Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0005257

Physical Location & Seq

Genomic Coordinates Forward (+)

23351132 .. 23354155

Gene Structure 3024 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g22480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGTTCAGAGCATTAGCAATTGGATTGTTGGTTTTGTGTTTCGTTACATTAATTTCGGGCAGTGATCATGACTTTGCACATTGCAATTGTGACGAGGAGGGTTTTTGGAGCATTCCAAACATTCTGGAGTGCCAAAGAGTGAGTGATTTCTTGATTGCAATAGCATATTTTTCAATCCCGATAGAACTCCTTTACTTTGTCAGCTGCTCGAACGTTCCATTCAAATGGGTCCTCCTGCAGTTTATTGCCTTCATAGTTCTCTGTGGGTTGACCCATTTGCTGAATGCGTGGACGTATTATGGCCGCTACACGTTCCAGTTGATGCTGTCCCTCACCATTGCCAAATTCCTCACGGCCTTGGTCTCTTGCGCAACCGCAATAACCCTTTTAACTCTGTTCCCTATGATTCTCAAAGTAAAAGTGAGAGAATTGTTCTTGAGGCAAAATGTGATGGAATTAGACCAAGAGGTTGGGATGATGAAGATACAGAAGGAAGCGAGTTGGCATGTTCGAATGCTGACCCAGGAAATTAGAAAGTCGCTTGACAAACACACCATATTGTATACAACTCTGGTTGAGCTTTCAAAGACACTAGACTTGCATAATTGTGCAGTTTGGATGCCGAATGAGAAAAGAGGAGAGATGAACCTGACCCATGAGTTGAAAACAAGTTCTTCGCGACAGTATCGCCGTTCTATCCCAATTAATGACCCAGATGTGTTGGAGATAAGAGAGAGCGAGAGGGTAATGATCCTGAGGCCTGATTCGGCATTGGGGTCTGCAAGTAGCGGTGAGTCTAGTGAATCAGGTGCTGTAGCAGCAATCCGGATGCCAATGCTTCGGGTTTCAAATTTCAAAGGCGGTACGCCTCAGTTGGTTGATACACATTATGCTATATTGGTTTTGGTTCTTCCGGTTGCAGATTCTGGAGGTTGGAGCCATCATGAGCTGGAGATAGTGGAAGTTGTGGCTGACCAAGTTGCCGTGGCTCTATCCCATGCTGCAGTACTTGAAGAGTCTCAACTAATGAGGGAGAAACTGGCTGAACAAAATCGAGCGTTGCAACAGGCTAAGAAGAATGCAATGATGGCCAGCCAAGCAAGGCACTCGTTTCAGAAGGTAATGAGTCATGGAATGAGAAGGCCAATGCATATGATTTTGGGTTTGCTTTCAATGTTCCAAGAGAATTTAAGTTTCAAACAGAGCATTATTGTGGACACAATGGAGAAAACTAGTTATGTTATCTGCACTTTGATAAATGATGTGATGGAGATGTCGGCAAAAGATAATGGAAGGTTCCCGTTGGAGATGAGGCCGTTTGAACTACATTCTATGATCAAGGAAGCTGCTTGCCTTGCCAAATGCTTGTGTATGTATAAGGGCTTTAGCTTTGAAATTGATGTTCAAAGCTCATTGCCTAATCAGGTGATAGGAGATGAGAAAAGGACTTTCCAAGTACTCTTGCATATGATTGGGTACCTATTGAGTACCTATAATGGTGGGGGGACTGTCATCTTTCGGGCTATTTCTGTGAGTGGTACTGATGGCCCTGATGATAGATTGCAAGGAATGTGGAGAATGGGTATTCCAGATGAGTACATTTGTATAAAGTTTGAATTTGAGATTAACGAGGCAAATTCTTATCCCGGCGGATTAGTAGCATCGACGCACGATGCTCGTAGGCAGCACAACAATGATGCAATCAAGAAGGGCCTGAGCTTCAGCATTTGTAAGAAGATTGTCCAGATGATGCAAGGTAATATCTGGATATCCATGAACCCAGTGGAATTTGCAGAAAGCATGACACTTGTTCTCAGGTTTCAAATCCTGTCATCTATGGGAAGAACTATATGTCCCCTGGGAAATACTTTGGAGCAACCAAATTCCAACTCGCAGTTCAAAGGCCTTCGGGTTATGGTAGCGGATGATGATAACATAAACAGGACTGTGACCATTAAGCTCCTCGAGAAACTTGGCTGTCAAGTTACCGCTGTATCTTCCGGGTTTGAATGCCTGAGTGCTCTTACTGCCGCCGAAAACTCTTTTCAAATTGTTGTCTTGGATCTCCACATGCCGGAAATGGATGGGTTCGAAGTAGCATTGAGAATTAGGAAGTTCCGCAGCCATAGTTGGCCATTGATCATAGCCCTCACAGCGAGTGCGGAGGAGCATATATGGGAGAGATGTCTGCAGCTGGGAATGAACGGATTGATTCGAAAACCGGTTCTTTTGCAAGGGATGGCGGATGAACTTCGTCGAGTTCTACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

757

Amino Acids

85.25

Weight (kDa)

6.42

Isoelectric Point (pI)

41.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	40 - 139	1.7e-41	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	183 - 331	4.8e-11	GAF domain
HisKA	PF00512	368 - 431	1.5e-06	His Kinase A (phospho-acceptor) domain
Response_reg	PF00072	638 - 746	3.3e-20	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04580 AT3G04580
fragaria_vesca	FvH4_3g14281 FvH4_5g22480 FvH4_6g02340
malus_domestica	MD00G1114300.v1.1 MD12G1245100.v1.1
prunus_persica	Prupe.6G348000_v2.0.a1
pyrus_communis	pycom04g20230 pycom04g20240 pycom12g22480
rosa_chinensis	RchiOBHm_Chr3g0450101 RchiOBHm_Chr6g0274471
rosa_laevigata	RLG00000008654 RLG00000025756
rosa_multiflora	Rmu_sc0018882.1_g000003 Rmu_sc0035048.1_g000001 Rmu_sc0035049.1_g000001
rosa_roxburghii	Rroxscaffold_2G00117790 Rroxscaffold_3G00276360 Rroxscaffold_7G00200060
rosa_rugosa	Rorug02G0625400 Rorug02G0625500 Rorug04G0237900
rosa_samantha	Rh3AG024700 Rh3BG025300 Rh3CG024200 Rh3DG025300
rosa_wichuraiana	Rw3G001970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	370
Acc65I	GGTACC	1 cut(s)	1476
AccB1I	GGYRCC	1 cut(s)	1476
AccI	GTMKAC	1 cut(s)	563
AccII	CGCG	1 cut(s)	679
AccIII	TCCGGA	1 cut(s)	825
AclI	AACGTT	1 cut(s)	213
AclWI	GGATC	2 cut(s)	745, 2070
AcoI	YGGCCR	3 cut(s)	301, 1089, 2132
AcsI	RAATTY	7 cut(s)	344, 850, 1186, 1616, 1636, 1787, 1882
AcuI	CTGAAG	1 cut(s)	1705
AfaI	GTAC	7 cut(s)	865, 1008, 1458, 1478, 1489, 1540, 1598
AfiI	CCNNNNNNNGG	1 cut(s)	2074
AflIII	ACRYGT	1 cut(s)	309
AgeI	ACCGGT	1 cut(s)	2221
AhlI	ACTAGT	1 cut(s)	1232
AjnI	CCWGG	2 cut(s)	522, 1857
AjuI	GAANNNNNNNTTGG	2 cut(s)	1880, 1912
AloI	GAACNNNNNNTCC	2 cut(s)	1837, 1869
AluBI	AGCT	9 cut(s)	204, 580, 949, 1346, 1389, 1410, 1719, 1960, 2194
AluI	AGCT	9 cut(s)	204, 580, 949, 1346, 1389, 1410, 1719, 1960, 2194
Alw21I	GWGCWC	1 cut(s)	2023
Alw26I	GTCTC	2 cut(s)	367, 1023
AlwI	GGATC	2 cut(s)	745, 2070
AlwNI	CAGNNNCTG	2 cut(s)	812, 926
Ama87I	CYCGRG	1 cut(s)	1964
Aor13HI	TCCGGA	1 cut(s)	825
ApeKI	GCWGC	7 cut(s)	204, 818, 1001, 1346, 1684, 2121, 2191
ApoI	RAATTY	7 cut(s)	344, 850, 1186, 1616, 1636, 1787, 1882
AseI	ATTAAT	2 cut(s)	50, 705
AsiGI	ACCGGT	1 cut(s)	2221
Asp718I	GGTACC	1 cut(s)	1476
AspLEI	GCGC	1 cut(s)	371
AspS9I	GGNCC	3 cut(s)	229, 1547, 1711
AsuC2I	CCSGG	2 cut(s)	1647, 2002
AsuHPI	GGTGA	3 cut(s)	325, 803, 1438
AsuII	TTCGAA	3 cut(s)	511, 2091, 2215
AvaI	CYCGRG	1 cut(s)	1964
AvaII	GGWCC	1 cut(s)	229
AxyI	CCTNAGG	1 cut(s)	755
BaeI	ACNNNNGTAYC	2 cut(s)	1977, 2010
BalI	TGGCCA	2 cut(s)	1091, 2134
BanI	GGYRCC	1 cut(s)	1476
Bbv12I	GWGCWC	1 cut(s)	2023
BbvI	GCAGC	7 cut(s)	191, 830, 988, 1333, 1696, 2133, 2203
BccI	CCATC	7 cut(s)	445, 948, 1081, 1261, 1538, 2078, 2233
BceAI	ACGGC	4 cut(s)	369, 675, 968, 1300
BcgI	CGANNNNNNTGC	4 cut(s)	1089, 1123, 1658, 1692
BciT130I	CCWGG	2 cut(s)	524, 1859
BclI	TGATCA	3 cut(s)	64, 1335, 2139
BcnI	CCSGG	2 cut(s)	1647, 2002
BcoDI	GTCTC	2 cut(s)	367, 1023
BcuI	ACTAGT	1 cut(s)	1232
BfaI	CTAG	3 cut(s)	593, 798, 1233
BfmI	CTRYAG	4 cut(s)	236, 813, 1002, 2189
BisI	GCNGC	9 cut(s)	205, 304, 819, 1002, 1347, 1685, 2031, 2122, 2192
BlsI	GCNGC	9 cut(s)	206, 305, 820, 1003, 1348, 1686, 2032, 2123, 2193
BmcAI	AGTACT	2 cut(s)	1008, 1458
Bme1390I	CCNGG	4 cut(s)	524, 1647, 1859, 2002
Bme18I	GGWCC	1 cut(s)	229
BmeT110I	CYCGRG	1 cut(s)	1964
BmgT120I	GGNCC	3 cut(s)	229, 1547, 1711
BmiI	GGNNCC	4 cut(s)	230, 938, 1298, 1478
BmrFI	CCNGG	4 cut(s)	524, 1647, 1859, 2002
BmrI	ACTGGG	1 cut(s)	1775
BmsI	GCATC	7 cut(s)	312, 609, 819, 1663, 1670, 1687, 1740
BmuI	ACTGGG	1 cut(s)	1775
BpmI	CTGGAG	3 cut(s)	146, 948, 971
Bpu10I	CCTNAGC	1 cut(s)	1715
Bpu14I	TTCGAA	3 cut(s)	511, 2091, 2215
BpuEI	CTTGAG	1 cut(s)	457
BpuMI	CCSGG	2 cut(s)	1647, 2002
BsaI	GGTCTC	1 cut(s)	367
BsaJI	CCNNGG	5 cut(s)	357, 522, 984, 1857, 1858
BsaWI	WCCGGW	3 cut(s)	825, 913, 2221
BsaXI	ACNNNNNCTCC	2 cut(s)	1567, 1597
Bsc4I	CCNNNNNNNGG	1 cut(s)	2074
Bse118I	RCCGGY	1 cut(s)	2221
Bse1I	ACTGG	3 cut(s)	316, 1044, 1781
Bse21I	CCTNAGG	1 cut(s)	755
Bse3DI	GCAATG	4 cut(s)	79, 336, 1089, 1412
BseAI	TCCGGA	1 cut(s)	825
BseBI	CCWGG	2 cut(s)	524, 1859
BseDI	CCNNGG	5 cut(s)	357, 522, 984, 1857, 1858
BseGI	GGATG	7 cut(s)	480, 624, 834, 1930, 2089, 2244, 2251
BseLI	CCNNNNNNNGG	1 cut(s)	2074
BseMI	GCAATG	4 cut(s)	79, 336, 1089, 1412
BseMII	CTCAG	5 cut(s)	746, 884, 1706, 1828, 2006
BseNI	ACTGG	3 cut(s)	316, 1044, 1781
BseRI	GAGGAG	4 cut(s)	110, 651, 1952, 2180
BseXI	GCAGC	7 cut(s)	191, 830, 988, 1333, 1696, 2133, 2203
BseYI	CCCAGC	1 cut(s)	2194
BsgI	GTGCAG	2 cut(s)	630, 1231
Bsh1236I	CGCG	1 cut(s)	679
BshNI	GGYRCC	1 cut(s)	1476
BshTI	ACCGGT	1 cut(s)	2221
BsiHKAI	GWGCWC	1 cut(s)	2023
BsiHKCI	CYCGRG	1 cut(s)	1964
BsiSI	CCGG	6 cut(s)	826, 914, 1647, 2001, 2075, 2222
BslFI	GGGAC	3 cut(s)	313, 1519, 1839
BslI	CCNNNNNNNGG	1 cut(s)	2074
BsmAI	GTCTC	2 cut(s)	367, 1023
BsmFI	GGGAC	3 cut(s)	313, 1519, 1839
BsmI	GAATGC	5 cut(s)	111, 289, 519, 1084, 2015
Bso31I	GGTCTC	1 cut(s)	367
BsoBI	CYCGRG	1 cut(s)	1964
Bsp119I	TTCGAA	3 cut(s)	511, 2091, 2215
Bsp1286I	GDGCHC	1 cut(s)	2023
Bsp13I	TCCGGA	1 cut(s)	825
Bsp143I	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
Bsp68I	TCGCGA	1 cut(s)	679
BspCNI	CTCAG	5 cut(s)	747, 883, 1707, 1827, 2007
BspEI	TCCGGA	1 cut(s)	825
BspFNI	CGCG	1 cut(s)	679
BspHI	TCATGA	2 cut(s)	67, 943
BspLI	GGNNCC	4 cut(s)	230, 938, 1298, 1478
BspMAI	CTGCAG	3 cut(s)	240, 1006, 2193
BspPI	GGATC	2 cut(s)	745, 2070
BspT104I	TTCGAA	3 cut(s)	511, 2091, 2215
BspT107I	GGYRCC	1 cut(s)	1476
BspTNI	GGTCTC	1 cut(s)	367
BsrDI	GCAATG	4 cut(s)	79, 336, 1089, 1412
BsrFI	RCCGGY	1 cut(s)	2221
BsrI	ACTGG	3 cut(s)	316, 1044, 1781
BssAI	RCCGGY	1 cut(s)	2221
BssECI	CCNNGG	5 cut(s)	357, 522, 984, 1857, 1858
BssMI	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
BssNAI	GTATAC	1 cut(s)	564
BssT1I	CCWWGG	1 cut(s)	357
Bst1107I	GTATAC	1 cut(s)	564
Bst2UI	CCWGG	2 cut(s)	524, 1859
Bst4CI	ACNGT	3 cut(s)	684, 1510, 1948
Bst6I	CTCTTC	1 cut(s)	1008
BstBI	TTCGAA	3 cut(s)	511, 2091, 2215
BstC8I	GCNNGC	2 cut(s)	1093, 1351
BstDEI	CTNAG	6 cut(s)	755, 870, 1071, 1715, 1814, 2015
BstDSI	CCRYGG	1 cut(s)	984
BstF5I	GGATG	7 cut(s)	480, 624, 834, 1930, 2089, 2244, 2251
BstFNI	CGCG	1 cut(s)	679
BstHHI	GCGC	1 cut(s)	371
BstKTI	GATC	5 cut(s)	67, 753, 1338, 2065, 2142
BstMAI	GTCTC	2 cut(s)	367, 1023
BstMBI	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
BstMWI	GCNNNNNNNGC	6 cut(s)	818, 1088, 1097, 1355, 2027, 2153
BstNI	CCWGG	2 cut(s)	524, 1859
BstNSI	RCATGY	2 cut(s)	509, 2074
BstSCI	CCNGG	4 cut(s)	522, 1645, 1857, 2000
BstSFI	CTRYAG	4 cut(s)	236, 813, 1002, 2189
BstUI	CGCG	1 cut(s)	679
BstV1I	GCAGC	7 cut(s)	191, 830, 988, 1333, 1696, 2133, 2203
BstX2I	RGATCY	1 cut(s)	2062
BstXI	CCANNNNNNTGG	2 cut(s)	225, 470
BstYI	RGATCY	1 cut(s)	2062
BstZ17I	GTATAC	1 cut(s)	564
Bsu36I	CCTNAGG	1 cut(s)	755
BtgI	CCRYGG	1 cut(s)	984
BtsCI	GGATG	7 cut(s)	480, 624, 834, 1930, 2089, 2244, 2251
BtsI	GCAGTG	1 cut(s)	67
BtsIMutI	CAGTG	2 cut(s)	67, 1788
BtuMI	TCGCGA	1 cut(s)	679
Cac8I	GCNNGC	2 cut(s)	1093, 1351
CaiI	CAGNNNCTG	2 cut(s)	812, 926
CciI	TCATGA	2 cut(s)	67, 943
CfoI	GCGC	1 cut(s)	371
Cfr10I	RCCGGY	1 cut(s)	2221
Cfr13I	GGNCC	3 cut(s)	229, 1547, 1711
CseI	GACGC	1 cut(s)	1675
Csp6I	GTAC	7 cut(s)	864, 1007, 1457, 1477, 1488, 1539, 1597
CspAI	ACCGGT	1 cut(s)	2221
CspCI	CAANNNNNGTGG	2 cut(s)	1553, 1588
CviAII	CATG	9 cut(s)	68, 506, 656, 944, 998, 1130, 1774, 1801, 2071
CviQI	GTAC	7 cut(s)	864, 1007, 1457, 1477, 1488, 1539, 1597
DdeI	CTNAG	6 cut(s)	755, 870, 1071, 1715, 1814, 2015
DpnI	GATC	5 cut(s)	66, 752, 1337, 2064, 2141
DpnII	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
EaeI	YGGCCR	3 cut(s)	301, 1089, 2132
Eam1104I	CTCTTC	1 cut(s)	1008
EarI	CTCTTC	1 cut(s)	1008
EciI	GGCGGA	2 cut(s)	1665, 2258
Eco130I	CCWWGG	1 cut(s)	357
Eco147I	AGGCCT	2 cut(s)	760, 1905
Eco31I	GGTCTC	1 cut(s)	367
Eco32I	GATATC	1 cut(s)	1770
Eco47I	GGWCC	1 cut(s)	229
Eco57I	CTGAAG	1 cut(s)	1705
Eco81I	CCTNAGG	1 cut(s)	755
Eco88I	CYCGRG	1 cut(s)	1964
EcoO109I	RGGNCCY	2 cut(s)	229, 1711
EcoRII	CCWGG	2 cut(s)	522, 1857
EcoRV	GATATC	1 cut(s)	1770
EcoT14I	CCWWGG	1 cut(s)	357
EcoT22I	ATGCAT	1 cut(s)	1152
ErhI	CCWWGG	1 cut(s)	357
FaeI	CATG	9 cut(s)	71, 509, 659, 947, 1001, 1133, 1777, 1804, 2074
FaqI	GGGAC	3 cut(s)	313, 1519, 1839
FatI	CATG	9 cut(s)	67, 505, 655, 943, 997, 1129, 1773, 1800, 2070
FauNDI	CATATG	2 cut(s)	1152, 1467
FbaI	TGATCA	3 cut(s)	64, 1335, 2139
FblI	GTMKAC	1 cut(s)	563
Fnu4HI	GCNGC	9 cut(s)	205, 304, 819, 1002, 1347, 1685, 2031, 2122, 2192
FokI	GGATG	7 cut(s)	487, 631, 841, 1937, 2096, 2251, 2258
Fsp4HI	GCNGC	9 cut(s)	205, 304, 819, 1002, 1347, 1685, 2031, 2122, 2192
FspBI	CTAG	3 cut(s)	593, 798, 1233
FspI	TGCGCA	1 cut(s)	370
GlaI	GCGC	1 cut(s)	370
GluI	GCNGC	9 cut(s)	205, 304, 819, 1002, 1347, 1685, 2031, 2122, 2192
GsaI	CCCAGC	1 cut(s)	2198
GsuI	CTGGAG	3 cut(s)	146, 948, 971
HapII	CCGG	6 cut(s)	826, 914, 1647, 2001, 2075, 2222
HgaI	GACGC	1 cut(s)	1675
HhaI	GCGC	1 cut(s)	371
Hin1II	CATG	9 cut(s)	71, 509, 659, 947, 1001, 1133, 1777, 1804, 2074
Hin6I	GCGC	1 cut(s)	369
HinP1I	GCGC	1 cut(s)	369
HincII	GTYRAC	1 cut(s)	270
HindII	GTYRAC	1 cut(s)	270
HinfI	GANTC	8 cut(s)	406, 764, 794, 803, 923, 1016, 1126, 2212
HpaII	CCGG	6 cut(s)	826, 914, 1647, 2001, 2075, 2222
HphI	GGTGA	3 cut(s)	325, 803, 1438
Hpy166II	GTNNAC	4 cut(s)	270, 291, 564, 1216
Hpy188I	TCNGA	2 cut(s)	8, 1116
Hpy8I	GTNNAC	4 cut(s)	270, 291, 564, 1216
Hpy99I	CGWCG	2 cut(s)	1669, 2259
HpyAV	CCTTC	7 cut(s)	259, 484, 1111, 1134, 1287, 1702, 1916
HpyCH4III	ACNGT	3 cut(s)	684, 1510, 1948
HpyCH4IV	ACGT	3 cut(s)	213, 293, 311
HpyF10VI	GCNNNNNNNGC	6 cut(s)	818, 1088, 1097, 1355, 2027, 2153
HpyF3I	CTNAG	6 cut(s)	755, 870, 1071, 1715, 1814, 2015
HpySE526I	ACGT	3 cut(s)	213, 293, 311
Hsp92II	CATG	9 cut(s)	71, 509, 659, 947, 1001, 1133, 1777, 1804, 2074
HspAI	GCGC	1 cut(s)	369
Kpn2I	TCCGGA	1 cut(s)	825
KpnI	GGTACC	1 cut(s)	1480
Ksp22I	TGATCA	3 cut(s)	64, 1335, 2139
Kzo9I	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
LmnI	GCTCC	5 cut(s)	108, 936, 1873, 1965, 2167
Lsp1109I	GCAGC	7 cut(s)	191, 830, 988, 1333, 1696, 2133, 2203
LweI	GCATC	7 cut(s)	312, 609, 819, 1663, 1670, 1687, 1740
MaeI	CTAG	3 cut(s)	593, 798, 1233
MaeII	ACGT	3 cut(s)	213, 293, 311
MaeIII	GTNAC	4 cut(s)	43, 89, 1948, 1984
MalI	GATC	5 cut(s)	66, 752, 1337, 2064, 2141
MboI	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
MboII	GAAGA	8 cut(s)	493, 666, 902, 1025, 1087, 1747, 1854, 1989
MfeI	CAATTG	2 cut(s)	18, 85
MflI	RGATCY	1 cut(s)	2062
MhlI	GDGCHC	1 cut(s)	2023
MlsI	TGGCCA	2 cut(s)	1091, 2134
MluNI	TGGCCA	2 cut(s)	1091, 2134
MlyI	GAGTC	3 cut(s)	803, 1025, 1135
MmeI	TCCRAC	4 cut(s)	702, 914, 1284, 1911
Mox20I	TGGCCA	2 cut(s)	1091, 2134
Mph1103I	ATGCAT	1 cut(s)	1152
MroI	TCCGGA	1 cut(s)	825
MscI	TGGCCA	2 cut(s)	1091, 2134
MseI	TTAA	6 cut(s)	50, 389, 705, 1190, 1626, 1956
MslI	CAYNNNNRTG	4 cut(s)	223, 606, 1211, 1692
Msp20I	TGGCCA	2 cut(s)	1091, 2134
MspA1I	CMGCKG	3 cut(s)	204, 1991, 2194
MspI	CCGG	6 cut(s)	826, 914, 1647, 2001, 2075, 2222
MspR9I	CCNGG	4 cut(s)	524, 1647, 1859, 2002
MunI	CAATTG	2 cut(s)	18, 85
Mva1269I	GAATGC	5 cut(s)	111, 289, 519, 1084, 2015
MvaI	CCWGG	2 cut(s)	524, 1859
MvnI	CGCG	1 cut(s)	679
MwoI	GCNNNNNNNGC	6 cut(s)	818, 1088, 1097, 1355, 2027, 2153
NciI	CCSGG	2 cut(s)	1647, 2002
NdeI	CATATG	2 cut(s)	1152, 1467
NdeII	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
NlaIII	CATG	9 cut(s)	71, 509, 659, 947, 1001, 1133, 1777, 1804, 2074
NlaIV	GGNNCC	4 cut(s)	230, 938, 1298, 1478
NmuCI	GTSAC	2 cut(s)	89, 1948
NruI	TCGCGA	1 cut(s)	679
NsbI	TGCGCA	1 cut(s)	370
NsiI	ATGCAT	1 cut(s)	1152
NspI	RCATGY	2 cut(s)	509, 2074
NspV	TTCGAA	3 cut(s)	511, 2091, 2215
PaeR7I	CTCGAG	1 cut(s)	1964
PagI	TCATGA	2 cut(s)	67, 943
PasI	CCCWGGG	1 cut(s)	1858
PceI	AGGCCT	2 cut(s)	760, 1905
PctI	GAATGC	5 cut(s)	111, 289, 519, 1084, 2015
PfeI	GAWTC	5 cut(s)	406, 764, 803, 923, 2212
PinAI	ACCGGT	1 cut(s)	2221
PkrI	GCNGC	9 cut(s)	206, 305, 820, 1003, 1348, 1686, 2032, 2123, 2193
PleI	GAGTC	3 cut(s)	802, 1024, 1134
PpsI	GAGTC	3 cut(s)	802, 1024, 1134
PpuMI	RGGWCCY	1 cut(s)	229
PshBI	ATTAAT	2 cut(s)	50, 705
Psp1406I	AACGTT	1 cut(s)	213
Psp5II	RGGWCCY	1 cut(s)	229
Psp6I	CCWGG	2 cut(s)	522, 1857
PspFI	CCCAGC	1 cut(s)	2194
PspGI	CCWGG	2 cut(s)	522, 1857
PspN4I	GGNNCC	4 cut(s)	230, 938, 1298, 1478
PspPI	GGNCC	3 cut(s)	229, 1547, 1711
PspPPI	RGGWCCY	1 cut(s)	229
PstI	CTGCAG	3 cut(s)	240, 1006, 2193
PstNI	CAGNNNCTG	2 cut(s)	812, 926
PsuI	RGATCY	1 cut(s)	2062
PvuII	CAGCTG	2 cut(s)	204, 2194
RruI	TCGCGA	1 cut(s)	679
RsaI	GTAC	7 cut(s)	865, 1008, 1458, 1478, 1489, 1540, 1598
RsaNI	GTAC	7 cut(s)	864, 1007, 1457, 1477, 1488, 1539, 1597
RseI	CAYNNNNRTG	4 cut(s)	223, 606, 1211, 1692
SaqAI	TTAA	6 cut(s)	50, 389, 705, 1190, 1626, 1956
SatI	GCNGC	9 cut(s)	205, 304, 819, 1002, 1347, 1685, 2031, 2122, 2192
Sau3AI	GATC	5 cut(s)	64, 750, 1335, 2062, 2139
Sau96I	GGNCC	3 cut(s)	229, 1547, 1711
ScaI	AGTACT	2 cut(s)	1008, 1458
SchI	GAGTC	3 cut(s)	803, 1025, 1135
ScrFI	CCNGG	4 cut(s)	524, 1647, 1859, 2002
SduI	GDGCHC	1 cut(s)	2023
SfaNI	GCATC	7 cut(s)	312, 609, 819, 1663, 1670, 1687, 1740
SfcI	CTRYAG	4 cut(s)	236, 813, 1002, 2189
Sfr274I	CTCGAG	1 cut(s)	1964
SfuI	TTCGAA	3 cut(s)	511, 2091, 2215
SinI	GGWCC	1 cut(s)	229
SlaI	CTCGAG	1 cut(s)	1964
SmiMI	CAYNNNNRTG	4 cut(s)	223, 606, 1211, 1692
SmlI	CTYRAG	2 cut(s)	436, 1964
SmoI	CTYRAG	2 cut(s)	436, 1964
SpeI	ACTAGT	1 cut(s)	1232
SseBI	AGGCCT	2 cut(s)	760, 1905
SspMI	CTAG	3 cut(s)	593, 798, 1233
StuI	AGGCCT	2 cut(s)	760, 1905
StyD4I	CCNGG	4 cut(s)	522, 1645, 1857, 2000
StyI	CCWWGG	1 cut(s)	357
TaaI	ACNGT	3 cut(s)	684, 1510, 1948
TaiI	ACGT	3 cut(s)	216, 296, 314
TaqI	TCGA	8 cut(s)	209, 511, 1054, 1664, 1965, 2091, 2215, 2257
TatI	WGTACW	3 cut(s)	1006, 1456, 1596
TauI	GCSGC	2 cut(s)	306, 2033
TfiI	GAWTC	5 cut(s)	406, 764, 803, 923, 2212
Tru1I	TTAA	6 cut(s)	50, 389, 705, 1190, 1626, 1956
Tru9I	TTAA	6 cut(s)	50, 389, 705, 1190, 1626, 1956
TscAI	CASTG	2 cut(s)	67, 1788
TseFI	GTSAC	2 cut(s)	89, 1948
TseI	GCWGC	7 cut(s)	204, 818, 1001, 1346, 1684, 2121, 2191
Tsp45I	GTSAC	2 cut(s)	89, 1948
TspDTI	ATGAA	6 cut(s)	241, 494, 659, 1790, 2216, 2262
TspGWI	ACGGA	1 cut(s)	2220
TspRI	CASTG	2 cut(s)	67, 1788
VpaK11BI	GGWCC	1 cut(s)	229
VspI	ATTAAT	2 cut(s)	50, 705
XapI	RAATTY	7 cut(s)	344, 850, 1186, 1616, 1636, 1787, 1882
XceI	RCATGY	2 cut(s)	509, 2074
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1780
XhoI	CTCGAG	1 cut(s)	1964
XmiI	GTMKAC	1 cut(s)	563
XspI	CTAG	3 cut(s)	593, 798, 1233
ZrmI	AGTACT	2 cut(s)	1008, 1458
Zsp2I	ATGCAT	1 cut(s)	1152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.