Rw1G009370

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010284
Physical Location & Seq
Forward (+)
20598240 .. 20611944
13705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009370.1

Sequence Viewer

Length: 2700 bp
ATGAAATCCTCAGTCTCGGCCATAACCCCTACCCCAACCCCCAACATGGCCTTAGCCTCCGGTCTCCGCCTCTCTTCCTCTATCCTCCGTTACCGCCTCCCTGCACCGCTCAACTCCACCCGCTTCTGCGGCCTCCGCCACAGCCTCTCCGACCTCCGATTCCTCTCAGCTTCCTCCTCTCGTACTAGGCCCCAGCTCCGCTCCATCAAAGCGAGGCCCCGCGACGACGACGACGACCTCCTCGCAGGCGGCGAGGGGCAGAATGGGAATGGGAGCGTCCTTGTCAAGGACTCAGACGAGAGAATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCTGTACTGCTCGGCCGTGCCCTGCCGGACGTTCGCGACGGTCTTAAGCCAGTGCACCGCCGCATTCTGTATGCAATGCATGAATTGAGGCTTTCGTCCCGAAAACCATTCGTGAAATGTTTTCATCCTCATGGAGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCTTTACGATCCCCACTGATTCAAGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCGCTGACCGAAGCAATGTTGCTGTCAGATTTAGAACAGGATACGGTTGATTTTGTTCCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGCCTTCCAAATTTATTATTGAATGGTTCTTCAGGGATTGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAATTGGTGGATGTTCTCAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTGGAATACATGCCAGGACCTGATTTTCCAACTGGAGGAGTGATTATGGGCAACCTTGGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTATTGTGGTCAGAGGAAAAACTGATATAGAATCTCTTGATTCTAAAGGCAAGCGAAGTGCAATCATCATAAAGGAGGTCCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATTGCACAACTTGTAGAGGATAAGATTTTAGATGGCATTAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTGATTGAGCTCAAGCGAGGATCCGACCCATCTATTGTTCTGAATAATCTATATCGCCTCACATCTCTCCAGTCCAGCTTCAGCTGTAACATGGTTGGTATTCTCAACGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCATTCCTAGACTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATTGCTGTAGGCCTTGATAATTTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCATCTCTTCTAAGAAATGAGTTCAACCTTTCTGAGAAACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTATGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAAAAACATATATTACAGGTGGTTGGACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAGGGGTATGTTAAGCGGATGAAGCCCAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTTTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGCAGCCGCACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACATCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAATGGCTACATAAAGAAGGTGTCTTTGAGTTCTTTTTCATCAATCAGGTCGACCGGAATCATAGCAATTCAACTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTGGCAGTTATACTTGTAAGGCTAGAAGTCTGAAAGGCCCGTGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCGTCTGAACAGAGTGGGTCTGATAGGTTACAAGTTTTCTGCAGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTAGTAAGCCAAAGTGGTACTGTGAATAGAATTAAGGTGCGGGATATCTCAATACAGTCTCGCTATGCAAGGGGTGTTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAAGCTGCAATTCATGGTTGA

Protein Analysis

899

Amino Acids

99.23

Weight (kDa)

7.26

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 126 - 555 2.5e-144 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 591 - 637 2.3e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 641 - 691 3.3e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 698 - 741 3.7e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 777 - 822 8.7e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 835 - 880 2.5e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 742
Acc65I GGTACC 1 cut(s) 1847
AccB1I GGYRCC 2 cut(s) 1847, 2656
AccBSI CCGCTC 2 cut(s) 109, 201
AccI GTMKAC 2 cut(s) 1956, 2162
AccII CGCG 2 cut(s) 222, 396
AclWI GGATC 4 cut(s) 543, 590, 1182, 1195
AcoI YGGCCR 2 cut(s) 18, 373
AcsI RAATTY 5 cut(s) 574, 748, 1358, 1608, 1977
AcuI CTGAAG 6 cut(s) 753, 1231, 1312, 1528, 1771, 1921
AdeI CACNNNGTG 1 cut(s) 624
AfaI GTAC 6 cut(s) 184, 366, 1849, 1934, 2008, 2530
AfiI CCNNNNNNNGG 4 cut(s) 46, 286, 328, 914
AflII CTTAAG 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 1824
AjiI CACGTC 1 cut(s) 1352
AjnI CCWGG 2 cut(s) 892, 2191
AleI CACNNNNGTG 1 cut(s) 503
AloI GAACNNNNNNTCC 6 cut(s) 565, 597, 2352, 2384, 2493, 2525
Alw21I GWGCWC 3 cut(s) 321, 417, 1179
Alw26I GTCTC 5 cut(s) 19, 68, 489, 1342, 2574
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 4 cut(s) 543, 590, 1182, 1195
AlwNI CAGNNNCTG 2 cut(s) 899, 2003
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 9 cut(s) 602, 605, 1070, 1309, 1989, 2000, 2220, 2450, 2684
ApoI RAATTY 5 cut(s) 574, 748, 1358, 1608, 1977
AseI ATTAAT 1 cut(s) 2636
Asp718I GGTACC 1 cut(s) 1847
AspLEI GCGC 1 cut(s) 640
AspS9I GGNCC 9 cut(s) 189, 216, 896, 1048, 2188, 2212, 2324, 2440, 2509
AsuHPI GGTGA 2 cut(s) 2207, 2501
AvaII GGWCC 6 cut(s) 896, 1048, 2188, 2212, 2440, 2509
BaeGI GKGCMC 2 cut(s) 382, 417
BaeI ACNNNNGTAYC 2 cut(s) 922, 955
BamHI GGATCC 1 cut(s) 1187
BanI GGYRCC 2 cut(s) 1847, 2656
BanII GRGCYC 2 cut(s) 321, 1179
BauI CACGAG 1 cut(s) 1965
BbsI GAAGAC 1 cut(s) 1393
Bbv12I GWGCWC 3 cut(s) 321, 417, 1179
BbvCI CCTCAGC 1 cut(s) 1521
BbvI GCAGC 9 cut(s) 592, 614, 1082, 1296, 2001, 2012, 2207, 2462, 2671
BccI CCATC 9 cut(s) 212, 730, 1112, 1204, 1285, 1724, 2033, 2239, 2480
BceAI ACGGC 1 cut(s) 360
BciT130I CCWGG 2 cut(s) 894, 2193
BciVI GTATCC 1 cut(s) 670
BclI TGATCA 1 cut(s) 1921
BcoDI GTCTC 5 cut(s) 19, 68, 489, 1342, 2574
BfaI CTAG 5 cut(s) 186, 1322, 1698, 2310, 2513
BfmI CTRYAG 4 cut(s) 600, 1310, 1410, 2433
BfoI RGCGCY 1 cut(s) 641
BfrI CTTAAG 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 742
BfuI GTATCC 1 cut(s) 670
BglI GCCNNNNNGGC 1 cut(s) 347
BglII AGATCT 1 cut(s) 2022
Bme1390I CCNGG 2 cut(s) 894, 2193
Bme18I GGWCC 6 cut(s) 896, 1048, 2188, 2212, 2440, 2509
BmgBI CACGTC 1 cut(s) 1352
BmgT120I GGNCC 9 cut(s) 189, 216, 896, 1048, 2188, 2212, 2324, 2440, 2509
BmiI GGNNCC 8 cut(s) 191, 218, 1050, 1189, 1849, 2190, 2213, 2658
BmrFI CCNGG 2 cut(s) 894, 2193
BmrI ACTGGG 1 cut(s) 969
BmsI GCATC 8 cut(s) 328, 580, 1322, 1484, 1704, 1876, 2258, 2586
BmuI ACTGGG 1 cut(s) 969
BpiI GAAGAC 1 cut(s) 1393
BpmI CTGGAG 4 cut(s) 933, 1220, 1739, 2103
Bpu10I CCTNAGC 3 cut(s) 52, 531, 1521
BpuEI CTTGAG 2 cut(s) 1163, 2395
BsaBI GATNNNNATC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 4 cut(s) 516, 934, 2191, 2327
BsaWI WCCGGW 2 cut(s) 59, 2165
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 955, 985
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 286, 328, 914
Bse1I ACTGG 7 cut(s) 410, 916, 964, 1159, 1237, 2019, 2190
Bse3DI GCAATG 3 cut(s) 441, 657, 1896
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 2 cut(s) 894, 2193
BseDI CCNNGG 4 cut(s) 516, 934, 2191, 2327
BseGI GGATG 6 cut(s) 484, 835, 1144, 1818, 2249, 2491
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 4 cut(s) 46, 286, 328, 914
BseMI GCAATG 3 cut(s) 441, 657, 1896
BseNI ACTGG 7 cut(s) 410, 916, 964, 1159, 1237, 2019, 2190
BseRI GAGGAG 3 cut(s) 166, 230, 930
BseSI GKGCMC 2 cut(s) 382, 417
BseX3I CGGCCG 1 cut(s) 373
BseXI GCAGC 9 cut(s) 592, 614, 1082, 1296, 2001, 2012, 2207, 2462, 2671
BseYI CCCAGC 1 cut(s) 192
BsgI GTGCAG 4 cut(s) 87, 646, 2008, 2206
Bsh1236I CGCG 2 cut(s) 222, 396
Bsh1285I CGRYCG 2 cut(s) 376, 2166
BshNI GGYRCC 2 cut(s) 1847, 2656
BsiEI CGRYCG 2 cut(s) 376, 2166
BsiHKAI GWGCWC 3 cut(s) 321, 417, 1179
BsiSI CCGG 3 cut(s) 60, 386, 2166
BslFI GGGAC 3 cut(s) 442, 1034, 2174
BslI CCNNNNNNNGG 4 cut(s) 46, 286, 328, 914
BsmAI GTCTC 5 cut(s) 19, 68, 489, 1342, 2574
BsmBI CGTCTC 1 cut(s) 1342
BsmFI GGGAC 3 cut(s) 442, 1034, 2174
BsmI GAATGC 3 cut(s) 423, 1164, 1316
Bso31I GGTCTC 1 cut(s) 68
Bsp1286I GDGCHC 4 cut(s) 321, 382, 417, 1179
Bsp143I GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
Bsp68I TCGCGA 1 cut(s) 396
BspFNI CGCG 2 cut(s) 222, 396
BspLI GGNNCC 8 cut(s) 191, 218, 1050, 1189, 1849, 2190, 2213, 2658
BspMAI CTGCAG 3 cut(s) 604, 1314, 2437
BspMI ACCTGC 1 cut(s) 742
BspPI GGATC 4 cut(s) 543, 590, 1182, 1195
BspT107I GGYRCC 2 cut(s) 1847, 2656
BspTI CTTAAG 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 68
BsrBI CCGCTC 2 cut(s) 109, 201
BsrDI GCAATG 3 cut(s) 441, 657, 1896
BsrI ACTGG 7 cut(s) 410, 916, 964, 1159, 1237, 2019, 2190
BssECI CCNNGG 4 cut(s) 516, 934, 2191, 2327
BssMI GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
BssSI CACGAG 1 cut(s) 1965
BssT1I CCWWGG 2 cut(s) 516, 934
Bst2BI CACGAG 1 cut(s) 1965
Bst2UI CCWGG 2 cut(s) 894, 2193
Bst4CI ACNGT 7 cut(s) 401, 682, 1277, 1954, 2110, 2533, 2568
Bst6I CTCTTC 3 cut(s) 79, 1488, 2395
BstAFI CTTAAG 1 cut(s) 404
BstAPI GCANNNNNTGC 1 cut(s) 877
BstC8I GCNNGC 4 cut(s) 247, 739, 1022, 1314
BstDSI CCRYGG 1 cut(s) 2327
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 6 cut(s) 484, 835, 1144, 1818, 2249, 2491
BstFNI CGCG 2 cut(s) 222, 396
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 1 cut(s) 640
BstKTI GATC 7 cut(s) 551, 598, 1190, 1924, 2025, 2089, 2608
BstMAI GTCTC 5 cut(s) 19, 68, 489, 1342, 2574
BstMBI GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
BstMCI CGRYCG 2 cut(s) 376, 2166
BstMWI GCNNNNNNNGC 8 cut(s) 129, 135, 338, 347, 599, 877, 1714, 1816
BstNI CCWGG 2 cut(s) 894, 2193
BstNSI RCATGY 1 cut(s) 892
BstSCI CCNGG 2 cut(s) 892, 2191
BstSFI CTRYAG 4 cut(s) 600, 1310, 1410, 2433
BstSLI GKGCMC 2 cut(s) 382, 417
BstUI CGCG 2 cut(s) 222, 396
BstV1I GCAGC 9 cut(s) 592, 614, 1082, 1296, 2001, 2012, 2207, 2462, 2671
BstV2I GAAGAC 1 cut(s) 1393
BstX2I RGATCY 2 cut(s) 1187, 2022
BstYI RGATCY 2 cut(s) 1187, 2022
BstZI CGGCCG 1 cut(s) 373
BsuI GTATCC 1 cut(s) 670
BtgI CCRYGG 1 cut(s) 2327
BtgZI GCGATG 1 cut(s) 2258
BtrI CACGTC 1 cut(s) 1352
BtsCI GGATG 6 cut(s) 484, 835, 1144, 1818, 2249, 2491
BtsI GCAGTG 2 cut(s) 498, 866
BtuMI TCGCGA 1 cut(s) 396
BveI ACCTGC 1 cut(s) 742
Cac8I GCNNGC 4 cut(s) 247, 739, 1022, 1314
CaiI CAGNNNCTG 2 cut(s) 899, 2003
CfoI GCGC 1 cut(s) 640
Cfr13I GGNCC 9 cut(s) 189, 216, 896, 1048, 2188, 2212, 2324, 2440, 2509
CseI GACGC 1 cut(s) 265
Csp6I GTAC 6 cut(s) 183, 365, 1848, 1933, 2007, 2529
CviQI GTAC 6 cut(s) 183, 365, 1848, 1933, 2007, 2529
DpnI GATC 7 cut(s) 550, 597, 1189, 1923, 2024, 2088, 2607
DpnII GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
DraIII CACNNNGTG 1 cut(s) 624
EaeI YGGCCR 2 cut(s) 18, 373
EagI CGGCCG 1 cut(s) 373
Eam1104I CTCTTC 3 cut(s) 79, 1488, 2395
EarI CTCTTC 3 cut(s) 79, 1488, 2395
EciI GGCGGA 2 cut(s) 56, 125
Ecl136II GAGCTC 2 cut(s) 319, 1177
EclXI CGGCCG 1 cut(s) 373
Eco130I CCWWGG 2 cut(s) 516, 934
Eco147I AGGCCT 1 cut(s) 1416
Eco24I GRGCYC 2 cut(s) 321, 1179
Eco31I GGTCTC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 2557
Eco47I GGWCC 6 cut(s) 896, 1048, 2188, 2212, 2440, 2509
Eco52I CGGCCG 1 cut(s) 373
Eco53kI GAGCTC 2 cut(s) 319, 1177
Eco57I CTGAAG 6 cut(s) 753, 1231, 1312, 1528, 1771, 1921
EcoICRI GAGCTC 2 cut(s) 319, 1177
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 4 cut(s) 189, 216, 896, 1048
EcoRII CCWGG 2 cut(s) 892, 2191
EcoRV GATATC 1 cut(s) 2557
EcoT14I CCWWGG 2 cut(s) 516, 934
EcoT22I ATGCAT 1 cut(s) 441
EcoT38I GRGCYC 2 cut(s) 321, 1179
ErhI CCWWGG 2 cut(s) 516, 934
Esp3I CGTCTC 1 cut(s) 1342
FaqI GGGAC 3 cut(s) 442, 1034, 2174
FauI CCCGC 3 cut(s) 128, 227, 2544
FbaI TGATCA 1 cut(s) 1921
FblI GTMKAC 2 cut(s) 1956, 2162
FokI GGATG 6 cut(s) 471, 842, 1151, 1825, 2236, 2498
FriOI GRGCYC 2 cut(s) 321, 1179
FspBI CTAG 5 cut(s) 186, 1322, 1698, 2310, 2513
GlaI GCGC 1 cut(s) 639
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 4 cut(s) 933, 1220, 1739, 2103
HaeII RGCGCY 1 cut(s) 641
HapII CCGG 3 cut(s) 60, 386, 2166
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 640
Hin6I GCGC 1 cut(s) 638
HinP1I GCGC 1 cut(s) 638
HincII GTYRAC 3 cut(s) 1280, 1739, 2163
HindII GTYRAC 3 cut(s) 1280, 1739, 2163
HindIII AAGCTT 1 cut(s) 1455
HpaII CCGG 3 cut(s) 60, 386, 2166
HphI GGTGA 2 cut(s) 2207, 2501
Hpy166II GTNNAC 6 cut(s) 415, 1280, 1739, 1882, 1957, 2163
Hpy188III TCNNGA 7 cut(s) 395, 459, 472, 533, 857, 1007, 1982
Hpy8I GTNNAC 6 cut(s) 415, 1280, 1739, 1882, 1957, 2163
Hpy99I CGWCG 5 cut(s) 227, 230, 233, 236, 401
HpyAV CCTTC 4 cut(s) 752, 1542, 1795, 2122
HpyCH4III ACNGT 7 cut(s) 401, 682, 1277, 1954, 2110, 2533, 2568
HpyCH4IV ACGT 4 cut(s) 390, 970, 1351, 1826
HpyF10VI GCNNNNNNNGC 8 cut(s) 129, 135, 338, 347, 599, 877, 1714, 1816
HpySE526I ACGT 4 cut(s) 390, 970, 1351, 1826
HspAI GCGC 1 cut(s) 638
KpnI GGTACC 1 cut(s) 1851
Ksp22I TGATCA 1 cut(s) 1921
Kzo9I GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
LmnI GCTCC 4 cut(s) 201, 206, 273, 324
Lsp1109I GCAGC 9 cut(s) 592, 614, 1082, 1296, 2001, 2012, 2207, 2462, 2671
LweI GCATC 8 cut(s) 328, 580, 1322, 1484, 1704, 1876, 2258, 2586
MaeI CTAG 5 cut(s) 186, 1322, 1698, 2310, 2513
MaeII ACGT 4 cut(s) 390, 970, 1351, 1826
MaeIII GTNAC 7 cut(s) 89, 614, 1145, 1253, 1700, 1910, 2420
MalI GATC 7 cut(s) 550, 597, 1189, 1923, 2024, 2088, 2607
MbiI CCGCTC 2 cut(s) 109, 201
MboI GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
MboII GAAGA 7 cut(s) 66, 759, 1096, 1398, 1475, 1634, 2382
MfeI CAATTG 3 cut(s) 585, 1470, 1674
MflI RGATCY 2 cut(s) 1187, 2022
MhlI GDGCHC 4 cut(s) 321, 382, 417, 1179
MlyI GAGTC 2 cut(s) 284, 2595
MmeI TCCRAC 5 cut(s) 174, 932, 1215, 1642, 2446
Mph1103I ATGCAT 1 cut(s) 441
MseI TTAA 6 cut(s) 405, 1554, 1806, 1830, 2544, 2636
MslI CAYNNNNRTG 5 cut(s) 489, 503, 1157, 1917, 1923
MspA1I CMGCKG 3 cut(s) 605, 1251, 2003
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 3 cut(s) 60, 386, 2166
MspR9I CCNGG 2 cut(s) 894, 2193
MunI CAATTG 3 cut(s) 585, 1470, 1674
Mva1269I GAATGC 3 cut(s) 423, 1164, 1316
MvaI CCWGG 2 cut(s) 894, 2193
MvnI CGCG 2 cut(s) 222, 396
MwoI GCNNNNNNNGC 8 cut(s) 129, 135, 338, 347, 599, 877, 1714, 1816
NdeII GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
NlaIV GGNNCC 8 cut(s) 191, 218, 1050, 1189, 1849, 2190, 2213, 2658
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 3 cut(s) 1145, 1700, 1910
NruI TCGCGA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 441
NspI RCATGY 1 cut(s) 892
OliI CACNNNNGTG 1 cut(s) 503
PceI AGGCCT 1 cut(s) 1416
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 249, 303
PctI GAATGC 3 cut(s) 423, 1164, 1316
PleI GAGTC 2 cut(s) 284, 2595
PpsI GAGTC 2 cut(s) 284, 2595
PpuMI RGGWCCY 2 cut(s) 896, 1048
PshBI ATTAAT 1 cut(s) 2636
Psp124BI GAGCTC 2 cut(s) 321, 1179
Psp5II RGGWCCY 2 cut(s) 896, 1048
Psp6I CCWGG 2 cut(s) 892, 2191
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 2 cut(s) 892, 2191
PspN4I GGNNCC 8 cut(s) 191, 218, 1050, 1189, 1849, 2190, 2213, 2658
PspPI GGNCC 9 cut(s) 189, 216, 896, 1048, 2188, 2212, 2324, 2440, 2509
PspPPI RGGWCCY 2 cut(s) 896, 1048
PstI CTGCAG 3 cut(s) 604, 1314, 2437
PstNI CAGNNNCTG 2 cut(s) 899, 2003
PsuI RGATCY 2 cut(s) 1187, 2022
PvuII CAGCTG 3 cut(s) 605, 1251, 2003
RruI TCGCGA 1 cut(s) 396
RsaI GTAC 6 cut(s) 184, 366, 1849, 1934, 2008, 2530
RsaNI GTAC 6 cut(s) 183, 365, 1848, 1933, 2007, 2529
RseI CAYNNNNRTG 5 cut(s) 489, 503, 1157, 1917, 1923
SacI GAGCTC 2 cut(s) 321, 1179
SalI GTCGAC 1 cut(s) 2161
SaqAI TTAA 6 cut(s) 405, 1554, 1806, 1830, 2544, 2636
Sau3AI GATC 7 cut(s) 548, 595, 1187, 1921, 2022, 2086, 2605
Sau96I GGNCC 9 cut(s) 189, 216, 896, 1048, 2188, 2212, 2324, 2440, 2509
SchI GAGTC 2 cut(s) 284, 2595
ScrFI CCNGG 2 cut(s) 894, 2193
SduI GDGCHC 4 cut(s) 321, 382, 417, 1179
SfaNI GCATC 8 cut(s) 328, 580, 1322, 1484, 1704, 1876, 2258, 2586
SfcI CTRYAG 4 cut(s) 600, 1310, 1410, 2433
SinI GGWCC 6 cut(s) 896, 1048, 2188, 2212, 2440, 2509
SmiMI CAYNNNNRTG 5 cut(s) 489, 503, 1157, 1917, 1923
SmlI CTYRAG 3 cut(s) 404, 1178, 2374
SmoI CTYRAG 3 cut(s) 404, 1178, 2374
SseBI AGGCCT 1 cut(s) 1416
SspI AATATT 1 cut(s) 1530
SspMI CTAG 5 cut(s) 186, 1322, 1698, 2310, 2513
SstI GAGCTC 2 cut(s) 321, 1179
StuI AGGCCT 1 cut(s) 1416
StyD4I CCNGG 2 cut(s) 892, 2191
StyI CCWWGG 2 cut(s) 516, 934
TaaI ACNGT 7 cut(s) 401, 682, 1277, 1954, 2110, 2533, 2568
TaiI ACGT 4 cut(s) 393, 973, 1354, 1829
TaqI TCGA 5 cut(s) 955, 1712, 1843, 2162, 2604
TaqII GACCGA 2 cut(s) 659, 2457
TatI WGTACW 2 cut(s) 364, 1932
TauI GCSGC 4 cut(s) 132, 252, 423, 1995
Tru1I TTAA 6 cut(s) 405, 1554, 1806, 1830, 2544, 2636
Tru9I TTAA 6 cut(s) 405, 1554, 1806, 1830, 2544, 2636
TseFI GTSAC 3 cut(s) 1145, 1700, 1910
TseI GCWGC 9 cut(s) 602, 605, 1070, 1309, 1989, 2000, 2220, 2450, 2684
Tsp45I GTSAC 3 cut(s) 1145, 1700, 1910
TspGWI ACGGA 3 cut(s) 77, 2204, 2274
Vha464I CTTAAG 1 cut(s) 404
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 6 cut(s) 896, 1048, 2188, 2212, 2440, 2509
VspI ATTAAT 1 cut(s) 2636
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 5 cut(s) 574, 748, 1358, 1608, 1977
XceI RCATGY 1 cut(s) 892
XmiI GTMKAC 2 cut(s) 1956, 2162
XspI CTAG 5 cut(s) 186, 1322, 1698, 2310, 2513
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.