AT3G10690

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010284
Physical Location & Seq
Reverse (-)
3339392 .. 3346360
6969 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10690.1

Sequence Viewer

Length: 2853 bp
ATGACTCCAGTATTATGCCATTCCACTGCTTCAATCCCTAACCCTAACTCTCTCATGTCTCTCTCCTCCACTCTCCGTCTCTCTTCCTCCCTTCTTCGCCGCTCCTTCTTCCGATTCCCCCTCACCGACCCATTATGCCGTCTCCGCCGCACTGAACCCTCCGCCACGCGTTTCTTCTCCTCCCGTACTCCTCGGTCCGGCAAATTCGTTGTCGGTGCTGGTAAGCGCGGAGATGAGCAGGTCAAGGAAGAGTCCGGAGCTAACAATGGCGGTTTGGTTGTTTCTGGGGACGAATCTCGTATTGTGCCTTTTGAGCTACATAAGGAAGCTACTGAGTCGTATATGTCGTATGCGCTCTCTGTTTTGCTTGGACGTGCTTTGCCTGATGTTAGAGACGGACTAAAACCAGTACATCGGAGAATTCTCTTTGCAATGCATGAGTTGGGGATGTCATCTAAAAAACCATACAAGAAATGTGCAAGAGTTGTTGGAGAGGTTCTTGGTAAATTCCATCCGCATGGAGATACTGCTGTTTATGATTCTCTTGTTAGGATGGCTCAGAGTTTCTCCTTGAGATGTCCACTTATCCAAGGCCATGGAAATTTTGGCTCAATAGATGCAGATCCTCCTGCTGCTATGCGTTACACTGAATGCAGACTCGATCCACTGGCAGAGGCAGTACTATTGTCTGATCTGGATCAAGATACGGTGGATTTTGTGGCCAATTTTGATAACTCACAAAAAGAACCAGCAGTGTTGCCTGCTCGTCTTCCTGCTTTGTTGTTGAATGGAGCTTCTGGGATTGCGGTTGGCATGGCAACAAATATTCCCCCTCATAATCTTGGGGAGTTGGTAGACGTGCTTTGCGCTTTAATCCATAATCCAGAAGCAACGCTGCAAGAACTGCTGGAATACATGCCTGCACCGGATTTTCCAACTGGAGGAATAATAATGGGAAACCTTGGGGTTTTGGATGCTTACCGAACTGGCCGAGGGCGAGTTGTAGTTAGAGGAAAAGCTGAAGTTGAATTGTTGGATCCAAAGACAAAGCGGAATGCAGTTATTATCACAGAGATTCCTTACCAGACAAACAAAGCTACACTTGTCCAGAAGATTGCCGAACTTGTTGAAAATAAGACGCTAGAGGGCATAAGTGACATACGTGACGAGAGTGATCGTAATGGGATGCGTGTGGTTATTGAGCTCAAAAGAGGAGGGGATCCTGCACTTGTGCTTAATAATCTTTACCGGCATACTGCCCTTCAATCAAGCTTCAGCTGCAACATGGTGGGTATCTGCGATGGAGAACCCAAGCTAATGGGATTAAAAGAACTACTTCAGGCATTTATAGATTTCAGGTGTTCTGTCGTCGAAAGGCGTGCAAGATTCAAGCTTTCACATGCACAACAACGAAAGCATATTATTGAGGGTATTGTGGTTGGGCTTGACAATGTGGACGAAGTAATCGAACTCATCACGAAGGCCTCAAGTCATTCCTCTGCTACTGCTGCTTTACAAAGTGAATATGGTCTTTCTGAGAAACAAGCTGAGGCTATACTGGAGATAACTCTTCGAAGGCTAACTGCTCTTGAGCGGAAAAAGTTTACTGATGAAAGTAGTTCACTAACAGAGCAAATTACAAAGTTGGAGCAGCTATTATCAACCCGAACAAACATCCTAAAGTTAATCGAACAAGAAGCAATAGAGCTGAAGGACAGATTCTCAAGTCCCAGGCGTTCAATGTTGGAGGATTCTGACAGTGGTGATCTAGAAGATATAGATGTTATTCCAAATGAAGAGATGCTGATGGCAGTCAGCGAAAAGGGCTATGTGAAGAGAATGAAGGCCGATACATTCAATCTTCAGCATCGTGGAACAATTGGCAAATCTGTTGGGAAACTAAGAGTTGATGATGCAATGTCTGACTTTCTTGTTTGTCATGCACATGACCATGTCCTCTTTTTCAGTGACCGGGGTATAGTTTACTCAACCCGTGCATATAAAATTCCAGAATGCTCTCGCAATGCAGCTGGTACACCTTTGGTTCAGATATTATCCATGTCGGAAGGTGAAAGAGTAACCTCCATTGTTCCTGTTAGCGAGTTTGCTGAAGATCGCTACCTCCTGATGCTTACAGTGAATGGCTGCATCAAGAAAGTATCTTTGAAATTATTTTCAGGAATACGCTCAACTGGAATCATAGCAATTCAGTTGAATTCTGGTGATGAACTGAAATGGGTTCGTTGTTGCTCAAGCGACGATCTTGTAGCCATGGCATCCCAAAATGGAATGGTCGCCTTGAGTACATGTGATGGTGTTCGCACACTGAGCAGAAATACAAAGGGAGTGACTGCCATGAGACTCAAGAATGAGGATAAGATAGCGAGCATGGACATCATACCTGCATCTCTGCGGAAAGATATGGAAGAGAAGTCGGAAGATGCTTCACTTGTGAAACAATCCACGGGTCCATGGCTATTATTCGTGTGTGAGAATGGCTATGGAAAGCGTGTTCCTCTGAGTAGCTTCCGGCGTTCGCGTCTGAACAGAGTTGGTTTATCTGGATACAAGTTTGCTGAGGATGATCGTTTGGCTGCTGTTTTCGTTGTTGGTTACTCCTTGGCTGAGGATGGGGAGAGTGATGAGCAAGTAGTTCTGGTGAGCCAAAGCGGAACTGTGAACCGGATCAAAGTCAGGGATATCTCAATCCAATCTCGCAGAGCACGGGGAGTGATTCTTATGCGGCTAGATCATGCCGGCAAAATCCAATCTGCATCTCTGATCTCTGCAGCAGATGAAGAAGAAACAGAAGGAACTCTAAGTAATGAAGCCGTAGAAGCCGTTAGCCTGTAA

Protein Analysis

950

Amino Acids

104.54

Weight (kDa)

6.47

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 125 - 566 2.3e-160 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 600 - 646 1.8e-09 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 650 - 700 7.1e-10 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 707 - 751 1.2e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 754 - 800 1.3e-09 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 825 - 870 2.9e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 883 - 928 2.1e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 229, 2410
AccBSI CCGCTC 2 cut(s) 102, 1594
AccI GTMKAC 1 cut(s) 855
AccII CGCG 3 cut(s) 169, 228, 2539
AccIII TCCGGA 1 cut(s) 254
AclWI GGATC 8 cut(s) 617, 656, 705, 1031, 1044, 1214, 1227, 2693
AcoI YGGCCR 2 cut(s) 720, 988
AcsI RAATTY 6 cut(s) 203, 420, 506, 601, 2004, 2215
AcuI CTGAAG 6 cut(s) 1041, 1258, 1322, 1730, 1847, 2130
AfaI GTAC 5 cut(s) 187, 411, 681, 2035, 2305
AfiI CCNNNNNNNGG 2 cut(s) 197, 941
AflIII ACRYGT 2 cut(s) 167, 2306
AjiI CACGTC 2 cut(s) 374, 859
AjnI CCWGG 1 cut(s) 1730
AloI GAACNNNNNNTCC 2 cut(s) 2496, 2528
Alw21I GWGCWC 2 cut(s) 1206, 2725
Alw26I GTCTC 5 cut(s) 63, 83, 146, 387, 2353
AlwI GGATC 8 cut(s) 617, 656, 705, 1031, 1044, 1214, 1227, 2693
Aor13HI TCCGGA 1 cut(s) 254
AoxI GGCC 5 cut(s) 592, 720, 988, 1482, 1845
ApeKI GCWGC 9 cut(s) 632, 895, 1278, 1508, 1651, 2027, 2145, 2594, 2789
ApoI RAATTY 6 cut(s) 203, 420, 506, 601, 2004, 2215
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
Asp700I GAANNNNTTC 1 cut(s) 1335
AspLEI GCGC 3 cut(s) 228, 355, 869
AspS9I GGNCC 2 cut(s) 195, 2468
AsuC2I CCSGG 1 cut(s) 1973
AsuHPI GGTGA 5 cut(s) 115, 1775, 2081, 2234, 2671
AsuII TTCGAA 1 cut(s) 1573
AvaII GGWCC 2 cut(s) 195, 2468
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BalI TGGCCA 1 cut(s) 722
BamHI GGATCC 2 cut(s) 1036, 1219
BanII GRGCYC 1 cut(s) 1206
BbsI GAAGAC 1 cut(s) 761
Bbv12I GWGCWC 2 cut(s) 1206, 2725
BbvCI CCTCAGC 3 cut(s) 1548, 2577, 2625
BbvI GCAGC 9 cut(s) 619, 882, 1265, 1495, 1663, 2039, 2132, 2581, 2801
BccI CCATC 6 cut(s) 519, 547, 1295, 1801, 2306, 2624
BceAI ACGGC 3 cut(s) 123, 2816, 2825
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BciT130I CCWGG 1 cut(s) 1732
BciVI GTATCC 1 cut(s) 2558
BcnI CCSGG 1 cut(s) 1973
BcoDI GTCTC 5 cut(s) 63, 83, 146, 387, 2353
BfaI CTAG 3 cut(s) 1142, 1769, 2747
BfmI CTRYAG 1 cut(s) 2787
BfuAI ACCTGC 2 cut(s) 229, 2410
BfuI GTATCC 1 cut(s) 2558
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 2 cut(s) 1732, 1973
Bme18I GGWCC 2 cut(s) 195, 2468
BmgBI CACGTC 2 cut(s) 374, 859
BmgT120I GGNCC 2 cut(s) 195, 2468
BmiI GGNNCC 3 cut(s) 1038, 1221, 2469
BmrFI CCNGG 2 cut(s) 1732, 1973
BpiI GAAGAC 1 cut(s) 761
BpmI CTGGAG 2 cut(s) 960, 1580
Bpu10I CCTNAGC 3 cut(s) 1548, 2577, 2625
Bpu14I TTCGAA 1 cut(s) 1573
BpuEI CTTGAG 7 cut(s) 592, 1471, 1610, 1708, 2236, 2320, 2348
BpuMI CCSGG 1 cut(s) 1973
BsaAI YACGTR 1 cut(s) 1163
BsaBI GATNNNNATC 2 cut(s) 621, 696
BsaWI WCCGGW 3 cut(s) 254, 925, 2682
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 941
Bse118I RCCGGY 2 cut(s) 1248, 2756
Bse1I ACTGG 7 cut(s) 8, 407, 672, 943, 991, 1563, 2197
Bse3DI GCAATG 3 cut(s) 438, 1923, 2029
Bse8I GATNNNNATC 2 cut(s) 621, 696
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 1 cut(s) 1732
BseGI GGATG 9 cut(s) 453, 511, 558, 979, 1191, 1674, 2276, 2587, 2635
BseJI GATNNNNATC 2 cut(s) 621, 696
BseLI CCNNNNNNNGG 2 cut(s) 197, 941
BseMI GCAATG 3 cut(s) 438, 1923, 2029
BseMII CTCAG 8 cut(s) 324, 572, 1527, 1539, 2318, 2510, 2568, 2616
BseNI ACTGG 7 cut(s) 8, 407, 672, 943, 991, 1563, 2197
BseRI GAGGAG 4 cut(s) 55, 169, 180, 1227
BseXI GCAGC 9 cut(s) 619, 882, 1265, 1495, 1663, 2039, 2132, 2581, 2801
BsgI GTGCAG 2 cut(s) 906, 1209
Bsh1236I CGCG 3 cut(s) 169, 228, 2539
BshFI GGCC 5 cut(s) 594, 722, 990, 1484, 1847
BsiHKAI GWGCWC 2 cut(s) 1206, 2725
BsiSI CCGG 8 cut(s) 198, 255, 926, 1249, 1972, 2530, 2683, 2757
BslFI GGGAC 2 cut(s) 302, 1713
BslI CCNNNNNNNGG 2 cut(s) 197, 941
BsmAI GTCTC 5 cut(s) 63, 83, 146, 387, 2353
BsmBI CGTCTC 3 cut(s) 83, 146, 387
BsmFI GGGAC 2 cut(s) 302, 1713
BsmI GAATGC 3 cut(s) 656, 1060, 2018
BsnI GGCC 5 cut(s) 594, 722, 990, 1484, 1847
Bsp119I TTCGAA 1 cut(s) 1573
Bsp1286I GDGCHC 2 cut(s) 1206, 2725
Bsp13I TCCGGA 1 cut(s) 254
Bsp19I CCATGG 3 cut(s) 595, 2271, 2471
BspANI GGCC 5 cut(s) 594, 722, 990, 1484, 1847
BspCNI CTCAG 8 cut(s) 325, 571, 1528, 1540, 2319, 2511, 2569, 2617
BspEI TCCGGA 1 cut(s) 254
BspFNI CGCG 3 cut(s) 169, 228, 2539
BspLI GGNNCC 3 cut(s) 1038, 1221, 2469
BspMAI CTGCAG 1 cut(s) 2791
BspMI ACCTGC 2 cut(s) 229, 2410
BspPI GGATC 8 cut(s) 617, 656, 705, 1031, 1044, 1214, 1227, 2693
BspT104I TTCGAA 1 cut(s) 1573
BsrBI CCGCTC 2 cut(s) 102, 1594
BsrDI GCAATG 3 cut(s) 438, 1923, 2029
BsrFI RCCGGY 2 cut(s) 1248, 2756
BsrI ACTGG 7 cut(s) 8, 407, 672, 943, 991, 1563, 2197
BssAI RCCGGY 2 cut(s) 1248, 2756
BssT1I CCWWGG 6 cut(s) 589, 595, 961, 2271, 2471, 2619
Bst2UI CCWGG 1 cut(s) 1732
Bst4CI ACNGT 4 cut(s) 709, 1760, 2137, 2677
Bst6I CTCTTC 6 cut(s) 88, 243, 1575, 1791, 1829, 2421
BstAPI GCANNNNNTGC 1 cut(s) 904
BstBAI YACGTR 1 cut(s) 1163
BstBI TTCGAA 1 cut(s) 1573
BstC8I GCNNGC 5 cut(s) 762, 921, 1380, 2386, 2758
BstDSI CCRYGG 4 cut(s) 595, 2271, 2463, 2471
BstF5I GGATG 9 cut(s) 453, 511, 558, 979, 1191, 1674, 2276, 2587, 2635
BstFNI CGCG 3 cut(s) 169, 228, 2539
BstHHI GCGC 3 cut(s) 228, 355, 869
BstMAI GTCTC 5 cut(s) 63, 83, 146, 387, 2353
BstMWI GCNNNNNNNGC 8 cut(s) 144, 313, 904, 1278, 1508, 1824, 2328, 2837
BstNI CCWGG 1 cut(s) 1732
BstNSI RCATGY 3 cut(s) 919, 1403, 2310
BstSCI CCNGG 2 cut(s) 1730, 1971
BstSFI CTRYAG 1 cut(s) 2787
BstUI CGCG 3 cut(s) 169, 228, 2539
BstV1I GCAGC 9 cut(s) 619, 882, 1265, 1495, 1663, 2039, 2132, 2581, 2801
BstV2I GAAGAC 1 cut(s) 761
BstX2I RGATCY 3 cut(s) 622, 1036, 1219
BstXI CCANNNNNNTGG 3 cut(s) 518, 596, 1318
BstYI RGATCY 3 cut(s) 622, 1036, 1219
BsuI GTATCC 1 cut(s) 2558
BsuRI GGCC 5 cut(s) 594, 722, 990, 1484, 1847
BtgI CCRYGG 4 cut(s) 595, 2271, 2463, 2471
BtgZI GCGATG 1 cut(s) 1314
BtrI CACGTC 2 cut(s) 374, 859
BtsCI GGATG 9 cut(s) 453, 511, 558, 979, 1191, 1674, 2276, 2587, 2635
BtsI GCAGTG 2 cut(s) 24, 759
BtsIMutI CAGTG 9 cut(s) 24, 150, 645, 665, 759, 1765, 1972, 2142, 2324
BveI ACCTGC 2 cut(s) 229, 2410
Cac8I GCNNGC 5 cut(s) 762, 921, 1380, 2386, 2758
CfoI GCGC 3 cut(s) 228, 355, 869
Cfr10I RCCGGY 2 cut(s) 1248, 2756
Cfr13I GGNCC 2 cut(s) 195, 2468
CpoI CGGWCCG 1 cut(s) 195
CseI GACGC 2 cut(s) 1147, 2528
Csp6I GTAC 5 cut(s) 186, 410, 680, 2034, 2304
CspI CGGWCCG 1 cut(s) 195
CviQI GTAC 5 cut(s) 186, 410, 680, 2034, 2304
EaeI YGGCCR 2 cut(s) 720, 988
Eam1104I CTCTTC 6 cut(s) 88, 243, 1575, 1791, 1829, 2421
EarI CTCTTC 6 cut(s) 88, 243, 1575, 1791, 1829, 2421
EciI GGCGGA 2 cut(s) 134, 151
Ecl136II GAGCTC 1 cut(s) 1204
Eco130I CCWWGG 6 cut(s) 589, 595, 961, 2271, 2471, 2619
Eco147I AGGCCT 1 cut(s) 1484
Eco24I GRGCYC 1 cut(s) 1206
Eco32I GATATC 1 cut(s) 2701
Eco47I GGWCC 2 cut(s) 195, 2468
Eco53kI GAGCTC 1 cut(s) 1204
Eco57I CTGAAG 6 cut(s) 1041, 1258, 1322, 1730, 1847, 2130
EcoICRI GAGCTC 1 cut(s) 1204
EcoRI GAATTC 2 cut(s) 420, 2215
EcoRII CCWGG 1 cut(s) 1730
EcoRV GATATC 1 cut(s) 2701
EcoT14I CCWWGG 6 cut(s) 589, 595, 961, 2271, 2471, 2619
EcoT22I ATGCAT 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 1206
ErhI CCWWGG 6 cut(s) 589, 595, 961, 2271, 2471, 2619
Esp3I CGTCTC 3 cut(s) 83, 146, 387
FalI AAGNNNNNCTT 4 cut(s) 1086, 1118, 1320, 1352
FaqI GGGAC 2 cut(s) 302, 1713
FblI GTMKAC 1 cut(s) 855
FokI GGATG 9 cut(s) 460, 498, 565, 986, 1198, 1661, 2263, 2594, 2642
FriOI GRGCYC 1 cut(s) 1206
FspBI CTAG 3 cut(s) 1142, 1769, 2747
GlaI GCGC 3 cut(s) 227, 354, 868
GsuI CTGGAG 2 cut(s) 960, 1580
HaeIII GGCC 5 cut(s) 594, 722, 990, 1484, 1847
HapII CCGG 8 cut(s) 198, 255, 926, 1249, 1972, 2530, 2683, 2757
HgaI GACGC 2 cut(s) 1147, 2528
HhaI GCGC 3 cut(s) 228, 355, 869
Hin6I GCGC 3 cut(s) 226, 353, 867
HinP1I GCGC 3 cut(s) 226, 353, 867
HindIII AAGCTT 2 cut(s) 1270, 1391
HpaII CCGG 8 cut(s) 198, 255, 926, 1249, 1972, 2530, 2683, 2757
HphI GGTGA 5 cut(s) 115, 1775, 2081, 2234, 2671
Hpy166II GTNNAC 8 cut(s) 581, 856, 1456, 1605, 1622, 1984, 2036, 2680
Hpy8I GTNNAC 8 cut(s) 581, 856, 1456, 1605, 1622, 1984, 2036, 2680
Hpy99I CGWCG 2 cut(s) 1373, 2261
HpyAV CCTTC 9 cut(s) 101, 115, 1271, 1474, 1569, 1705, 1837, 2060, 2804
HpyCH4III ACNGT 4 cut(s) 709, 1760, 2137, 2677
HpyCH4IV ACGT 3 cut(s) 373, 858, 1162
HpyF10VI GCNNNNNNNGC 8 cut(s) 144, 313, 904, 1278, 1508, 1824, 2328, 2837
HpySE526I ACGT 3 cut(s) 373, 858, 1162
HspAI GCGC 3 cut(s) 226, 353, 867
Kpn2I TCCGGA 1 cut(s) 254
KroI GCCGGC 1 cut(s) 2756
KroNI GCCGGC 1 cut(s) 2758
LmnI GCTCC 4 cut(s) 107, 257, 791, 1648
Lsp1109I GCAGC 9 cut(s) 619, 882, 1265, 1495, 1663, 2039, 2132, 2581, 2801
MaeI CTAG 3 cut(s) 1142, 1769, 2747
MaeII ACGT 3 cut(s) 373, 858, 1162
MaeIII GTNAC 7 cut(s) 641, 1154, 1163, 1967, 2077, 2347, 2612
MbiI CCGCTC 2 cut(s) 102, 1594
MfeI CAATTG 1 cut(s) 1878
MflI RGATCY 3 cut(s) 622, 1036, 1219
MhlI GDGCHC 2 cut(s) 1206, 2725
MlsI TGGCCA 1 cut(s) 722
MluI ACGCGT 1 cut(s) 167
MluNI TGGCCA 1 cut(s) 722
MlyI GAGTC 4 cut(s) 260, 344, 651, 2355
MmeI TCCRAC 7 cut(s) 469, 959, 1014, 1626, 1725, 2043, 2415
Mox20I TGGCCA 1 cut(s) 722
Mph1103I ATGCAT 1 cut(s) 438
MroI TCCGGA 1 cut(s) 254
MroNI GCCGGC 1 cut(s) 2756
MroXI GAANNNNTTC 1 cut(s) 1335
MscI TGGCCA 1 cut(s) 722
MseI TTAA 4 cut(s) 872, 1236, 1325, 1685
MslI CAYNNNNRTG 3 cut(s) 516, 1944, 1950
Msp20I TGGCCA 1 cut(s) 722
MspA1I CMGCKG 2 cut(s) 1278, 2030
MspI CCGG 8 cut(s) 198, 255, 926, 1249, 1972, 2530, 2683, 2757
MspR9I CCNGG 2 cut(s) 1732, 1973
MunI CAATTG 1 cut(s) 1878
Mva1269I GAATGC 3 cut(s) 656, 1060, 2018
MvaI CCWGG 1 cut(s) 1732
MvnI CGCG 3 cut(s) 169, 228, 2539
MwoI GCNNNNNNNGC 8 cut(s) 144, 313, 904, 1278, 1508, 1824, 2328, 2837
NaeI GCCGGC 1 cut(s) 2758
NciI CCSGG 1 cut(s) 1973
NcoI CCATGG 3 cut(s) 595, 2271, 2471
NgoMIV GCCGGC 1 cut(s) 2756
NlaIV GGNNCC 3 cut(s) 1038, 1221, 2469
NmeAIII GCCGAG 1 cut(s) 1016
NmuCI GTSAC 4 cut(s) 1154, 1163, 1967, 2347
NsiI ATGCAT 1 cut(s) 438
NspI RCATGY 3 cut(s) 919, 1403, 2310
NspV TTCGAA 1 cut(s) 1573
PceI AGGCCT 1 cut(s) 1484
PciI ACATGT 1 cut(s) 2306
PcsI WCGNNNNNNNCGW 2 cut(s) 344, 1464
PctI GAATGC 3 cut(s) 656, 1060, 2018
PdiI GCCGGC 1 cut(s) 2758
PdmI GAANNNNTTC 1 cut(s) 1335
PfeI GAWTC 9 cut(s) 114, 293, 539, 1075, 1386, 1719, 1751, 2196, 2734
PflFI GACNNNGTC 1 cut(s) 1952
PleI GAGTC 4 cut(s) 259, 343, 651, 2355
PpsI GAGTC 4 cut(s) 259, 343, 651, 2355
Ppu21I YACGTR 1 cut(s) 1163
PscI ACATGT 1 cut(s) 2306
Psp124BI GAGCTC 1 cut(s) 1206
Psp6I CCWGG 1 cut(s) 1730
PspGI CCWGG 1 cut(s) 1730
PspN4I GGNNCC 3 cut(s) 1038, 1221, 2469
PspPI GGNCC 2 cut(s) 195, 2468
PstI CTGCAG 1 cut(s) 2791
PsuI RGATCY 3 cut(s) 622, 1036, 1219
PsyI GACNNNGTC 1 cut(s) 1952
PvuII CAGCTG 2 cut(s) 1278, 2030
RsaI GTAC 5 cut(s) 187, 411, 681, 2035, 2305
RsaNI GTAC 5 cut(s) 186, 410, 680, 2034, 2304
RseI CAYNNNNRTG 3 cut(s) 516, 1944, 1950
Rsr2I CGGWCCG 1 cut(s) 195
RsrII CGGWCCG 1 cut(s) 195
SacI GAGCTC 1 cut(s) 1206
SaqAI TTAA 4 cut(s) 872, 1236, 1325, 1685
Sau96I GGNCC 2 cut(s) 195, 2468
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 4 cut(s) 260, 344, 651, 2355
ScrFI CCNGG 2 cut(s) 1732, 1973
SduI GDGCHC 2 cut(s) 1206, 2725
SfcI CTRYAG 1 cut(s) 2787
SfuI TTCGAA 1 cut(s) 1573
SinI GGWCC 2 cut(s) 195, 2468
SmiMI CAYNNNNRTG 3 cut(s) 516, 1944, 1950
SmlI CTYRAG 7 cut(s) 571, 1486, 1589, 1723, 2251, 2299, 2363
SmoI CTYRAG 7 cut(s) 571, 1486, 1589, 1723, 2251, 2299, 2363
SseBI AGGCCT 1 cut(s) 1484
SspI AATATT 1 cut(s) 826
SspMI CTAG 3 cut(s) 1142, 1769, 2747
SstI GAGCTC 1 cut(s) 1206
StuI AGGCCT 1 cut(s) 1484
StyD4I CCNGG 2 cut(s) 1730, 1971
StyI CCWWGG 6 cut(s) 589, 595, 961, 2271, 2471, 2619
TaaI ACNGT 4 cut(s) 709, 1760, 2137, 2677
TaiI ACGT 3 cut(s) 376, 861, 1165
TaqI TCGA 5 cut(s) 660, 1371, 1467, 1573, 1689
TaqII GACCGA 1 cut(s) 183
TatI WGTACW 3 cut(s) 409, 679, 2303
TauI GCSGC 3 cut(s) 102, 150, 2746
TfiI GAWTC 9 cut(s) 114, 293, 539, 1075, 1386, 1719, 1751, 2196, 2734
Tru1I TTAA 4 cut(s) 872, 1236, 1325, 1685
Tru9I TTAA 4 cut(s) 872, 1236, 1325, 1685
TscAI CASTG 9 cut(s) 31, 157, 652, 672, 759, 1765, 1972, 2142, 2331
TseFI GTSAC 4 cut(s) 1154, 1163, 1967, 2347
TseI GCWGC 9 cut(s) 632, 895, 1278, 1508, 1651, 2027, 2145, 2594, 2789
Tsp45I GTSAC 4 cut(s) 1154, 1163, 1967, 2347
TspDTI ATGAA 6 cut(s) 1626, 1809, 1856, 2241, 2811, 2841
TspGWI ACGGA 2 cut(s) 65, 411
TspRI CASTG 9 cut(s) 31, 157, 652, 672, 759, 1765, 1972, 2142, 2331
Tth111I GACNNNGTC 1 cut(s) 1952
VpaK11BI GGWCC 2 cut(s) 195, 2468
XapI RAATTY 6 cut(s) 203, 420, 506, 601, 2004, 2215
XbaI TCTAGA 1 cut(s) 1768
XceI RCATGY 3 cut(s) 919, 1403, 2310
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 855
XmnI GAANNNNTTC 1 cut(s) 1335
XspI CTAG 3 cut(s) 1142, 1769, 2747
ZrmI AGTACT 1 cut(s) 681
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.