AT3G05250

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008784
Physical Location & Seq
Forward (+)
1495572 .. 1497523
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05250.3

Sequence Viewer

Length: 837 bp
ATGGCGGCGAAGGAGATCGCCGGCGGGAGCGGTTCTATTGACGATGGATCCGATTTTGATCCATGTCCTATTTGCCTTGGACAATTCCTCCGTGAATCCTACCTTGATACCTGTTTCCACAAGTTTTGTTTCAATTGTATAAAACAATGGATCAAAGTTGTTTCAAGCAAGGCCTCTAAGCAACGTTCCTCAGTGACATGTCCTCTCTGTAAGACAGAGAATTTATCCATCATCCACAACTACGATGGATGTTCTTTTGAGCGGCATTATATCGACCCGAATATTCCAGATGGTTTTGTATTAACAAAAGAACAGAGATACAGGTTGCAGTGTTACTACACCGAATCAGGTTTCCTCGCCGATGTATTCGATGTTGCACGGTTTTGGAAGCTGCAAAAGTTTCTCCAGCCGAACCGGTGCCTTGAAGCCTGGTTGAGGAGGGAGCTTCAAGCTCTGATGCAGGAGGAAGATGTTGATATTGTTCTGCATCATTTGGTCGGTGTGATGGAATCCTTCTGCAAAAGAATCAAACAGAGACGCAAACAAGAAACAAGAAGCGCAGAGACAACAAATCAAGAACAATTCAAAGCAGTTGTCTCAGAAGCAGCACGTCCATTTGTAATGGCGAGAACAGACCGATTTGTGGATGAACTTGAACTCTTTCTGGCCGCGGGACTAAACTTGGAGGCTTATGATGCAATCTATAAGCAGGGTTTAGAGGGGAACAACCGAAGAGAGATCGGTGCAGCTAGTGAAGAAAGAGAAGAAGTGGAAGAACATAATGTAAGAACACGAGTGACTCCTTATTTGTTTATCTTCGAGGAAGACTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4

Protein Analysis

278

Amino Acids

32.22

Weight (kDa)

5.34

Isoelectric Point (pI)

55.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 417
AccBSI CCGCTC 2 cut(s) 30, 262
AccII CGCG 1 cut(s) 671
AciI CCGC 6 cut(s) 5, 24, 30, 262, 669, 671
AclI AACGTT 1 cut(s) 184
AclWI GGATC 4 cut(s) 42, 53, 55, 158
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 1 cut(s) 220
AfiI CCNNNNNNNGG 2 cut(s) 435, 643
AflIII ACRYGT 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 414
AgsI TTSAA 6 cut(s) 133, 165, 425, 449, 586, 656
AjiI CACGTC 1 cut(s) 611
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 4 cut(s) 391, 445, 452, 749
AluI AGCT 4 cut(s) 391, 445, 452, 749
Alw26I GTCTC 3 cut(s) 529, 557, 601
AlwI GGATC 4 cut(s) 42, 53, 55, 158
AoxI GGCC 2 cut(s) 171, 666
ApeKI GCWGC 3 cut(s) 391, 605, 746
ApoI RAATTY 1 cut(s) 220
ArsI GACNNNNNNTTYG 4 cut(s) 579, 611, 790, 822
AsiGI ACCGGT 1 cut(s) 414
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 1 cut(s) 560
BamHI GGATCC 1 cut(s) 47
BanI GGYRCC 1 cut(s) 417
BauI CACGAG 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 831
BbvI GCAGC 3 cut(s) 378, 617, 758
BccI CCATC 5 cut(s) 38, 236, 239, 284, 499
BciT130I CCWGG 1 cut(s) 430
BcoDI GTCTC 3 cut(s) 529, 557, 601
BfaI CTAG 1 cut(s) 750
BisI GCNGC 6 cut(s) 6, 263, 392, 606, 669, 747
BlsI GCNGC 6 cut(s) 7, 264, 393, 607, 670, 748
Bme1390I CCNGG 1 cut(s) 430
BmgBI CACGTC 1 cut(s) 611
BmiI GGNNCC 2 cut(s) 49, 419
BmrFI CCNGG 1 cut(s) 430
BmsI GCATC 3 cut(s) 447, 496, 685
BpiI GAAGAC 1 cut(s) 831
BplI GAGNNNNNCTC 1 cut(s) 812
BpmI CTGGAG 1 cut(s) 389
BsaBI GATNNNNATC 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 76, 669
BsaWI WCCGGW 1 cut(s) 414
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 2 cut(s) 435, 643
Bse118I RCCGGY 2 cut(s) 20, 414
Bse8I GATNNNNATC 1 cut(s) 57
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 2 cut(s) 76, 669
BseGI GGATG 3 cut(s) 231, 254, 652
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 2 cut(s) 435, 643
BseMII CTCAG 2 cut(s) 204, 612
BseRI GAGGAG 1 cut(s) 451
BseXI GCAGC 3 cut(s) 378, 617, 758
BsgI GTGCAG 1 cut(s) 765
Bsh1236I CGCG 1 cut(s) 671
BshFI GGCC 2 cut(s) 173, 668
BshNI GGYRCC 1 cut(s) 417
BshTI ACCGGT 1 cut(s) 414
BsiSI CCGG 2 cut(s) 21, 415
BslFI GGGAC 1 cut(s) 687
BslI CCNNNNNNNGG 2 cut(s) 435, 643
BsmAI GTCTC 3 cut(s) 529, 557, 601
BsmBI CGTCTC 1 cut(s) 529
BsmFI GGGAC 1 cut(s) 687
BsnI GGCC 2 cut(s) 173, 668
Bsp143I GATC 5 cut(s) 15, 47, 58, 150, 738
BspACI CCGC 6 cut(s) 5, 24, 30, 262, 669, 671
BspANI GGCC 2 cut(s) 173, 668
BspCNI CTCAG 2 cut(s) 203, 611
BspFNI CGCG 1 cut(s) 671
BspLI GGNNCC 2 cut(s) 49, 419
BspPI GGATC 4 cut(s) 42, 53, 55, 158
BspT107I GGYRCC 1 cut(s) 417
BsrBI CCGCTC 2 cut(s) 30, 262
BsrFI RCCGGY 2 cut(s) 20, 414
BssAI RCCGGY 2 cut(s) 20, 414
BssECI CCNNGG 2 cut(s) 76, 669
BssMI GATC 5 cut(s) 15, 47, 58, 150, 738
BssSI CACGAG 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 76
Bst2BI CACGAG 1 cut(s) 792
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 1 cut(s) 381
Bst6I CTCTTC 1 cut(s) 727
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 4 cut(s) 177, 190, 598, 829
BstDSI CCRYGG 1 cut(s) 669
BstENI CCTNNNNNAGG 1 cut(s) 433
BstF5I GGATG 3 cut(s) 231, 254, 652
BstFNI CGCG 1 cut(s) 671
BstHHI GCGC 1 cut(s) 560
BstKTI GATC 5 cut(s) 18, 50, 61, 153, 741
BstMAI GTCTC 3 cut(s) 529, 557, 601
BstMBI GATC 5 cut(s) 15, 47, 58, 150, 738
BstMWI GCNNNNNNNGC 1 cut(s) 695
BstNI CCWGG 1 cut(s) 430
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 428
BstUI CGCG 1 cut(s) 671
BstV1I GCAGC 3 cut(s) 378, 617, 758
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 47
BstYI RGATCY 1 cut(s) 47
BsuRI GGCC 2 cut(s) 173, 668
BtgI CCRYGG 1 cut(s) 669
BtrI CACGTC 1 cut(s) 611
BtsCI GGATG 3 cut(s) 231, 254, 652
BtsI GCAGTG 1 cut(s) 335
BtsIMutI CAGTG 2 cut(s) 198, 335
Cac8I GCNNGC 1 cut(s) 22
CfoI GCGC 1 cut(s) 560
Cfr10I RCCGGY 2 cut(s) 20, 414
Cfr42I CCGCGG 1 cut(s) 672
CseI GACGC 1 cut(s) 546
CspAI ACCGGT 1 cut(s) 414
CspCI CAANNNNNGTGG 2 cut(s) 107, 142
CviAII CATG 2 cut(s) 63, 198
CviJI RGCY 9 cut(s) 173, 391, 409, 428, 445, 452, 668, 689, 749
CviKI_1 RGCY 9 cut(s) 173, 391, 409, 428, 445, 452, 668, 689, 749
DdeI CTNAG 4 cut(s) 177, 190, 598, 829
DpnI GATC 5 cut(s) 17, 49, 60, 152, 740
DpnII GATC 5 cut(s) 15, 47, 58, 150, 738
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 727
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 173
EcoNI CCTNNNNNAGG 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 529
FaeI CATG 2 cut(s) 66, 201
FaiI YATR 7 cut(s) 64, 140, 199, 270, 693, 705, 780
FaqI GGGAC 1 cut(s) 687
FatI CATG 2 cut(s) 62, 197
FauI CCCGC 2 cut(s) 17, 664
Fnu4HI GCNGC 6 cut(s) 6, 263, 392, 606, 669, 747
FokI GGATG 3 cut(s) 218, 261, 659
Fsp4HI GCNGC 6 cut(s) 6, 263, 392, 606, 669, 747
FspBI CTAG 1 cut(s) 750
GlaI GCGC 1 cut(s) 559
GluI GCNGC 6 cut(s) 6, 263, 392, 606, 669, 747
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 2 cut(s) 173, 668
HapII CCGG 2 cut(s) 21, 415
HgaI GACGC 1 cut(s) 546
HhaI GCGC 1 cut(s) 560
Hin1II CATG 2 cut(s) 66, 201
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HinfI GANTC 6 cut(s) 95, 344, 509, 525, 799, 827
HpaII CCGG 2 cut(s) 21, 415
Hpy188I TCNGA 4 cut(s) 52, 456, 601, 832
Hpy188III TCNNGA 2 cut(s) 287, 575
HpyAV CCTTC 2 cut(s) 4, 523
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4IV ACGT 2 cut(s) 184, 610
HpyCH4V TGCA 8 cut(s) 328, 377, 394, 460, 487, 519, 698, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 695
HpyF3I CTNAG 4 cut(s) 177, 190, 598, 829
HpySE526I ACGT 2 cut(s) 184, 610
Hsp92II CATG 2 cut(s) 66, 201
HspAI GCGC 1 cut(s) 558
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
KspI CCGCGG 1 cut(s) 672
Kzo9I GATC 5 cut(s) 15, 47, 58, 150, 738
LmnI GCTCC 2 cut(s) 27, 442
Lsp1109I GCAGC 3 cut(s) 378, 617, 758
LweI GCATC 3 cut(s) 447, 496, 685
MaeI CTAG 1 cut(s) 750
MaeII ACGT 2 cut(s) 184, 610
MaeIII GTNAC 3 cut(s) 193, 332, 796
MalI GATC 5 cut(s) 17, 49, 60, 152, 740
MbiI CCGCTC 2 cut(s) 30, 262
MboI GATC 5 cut(s) 15, 47, 58, 150, 738
MboII GAAGA 7 cut(s) 479, 744, 767, 776, 785, 808, 836
MfeI CAATTG 1 cut(s) 133
MflI RGATCY 1 cut(s) 47
MluCI AATT 4 cut(s) 83, 133, 220, 581
MlyI GAGTC 2 cut(s) 793, 821
MreI CGCCGGCG 1 cut(s) 20
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 2 cut(s) 21, 415
MspR9I CCNGG 1 cut(s) 430
MunI CAATTG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 430
MvnI CGCG 1 cut(s) 671
MwoI GCNNNNNNNGC 1 cut(s) 695
NaeI GCCGGC 1 cut(s) 22
NdeII GATC 5 cut(s) 15, 47, 58, 150, 738
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 66, 201
NlaIV GGNNCC 2 cut(s) 49, 419
NmuCI GTSAC 2 cut(s) 193, 796
NspI RCATGY 1 cut(s) 201
PceI AGGCCT 1 cut(s) 173
PciI ACATGT 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 4 cut(s) 95, 344, 509, 525
PinAI ACCGGT 1 cut(s) 414
PkrI GCNGC 6 cut(s) 7, 264, 393, 607, 670, 748
PleI GAGTC 2 cut(s) 793, 821
PpsI GAGTC 2 cut(s) 793, 821
PscI ACATGT 1 cut(s) 197
Psp1406I AACGTT 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 428
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 2 cut(s) 49, 419
PsuI RGATCY 1 cut(s) 47
SacII CCGCGG 1 cut(s) 672
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 6 cut(s) 6, 263, 392, 606, 669, 747
Sau3AI GATC 5 cut(s) 15, 47, 58, 150, 738
SchI GAGTC 2 cut(s) 793, 821
ScrFI CCNGG 1 cut(s) 430
SfaNI GCATC 3 cut(s) 447, 496, 685
Sfr303I CCGCGG 1 cut(s) 672
SgrAI CRCCGGYG 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 672
Sse9I AATT 4 cut(s) 83, 133, 220, 581
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 6 cut(s) 5, 24, 30, 262, 669, 671
SspI AATATT 1 cut(s) 283
SspMI CTAG 1 cut(s) 750
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 428
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 1 cut(s) 381
TaiI ACGT 2 cut(s) 187, 613
TaqI TCGA 3 cut(s) 273, 369, 819
TaqII GACCGA 1 cut(s) 651
TasI AATT 4 cut(s) 83, 133, 220, 581
TauI GCSGC 3 cut(s) 8, 265, 671
TfiI GAWTC 4 cut(s) 95, 344, 509, 525
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 2 cut(s) 198, 335
TseFI GTSAC 2 cut(s) 193, 796
TseI GCWGC 3 cut(s) 391, 605, 746
Tsp45I GTSAC 2 cut(s) 193, 796
TspDTI ATGAA 1 cut(s) 663
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 2 cut(s) 198, 335
XagI CCTNNNNNAGG 1 cut(s) 433
XapI RAATTY 1 cut(s) 220
XceI RCATGY 1 cut(s) 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.