MD12G1221200.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008784
Physical Location & Seq
Reverse (-)
29759777 .. 29760342
566 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1221200.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGTATCTGCAGCTTTTTCTTATCAAAGGAGCACAAGCATAGATTGCAGTGTTATTACACTGAACCAGGTTTTGTTCTTCTTGTCATTTTATTGGTTGTGTTGTTTCTCATTTTATATCCCTGAGAAACGCCGTCACTGTTTACATGGTTTCTTAAGTGACATATTTGATGTACCACGGTATCAGAAGTCCCGCAAGTATCTTCAGCCAAACCGCTGGCTCCAAAGTTGGTGGAGAAGGGAAATCCAAGCTCTGGTGCAGGAGGAAGATGTTGACATCGTCATGCACCACATACATGGCGTGATTAATTCATCCCTGACGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4

Protein Analysis

109

Amino Acids

13.03

Weight (kDa)

8.8

Isoelectric Point (pI)

75.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 253
AciI CCGC 2 cut(s) 193, 214
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 2 cut(s) 253, 321
AflII CTTAAG 1 cut(s) 154
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 2 cut(s) 14, 251
AluI AGCT 2 cut(s) 14, 251
Alw21I GWGCWC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 11
AseI ATTAAT 1 cut(s) 306
BaeI ACNNNNGTAYC 2 cut(s) 164, 197
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 9
BfrI CTTAAG 1 cut(s) 154
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 176
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 321
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 253, 321
BseMII CTCAG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 23
BsgI GTGCAG 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 35
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 253, 321
BsmFI GGGAC 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 35
BspACI CCGC 2 cut(s) 193, 214
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 1 cut(s) 221
BspMAI CTGCAG 1 cut(s) 13
BspTI CTTAAG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 2 cut(s) 140, 180
BstAFI CTTAAG 1 cut(s) 154
BstAPI GCANNNNNTGC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 1 cut(s) 123
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 311
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 23
BstXI CCANNNNNNTGG 2 cut(s) 216, 296
BtgI CCRYGG 1 cut(s) 176
BtsCI GGATG 1 cut(s) 311
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 3 cut(s) 55, 59, 136
Cac8I GCNNGC 1 cut(s) 218
CsiI ACCWGGT 1 cut(s) 66
Csp6I GTAC 1 cut(s) 173
CspCI CAANNNNNGTGG 2 cut(s) 212, 247
CviAII CATG 3 cut(s) 146, 283, 296
CviJI RGCY 4 cut(s) 14, 208, 220, 251
CviKI_1 RGCY 4 cut(s) 14, 208, 220, 251
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 66
FaeI CATG 3 cut(s) 149, 286, 299
FaiI YATR 7 cut(s) 41, 117, 147, 164, 284, 293, 297
FaqI GGGAC 1 cut(s) 175
FatI CATG 3 cut(s) 145, 282, 295
FauI CCCGC 1 cut(s) 200
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 298
Fsp4HI GCNGC 1 cut(s) 12
GluI GCNGC 1 cut(s) 12
Hin1II CATG 3 cut(s) 149, 286, 299
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 143, 274
Hpy188I TCNGA 1 cut(s) 186
Hpy8I GTNNAC 2 cut(s) 143, 274
HpyAV CCTTC 1 cut(s) 231
HpyCH4III ACNGT 2 cut(s) 140, 180
HpyCH4V TGCA 4 cut(s) 11, 48, 259, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 123
Hsp92II CATG 3 cut(s) 149, 286, 299
LmnI GCTCC 2 cut(s) 30, 225
LpnPI CCDG 6 cut(s) 53, 80, 135, 202, 239, 245
Lsp1109I GCAGC 1 cut(s) 23
MabI ACCWGGT 1 cut(s) 66
MaeIII GTNAC 2 cut(s) 134, 158
MboII GAAGA 3 cut(s) 70, 194, 278
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 1 cut(s) 307
MnlI CCTC 2 cut(s) 256, 315
MseI TTAA 2 cut(s) 155, 306
MslI CAYNNNNRTG 2 cut(s) 281, 294
MspA1I CMGCKG 1 cut(s) 216
MspCI CTTAAG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 68
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 45
NlaIII CATG 3 cut(s) 149, 286, 299
NlaIV GGNNCC 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 134, 158
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 253
PkrI GCNGC 1 cut(s) 13
PshBI ATTAAT 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 221
PstI CTGCAG 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 2 cut(s) 281, 294
SaqAI TTAA 2 cut(s) 155, 306
SatI GCNGC 1 cut(s) 12
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 1 cut(s) 35
SetI ASST 4 cut(s) 16, 72, 253, 326
SexAI ACCWGGT 1 cut(s) 66
SfcI CTRYAG 1 cut(s) 9
SmiMI CAYNNNNRTG 2 cut(s) 281, 294
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 1 cut(s) 307
SsiI CCGC 2 cut(s) 193, 214
StyD4I CCNGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 140, 180
TasI AATT 1 cut(s) 307
Tru1I TTAA 2 cut(s) 155, 306
Tru9I TTAA 2 cut(s) 155, 306
TscAI CASTG 3 cut(s) 55, 66, 143
TseFI GTSAC 2 cut(s) 134, 158
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 2 cut(s) 134, 158
TspDTI ATGAA 1 cut(s) 300
TspRI CASTG 3 cut(s) 55, 66, 143
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 253
Vha464I CTTAAG 1 cut(s) 154
VspI ATTAAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.