	ACCGGT	1 cut(s)	2221
PkrI	GCNGC	9 cut(s)	206, 820, 1003, 1348, 1686, 1962, 2032, 2123, 2193
PleI	GAGTC	3 cut(s)	802, 1024, 1134
PpsI	GAGTC	3 cut(s)	802, 1024, 1134
PshBI	ATTAAT	1 cut(s)	705
Psp6I	CCWGG	2 cut(s)	522, 975
PspFI	CCCAGC	2 cut(s)	772, 2194
PspGI	CCWGG	2 cut(s)	522, 975
PspN4I	GGNNCC	3 cut(s)	938, 1298, 1478
PspPI	GGNCC	2 cut(s)	1142, 1547
PstI	CTGCAG	4 cut(s)	240, 1006, 2123, 2193
PsuI	RGATCY	3 cut(s)	776, 1844, 2062
PsyI	GACNNNGTC	1 cut(s)	977
PvuII	CAGCTG	2 cut(s)	204, 2194
RruI	TCGCGA	1 cut(s)	679
RsaI	GTAC	6 cut(s)	258, 673, 865, 1008, 1478, 1598
RsaNI	GTAC	6 cut(s)	257, 672, 864, 1007, 1477, 1597
RseI	CAYNNNNRTG	4 cut(s)	606, 1211, 1674, 1692
SaqAI	TTAA	4 cut(s)	5, 389, 705, 1190
SatI	GCNGC	9 cut(s)	205, 819, 1002, 1347, 1685, 1961, 2031, 2122, 2192
Sau3AI	GATC	7 cut(s)	64, 750, 776, 1335, 1844, 2062, 2139
Sau96I	GGNCC	2 cut(s)	1142, 1547
ScaI	AGTACT	2 cut(s)	673, 1008
SchI	GAGTC	3 cut(s)	803, 1025, 1135
ScrFI	CCNGG	4 cut(s)	524, 977, 1647, 2076
SduI	GDGCHC	3 cut(s)	1461, 1490, 2023
SexAI	ACCWGGT	1 cut(s)	975
SfaNI	GCATC	4 cut(s)	312, 609, 819, 1740
SfcI	CTRYAG	7 cut(s)	180, 236, 813, 1002, 1836, 2119, 2189
Sfr274I	CTCGAG	1 cut(s)	1964
SfuI	TTCGAA	3 cut(s)	511, 2091, 2215
SlaI	CTCGAG	1 cut(s)	1964
SmiMI	CAYNNNNRTG	4 cut(s)	606, 1211, 1674, 1692
SmlI	CTYRAG	3 cut(s)	436, 1714, 1964
SmoI	CTYRAG	3 cut(s)	436, 1714, 1964
SpeI	ACTAGT	1 cut(s)	1232
SsiI	CCGC	9 cut(s)	375, 789, 869, 1650, 1684, 1922, 2031, 2162, 2243
SspMI	CTAG	2 cut(s)	1092, 1233
StyD4I	CCNGG	4 cut(s)	522, 975, 1645, 2074
StyI	CCWWGG	1 cut(s)	357
TaaI	ACNGT	4 cut(s)	608, 1317, 1510, 1948
TaiI	ACGT	3 cut(s)	296, 314, 593
TaqI	TCGA	9 cut(s)	209, 511, 1054, 1172, 1965, 2091, 2138, 2215, 2257
TatI	WGTACW	4 cut(s)	256, 671, 1006, 1596
TauI	GCSGC	2 cut(s)	1687, 2033
TfiI	GAWTC	6 cut(s)	406, 764, 803, 1777, 2106, 2212
Tru1I	TTAA	4 cut(s)	5, 389, 705, 1190
Tru9I	TTAA	4 cut(s)	5, 389, 705, 1190
TscAI	CASTG	2 cut(s)	67, 1906
TseFI	GTSAC	1 cut(s)	1948
TseI	GCWGC	7 cut(s)	204, 818, 1001, 1346, 1960, 2121, 2191
Tsp45I	GTSAC	1 cut(s)	1948
TspDTI	ATGAA	8 cut(s)	241, 494, 659, 1325, 1713, 1790, 2216, 2262
TspGWI	ACGGA	3 cut(s)	207, 1305, 2220
TspRI	CASTG	2 cut(s)	67, 1906
Tth111I	GACNNNGTC	1 cut(s)	977
VspI	ATTAAT	1 cut(s)	705
XapI	RAATTY	6 cut(s)	211, 850, 922, 1186, 1616, 1882
XceI	RCATGY	2 cut(s)	509, 2074
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1780
XhoI	CTCGAG	1 cut(s)	1964
XmiI	GTMKAC	2 cut(s)	563, 1835
XspI	CTAG	2 cut(s)	1092, 1233
ZrmI	AGTACT	2 cut(s)	673, 1008
Zsp2I	ATGCAT	1 cut(s)	1152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.