RLG00000025446

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008784
Physical Location & Seq
Reverse (-)
45804840 .. 45806967
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025446

Sequence Viewer

Length: 825 bp
ATGGCGCAGGAGCAAGAGTTGAATGTGTGCAGCTCTTCTGCTTCTCCATCATCAGAGGATTTGAATCCTTGTCCCATCTGCCTCGCACCCATCTTTCGACTTTCTTATCTTGACAAATGTTTCCATAAGTTCTGTTACAAATGCATATTACAATGGACCAAAGTTGTTGCTAGCAAGCAGTCTGACCCGTCTTCCTCTGTAAAGTGTCCTTTGTGCAAGATGGATAATTTTTCGATTCTACATGGATATGATGGATGTTCGTTTCAAAAGCATTACATTAACCAGGATTCAGGGGATAGCTTTGTCTTATCAAGTGCTCACAAGTATAGGTTACAGTGCTATTATACTGAACCAGGTTCCTTAAATGACATCTTTGATGTATCACGGTATTGGAAATCCTTTAAGTATCTTCAGCCAAACTGTTGGCTTCAAAGTTGGCTGAGAAGGGAAATCCAAGCTCTTATGCAGGAAGAAGATGTTGACATCATCATGCACCACATACTTGGCGTGATTAAATCCCTAATGAGTCATAAGCAAAAAGGTCAGACGAAATCATCTGAAGCAAAACAGAGAGAATTTCAAGAGACAGTCTCCGATGCAGCAAGGCCTTTCTTAGCCACAAGAACTGACCGATTTGTAAATGAGGTGGAACTATTTCTTGCTTCAGGTTTAAACATTGAGGCATACGATGCTGTGTGCATGCAGCGCTTAGGTTGGAGTGCTCACGGAGTAACTACAGAAGCTGCTGCAGAAGGAGAACTTGCCGAACATAGACCTGTTATCCCTTACTTGCATATTTTTGATGACTTTGACTGCAGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4

Protein Analysis

275

Amino Acids

31.33

Weight (kDa)

5.68

Isoelectric Point (pI)

65.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4 PF00097 24 - 72 1.8e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 3 cut(s) 395, 579, 648
AfeI AGCGCT 1 cut(s) 707
AgsI TTSAA 5 cut(s) 22, 64, 266, 431, 581
AjnI CCWGG 2 cut(s) 282, 352
AluBI AGCT 4 cut(s) 33, 300, 458, 743
AluI AGCT 4 cut(s) 33, 300, 458, 743
Alw21I GWGCWC 2 cut(s) 319, 724
Alw26I GTCTC 2 cut(s) 578, 595
AlwNI CAGNNNCTG 1 cut(s) 743
Aor51HI AGCGCT 1 cut(s) 707
AoxI GGCC 1 cut(s) 605
ApeKI GCWGC 5 cut(s) 30, 599, 703, 743, 746
ApoI RAATTY 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 654
AspLEI GCGC 2 cut(s) 7, 708
AspS9I GGNCC 1 cut(s) 156
AsuNHI GCTAGC 1 cut(s) 170
AvaII GGWCC 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 319, 724
BbvI GCAGC 5 cut(s) 42, 611, 715, 730, 733
BccI CCATC 5 cut(s) 55, 83, 98, 214, 245
BciT130I CCWGG 2 cut(s) 284, 354
BcoDI GTCTC 2 cut(s) 578, 595
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 3 cut(s) 735, 747, 814
BfoI RGCGCY 1 cut(s) 709
BisI GCNGC 5 cut(s) 31, 600, 704, 744, 747
BlsI GCNGC 5 cut(s) 32, 601, 705, 745, 748
Bme1390I CCNGG 2 cut(s) 284, 354
Bme18I GGWCC 1 cut(s) 156
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 2 cut(s) 284, 354
BmsI GCATC 2 cut(s) 586, 679
BmtI GCTAGC 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 575, 607
Bpu10I CCTNAGC 1 cut(s) 709
BsaBI GATNNNNATC 1 cut(s) 63
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 2 cut(s) 284, 354
BseGI GGATG 1 cut(s) 260
BseJI GATNNNNATC 1 cut(s) 63
BseMII CTCAG 1 cut(s) 431
BseXI GCAGC 5 cut(s) 42, 611, 715, 730, 733
BsgI GTGCAG 1 cut(s) 49
BshFI GGCC 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 319, 724
BslFI GGGAC 1 cut(s) 57
BsmAI GTCTC 2 cut(s) 578, 595
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 1 cut(s) 607
Bsp1286I GDGCHC 2 cut(s) 319, 724
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 358
BspMAI CTGCAG 2 cut(s) 751, 818
BspOI GCTAGC 1 cut(s) 174
BspQI GCTCTTC 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 284, 354
Bst4CI ACNGT 4 cut(s) 336, 387, 422, 589
Bst6I CTCTTC 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 689
BstC8I GCNNGC 3 cut(s) 172, 176, 701
BstDEI CTNAG 3 cut(s) 440, 613, 709
BstF5I GGATG 1 cut(s) 260
BstH2I RGCGCY 1 cut(s) 709
BstHHI GCGC 2 cut(s) 7, 708
BstMAI GTCTC 2 cut(s) 578, 595
BstMWI GCNNNNNNNGC 2 cut(s) 689, 705
BstNI CCWGG 2 cut(s) 284, 354
BstNSI RCATGY 1 cut(s) 703
BstSCI CCNGG 2 cut(s) 282, 352
BstSFI CTRYAG 3 cut(s) 735, 747, 814
BstV1I GCAGC 5 cut(s) 42, 611, 715, 730, 733
BstV2I GAAGAC 1 cut(s) 183
BstXI CCANNNNNNTGG 2 cut(s) 423, 503
BsuRI GGCC 1 cut(s) 607
BtsCI GGATG 1 cut(s) 260
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 2 cut(s) 341, 823
Cac8I GCNNGC 3 cut(s) 172, 176, 701
CaiI CAGNNNCTG 1 cut(s) 743
CfoI GCGC 2 cut(s) 7, 708
Cfr13I GGNCC 1 cut(s) 156
CsiI ACCWGGT 1 cut(s) 352
CviAII CATG 3 cut(s) 242, 490, 700
CviJI RGCY 9 cut(s) 33, 300, 415, 427, 439, 458, 607, 617, 743
CviKI_1 RGCY 9 cut(s) 33, 300, 415, 427, 439, 458, 607, 617, 743
DdeI CTNAG 3 cut(s) 440, 613, 709
DraI TTTAAA 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco147I AGGCCT 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 156
Eco47III AGCGCT 1 cut(s) 707
Eco57I CTGAAG 3 cut(s) 395, 579, 648
EcoRII CCWGG 2 cut(s) 282, 352
EcoT22I ATGCAT 1 cut(s) 146
FaeI CATG 3 cut(s) 245, 493, 703
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 1 cut(s) 57
FatI CATG 3 cut(s) 241, 489, 699
Fnu4HI GCNGC 5 cut(s) 31, 600, 704, 744, 747
FokI GGATG 1 cut(s) 267
Fsp4HI GCNGC 5 cut(s) 31, 600, 704, 744, 747
FspBI CTAG 1 cut(s) 171
GlaI GCGC 2 cut(s) 6, 707
GluI GCNGC 5 cut(s) 31, 600, 704, 744, 747
HaeII RGCGCY 1 cut(s) 709
HaeIII GGCC 1 cut(s) 607
HhaI GCGC 2 cut(s) 7, 708
Hin1II CATG 3 cut(s) 245, 493, 703
Hin6I GCGC 2 cut(s) 5, 706
HinP1I GCGC 2 cut(s) 5, 706
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HinfI GANTC 4 cut(s) 64, 235, 287, 526
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 5 cut(s) 55, 184, 546, 559, 595
Hpy188III TCNNGA 2 cut(s) 110, 581
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 2 cut(s) 438, 746
HpyCH4III ACNGT 4 cut(s) 336, 387, 422, 589
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 705
HpyF3I CTNAG 3 cut(s) 440, 613, 709
Hsp92II CATG 3 cut(s) 245, 493, 703
HspAI GCGC 2 cut(s) 5, 706
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 8 cut(s) 269, 276, 296, 339, 366, 452, 651, 789
Lsp1109I GCAGC 5 cut(s) 42, 611, 715, 730, 733
LweI GCATC 2 cut(s) 586, 679
MabI ACCWGGT 1 cut(s) 352
MaeI CTAG 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 134, 330, 730
MboII GAAGA 5 cut(s) 27, 183, 401, 482, 485
MhlI GDGCHC 2 cut(s) 319, 724
MluCI AATT 2 cut(s) 226, 575
MlyI GAGTC 1 cut(s) 535
MmeI TCCRAC 1 cut(s) 695
MnlI CCTC 5 cut(s) 49, 92, 205, 637, 673
Mph1103I ATGCAT 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 5 cut(s) 279, 362, 402, 513, 671
MslI CAYNNNNRTG 2 cut(s) 246, 488
MspR9I CCNGG 2 cut(s) 284, 354
MssI GTTTAAAC 1 cut(s) 672
MvaI CCWGG 2 cut(s) 284, 354
MwoI GCNNNNNNNGC 2 cut(s) 689, 705
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 3 cut(s) 245, 493, 703
NlaIV GGNNCC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 146
NspI RCATGY 1 cut(s) 703
PaeI GCATGC 1 cut(s) 703
PceI AGGCCT 1 cut(s) 607
PciSI GCTCTTC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 3 cut(s) 64, 235, 287
PkrI GCNGC 5 cut(s) 32, 601, 705, 745, 748
PleI GAGTC 1 cut(s) 534
PmeI GTTTAAAC 1 cut(s) 672
PpsI GAGTC 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 282, 352
PspGI CCWGG 2 cut(s) 282, 352
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 1 cut(s) 156
PstI CTGCAG 2 cut(s) 751, 818
PstNI CAGNNNCTG 1 cut(s) 743
RseI CAYNNNNRTG 2 cut(s) 246, 488
SapI GCTCTTC 1 cut(s) 40
SaqAI TTAA 5 cut(s) 279, 362, 402, 513, 671
SatI GCNGC 5 cut(s) 31, 600, 704, 744, 747
Sau96I GGNCC 1 cut(s) 156
SchI GAGTC 1 cut(s) 535
ScrFI CCNGG 2 cut(s) 284, 354
SduI GDGCHC 2 cut(s) 319, 724
SexAI ACCWGGT 1 cut(s) 352
SfaNI GCATC 2 cut(s) 586, 679
SfcI CTRYAG 3 cut(s) 735, 747, 814
SinI GGWCC 1 cut(s) 156
SmiMI CAYNNNNRTG 2 cut(s) 246, 488
SphI GCATGC 1 cut(s) 703
Sse9I AATT 2 cut(s) 226, 575
SseBI AGGCCT 1 cut(s) 607
SspMI CTAG 1 cut(s) 171
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 2 cut(s) 282, 352
TaaI ACNGT 4 cut(s) 336, 387, 422, 589
TaqI TCGA 2 cut(s) 97, 233
TaqII GACCGA 1 cut(s) 645
TasI AATT 2 cut(s) 226, 575
TfiI GAWTC 3 cut(s) 64, 235, 287
Tru1I TTAA 5 cut(s) 279, 362, 402, 513, 671
Tru9I TTAA 5 cut(s) 279, 362, 402, 513, 671
TscAI CASTG 2 cut(s) 341, 823
TseI GCWGC 5 cut(s) 30, 599, 703, 743, 746
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 2 cut(s) 341, 823
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 1 cut(s) 575
XceI RCATGY 1 cut(s) 703
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 1 cut(s) 171
Zsp2I ATGCAT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.