Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0010284

Physical Location & Seq

Genomic Coordinates Reverse (-)

48241110 .. 48254980

Gene Structure 13871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000029745

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2844 bp

ATGAAATCCTCAGTCTCGGCCATAACCCCTACCCCCAACATGGCCTTAGCCTCCGGTCTCCGCTTCTCTTCCTCTATCCTCCGTTACCGCCTCCCTGCACCGCTCAACTCCACCCGCTTCTGCGGCCTCCGCCACAGCCTCTCCGACCTCCGTTTCCTCTCAGCTTCCTCCTCTCGTACTAGGCCCCAGCTCCGCTCCATCAAAGCGAGGCGCCGCGACGACGACGACAACCTCCTCACAGGCGGCGAGGGGCAGAATGGGAATGGGAGCGTCCTTGTCAAGGACTCAGACGAGAGGATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCTGTACTGCTCGGCCGTGCCCTGCCGGACGTTCGCGACGGTCTTAAGCCCGTGCACCGCCGCATTCTGTATGCAATGCATGAATTGAGGCTTTCGTCCCGAAAACCATTCGTGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAGTTTCATCCTCATGGAGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCTTTACGATCCCCACTGATTCAAGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCGCTGAGCGAAGCAATGTTGCTGTCAGATTTAGAACAGGATACGGTTGATTTTGTTCCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGCCTTCCAAATTTATTATTGAATGGTTCTTCAGGGATTGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAATTGGTGGATGTTCTTAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTGGAATACATGCCAGGACCTGATTTTCCAACTGGAGGAGTGATTATGGGCAACCTTGGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTATTGTGGTCAGAGGAAAAACTGATATAGAATCTCTTGATTCTAAAGGCAAGCGAAGTGCAATTATCATAAAGGAGGTCCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATTGCACAACTTGTAGAGGATAAGATTTTAGATGGCATTAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTGATTGAGCTTAAGCGAGGATCCGACCCATCTATTGTTCTGAATAATCTATATCGCCTCACATCTCTCCAGTCCAGCTTCAGCTGTAACATGGTTGGTATTCTCAACGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCATTCCTAGACTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATTGCTGTAGGCCTTGATAATCTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCATCTCTTCTAAGAAATGAGTTCAACCTTTCTGAGAAACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTATGGAGAGGAAAAAGTTTGATAATGAAACTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAAAAACACATATTGCAGGTGGTTGAACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAAGGGTATGTTAAGCGGATGAAGCCTAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTTTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGTAGCCGCACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACATCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAATGGCTACATAAAGAAGGTGTCTTTGAGTTCTTTTTCATCAATCAGGTCAACCGGAATCATAGCAATTCAATTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTATTCGAGCCCTAGGCAGGAACACTAGAGGTTCTGTAGCCATGCGACTTAAAGAAGGCGATAAGATGGCAAGCGTAGACATTATACCAGCAGCTTTGCGGAAAGACTTGGAGAGGGTGTCAGAAGCACCAGAAAGCACGGCTAGAAGTCTGAAAGGCCCGTGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCATTCTTCCCGTCTGAACAGAGTGGGTCTAATAGGTTACAAGTTTTCTGCGGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTAGTAAGCCAAAGTGGTACTGTGAATAGAATTAAGGTGCGGGATATCTCAATACAGTCCCGCTATGCAAGGGGTGTTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAAGCTGTAATTCATGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0009295 GO:0009507 GO:0009536 GO:0016592 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseA_C DNA_topoisoIV

Protein Analysis

948

Amino Acids

104.61

Weight (kDa)

8.16

Isoelectric Point (pI)

43.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_topoisoIV	PF00521	124 - 565	5.2e-156	DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C	PF03989	599 - 645	2.5e-08	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	649 - 699	3.5e-07	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	706 - 749	3.9e-08	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	753 - 799	4e-07	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	825 - 870	9.2e-06	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	883 - 928	2.7e-06	DNA gyrase C-terminal domain, beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10690
fragaria_vesca	FvH4_7g05000 FvH4_7g05000
malus_domestica	MD02G1265100.v1.1 MD07G1054300.v1.1
prunus_persica	Prupe.2G060900_v2.0.a1 Prupe.2G060900_v2.0.a1
pyrus_communis	pycom02g22650 pycom07g04200
rosa_chinensis	RchiOBHm_Chr1g0331271
rosa_laevigata	RLG00000029745
rosa_multiflora	Rmu_co8191516.1_g000001 Rmu_sc0000597.1_g000001
rosa_roxburghii	Rroxscaffold_4G00319250
rosa_rugosa	Rorug01G0092400 Rorug01G0092500
rosa_samantha	Rh1AG117100 Rh1BG087900 Rh1CG112100 Rh1DG120900
rosa_wichuraiana	Rw1G009370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1669
Acc36I	ACCTGC	2 cut(s)	766, 1669
Acc65I	GGTACC	1 cut(s)	1871
AccB1I	GGYRCC	3 cut(s)	210, 1871, 2800
AccBSI	CCGCTC	2 cut(s)	103, 195
AccI	GTMKAC	2 cut(s)	1980, 2388
AccII	CGCG	2 cut(s)	216, 390
AclWI	GGATC	5 cut(s)	305, 567, 614, 1206, 1219
AcoI	YGGCCR	2 cut(s)	18, 367
AcsI	RAATTY	5 cut(s)	598, 772, 1382, 1632, 2001
AcuI	CTGAAG	6 cut(s)	777, 1255, 1336, 1552, 1795, 1945
AcyI	GRCGYC	1 cut(s)	211
AdeI	CACNNNGTG	1 cut(s)	648
AfaI	GTAC	6 cut(s)	178, 360, 1873, 1958, 2032, 2674
AfiI	CCNNNNNNNGG	5 cut(s)	40, 280, 322, 938, 2328
AflII	CTTAAG	2 cut(s)	398, 1202
AflIII	ACRYGT	1 cut(s)	1848
AjiI	CACGTC	1 cut(s)	1376
AjnI	CCWGG	2 cut(s)	916, 2215
AleI	CACNNNNGTG	1 cut(s)	527
AloI	GAACNNNNNNTCC	6 cut(s)	589, 621, 2496, 2528, 2637, 2669
Alw21I	GWGCWC	2 cut(s)	315, 411
Alw26I	GTCTC	4 cut(s)	19, 62, 513, 1366
Alw44I	GTGCAC	1 cut(s)	407
AlwI	GGATC	5 cut(s)	305, 567, 614, 1206, 1219
AlwNI	CAGNNNCTG	2 cut(s)	923, 2027
ApaLI	GTGCAC	1 cut(s)	407
ApeKI	GCWGC	8 cut(s)	626, 629, 1094, 1333, 2024, 2244, 2402, 2594
ApoI	RAATTY	5 cut(s)	598, 772, 1382, 1632, 2001
AseI	ATTAAT	1 cut(s)	2780
Asp718I	GGTACC	1 cut(s)	1871
AspA2I	CCTAGG	1 cut(s)	2323
AspLEI	GCGC	2 cut(s)	213, 664
AspS9I	GGNCC	8 cut(s)	183, 920, 1072, 2212, 2236, 2468, 2584, 2653
AsuHPI	GGTGA	2 cut(s)	2231, 2645
AvaII	GGWCC	6 cut(s)	920, 1072, 2212, 2236, 2584, 2653
AvrII	CCTAGG	1 cut(s)	2323
BaeGI	GKGCMC	2 cut(s)	376, 411
BaeI	ACNNNNGTAYC	2 cut(s)	946, 979
BamHI	GGATCC	1 cut(s)	1211
BanI	GGYRCC	3 cut(s)	210, 1871, 2800
BanII	GRGCYC	2 cut(s)	315, 2323
BauI	CACGAG	1 cut(s)	1989
BbsI	GAAGAC	1 cut(s)	1417
Bbv12I	GWGCWC	2 cut(s)	315, 411
BbvCI	CCTCAGC	1 cut(s)	1545
BbvI	GCAGC	8 cut(s)	616, 638, 1106, 1320, 2036, 2231, 2414, 2606
BceAI	ACGGC	2 cut(s)	354, 2466
BciT130I	CCWGG	2 cut(s)	918, 2217
BciVI	GTATCC	1 cut(s)	694
BclI	TGATCA	1 cut(s)	1945
BcoDI	GTCTC	4 cut(s)	19, 62, 513, 1366
BfaI	CTAG	9 cut(s)	180, 477, 497, 1346, 1722, 2324, 2337, 2454, 2657
BfmI	CTRYAG	4 cut(s)	624, 1334, 1434, 2346
BfoI	RGCGCY	2 cut(s)	214, 665
BfrI	CTTAAG	2 cut(s)	398, 1202
BfuAI	ACCTGC	2 cut(s)	766, 1669
BfuI	GTATCC	1 cut(s)	694
BglI	GCCNNNNNGGC	1 cut(s)	341
BglII	AGATCT	1 cut(s)	2046
BlnI	CCTAGG	1 cut(s)	2323
BlpI	GCTNAGC	1 cut(s)	665
Bme1390I	CCNGG	2 cut(s)	918, 2217
Bme18I	GGWCC	6 cut(s)	920, 1072, 2212, 2236, 2584, 2653
BmgBI	CACGTC	1 cut(s)	1376
BmgT120I	GGNCC	8 cut(s)	183, 920, 1072, 2212, 2236, 2468, 2584, 2653
BmiI	GGNNCC	8 cut(s)	185, 212, 1074, 1213, 1873, 2214, 2237, 2802
BmrFI	CCNGG	2 cut(s)	918, 2217
BmrI	ACTGGG	1 cut(s)	993
BmsI	GCATC	8 cut(s)	322, 604, 1346, 1508, 1728, 1900, 2282, 2730
BmuI	ACTGGG	1 cut(s)	993
BpiI	GAAGAC	1 cut(s)	1417
BpmI	CTGGAG	4 cut(s)	957, 1244, 1763, 2127
Bpu10I	CCTNAGC	3 cut(s)	46, 555, 1545
Bpu1102I	GCTNAGC	1 cut(s)	665
BpuEI	CTTGAG	1 cut(s)	2539
BsaBI	GATNNNNATC	1 cut(s)	618
BsaHI	GRCGYC	1 cut(s)	211
BsaI	GGTCTC	1 cut(s)	62
BsaJI	CCNNGG	5 cut(s)	540, 958, 2215, 2323, 2471
BsaWI	WCCGGW	2 cut(s)	53, 2189
BsaXI	ACNNNNNCTCC	6 cut(s)	125, 155, 979, 1009, 2414, 2444
Bsc4I	CCNNNNNNNGG	5 cut(s)	40, 280, 322, 938, 2328
Bse1I	ACTGG	5 cut(s)	940, 988, 1183, 1261, 2043
Bse3DI	GCAATG	3 cut(s)	435, 681, 1920
Bse8I	GATNNNNATC	1 cut(s)	618
BseBI	CCWGG	2 cut(s)	918, 2217
BseDI	CCNNGG	5 cut(s)	540, 958, 2215, 2323, 2471
BseGI	GGATG	6 cut(s)	508, 859, 1168, 1842, 2273, 2635
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	5 cut(s)	40, 280, 322, 938, 2328
BseMI	GCAATG	3 cut(s)	435, 681, 1920
BseNI	ACTGG	5 cut(s)	940, 988, 1183, 1261, 2043
BseRI	GAGGAG	3 cut(s)	160, 224, 954
BseSI	GKGCMC	2 cut(s)	376, 411
BseX3I	CGGCCG	1 cut(s)	367
BseXI	GCAGC	8 cut(s)	616, 638, 1106, 1320, 2036, 2231, 2414, 2606
BseYI	CCCAGC	1 cut(s)	186
BsgI	GTGCAG	3 cut(s)	81, 670, 2230
Bsh1236I	CGCG	2 cut(s)	216, 390
Bsh1285I	CGRYCG	1 cut(s)	370
BshNI	GGYRCC	3 cut(s)	210, 1871, 2800
BsiEI	CGRYCG	1 cut(s)	370
BsiHKAI	GWGCWC	2 cut(s)	315, 411
BsiSI	CCGG	3 cut(s)	54, 380, 2190
BslFI	GGGAC	4 cut(s)	436, 1058, 2198, 2698
BslI	CCNNNNNNNGG	5 cut(s)	40, 280, 322, 938, 2328
BsmAI	GTCTC	4 cut(s)	19, 62, 513, 1366
BsmBI	CGTCTC	1 cut(s)	1366
BsmFI	GGGAC	4 cut(s)	436, 1058, 2198, 2698
BsmI	GAATGC	3 cut(s)	417, 1188, 1340
Bso31I	GGTCTC	1 cut(s)	62
Bsp1286I	GDGCHC	4 cut(s)	315, 376, 411, 2323
Bsp143I	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
Bsp1720I	GCTNAGC	1 cut(s)	665
Bsp68I	TCGCGA	1 cut(s)	390
BspFNI	CGCG	2 cut(s)	216, 390
BspLI	GGNNCC	8 cut(s)	185, 212, 1074, 1213, 1873, 2214, 2237, 2802
BspMAI	CTGCAG	2 cut(s)	628, 1338
BspMI	ACCTGC	2 cut(s)	766, 1669
BspPI	GGATC	5 cut(s)	305, 567, 614, 1206, 1219
BspT107I	GGYRCC	3 cut(s)	210, 1871, 2800
BspTI	CTTAAG	2 cut(s)	398, 1202
BspTNI	GGTCTC	1 cut(s)	62
BsrBI	CCGCTC	2 cut(s)	103, 195
BsrDI	GCAATG	3 cut(s)	435, 681, 1920
BsrI	ACTGG	5 cut(s)	940, 988, 1183, 1261, 2043
BssECI	CCNNGG	5 cut(s)	540, 958, 2215, 2323, 2471
BssMI	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
BssNI	GRCGYC	1 cut(s)	211
BssSI	CACGAG	1 cut(s)	1989
BssT1I	CCWWGG	3 cut(s)	540, 958, 2323
Bst2BI	CACGAG	1 cut(s)	1989
Bst2UI	CCWGG	2 cut(s)	918, 2217
Bst4CI	ACNGT	7 cut(s)	395, 706, 1301, 1978, 2134, 2677, 2712
Bst6I	CTCTTC	2 cut(s)	73, 1512
BstACI	GRCGYC	1 cut(s)	211
BstAFI	CTTAAG	2 cut(s)	398, 1202
BstAPI	GCANNNNNTGC	1 cut(s)	901
BstC8I	GCNNGC	4 cut(s)	763, 1046, 1338, 2383
BstDSI	CCRYGG	1 cut(s)	2471
BstENI	CCTNNNNNAGG	1 cut(s)	278
BstF5I	GGATG	6 cut(s)	508, 859, 1168, 1842, 2273, 2635
BstFNI	CGCG	2 cut(s)	216, 390
BstH2I	RGCGCY	2 cut(s)	214, 665
BstHHI	GCGC	2 cut(s)	213, 664
BstKTI	GATC	8 cut(s)	300, 575, 622, 1214, 1948, 2049, 2113, 2752
BstMAI	GTCTC	4 cut(s)	19, 62, 513, 1366
BstMBI	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
BstMCI	CGRYCG	1 cut(s)	370
BstMWI	GCNNNNNNNGC	8 cut(s)	123, 129, 332, 341, 623, 901, 1738, 1840
BstNI	CCWGG	2 cut(s)	918, 2217
BstNSI	RCATGY	1 cut(s)	916
BstSCI	CCNGG	2 cut(s)	916, 2215
BstSFI	CTRYAG	4 cut(s)	624, 1334, 1434, 2346
BstSLI	GKGCMC	2 cut(s)	376, 411
BstUI	CGCG	2 cut(s)	216, 390
BstV1I	GCAGC	8 cut(s)	616, 638, 1106, 1320, 2036, 2231, 2414, 2606
BstV2I	GAAGAC	1 cut(s)	1417
BstX2I	RGATCY	2 cut(s)	1211, 2046
BstYI	RGATCY	2 cut(s)	1211, 2046
BstZI	CGGCCG	1 cut(s)	367
BsuI	GTATCC	1 cut(s)	694
BtgI	CCRYGG	1 cut(s)	2471
BtgZI	GCGATG	1 cut(s)	2282
BtrI	CACGTC	1 cut(s)	1376
BtsCI	GGATG	6 cut(s)	508, 859, 1168, 1842, 2273, 2635
BtsI	GCAGTG	2 cut(s)	522, 890
BtsIMutI	CAGTG	8 cut(s)	522, 578, 890, 1176, 1819, 1969, 2036, 2139
BtuMI	TCGCGA	1 cut(s)	390
BveI	ACCTGC	2 cut(s)	766, 1669
Cac8I	GCNNGC	4 cut(s)	763, 1046, 1338, 2383
CaiI	CAGNNNCTG	2 cut(s)	923, 2027
CfoI	GCGC	2 cut(s)	213, 664
Cfr13I	GGNCC	8 cut(s)	183, 920, 1072, 2212, 2236, 2468, 2584, 2653
CseI	GACGC	1 cut(s)	259
Csp6I	GTAC	6 cut(s)	177, 359, 1872, 1957, 2031, 2673
CviQI	GTAC	6 cut(s)	177, 359, 1872, 1957, 2031, 2673
DinI	GGCGCC	1 cut(s)	212
DpnI	GATC	8 cut(s)	299, 574, 621, 1213, 1947, 2048, 2112, 2751
DpnII	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
DraIII	CACNNNGTG	1 cut(s)	648
EaeI	YGGCCR	2 cut(s)	18, 367
EagI	CGGCCG	1 cut(s)	367
Eam1104I	CTCTTC	2 cut(s)	73, 1512
EarI	CTCTTC	2 cut(s)	73, 1512
EciI	GGCGGA	1 cut(s)	119
Ecl136II	GAGCTC	1 cut(s)	313
EclXI	CGGCCG	1 cut(s)	367
Eco130I	CCWWGG	3 cut(s)	540, 958, 2323
Eco147I	AGGCCT	1 cut(s)	1440
Eco24I	GRGCYC	2 cut(s)	315, 2323
Eco31I	GGTCTC	1 cut(s)	62
Eco32I	GATATC	1 cut(s)	2701
Eco47I	GGWCC	6 cut(s)	920, 1072, 2212, 2236, 2584, 2653
Eco52I	CGGCCG	1 cut(s)	367
Eco53kI	GAGCTC	1 cut(s)	313
Eco57I	CTGAAG	6 cut(s)	777, 1255, 1336, 1552, 1795, 1945
EcoICRI	GAGCTC	1 cut(s)	313
EcoNI	CCTNNNNNAGG	1 cut(s)	278
EcoO109I	RGGNCCY	3 cut(s)	183, 920, 1072
EcoRII	CCWGG	2 cut(s)	916, 2215
EcoRV	GATATC	1 cut(s)	2701
EcoT14I	CCWWGG	3 cut(s)	540, 958, 2323
EcoT22I	ATGCAT	1 cut(s)	435
EcoT38I	GRGCYC	2 cut(s)	315, 2323
EgeI	GGCGCC	1 cut(s)	212
EheI	GGCGCC	1 cut(s)	212
ErhI	CCWWGG	3 cut(s)	540, 958, 2323
Esp3I	CGTCTC	1 cut(s)	1366
FaqI	GGGAC	4 cut(s)	436, 1058, 2198, 2698
FauI	CCCGC	3 cut(s)	122, 2688, 2723
FbaI	TGATCA	1 cut(s)	1945
FblI	GTMKAC	2 cut(s)	1980, 2388
FokI	GGATG	6 cut(s)	495, 866, 1175, 1849, 2260, 2642
FriOI	GRGCYC	2 cut(s)	315, 2323
FspBI	CTAG	9 cut(s)	180, 477, 497, 1346, 1722, 2324, 2337, 2454, 2657
GlaI	GCGC	2 cut(s)	212, 663
GsaI	CCCAGC	1 cut(s)	190
GsuI	CTGGAG	4 cut(s)	957, 1244, 1763, 2127
HaeII	RGCGCY	2 cut(s)	214, 665
HapII	CCGG	3 cut(s)	54, 380, 2190
HgaI	GACGC	1 cut(s)	259
HhaI	GCGC	2 cut(s)	213, 664
Hin1I	GRCGYC	1 cut(s)	211
Hin6I	GCGC	2 cut(s)	211, 662
HinP1I	GCGC	2 cut(s)	211, 662
HincII	GTYRAC	3 cut(s)	1304, 1763, 2187
HindII	GTYRAC	3 cut(s)	1304, 1763, 2187
HindIII	AAGCTT	1 cut(s)	1479
HpaII	CCGG	3 cut(s)	54, 380, 2190
HphI	GGTGA	2 cut(s)	2231, 2645
Hpy166II	GTNNAC	7 cut(s)	409, 1304, 1763, 1906, 1981, 2187, 2389
Hpy188III	TCNNGA	7 cut(s)	389, 453, 466, 557, 881, 1031, 2006
Hpy8I	GTNNAC	7 cut(s)	409, 1304, 1763, 1906, 1981, 2187, 2389
Hpy99I	CGWCG	4 cut(s)	221, 224, 227, 395
HpyAV	CCTTC	5 cut(s)	776, 1566, 1819, 2146, 2360
HpyCH4III	ACNGT	7 cut(s)	395, 706, 1301, 1978, 2134, 2677, 2712
HpyCH4IV	ACGT	4 cut(s)	384, 994, 1375, 1850
HpyF10VI	GCNNNNNNNGC	8 cut(s)	123, 129, 332, 341, 623, 901, 1738, 1840
HpySE526I	ACGT	4 cut(s)	384, 994, 1375, 1850
Hsp92I	GRCGYC	1 cut(s)	211
HspAI	GCGC	2 cut(s)	211, 662
KasI	GGCGCC	1 cut(s)	210
KpnI	GGTACC	1 cut(s)	1875
Ksp22I	TGATCA	1 cut(s)	1945
Kzo9I	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
LmnI	GCTCC	4 cut(s)	195, 200, 267, 318
Lsp1109I	GCAGC	8 cut(s)	616, 638, 1106, 1320, 2036, 2231, 2414, 2606
LweI	GCATC	8 cut(s)	322, 604, 1346, 1508, 1728, 1900, 2282, 2730
MaeI	CTAG	9 cut(s)	180, 477, 497, 1346, 1722, 2324, 2337, 2454, 2657
MaeII	ACGT	4 cut(s)	384, 994, 1375, 1850
MaeIII	GTNAC	7 cut(s)	83, 638, 1169, 1277, 1724, 1934, 2564
MalI	GATC	8 cut(s)	299, 574, 621, 1213, 1947, 2048, 2112, 2751
MbiI	CCGCTC	2 cut(s)	103, 195
MboI	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
MboII	GAAGA	7 cut(s)	60, 783, 1120, 1422, 1499, 1658, 2526
MfeI	CAATTG	4 cut(s)	609, 1494, 1698, 2207
MflI	RGATCY	2 cut(s)	1211, 2046
MhlI	GDGCHC	4 cut(s)	315, 376, 411, 2323
Mly113I	GGCGCC	1 cut(s)	211
MlyI	GAGTC	2 cut(s)	278, 2739
MmeI	TCCRAC	5 cut(s)	168, 466, 956, 1239, 2590
Mph1103I	ATGCAT	1 cut(s)	435
MseI	TTAA	9 cut(s)	399, 1203, 1578, 1830, 1854, 2361, 2688, 2780, 2842
MslI	CAYNNNNRTG	5 cut(s)	513, 527, 1181, 1941, 1947
MspA1I	CMGCKG	3 cut(s)	629, 1275, 2027
MspCI	CTTAAG	2 cut(s)	398, 1202
MspI	CCGG	3 cut(s)	54, 380, 2190
MspR9I	CCNGG	2 cut(s)	918, 2217
MunI	CAATTG	4 cut(s)	609, 1494, 1698, 2207
Mva1269I	GAATGC	3 cut(s)	417, 1188, 1340
MvaI	CCWGG	2 cut(s)	918, 2217
MvnI	CGCG	2 cut(s)	216, 390
MwoI	GCNNNNNNNGC	8 cut(s)	123, 129, 332, 341, 623, 901, 1738, 1840
NarI	GGCGCC	1 cut(s)	211
NdeII	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
NlaIV	GGNNCC	8 cut(s)	185, 212, 1074, 1213, 1873, 2214, 2237, 2802
NmeAIII	GCCGAG	1 cut(s)	345
NmuCI	GTSAC	3 cut(s)	1169, 1724, 1934
NruI	TCGCGA	1 cut(s)	390
NsiI	ATGCAT	1 cut(s)	435
NspI	RCATGY	1 cut(s)	916
OliI	CACNNNNGTG	1 cut(s)	527
PaqCI	CACCTGC	1 cut(s)	1669
PceI	AGGCCT	1 cut(s)	1440
PcsI	WCGNNNNNNNCGW	1 cut(s)	297
PctI	GAATGC	3 cut(s)	417, 1188, 1340
PfeI	GAWTC	9 cut(s)	536, 583, 871, 1025, 1034, 1616, 2193, 2613, 2764
PleI	GAGTC	2 cut(s)	278, 2739
PluTI	GGCGCC	1 cut(s)	214
PpsI	GAGTC	2 cut(s)	278, 2739
PpuMI	RGGWCCY	2 cut(s)	920, 1072
PshBI	ATTAAT	1 cut(s)	2780
Psp124BI	GAGCTC	1 cut(s)	315
Psp5II	RGGWCCY	2 cut(s)	920, 1072
Psp6I	CCWGG	2 cut(s)	916, 2215
PspFI	CCCAGC	1 cut(s)	186
PspGI	CCWGG	2 cut(s)	916, 2215
PspN4I	GGNNCC	8 cut(s)	185, 212, 1074, 1213, 1873, 2214, 2237, 2802
PspPI	GGNCC	8 cut(s)	183, 920, 1072, 2212, 2236, 2468, 2584, 2653
PspPPI	RGGWCCY	2 cut(s)	920, 1072
PstI	CTGCAG	2 cut(s)	628, 1338
PstNI	CAGNNNCTG	2 cut(s)	923, 2027
PsuI	RGATCY	2 cut(s)	1211, 2046
PvuII	CAGCTG	3 cut(s)	629, 1275, 2027
RruI	TCGCGA	1 cut(s)	390
RsaI	GTAC	6 cut(s)	178, 360, 1873, 1958, 2032, 2674
RsaNI	GTAC	6 cut(s)	177, 359, 1872, 1957, 2031, 2673
RseI	CAYNNNNRTG	5 cut(s)	513, 527, 1181, 1941, 1947
SacI	GAGCTC	1 cut(s)	315
SaqAI	TTAA	9 cut(s)	399, 1203, 1578, 1830, 1854, 2361, 2688, 2780, 2842
Sau3AI	GATC	8 cut(s)	297, 572, 619, 1211, 1945, 2046, 2110, 2749
Sau96I	GGNCC	8 cut(s)	183, 920, 1072, 2212, 2236, 2468, 2584, 2653
SchI	GAGTC	2 cut(s)	278, 2739
ScrFI	CCNGG	2 cut(s)	918, 2217
SduI	GDGCHC	4 cut(s)	315, 376, 411, 2323
SfaNI	GCATC	8 cut(s)	322, 604, 1346, 1508, 1728, 1900, 2282, 2730
SfcI	CTRYAG	4 cut(s)	624, 1334, 1434, 2346
SfoI	GGCGCC	1 cut(s)	212
SinI	GGWCC	6 cut(s)	920, 1072, 2212, 2236, 2584, 2653
SmiMI	CAYNNNNRTG	5 cut(s)	513, 527, 1181, 1941, 1947
SmlI	CTYRAG	3 cut(s)	398, 1202, 2518
SmoI	CTYRAG	3 cut(s)	398, 1202, 2518
SseBI	AGGCCT	1 cut(s)	1440
SspDI	GGCGCC	1 cut(s)	210
SspI	AATATT	1 cut(s)	1554
SspMI	CTAG	9 cut(s)	180, 477, 497, 1346, 1722, 2324, 2337, 2454, 2657
SstI	GAGCTC	1 cut(s)	315
StuI	AGGCCT	1 cut(s)	1440
StyD4I	CCNGG	2 cut(s)	916, 2215
StyI	CCWWGG	3 cut(s)	540, 958, 2323
TaaI	ACNGT	7 cut(s)	395, 706, 1301, 1978, 2134, 2677, 2712
TaiI	ACGT	4 cut(s)	387, 997, 1378, 1853
TaqI	TCGA	5 cut(s)	979, 1736, 1867, 2317, 2748
TaqII	GACCGA	1 cut(s)	2601
TatI	WGTACW	2 cut(s)	358, 1956
TauI	GCSGC	5 cut(s)	126, 216, 246, 417, 2019
TfiI	GAWTC	9 cut(s)	536, 583, 871, 1025, 1034, 1616, 2193, 2613, 2764
Tru1I	TTAA	9 cut(s)	399, 1203, 1578, 1830, 1854, 2361, 2688, 2780, 2842
Tru9I	TTAA	9 cut(s)	399, 1203, 1578, 1830, 1854, 2361, 2688, 2780, 2842
TscAI	CASTG	8 cut(s)	529, 585, 897, 1183, 1819, 1969, 2043, 2139
TseFI	GTSAC	3 cut(s)	1169, 1724, 1934
TseI	GCWGC	8 cut(s)	626, 629, 1094, 1333, 2024, 2244, 2402, 2594
Tsp45I	GTSAC	3 cut(s)	1169, 1724, 1934
TspGWI	ACGGA	4 cut(s)	71, 140, 2228, 2298
TspRI	CASTG	8 cut(s)	529, 585, 897, 1183, 1819, 1969, 2043, 2139
Vha464I	CTTAAG	2 cut(s)	398, 1202
VneI	GTGCAC	1 cut(s)	407
VpaK11BI	GGWCC	6 cut(s)	920, 1072, 2212, 2236, 2584, 2653
VspI	ATTAAT	1 cut(s)	2780
XagI	CCTNNNNNAGG	1 cut(s)	278
XapI	RAATTY	5 cut(s)	598, 772, 1382, 1632, 2001
XceI	RCATGY	1 cut(s)	916
XmaJI	CCTAGG	1 cut(s)	2323
XmiI	GTMKAC	2 cut(s)	1980, 2388
XspI	CTAG	9 cut(s)	180, 477, 497, 1346, 1722, 2324, 2337, 2454, 2657
Zsp2I	ATGCAT	1 cut(s)	435

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.